Rorug02G0009900

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000629
Physical Location & Seq
Reverse (-)
817081 .. 890781
73701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0009900.1

Sequence Viewer

Length: 1578 bp
ATGGCTCACACTATCGCTCGCGCCACTCTCGGCCTCACTCAGATATCTCCGGCCTTTGAAGCCCCTAAGCTCACTTTGGCGACGAAAGAGGTTGATGTAACGGAATGGAAAGGGGACCTGCTTGCGGTTGGTGTTACAGAGAAGGACTTGGCGAAAGATCAGAACTCGAAATTCGAAAATCCGATTTTGAAGAAGCTGGATTCCTGTTTGGGTGGGGTGTTGGCTGAGGTCTCGACTGAGGAGGACTTCACAGGAAAGTCTGGCCAGTCTACGGTTGTTAGGCTTCCTGGTCTTGGCTCGAAGAGGGTTGGCTTGTTTGGGCTTGGACAATCTGCTTCGTCTACAGAAGCTTTTAAGGGTCTTGGGGAGGCTGCAGCAGCAGCGGCAAAGACTGCTCAAGCAAGTGATATTGCTATTGTGCTTGCTTCCTCGGAGGGGCTTTCTGCAAAATCTTGCACAGCTTCTGCAATAGCATCTGGAGCTGTTTTGGGGATATATGAAGACAACAGGTATAAATCTGAGTCAAAAAAACCAGCTCTCAAGTCTGTGGACATTCTTGGTCTTGGAACTGGACCTGAACTAGAGAAGAAGCTCAAATATGCTCAAGATGTTGCTGCTGGAATAATACTTGGGAAAGAGCTCGTGAATTCACCTGCCAATGTTCTCACACCTGGGAAACTAGCAGAAGAGGCCTCAAATATTGCTTCCTTATACAGTGATGTTCTTTCTGCAAAGATATTGAATGAAGAGCAATGCAAAGATCTGAAAATGGGTTCCTATTTGGCTGTGGCTGCAGCCTCAGCAAATCCTCCACATTTTATTCACTTGACCTACAAACCCCCTGGTGGACCTGCTAAGCTTAAATTAGCATTGGTGGGTAAAGGTCTGACTTTTGACAGTGGTGGCTACAACATCAAGGCAGGACCTGGCAGTATAATTGAGCTTATGAAGTTTGATATGGGAGGTTCAGCTGCTGTATTAGGTGCAGCAAAAGCCATTGGTCAAATCAAACCTTCTGGTGTAGAGGTTCATTTCATTGTCGCAGCTTGTGAGAATATGATTAGTGGAACAGGTATGAGGCCTGGAGACATTGTCACAGCTTCCAATGGAAAGACCATTGAGGTCAATAACACAGATGCTGAAGGCAGACTGACTTTGGCAGATGCATTGGTATATGCTTGTAATCAAGGTGTTGACAAGATTGTTGACCTTGCAAGCTTGACTGGTGCCTGCAGAGTCGCCCTTGGACCGTCTATTGCAGGTGTCTTCACACCCAGCGATGACCTCGCGAAGGAGGTATTTGCAGCTTCGGAGATAAGTGGGGAGAAATTTTGGAGAATGCCAATGGAGGAGAGTTATTGGGAATCAATGAAATCAGGAGTAGCTGATATGGTCAACACTGGTGATCGTCAAGGTGGTGCTATCAGTGCAGCTCTCTTCTTGAAACAGTTTGTTGATGAAAAGGTTCAGTGGATGCATATTGACATAGCTGGGCCTGTTTGGAGTGTTAAGAAACGCGCTGGGACAGGGTTTGCTATTTCTACCCTGGTGGAGTGGGTTTTGAAGAACTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

525

Amino Acids

54.62

Weight (kDa)

5.77

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M17_N PF02789 42 - 174 1.4e-26 Cytosol aminopeptidase family, N-terminal domain
Peptidase_M17 PF00883 211 - 517 2.4e-113 Cytosol aminopeptidase family, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 661, 1250
Acc36I ACCTGC 4 cut(s) 126, 661, 859, 1250
AccB1I GGYRCC 1 cut(s) 1226
AccI GTMKAC 2 cut(s) 269, 341
AccII CGCG 3 cut(s) 21, 1289, 1518
AciI CCGC 2 cut(s) 125, 383
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 3 cut(s) 170, 646, 1328
AcuI CTGAAG 1 cut(s) 1161
AfiI CCNNNNNNNGG 6 cut(s) 124, 271, 293, 435, 845, 1291
AgsI TTSAA 5 cut(s) 59, 190, 742, 1444, 1564
AjnI CCWGG 6 cut(s) 286, 670, 841, 925, 1081, 1545
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 2 cut(s) 235, 1080
AlwNI CAGNNNCTG 4 cut(s) 464, 926, 974, 1139
AoxI GGCC 6 cut(s) 31, 51, 262, 690, 1079, 1493
ApoI RAATTY 3 cut(s) 170, 646, 1328
Asp700I GAANNNNTTC 1 cut(s) 1464
AspLEI GCGC 2 cut(s) 23, 1520
AspS9I GGNCC 6 cut(s) 115, 572, 848, 923, 1247, 1493
AsuHPI GGTGA 2 cut(s) 642, 1415
AsuII TTCGAA 1 cut(s) 174
AvaII GGWCC 5 cut(s) 115, 572, 848, 923, 1247
BalI TGGCCA 1 cut(s) 264
BanI GGYRCC 1 cut(s) 1226
BanII GRGCYC 1 cut(s) 642
BauI CACGAG 1 cut(s) 641
BbsI GAAGAC 2 cut(s) 507, 1258
Bbv12I GWGCWC 1 cut(s) 642
BbvCI CCTCAGC 2 cut(s) 225, 799
BciT130I CCWGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
BcoDI GTCTC 2 cut(s) 235, 1080
BfaI CTAG 2 cut(s) 581, 680
BfmI CTRYAG 4 cut(s) 342, 372, 792, 1231
BfuAI ACCTGC 4 cut(s) 126, 661, 859, 1250
BglII AGATCT 1 cut(s) 760
BlpI GCTNAGC 1 cut(s) 855
Bme1390I CCNGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
Bme18I GGWCC 5 cut(s) 115, 572, 848, 923, 1247
BmgT120I GGNCC 6 cut(s) 115, 572, 848, 923, 1247, 1493
BmiI GGNNCC 3 cut(s) 116, 775, 1228
BmrFI CCNGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
BmsI GCATC 4 cut(s) 482, 1126, 1153, 1464
BpiI GAAGAC 2 cut(s) 507, 1258
BpmI CTGGAG 2 cut(s) 498, 1104
Bpu10I CCTNAGC 3 cut(s) 66, 225, 799
Bpu1102I GCTNAGC 1 cut(s) 855
Bpu14I TTCGAA 1 cut(s) 174
BpuEI CTTGAG 3 cut(s) 381, 524, 588
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 5 cut(s) 429, 671, 841, 1243, 1545
BsaXI ACNNNNNCTCC 2 cut(s) 1077, 1107
Bsc4I CCNNNNNNNGG 6 cut(s) 124, 271, 293, 435, 845, 1291
Bse1I ACTGG 4 cut(s) 265, 574, 1228, 1405
Bse3DI GCAATG 1 cut(s) 758
BseBI CCWGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
BseDI CCNNGG 5 cut(s) 429, 671, 841, 1243, 1545
BseGI GGATG 1 cut(s) 1479
BseLI CCNNNNNNNGG 6 cut(s) 124, 271, 293, 435, 845, 1291
BseMI GCAATG 1 cut(s) 758
BseMII CTCAG 5 cut(s) 53, 216, 228, 510, 813
BseNI ACTGG 4 cut(s) 265, 574, 1228, 1405
BseRI GAGGAG 3 cut(s) 254, 1364, 1561
BseYI CCCAGC 3 cut(s) 1274, 1490, 1520
BsgI GTGCAG 2 cut(s) 1005, 1449
Bsh1236I CGCG 3 cut(s) 21, 1289, 1518
BshFI GGCC 6 cut(s) 33, 53, 264, 692, 1081, 1495
BshNI GGYRCC 1 cut(s) 1226
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 2 cut(s) 128, 1537
BslI CCNNNNNNNGG 6 cut(s) 124, 271, 293, 435, 845, 1291
BsmAI GTCTC 2 cut(s) 235, 1080
BsmFI GGGAC 2 cut(s) 128, 1537
BsmI GAATGC 1 cut(s) 1344
BsnI GGCC 6 cut(s) 33, 53, 264, 692, 1081, 1495
Bso31I GGTCTC 1 cut(s) 235
Bsp119I TTCGAA 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 3 cut(s) 157, 760, 1405
Bsp1720I GCTNAGC 1 cut(s) 855
Bsp68I TCGCGA 1 cut(s) 1289
BspACI CCGC 2 cut(s) 125, 383
BspANI GGCC 6 cut(s) 33, 53, 264, 692, 1081, 1495
BspCNI CTCAG 5 cut(s) 52, 217, 229, 511, 812
BspFNI CGCG 3 cut(s) 21, 1289, 1518
BspLI GGNNCC 3 cut(s) 116, 775, 1228
BspMAI CTGCAG 3 cut(s) 376, 796, 1235
BspMI ACCTGC 4 cut(s) 126, 661, 859, 1250
BspQI GCTCTTC 1 cut(s) 741
BspT104I TTCGAA 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 1226
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 758
BsrI ACTGG 4 cut(s) 265, 574, 1228, 1405
BssECI CCNNGG 5 cut(s) 429, 671, 841, 1243, 1545
BssMI GATC 3 cut(s) 157, 760, 1405
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 1243
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
Bst4CI ACNGT 5 cut(s) 274, 716, 899, 1251, 1449
Bst6I CTCTTC 4 cut(s) 296, 681, 741, 1442
BstAPI GCANNNNNTGC 1 cut(s) 392
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 5 cut(s) 19, 123, 423, 1216, 1231
BstDEI CTNAG 8 cut(s) 39, 66, 225, 237, 519, 799, 855, 1575
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 1 cut(s) 1479
BstFNI CGCG 3 cut(s) 21, 1289, 1518
BstHHI GCGC 2 cut(s) 23, 1520
BstKTI GATC 3 cut(s) 160, 763, 1408
BstMAI GTCTC 2 cut(s) 235, 1080
BstMBI GATC 3 cut(s) 157, 760, 1405
BstNI CCWGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
BstSCI CCNGG 6 cut(s) 286, 670, 841, 925, 1081, 1545
BstSFI CTRYAG 4 cut(s) 342, 372, 792, 1231
BstUI CGCG 3 cut(s) 21, 1289, 1518
BstV2I GAAGAC 2 cut(s) 507, 1258
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
BsuRI GGCC 6 cut(s) 33, 53, 264, 692, 1081, 1495
BtgZI GCGATG 1 cut(s) 1293
BtsCI GGATG 1 cut(s) 1479
BtsIMutI CAGTG 5 cut(s) 721, 904, 1398, 1432, 1475
BtuMI TCGCGA 1 cut(s) 1289
BveI ACCTGC 4 cut(s) 126, 661, 859, 1250
Cac8I GCNNGC 5 cut(s) 19, 123, 423, 1216, 1231
CaiI CAGNNNCTG 4 cut(s) 464, 926, 974, 1139
CfoI GCGC 2 cut(s) 23, 1520
Cfr13I GGNCC 6 cut(s) 115, 572, 848, 923, 1247, 1493
DdeI CTNAG 8 cut(s) 39, 66, 225, 237, 519, 799, 855, 1575
DpnI GATC 3 cut(s) 159, 762, 1407
DpnII GATC 3 cut(s) 157, 760, 1405
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 4 cut(s) 296, 681, 741, 1442
EarI CTCTTC 4 cut(s) 296, 681, 741, 1442
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 1243
Eco147I AGGCCT 2 cut(s) 692, 1081
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 235
Eco32I GATATC 1 cut(s) 45
Eco47I GGWCC 5 cut(s) 115, 572, 848, 923, 1247
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 1161
EcoICRI GAGCTC 1 cut(s) 640
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 2 cut(s) 115, 923
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 6 cut(s) 286, 670, 841, 925, 1081, 1545
EcoRV GATATC 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 1243
EcoT22I ATGCAT 2 cut(s) 1168, 1479
EcoT38I GRGCYC 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 1243
FaqI GGGAC 2 cut(s) 128, 1537
FblI GTMKAC 2 cut(s) 269, 341
FokI GGATG 1 cut(s) 1486
FriOI GRGCYC 1 cut(s) 642
FspBI CTAG 2 cut(s) 581, 680
GlaI GCGC 2 cut(s) 22, 1519
GsaI CCCAGC 3 cut(s) 1278, 1494, 1524
GsuI CTGGAG 2 cut(s) 498, 1104
HaeIII GGCC 6 cut(s) 33, 53, 264, 692, 1081, 1495
HapII CCGG 1 cut(s) 50
HhaI GCGC 2 cut(s) 23, 1520
Hin6I GCGC 2 cut(s) 21, 1518
HinP1I GCGC 2 cut(s) 21, 1518
HincII GTYRAC 3 cut(s) 1195, 1207, 1396
HindII GTYRAC 3 cut(s) 1195, 1207, 1396
HindIII AAGCTT 3 cut(s) 348, 857, 1216
HinfI GANTC 4 cut(s) 200, 521, 1236, 1364
HpaII CCGG 1 cut(s) 50
HphI GGTGA 2 cut(s) 642, 1415
Hpy166II GTNNAC 7 cut(s) 270, 342, 550, 848, 1195, 1207, 1396
Hpy188I TCNGA 8 cut(s) 42, 162, 183, 433, 520, 765, 888, 1312
Hpy188III TCNNGA 7 cut(s) 232, 477, 605, 643, 1288, 1377, 1441
Hpy8I GTNNAC 7 cut(s) 270, 342, 550, 848, 1195, 1207, 1396
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 136, 1023, 1136, 1285
HpyCH4III ACNGT 5 cut(s) 274, 716, 899, 1251, 1449
HpyF3I CTNAG 8 cut(s) 39, 66, 225, 237, 519, 799, 855, 1575
HspAI GCGC 2 cut(s) 21, 1518
Kzo9I GATC 3 cut(s) 157, 760, 1405
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 1 cut(s) 479
LweI GCATC 4 cut(s) 482, 1126, 1153, 1464
MaeI CTAG 2 cut(s) 581, 680
MaeIII GTNAC 3 cut(s) 97, 133, 1093
MalI GATC 3 cut(s) 159, 762, 1407
MboI GATC 3 cut(s) 157, 760, 1405
MboII GAAGA 9 cut(s) 202, 313, 512, 598, 698, 758, 1258, 1429, 1576
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 1 cut(s) 642
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 5 cut(s) 170, 646, 863, 936, 1328
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 530, 1245
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 2 cut(s) 1168, 1479
MroXI GAANNNNTTC 1 cut(s) 1464
MscI TGGCCA 1 cut(s) 264
MseI TTAA 3 cut(s) 354, 861, 1509
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 2 cut(s) 383, 971
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
Mva1269I GAATGC 1 cut(s) 1344
MvaI CCWGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
MvnI CGCG 3 cut(s) 21, 1289, 1518
NdeII GATC 3 cut(s) 157, 760, 1405
NlaIV GGNNCC 3 cut(s) 116, 775, 1228
NmeAIII GCCGAG 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 1093
NruI TCGCGA 1 cut(s) 1289
NsiI ATGCAT 2 cut(s) 1168, 1479
NspV TTCGAA 1 cut(s) 174
PaqCI CACCTGC 2 cut(s) 661, 1250
PceI AGGCCT 2 cut(s) 692, 1081
PciSI GCTCTTC 1 cut(s) 741
PctI GAATGC 1 cut(s) 1344
PdmI GAANNNNTTC 1 cut(s) 1464
PfeI GAWTC 2 cut(s) 200, 1364
PflFI GACNNNGTC 1 cut(s) 1091
PleI GAGTC 2 cut(s) 529, 1244
PpsI GAGTC 2 cut(s) 529, 1244
PpuMI RGGWCCY 2 cut(s) 115, 923
Psp124BI GAGCTC 1 cut(s) 642
Psp5II RGGWCCY 2 cut(s) 115, 923
Psp6I CCWGG 6 cut(s) 286, 670, 841, 925, 1081, 1545
PspFI CCCAGC 3 cut(s) 1274, 1490, 1520
PspGI CCWGG 6 cut(s) 286, 670, 841, 925, 1081, 1545
PspN4I GGNNCC 3 cut(s) 116, 775, 1228
PspPI GGNCC 6 cut(s) 115, 572, 848, 923, 1247, 1493
PspPPI RGGWCCY 2 cut(s) 115, 923
PstI CTGCAG 3 cut(s) 376, 796, 1235
PstNI CAGNNNCTG 4 cut(s) 464, 926, 974, 1139
PsuI RGATCY 1 cut(s) 760
PsyI GACNNNGTC 1 cut(s) 1091
PvuII CAGCTG 1 cut(s) 971
RruI TCGCGA 1 cut(s) 1289
SacI GAGCTC 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 3 cut(s) 354, 861, 1509
Sau3AI GATC 3 cut(s) 157, 760, 1405
Sau96I GGNCC 6 cut(s) 115, 572, 848, 923, 1247, 1493
SchI GAGTC 2 cut(s) 530, 1245
ScrFI CCNGG 6 cut(s) 288, 672, 843, 927, 1083, 1547
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 4 cut(s) 482, 1126, 1153, 1464
SfcI CTRYAG 4 cut(s) 342, 372, 792, 1231
SfuI TTCGAA 1 cut(s) 174
SinI GGWCC 5 cut(s) 115, 572, 848, 923, 1247
SmlI CTYRAG 3 cut(s) 396, 539, 603
SmoI CTYRAG 3 cut(s) 396, 539, 603
Sse9I AATT 5 cut(s) 170, 646, 863, 936, 1328
SseBI AGGCCT 2 cut(s) 692, 1081
SsiI CCGC 2 cut(s) 125, 383
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 581, 680
SstI GAGCTC 1 cut(s) 642
StuI AGGCCT 2 cut(s) 692, 1081
StyD4I CCNGG 6 cut(s) 286, 670, 841, 925, 1081, 1545
StyI CCWWGG 1 cut(s) 1243
TaaI ACNGT 5 cut(s) 274, 716, 899, 1251, 1449
TaqI TCGA 4 cut(s) 167, 174, 233, 299
TasI AATT 5 cut(s) 170, 646, 863, 936, 1328
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 2 cut(s) 200, 1364
Tru1I TTAA 3 cut(s) 354, 861, 1509
Tru9I TTAA 3 cut(s) 354, 861, 1509
TscAI CASTG 5 cut(s) 721, 904, 1405, 1432, 1475
TseFI GTSAC 1 cut(s) 1093
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 7 cut(s) 513, 759, 962, 1019, 1024, 1385, 1473
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 5 cut(s) 721, 904, 1405, 1432, 1475
Tth111I GACNNNGTC 1 cut(s) 1091
VpaK11BI GGWCC 5 cut(s) 115, 572, 848, 923, 1247
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 3 cut(s) 170, 646, 1328
XmiI GTMKAC 2 cut(s) 269, 341
XmnI GAANNNNTTC 1 cut(s) 1464
XspI CTAG 2 cut(s) 581, 680
Zsp2I ATGCAT 2 cut(s) 1168, 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.