MD02G1048100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017570
Physical Location & Seq
Forward (+)
3807721 .. 3808236
516 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1048100.v1.1.491

Sequence Viewer

Length: 249 bp
ATGGCTTCCCATGGCAATGGTCTGAAAGTCTCAGTTTCTCCAAAAAATGGTGGCAACGCAGAAGCTGCATCACTTACTTCACCAAAAGGCCAATGTCTGTGCTCACCAACGACACATCAAGGCTCTTTCCGGTGCCGTTTTCACAGGTCCCGGTCATCGACTTGGATGAAGCGATCAAAGTCCATGCCTAGCAATAATAACTCAGTGGCTTCTTTCTCTCCCACATCAGTACTTGATCAGTCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

83

Amino Acids

8.7

Weight (kDa)

10.55

Isoelectric Point (pI)

64.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017570)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04950
malus_domestica MD02G1048100.v1.1
prunus_persica Prupe.7G230000_v2.0.a1
pyrus_communis pycom02g04020 pycom15g16720
rosa_chinensis RchiOBHm_Chr2g0090561
rosa_roxburghii Rroxscaffold_2G00151030
rosa_rugosa Rorug02G0009100
rosa_samantha Rh2BG053600 Rh2CG055800 Rh2DG054700
rosa_wichuraiana Rw2G004750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 132
AccB7I CCANNNNNTGG 1 cut(s) 47
AccI GTMKAC 1 cut(s) 242
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 47
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 34
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 147
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 2 cut(s) 72, 96
AvaII GGWCC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 52
BceAI ACGGC 1 cut(s) 120
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 1 cut(s) 189
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
BmcAI AGTACT 1 cut(s) 231
Bme1390I CCNGG 1 cut(s) 151
Bme18I GGWCC 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 2 cut(s) 134, 149
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse3DI GCAATG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 22
BseMII CTCAG 2 cut(s) 45, 216
BseXI GCAGC 1 cut(s) 52
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 2 cut(s) 130, 151
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 2 cut(s) 173, 235
Bsp19I CCATGG 1 cut(s) 10
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 2 cut(s) 44, 215
BspLI GGNNCC 2 cut(s) 134, 149
BspT107I GGYRCC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 173, 235
BssT1I CCWWGG 1 cut(s) 10
BstAPI GCANNNNNTGC 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 31, 202
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 1 cut(s) 171
BstKTI GATC 2 cut(s) 176, 238
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 2 cut(s) 173, 235
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 17
BsuRI GGCC 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 210
CaiI CAGNNNCTG 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 147
Csp6I GTAC 1 cut(s) 230
CviAII CATG 2 cut(s) 11, 184
CviJI RGCY 5 cut(s) 5, 65, 90, 123, 209
CviKI_1 RGCY 5 cut(s) 5, 65, 90, 123, 209
CviQI GTAC 1 cut(s) 230
DdeI CTNAG 2 cut(s) 31, 202
DpnI GATC 2 cut(s) 175, 237
DpnII GATC 2 cut(s) 173, 235
Eco130I CCWWGG 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 2 cut(s) 14, 187
FaiI YATR 2 cut(s) 12, 185
FaqI GGGAC 1 cut(s) 133
FatI CATG 2 cut(s) 10, 183
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 242
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 189
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 90
HapII CCGG 2 cut(s) 130, 151
Hin1II CATG 2 cut(s) 14, 187
HpaII CCGG 2 cut(s) 130, 151
HphI GGTGA 2 cut(s) 72, 96
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 1 cut(s) 24
Hpy8I GTNNAC 1 cut(s) 243
HpyCH4V TGCA 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 2 cut(s) 31, 202
Hsp92II CATG 2 cut(s) 14, 187
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 2 cut(s) 173, 235
LpnPI CCDG 3 cut(s) 130, 143, 164
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 1 cut(s) 77
MaeI CTAG 1 cut(s) 189
MalI GATC 2 cut(s) 175, 237
MboI GATC 2 cut(s) 173, 235
MhlI GDGCHC 1 cut(s) 104
MseI TTAA 1 cut(s) 247
MslI CAYNNNNRTG 1 cut(s) 15
MspI CCGG 2 cut(s) 130, 151
MspR9I CCNGG 1 cut(s) 151
MwoI GCNNNNNNNGC 1 cut(s) 65
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 10
NdeII GATC 2 cut(s) 173, 235
NlaIII CATG 2 cut(s) 14, 187
NlaIV GGNNCC 2 cut(s) 134, 149
PflMI CCANNNNNTGG 1 cut(s) 47
PkrI GCNGC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 134, 149
PspPI GGNCC 1 cut(s) 147
PspPPI RGGWCCY 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 247
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 2 cut(s) 173, 235
Sau96I GGNCC 1 cut(s) 147
ScaI AGTACT 1 cut(s) 231
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 104
SetI ASST 2 cut(s) 67, 149
SfaNI GCATC 1 cut(s) 77
SinI GGWCC 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 15
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 1 cut(s) 10
TaqI TCGA 1 cut(s) 158
TatI WGTACW 1 cut(s) 229
Tru1I TTAA 1 cut(s) 247
Tru9I TTAA 1 cut(s) 247
TscAI CASTG 1 cut(s) 210
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 182
TspRI CASTG 1 cut(s) 210
Van91I CCANNNNNTGG 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 242
XspI CTAG 1 cut(s) 189
ZrmI AGTACT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.