RchiOBHm_Chr2g0085731

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016668
Physical Location & Seq
Reverse (-)
1040047 .. 1043404
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46128

Sequence Viewer

Length: 2574 bp
ATGGGTTTCAACTTCTTTGCTTCTCTACTGGTTCTTTCACTGTTGTTGAAGCCATTTGCTTGTCAACAACCCAACACAGATGGGTTTTATGTCTCTCAGTTCTTGCAAAAGATGGGTTTGTCTTCTTCTTCTTCACTTTACAACTTCTCTGCTCCTGTTTGTTCTTGGTCTGGAGTATTCTGTGATTCAGAAAATGAATATGTCATTCACTTAGTTGCTTCTGGCTTAGAACTCTCTGGTTCTATCCCTGATACCACTATTGGGAAGCTAACAAAGCTTCAATCTTTGGACCTTAGCAACAACAAAATCACTGGTTTGCCATCAGATTTGTGGAGCTTAGGCTCACTCAAGAACCTTAATCTCTCCCATAACCAGATTTCTGGGTCCTTGCCTAACAACATTGGCAACTTTGGCCTACTTGAAAGCTTTGATATTTCAAGTAACAATTTTTCTGGTGAGATTCCTGCAGATATAAGCTCACTTACCAGTCTCAGAGTTCTTAGGCTCAGTCAAAATGGGTTTGAAATGGGCATTCCTTCAGGAATTGTGGGTTGCCAATCTTTGGTTGAGATTGATCTTTCTTCGAATAGATTGAATGGGTCTCTCCCAGATAGTTTTGGTGCTGCTTTTACTAAGCTCAAAAGTTTGAATCTTGCTGGAAATGAGATTCATGGCAGGGACTCTGATTTGTCAGAGATGAAATCCATTACTAGTCTTAACATTTCTGGGAATCTGTTCAAGGGTTCAGTGATGGGGGTGTTAATGGAGCTGTTGGAGGTGATTGACCTGAGCAAGAACCAGTTTGAAGGTCACATTTCTCAGGTACAATTCAATGCTAGCTATAATTGGTCTCATTTGGTTTATTTAGACCTGTCTGAGAATCAACTTGGTGGAGAACTTTTTCATAATTTAACTGAAGCCCAAAATCTAAAGCACCTGAATCTAGCGCACAATAGATTTACTAGGCATGCATTCCCTAGTGTTGAAATGTTTCCTAGTTTGGAATATCTTAATTTGTCGAAAACTAGTCTTACTGGTCAGATTCCTGATGAACTTTCTCAGTTGAGTAGCTTGAGTACACTTGATCTTTCTGAGAACCATCTGATAGGTCAAATTCCATTCCTGAGTCTAGAGAGTCTCCAAGTCCTTGATGTTTCACTCAACAATTTGAGTGGAGAAGTCCCGGTTTCGCTGTTGGAGAAACTTCCTTGGATGAAGAGGTTCAACTTCTCTTACAATAACTTGACACTTTGTGCTTCTGAAATCTCCACCGAAACCCTCAAAAAATCTTTCTTTGGTGCTTCAAACAGCTGCCCCATTGCCGCAAACCCAGCCCTTCTTAGAAGTCAAGCAGCTAAGGATAACCACAAGGGACTAAAGCTTGCATTGGCTTTGGCCCTCTCAATGGTGTGTTTGCTTGTTGGTTTGCTATTCCTAGCAGTTGGTTGCAGAAGGAAAACCAGAATGTGGGAAGTAAAGCCAACCTCATACAAGGATGAACAAACTATATCTGGCCCCTTTTCCTTCCAGACTGACTCCACTACTTGGGTGGCAGATGTTAAGCAAGCAACATCAGTCCTTGTAGTGATTTTCGAGAAGCCATTGTTGAACTTCACATTCGCAGACCTCTTGTCAGCAACTTCAAATTTTGACCGCGGAACACTTTTGGCTGAAGGGAAGTTTGGGCCTGTTTACAGAGGATTTCTGCCAGGTGGAATTCATGTAGCAGTGAAAGTTTTAGTGCATGGTTCTACATTGACAGACCAGGAAGCTGCAAGGGAGTTTGAGTATCTTGGTCGAATCAAGCACCCCAATCTTGTTCCATTGACTGGATATTGCTTAGCTGGGGAGCAGAGAATTGCTATCTATGATTACATGGAGAATGGGAACCTTCAGAATTTGCTTCATGACTTGCCGCTTGGTGTTCAAACAACTGAGGACTGGAGCACAGATACTTGGGAAGAAGATGACAACAATGGCATTCAACATGTTGGTTCTGAAGGGTTGTTAACAAGTTGGAGGTTCCGTCACAAGATTGCTCTAGGTGCTGCCCGAGCATTGGCATTTCTACACCATGGTTGCTCACCTCCAATTATTCATAGAGATGTCAAGGCTAGTAGTGTTTATCTGGATTATAACTTGGAGCCAAGGCTATCTGATTTTGGACTGGCTAAGATTTTCGGTAGTGGTTTAGATGAAGAGATCTCTCTCGGCTCTCCTGGCTATGTGCCACCAGAGTTTACTCAGGCAGAATGTGATTCTCTAACACCAAAATCTGATGTATATTGCTTTGGAGTTGTTCTTTTTGAGCTCATTACTGGGAAAAAACCATCTGGTGATGATTATCCGGAGGAGAAAGAAGCAACTCTAGTGAGCTGGGTCAGAGGATTGGTTAGGAAGAACAAAGTGTCTAGCGCTATTGATCCGAAAATTCGTGATACAGGACGTGATGACCAAATGGAGGAGGCACTCAAGATTGGATATCTGTGCACAGCTGACCTTCCCTCAAAGCGACCAAGCATGCAACAGATAGTTGGACTTCTTAAAGATATTGAACCAGCAGGTAACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

857

Amino Acids

93.97

Weight (kDa)

5.3

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 81 - 126 7e-10 Leucine rich repeat
LRR_14 PF23598 85 - 168 6.9e-10 Leucine-rich repeat region
LRR_14 PF23598 109 - 338 6.2e-12 Leucine-rich repeat region
LRR_8 PF13855 187 - 245 2.6e-06 Leucine rich repeat
LRR_8 PF13855 309 - 368 9.2e-08 Leucine rich repeat
Pkinase PF00069 553 - 845 5.6e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 554 - 846 5.4e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016668)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24230
fragaria_vesca FvH4_1g01000
malus_domestica MD02G1008100.v1.1 MD15G1152400.v1.1
prunus_persica Prupe.7G262300_v2.0.a1
pyrus_communis pycom15g13700
rosa_chinensis RchiOBHm_Chr2g0085731
rosa_laevigata RLG00000015715
rosa_multiflora Rmu_sc0009759.1_g000005 Rmu_sc0023131.1_g000002 Rmu_sc0033740.1_g000001
rosa_rugosa Rorug01G0462800
rosa_samantha Rh2CG013300
rosa_wichuraiana Rw2G001060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2136
Acc36I ACCTGC 1 cut(s) 2552
AccB7I CCANNNNNTGG 4 cut(s) 562, 1467, 1545, 1829
AccII CGCG 1 cut(s) 1656
AccIII TCCGGA 1 cut(s) 2347
AciI CCGC 4 cut(s) 1323, 1654, 1656, 1916
AclWI GGATC 1 cut(s) 2417
AcsI RAATTY 5 cut(s) 1113, 1645, 1716, 1897, 2430
AcuI CTGAAG 5 cut(s) 522, 936, 1692, 1877, 2019
AdeI CACNNNGTG 1 cut(s) 1253
AfaI GTAC 2 cut(s) 825, 1078
AfeI AGCGCT 1 cut(s) 2416
AfiI CCNNNNNNNGG 8 cut(s) 261, 562, 1405, 1467, 1545, 1829, 2059, 2461
AflIII ACRYGT 1 cut(s) 1987
AhdI GACNNNNNGTC 1 cut(s) 1630
AhlI ACTAGT 2 cut(s) 710, 1025
AjiI CACGTC 1 cut(s) 2447
AjnI CCWGG 3 cut(s) 1708, 1764, 2218
AjuI GAANNNNNNNTTGG 2 cut(s) 1665, 1697
Alw21I GWGCWC 3 cut(s) 1949, 2313, 2492
Alw26I GTCTC 5 cut(s) 97, 494, 606, 855, 1142
Alw44I GTGCAC 1 cut(s) 2488
AlwI GGATC 1 cut(s) 2417
Ama87I CYCGRG 1 cut(s) 2052
Aor13HI TCCGGA 1 cut(s) 2347
Aor51HI AGCGCT 1 cut(s) 2416
AoxI GGCC 4 cut(s) 412, 1395, 1513, 1685
ApaLI GTGCAC 1 cut(s) 2488
ApeKI GCWGC 5 cut(s) 623, 1311, 1352, 1772, 2048
ApoI RAATTY 5 cut(s) 1113, 1645, 1716, 1897, 2430
Asp700I GAANNNNTTC 4 cut(s) 734, 900, 990, 1220
AspLEI GCGC 2 cut(s) 949, 2417
AspS9I GGNCC 5 cut(s) 289, 384, 1396, 1514, 1685
AsuC2I CCSGG 1 cut(s) 1184
AsuHPI GGTGA 4 cut(s) 467, 790, 2076, 2348
AsuII TTCGAA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 836
AvaI CYCGRG 1 cut(s) 2052
AvaII GGWCC 2 cut(s) 289, 384
BaeGI GKGCMC 1 cut(s) 2492
BanII GRGCYC 1 cut(s) 2313
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 3 cut(s) 1949, 2313, 2492
BbvI GCAGC 5 cut(s) 610, 1298, 1364, 1759, 2035
BccI CCATC 6 cut(s) 74, 106, 328, 745, 1107, 2338
BciT130I CCWGG 3 cut(s) 1710, 1766, 2220
BcnI CCSGG 1 cut(s) 1184
BcoDI GTCTC 5 cut(s) 97, 494, 606, 855, 1142
BcuI ACTAGT 2 cut(s) 710, 1025
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 2418
BfuAI ACCTGC 1 cut(s) 2552
BglII AGATCT 1 cut(s) 2202
BisI GCNGC 7 cut(s) 624, 1312, 1323, 1353, 1773, 1916, 2049
BlpI GCTNAGC 1 cut(s) 1840
BlsI GCNGC 7 cut(s) 625, 1313, 1324, 1354, 1774, 1917, 2050
Bme1390I CCNGG 4 cut(s) 1184, 1710, 1766, 2220
Bme18I GGWCC 2 cut(s) 289, 384
BmeRI GACNNNNNGTC 1 cut(s) 1630
BmeT110I CYCGRG 1 cut(s) 2052
BmgBI CACGTC 1 cut(s) 2447
BmgT120I GGNCC 5 cut(s) 289, 384, 1396, 1514, 1685
BmiI GGNNCC 5 cut(s) 385, 1516, 1889, 2024, 2145
BmrFI CCNGG 4 cut(s) 1184, 1710, 1766, 2220
BmrI ACTGGG 1 cut(s) 2328
BmtI GCTAGC 1 cut(s) 840
BmuI ACTGGG 1 cut(s) 2328
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 2 cut(s) 192, 1963
Bpu10I CCTNAGC 4 cut(s) 293, 337, 788, 1356
Bpu1102I GCTNAGC 1 cut(s) 1840
Bpu14I TTCGAA 1 cut(s) 584
BpuEI CTTGAG 3 cut(s) 332, 1093, 2456
BpuMI CCSGG 1 cut(s) 1184
BsaBI GATNNNNATC 1 cut(s) 2343
BsaI GGTCTC 2 cut(s) 606, 855
BsaJI CCNNGG 4 cut(s) 1208, 1654, 2074, 2147
BsaWI WCCGGW 1 cut(s) 2347
BsaXI ACNNNNNCTCC 4 cut(s) 1772, 1802, 2011, 2041
Bsc4I CCNNNNNNNGG 8 cut(s) 261, 562, 1405, 1467, 1545, 1829, 2059, 2461
Bse1I ACTGG 9 cut(s) 33, 316, 486, 799, 1039, 1834, 1946, 2172, 2323
Bse3DI GCAATG 1 cut(s) 1317
Bse8I GATNNNNATC 1 cut(s) 2343
BseAI TCCGGA 1 cut(s) 2347
BseBI CCWGG 3 cut(s) 1710, 1766, 2220
BseDI CCNNGG 4 cut(s) 1208, 1654, 2074, 2147
BseGI GGATG 2 cut(s) 1218, 1501
BseJI GATNNNNATC 1 cut(s) 2343
BseLI CCNNNNNNNGG 8 cut(s) 261, 562, 1405, 1467, 1545, 1829, 2059, 2461
BseMI GCAATG 1 cut(s) 1317
BseNI ACTGG 9 cut(s) 33, 316, 486, 799, 1039, 1834, 1946, 2172, 2323
BseRI GAGGAG 2 cut(s) 2366, 2477
BseSI GKGCMC 1 cut(s) 2492
BseXI GCAGC 5 cut(s) 610, 1298, 1364, 1759, 2035
BseYI CCCAGC 3 cut(s) 1330, 1844, 2376
Bsh1236I CGCG 1 cut(s) 1656
BshFI GGCC 4 cut(s) 414, 1397, 1515, 1687
BsiHKAI GWGCWC 3 cut(s) 1949, 2313, 2492
BsiHKCI CYCGRG 1 cut(s) 2052
BsiSI CCGG 2 cut(s) 1184, 2348
BslFI GGGAC 3 cut(s) 692, 1166, 1386
BslI CCNNNNNNNGG 8 cut(s) 261, 562, 1405, 1467, 1545, 1829, 2059, 2461
BsmAI GTCTC 5 cut(s) 97, 494, 606, 855, 1142
BsmFI GGGAC 3 cut(s) 692, 1166, 1386
BsmI GAATGC 3 cut(s) 531, 971, 1980
BsnI GGCC 4 cut(s) 414, 1397, 1515, 1687
Bso31I GGTCTC 2 cut(s) 606, 855
BsoBI CYCGRG 1 cut(s) 2052
Bsp119I TTCGAA 1 cut(s) 584
Bsp1286I GDGCHC 3 cut(s) 1949, 2313, 2492
Bsp13I TCCGGA 1 cut(s) 2347
Bsp143I GATC 4 cut(s) 574, 1084, 2202, 2422
Bsp1720I GCTNAGC 1 cut(s) 1840
Bsp19I CCATGG 1 cut(s) 2074
BspACI CCGC 4 cut(s) 1323, 1654, 1656, 1916
BspANI GGCC 4 cut(s) 414, 1397, 1515, 1687
BspEI TCCGGA 1 cut(s) 2347
BspFNI CGCG 1 cut(s) 1656
BspHI TCATGA 1 cut(s) 1906
BspLI GGNNCC 5 cut(s) 385, 1516, 1889, 2024, 2145
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 2552
BspOI GCTAGC 1 cut(s) 840
BspPI GGATC 1 cut(s) 2417
BspT104I TTCGAA 1 cut(s) 584
BspTNI GGTCTC 2 cut(s) 606, 855
BsrDI GCAATG 1 cut(s) 1317
BsrI ACTGG 9 cut(s) 33, 316, 486, 799, 1039, 1834, 1946, 2172, 2323
BssECI CCNNGG 4 cut(s) 1208, 1654, 2074, 2147
BssMI GATC 4 cut(s) 574, 1084, 2202, 2422
BssT1I CCWWGG 3 cut(s) 1208, 2074, 2147
Bst2UI CCWGG 3 cut(s) 1710, 1766, 2220
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 2 cut(s) 1211, 2193
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 5 cut(s) 838, 969, 1383, 1566, 2522
BstDSI CCRYGG 2 cut(s) 1654, 2074
BstEII GGTNACC 1 cut(s) 2564
BstF5I GGATG 2 cut(s) 1218, 1501
BstFNI CGCG 1 cut(s) 1656
BstH2I RGCGCY 1 cut(s) 2418
BstHHI GCGC 2 cut(s) 949, 2417
BstKTI GATC 4 cut(s) 577, 1087, 2205, 2425
BstMAI GTCTC 5 cut(s) 97, 494, 606, 855, 1142
BstMBI GATC 4 cut(s) 574, 1084, 2202, 2422
BstMWI GCNNNNNNNGC 6 cut(s) 274, 411, 1331, 2045, 2054, 2220
BstNI CCWGG 3 cut(s) 1710, 1766, 2220
BstNSI RCATGY 3 cut(s) 971, 1991, 2524
BstPI GGTNACC 1 cut(s) 2564
BstSCI CCNGG 4 cut(s) 1182, 1708, 1764, 2218
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 2492
BstUI CGCG 1 cut(s) 1656
BstV1I GCAGC 5 cut(s) 610, 1298, 1364, 1759, 2035
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 2202
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 1 cut(s) 2202
BsuRI GGCC 4 cut(s) 414, 1397, 1515, 1687
BtgI CCRYGG 2 cut(s) 1654, 2074
BtrI CACGTC 1 cut(s) 2447
BtsCI GGATG 2 cut(s) 1218, 1501
BtsI GCAGTG 1 cut(s) 1734
BtsIMutI CAGTG 4 cut(s) 38, 309, 753, 1734
BveI ACCTGC 1 cut(s) 2552
Cac8I GCNNGC 5 cut(s) 838, 969, 1383, 1566, 2522
CciI TCATGA 1 cut(s) 1906
CfoI GCGC 2 cut(s) 949, 2417
Cfr13I GGNCC 5 cut(s) 289, 384, 1396, 1514, 1685
Cfr42I CCGCGG 1 cut(s) 1657
Csp6I GTAC 2 cut(s) 824, 1077
CspCI CAANNNNNGTGG 2 cut(s) 1153, 1188
CviAII CATG 9 cut(s) 671, 968, 1721, 1745, 1876, 1907, 1988, 2075, 2521
CviQI GTAC 2 cut(s) 824, 1077
DpnI GATC 4 cut(s) 576, 1086, 2204, 2424
DpnII GATC 4 cut(s) 574, 1084, 2202, 2422
DraIII CACNNNGTG 1 cut(s) 1253
DriI GACNNNNNGTC 1 cut(s) 1630
Eam1104I CTCTTC 2 cut(s) 1211, 2193
Eam1105I GACNNNNNGTC 1 cut(s) 1630
EarI CTCTTC 2 cut(s) 1211, 2193
Ecl136II GAGCTC 1 cut(s) 2311
Eco130I CCWWGG 3 cut(s) 1208, 2074, 2147
Eco24I GRGCYC 1 cut(s) 2313
Eco31I GGTCTC 2 cut(s) 606, 855
Eco32I GATATC 1 cut(s) 2483
Eco47I GGWCC 2 cut(s) 289, 384
Eco47III AGCGCT 1 cut(s) 2416
Eco53kI GAGCTC 1 cut(s) 2311
Eco57I CTGAAG 5 cut(s) 522, 936, 1692, 1877, 2019
Eco88I CYCGRG 1 cut(s) 2052
Eco91I GGTNACC 1 cut(s) 2564
EcoICRI GAGCTC 1 cut(s) 2311
EcoO109I RGGNCCY 1 cut(s) 384
EcoO65I GGTNACC 1 cut(s) 2564
EcoRI GAATTC 1 cut(s) 1716
EcoRII CCWGG 3 cut(s) 1708, 1764, 2218
EcoRV GATATC 1 cut(s) 2483
EcoT14I CCWWGG 3 cut(s) 1208, 2074, 2147
EcoT22I ATGCAT 1 cut(s) 973
EcoT38I GRGCYC 1 cut(s) 2313
ErhI CCWWGG 3 cut(s) 1208, 2074, 2147
FaeI CATG 9 cut(s) 674, 971, 1724, 1748, 1879, 1910, 1991, 2078, 2524
FaqI GGGAC 3 cut(s) 692, 1166, 1386
FatI CATG 9 cut(s) 670, 967, 1720, 1744, 1875, 1906, 1987, 2074, 2520
Fnu4HI GCNGC 7 cut(s) 624, 1312, 1323, 1353, 1773, 1916, 2049
FokI GGATG 2 cut(s) 1225, 1508
FriOI GRGCYC 1 cut(s) 2313
Fsp4HI GCNGC 7 cut(s) 624, 1312, 1323, 1353, 1773, 1916, 2049
GlaI GCGC 2 cut(s) 948, 2416
GluI GCNGC 7 cut(s) 624, 1312, 1323, 1353, 1773, 1916, 2049
GsaI CCCAGC 3 cut(s) 1334, 1848, 2380
GsuI CTGGAG 2 cut(s) 192, 1963
HaeII RGCGCY 1 cut(s) 2418
HaeIII GGCC 4 cut(s) 414, 1397, 1515, 1687
HapII CCGG 2 cut(s) 1184, 2348
HhaI GCGC 2 cut(s) 949, 2417
Hin1II CATG 9 cut(s) 674, 971, 1724, 1748, 1879, 1910, 1991, 2078, 2524
Hin6I GCGC 2 cut(s) 947, 2415
HinP1I GCGC 2 cut(s) 947, 2415
HincII GTYRAC 2 cut(s) 65, 2010
HindII GTYRAC 2 cut(s) 65, 2010
HindIII AAGCTT 3 cut(s) 275, 424, 1379
HpaI GTTAAC 1 cut(s) 2010
HpaII CCGG 2 cut(s) 1184, 2348
HphI GGTGA 4 cut(s) 467, 790, 2076, 2348
Hpy166II GTNNAC 6 cut(s) 65, 1079, 1693, 2010, 2241, 2490
Hpy8I GTNNAC 6 cut(s) 65, 1079, 1693, 2010, 2241, 2490
HpyAV CCTTC 9 cut(s) 546, 800, 1346, 1446, 1534, 1667, 1901, 1994, 2510
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 1 cut(s) 2446
HpyCH4V TGCA 9 cut(s) 106, 467, 971, 1385, 1449, 1744, 1775, 2490, 2524
HpyF10VI GCNNNNNNNGC 6 cut(s) 274, 411, 1331, 2045, 2054, 2220
HpySE526I ACGT 1 cut(s) 2446
Hsp92II CATG 9 cut(s) 674, 971, 1724, 1748, 1879, 1910, 1991, 2078, 2524
HspAI GCGC 2 cut(s) 947, 2415
Kpn2I TCCGGA 1 cut(s) 2347
KspAI GTTAAC 1 cut(s) 2010
KspI CCGCGG 1 cut(s) 1657
Kzo9I GATC 4 cut(s) 574, 1084, 2202, 2422
LmnI GCTCC 6 cut(s) 157, 333, 766, 1849, 1944, 2143
Lsp1109I GCAGC 5 cut(s) 610, 1298, 1364, 1759, 2035
MaeII ACGT 1 cut(s) 2446
MaeIII GTNAC 4 cut(s) 440, 809, 2027, 2564
MalI GATC 4 cut(s) 576, 1086, 2204, 2424
MboI GATC 4 cut(s) 574, 1084, 2202, 2422
MflI RGATCY 1 cut(s) 2202
MhlI GDGCHC 3 cut(s) 1949, 2313, 2492
MlyI GAGTC 4 cut(s) 674, 1135, 1144, 1529
MmeI TCCRAC 4 cut(s) 753, 1176, 1997, 2515
Mph1103I ATGCAT 1 cut(s) 973
MroI TCCGGA 1 cut(s) 2347
MroXI GAANNNNTTC 4 cut(s) 734, 900, 990, 1220
MseI TTAA 8 cut(s) 357, 717, 761, 911, 1011, 1560, 2009, 2544
MslI CAYNNNNRTG 1 cut(s) 2103
MspA1I CMGCKG 3 cut(s) 1311, 1656, 2495
MspI CCGG 2 cut(s) 1184, 2348
MspR9I CCNGG 4 cut(s) 1184, 1710, 1766, 2220
Mva1269I GAATGC 3 cut(s) 531, 971, 1980
MvaI CCWGG 3 cut(s) 1710, 1766, 2220
MvnI CGCG 1 cut(s) 1656
MwoI GCNNNNNNNGC 6 cut(s) 274, 411, 1331, 2045, 2054, 2220
NciI CCSGG 1 cut(s) 1184
NcoI CCATGG 1 cut(s) 2074
NdeII GATC 4 cut(s) 574, 1084, 2202, 2422
NheI GCTAGC 1 cut(s) 836
NlaIII CATG 9 cut(s) 674, 971, 1724, 1748, 1879, 1910, 1991, 2078, 2524
NlaIV GGNNCC 5 cut(s) 385, 1516, 1889, 2024, 2145
NmeAIII GCCGAG 1 cut(s) 2190
NmuCI GTSAC 2 cut(s) 809, 2027
NsiI ATGCAT 1 cut(s) 973
NspI RCATGY 3 cut(s) 971, 1991, 2524
NspV TTCGAA 1 cut(s) 584
PaeI GCATGC 2 cut(s) 971, 2524
PagI TCATGA 1 cut(s) 1906
PciI ACATGT 1 cut(s) 1987
PctI GAATGC 3 cut(s) 531, 971, 1980
PdmI GAANNNNTTC 4 cut(s) 734, 900, 990, 1220
PflMI CCANNNNNTGG 4 cut(s) 562, 1467, 1545, 1829
PkrI GCNGC 7 cut(s) 625, 1313, 1324, 1354, 1774, 1917, 2050
PleI GAGTC 4 cut(s) 674, 1134, 1143, 1529
PpsI GAGTC 4 cut(s) 674, 1134, 1143, 1529
PpuMI RGGWCCY 1 cut(s) 384
PscI ACATGT 1 cut(s) 1987
PsiI TTATAA 1 cut(s) 2136
Psp124BI GAGCTC 1 cut(s) 2313
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 1708, 1764, 2218
PspEI GGTNACC 1 cut(s) 2564
PspFI CCCAGC 3 cut(s) 1330, 1844, 2376
PspGI CCWGG 3 cut(s) 1708, 1764, 2218
PspN4I GGNNCC 5 cut(s) 385, 1516, 1889, 2024, 2145
PspPI GGNCC 5 cut(s) 289, 384, 1396, 1514, 1685
PspPPI RGGWCCY 1 cut(s) 384
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 2202
PvuII CAGCTG 2 cut(s) 1311, 2495
RsaI GTAC 2 cut(s) 825, 1078
RsaNI GTAC 2 cut(s) 824, 1077
RseI CAYNNNNRTG 1 cut(s) 2103
SacI GAGCTC 1 cut(s) 2313
SacII CCGCGG 1 cut(s) 1657
SaqAI TTAA 8 cut(s) 357, 717, 761, 911, 1011, 1560, 2009, 2544
SatI GCNGC 7 cut(s) 624, 1312, 1323, 1353, 1773, 1916, 2049
Sau3AI GATC 4 cut(s) 574, 1084, 2202, 2422
Sau96I GGNCC 5 cut(s) 289, 384, 1396, 1514, 1685
SchI GAGTC 4 cut(s) 674, 1135, 1144, 1529
ScrFI CCNGG 4 cut(s) 1184, 1710, 1766, 2220
SduI GDGCHC 3 cut(s) 1949, 2313, 2492
SfcI CTRYAG 1 cut(s) 465
Sfr303I CCGCGG 1 cut(s) 1657
SfuI TTCGAA 1 cut(s) 584
SgrBI CCGCGG 1 cut(s) 1657
SinI GGWCC 2 cut(s) 289, 384
SmiMI CAYNNNNRTG 1 cut(s) 2103
SmlI CTYRAG 3 cut(s) 347, 1072, 2471
SmoI CTYRAG 3 cut(s) 347, 1072, 2471
SpeI ACTAGT 2 cut(s) 710, 1025
SphI GCATGC 2 cut(s) 971, 2524
SsiI CCGC 4 cut(s) 1323, 1654, 1656, 1916
SstI GAGCTC 1 cut(s) 2313
StyD4I CCNGG 4 cut(s) 1182, 1708, 1764, 2218
StyI CCWWGG 3 cut(s) 1208, 2074, 2147
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 1 cut(s) 2449
TaqI TCGA 4 cut(s) 584, 1019, 1593, 1798
TatI WGTACW 1 cut(s) 1076
TauI GCSGC 2 cut(s) 1325, 1918
Tru1I TTAA 8 cut(s) 357, 717, 761, 911, 1011, 1560, 2009, 2544
Tru9I TTAA 8 cut(s) 357, 717, 761, 911, 1011, 1560, 2009, 2544
TscAI CASTG 4 cut(s) 45, 316, 753, 1734
TseFI GTSAC 2 cut(s) 809, 2027
TseI GCWGC 5 cut(s) 623, 1311, 1352, 1772, 2048
Tsp45I GTSAC 2 cut(s) 809, 2027
TspGWI ACGGA 1 cut(s) 2015
TspRI CASTG 4 cut(s) 45, 316, 753, 1734
Van91I CCANNNNNTGG 4 cut(s) 562, 1467, 1545, 1829
VneI GTGCAC 1 cut(s) 2488
VpaK11BI GGWCC 2 cut(s) 289, 384
XapI RAATTY 5 cut(s) 1113, 1645, 1716, 1897, 2430
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 3 cut(s) 971, 1991, 2524
XcmI CCANNNNNNNNNTGG 2 cut(s) 327, 1546
XmnI GAANNNNTTC 4 cut(s) 734, 900, 990, 1220
Zsp2I ATGCAT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.