MD02G1048800.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012557
Physical Location & Seq
Forward (+)
3838656 .. 3844418
5763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1048800.v1.1.491

Sequence Viewer

Length: 2034 bp
ATGCCAGATTTAAGAAGTGGAGTTCGACGTTCAAAGAGGATCAATTATGTCCAGGAAGATCCTGCTGTTCTAGTTCCAGCTGTGCGGCCTGGTGCTGGAAAAGTTCTGACTGTTAAAGGTAGGGGTAGAGGGTCTAGAGCCCTGAATCAAGTTAAGAATTCCAAGCTATTTGGTGCTGGGGTTGGTGGCCAAGGGCATACAGGTTTGGGCTTTCCAGAAAGAGAATTAGCGCCGGTTCACGACGAGTTGGTAGATCAGAAAGGTGCACAGAAATTGGTTGCTATGGAGGATGAAGGAAGCACTAGTCCACTTCCGGACAGAGTACAAGTTGGCAAATCTCCCTTGTACAAGCTAGACAGAAAGTTGGGGAAAGGGGGTTTTGGACAAGTTTATGTGGGAAGAAGGATGACTGGCGGCTTAGGGTGCTCTGGGGCTGATGCCTATGAGGTTGCTCTGAAGCTTGAAAGTAAAAATGGTAAAGGTTGCAGCCACGGCCCTCCTTATGAGTGGCAAGTATACAGTACTCTTAATGGATGCTATGGGCTTCCCCTCGTCCATTACAAAGGTCAACAGGGAGAGTACTATATCCTAGTCATGGATATGCTAGGCCCAAGTTTATGGGATGTTTGGAATTCCCGCAATCAGTTGCTGTCTGAAGAGATGGTTGCTTGTATAGCAGTGGAGGCAATCTCTATTCTGCAGCAGCTTCACACTAGAGGTTTTGTGCATGGAGATGTTAAGCCAGAAAACTTTTTACTTGGTCTACCTGGAACACCTAATGAGAAGAAGTTATATCTTGTTGATCTTGGTTTGGCTTCCAGATGGAGAGATGGGTCTTCTGGACGTCATGTTGATTATGACCAAAAACCTGATGTTTTCAGGGGAACTGTACGTTATGCAAGTGTACACGCTCATTTAGGAAGGACAGGGAGCCGAAGGGATGACCTTGAATCGTTAGCTTATACCCTAATATTTCTCCTTAGAGGAAAGCTTCCCTGGCAAGGCTACAATGGAGAGAACAAAGGTTTTCTTGTTTGCAAGAAGAAGATGGCAACTTCTGCTGAGACGCTATGCAGCTTATGCCCTCCCCCTTTTCAACAGTTTCTTGAGATGGTTACCAATATGAAATTTGATGAAGAACCAAATTACTTGAAGCTTATTTCCCTTTTTGATAACAGCATTGGCACAATTGCCTCTCTGCGACCTATCAGAACTGATGGAGCTGCAAAGGCGGGTCAAAAAAGGGGAAGATTGCCTGTTGACTCAGTAGATGATGAGCAGCCTAAGAAAAAAGTTCGCCAAGGTAGTCCAGCTACTCAGTGGATCTCGGTCTATAATACCAGATCATCAATGAAACAAAGGTATCACTACAATGTTTTGGATCAAAGACTGGACCAGCACATTGATAAAGGAAAGGAAGATGGTTTGTATATTAGTTGTGTCGCTTCATCTGTGAATATGTGGGCTATCGTCATGGATGCTGGGACTGGCTTCACATCCCAAGTTTATGAACGATCATCTATTTTCTTGCACAAGGAATGGATAATGGAACAATGGGACAAGAACTACTATATTACTTCACTTGCTGGCACATTAAATGGCAGTGCTCTGGTGGTAATGTCCCAAGGTGTTCCTTATACTCAACAATCCTACAAAGTCAGTGATGTGTTCCCTTTCAAATGGATTAATAAGAAGTGGAAAGAAGGTTTCTCTGTTACCTCCATGACTACTGCAGGTAGCAGATGGGGTATTGTCATGTCCCGAAATGCAGGTTATTCTGGTCAGGTTGTCGAACTTGATTTCCTGTATCCCAGTGAAGGAATCCATAGAAGATGGGAGAACGGATATCGGATTACCTCCACTGCGGCTACGGAGGATCAAGCTGCATTCATACTTAGTGTACCCAAGAAGAAATCACCAGATGTGGCTCAAGAAACTCTGCGGACATCGATTTTTCCAACTTACCAAGTGAAGGAAAAATGGTTAAAGAATCTCTACATTGCATCTATCTGCTACGGGAGAACGGTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007165 GO:0007166 GO:0007267 GO:0007346 GO:0008150 GO:0008152 GO:0008360 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010564 GO:0010604 GO:0015630 GO:0016055 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019222 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032879 GO:0032886 GO:0033043 GO:0035567 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051493 GO:0051716 GO:0051726 GO:0060069 GO:0060255 GO:0060341 GO:0061136 GO:0065007 GO:0065008 GO:0070507 GO:0071704 GO:0080090 GO:0098657 GO:0140096 GO:0198738 GO:1901564 GO:1901800 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1905114 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

678

Amino Acids

75.5

Weight (kDa)

9.3

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 116 - 348 1.6e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 117 - 277 8.6e-07 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 220 - 325 3.4e-07 Fungal protein kinase
DUF7477 PF24289 410 - 677 2.6e-151 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05050 FvH4_1g05050 FvH4_1g05050
malus_domestica MD02G1048800.v1.1 MD15G1187100.v1.1
prunus_persica Prupe.7G229400_v2.0.a1
pyrus_communis pycom02g04090 pycom15g16760
rosa_chinensis RchiOBHm_Chr2g0090661
rosa_laevigata RLG00000016134
rosa_multiflora Rmu_sc0016852.1_g000008
rosa_roxburghii Rroxscaffold_2G00150900
rosa_rugosa Rorug02G0009900 Rorug02G0009900
rosa_samantha Rh2AG055800 Rh2BG054200 Rh2CG056500 Rh2DG055100
rosa_wichuraiana Rw2G004700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 847
Acc36I ACCTGC 2 cut(s) 1724, 1760
AccI GTMKAC 2 cut(s) 516, 763
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 6 cut(s) 85, 414, 637, 1232, 1865, 1942
AclWI GGATC 5 cut(s) 47, 53, 1331, 1389, 1884
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 3 cut(s) 157, 631, 1127
AcuI CTGAAG 2 cut(s) 476, 675
AcyI GRCGYC 1 cut(s) 844
AfaI GTAC 7 cut(s) 324, 347, 523, 581, 891, 906, 1902
AfiI CCNNNNNNNGG 5 cut(s) 95, 595, 1001, 1817, 1972
AgsI TTSAA 6 cut(s) 33, 464, 950, 1097, 1153, 1678
AhlI ACTAGT 1 cut(s) 302
AjnI CCWGG 4 cut(s) 51, 88, 766, 995
AloI GAACNNNNNNTCC 2 cut(s) 1785, 1817
Alw21I GWGCWC 3 cut(s) 268, 428, 1609
Alw26I GTCTC 1 cut(s) 1058
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 5 cut(s) 47, 53, 1331, 1389, 1884
AlwNI CAGNNNCTG 1 cut(s) 649
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 4 cut(s) 86, 187, 493, 607
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 7 cut(s) 486, 700, 703, 1074, 1223, 1279, 1883
ApoI RAATTY 3 cut(s) 157, 631, 1127
AseI ATTAAT 1 cut(s) 1686
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 494, 608, 1393
AsuHPI GGTGA 1 cut(s) 1908
AvaII GGWCC 1 cut(s) 1393
BaeGI GKGCMC 1 cut(s) 268
BaeI ACNNNNGTAYC 2 cut(s) 1346, 1379
BalI TGGCCA 1 cut(s) 189
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 3 cut(s) 268, 428, 1609
BbvI GCAGC 7 cut(s) 498, 712, 715, 1086, 1210, 1291, 1870
BccI CCATC 9 cut(s) 655, 816, 824, 1042, 1105, 1211, 1415, 1737, 1827
BceAI ACGGC 1 cut(s) 508
BciT130I CCWGG 4 cut(s) 53, 90, 768, 997
BciVI GTATCC 1 cut(s) 1818
BcoDI GTCTC 1 cut(s) 1058
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 7 cut(s) 71, 135, 303, 353, 590, 605, 714
BfmI CTRYAG 2 cut(s) 698, 1731
BfoI RGCGCY 1 cut(s) 233
BfuAI ACCTGC 2 cut(s) 1724, 1760
BfuI GTATCC 1 cut(s) 1818
BmcAI AGTACT 2 cut(s) 523, 581
Bme1390I CCNGG 4 cut(s) 53, 90, 768, 997
Bme18I GGWCC 1 cut(s) 1393
BmgT120I GGNCC 3 cut(s) 494, 608, 1393
BmiI GGNNCC 1 cut(s) 932
BmrFI CCNGG 4 cut(s) 53, 90, 768, 997
BmrI ACTGGG 1 cut(s) 1806
BmsI GCATC 4 cut(s) 427, 524, 1468, 2012
BmuI ACTGGG 1 cut(s) 1806
BpiI GAAGAC 1 cut(s) 828
BplI GAGNNNNNCTC 2 cut(s) 674, 706
Bpu10I CCTNAGC 1 cut(s) 418
BpuEI CTTGAG 2 cut(s) 1127, 1914
Bsa29I ATCGAT 1 cut(s) 1949
BsaHI GRCGYC 1 cut(s) 844
BsaJI CCNNGG 5 cut(s) 190, 490, 995, 1300, 1624
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 595, 1001, 1817, 1972
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 4 cut(s) 415, 1395, 1492, 1812
Bse3DI GCAATG 1 cut(s) 1998
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 4 cut(s) 53, 90, 768, 997
BseCI ATCGAT 1 cut(s) 1949
BseDI CCNNGG 5 cut(s) 190, 490, 995, 1300, 1624
BseGI GGATG 7 cut(s) 295, 411, 539, 628, 946, 1483, 1496
BseLI CCNNNNNNNGG 5 cut(s) 95, 595, 1001, 1817, 1972
BseMI GCAATG 1 cut(s) 1998
BseMII CTCAG 3 cut(s) 1053, 1278, 1331
BseNI ACTGG 4 cut(s) 415, 1395, 1492, 1812
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 7 cut(s) 498, 712, 715, 1086, 1210, 1291, 1870
BseYI CCCAGC 2 cut(s) 176, 1481
BshFI GGCC 4 cut(s) 88, 189, 495, 609
BshVI ATCGAT 1 cut(s) 1949
BsiHKAI GWGCWC 3 cut(s) 268, 428, 1609
BsiSI CCGG 2 cut(s) 233, 314
BslFI GGGAC 4 cut(s) 1498, 1571, 1606, 1744
BslI CCNNNNNNNGG 5 cut(s) 95, 595, 1001, 1817, 1972
BsmAI GTCTC 1 cut(s) 1058
BsmBI CGTCTC 1 cut(s) 1058
BsmFI GGGAC 4 cut(s) 1498, 1571, 1606, 1744
BsmI GAATGC 1 cut(s) 1886
BsnI GGCC 4 cut(s) 88, 189, 495, 609
Bsp1286I GDGCHC 4 cut(s) 142, 268, 428, 1609
Bsp13I TCCGGA 1 cut(s) 313
Bsp1407I TGTACA 2 cut(s) 345, 904
Bsp143I GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
BspACI CCGC 6 cut(s) 85, 414, 637, 1232, 1865, 1942
BspANI GGCC 4 cut(s) 88, 189, 495, 609
BspCNI CTCAG 3 cut(s) 1054, 1277, 1330
BspDI ATCGAT 1 cut(s) 1949
BspEI TCCGGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 932
BspMAI CTGCAG 2 cut(s) 702, 1735
BspMI ACCTGC 2 cut(s) 1724, 1760
BspPI GGATC 5 cut(s) 47, 53, 1331, 1389, 1884
BsrDI GCAATG 1 cut(s) 1998
BsrFI RCCGGY 1 cut(s) 232
BsrGI TGTACA 2 cut(s) 345, 904
BsrI ACTGG 4 cut(s) 415, 1395, 1492, 1812
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 5 cut(s) 190, 490, 995, 1300, 1624
BssMI GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
BssNAI GTATAC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 844
BssT1I CCWWGG 3 cut(s) 190, 1300, 1624
Bst1107I GTATAC 1 cut(s) 517
Bst2UI CCWGG 4 cut(s) 53, 90, 768, 997
Bst4CI ACNGT 5 cut(s) 112, 521, 889, 1101, 2026
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 1 cut(s) 844
BstAPI GCANNNNNTGC 2 cut(s) 1058, 1080
BstAUI TGTACA 2 cut(s) 345, 904
BstC8I GCNNGC 1 cut(s) 1588
BstDEI CTNAG 7 cut(s) 418, 980, 1062, 1264, 1284, 1317, 1895
BstDSI CCRYGG 1 cut(s) 490
BstEII GGTNACC 1 cut(s) 1114
BstF5I GGATG 7 cut(s) 295, 411, 539, 628, 946, 1483, 1496
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 9 cut(s) 42, 61, 256, 805, 1326, 1346, 1384, 1517, 1879
BstMAI GTCTC 1 cut(s) 1058
BstMBI GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
BstMWI GCNNNNNNNGC 8 cut(s) 423, 492, 674, 683, 997, 1058, 1080, 1229
BstNI CCWGG 4 cut(s) 53, 90, 768, 997
BstPI GGTNACC 1 cut(s) 1114
BstSCI CCNGG 4 cut(s) 51, 88, 766, 995
BstSFI CTRYAG 2 cut(s) 698, 1731
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 7 cut(s) 498, 712, 715, 1086, 1210, 1291, 1870
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 2 cut(s) 58, 1323
BstXI CCANNNNNNTGG 1 cut(s) 618
BstYI RGATCY 2 cut(s) 58, 1323
BstZ17I GTATAC 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 1949
BsuI GTATCC 1 cut(s) 1818
BsuRI GGCC 4 cut(s) 88, 189, 495, 609
BsuTUI ATCGAT 1 cut(s) 1949
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 7 cut(s) 295, 411, 539, 628, 946, 1483, 1496
BtsI GCAGTG 3 cut(s) 684, 1609, 1860
BtsIMutI CAGTG 6 cut(s) 684, 1325, 1609, 1666, 1819, 1860
BveI ACCTGC 2 cut(s) 1724, 1760
Cac8I GCNNGC 1 cut(s) 1588
CaiI CAGNNNCTG 1 cut(s) 649
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 494, 608, 1393
ClaI ATCGAT 1 cut(s) 1949
CseI GACGC 1 cut(s) 1075
Csp6I GTAC 7 cut(s) 323, 346, 522, 580, 890, 905, 1901
CviAII CATG 6 cut(s) 595, 728, 848, 1474, 1723, 1756
CviQI GTAC 7 cut(s) 323, 346, 522, 580, 890, 905, 1901
DdeI CTNAG 7 cut(s) 418, 980, 1062, 1264, 1284, 1317, 1895
DpnI GATC 9 cut(s) 41, 60, 255, 804, 1325, 1345, 1383, 1516, 1878
DpnII GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Eco130I CCWWGG 3 cut(s) 190, 1300, 1624
Eco24I GRGCYC 1 cut(s) 142
Eco32I GATATC 1 cut(s) 1847
Eco47I GGWCC 1 cut(s) 1393
Eco57I CTGAAG 2 cut(s) 476, 675
Eco91I GGTNACC 1 cut(s) 1114
EcoO65I GGTNACC 1 cut(s) 1114
EcoRI GAATTC 2 cut(s) 157, 631
EcoRII CCWGG 4 cut(s) 51, 88, 766, 995
EcoRV GATATC 1 cut(s) 1847
EcoT14I CCWWGG 3 cut(s) 190, 1300, 1624
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 3 cut(s) 190, 1300, 1624
Esp3I CGTCTC 1 cut(s) 1058
FaeI CATG 6 cut(s) 598, 731, 851, 1477, 1726, 1759
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 4 cut(s) 1498, 1571, 1606, 1744
FatI CATG 6 cut(s) 594, 727, 847, 1473, 1722, 1755
FauI CCCGC 2 cut(s) 644, 1225
FblI GTMKAC 2 cut(s) 516, 763
FokI GGATG 7 cut(s) 302, 418, 546, 635, 953, 1483, 1490
FriOI GRGCYC 1 cut(s) 142
FspBI CTAG 7 cut(s) 71, 135, 303, 353, 590, 605, 714
GlaI GCGC 1 cut(s) 231
GsaI CCCAGC 2 cut(s) 180, 1485
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 4 cut(s) 88, 189, 495, 609
HapII CCGG 2 cut(s) 233, 314
HgaI GACGC 1 cut(s) 1075
HhaI GCGC 1 cut(s) 232
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 6 cut(s) 598, 731, 851, 1477, 1726, 1759
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 2 cut(s) 569, 1261
HindII GTYRAC 2 cut(s) 569, 1261
HindIII AAGCTT 3 cut(s) 458, 989, 1154
HinfI GANTC 5 cut(s) 145, 950, 1262, 1821, 1990
HpaII CCGG 2 cut(s) 233, 314
HphI GGTGA 1 cut(s) 1908
Hpy188I TCNGA 6 cut(s) 108, 258, 456, 655, 1211, 1851
Hpy99I CGWCG 2 cut(s) 30, 245
HpyAV CCTTC 7 cut(s) 287, 396, 915, 930, 1697, 1811, 1966
HpyCH4III ACNGT 5 cut(s) 112, 521, 889, 1101, 2026
HpyCH4IV ACGT 3 cut(s) 28, 844, 892
HpyF10VI GCNNNNNNNGC 8 cut(s) 423, 492, 674, 683, 997, 1058, 1080, 1229
HpyF3I CTNAG 7 cut(s) 418, 980, 1062, 1264, 1284, 1317, 1895
HpySE526I ACGT 3 cut(s) 28, 844, 892
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 6 cut(s) 598, 731, 851, 1477, 1726, 1759
HspAI GCGC 1 cut(s) 230
Kpn2I TCCGGA 1 cut(s) 313
Kzo9I GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
LmnI GCTCC 2 cut(s) 930, 1220
Lsp1109I GCAGC 7 cut(s) 498, 712, 715, 1086, 1210, 1291, 1870
LweI GCATC 4 cut(s) 427, 524, 1468, 2012
MaeI CTAG 7 cut(s) 71, 135, 303, 353, 590, 605, 714
MaeII ACGT 3 cut(s) 28, 844, 892
MaeIII GTNAC 2 cut(s) 1114, 1714
MalI GATC 9 cut(s) 41, 60, 255, 804, 1325, 1345, 1383, 1516, 1878
MboI GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
MfeI CAATTG 1 cut(s) 1188
MflI RGATCY 2 cut(s) 58, 1323
MhlI GDGCHC 4 cut(s) 142, 268, 428, 1609
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 8 cut(s) 43, 157, 224, 272, 631, 1127, 1144, 1188
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 1 cut(s) 1256
MmeI TCCRAC 1 cut(s) 1982
Mox20I TGGCCA 1 cut(s) 189
MroI TCCGGA 1 cut(s) 313
MscI TGGCCA 1 cut(s) 189
MseI TTAA 8 cut(s) 11, 114, 153, 528, 738, 1595, 1686, 1985
MslI CAYNNNNRTG 3 cut(s) 599, 732, 1371
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 2 cut(s) 233, 314
MspR9I CCNGG 4 cut(s) 53, 90, 768, 997
MunI CAATTG 1 cut(s) 1188
Mva1269I GAATGC 1 cut(s) 1886
MvaI CCWGG 4 cut(s) 53, 90, 768, 997
MwoI GCNNNNNNNGC 8 cut(s) 423, 492, 674, 683, 997, 1058, 1080, 1229
NdeII GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
NlaIII CATG 6 cut(s) 598, 731, 851, 1477, 1726, 1759
NlaIV GGNNCC 1 cut(s) 932
PctI GAATGC 1 cut(s) 1886
PfeI GAWTC 4 cut(s) 145, 950, 1821, 1990
PfoI TCCNGGA 1 cut(s) 51
PleI GAGTC 1 cut(s) 1256
PpsI GAGTC 1 cut(s) 1256
PshBI ATTAAT 1 cut(s) 1686
Psp6I CCWGG 4 cut(s) 51, 88, 766, 995
PspEI GGTNACC 1 cut(s) 1114
PspFI CCCAGC 2 cut(s) 176, 1481
PspGI CCWGG 4 cut(s) 51, 88, 766, 995
PspN4I GGNNCC 1 cut(s) 932
PspPI GGNCC 3 cut(s) 494, 608, 1393
PstI CTGCAG 2 cut(s) 702, 1735
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 2 cut(s) 58, 1323
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 7 cut(s) 324, 347, 523, 581, 891, 906, 1902
RsaNI GTAC 7 cut(s) 323, 346, 522, 580, 890, 905, 1901
RseI CAYNNNNRTG 3 cut(s) 599, 732, 1371
SaqAI TTAA 8 cut(s) 11, 114, 153, 528, 738, 1595, 1686, 1985
Sau3AI GATC 9 cut(s) 39, 58, 253, 802, 1323, 1343, 1381, 1514, 1876
Sau96I GGNCC 3 cut(s) 494, 608, 1393
ScaI AGTACT 2 cut(s) 523, 581
SchI GAGTC 1 cut(s) 1256
ScrFI CCNGG 4 cut(s) 53, 90, 768, 997
SduI GDGCHC 4 cut(s) 142, 268, 428, 1609
SfaNI GCATC 4 cut(s) 427, 524, 1468, 2012
SfcI CTRYAG 2 cut(s) 698, 1731
SinI GGWCC 1 cut(s) 1393
SmiMI CAYNNNNRTG 3 cut(s) 599, 732, 1371
SmlI CTYRAG 2 cut(s) 1106, 1929
SmoI CTYRAG 2 cut(s) 1106, 1929
SpeI ACTAGT 1 cut(s) 302
Sse9I AATT 8 cut(s) 43, 157, 224, 272, 631, 1127, 1144, 1188
SsiI CCGC 6 cut(s) 85, 414, 637, 1232, 1865, 1942
SspI AATATT 1 cut(s) 972
SspMI CTAG 7 cut(s) 71, 135, 303, 353, 590, 605, 714
StyD4I CCNGG 4 cut(s) 51, 88, 766, 995
StyI CCWWGG 3 cut(s) 190, 1300, 1624
TaaI ACNGT 5 cut(s) 112, 521, 889, 1101, 2026
TaiI ACGT 3 cut(s) 31, 847, 895
TaqI TCGA 3 cut(s) 25, 1791, 1949
TaqII GACCGA 1 cut(s) 1318
TasI AATT 8 cut(s) 43, 157, 224, 272, 631, 1127, 1144, 1188
TatI WGTACW 5 cut(s) 322, 345, 521, 579, 904
TauI GCSGC 3 cut(s) 88, 417, 1868
TfiI GAWTC 4 cut(s) 145, 950, 1821, 1990
Tru1I TTAA 8 cut(s) 11, 114, 153, 528, 738, 1595, 1686, 1985
Tru9I TTAA 8 cut(s) 11, 114, 153, 528, 738, 1595, 1686, 1985
TscAI CASTG 6 cut(s) 684, 1325, 1609, 1666, 1819, 1867
TseI GCWGC 7 cut(s) 486, 700, 703, 1074, 1223, 1279, 1883
TspDTI ATGAA 7 cut(s) 306, 1139, 1149, 1367, 1437, 1524, 1879
TspGWI ACGGA 2 cut(s) 1857, 1886
TspRI CASTG 6 cut(s) 684, 1325, 1609, 1666, 1819, 1867
VneI GTGCAC 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 1393
VspI ATTAAT 1 cut(s) 1686
XapI RAATTY 3 cut(s) 157, 631, 1127
XbaI TCTAGA 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 1317
XmiI GTMKAC 2 cut(s) 516, 763
XspI CTAG 7 cut(s) 71, 135, 303, 353, 590, 605, 714
ZraI GACGTC 1 cut(s) 845
ZrmI AGTACT 2 cut(s) 523, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.