Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004518

Physical Location & Seq

Genomic Coordinates Forward (+)

14555622 .. 14557864

Gene Structure 2243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1184500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGAGCCAAGTAAGAAGGACCACCTCCATCCTCTGCTTGCTGATGATTCAGAATCTTCATCGCAGGCAGATCATCGATGTGAACCCGGAAAGCCGAAAGCACCGGAAGTTCATACGCAGCCCGGTCTCAACAAATATGCTCTTGCCGGTGCTATATTGGCTTCCACAAACTCTGTTCTATTGGGCTATGATATTGGGGTGATGAGTGGAGCTTCTCTTTTTATCAGAGAGGACTTCAATCTTTCATCAACGCAAGTAGAAATCTTGGTTGGTTCTCTAAATGTATGTTCATTGATCGGATCACTTGCCTCCGGCAAGACGTCGGATATTATCGGCAGGCGTTACACTATAGTCCTGGCTGCGTTAACATTTTTAATAGGTGCACTTCTAATGGGACTAGCACCATCCTTCCCATTTCTCTTAGCCGGTAGACTTGTGGCCGGAATCGGAGTAGGCTACTCCCTCATGATTGCCCCAGTTTACGTAGCCGAGCTCTCGCCAGCCCTCACCCGAGGGTTTCTCACCTCTCTCCCGGAGGTGTTCATCAACGGCGGAATACTGCTCGGGTATATCTCCAACTATGCTTTATCAGGCATCCCACAAAACATGAATTGGAGACTTATGCTTGGACTTGCAGCTTTGCCTGCTATTGCTGTTGCATTGGGTGTGCTAGCAATGCCTGAGTCCCCTAGATGGTTGGTCATGAAAGGCCGGCTTGACGAAGCGACGCAGGTTTTGATAAGAACATCTGAAACCAAAGAGGAAGCTGAATTGAGGCTCAAAGAGATAGCAAAAGCTGCCTCGTTGTTGCATGCTAGTACCTCTTCAAACAACTGGCATGGACAAGGGGTGTGGAGAGAGTTACTAGTGAGGCCCACGAAGCCGATCCGCCGCATTCTAATAGCTGCCATCGGAGTCAACTTCTTCATGCAAGCCTCAGGCAATGACGCGGTCGTGTACTATAGCCCAGAGGTATTCCGAGACGCCGGAATTCACAACAAGAGGCATCTTGTGGGTGTCACAATCCTCATGGGCATTGCCAAGACTTTCTTTGTTTTGGTTTCGGCACTCTTCCTGGACCGGTACGGACGACGACCCCTTTTGCTGTTAGGCTCTTTCGGCATGGCGATCTCATTGGCGGCTTTGGGATTTGGGTCCAAGTTTCTTGAGTACTCGAGTGAGAGGCCTGAATGGGCTATTGCGCTTTGTGTGATGGCTGTTTGTTCTGCGGCTTCTTTCTTCTCGATTGGCCTTGGGCCCATCACATGGGTATATTCGTCGGAAATATTTCCCATGAGGTTGAGGGCCCAAGGCTCGAGCTTGGCCATCGGAGTGAACAGATTGGTGAGTGGGTTAGTGGCCATGACATTTCTGAGCATTTCTAGGGAGATTACATTCGGAGGGATGTTTTTTGCGCTTGCAGGAGTAATGTTGGTTGGCACTATTTTCTTTTACATCAATTTGCCAGAAACCAAAGGGAAGAGCTTAGAAGAAATTGGGGCTCTATTTGAGGACGAGAGGGACGAGCATCAAATGCTTCTTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005354 GO:0005355 GO:0005365 GO:0005402 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009653 GO:0009791 GO:0010015 GO:0010101 GO:0010102 GO:0010311 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015146 GO:0015148 GO:0015149 GO:0015166 GO:0015168 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015575 GO:0015576 GO:0015591 GO:0015672 GO:0015749 GO:0015750 GO:0015752 GO:0015753 GO:0015757 GO:0015791 GO:0015793 GO:0015795 GO:0015797 GO:0015798 GO:0015850 GO:0016020 GO:0022622 GO:0022804 GO:0022857 GO:0022890 GO:0032501 GO:0032502 GO:0034219 GO:0034220 GO:0044464 GO:0046323 GO:0048364 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071944 GO:0090696 GO:0090697 GO:0090698 GO:0098655 GO:0098660 GO:0098662 GO:0099402 GO:1901618 GO:1902600 GO:1904659 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

516

Amino Acids

55.57

Weight (kDa)

7.71

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	51 - 503	7.1e-110	Sugar (and other) transporter
MFS_1	PF07690	59 - 452	1.5e-32	Major Facilitator Superfamily
MFS_1	PF07690	342 - 496	2.2e-07	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004518)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04750 FvH4_1g04750 FvH4_1g04750 FvH4_1g04760 FvH4_1g04760
malus_domestica	MD02G1046300.v1.1 MD15G1184500.v1.1
prunus_persica	Prupe.7G231500_v2.0.a1 Prupe.7G231600_v2.0.a1
pyrus_communis	pycom02g03880 pycom15g16610
rosa_chinensis	RchiOBHm_Chr2g0090351 RchiOBHm_Chr2g0090361
rosa_laevigata	RLG00000016100 RLG00000016102
rosa_multiflora	Rmu_sc0004514.1_g000004 Rmu_sc0006235.1_g000008
rosa_roxburghii	Rroxscaffold_2G00151230 Rroxscaffold_2G00151250
rosa_rugosa	Rorug02G0006700 Rorug02G0006800 Rorug02G0006800
rosa_samantha	Rh2AG052700 Rh2AG052800 Rh2BG051400 Rh2BG051600 Rh2CG053600 Rh2CG053700 Rh2DG052700 Rh2DG052800
rosa_wichuraiana	Rw2G004410 Rw2G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	323
Acc36I	ACCTGC	1 cut(s)	721
AccB7I	CCANNNNNTGG	1 cut(s)	1266
AccI	GTMKAC	1 cut(s)	430
AccII	CGCG	1 cut(s)	950
AciI	CCGC	6 cut(s)	552, 889, 892, 950, 1139, 1229
AclWI	GGATC	2 cut(s)	307, 880
AcoI	YGGCCR	3 cut(s)	438, 1323, 1359
AcsI	RAATTY	1 cut(s)	990
AcyI	GRCGYC	2 cut(s)	320, 984
AfaI	GTAC	4 cut(s)	820, 959, 1085, 1172
AfiI	CCNNNNNNNGG	2 cut(s)	693, 1266
AgeI	ACCGGT	1 cut(s)	1080
AgsI	TTSAA	2 cut(s)	238, 828
AhlI	ACTAGT	1 cut(s)	865
AjnI	CCWGG	2 cut(s)	354, 1074
AjuI	GAANNNNNNNTTGG	2 cut(s)	252, 284
AluBI	AGCT	8 cut(s)	212, 493, 638, 767, 797, 905, 1320, 1485
AluI	AGCT	8 cut(s)	212, 493, 638, 767, 797, 905, 1320, 1485
Alw21I	GWGCWC	2 cut(s)	385, 495
Alw26I	GTCTC	3 cut(s)	131, 610, 975
Alw44I	GTGCAC	1 cut(s)	381
AlwI	GGATC	2 cut(s)	307, 880
Ama87I	CYCGRG	4 cut(s)	510, 563, 1174, 1315
AoxI	GGCC	9 cut(s)	438, 709, 872, 1184, 1249, 1256, 1305, 1323, 1359
ApaI	GGGCCC	2 cut(s)	1260, 1309
ApaLI	GTGCAC	1 cut(s)	381
ApeKI	GCWGC	5 cut(s)	118, 359, 635, 797, 905
ApoI	RAATTY	1 cut(s)	990
ArsI	GACNNNNNNTTYG	2 cut(s)	718, 750
AsiGI	ACCGGT	1 cut(s)	1080
Asp700I	GAANNNNTTC	1 cut(s)	1287
AspLEI	GCGC	2 cut(s)	1204, 1417
AspS9I	GGNCC	8 cut(s)	19, 873, 1078, 1155, 1256, 1257, 1305, 1306
AsuC2I	CCSGG	3 cut(s)	87, 123, 533
AsuHPI	GGTGA	4 cut(s)	211, 499, 514, 1357
AsuNHI	GCTAGC	1 cut(s)	670
AvaI	CYCGRG	4 cut(s)	510, 563, 1174, 1315
AvaII	GGWCC	3 cut(s)	19, 1078, 1155
AxyI	CCTNAGG	1 cut(s)	937
BaeGI	GKGCMC	3 cut(s)	385, 1260, 1309
BalI	TGGCCA	2 cut(s)	1325, 1361
BanII	GRGCYC	4 cut(s)	495, 1260, 1309, 1504
Bbv12I	GWGCWC	2 cut(s)	385, 495
BbvI	GCAGC	5 cut(s)	130, 346, 647, 784, 892
BccI	CCATC	7 cut(s)	36, 412, 687, 917, 1207, 1268, 1334
BceAI	ACGGC	1 cut(s)	565
BciT130I	CCWGG	2 cut(s)	356, 1076
BcnI	CCSGG	3 cut(s)	87, 123, 533
BcoDI	GTCTC	3 cut(s)	131, 610, 975
BcuI	ACTAGT	1 cut(s)	865
BfaI	CTAG	6 cut(s)	398, 671, 690, 816, 866, 1383
BfmI	CTRYAG	2 cut(s)	348, 961
BfuAI	ACCTGC	1 cut(s)	721
BisI	GCNGC	8 cut(s)	119, 360, 636, 798, 892, 906, 1140, 1230
BlsI	GCNGC	8 cut(s)	120, 361, 637, 799, 893, 907, 1141, 1231
BmcAI	AGTACT	1 cut(s)	1172
Bme1390I	CCNGG	5 cut(s)	87, 123, 356, 533, 1076
Bme18I	GGWCC	3 cut(s)	19, 1078, 1155
BmeT110I	CYCGRG	4 cut(s)	510, 563, 1174, 1315
BmgT120I	GGNCC	8 cut(s)	19, 873, 1078, 1155, 1256, 1257, 1305, 1306
BmiI	GGNNCC	4 cut(s)	6, 1156, 1258, 1307
BmrFI	CCNGG	5 cut(s)	87, 123, 356, 533, 1076
BmrI	ACTGGG	1 cut(s)	470
BmsI	GCATC	3 cut(s)	603, 1015, 1537
BmtI	GCTAGC	1 cut(s)	674
BmuI	ACTGGG	1 cut(s)	470
BplI	GAGNNNNNCTC	2 cut(s)	504, 536
BpuEI	CTTGAG	1 cut(s)	1187
BpuMI	CCSGG	3 cut(s)	87, 123, 533
Bsa29I	ATCGAT	1 cut(s)	76
BsaAI	YACGTR	1 cut(s)	484
BsaHI	GRCGYC	2 cut(s)	320, 984
BsaI	GGTCTC	1 cut(s)	131
BsaJI	CCNNGG	3 cut(s)	511, 1252, 1309
BsaWI	WCCGGW	2 cut(s)	103, 1080
Bsc4I	CCNNNNNNNGG	2 cut(s)	693, 1266
Bse118I	RCCGGY	4 cut(s)	146, 425, 711, 1080
Bse1I	ACTGG	2 cut(s)	476, 839
Bse21I	CCTNAGG	1 cut(s)	937
Bse3DI	GCAATG	3 cut(s)	681, 949, 1035
BseBI	CCWGG	2 cut(s)	356, 1076
BseCI	ATCGAT	1 cut(s)	76
BseDI	CCNNGG	3 cut(s)	511, 1252, 1309
BseGI	GGATG	4 cut(s)	28, 404, 594, 1410
BseLI	CCNNNNNNNGG	2 cut(s)	693, 1266
BseMI	GCAATG	3 cut(s)	681, 949, 1035
BseMII	CTCAG	3 cut(s)	672, 951, 1364
BseNI	ACTGG	2 cut(s)	476, 839
BseSI	GKGCMC	3 cut(s)	385, 1260, 1309
BseXI	GCAGC	5 cut(s)	130, 346, 647, 784, 892
Bsh1236I	CGCG	1 cut(s)	950
Bsh1285I	CGRYCG	1 cut(s)	954
BshFI	GGCC	9 cut(s)	440, 711, 874, 1186, 1251, 1258, 1307, 1325, 1361
BshTI	ACCGGT	1 cut(s)	1080
BshVI	ATCGAT	1 cut(s)	76
BsiEI	CGRYCG	1 cut(s)	954
BsiHKAI	GWGCWC	2 cut(s)	385, 495
BsiHKCI	CYCGRG	4 cut(s)	510, 563, 1174, 1315
BslFI	GGGAC	3 cut(s)	408, 670, 1535
BslI	CCNNNNNNNGG	2 cut(s)	693, 1266
BsmAI	GTCTC	3 cut(s)	131, 610, 975
BsmBI	CGTCTC	1 cut(s)	975
BsmFI	GGGAC	3 cut(s)	408, 670, 1535
BsmI	GAATGC	1 cut(s)	894
BsnI	GGCC	9 cut(s)	440, 711, 874, 1186, 1251, 1258, 1307, 1325, 1361
Bso31I	GGTCTC	1 cut(s)	131
BsoBI	CYCGRG	4 cut(s)	510, 563, 1174, 1315
Bsp120I	GGGCCC	2 cut(s)	1256, 1305
Bsp1286I	GDGCHC	5 cut(s)	385, 495, 1260, 1309, 1504
Bsp143I	GATC	5 cut(s)	70, 294, 299, 885, 1128
BspACI	CCGC	6 cut(s)	552, 889, 892, 950, 1139, 1229
BspANI	GGCC	9 cut(s)	440, 711, 874, 1186, 1251, 1258, 1307, 1325, 1361
BspCNI	CTCAG	3 cut(s)	673, 950, 1365
BspDI	ATCGAT	1 cut(s)	76
BspFNI	CGCG	1 cut(s)	950
BspHI	TCATGA	2 cut(s)	465, 702
BspLI	GGNNCC	4 cut(s)	6, 1156, 1258, 1307
BspMI	ACCTGC	1 cut(s)	721
BspOI	GCTAGC	1 cut(s)	674
BspPI	GGATC	2 cut(s)	307, 880
BspQI	GCTCTTC	1 cut(s)	1475
BspTNI	GGTCTC	1 cut(s)	131
BsrDI	GCAATG	3 cut(s)	681, 949, 1035
BsrFI	RCCGGY	4 cut(s)	146, 425, 711, 1080
BsrI	ACTGG	2 cut(s)	476, 839
BssAI	RCCGGY	4 cut(s)	146, 425, 711, 1080
BssECI	CCNNGG	3 cut(s)	511, 1252, 1309
BssMI	GATC	5 cut(s)	70, 294, 299, 885, 1128
BssNI	GRCGYC	2 cut(s)	320, 984
BssT1I	CCWWGG	2 cut(s)	1252, 1309
Bst2UI	CCWGG	2 cut(s)	356, 1076
Bst6I	CTCTTC	3 cut(s)	829, 1076, 1475
BstACI	GRCGYC	2 cut(s)	320, 984
BstAPI	GCANNNNNTGC	2 cut(s)	797, 1534
BstBAI	YACGTR	1 cut(s)	484
BstDEI	CTNAG	6 cut(s)	421, 681, 937, 1373, 1486, 1545
BstF5I	GGATG	4 cut(s)	28, 404, 594, 1410
BstFNI	CGCG	1 cut(s)	950
BstHHI	GCGC	2 cut(s)	1204, 1417
BstKTI	GATC	5 cut(s)	73, 297, 302, 888, 1131
BstMAI	GTCTC	3 cut(s)	131, 610, 975
BstMBI	GATC	5 cut(s)	70, 294, 299, 885, 1128
BstMCI	CGRYCG	1 cut(s)	954
BstMWI	GCNNNNNNNGC	7 cut(s)	158, 644, 676, 797, 880, 1119, 1534
BstNI	CCWGG	2 cut(s)	356, 1076
BstNSI	RCATGY	1 cut(s)	815
BstSCI	CCNGG	5 cut(s)	85, 121, 354, 531, 1074
BstSFI	CTRYAG	2 cut(s)	348, 961
BstSLI	GKGCMC	3 cut(s)	385, 1260, 1309
BstSNI	TACGTA	1 cut(s)	484
BstUI	CGCG	1 cut(s)	950
BstV1I	GCAGC	5 cut(s)	130, 346, 647, 784, 892
Bsu15I	ATCGAT	1 cut(s)	76
Bsu36I	CCTNAGG	1 cut(s)	937
BsuRI	GGCC	9 cut(s)	440, 711, 874, 1186, 1251, 1258, 1307, 1325, 1361
BsuTUI	ATCGAT	1 cut(s)	76
BtgZI	GCGATG	1 cut(s)	45
BtsCI	GGATG	4 cut(s)	28, 404, 594, 1410
BveI	ACCTGC	1 cut(s)	721
CciI	TCATGA	2 cut(s)	465, 702
CfoI	GCGC	2 cut(s)	1204, 1417
Cfr10I	RCCGGY	4 cut(s)	146, 425, 711, 1080
Cfr13I	GGNCC	8 cut(s)	19, 873, 1078, 1155, 1256, 1257, 1305, 1306
ClaI	ATCGAT	1 cut(s)	76
CseI	GACGC	3 cut(s)	736, 956, 992
Csp6I	GTAC	4 cut(s)	819, 958, 1084, 1171
CspAI	ACCGGT	1 cut(s)	1080
CspCI	CAANNNNNGTGG	2 cut(s)	833, 868
CviQI	GTAC	4 cut(s)	819, 958, 1084, 1171
DdeI	CTNAG	6 cut(s)	421, 681, 937, 1373, 1486, 1545
DpnI	GATC	5 cut(s)	72, 296, 301, 887, 1130
DpnII	GATC	5 cut(s)	70, 294, 299, 885, 1128
EaeI	YGGCCR	3 cut(s)	438, 1323, 1359
Eam1104I	CTCTTC	3 cut(s)	829, 1076, 1475
EarI	CTCTTC	3 cut(s)	829, 1076, 1475
EciI	GGCGGA	2 cut(s)	567, 878
Ecl136II	GAGCTC	1 cut(s)	493
Eco105I	TACGTA	1 cut(s)	484
Eco130I	CCWWGG	2 cut(s)	1252, 1309
Eco147I	AGGCCT	1 cut(s)	1186
Eco24I	GRGCYC	4 cut(s)	495, 1260, 1309, 1504
Eco31I	GGTCTC	1 cut(s)	131
Eco47I	GGWCC	3 cut(s)	19, 1078, 1155
Eco53kI	GAGCTC	1 cut(s)	493
Eco81I	CCTNAGG	1 cut(s)	937
Eco88I	CYCGRG	4 cut(s)	510, 563, 1174, 1315
EcoICRI	GAGCTC	1 cut(s)	493
EcoO109I	RGGNCCY	1 cut(s)	1305
EcoRI	GAATTC	1 cut(s)	990
EcoRII	CCWGG	2 cut(s)	354, 1074
EcoT14I	CCWWGG	2 cut(s)	1252, 1309
EcoT38I	GRGCYC	4 cut(s)	495, 1260, 1309, 1504
ErhI	CCWWGG	2 cut(s)	1252, 1309
Esp3I	CGTCTC	1 cut(s)	975
FalI	AAGNNNNNCTT	4 cut(s)	699, 731, 1034, 1066
FaqI	GGGAC	3 cut(s)	408, 670, 1535
FblI	GTMKAC	1 cut(s)	430
Fnu4HI	GCNGC	8 cut(s)	119, 360, 636, 798, 892, 906, 1140, 1230
FokI	GGATG	4 cut(s)	15, 391, 581, 1417
FriOI	GRGCYC	4 cut(s)	495, 1260, 1309, 1504
Fsp4HI	GCNGC	8 cut(s)	119, 360, 636, 798, 892, 906, 1140, 1230
FspBI	CTAG	6 cut(s)	398, 671, 690, 816, 866, 1383
GlaI	GCGC	2 cut(s)	1203, 1416
GluI	GCNGC	8 cut(s)	119, 360, 636, 798, 892, 906, 1140, 1230
HaeIII	GGCC	9 cut(s)	440, 711, 874, 1186, 1251, 1258, 1307, 1325, 1361
HgaI	GACGC	3 cut(s)	736, 956, 992
HhaI	GCGC	2 cut(s)	1204, 1417
Hin1I	GRCGYC	2 cut(s)	320, 984
Hin6I	GCGC	2 cut(s)	1202, 1415
HinP1I	GCGC	2 cut(s)	1202, 1415
HincII	GTYRAC	2 cut(s)	366, 919
HindII	GTYRAC	2 cut(s)	366, 919
HinfI	GANTC	5 cut(s)	47, 53, 444, 683, 915
HpaI	GTTAAC	1 cut(s)	366
HphI	GGTGA	4 cut(s)	211, 499, 514, 1357
Hpy166II	GTNNAC	8 cut(s)	83, 366, 383, 431, 481, 919, 958, 1336
Hpy188III	TCNNGA	4 cut(s)	466, 703, 1166, 1243
Hpy8I	GTNNAC	8 cut(s)	83, 366, 383, 431, 481, 919, 958, 1336
Hpy99I	CGWCG	4 cut(s)	325, 730, 1095, 1282
HpyAV	CCTTC	2 cut(s)	10, 418
HpyCH4IV	ACGT	2 cut(s)	320, 483
HpyCH4V	TGCA	6 cut(s)	383, 635, 659, 811, 931, 1421
HpyF10VI	GCNNNNNNNGC	7 cut(s)	158, 644, 676, 797, 880, 1119, 1534
HpyF3I	CTNAG	6 cut(s)	421, 681, 937, 1373, 1486, 1545
HpySE526I	ACGT	2 cut(s)	320, 483
Hsp92I	GRCGYC	2 cut(s)	320, 984
HspAI	GCGC	2 cut(s)	1202, 1415
KroI	GCCGGC	1 cut(s)	711
KroNI	GCCGGC	1 cut(s)	713
KspAI	GTTAAC	1 cut(s)	366
Kzo9I	GATC	5 cut(s)	70, 294, 299, 885, 1128
LguI	GCTCTTC	1 cut(s)	1475
LmnI	GCTCC	2 cut(s)	4, 209
Lsp1109I	GCAGC	5 cut(s)	130, 346, 647, 784, 892
LweI	GCATC	3 cut(s)	603, 1015, 1537
MaeI	CTAG	6 cut(s)	398, 671, 690, 816, 866, 1383
MaeII	ACGT	2 cut(s)	320, 483
MaeIII	GTNAC	3 cut(s)	341, 861, 1018
MalI	GATC	5 cut(s)	72, 296, 301, 887, 1130
MboI	GATC	5 cut(s)	70, 294, 299, 885, 1128
MboII	GAAGA	7 cut(s)	48, 816, 916, 1063, 1231, 1492, 1502
MhlI	GDGCHC	5 cut(s)	385, 495, 1260, 1309, 1504
MlsI	TGGCCA	2 cut(s)	1325, 1361
MluCI	AATT	5 cut(s)	610, 770, 990, 1459, 1494
MluNI	TGGCCA	2 cut(s)	1325, 1361
MlyI	GAGTC	2 cut(s)	692, 924
MmeI	TCCRAC	3 cut(s)	303, 600, 1260
Mox20I	TGGCCA	2 cut(s)	1325, 1361
MroNI	GCCGGC	1 cut(s)	711
MroXI	GAANNNNTTC	1 cut(s)	1287
MscI	TGGCCA	2 cut(s)	1325, 1361
MseI	TTAA	2 cut(s)	365, 374
MslI	CAYNNNNRTG	2 cut(s)	78, 1331
Msp20I	TGGCCA	2 cut(s)	1325, 1361
MspR9I	CCNGG	5 cut(s)	87, 123, 356, 533, 1076
Mva1269I	GAATGC	1 cut(s)	894
MvaI	CCWGG	2 cut(s)	356, 1076
MvnI	CGCG	1 cut(s)	950
MwoI	GCNNNNNNNGC	7 cut(s)	158, 644, 676, 797, 880, 1119, 1534
NaeI	GCCGGC	1 cut(s)	713
NciI	CCSGG	3 cut(s)	87, 123, 533
NdeII	GATC	5 cut(s)	70, 294, 299, 885, 1128
NgoMIV	GCCGGC	1 cut(s)	711
NheI	GCTAGC	1 cut(s)	670
NlaIV	GGNNCC	4 cut(s)	6, 1156, 1258, 1307
NmeAIII	GCCGAG	1 cut(s)	514
NmuCI	GTSAC	1 cut(s)	1018
NspI	RCATGY	1 cut(s)	815
PaeI	GCATGC	1 cut(s)	815
PaeR7I	CTCGAG	2 cut(s)	1174, 1315
PagI	TCATGA	2 cut(s)	465, 702
PceI	AGGCCT	1 cut(s)	1186
PciSI	GCTCTTC	1 cut(s)	1475
PcsI	WCGNNNNNNNCGW	1 cut(s)	1521
PctI	GAATGC	1 cut(s)	894
PdiI	GCCGGC	1 cut(s)	713
PdmI	GAANNNNTTC	1 cut(s)	1287
PfeI	GAWTC	3 cut(s)	47, 53, 444
PflFI	GACNNNGTC	1 cut(s)	950
PflMI	CCANNNNNTGG	1 cut(s)	1266
PfoI	TCCNGGA	2 cut(s)	531, 1074
PinAI	ACCGGT	1 cut(s)	1080
PkrI	GCNGC	8 cut(s)	120, 361, 637, 799, 893, 907, 1141, 1231
PleI	GAGTC	2 cut(s)	691, 923
PpsI	GAGTC	2 cut(s)	691, 923
Ppu21I	YACGTR	1 cut(s)	484
Psp124BI	GAGCTC	1 cut(s)	495
Psp6I	CCWGG	2 cut(s)	354, 1074
PspGI	CCWGG	2 cut(s)	354, 1074
PspN4I	GGNNCC	4 cut(s)	6, 1156, 1258, 1307
PspOMI	GGGCCC	2 cut(s)	1256, 1305
PspPI	GGNCC	8 cut(s)	19, 873, 1078, 1155, 1256, 1257, 1305, 1306
PspXI	VCTCGAGB	2 cut(s)	1174, 1315
PsyI	GACNNNGTC	1 cut(s)	950
RsaI	GTAC	4 cut(s)	820, 959, 1085, 1172
RsaNI	GTAC	4 cut(s)	819, 958, 1084, 1171
RseI	CAYNNNNRTG	2 cut(s)	78, 1331
SacI	GAGCTC	1 cut(s)	495
SapI	GCTCTTC	1 cut(s)	1475
SaqAI	TTAA	2 cut(s)	365, 374
SatI	GCNGC	8 cut(s)	119, 360, 636, 798, 892, 906, 1140, 1230
Sau3AI	GATC	5 cut(s)	70, 294, 299, 885, 1128
Sau96I	GGNCC	8 cut(s)	19, 873, 1078, 1155, 1256, 1257, 1305, 1306
ScaI	AGTACT	1 cut(s)	1172
SchI	GAGTC	2 cut(s)	692, 924
ScrFI	CCNGG	5 cut(s)	87, 123, 356, 533, 1076
SduI	GDGCHC	5 cut(s)	385, 495, 1260, 1309, 1504
SfaNI	GCATC	3 cut(s)	603, 1015, 1537
SfcI	CTRYAG	2 cut(s)	348, 961
Sfr274I	CTCGAG	2 cut(s)	1174, 1315
SinI	GGWCC	3 cut(s)	19, 1078, 1155
SlaI	CTCGAG	2 cut(s)	1174, 1315
SmiMI	CAYNNNNRTG	2 cut(s)	78, 1331
SmlI	CTYRAG	3 cut(s)	1166, 1174, 1315
SmoI	CTYRAG	3 cut(s)	1166, 1174, 1315
SnaBI	TACGTA	1 cut(s)	484
SpeI	ACTAGT	1 cut(s)	865
SphI	GCATGC	1 cut(s)	815
Sse9I	AATT	5 cut(s)	610, 770, 990, 1459, 1494
SseBI	AGGCCT	1 cut(s)	1186
SsiI	CCGC	6 cut(s)	552, 889, 892, 950, 1139, 1229
SspI	AATATT	1 cut(s)	1287
SspMI	CTAG	6 cut(s)	398, 671, 690, 816, 866, 1383
SstI	GAGCTC	1 cut(s)	495
StuI	AGGCCT	1 cut(s)	1186
StyD4I	CCNGG	5 cut(s)	85, 121, 354, 531, 1074
StyI	CCWWGG	2 cut(s)	1252, 1309
TaiI	ACGT	2 cut(s)	323, 486
TaqI	TCGA	4 cut(s)	76, 1175, 1244, 1316
TasI	AATT	5 cut(s)	610, 770, 990, 1459, 1494
TatI	WGTACW	2 cut(s)	957, 1170
TauI	GCSGC	3 cut(s)	894, 1142, 1232
TfiI	GAWTC	3 cut(s)	47, 53, 444
Tru1I	TTAA	2 cut(s)	365, 374
Tru9I	TTAA	2 cut(s)	365, 374
TseFI	GTSAC	1 cut(s)	1018
TseI	GCWGC	5 cut(s)	118, 359, 635, 797, 905
Tsp45I	GTSAC	1 cut(s)	1018
TspDTI	ATGAA	8 cut(s)	48, 101, 234, 279, 532, 623, 719, 916
TspGWI	ACGGA	1 cut(s)	1101
Tth111I	GACNNNGTC	1 cut(s)	950
Van91I	CCANNNNNTGG	1 cut(s)	1266
VneI	GTGCAC	1 cut(s)	381
VpaK11BI	GGWCC	3 cut(s)	19, 1078, 1155
XapI	RAATTY	1 cut(s)	990
XceI	RCATGY	1 cut(s)	815
XhoI	CTCGAG	2 cut(s)	1174, 1315
XmiI	GTMKAC	1 cut(s)	430
XmnI	GAANNNNTTC	1 cut(s)	1287
XspI	CTAG	6 cut(s)	398, 671, 690, 816, 866, 1383
ZraI	GACGTC	1 cut(s)	321
ZrmI	AGTACT	1 cut(s)	1172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.