pycom15g13700

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016668
Physical Location & Seq
Reverse (-)
9286976 .. 9289558
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g13700.1

Sequence Viewer

Length: 2583 bp
ATGGGTTATATTTTCTTTGCTTCTCTATTAGTTTTTTCCCTGCTTTTTAATCCATTAGCTTCCCAACAGCCCAACACAGATGGGTTCTTTGTCTCTGAGTTTTTGAAAAAATTGGGTTCTTTTTCCTCATCTTCTTCTTCCAATCTCTACAACTTTTCTGCACCTGTTTGCTCGTGGAAAGGAGTTTTCTGTGAATCCAACAATGTTATCGGATTTGAGGCTTCCGGTTTGGAGCTCTCCGGTCCGATTCCGGACACAACCATCGGAAAGCTCACAAAGCTCCAATCTTTAAACCTTAGCAGCAACAAAATCACCAGTCTGCCTTCGGATTTGTGGAGCCTAGGTTCTCTCAAGCACCTTATTCTCTCCAACAACCGGATTTCCGGGTCGTTGCCTAACAACGTTGGCAACTTCGGTCTGCTCGAAACCCTCGACGTTTCCAACAACAATTTCTCTGGTGAGATTCCTGCAGATATAAGCTCACTTGCCAGTCTGCGAGTTCTTAAGCTCAATGGAAATTCGTTTGAGAAGAGCATCCCATCAGGAATTTTGAGTTGTCAGTCTCTGGTTTGGATTGACCTTTCGTCGAATCGGTTAAACGGTTCTCTTCCAGATGGATTTGGTGCTGCATTGCCTAAGCTCCAAGTATTGAACCTTGCTGGGAATGGGATTCAAGGCCGGGACTCCGATTTTTCGGAGATGAATTCCATTACTTCTCTGAACATTTCAGGAAATGTGTTTCAGGGTTCAGTGATGGGAGTGTTTAAGGAGCTGCTGGAGGTGGCAGACCTGAGCAGGAATCGGTTTGAAGGCCACGTTTCAAAGGTACAATTCAATGCTAGCTACAATTGGTCTCGTTTGGTTTATTTAGATTTGTCTGAGAATCAGCTTGGTGGAGAAATTTTACTTGATTGGATTCAATCCCAGAATCTCAAATACCTTAATCTAGCCCACAATAATTTTACAAGGCAGGAATTCCGGAGAAATGTGAAAACTTTTTTGGGTTTGGAGTACCTGAACTTGTCGAAAACTAGTCTTACGGGTCGTATTCCTGCTGAAATTTCCCAGCTGAGTAACTTGAACACACTTGATCTTTCTGAAAACCATCTCATAGGCAGAATCCCATTGCTGAGTTTGAGAAATCTCCAAGTTTTTGATGTTTCTAACAACAATTTAAGCGGAGAGATCCCTGCATCGCTCTTGGGGAAACTCCCTTGGATGGAGAGGTTCAACTTCTCTTACAATAACTTGACACTTTGTGCTTCTGAAATTTCCAAAGCAACCCTCCAAGCATCTTTCTTTGGTTCAACAAACAGCTGCCCCATTGCAGCAAAACCAGACCTTTTTAGAGCACGATCCAATAAGGCTAGGCAGCAAAAGGGACTAAAGCTTGCTTTGGTTTTGACCTTCTCAATGGTATGCCTGCTCGCTGGTTTGCTGTTTCTAGCAGTTGGTTGCAGAAGGAAAACCAGAATGTGGGAGCTAAAACAAACCTCACACAAGGAAGAGCAAAATGTATCAGGCCCCTTTTCCTTCCAGACCGATTCAACAACATGGGTTGCGGATGTTAAGCAAGCAAATTCAGTACCTGTAGTGATTTTCGGGAAGCCATTGTTGAATTTCACATTCTCAGACCTCTTATCTGCAACTTCGAACTTTGATCGAGGAACCCTTTTGGCTGAAGGGAAATTTGGGCCAGTTTATAGAGGATTTCTACCGGGTGGAATCCATGTAGCAGTGAAAGTTTTGGTCCATGGTTCTACAATGACAGACCAGGAAGCTGCAAGGGAGTTTGAGTATCTCGGTCGAATCAAACACCCTAATCTTGCTCCATTGACTGGATATTGCTTGGCTGGGGACCAAAGGATTGCTATCTATGACTACATGGAGAACGGGAATCTACAGAATTTGCTTCATGACTTGCCGCTCGGGGTACTGCAAACAACAGAGGATTGGAGCACAGATACTTGGGAACAGGAAGACAACAATGACATTCAAAATGTTGGTTCTGAAGGGTTGTTAACATCGTGGAGGTTCCGTCACAAGATTGCACTTGGCACTGCCAGAGCACTGGCATTTCTACACCACGGTTGCTCACCTCCGATTATTCATAGAGACGTTAAAGCTAGTAGTGTCTATCTGGATTATAACTTAGAGCCTAGATTATCTGATTTTGGTCTGGCTAAGATTTTTGGCAGTGGCCTAGATGAAGAGATCTCTCAAGGGTCACCAGGGTATGTGCCACCAGAGTTTTCTCAGTCCGAGTATGATTCTCCAACACCAAAATCTGATGTGTATTGCTTTGGAGTTGTTCTTTTTGAGTTGATTACTTCGAAGAAGCCATTTGGCGATGACTATCCAGAGGAGAAGGATGCAACCTTGGTGAGCTGGGTTAGAGGATTGATTAGAAAGAACAAAGGGTCGAGCGCTATTGATCCAAAAATTCGTGACACAGGACCAGATGACCAAATGGAGGAGGCCCTGAAGATTGGATATCTGTGCACAGCTGACCTTCCCTCAAAGCGACCAAGCATGCATCAGATTGTCGGACTTCTTAAAGATATCGAGCAAACAGGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

861

Amino Acids

94.73

Weight (kDa)

5.95

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 83 - 127 5.1e-09 Leucine rich repeat
LRR_14 PF23598 87 - 179 6e-11 Leucine-rich repeat region
LRR_8 PF13855 188 - 245 8.6e-08 Leucine rich repeat
LRR_14 PF23598 309 - 409 3.2e-06 Leucine-rich repeat region
Pkinase PF00069 556 - 849 2.3e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 557 - 850 1e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016668)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24230
fragaria_vesca FvH4_1g01000
malus_domestica MD02G1008100.v1.1 MD15G1152400.v1.1
prunus_persica Prupe.7G262300_v2.0.a1
pyrus_communis pycom15g13700
rosa_chinensis RchiOBHm_Chr2g0085731
rosa_laevigata RLG00000015715
rosa_multiflora Rmu_sc0009759.1_g000005 Rmu_sc0023131.1_g000002 Rmu_sc0033740.1_g000001
rosa_rugosa Rorug01G0462800
rosa_samantha Rh2CG013300
rosa_wichuraiana Rw2G001060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2148
AccB7I CCANNNNNTGG 2 cut(s) 1476, 1838
AccBSI CCGCTC 1 cut(s) 1927
AccIII TCCGGA 2 cut(s) 250, 978
AciI CCGC 3 cut(s) 1179, 1562, 1925
AclI AACGTT 1 cut(s) 402
AclWI GGATC 3 cut(s) 1180, 1350, 2429
AcuI CTGAAG 3 cut(s) 1701, 2031, 2504
AdeI CACNNNGTG 1 cut(s) 1259
AfaI GTAC 4 cut(s) 828, 1013, 1587, 1935
AfeI AGCGCT 1 cut(s) 2428
AfiI CCNNNNNNNGG 5 cut(s) 375, 1219, 1476, 1838, 2473
AflII CTTAAG 1 cut(s) 503
AhdI GACNNNNNGTC 1 cut(s) 583
AhlI ACTAGT 1 cut(s) 1031
AjnI CCWGG 2 cut(s) 1773, 2230
AjuI GAANNNNNNNTTGG 6 cut(s) 434, 466, 983, 1015, 1674, 1706
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
Alw21I GWGCWC 5 cut(s) 237, 1354, 1961, 2071, 2504
Alw26I GTCTC 4 cut(s) 97, 567, 858, 2109
Alw44I GTGCAC 1 cut(s) 2500
AlwI GGATC 3 cut(s) 1180, 1350, 2429
AlwNI CAGNNNCTG 2 cut(s) 565, 1589
Ama87I CYCGRG 1 cut(s) 1928
Aor13HI TCCGGA 2 cut(s) 250, 978
Aor51HI AGCGCT 1 cut(s) 2428
AoxI GGCC 6 cut(s) 676, 811, 1522, 1694, 2200, 2478
ApaLI GTGCAC 1 cut(s) 2500
ApeKI GCWGC 7 cut(s) 300, 626, 772, 1317, 1328, 1372, 1781
AspA2I CCTAGG 1 cut(s) 340
AspLEI GCGC 1 cut(s) 2429
AspS9I GGNCC 7 cut(s) 242, 1523, 1694, 1750, 1858, 2456, 2479
AsuC2I CCSGG 3 cut(s) 385, 680, 1719
AsuHPI GGTGA 5 cut(s) 304, 470, 2088, 2220, 2395
AsuII TTCGAA 2 cut(s) 1652, 2333
AsuNHI GCTAGC 1 cut(s) 839
AvaI CYCGRG 1 cut(s) 1928
AvaII GGWCC 4 cut(s) 242, 1750, 1858, 2456
AvrII CCTAGG 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 2504
BanII GRGCYC 1 cut(s) 237
BauI CACGAG 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 1986
Bbv12I GWGCWC 5 cut(s) 237, 1354, 1961, 2071, 2504
BbvI GCAGC 7 cut(s) 312, 613, 759, 1304, 1340, 1384, 1768
BccI CCATC 7 cut(s) 74, 269, 547, 608, 748, 1113, 1213
BciT130I CCWGG 2 cut(s) 1775, 2232
BcnI CCSGG 3 cut(s) 385, 680, 1719
BcoDI GTCTC 4 cut(s) 97, 567, 858, 2109
BcuI ACTAGT 1 cut(s) 1031
BfaI CTAG 9 cut(s) 341, 840, 947, 1032, 1368, 1445, 2127, 2160, 2204
BfmI CTRYAG 3 cut(s) 468, 1590, 1901
BfoI RGCGCY 1 cut(s) 2430
BfrI CTTAAG 1 cut(s) 503
BglII AGATCT 1 cut(s) 2214
BisI GCNGC 8 cut(s) 301, 627, 773, 1318, 1329, 1373, 1782, 1925
BlnI CCTAGG 1 cut(s) 340
BlsI GCNGC 8 cut(s) 302, 628, 774, 1319, 1330, 1374, 1783, 1926
Bme1390I CCNGG 5 cut(s) 385, 680, 1719, 1775, 2232
Bme18I GGWCC 4 cut(s) 242, 1750, 1858, 2456
BmeRI GACNNNNNGTC 1 cut(s) 583
BmeT110I CYCGRG 1 cut(s) 1928
BmgT120I GGNCC 7 cut(s) 242, 1523, 1694, 1750, 1858, 2456, 2479
BmiI GGNNCC 5 cut(s) 338, 1525, 1669, 1859, 2036
BmrFI CCNGG 5 cut(s) 385, 680, 1719, 1775, 2232
BmsI GCATC 5 cut(s) 543, 1202, 1301, 2362, 2545
BmtI GCTAGC 1 cut(s) 843
BpiI GAAGAC 1 cut(s) 1986
BpmI CTGGAG 1 cut(s) 797
Bpu10I CCTNAGC 3 cut(s) 296, 636, 791
Bpu14I TTCGAA 2 cut(s) 1652, 2333
BpuEI CTTGAG 2 cut(s) 335, 2205
BpuMI CCSGG 3 cut(s) 385, 680, 1719
BsaBI GATNNNNATC 2 cut(s) 1871, 2355
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 6 cut(s) 340, 1214, 1753, 2086, 2231, 2379
BsaWI WCCGGW 5 cut(s) 224, 239, 250, 375, 978
BsaXI ACNNNNNCTCC 6 cut(s) 328, 358, 1781, 1811, 2023, 2053
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 1219, 1476, 1838, 2473
Bse1I ACTGG 5 cut(s) 315, 489, 1697, 1843, 2076
Bse3DI GCAATG 3 cut(s) 629, 1124, 1323
Bse8I GATNNNNATC 2 cut(s) 1871, 2355
BseAI TCCGGA 2 cut(s) 250, 978
BseBI CCWGG 2 cut(s) 1775, 2232
BseDI CCNNGG 6 cut(s) 340, 1214, 1753, 2086, 2231, 2379
BseGI GGATG 4 cut(s) 534, 1224, 1570, 2377
BseJI GATNNNNATC 2 cut(s) 1871, 2355
BseLI CCNNNNNNNGG 5 cut(s) 375, 1219, 1476, 1838, 2473
BseMI GCAATG 3 cut(s) 629, 1124, 1323
BseMII CTCAG 7 cut(s) 87, 782, 870, 1061, 1121, 1644, 2270
BseNI ACTGG 5 cut(s) 315, 489, 1697, 1843, 2076
BseRI GAGGAG 2 cut(s) 2378, 2489
BseSI GKGCMC 1 cut(s) 2504
BseXI GCAGC 7 cut(s) 312, 613, 759, 1304, 1340, 1384, 1768
BseYI CCCAGC 4 cut(s) 659, 1065, 1853, 2388
BsgI GTGCAG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 1807
BshFI GGCC 6 cut(s) 678, 813, 1524, 1696, 2202, 2480
BsiEI CGRYCG 1 cut(s) 1807
BsiHKAI GWGCWC 5 cut(s) 237, 1354, 1961, 2071, 2504
BsiHKCI CYCGRG 1 cut(s) 1928
BsiSI CCGG 8 cut(s) 225, 240, 251, 376, 384, 679, 979, 1718
BslFI GGGAC 3 cut(s) 695, 1395, 1871
BslI CCNNNNNNNGG 5 cut(s) 375, 1219, 1476, 1838, 2473
BsmAI GTCTC 4 cut(s) 97, 567, 858, 2109
BsmBI CGTCTC 1 cut(s) 2109
BsmFI GGGAC 3 cut(s) 695, 1395, 1871
BsnI GGCC 6 cut(s) 678, 813, 1524, 1696, 2202, 2480
Bso31I GGTCTC 1 cut(s) 858
BsoBI CYCGRG 1 cut(s) 1928
Bsp119I TTCGAA 2 cut(s) 1652, 2333
Bsp1286I GDGCHC 5 cut(s) 237, 1354, 1961, 2071, 2504
Bsp13I TCCGGA 2 cut(s) 250, 978
Bsp143I GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
Bsp19I CCATGG 1 cut(s) 1753
BspACI CCGC 3 cut(s) 1179, 1562, 1925
BspANI GGCC 6 cut(s) 678, 813, 1524, 1696, 2202, 2480
BspCNI CTCAG 7 cut(s) 88, 783, 871, 1062, 1122, 1643, 2269
BspEI TCCGGA 2 cut(s) 250, 978
BspHI TCATGA 1 cut(s) 1915
BspLI GGNNCC 5 cut(s) 338, 1525, 1669, 1859, 2036
BspMAI CTGCAG 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 843
BspPI GGATC 3 cut(s) 1180, 1350, 2429
BspQI GCTCTTC 2 cut(s) 524, 1500
BspT104I TTCGAA 2 cut(s) 1652, 2333
BspTI CTTAAG 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 858
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 3 cut(s) 629, 1124, 1323
BsrI ACTGG 5 cut(s) 315, 489, 1697, 1843, 2076
BssECI CCNNGG 6 cut(s) 340, 1214, 1753, 2086, 2231, 2379
BssMI GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
BssSI CACGAG 1 cut(s) 172
BssT1I CCWWGG 4 cut(s) 340, 1214, 1753, 2379
Bst2BI CACGAG 1 cut(s) 172
Bst2UI CCWGG 2 cut(s) 1775, 2232
Bst4CI ACNGT 2 cut(s) 602, 2090
Bst6I CTCTTC 4 cut(s) 524, 612, 1500, 2205
BstAFI CTTAAG 1 cut(s) 503
BstBI TTCGAA 2 cut(s) 1652, 2333
BstC8I GCNNGC 6 cut(s) 841, 1392, 1424, 1428, 1575, 2534
BstDSI CCRYGG 2 cut(s) 1753, 2086
BstEII GGTNACC 1 cut(s) 2226
BstF5I GGATG 4 cut(s) 534, 1224, 1570, 2377
BstH2I RGCGCY 1 cut(s) 2430
BstHHI GCGC 1 cut(s) 2429
BstKTI GATC 6 cut(s) 1093, 1188, 1358, 1663, 2217, 2437
BstMAI GTCTC 4 cut(s) 97, 567, 858, 2109
BstMBI GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
BstMCI CGRYCG 1 cut(s) 1807
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstNI CCWGG 2 cut(s) 1775, 2232
BstNSI RCATGY 1 cut(s) 2536
BstPI GGTNACC 1 cut(s) 2226
BstSCI CCNGG 5 cut(s) 383, 678, 1717, 1773, 2230
BstSFI CTRYAG 3 cut(s) 468, 1590, 1901
BstSLI GKGCMC 1 cut(s) 2504
BstV1I GCAGC 7 cut(s) 312, 613, 759, 1304, 1340, 1384, 1768
BstV2I GAAGAC 1 cut(s) 1986
BstX2I RGATCY 2 cut(s) 1185, 2214
BstXI CCANNNNNNTGG 1 cut(s) 2071
BstYI RGATCY 2 cut(s) 1185, 2214
BsuRI GGCC 6 cut(s) 678, 813, 1524, 1696, 2202, 2480
BtgI CCRYGG 2 cut(s) 1753, 2086
BtgZI GCGATG 2 cut(s) 1179, 2364
BtsCI GGATG 4 cut(s) 534, 1224, 1570, 2377
BtsI GCAGTG 3 cut(s) 1743, 2058, 2203
BtsIMutI CAGTG 5 cut(s) 756, 1743, 2058, 2069, 2203
Cac8I GCNNGC 6 cut(s) 841, 1392, 1424, 1428, 1575, 2534
CaiI CAGNNNCTG 2 cut(s) 565, 1589
CciI TCATGA 1 cut(s) 1915
CfoI GCGC 1 cut(s) 2429
Cfr13I GGNCC 7 cut(s) 242, 1523, 1694, 1750, 1858, 2456, 2479
CpoI CGGWCCG 1 cut(s) 242
Csp6I GTAC 4 cut(s) 827, 1012, 1586, 1934
CspI CGGWCCG 1 cut(s) 242
CviAII CATG 6 cut(s) 1554, 1730, 1754, 1885, 1916, 2533
CviQI GTAC 4 cut(s) 827, 1012, 1586, 1934
DpnI GATC 6 cut(s) 1092, 1187, 1357, 1662, 2216, 2436
DpnII GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
DraI TTTAAA 1 cut(s) 291
DraIII CACNNNGTG 1 cut(s) 1259
DriI GACNNNNNGTC 1 cut(s) 583
Eam1104I CTCTTC 4 cut(s) 524, 612, 1500, 2205
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 4 cut(s) 524, 612, 1500, 2205
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 4 cut(s) 340, 1214, 1753, 2379
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 858
Eco32I GATATC 2 cut(s) 2495, 2563
Eco47I GGWCC 4 cut(s) 242, 1750, 1858, 2456
Eco47III AGCGCT 1 cut(s) 2428
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 3 cut(s) 1701, 2031, 2504
Eco88I CYCGRG 1 cut(s) 1928
Eco91I GGTNACC 1 cut(s) 2226
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 2 cut(s) 1523, 2479
EcoO65I GGTNACC 1 cut(s) 2226
EcoRI GAATTC 2 cut(s) 703, 974
EcoRII CCWGG 2 cut(s) 1773, 2230
EcoRV GATATC 2 cut(s) 2495, 2563
EcoT14I CCWWGG 4 cut(s) 340, 1214, 1753, 2379
EcoT22I ATGCAT 1 cut(s) 2538
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 4 cut(s) 340, 1214, 1753, 2379
Esp3I CGTCTC 1 cut(s) 2109
FaeI CATG 6 cut(s) 1557, 1733, 1757, 1888, 1919, 2536
FaqI GGGAC 3 cut(s) 695, 1395, 1871
FatI CATG 6 cut(s) 1553, 1729, 1753, 1884, 1915, 2532
Fnu4HI GCNGC 8 cut(s) 301, 627, 773, 1318, 1329, 1373, 1782, 1925
FokI GGATG 4 cut(s) 521, 1231, 1577, 2384
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 8 cut(s) 301, 627, 773, 1318, 1329, 1373, 1782, 1925
FspBI CTAG 9 cut(s) 341, 840, 947, 1032, 1368, 1445, 2127, 2160, 2204
GlaI GCGC 1 cut(s) 2428
GluI GCNGC 8 cut(s) 301, 627, 773, 1318, 1329, 1373, 1782, 1925
GsaI CCCAGC 4 cut(s) 663, 1069, 1857, 2392
GsuI CTGGAG 1 cut(s) 797
HaeII RGCGCY 1 cut(s) 2430
HaeIII GGCC 6 cut(s) 678, 813, 1524, 1696, 2202, 2480
HapII CCGG 8 cut(s) 225, 240, 251, 376, 384, 679, 979, 1718
HhaI GCGC 1 cut(s) 2429
Hin1II CATG 6 cut(s) 1557, 1733, 1757, 1888, 1919, 2536
Hin6I GCGC 1 cut(s) 2427
HinP1I GCGC 1 cut(s) 2427
HincII GTYRAC 1 cut(s) 2022
HindII GTYRAC 1 cut(s) 2022
HindIII AAGCTT 1 cut(s) 1388
HpaI GTTAAC 1 cut(s) 2022
HpaII CCGG 8 cut(s) 225, 240, 251, 376, 384, 679, 979, 1718
HphI GGTGA 5 cut(s) 304, 470, 2088, 2220, 2395
Hpy166II GTNNAC 2 cut(s) 2022, 2502
Hpy8I GTNNAC 2 cut(s) 2022, 2502
Hpy99I CGWCG 2 cut(s) 437, 589
HpyAV CCTTC 9 cut(s) 333, 803, 1417, 1455, 1543, 1676, 2006, 2362, 2522
HpyCH4III ACNGT 2 cut(s) 602, 2090
HpyCH4IV ACGT 4 cut(s) 402, 435, 816, 2118
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpySE526I ACGT 4 cut(s) 402, 435, 816, 2118
Hsp92II CATG 6 cut(s) 1557, 1733, 1757, 1888, 1919, 2536
HspAI GCGC 1 cut(s) 2427
Kpn2I TCCGGA 2 cut(s) 250, 978
KspAI GTTAAC 1 cut(s) 2022
Kzo9I GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
LguI GCTCTTC 2 cut(s) 524, 1500
LmnI GCTCC 8 cut(s) 232, 285, 336, 645, 769, 1480, 1834, 1956
Lsp1109I GCAGC 7 cut(s) 312, 613, 759, 1304, 1340, 1384, 1768
LweI GCATC 5 cut(s) 543, 1202, 1301, 2362, 2545
MaeI CTAG 9 cut(s) 341, 840, 947, 1032, 1368, 1445, 2127, 2160, 2204
MaeII ACGT 4 cut(s) 402, 435, 816, 2118
MaeIII GTNAC 5 cut(s) 1073, 2039, 2226, 2447, 2576
MalI GATC 6 cut(s) 1092, 1187, 1357, 1662, 2216, 2436
MbiI CCGCTC 1 cut(s) 1927
MboI GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
MfeI CAATTG 1 cut(s) 847
MflI RGATCY 2 cut(s) 1185, 2214
MhlI GDGCHC 5 cut(s) 237, 1354, 1961, 2071, 2504
MlyI GAGTC 1 cut(s) 677
MmeI TCCRAC 5 cut(s) 222, 393, 465, 2300, 2527
Mph1103I ATGCAT 1 cut(s) 2538
MroI TCCGGA 2 cut(s) 250, 978
MspA1I CMGCKG 3 cut(s) 1069, 1317, 2507
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 8 cut(s) 225, 240, 251, 376, 384, 679, 979, 1718
MspR9I CCNGG 5 cut(s) 385, 680, 1719, 1775, 2232
MunI CAATTG 1 cut(s) 847
MvaI CCWGG 2 cut(s) 1775, 2232
MwoI GCNNNNNNNGC 1 cut(s) 277
NciI CCSGG 3 cut(s) 385, 680, 1719
NcoI CCATGG 1 cut(s) 1753
NdeII GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
NheI GCTAGC 1 cut(s) 839
NlaIII CATG 6 cut(s) 1557, 1733, 1757, 1888, 1919, 2536
NlaIV GGNNCC 5 cut(s) 338, 1525, 1669, 1859, 2036
NmuCI GTSAC 3 cut(s) 2039, 2226, 2447
NsiI ATGCAT 1 cut(s) 2538
NspI RCATGY 1 cut(s) 2536
NspV TTCGAA 2 cut(s) 1652, 2333
PaeI GCATGC 1 cut(s) 2536
PagI TCATGA 1 cut(s) 1915
PciSI GCTCTTC 2 cut(s) 524, 1500
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 429
PflMI CCANNNNNTGG 2 cut(s) 1476, 1838
PkrI GCNGC 8 cut(s) 302, 628, 774, 1319, 1330, 1374, 1783, 1926
PleI GAGTC 1 cut(s) 677
PpsI GAGTC 1 cut(s) 677
PsiI TTATAA 1 cut(s) 2148
Psp124BI GAGCTC 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 1773, 2230
PspEI GGTNACC 1 cut(s) 2226
PspFI CCCAGC 4 cut(s) 659, 1065, 1853, 2388
PspGI CCWGG 2 cut(s) 1773, 2230
PspN4I GGNNCC 5 cut(s) 338, 1525, 1669, 1859, 2036
PspPI GGNCC 7 cut(s) 242, 1523, 1694, 1750, 1858, 2456, 2479
PstI CTGCAG 1 cut(s) 472
PstNI CAGNNNCTG 2 cut(s) 565, 1589
PsuI RGATCY 2 cut(s) 1185, 2214
PvuII CAGCTG 3 cut(s) 1069, 1317, 2507
RsaI GTAC 4 cut(s) 828, 1013, 1587, 1935
RsaNI GTAC 4 cut(s) 827, 1012, 1586, 1934
Rsr2I CGGWCCG 1 cut(s) 242
RsrII CGGWCCG 1 cut(s) 242
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 2 cut(s) 524, 1500
SatI GCNGC 8 cut(s) 301, 627, 773, 1318, 1329, 1373, 1782, 1925
Sau3AI GATC 6 cut(s) 1090, 1185, 1355, 1660, 2214, 2434
Sau96I GGNCC 7 cut(s) 242, 1523, 1694, 1750, 1858, 2456, 2479
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 5 cut(s) 385, 680, 1719, 1775, 2232
SduI GDGCHC 5 cut(s) 237, 1354, 1961, 2071, 2504
SfaNI GCATC 5 cut(s) 543, 1202, 1301, 2362, 2545
SfcI CTRYAG 3 cut(s) 468, 1590, 1901
SfuI TTCGAA 2 cut(s) 1652, 2333
SinI GGWCC 4 cut(s) 242, 1750, 1858, 2456
SmlI CTYRAG 3 cut(s) 350, 503, 2220
SmoI CTYRAG 3 cut(s) 350, 503, 2220
SpeI ACTAGT 1 cut(s) 1031
SphI GCATGC 1 cut(s) 2536
SsiI CCGC 3 cut(s) 1179, 1562, 1925
SspMI CTAG 9 cut(s) 341, 840, 947, 1032, 1368, 1445, 2127, 2160, 2204
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 5 cut(s) 383, 678, 1717, 1773, 2230
StyI CCWWGG 4 cut(s) 340, 1214, 1753, 2379
TaaI ACNGT 2 cut(s) 602, 2090
TaiI ACGT 4 cut(s) 405, 438, 819, 2121
TaqII GACCGA 3 cut(s) 404, 1556, 1793
TauI GCSGC 1 cut(s) 1927
TscAI CASTG 5 cut(s) 756, 1743, 2065, 2076, 2203
TseFI GTSAC 3 cut(s) 2039, 2226, 2447
TseI GCWGC 7 cut(s) 300, 626, 772, 1317, 1328, 1372, 1781
Tsp45I GTSAC 3 cut(s) 2039, 2226, 2447
TspDTI ATGAA 4 cut(s) 716, 1904, 2099, 2223
TspGWI ACGGA 1 cut(s) 2027
TspRI CASTG 5 cut(s) 756, 1743, 2065, 2076, 2203
Van91I CCANNNNNTGG 2 cut(s) 1476, 1838
Vha464I CTTAAG 1 cut(s) 503
VneI GTGCAC 1 cut(s) 2500
VpaK11BI GGWCC 4 cut(s) 242, 1750, 1858, 2456
XceI RCATGY 1 cut(s) 2536
XmaJI CCTAGG 1 cut(s) 340
XspI CTAG 9 cut(s) 341, 840, 947, 1032, 1368, 1445, 2127, 2160, 2204
Zsp2I ATGCAT 1 cut(s) 2538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.