MD02G1048000.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016725
Physical Location & Seq
Reverse (-)
3801478 .. 3803884
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1048000.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGGAACTGGGAAAACAAGTTTGGTACTGAGATTTGTGACTGGAAAATTTTTGGAGTACCAGGAATCAACAATTGGAGCAGCTTTCTTCACTCAGGTTCTGTCACTAAATGAAGCTACTATCAAATTCGATATATGGGACACCGCCGGCCAGGAAAGGTACCACAGCCTGGCTCCTATGTACTACCGCGGCGCTGCTGCGGCTGTCGTGGTGTATGATATCACAAGCATGGAGTCATTCGTCCGAGCAAAAAAATGGGTTCAAGAATTGCAACGACAAGCAAATCCAACATTAATAATGTTCTTGGCTGGTAACAAGGCTGACTTGGAAGAGAAGAGAAAAGTAGGGTCTGAGGAAGCTGAGCAATATGCTAAGGAAAATGGCTTGGTTTTTCTTGAAACGTCTGCAAAAACTGCACAGAATGTCAGTGAGCTCTTTTATGAAATAGCAAAAAAAGTGGCCAAAGCTAGCCCTTCTCGTCAAACTGGGATAAAATTACACATCAGATCACAGCCACCCAGCAGAAGAATGTTTTGTTGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04144 ko04145 ko04962 ko05146 ko05152 map04014 map04144 map04145 map04962 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

182

Amino Acids

20.35

Weight (kDa)

9.18

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 2 - 154 7.5e-56 Ras family
Roc PF08477 2 - 108 1.7e-29 Ras of Complex, Roc, domain of DAPkinase
Arf PF00025 3 - 147 4.5e-10 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04940 FvH4_1g04940 FvH4_1g04940 FvH4_1g04940
malus_domestica MD02G1048000.v1.1
prunus_persica Prupe.7G230200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0090541 RchiOBHm_Chr2g0111381
rosa_laevigata RLG00000016121
rosa_multiflora Rmu_sc0004514.1_g000028
rosa_roxburghii Rroxscaffold_2G00151040
rosa_rugosa Rorug02G0008900 Rorug02G0009000
rosa_wichuraiana Rw2G004760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 159
AccB7I CCANNNNNTGG 1 cut(s) 169
AccII CGCG 1 cut(s) 189
AciI CCGC 4 cut(s) 144, 187, 189, 200
AcoI YGGCCR 2 cut(s) 148, 459
AcsI RAATTY 2 cut(s) 47, 125
AfaI GTAC 4 cut(s) 27, 59, 161, 182
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 263, 398
AjnI CCWGG 3 cut(s) 60, 150, 168
AjuI GAANNNNNNNTTGG 3 cut(s) 37, 57, 89
AluBI AGCT 5 cut(s) 83, 116, 359, 433, 467
AluI AGCT 5 cut(s) 83, 116, 359, 433, 467
Alw21I GWGCWC 1 cut(s) 435
AlwNI CAGNNNCTG 1 cut(s) 100
AoxI GGCC 2 cut(s) 148, 459
ApeKI GCWGC 3 cut(s) 80, 194, 197
ApoI RAATTY 2 cut(s) 47, 125
AseI ATTAAT 1 cut(s) 293
Asp718I GGTACC 1 cut(s) 159
AspLEI GCGC 1 cut(s) 194
AsuNHI GCTAGC 1 cut(s) 467
BalI TGGCCA 1 cut(s) 461
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 92, 181, 184
BciT130I CCWGG 3 cut(s) 62, 152, 170
BfaI CTAG 1 cut(s) 468
BfoI RGCGCY 1 cut(s) 195
BisI GCNGC 5 cut(s) 81, 190, 195, 198, 201
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 5 cut(s) 82, 191, 196, 199, 202
Bme1390I CCNGG 3 cut(s) 62, 152, 170
BmiI GGNNCC 2 cut(s) 161, 174
BmrFI CCNGG 3 cut(s) 62, 152, 170
BmrI ACTGGG 2 cut(s) 18, 495
BmtI GCTAGC 1 cut(s) 471
BmuI ACTGGG 2 cut(s) 18, 495
Bpu10I CCTNAGC 1 cut(s) 372
Bpu1102I GCTNAGC 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 3 cut(s) 13, 46, 490
BseBI CCWGG 3 cut(s) 62, 152, 170
BseDI CCNNGG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMII CTCAG 4 cut(s) 20, 107, 342, 351
BseNI ACTGG 3 cut(s) 13, 46, 490
BseXI GCAGC 3 cut(s) 92, 181, 184
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 150, 461
BshNI GGYRCC 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 435
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 2 cut(s) 150, 461
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 1 cut(s) 506
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 4 cut(s) 144, 187, 189, 200
BspANI GGCC 2 cut(s) 150, 461
BspCNI CTCAG 4 cut(s) 21, 106, 343, 352
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 161, 174
BspOI GCTAGC 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 3 cut(s) 13, 46, 490
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 1 cut(s) 506
Bst2UI CCWGG 3 cut(s) 62, 152, 170
Bst6I CTCTTC 2 cut(s) 324, 329
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 148, 469
BstDEI CTNAG 5 cut(s) 29, 93, 351, 360, 372
BstDSI CCRYGG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 1 cut(s) 509
BstMBI GATC 1 cut(s) 506
BstMWI GCNNNNNNNGC 2 cut(s) 200, 413
BstNI CCWGG 3 cut(s) 62, 152, 170
BstSCI CCNGG 3 cut(s) 60, 150, 168
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 92, 181, 184
BsuRI GGCC 2 cut(s) 150, 461
BtgI CCRYGG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 148, 469
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 146
Cfr42I CCGCGG 1 cut(s) 190
Csp6I GTAC 4 cut(s) 26, 58, 160, 181
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 1 cut(s) 229
CviQI GTAC 4 cut(s) 26, 58, 160, 181
DdeI CTNAG 5 cut(s) 29, 93, 351, 360, 372
DpnI GATC 1 cut(s) 508
DpnII GATC 1 cut(s) 506
EaeI YGGCCR 2 cut(s) 148, 459
Eam1104I CTCTTC 2 cut(s) 324, 329
EarI CTCTTC 2 cut(s) 324, 329
Ecl136II GAGCTC 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 433
EcoICRI GAGCTC 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 60, 150, 168
EcoRV GATATC 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 435
FaeI CATG 1 cut(s) 232
FaiI YATR 7 cut(s) 134, 136, 179, 216, 230, 369, 441
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 1 cut(s) 152
FatI CATG 1 cut(s) 228
Fnu4HI GCNGC 5 cut(s) 81, 190, 195, 198, 201
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 5 cut(s) 81, 190, 195, 198, 201
FspBI CTAG 1 cut(s) 468
GlaI GCGC 1 cut(s) 193
GluI GCNGC 5 cut(s) 81, 190, 195, 198, 201
GsaI CCCAGC 1 cut(s) 522
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 2 cut(s) 150, 461
HapII CCGG 1 cut(s) 147
HhaI GCGC 1 cut(s) 194
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 2 cut(s) 65, 233
HpaII CCGG 1 cut(s) 147
Hpy188I TCNGA 3 cut(s) 245, 352, 506
Hpy188III TCNNGA 2 cut(s) 263, 395
HpyAV CCTTC 1 cut(s) 483
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 4 cut(s) 271, 407, 416, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 413
HpyF3I CTNAG 5 cut(s) 29, 93, 351, 360, 372
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 163
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
KspI CCGCGG 1 cut(s) 190
Kzo9I GATC 1 cut(s) 506
LmnI GCTCC 2 cut(s) 77, 178
Lsp1109I GCAGC 3 cut(s) 92, 181, 184
MaeI CTAG 1 cut(s) 468
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 3 cut(s) 37, 102, 311
MalI GATC 1 cut(s) 508
MboI GATC 1 cut(s) 506
MboII GAAGA 4 cut(s) 79, 341, 346, 537
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 435
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 5 cut(s) 47, 72, 125, 266, 494
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 1 cut(s) 346
Mox20I TGGCCA 1 cut(s) 461
MroNI GCCGGC 1 cut(s) 146
MscI TGGCCA 1 cut(s) 461
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 227
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 3 cut(s) 62, 152, 170
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 3 cut(s) 62, 152, 170
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 200, 413
NaeI GCCGGC 1 cut(s) 148
NdeII GATC 1 cut(s) 506
NgoMIV GCCGGC 1 cut(s) 146
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 2 cut(s) 161, 174
NmuCI GTSAC 2 cut(s) 37, 102
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 5 cut(s) 82, 191, 196, 199, 202
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PshBI ATTAAT 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 60, 150, 168
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 3 cut(s) 60, 150, 168
PspN4I GGNNCC 2 cut(s) 161, 174
PstNI CAGNNNCTG 1 cut(s) 100
RsaI GTAC 4 cut(s) 27, 59, 161, 182
RsaNI GTAC 4 cut(s) 26, 58, 160, 181
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 435
SacII CCGCGG 1 cut(s) 190
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 5 cut(s) 81, 190, 195, 198, 201
Sau3AI GATC 1 cut(s) 506
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 3 cut(s) 62, 152, 170
SduI GDGCHC 1 cut(s) 435
SetI ASST 8 cut(s) 85, 99, 118, 161, 361, 404, 435, 469
Sfr303I CCGCGG 1 cut(s) 190
SgrBI CCGCGG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 5 cut(s) 47, 72, 125, 266, 494
SsiI CCGC 4 cut(s) 144, 187, 189, 200
SspMI CTAG 1 cut(s) 468
SstI GAGCTC 1 cut(s) 435
StyD4I CCNGG 3 cut(s) 60, 150, 168
TaiI ACGT 1 cut(s) 404
TaqI TCGA 1 cut(s) 129
TasI AATT 5 cut(s) 47, 72, 125, 266, 494
TatI WGTACW 1 cut(s) 180
TauI GCSGC 2 cut(s) 192, 203
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 2 cut(s) 37, 102
TseI GCWGC 3 cut(s) 80, 194, 197
Tsp45I GTSAC 2 cut(s) 37, 102
TspDTI ATGAA 2 cut(s) 126, 456
TspRI CASTG 1 cut(s) 433
Van91I CCANNNNNTGG 1 cut(s) 169
VspI ATTAAT 1 cut(s) 293
XapI RAATTY 2 cut(s) 47, 125
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.