Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004518

Physical Location & Seq

Genomic Coordinates Forward (+)

3695645 .. 3697799

Gene Structure 2155 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1046300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGATGTGATTGTGGATCCAGGGGAGGGGCAAATCGGTCAAAGAAAATCCGGGATGAACAAGTACACAATTGTCTGCGCTCTCTTGGCCTCCGCAAATTCCATTCTTTTGGGTTATGATATCGGAGTGATGAGTGGTGCGGTGCTCTACATCAAAGAGAATCTCAAGATCACATCAACCCAAGTGGAAATTTTGGTAGGAACCCTAAACGTGTTCTCTTTGGTAGGATCACTAGCATCCGGCAAGACTTCGGATTATATAGGCCGGCGTTACACTATAGTCCTAGCCGCTTGCACATTCCTAATAGGTGCACTCCTCATGGGCCTAGCGCCGTCCTACCCATTTCTAATGGCGGGACGTGTCGTGGCCGGCATTGGTGTCGGCTACGCCCTCATGATCGCTCCCGTCTACACAGCTGAGCTATCCCCCGCCATGAGCCGGGGATTCCTCACCTCTCTTCCGGAAGTGTTCATCACATTCGGGTTATTACTCGGTTATATTTGTAACTACGCCTTGTCCGGCCTACCGCCGCACATAAATTGGAGGCTGATGCTCGGGTTGGCGGCGGTGCCGGCAATCGGAATTGGGTTGGGAGTTATAGCAATGCCTGAGTCCCCTCGTTGGCTTGTGATGAAGGGGAAATTAAGGGAAGCAAAAGAGGTCTTACTGAAAACATCATGTTCTGAAGAGGAAGCTCAACTGAGGCTTGAGGAGATGAGTAAGGCTGCTTTGGAATTACCCACAGGCCCATTAACGGCCCAGGAAGATTCTTCGGCTGGTGGGCCTGGACGAGGGGCTTGGAAGGAGCTCTTGCTGCGTCCTTCGAAGCCCATTCGTCGGATGCTCGTTGCTGCCATAGGGATCAACTTTTTCATGCAAGCTTCCGGCAACGACGCCGTCGTTTACTACTGCCCGGAAGTATTTAGGGCAGCTGGAATTCACAGCAAGAAACAGCTCTTCGGTATTAATGTCATAATGGGCCTTGCCAAGACATCCTTTGTTTTCATCTCGGCGAGTTACTTGGACCGGTTTGGGAGGCGGCCCCTTTTGTTATTGGGCTCGGTCGGGATGGTGGTCTCGTTAGCTGGGCTGGGCCTGGGATCAAAATTTCTCGAGCATTCTAGTAGTAAGCCCACATGGGCCATTGCCTTGTGTATCGTGGCTGTTTGCGCCGATGTTTCGTTCTTTTCGATTGGGCTTGGGCCCATAACTTGGGTCTACTCATCGGAGATCTTTCCGATGAGGTTACGGGCCCAGGGTTCGAGTCTAGCCATATCGGTGAACCGGTTGGTAAGCGGGGTGGTGTCGATGACATTTCTAACCATCTCTAACAAGATAACATTCGGGGGAGTGTTTTTCGTGCTATCCGGAATAATGGCGGTTGCAACGGTGTTCTTTTACTTCTTTCTGCCAGAGACAAAAGGCAAGACCTTGGAAGAAATGGGGACGATTTTTGAAGACAAGGACACAATAAATGTGAATAGGGTGAGGGAAATGAGAGAGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005354 GO:0005355 GO:0005365 GO:0005402 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009653 GO:0009791 GO:0010015 GO:0010101 GO:0010102 GO:0010311 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015146 GO:0015148 GO:0015149 GO:0015166 GO:0015168 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015575 GO:0015576 GO:0015591 GO:0015672 GO:0015749 GO:0015750 GO:0015752 GO:0015753 GO:0015757 GO:0015791 GO:0015793 GO:0015795 GO:0015797 GO:0015798 GO:0015850 GO:0016020 GO:0022622 GO:0022804 GO:0022857 GO:0022890 GO:0032501 GO:0032502 GO:0034219 GO:0034220 GO:0044464 GO:0046323 GO:0048364 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071944 GO:0090696 GO:0090697 GO:0090698 GO:0098655 GO:0098660 GO:0098662 GO:0099402 GO:1901618 GO:1902600 GO:1904659 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

503

Amino Acids

53.99

Weight (kDa)

9.1

Isoelectric Point (pI)

36.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	27 - 485	6.1e-106	Sugar (and other) transporter
MFS_1	PF07690	34 - 434	1.1e-32	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004518)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04750 FvH4_1g04750 FvH4_1g04750 FvH4_1g04760 FvH4_1g04760
malus_domestica	MD02G1046300.v1.1 MD15G1184500.v1.1
prunus_persica	Prupe.7G231500_v2.0.a1 Prupe.7G231600_v2.0.a1
pyrus_communis	pycom02g03880 pycom15g16610
rosa_chinensis	RchiOBHm_Chr2g0090351 RchiOBHm_Chr2g0090361
rosa_laevigata	RLG00000016100 RLG00000016102
rosa_multiflora	Rmu_sc0004514.1_g000004 Rmu_sc0006235.1_g000008
rosa_roxburghii	Rroxscaffold_2G00151230 Rroxscaffold_2G00151250
rosa_rugosa	Rorug02G0006700 Rorug02G0006800 Rorug02G0006800
rosa_samantha	Rh2AG052700 Rh2AG052800 Rh2BG051400 Rh2BG051600 Rh2CG053600 Rh2CG053700 Rh2DG052700 Rh2DG052800
rosa_wichuraiana	Rw2G004410 Rw2G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	568
AccB7I	CCANNNNNTGG	1 cut(s)	1212
AccI	GTMKAC	2 cut(s)	408, 1218
AccIII	TCCGGA	2 cut(s)	460, 1367
AclWI	GGATC	5 cut(s)	11, 24, 235, 869, 1108
AcoI	YGGCCR	1 cut(s)	366
AcsI	RAATTY	4 cut(s)	97, 189, 936, 1106
AcuI	CTGAAG	1 cut(s)	705
AcyI	GRCGYC	1 cut(s)	894
AfaI	GTAC	1 cut(s)	65
AfiI	CCNNNNNNNGG	8 cut(s)	26, 438, 578, 621, 754, 791, 837, 1212
AflIII	ACRYGT	2 cut(s)	210, 358
AgeI	ACCGGT	2 cut(s)	1026, 1284
AgsI	TTSAA	1 cut(s)	1457
AjiI	CACGTC	1 cut(s)	359
AjnI	CCWGG	5 cut(s)	19, 759, 784, 1095, 1254
AluBI	AGCT	8 cut(s)	416, 421, 695, 808, 881, 932, 955, 1085
AluI	AGCT	8 cut(s)	416, 421, 695, 808, 881, 932, 955, 1085
Alw21I	GWGCWC	3 cut(s)	147, 313, 810
Alw26I	GTCTC	2 cut(s)	1081, 1409
Alw44I	GTGCAC	1 cut(s)	309
AlwI	GGATC	5 cut(s)	11, 24, 235, 869, 1108
Ama87I	CYCGRG	2 cut(s)	554, 1112
Aor13HI	TCCGGA	2 cut(s)	460, 1367
ApaI	GGGCCC	2 cut(s)	1206, 1255
ApaLI	GTGCAC	1 cut(s)	309
ApeKI	GCWGC	4 cut(s)	725, 814, 851, 929
ApoI	RAATTY	4 cut(s)	97, 189, 936, 1106
AseI	ATTAAT	1 cut(s)	966
AsiGI	ACCGGT	2 cut(s)	1026, 1284
AspLEI	GCGC	3 cut(s)	80, 331, 1172
AsuC2I	CCSGG	3 cut(s)	52, 440, 914
AsuHPI	GGTGA	3 cut(s)	442, 1291, 1498
AsuII	TTCGAA	1 cut(s)	824
AvaI	CYCGRG	2 cut(s)	554, 1112
AvaII	GGWCC	1 cut(s)	1024
BaeGI	GKGCMC	3 cut(s)	313, 1206, 1255
BamHI	GGATCC	1 cut(s)	16
BanI	GGYRCC	1 cut(s)	568
BanII	GRGCYC	4 cut(s)	810, 1061, 1206, 1255
BbsI	GAAGAC	1 cut(s)	1464
Bbv12I	GWGCWC	3 cut(s)	147, 313, 810
BbvI	GCAGC	4 cut(s)	712, 801, 838, 941
BccI	CCATC	2 cut(s)	1063, 1331
BceAI	ACGGC	3 cut(s)	316, 771, 881
BcgI	CGANNNNNNTGC	2 cut(s)	361, 395
BciT130I	CCWGG	5 cut(s)	21, 761, 786, 1097, 1256
BcnI	CCSGG	3 cut(s)	52, 440, 914
BcoDI	GTCTC	2 cut(s)	1081, 1409
BfaI	CTAG	5 cut(s)	233, 284, 326, 1122, 1268
BfmI	CTRYAG	1 cut(s)	276
BfoI	RGCGCY	1 cut(s)	332
BglII	AGATCT	1 cut(s)	1230
BisI	GCNGC	8 cut(s)	288, 530, 564, 726, 815, 852, 930, 1040
BlpI	GCTNAGC	1 cut(s)	417
BlsI	GCNGC	8 cut(s)	289, 531, 565, 727, 816, 853, 931, 1041
Bme1390I	CCNGG	8 cut(s)	21, 52, 440, 761, 786, 914, 1097, 1256
Bme18I	GGWCC	1 cut(s)	1024
BmeT110I	CYCGRG	2 cut(s)	554, 1112
BmgBI	CACGTC	1 cut(s)	359
BmiI	GGNNCC	6 cut(s)	18, 202, 570, 1043, 1204, 1253
BmrFI	CCNGG	8 cut(s)	21, 52, 440, 761, 786, 914, 1097, 1256
BmsI	GCATC	3 cut(s)	245, 540, 831
BpiI	GAAGAC	1 cut(s)	1464
Bpu1102I	GCTNAGC	1 cut(s)	417
Bpu14I	TTCGAA	1 cut(s)	824
BpuEI	CTTGAG	2 cut(s)	149, 728
BpuMI	CCSGG	3 cut(s)	52, 440, 914
BsaHI	GRCGYC	1 cut(s)	894
BsaI	GGTCTC	1 cut(s)	1081
BsaJI	CCNNGG	7 cut(s)	20, 439, 759, 1096, 1254, 1255, 1431
BsaWI	WCCGGW	4 cut(s)	460, 1026, 1284, 1367
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 438, 578, 621, 754, 791, 837, 1212
Bse118I	RCCGGY	5 cut(s)	264, 368, 571, 1026, 1284
Bse3DI	GCAATG	2 cut(s)	609, 1143
BseAI	TCCGGA	2 cut(s)	460, 1367
BseBI	CCWGG	5 cut(s)	21, 761, 786, 1097, 1256
BseDI	CCNNGG	7 cut(s)	20, 439, 759, 1096, 1254, 1255, 1431
BseGI	GGATG	6 cut(s)	10, 60, 236, 846, 992, 1074
BseLI	CCNNNNNNNGG	8 cut(s)	26, 438, 578, 621, 754, 791, 837, 1212
BseMI	GCAATG	2 cut(s)	609, 1143
BseMII	CTCAG	3 cut(s)	408, 600, 692
BseRI	GAGGAG	2 cut(s)	305, 725
BseSI	GKGCMC	3 cut(s)	313, 1206, 1255
BseXI	GCAGC	4 cut(s)	712, 801, 838, 941
BseYI	CCCAGC	2 cut(s)	1085, 1090
Bsh1285I	CGRYCG	1 cut(s)	1065
BshNI	GGYRCC	1 cut(s)	568
BshTI	ACCGGT	2 cut(s)	1026, 1284
BsiEI	CGRYCG	1 cut(s)	1065
BsiHKAI	GWGCWC	3 cut(s)	147, 313, 810
BsiHKCI	CYCGRG	2 cut(s)	554, 1112
BslFI	GGGAC	3 cut(s)	369, 598, 1459
BslI	CCNNNNNNNGG	8 cut(s)	26, 438, 578, 621, 754, 791, 837, 1212
BsmAI	GTCTC	2 cut(s)	1081, 1409
BsmFI	GGGAC	3 cut(s)	369, 598, 1459
BsmI	GAATGC	1 cut(s)	1117
Bso31I	GGTCTC	1 cut(s)	1081
BsoBI	CYCGRG	2 cut(s)	554, 1112
Bsp119I	TTCGAA	1 cut(s)	824
Bsp120I	GGGCCC	2 cut(s)	1202, 1251
Bsp1286I	GDGCHC	6 cut(s)	147, 313, 810, 1061, 1206, 1255
Bsp13I	TCCGGA	2 cut(s)	460, 1367
Bsp143I	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
Bsp1720I	GCTNAGC	1 cut(s)	417
BspCNI	CTCAG	3 cut(s)	409, 601, 693
BspEI	TCCGGA	2 cut(s)	460, 1367
BspHI	TCATGA	1 cut(s)	393
BspLI	GGNNCC	6 cut(s)	18, 202, 570, 1043, 1204, 1253
BspPI	GGATC	5 cut(s)	11, 24, 235, 869, 1108
BspQI	GCTCTTC	1 cut(s)	962
BspT104I	TTCGAA	1 cut(s)	824
BspT107I	GGYRCC	1 cut(s)	568
BspTNI	GGTCTC	1 cut(s)	1081
BsrDI	GCAATG	2 cut(s)	609, 1143
BsrFI	RCCGGY	5 cut(s)	264, 368, 571, 1026, 1284
BssAI	RCCGGY	5 cut(s)	264, 368, 571, 1026, 1284
BssECI	CCNNGG	7 cut(s)	20, 439, 759, 1096, 1254, 1255, 1431
BssMI	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
BssNI	GRCGYC	1 cut(s)	894
BssT1I	CCWWGG	1 cut(s)	1431
Bst2UI	CCWGG	5 cut(s)	21, 761, 786, 1097, 1256
Bst4CI	ACNGT	1 cut(s)	1390
Bst6I	CTCTTC	3 cut(s)	462, 681, 962
BstACI	GRCGYC	1 cut(s)	894
BstBI	TTCGAA	1 cut(s)	824
BstC8I	GCNNGC	5 cut(s)	266, 292, 370, 573, 879
BstDEI	CTNAG	3 cut(s)	417, 609, 701
BstENI	CCTNNNNNAGG	1 cut(s)	789
BstF5I	GGATG	6 cut(s)	10, 60, 236, 846, 992, 1074
BstH2I	RGCGCY	1 cut(s)	332
BstHHI	GCGC	3 cut(s)	80, 331, 1172
BstKTI	GATC	7 cut(s)	19, 171, 230, 399, 864, 1103, 1233
BstMAI	GTCTC	2 cut(s)	1081, 1409
BstMBI	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
BstMCI	CGRYCG	1 cut(s)	1065
BstMWI	GCNNNNNNNGC	4 cut(s)	86, 572, 814, 1169
BstNI	CCWGG	5 cut(s)	21, 761, 786, 1097, 1256
BstSCI	CCNGG	8 cut(s)	19, 50, 438, 759, 784, 912, 1095, 1254
BstSFI	CTRYAG	1 cut(s)	276
BstSLI	GKGCMC	3 cut(s)	313, 1206, 1255
BstV1I	GCAGC	4 cut(s)	712, 801, 838, 941
BstV2I	GAAGAC	1 cut(s)	1464
BstX2I	RGATCY	2 cut(s)	16, 1230
BstXI	CCANNNNNNTGG	1 cut(s)	109
BstYI	RGATCY	2 cut(s)	16, 1230
BtrI	CACGTC	1 cut(s)	359
BtsCI	GGATG	6 cut(s)	10, 60, 236, 846, 992, 1074
Cac8I	GCNNGC	5 cut(s)	266, 292, 370, 573, 879
CciI	TCATGA	1 cut(s)	393
CfoI	GCGC	3 cut(s)	80, 331, 1172
Cfr10I	RCCGGY	5 cut(s)	264, 368, 571, 1026, 1284
CseI	GACGC	2 cut(s)	806, 902
Csp6I	GTAC	1 cut(s)	64
CspAI	ACCGGT	2 cut(s)	1026, 1284
CspCI	CAANNNNNGTGG	2 cut(s)	165, 200
CviAII	CATG	6 cut(s)	319, 394, 433, 678, 874, 1137
CviQI	GTAC	1 cut(s)	64
DdeI	CTNAG	3 cut(s)	417, 609, 701
DpnI	GATC	7 cut(s)	18, 170, 229, 398, 863, 1102, 1232
DpnII	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
EaeI	YGGCCR	1 cut(s)	366
Eam1104I	CTCTTC	3 cut(s)	462, 681, 962
EarI	CTCTTC	3 cut(s)	462, 681, 962
Ecl136II	GAGCTC	1 cut(s)	808
Eco130I	CCWWGG	1 cut(s)	1431
Eco24I	GRGCYC	4 cut(s)	810, 1061, 1206, 1255
Eco31I	GGTCTC	1 cut(s)	1081
Eco32I	GATATC	1 cut(s)	121
Eco47I	GGWCC	1 cut(s)	1024
Eco53kI	GAGCTC	1 cut(s)	808
Eco57I	CTGAAG	1 cut(s)	705
Eco88I	CYCGRG	2 cut(s)	554, 1112
EcoICRI	GAGCTC	1 cut(s)	808
EcoNI	CCTNNNNNAGG	1 cut(s)	789
EcoRI	GAATTC	1 cut(s)	936
EcoRII	CCWGG	5 cut(s)	19, 759, 784, 1095, 1254
EcoRV	GATATC	1 cut(s)	121
EcoT14I	CCWWGG	1 cut(s)	1431
EcoT38I	GRGCYC	4 cut(s)	810, 1061, 1206, 1255
ErhI	CCWWGG	1 cut(s)	1431
FaeI	CATG	6 cut(s)	322, 397, 436, 681, 877, 1140
FalI	AAGNNNNNCTT	4 cut(s)	794, 826, 980, 1012
FaqI	GGGAC	3 cut(s)	369, 598, 1459
FatI	CATG	6 cut(s)	318, 393, 432, 677, 873, 1136
FauI	CCCGC	3 cut(s)	346, 436, 1289
FblI	GTMKAC	2 cut(s)	408, 1218
Fnu4HI	GCNGC	8 cut(s)	288, 530, 564, 726, 815, 852, 930, 1040
FokI	GGATG	6 cut(s)	17, 67, 223, 853, 979, 1081
FriOI	GRGCYC	4 cut(s)	810, 1061, 1206, 1255
Fsp4HI	GCNGC	8 cut(s)	288, 530, 564, 726, 815, 852, 930, 1040
FspBI	CTAG	5 cut(s)	233, 284, 326, 1122, 1268
GlaI	GCGC	3 cut(s)	79, 330, 1171
GluI	GCNGC	8 cut(s)	288, 530, 564, 726, 815, 852, 930, 1040
GsaI	CCCAGC	2 cut(s)	1089, 1094
HaeII	RGCGCY	1 cut(s)	332
HgaI	GACGC	2 cut(s)	806, 902
HhaI	GCGC	3 cut(s)	80, 331, 1172
Hin1I	GRCGYC	1 cut(s)	894
Hin1II	CATG	6 cut(s)	322, 397, 436, 681, 877, 1140
Hin6I	GCGC	3 cut(s)	78, 329, 1170
HinP1I	GCGC	3 cut(s)	78, 329, 1170
HindIII	AAGCTT	1 cut(s)	879
HinfI	GANTC	5 cut(s)	160, 444, 611, 767, 1264
HphI	GGTGA	3 cut(s)	442, 1291, 1498
Hpy166II	GTNNAC	6 cut(s)	66, 311, 409, 904, 1219, 1282
Hpy188I	TCNGA	7 cut(s)	125, 253, 581, 685, 840, 1228, 1239
Hpy188III	TCNNGA	6 cut(s)	166, 394, 461, 1066, 1112, 1368
Hpy8I	GTNNAC	6 cut(s)	66, 311, 409, 904, 1219, 1282
Hpy99I	CGWCG	3 cut(s)	840, 896, 902
HpyAV	CCTTC	3 cut(s)	628, 796, 831
HpyCH4III	ACNGT	1 cut(s)	1390
HpyCH4IV	ACGT	2 cut(s)	210, 358
HpyCH4V	TGCA	4 cut(s)	294, 311, 877, 1385
HpyF10VI	GCNNNNNNNGC	4 cut(s)	86, 572, 814, 1169
HpyF3I	CTNAG	3 cut(s)	417, 609, 701
HpySE526I	ACGT	2 cut(s)	210, 358
Hsp92I	GRCGYC	1 cut(s)	894
Hsp92II	CATG	6 cut(s)	322, 397, 436, 681, 877, 1140
HspAI	GCGC	3 cut(s)	78, 329, 1170
Kpn2I	TCCGGA	2 cut(s)	460, 1367
KroI	GCCGGC	3 cut(s)	264, 368, 571
KroNI	GCCGGC	3 cut(s)	266, 370, 573
Kzo9I	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
LguI	GCTCTTC	1 cut(s)	962
LmnI	GCTCC	2 cut(s)	406, 805
Lsp1109I	GCAGC	4 cut(s)	712, 801, 838, 941
LweI	GCATC	3 cut(s)	245, 540, 831
MaeI	CTAG	5 cut(s)	233, 284, 326, 1122, 1268
MaeII	ACGT	2 cut(s)	210, 358
MaeIII	GTNAC	4 cut(s)	269, 503, 1016, 1245
MalI	GATC	7 cut(s)	18, 170, 229, 398, 863, 1102, 1232
MboI	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
MboII	GAAGA	7 cut(s)	449, 698, 762, 776, 949, 1448, 1469
MfeI	CAATTG	1 cut(s)	69
MflI	RGATCY	2 cut(s)	16, 1230
MhlI	GDGCHC	6 cut(s)	147, 313, 810, 1061, 1206, 1255
MluCI	AATT	9 cut(s)	69, 97, 189, 538, 582, 641, 734, 936, 1106
MlyI	GAGTC	2 cut(s)	620, 1273
MmeI	TCCRAC	1 cut(s)	818
MroI	TCCGGA	2 cut(s)	460, 1367
MroNI	GCCGGC	3 cut(s)	264, 368, 571
MseI	TTAA	3 cut(s)	644, 752, 966
MslI	CAYNNNNRTG	1 cut(s)	1277
MspA1I	CMGCKG	2 cut(s)	416, 932
MspR9I	CCNGG	8 cut(s)	21, 52, 440, 761, 786, 914, 1097, 1256
MunI	CAATTG	1 cut(s)	69
Mva1269I	GAATGC	1 cut(s)	1117
MvaI	CCWGG	5 cut(s)	21, 761, 786, 1097, 1256
MwoI	GCNNNNNNNGC	4 cut(s)	86, 572, 814, 1169
NaeI	GCCGGC	3 cut(s)	266, 370, 573
NciI	CCSGG	3 cut(s)	52, 440, 914
NdeII	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
NgoMIV	GCCGGC	3 cut(s)	264, 368, 571
NlaIII	CATG	6 cut(s)	322, 397, 436, 681, 877, 1140
NlaIV	GGNNCC	6 cut(s)	18, 202, 570, 1043, 1204, 1253
NmeAIII	GCCGAG	1 cut(s)	989
NspV	TTCGAA	1 cut(s)	824
PaeR7I	CTCGAG	1 cut(s)	1112
PagI	TCATGA	1 cut(s)	393
PasI	CCCWGGG	1 cut(s)	1255
PciSI	GCTCTTC	1 cut(s)	962
PcsI	WCGNNNNNNNCGW	2 cut(s)	897, 1187
PctI	GAATGC	1 cut(s)	1117
PdiI	GCCGGC	3 cut(s)	266, 370, 573
PfeI	GAWTC	3 cut(s)	160, 444, 767
PflFI	GACNNNGTC	1 cut(s)	896
PflMI	CCANNNNNTGG	1 cut(s)	1212
PfoI	TCCNGGA	1 cut(s)	50
PinAI	ACCGGT	2 cut(s)	1026, 1284
PkrI	GCNGC	8 cut(s)	289, 531, 565, 727, 816, 853, 931, 1041
PleI	GAGTC	2 cut(s)	619, 1272
PpsI	GAGTC	2 cut(s)	619, 1272
PshBI	ATTAAT	1 cut(s)	966
Psp124BI	GAGCTC	1 cut(s)	810
Psp6I	CCWGG	5 cut(s)	19, 759, 784, 1095, 1254
PspFI	CCCAGC	2 cut(s)	1085, 1090
PspGI	CCWGG	5 cut(s)	19, 759, 784, 1095, 1254
PspN4I	GGNNCC	6 cut(s)	18, 202, 570, 1043, 1204, 1253
PspOMI	GGGCCC	2 cut(s)	1202, 1251
PsuI	RGATCY	2 cut(s)	16, 1230
PsyI	GACNNNGTC	1 cut(s)	896
PvuII	CAGCTG	2 cut(s)	416, 932
RsaI	GTAC	1 cut(s)	65
RsaNI	GTAC	1 cut(s)	64
RseI	CAYNNNNRTG	1 cut(s)	1277
SacI	GAGCTC	1 cut(s)	810
SapI	GCTCTTC	1 cut(s)	962
SaqAI	TTAA	3 cut(s)	644, 752, 966
SatI	GCNGC	8 cut(s)	288, 530, 564, 726, 815, 852, 930, 1040
Sau3AI	GATC	7 cut(s)	16, 168, 227, 396, 861, 1100, 1230
SchI	GAGTC	2 cut(s)	620, 1273
ScrFI	CCNGG	8 cut(s)	21, 52, 440, 761, 786, 914, 1097, 1256
SduI	GDGCHC	6 cut(s)	147, 313, 810, 1061, 1206, 1255
SfaNI	GCATC	3 cut(s)	245, 540, 831
SfcI	CTRYAG	1 cut(s)	276
Sfr274I	CTCGAG	1 cut(s)	1112
SfuI	TTCGAA	1 cut(s)	824
SinI	GGWCC	1 cut(s)	1024
SlaI	CTCGAG	1 cut(s)	1112
SmiMI	CAYNNNNRTG	1 cut(s)	1277
SmlI	CTYRAG	3 cut(s)	164, 707, 1112
SmoI	CTYRAG	3 cut(s)	164, 707, 1112
Sse9I	AATT	9 cut(s)	69, 97, 189, 538, 582, 641, 734, 936, 1106
SspMI	CTAG	5 cut(s)	233, 284, 326, 1122, 1268
SstI	GAGCTC	1 cut(s)	810
StyD4I	CCNGG	8 cut(s)	19, 50, 438, 759, 784, 912, 1095, 1254
StyI	CCWWGG	1 cut(s)	1431
TaaI	ACNGT	1 cut(s)	1390
TaiI	ACGT	2 cut(s)	213, 361
TaqI	TCGA	5 cut(s)	824, 1113, 1190, 1262, 1307
TaqII	GACCGA	2 cut(s)	26, 1051
TasI	AATT	9 cut(s)	69, 97, 189, 538, 582, 641, 734, 936, 1106
TatI	WGTACW	1 cut(s)	63
TauI	GCSGC	4 cut(s)	290, 532, 566, 1042
TfiI	GAWTC	3 cut(s)	160, 444, 767
Tru1I	TTAA	3 cut(s)	644, 752, 966
Tru9I	TTAA	3 cut(s)	644, 752, 966
TseI	GCWGC	4 cut(s)	725, 814, 851, 929
TspDTI	ATGAA	5 cut(s)	71, 460, 647, 862, 994
Tth111I	GACNNNGTC	1 cut(s)	896
Van91I	CCANNNNNTGG	1 cut(s)	1212
VneI	GTGCAC	1 cut(s)	309
VpaK11BI	GGWCC	1 cut(s)	1024
VspI	ATTAAT	1 cut(s)	966
XagI	CCTNNNNNAGG	1 cut(s)	789
XapI	RAATTY	4 cut(s)	97, 189, 936, 1106
XhoI	CTCGAG	1 cut(s)	1112
XmiI	GTMKAC	2 cut(s)	408, 1218
XspI	CTAG	5 cut(s)	233, 284, 326, 1122, 1268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.