MD02G1046100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008826
Physical Location & Seq
Forward (+)
3685303 .. 3689183
3881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1046100.v1.1.491

Sequence Viewer

Length: 2325 bp
ATGGTAGTCCCAACGCCGCAGCCGGCCGAGCACGCCGCCATGCCGCTCCCCGAAGCAAAGCCGAAAAAGAAGAGGTGGGAGAAGAGGGAAAGGAAGAACCCAATCGCAGTCTCTCTTGATGCTGACGGAAACCCAATTATTGATTCCCTAAATCCTGTAGAAATAATAGTCCCAACGCCGCAGCCGGCAGAGCACGCTGCCGTGCCGCTCCCAGAAGAAGCTCCGAAAAAGAAGAAGTGGGAGAAGAGGAAAAGGAAGAACCCAATCGTAGTGTCTCTTGATGCTGACGGAAACCCAATTGTCGATTCTAAAAAAATTCTTGAAAAAATAGTCCCAGCGCCACAGCCGGCAGAGCACGAAGCTGCGCTGCTCCCGGAAGCAGAGCTGAAAAAGAAGAGGTGGGAGAAGAAGAAAAAGAAGAACCCAACCGTAGTATCTCTTGATGCTGACGGAAACCCAATTGTCGATTCCCAAAAATCTGTTGAAATAATAGTCCCAGCGCCGCAGCCGGCAAAGCACGAAGCTGCGCTGCTCCCGGAAGCAGAGCCGAAAAAGAAGAGGTGGGAGAAGAAGAAAAAGAAGAACCCAATCGTAGTGTCTCTTGATGCTGACGGAAACCCAATTGACGATTCTCAAAAATCTGTTGAAATAATAGCCCCAGCGCCGCAACCGGCAGAGCACGAAGCTGCGCTGCTCCCGGAAGCAGAGCCGAAAAAGAAGAGGTGGGAGAAGAAGAAAAAGAAGAACCCAATCGTAGTGTCTCTTGATGCTGACGGAAACCCAATTGTCGATTCTCAAAAATCTGTTGAAATAATAGTCCCAGCACCGCAACAGGCAGAGCACGAAGCTGCGCTGCTCCCGGAAGCAGAGCCGAAAAAGAAGAGGTGGGAGAAGAAGAAAAAGAAGAACCCAATCGTAGTGTCTCTTGATGCTGACGGAAACCCAATTGTCGATTCTCAAAAATCTGTTGAAATAATAGTCCCAGCACCGCAACAGGCAGAGCACGAAGCTGCGCTGCTCCCGGAAGCAGAGCCGAAAAAGAAGAAGAAAAAGAAGAACCCAATCGTGGAGACTGATGGAAACCCAATTGTCGATTCCCAAAACTCTGTTGACATAAAGGAACAAGCGTTACAGCCAGAGCCAGAGCCTATAGGCCCAACTGCAGTCCCGCTTGAGGTAGAATTGGAAAACCCAGTCGTTGATTTGCGCAAATCGATTGAACAAACAGTCCCGAGCAATGAGAAGGCAATCACAGTCCCTGTTGAGGTGGGATTGGGGAATCCAGTTGTTAATGCTGCGAATTTTGAAGCAATAGTCCCAAGCAAGAAGAGCAAGAGAAAGAAGAGCAAAACCACAGTCCCAGGATTGGAAAATCCAGTTGCTGATGCGCCAGAAGCAGAGCAGACGGGACTGATTCCGAAGCCTGAGTCGATAGTTCCAGTAACAGTCCCGAGCACAACCACAGTCCCTGTCGAGGTGGGATTGGAAAACCTAGTTGTTGATGCGCCAAAATCTGTTGAAGCAATAGTCTGTAGCGCAACAGTTCCGAGCAAGAAAAGGAAGATAAAGAAGAGCAAAACCACAGTCCCTTTTGAGGTAGGATTGGAAAACCCAGTTGCTCATGCACCACAATCTATTGAGGTGGAGCAGGCGGGACTGGTTCCGAATCCTGAGCCGATAGTTTCAATGACAGTCCCGAGCACAACCATAGTCCATGTCGAGGGCGGATTGGAAAACCCAGTTGATTTGCCAAAATGTATTGAAGGAGAGCATGCTGGAGTGCTTCCGAAGCCTGAGCCAGTAGTCCCATCAACAATGCCGAGCAAGAAGAGGAAGAGAAAGAAGAGCACAGATAATGTCCCTGTTGATGTGGGATTGGAAAACACAGTTGTTGATGCGCCAAAACCTATTGAAGTAGACCAGGTGGGACTGTTTCAGAATCTGGAGCCGATAGTCCCACTGACAGTCCCGAGCACAACCACAATCCCTGTCGCGGTGCGATTGATAAACCCAGTTTTTGATGCGCCAAATTTTTTTAACGGAATTGTCTGGACCCCACAATGTGGAAGCTCGAGAATATATGAGAACAATAATAACACTTCCACATTCCCTATCGATGTGGAAGCTCGAGGATATGTTGAGATATGTGTCCTATGTCGGAAAAAAGGGCATAGCATCCCGACTTGCCCTGAGCTGAACAACCTCCATGTGCGTAGTGGAGTCCCGAAACTTGAAATGGAAATGAGAGAGAAGTTACCACCGATGCCTTGGGGTAGACGGAAGAGGAAGAAAAGTAGTGGTGGTAAAGAAGTCGGTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

775

Amino Acids

84.33

Weight (kDa)

8.74

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1208
AccB7I CCANNNNNTGG 1 cut(s) 2063
AccBSI CCGCTC 2 cut(s) 46, 208
AccI GTMKAC 2 cut(s) 1917, 2275
AccII CGCG 1 cut(s) 1994
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 3 cut(s) 315, 1300, 2029
AdeI CACNNNGTG 1 cut(s) 2063
AfiI CCNNNNNNNGG 9 cut(s) 1066, 1174, 1264, 1366, 1474, 1594, 1720, 1993, 2063
AjnI CCWGG 2 cut(s) 1360, 1920
AloI GAACNNNNNNTCC 2 cut(s) 1212, 1244
Alw26I GTCTC 6 cut(s) 115, 279, 603, 765, 927, 1064
AlwNI CAGNNNCTG 4 cut(s) 1259, 1382, 1469, 1942
Ama87I CYCGRG 6 cut(s) 1231, 1450, 1696, 1969, 2071, 2127
AoxI GGCC 2 cut(s) 24, 1153
ApoI RAATTY 3 cut(s) 315, 1300, 2029
ArsI GACNNNNNNTTYG 6 cut(s) 1341, 1373, 1412, 1444, 1569, 1601
AspS9I GGNCC 3 cut(s) 1154, 2052, 2315
AsuC2I CCSGG 5 cut(s) 374, 536, 698, 860, 1022
AvaI CYCGRG 6 cut(s) 1231, 1450, 1696, 1969, 2071, 2127
AvaII GGWCC 2 cut(s) 2052, 2315
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BccI CCATC 2 cut(s) 1070, 1816
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 2 cut(s) 1362, 1922
BcnI CCSGG 5 cut(s) 374, 536, 698, 860, 1022
BcoDI GTCTC 6 cut(s) 115, 279, 603, 765, 927, 1064
BfaI CTAG 1 cut(s) 1493
BfmI CTRYAG 4 cut(s) 156, 1149, 1161, 1531
BfoI RGCGCY 3 cut(s) 341, 503, 665
Bme1390I CCNGG 7 cut(s) 374, 536, 698, 860, 1022, 1362, 1922
Bme18I GGWCC 2 cut(s) 2052, 2315
BmeT110I CYCGRG 6 cut(s) 1231, 1450, 1696, 1969, 2071, 2127
BmgT120I GGNCC 3 cut(s) 1154, 2052, 2315
BmiI GGNNCC 3 cut(s) 1662, 1947, 2054
BmrFI CCNGG 7 cut(s) 374, 536, 698, 860, 1022, 1362, 1922
BmrI ACTGGG 4 cut(s) 1187, 1607, 1733, 2006
BmuI ACTGGG 4 cut(s) 1187, 1607, 1733, 2006
BpmI CTGGAG 2 cut(s) 1797, 1964
Bpu10I CCTNAGC 3 cut(s) 1671, 1794, 2190
BpuEI CTTGAG 1 cut(s) 1193
BpuMI CCSGG 5 cut(s) 374, 536, 698, 860, 1022
Bsa29I ATCGAT 2 cut(s) 1214, 2115
BsaJI CCNNGG 2 cut(s) 1360, 2267
Bsc4I CCNNNNNNNGG 9 cut(s) 1066, 1174, 1264, 1366, 1474, 1594, 1720, 1993, 2063
Bse118I RCCGGY 5 cut(s) 22, 184, 346, 508, 670
Bse1I ACTGG 9 cut(s) 1193, 1283, 1376, 1439, 1613, 1662, 1739, 1799, 2012
Bse3DI GCAATG 1 cut(s) 1243
BseBI CCWGG 2 cut(s) 1362, 1922
BseCI ATCGAT 2 cut(s) 1214, 2115
BseDI CCNNGG 2 cut(s) 1360, 2267
BseGI GGATG 1 cut(s) 2175
BseLI CCNNNNNNNGG 9 cut(s) 1066, 1174, 1264, 1366, 1474, 1594, 1720, 1993, 2063
BseMI GCAATG 1 cut(s) 1243
BseMII CTCAG 4 cut(s) 1416, 1662, 1785, 2181
BseNI ACTGG 9 cut(s) 1193, 1283, 1376, 1439, 1613, 1662, 1739, 1799, 2012
BseX3I CGGCCG 1 cut(s) 24
BseYI CCCAGC 5 cut(s) 334, 496, 658, 820, 982
Bsh1236I CGCG 1 cut(s) 1994
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 26, 1155
BshVI ATCGAT 2 cut(s) 1214, 2115
BsiEI CGRYCG 1 cut(s) 27
BsiHKCI CYCGRG 6 cut(s) 1231, 1450, 1696, 1969, 2071, 2127
BslI CCNNNNNNNGG 9 cut(s) 1066, 1174, 1264, 1366, 1474, 1594, 1720, 1993, 2063
BsmAI GTCTC 6 cut(s) 115, 279, 603, 765, 927, 1064
BsnI GGCC 2 cut(s) 26, 1155
BsoBI CYCGRG 6 cut(s) 1231, 1450, 1696, 1969, 2071, 2127
BspANI GGCC 2 cut(s) 26, 1155
BspCNI CTCAG 4 cut(s) 1417, 1663, 1786, 2182
BspDI ATCGAT 2 cut(s) 1214, 2115
BspFNI CGCG 1 cut(s) 1994
BspLI GGNNCC 3 cut(s) 1662, 1947, 2054
BspMAI CTGCAG 1 cut(s) 1165
BspQI GCTCTTC 4 cut(s) 1322, 1337, 1565, 1838
BsrBI CCGCTC 2 cut(s) 46, 208
BsrDI GCAATG 1 cut(s) 1243
BsrFI RCCGGY 5 cut(s) 22, 184, 346, 508, 670
BsrI ACTGG 9 cut(s) 1193, 1283, 1376, 1439, 1613, 1662, 1739, 1799, 2012
BssAI RCCGGY 5 cut(s) 22, 184, 346, 508, 670
BssECI CCNNGG 2 cut(s) 1360, 2267
BssT1I CCWWGG 1 cut(s) 2267
Bst2UI CCWGG 2 cut(s) 1362, 1922
BstC8I GCNNGC 8 cut(s) 24, 33, 186, 195, 348, 510, 1650, 1773
BstDEI CTNAG 4 cut(s) 1425, 1671, 1794, 2190
BstF5I GGATG 1 cut(s) 2175
BstFNI CGCG 1 cut(s) 1994
BstH2I RGCGCY 3 cut(s) 341, 503, 665
BstMAI GTCTC 6 cut(s) 115, 279, 603, 765, 927, 1064
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 9 cut(s) 28, 32, 190, 194, 352, 514, 1329, 1394, 1789
BstNI CCWGG 2 cut(s) 1362, 1922
BstNSI RCATGY 1 cut(s) 1775
BstSCI CCNGG 7 cut(s) 372, 534, 696, 858, 1020, 1360, 1920
BstSFI CTRYAG 4 cut(s) 156, 1149, 1161, 1531
BstUI CGCG 1 cut(s) 1994
BstZI CGGCCG 1 cut(s) 24
Bsu15I ATCGAT 2 cut(s) 1214, 2115
BsuRI GGCC 2 cut(s) 26, 1155
BsuTUI ATCGAT 2 cut(s) 1214, 2115
BtsCI GGATG 1 cut(s) 2175
BtsIMutI CAGTG 1 cut(s) 1958
Cac8I GCNNGC 8 cut(s) 24, 33, 186, 195, 348, 510, 1650, 1773
CaiI CAGNNNCTG 4 cut(s) 1259, 1382, 1469, 1942
Cfr10I RCCGGY 5 cut(s) 22, 184, 346, 508, 670
Cfr13I GGNCC 3 cut(s) 1154, 2052, 2315
ClaI ATCGAT 2 cut(s) 1214, 2115
CsiI ACCWGGT 1 cut(s) 1920
CviAII CATG 5 cut(s) 40, 1622, 1715, 1772, 2207
DdeI CTNAG 4 cut(s) 1425, 1671, 1794, 2190
DraIII CACNNNGTG 1 cut(s) 2063
EaeI YGGCCR 1 cut(s) 24
EagI CGGCCG 1 cut(s) 24
EciI GGCGGA 1 cut(s) 1740
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 2267
Eco47I GGWCC 2 cut(s) 2052, 2315
Eco52I CGGCCG 1 cut(s) 24
Eco88I CYCGRG 6 cut(s) 1231, 1450, 1696, 1969, 2071, 2127
EcoRII CCWGG 2 cut(s) 1360, 1920
EcoT14I CCWWGG 1 cut(s) 2267
ErhI CCWWGG 1 cut(s) 2267
FaeI CATG 5 cut(s) 43, 1625, 1718, 1775, 2210
FatI CATG 5 cut(s) 39, 1621, 1714, 1771, 2206
FauI CCCGC 2 cut(s) 1176, 1645
FblI GTMKAC 2 cut(s) 1917, 2275
FokI GGATG 1 cut(s) 2162
FspBI CTAG 1 cut(s) 1493
FspI TGCGCA 1 cut(s) 1208
GsaI CCCAGC 5 cut(s) 338, 500, 662, 824, 986
GsuI CTGGAG 2 cut(s) 1797, 1964
HaeII RGCGCY 3 cut(s) 341, 503, 665
HaeIII GGCC 2 cut(s) 26, 1155
Hin1II CATG 5 cut(s) 43, 1625, 1718, 1775, 2210
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
Hpy166II GTNNAC 3 cut(s) 1111, 1918, 2276
Hpy188I TCNGA 7 cut(s) 225, 1419, 1548, 1665, 1788, 1938, 2160
Hpy8I GTNNAC 3 cut(s) 1111, 1918, 2276
HpyAV CCTTC 2 cut(s) 1237, 1757
HpyCH4V TGCA 2 cut(s) 1163, 1625
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 32, 190, 194, 352, 514, 1329, 1394, 1789
HpyF3I CTNAG 4 cut(s) 1425, 1671, 1794, 2190
Hsp92II CATG 5 cut(s) 43, 1625, 1718, 1775, 2210
KroI GCCGGC 4 cut(s) 22, 184, 346, 508
KroNI GCCGGC 4 cut(s) 24, 186, 348, 510
LguI GCTCTTC 4 cut(s) 1322, 1337, 1565, 1838
MabI ACCWGGT 1 cut(s) 1920
MaeI CTAG 1 cut(s) 1493
MaeIII GTNAC 3 cut(s) 1128, 1441, 2253
MbiI CCGCTC 2 cut(s) 46, 208
MfeI CAATTG 6 cut(s) 297, 459, 621, 783, 945, 1086
MlyI GAGTC 2 cut(s) 1436, 2229
MmeI TCCRAC 1 cut(s) 2138
MroNI GCCGGC 4 cut(s) 22, 184, 346, 508
MseI TTAA 2 cut(s) 1290, 2037
MspR9I CCNGG 7 cut(s) 374, 536, 698, 860, 1022, 1362, 1922
MunI CAATTG 6 cut(s) 297, 459, 621, 783, 945, 1086
MvaI CCWGG 2 cut(s) 1362, 1922
MvnI CGCG 1 cut(s) 1994
MwoI GCNNNNNNNGC 9 cut(s) 28, 32, 190, 194, 352, 514, 1329, 1394, 1789
NaeI GCCGGC 4 cut(s) 24, 186, 348, 510
NciI CCSGG 5 cut(s) 374, 536, 698, 860, 1022
NgoMIV GCCGGC 4 cut(s) 22, 184, 346, 508
NlaIII CATG 5 cut(s) 43, 1625, 1718, 1775, 2210
NlaIV GGNNCC 3 cut(s) 1662, 1947, 2054
NmeAIII GCCGAG 2 cut(s) 52, 1845
NsbI TGCGCA 1 cut(s) 1208
NspI RCATGY 1 cut(s) 1775
PaeI GCATGC 1 cut(s) 1775
PaeR7I CTCGAG 2 cut(s) 2071, 2127
PciSI GCTCTTC 4 cut(s) 1322, 1337, 1565, 1838
PdiI GCCGGC 4 cut(s) 24, 186, 348, 510
PflMI CCANNNNNTGG 1 cut(s) 2063
PfoI TCCNGGA 5 cut(s) 372, 534, 696, 858, 1020
PleI GAGTC 2 cut(s) 1435, 2228
PpsI GAGTC 2 cut(s) 1435, 2228
Psp6I CCWGG 2 cut(s) 1360, 1920
PspFI CCCAGC 5 cut(s) 334, 496, 658, 820, 982
PspGI CCWGG 2 cut(s) 1360, 1920
PspN4I GGNNCC 3 cut(s) 1662, 1947, 2054
PspPI GGNCC 3 cut(s) 1154, 2052, 2315
PspXI VCTCGAGB 1 cut(s) 2127
PsrI GAACNNNNNNTAC 2 cut(s) 1113, 1145
PstI CTGCAG 1 cut(s) 1165
PstNI CAGNNNCTG 4 cut(s) 1259, 1382, 1469, 1942
SapI GCTCTTC 4 cut(s) 1322, 1337, 1565, 1838
SaqAI TTAA 2 cut(s) 1290, 2037
Sau96I GGNCC 3 cut(s) 1154, 2052, 2315
SchI GAGTC 2 cut(s) 1436, 2229
ScrFI CCNGG 7 cut(s) 374, 536, 698, 860, 1022, 1362, 1922
SexAI ACCWGGT 1 cut(s) 1920
SfcI CTRYAG 4 cut(s) 156, 1149, 1161, 1531
Sfr274I CTCGAG 2 cut(s) 2071, 2127
SinI GGWCC 2 cut(s) 2052, 2315
SlaI CTCGAG 2 cut(s) 2071, 2127
SmlI CTYRAG 3 cut(s) 1172, 2071, 2127
SmoI CTYRAG 3 cut(s) 1172, 2071, 2127
SphI GCATGC 1 cut(s) 1775
SspMI CTAG 1 cut(s) 1493
StyD4I CCNGG 7 cut(s) 372, 534, 696, 858, 1020, 1360, 1920
StyI CCWWGG 1 cut(s) 2267
TaqII GACCGA 1 cut(s) 2303
TauI GCSGC 7 cut(s) 19, 38, 46, 181, 208, 505, 667
Tru1I TTAA 2 cut(s) 1290, 2037
Tru9I TTAA 2 cut(s) 1290, 2037
TscAI CASTG 1 cut(s) 1965
TspGWI ACGGA 8 cut(s) 141, 303, 465, 627, 789, 951, 2055, 2293
TspRI CASTG 1 cut(s) 1965
Van91I CCANNNNNTGG 1 cut(s) 2063
VpaK11BI GGWCC 2 cut(s) 2052, 2315
XapI RAATTY 3 cut(s) 315, 1300, 2029
XceI RCATGY 1 cut(s) 1775
XcmI CCANNNNNNNNNTGG 2 cut(s) 2213, 2265
XhoI CTCGAG 2 cut(s) 2071, 2127
XmiI GTMKAC 2 cut(s) 1917, 2275
XspI CTAG 1 cut(s) 1493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.