MD15G1187100.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012557
Physical Location & Seq
Forward (+)
14778564 .. 14784384
5821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1187100.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGCCAGATTTAAGAAGTGGAGTTCGACGTTCAAAGAGGATCAATTATGTTCACGAAGATCCTGCTGTTCTAGTTCCAGCTGTGCGGCCTGGTGCTGGAAAAGTTCTGACTGGTAAAGGTAGGGGTAGAGGGCCTAGAGCCCCGAATCAAGGTAAGAATTCCAAGCTATTTGGTGCTGGAGTTGGTGGCCAAGGGCATAGAGGTTTGGGTTTTCCAGAGAGAGAATTAGTGCCGGTTCACGACGAATTGGTAGCTCAGAAAGGTGCACAGAAATTGGTTGCTATGGAGGATGAAGGAAGCACTAATGCACTTCCGGACAGAGTACAAGTTGGCAAATCTCCTATGTACAAGCTAGACAGAAAGTTGGGGAAAGGGGGTTTCGGACAAGTTTATGTGGGAAGAAGGATGACTGGCGGCATAGGGTGCTCTGGAGCTGATGCATATGAGGTTGCTCTGAAGCTTGAAAGTAAAAATGGTAAAGGTTGCAGTCACGGCCCTCCTTCTGAGTGGCAAGTATACAGTACTCTTAATGGATGCTATGGGCTTCCACTCGTCCATTACAAAGGTCAACAGGGAGAGTACTATATCCTAATCATGGATATGCTAGGCCCAAGTTTATGGGATGTTTGGAATTCTCGCAATCAGTTGCTGTCTGAAGAGATGGTTGCTTGTATAGCGGTGGAGGCAATATCGATTCTGCAGCAGCTTCACACTAGAGGTTTTGTGCATGGAGATGTTAAGCCAGAAAACTTTTTACTTGGTCTACCTGGAACATCTAATGAGAAGAAGTTATACCTTGTTGATCTTGGTTTGGCTTCCAGATGGAGAGATGGGTCTTCTGGACGTCATGTTGTTTATGACCAAAAACCTGATGTTTTCAGGGGAACTGTACGTTATGCAAGTGTACATGCTCATTTAGGCAGGACAGGGAGCCGAAGGGATGACCTTGAATCGTTAGCTTATACCCTAATATTTCTCCTTAGAGGAAAACTTCCCTGGCAAGGCTACAATCAGGGAGAGAACAAAGGATTTCTTGTTTGTAAGAAGAAGATGGCAACTTCTGCTGAGACACTATGCAGCTTATGCCCTCCCCCATTTCAACAGTTTCTTGAGATAGTGACCAATATGAAATTCGATGAAGAACCAAATTACTTAAAGCTTATTTCCCTATTTGATAATAGCATTGGTGCAATTGCCTCTCTGCGACCTATTAGAACTGATGGAGCTGCAAAGGCGGGTCAAAAAAGGGGAAGATTGCCTGTTGACTCGGTAGATGATGAGCAACCTAAGAAAAAAGTTCGCCAAGGTAGTCCAGCTACTCAGTGGATCTCGGTCTATAATACCAGATCATCAATGAAGCAAAGGTATCACTACAATGTTTTGGATCAAAGACTGGACCAGCACATTGATAAAGGAAAGGAAGATGGTTTGTATATTAGTTGCGTTGCTTCGTCTGCGAATATGTGGGCTATCGTCATGGATGCAGGGACTGGCTTTACATCCCAGGTTTATGAACGGTCATCTATTTTCTTGCACAAGGAATGGATAATGGAACAGTGGGACAAGAACTACTACATCACTTCACTTGCTGGTGCATTCAACGGCAGTGCCCTGGTGGTAATGTCCCAAGGTGTACCTTATACTCAACAATCCTACAAAGTCAGTGATGTGTTCCCTTTCAAATGGATTCATAAGAAGTGGAAAGAAGGTTTCTCTGTTACCTCCATGACTACTGCGGGTAGCAGATGGGGTATTGTTATGTCCCGTAATGCAGGTTATTCTAGTCAGGTTGTTGAACTTGATTTCCTATATCCCAGTGAAGGCATCCATAGGAGATGGGAGGATGGATATCGGATTACCTCAACTGCTGCTACAGAGGATCAAGCTGCATTCATACTTAGTGTACCCAAAAAGAAATCACCAGATATTGCTCAAGAAACTCTACGGACATCAATTTTTCCAACATACCAAGTGAAGGAAAAATGGTTGAAGAATCTCTATATTGCATCCATCAGCTATGGAAGAACAGTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007165 GO:0007166 GO:0007267 GO:0007346 GO:0008150 GO:0008152 GO:0008360 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010564 GO:0010604 GO:0015630 GO:0016055 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019222 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032879 GO:0032886 GO:0033043 GO:0035567 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051493 GO:0051716 GO:0051726 GO:0060069 GO:0060255 GO:0060341 GO:0061136 GO:0065007 GO:0065008 GO:0070507 GO:0071704 GO:0080090 GO:0098657 GO:0140096 GO:0198738 GO:1901564 GO:1901800 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1905114 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

679

Amino Acids

75.5

Weight (kDa)

9.39

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 116 - 342 2.3e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 117 - 277 4.6e-07 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 220 - 325 4.1e-07 Fungal protein kinase
DUF7477 PF24289 411 - 678 2.8e-150 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05050 FvH4_1g05050 FvH4_1g05050
malus_domestica MD02G1048800.v1.1 MD15G1187100.v1.1
prunus_persica Prupe.7G229400_v2.0.a1
pyrus_communis pycom02g04090 pycom15g16760
rosa_chinensis RchiOBHm_Chr2g0090661
rosa_laevigata RLG00000016134
rosa_multiflora Rmu_sc0016852.1_g000008
rosa_roxburghii Rroxscaffold_2G00150900
rosa_rugosa Rorug02G0009900 Rorug02G0009900
rosa_samantha Rh2AG055800 Rh2BG054200 Rh2CG056500 Rh2DG055100
rosa_wichuraiana Rw2G004700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 847
Acc36I ACCTGC 1 cut(s) 1763
AccI GTMKAC 2 cut(s) 516, 763
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 5 cut(s) 85, 414, 677, 1235, 1736
AclWI GGATC 5 cut(s) 47, 53, 1334, 1392, 1887
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 3 cut(s) 157, 631, 1130
AcuI CTGAAG 2 cut(s) 476, 675
AcyI GRCGYC 1 cut(s) 844
AfaI GTAC 8 cut(s) 324, 347, 523, 581, 891, 906, 1635, 1905
AfiI CCNNNNNNNGG 6 cut(s) 95, 149, 595, 1001, 1820, 1975
AgsI TTSAA 8 cut(s) 33, 464, 950, 1100, 1600, 1681, 1796, 1990
AjnI CCWGG 5 cut(s) 88, 766, 995, 1503, 1611
AjuI GAANNNNNNNTTGG 2 cut(s) 1115, 1147
AloI GAACNNNNNNTCC 2 cut(s) 1788, 1820
Alw21I GWGCWC 2 cut(s) 268, 428
Alw26I GTCTC 1 cut(s) 1061
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 5 cut(s) 47, 53, 1334, 1392, 1887
AlwNI CAGNNNCTG 2 cut(s) 649, 1490
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 5 cut(s) 86, 131, 187, 493, 607
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 6 cut(s) 700, 703, 1077, 1226, 1868, 1886
ApoI RAATTY 3 cut(s) 157, 631, 1130
AspS9I GGNCC 4 cut(s) 131, 494, 608, 1396
AsuHPI GGTGA 1 cut(s) 1911
AvaII GGWCC 1 cut(s) 1396
BaeGI GKGCMC 2 cut(s) 268, 1612
BalI TGGCCA 1 cut(s) 189
BanII GRGCYC 1 cut(s) 142
BarI GAAGNNNNNNTAC 2 cut(s) 776, 808
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 2 cut(s) 268, 428
BbvI GCAGC 6 cut(s) 712, 715, 1089, 1213, 1855, 1873
BceAI ACGGC 2 cut(s) 508, 1618
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciT130I CCWGG 5 cut(s) 90, 768, 997, 1505, 1613
BcoDI GTCTC 1 cut(s) 1061
BfaI CTAG 6 cut(s) 71, 135, 353, 605, 714, 1782
BfmI CTRYAG 2 cut(s) 698, 1872
BfuAI ACCTGC 1 cut(s) 1763
BisI GCNGC 8 cut(s) 86, 415, 701, 704, 1078, 1227, 1869, 1887
BlsI GCNGC 8 cut(s) 87, 416, 702, 705, 1079, 1228, 1870, 1888
BmcAI AGTACT 2 cut(s) 523, 581
Bme1390I CCNGG 5 cut(s) 90, 768, 997, 1505, 1613
Bme18I GGWCC 1 cut(s) 1396
BmgT120I GGNCC 4 cut(s) 131, 494, 608, 1396
BmiI GGNNCC 1 cut(s) 932
BmrFI CCNGG 5 cut(s) 90, 768, 997, 1505, 1613
BmrI ACTGGG 1 cut(s) 1809
BmsI GCATC 5 cut(s) 427, 524, 1471, 1833, 2015
BmuI ACTGGG 1 cut(s) 1809
BpiI GAAGAC 1 cut(s) 828
BpmI CTGGAG 2 cut(s) 198, 450
BpuEI CTTGAG 2 cut(s) 1130, 1917
Bsa29I ATCGAT 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 1848
BsaHI GRCGYC 1 cut(s) 844
BsaJI CCNNGG 6 cut(s) 190, 995, 1303, 1503, 1611, 1627
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 6 cut(s) 95, 149, 595, 1001, 1820, 1975
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 5 cut(s) 115, 415, 1398, 1495, 1815
Bse8I GATNNNNATC 1 cut(s) 1848
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 5 cut(s) 90, 768, 997, 1505, 1613
BseCI ATCGAT 1 cut(s) 692
BseDI CCNNGG 6 cut(s) 190, 995, 1303, 1503, 1611, 1627
BseJI GATNNNNATC 1 cut(s) 1848
BseLI CCNNNNNNNGG 6 cut(s) 95, 149, 595, 1001, 1820, 1975
BseMII CTCAG 4 cut(s) 269, 495, 1056, 1334
BseNI ACTGG 5 cut(s) 115, 415, 1398, 1495, 1815
BseSI GKGCMC 2 cut(s) 268, 1612
BseXI GCAGC 6 cut(s) 712, 715, 1089, 1213, 1855, 1873
BshFI GGCC 5 cut(s) 88, 133, 189, 495, 609
BshVI ATCGAT 1 cut(s) 692
BsiHKAI GWGCWC 2 cut(s) 268, 428
BsiSI CCGG 2 cut(s) 233, 314
BslFI GGGAC 4 cut(s) 1501, 1574, 1609, 1747
BslI CCNNNNNNNGG 6 cut(s) 95, 149, 595, 1001, 1820, 1975
BsmAI GTCTC 1 cut(s) 1061
BsmFI GGGAC 4 cut(s) 1501, 1574, 1609, 1747
BsmI GAATGC 2 cut(s) 1595, 1889
BsnI GGCC 5 cut(s) 88, 133, 189, 495, 609
Bsp1286I GDGCHC 4 cut(s) 142, 268, 428, 1612
Bsp13I TCCGGA 1 cut(s) 313
Bsp1407I TGTACA 2 cut(s) 345, 904
Bsp143I GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
BspACI CCGC 5 cut(s) 85, 414, 677, 1235, 1736
BspANI GGCC 5 cut(s) 88, 133, 189, 495, 609
BspCNI CTCAG 4 cut(s) 268, 496, 1057, 1333
BspDI ATCGAT 1 cut(s) 692
BspEI TCCGGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 932
BspMAI CTGCAG 1 cut(s) 702
BspMI ACCTGC 1 cut(s) 1763
BspPI GGATC 5 cut(s) 47, 53, 1334, 1392, 1887
BsrFI RCCGGY 1 cut(s) 232
BsrGI TGTACA 2 cut(s) 345, 904
BsrI ACTGG 5 cut(s) 115, 415, 1398, 1495, 1815
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 6 cut(s) 190, 995, 1303, 1503, 1611, 1627
BssMI GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
BssNAI GTATAC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 844
BssT1I CCWWGG 3 cut(s) 190, 1303, 1627
Bst1107I GTATAC 1 cut(s) 517
Bst2UI CCWGG 5 cut(s) 90, 768, 997, 1505, 1613
Bst4CI ACNGT 6 cut(s) 521, 889, 1104, 1518, 1557, 2029
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 1 cut(s) 844
BstAPI GCANNNNNTGC 3 cut(s) 423, 1061, 1083
BstAUI TGTACA 2 cut(s) 345, 904
BstDEI CTNAG 7 cut(s) 255, 504, 980, 1065, 1287, 1320, 1898
BstKTI GATC 7 cut(s) 42, 61, 805, 1329, 1349, 1387, 1882
BstMAI GTCTC 1 cut(s) 1061
BstMBI GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
BstMWI GCNNNNNNNGC 8 cut(s) 423, 492, 674, 683, 1061, 1083, 1232, 1454
BstNI CCWGG 5 cut(s) 90, 768, 997, 1505, 1613
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 5 cut(s) 88, 766, 995, 1503, 1611
BstSFI CTRYAG 2 cut(s) 698, 1872
BstSLI GKGCMC 2 cut(s) 268, 1612
BstV1I GCAGC 6 cut(s) 712, 715, 1089, 1213, 1855, 1873
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 2 cut(s) 58, 1326
BstXI CCANNNNNNTGG 1 cut(s) 618
BstYI RGATCY 2 cut(s) 58, 1326
BstZ17I GTATAC 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 692
BsuRI GGCC 5 cut(s) 88, 133, 189, 495, 609
BsuTUI ATCGAT 1 cut(s) 692
BtsI GCAGTG 1 cut(s) 1612
BtsIMutI CAGTG 6 cut(s) 1328, 1562, 1612, 1669, 1822, 2034
BveI ACCTGC 1 cut(s) 1763
CaiI CAGNNNCTG 2 cut(s) 649, 1490
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 4 cut(s) 131, 494, 608, 1396
ClaI ATCGAT 1 cut(s) 692
Csp6I GTAC 8 cut(s) 323, 346, 522, 580, 890, 905, 1634, 1904
CviAII CATG 6 cut(s) 595, 728, 848, 908, 1477, 1726
CviQI GTAC 8 cut(s) 323, 346, 522, 580, 890, 905, 1634, 1904
DdeI CTNAG 7 cut(s) 255, 504, 980, 1065, 1287, 1320, 1898
DpnI GATC 7 cut(s) 41, 60, 804, 1328, 1348, 1386, 1881
DpnII GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Eco130I CCWWGG 3 cut(s) 190, 1303, 1627
Eco24I GRGCYC 1 cut(s) 142
Eco32I GATATC 1 cut(s) 1850
Eco47I GGWCC 1 cut(s) 1396
Eco57I CTGAAG 2 cut(s) 476, 675
EcoO109I RGGNCCY 1 cut(s) 131
EcoRI GAATTC 2 cut(s) 157, 631
EcoRII CCWGG 5 cut(s) 88, 766, 995, 1503, 1611
EcoRV GATATC 1 cut(s) 1850
EcoT14I CCWWGG 3 cut(s) 190, 1303, 1627
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 3 cut(s) 190, 1303, 1627
FaeI CATG 6 cut(s) 598, 731, 851, 911, 1480, 1729
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 4 cut(s) 1501, 1574, 1609, 1747
FatI CATG 6 cut(s) 594, 727, 847, 907, 1476, 1725
FauI CCCGC 2 cut(s) 1228, 1729
FauNDI CATATG 1 cut(s) 442
FblI GTMKAC 2 cut(s) 516, 763
Fnu4HI GCNGC 8 cut(s) 86, 415, 701, 704, 1078, 1227, 1869, 1887
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 8 cut(s) 86, 415, 701, 704, 1078, 1227, 1869, 1887
FspBI CTAG 6 cut(s) 71, 135, 353, 605, 714, 1782
GluI GCNGC 8 cut(s) 86, 415, 701, 704, 1078, 1227, 1869, 1887
GsuI CTGGAG 2 cut(s) 198, 450
HaeIII GGCC 5 cut(s) 88, 133, 189, 495, 609
HapII CCGG 2 cut(s) 233, 314
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 6 cut(s) 598, 731, 851, 911, 1480, 1729
HincII GTYRAC 2 cut(s) 569, 1264
HindII GTYRAC 2 cut(s) 569, 1264
HindIII AAGCTT 2 cut(s) 458, 1157
HinfI GANTC 6 cut(s) 145, 694, 950, 1265, 1687, 1993
HpaII CCGG 2 cut(s) 233, 314
HphI GGTGA 1 cut(s) 1911
Hpy188I TCNGA 7 cut(s) 108, 258, 383, 456, 505, 655, 1854
Hpy99I CGWCG 2 cut(s) 30, 245
HpyAV CCTTC 7 cut(s) 287, 396, 510, 930, 1700, 1814, 1969
HpyCH4III ACNGT 6 cut(s) 521, 889, 1104, 1518, 1557, 2029
HpyCH4IV ACGT 3 cut(s) 28, 844, 892
HpyF10VI GCNNNNNNNGC 8 cut(s) 423, 492, 674, 683, 1061, 1083, 1232, 1454
HpyF3I CTNAG 7 cut(s) 255, 504, 980, 1065, 1287, 1320, 1898
HpySE526I ACGT 3 cut(s) 28, 844, 892
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 6 cut(s) 598, 731, 851, 911, 1480, 1729
Kpn2I TCCGGA 1 cut(s) 313
Kzo9I GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
LmnI GCTCC 3 cut(s) 431, 930, 1223
Lsp1109I GCAGC 6 cut(s) 712, 715, 1089, 1213, 1855, 1873
LweI GCATC 5 cut(s) 427, 524, 1471, 1833, 2015
MaeI CTAG 6 cut(s) 71, 135, 353, 605, 714, 1782
MaeII ACGT 3 cut(s) 28, 844, 892
MaeIII GTNAC 3 cut(s) 488, 1117, 1717
MalI GATC 7 cut(s) 41, 60, 804, 1328, 1348, 1386, 1881
MboI GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 2 cut(s) 58, 1326
MhlI GDGCHC 4 cut(s) 142, 268, 428, 1612
MlsI TGGCCA 1 cut(s) 189
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 1 cut(s) 1259
MmeI TCCRAC 1 cut(s) 1985
Mox20I TGGCCA 1 cut(s) 189
Mph1103I ATGCAT 1 cut(s) 442
MroI TCCGGA 1 cut(s) 313
MscI TGGCCA 1 cut(s) 189
MseI TTAA 4 cut(s) 11, 528, 738, 1154
MslI CAYNNNNRTG 3 cut(s) 599, 732, 1374
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 2 cut(s) 233, 314
MspR9I CCNGG 5 cut(s) 90, 768, 997, 1505, 1613
MunI CAATTG 1 cut(s) 1191
Mva1269I GAATGC 2 cut(s) 1595, 1889
MvaI CCWGG 5 cut(s) 90, 768, 997, 1505, 1613
MwoI GCNNNNNNNGC 8 cut(s) 423, 492, 674, 683, 1061, 1083, 1232, 1454
NdeI CATATG 1 cut(s) 442
NdeII GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
NlaIII CATG 6 cut(s) 598, 731, 851, 911, 1480, 1729
NlaIV GGNNCC 1 cut(s) 932
NmuCI GTSAC 2 cut(s) 488, 1117
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 911
PctI GAATGC 2 cut(s) 1595, 1889
PfeI GAWTC 5 cut(s) 145, 694, 950, 1687, 1993
PkrI GCNGC 8 cut(s) 87, 416, 702, 705, 1079, 1228, 1870, 1888
PleI GAGTC 1 cut(s) 1259
PpsI GAGTC 1 cut(s) 1259
Psp6I CCWGG 5 cut(s) 88, 766, 995, 1503, 1611
PspGI CCWGG 5 cut(s) 88, 766, 995, 1503, 1611
PspN4I GGNNCC 1 cut(s) 932
PspPI GGNCC 4 cut(s) 131, 494, 608, 1396
PstI CTGCAG 1 cut(s) 702
PstNI CAGNNNCTG 2 cut(s) 649, 1490
PsuI RGATCY 2 cut(s) 58, 1326
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 8 cut(s) 324, 347, 523, 581, 891, 906, 1635, 1905
RsaNI GTAC 8 cut(s) 323, 346, 522, 580, 890, 905, 1634, 1904
RseI CAYNNNNRTG 3 cut(s) 599, 732, 1374
SaqAI TTAA 4 cut(s) 11, 528, 738, 1154
SatI GCNGC 8 cut(s) 86, 415, 701, 704, 1078, 1227, 1869, 1887
Sau3AI GATC 7 cut(s) 39, 58, 802, 1326, 1346, 1384, 1879
Sau96I GGNCC 4 cut(s) 131, 494, 608, 1396
ScaI AGTACT 2 cut(s) 523, 581
SchI GAGTC 1 cut(s) 1259
ScrFI CCNGG 5 cut(s) 90, 768, 997, 1505, 1613
SduI GDGCHC 4 cut(s) 142, 268, 428, 1612
SfaNI GCATC 5 cut(s) 427, 524, 1471, 1833, 2015
SfcI CTRYAG 2 cut(s) 698, 1872
SinI GGWCC 1 cut(s) 1396
SmiMI CAYNNNNRTG 3 cut(s) 599, 732, 1374
SmlI CTYRAG 2 cut(s) 1109, 1932
SmoI CTYRAG 2 cut(s) 1109, 1932
SsiI CCGC 5 cut(s) 85, 414, 677, 1235, 1736
SspI AATATT 1 cut(s) 972
SspMI CTAG 6 cut(s) 71, 135, 353, 605, 714, 1782
StyD4I CCNGG 5 cut(s) 88, 766, 995, 1503, 1611
StyI CCWWGG 3 cut(s) 190, 1303, 1627
TaaI ACNGT 6 cut(s) 521, 889, 1104, 1518, 1557, 2029
TaiI ACGT 3 cut(s) 31, 847, 895
TaqI TCGA 3 cut(s) 25, 692, 1134
TaqII GACCGA 1 cut(s) 1321
TatI WGTACW 5 cut(s) 322, 345, 521, 579, 904
TauI GCSGC 2 cut(s) 88, 417
TfiI GAWTC 5 cut(s) 145, 694, 950, 1687, 1993
Tru1I TTAA 4 cut(s) 11, 528, 738, 1154
Tru9I TTAA 4 cut(s) 11, 528, 738, 1154
TscAI CASTG 6 cut(s) 1328, 1562, 1612, 1669, 1822, 2034
TseFI GTSAC 2 cut(s) 488, 1117
TseI GCWGC 6 cut(s) 700, 703, 1077, 1226, 1868, 1886
Tsp45I GTSAC 2 cut(s) 488, 1117
TspDTI ATGAA 7 cut(s) 306, 1142, 1152, 1370, 1527, 1679, 1882
TspGWI ACGGA 1 cut(s) 1960
TspRI CASTG 6 cut(s) 1328, 1562, 1612, 1669, 1822, 2034
VneI GTGCAC 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 1396
XapI RAATTY 3 cut(s) 157, 631, 1130
XceI RCATGY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 1320
XmiI GTMKAC 2 cut(s) 516, 763
XspI CTAG 6 cut(s) 71, 135, 353, 605, 714, 1782
ZraI GACGTC 1 cut(s) 845
ZrmI AGTACT 2 cut(s) 523, 581
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.