Rroxscaffold_2G00150900

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012557
Physical Location & Seq
Reverse (-)
88259534 .. 88265969
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150900.1

Sequence Viewer

Length: 2028 bp
ATGCCAGATTTAAGAAGTGGAGTTCGGCGATCAAAGAGGATCAACAATGTCCAGGAAAATCCTGCTGTTTTAGTTCCAGCTGCACGGACTGGTGCAGGAAAAGGGTTGACTGGTAGAGGTAGGGGTAGAGGGTCTAGAGCCATGAATCAAGGGAAGAATTCCAAGCTGTTTGGTGCTGGAGTTGGTGGCCAAGGGAATGCAGGTTTGGATTTACCAAAAAGAGATTTGGTGGCGGTTCACGATGAATTGGTAGATCAGAAAGGTGCACAAAAATTGACTGCTATTGAGGATGAAGGGAGCAGTAGTCCACTTCCCGAAAGAGTACAGATTGGAAACTCCCCCATGTACAAGTTGGATAGAAAGCTGGGGAAAGGGGGCTTTGGACAAGTTTATGTAGGAAGAAGGGTGACAGGAGGATATGGGTGCTCTGGGCCTGAGGCCTTTGAGGTTGCGTTAAAGCTTGAGGGTCGAAACAGTAAAGGGTGCAGTCATGGCCCTCCTTATGAGTGGCAAGTATACAGCTCCCTTAATGGATGTTATGGAGTTCCCCACGTTCATTACAAAGGTCAACAGGGAGAATACTATATCCTGGTCATGGATATGCTGGGCCCCAGTTTATGGGATGTTTGGAATCGCAATCAGTTGCTGTCTGAAGAGATGGTTGCTTGTATAGCGGTGGAGGCAATCTCAATTCTAGAGCAGCTTCATATCAAAGGTTTTGTGCATGGAGACGTTAAGCCAGAAAACTTTTTGCTTGGTCAACCTGGAACAGCCAATGATAAGAAGTTATATCTTGTTGACCTTGGTTTGGCTTCAAGATGGAAGGAAACATCTGGACGTCATGTTGATTATGATCAAAAACCTGATGTTTTCAGGGGAACTGTTCGTTATGCAAGTGTACATGCTCATTTAGGCAGGACAGGGAGCCGAAGGGATGACCTTGAGTCATTAGCTTATACCCTTATATTTCTCCTTAGAGGCAAACTTCCCTGGCAAGGTTACAATGGGGAAAACAAAGGATTTCTTGTTTGCAAGAAGAAGATGGCAACTTCTCCTGAGATGCTATGTGGCTCATGTCCTCCTCCTTTTCAACAGTTTCTTGAGTTGGTGACCAATATGAAATTCGATGAAGAACCAAATTACTTAAAACTAATTTCTCTGTTTGATAACAACATTAATGTGAATGCTGCTCTTCGACCTATAAGAACTGATGGAGCTGCAAAGGTGGGCCAAAAAAGGGGAAGGTTGTTTCTTGAATCAGAAGATAGTGAGCAACCCAGGAAAAAAGTTAGACTAGGTTGCCCAGCCAATCAGTGGATCTCAGTCTATAATACCAGACCGTCGATGAAGCAAAGGTATCATTACAATGTTATGGACGTGAGAGTGGACCAGCACATTGACAAAGGGAAGGAAGATGGTTTGTATATTAGCTGTGTCGCTTCATCTGCGAATATGTGGGCTATAGTCATGGATGCAGGGACAGGTTTCACATCCCAAGTTTATGAATTATCTCCTGTTTTCTTGCACAAGGAGTGGATAATGGAACAGTGGGACAAGAACTACTATATCACTTCACTTGCTGGCGCAGGCAATGGCAGTGCCCTGGTGGTAATGTCCCAAGGCGTACCTTATACTCAGCAGTCGTACAAAGTCAGTGACGTATTCCCTTTTAAATGGATTAATAAGAAATGGAAAGAAGGTTTCTCTGTTACCTCCATGACTACTGCAGGTAGCAGATGGGGCATTGTCATGTCCAGAAATGCAGGTTATTCTAATCAGGTTGTTGAGATTGATTTCCTATATCCGAGCGAAGGCATCCATAGAAGATGGGAGAATGGATATCGTATTACCTCAACTGCTGCGACAGAGGATCAAGCTGCATTCATACTTAGTTCACCCAAGAAGAAATCACAAGATGTGGCTCAGGAAACTCTACGGACATCTGTTTTTCCAACTACGCATGTCAAGGAAAAATGGTTGAAGAATCTATATATTGCATCCATCTGCTATGGGAGAACAGTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007165 GO:0007166 GO:0007267 GO:0007346 GO:0008150 GO:0008152 GO:0008360 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010564 GO:0010604 GO:0015630 GO:0016055 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019222 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032879 GO:0032886 GO:0033043 GO:0035567 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051493 GO:0051716 GO:0051726 GO:0060069 GO:0060255 GO:0060341 GO:0061136 GO:0065007 GO:0065008 GO:0070507 GO:0071704 GO:0080090 GO:0098657 GO:0140096 GO:0198738 GO:1901564 GO:1901800 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1905114 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

675

Amino Acids

75.22

Weight (kDa)

9.26

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 116 - 346 3.2e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 117 - 344 4.8e-06 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 196 - 323 1.5e-08 Fungal protein kinase
DUF7477 PF24289 408 - 675 2.7e-154 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g05050 FvH4_1g05050 FvH4_1g05050
malus_domestica MD02G1048800.v1.1 MD15G1187100.v1.1
prunus_persica Prupe.7G229400_v2.0.a1
pyrus_communis pycom02g04090 pycom15g16760
rosa_chinensis RchiOBHm_Chr2g0090661
rosa_laevigata RLG00000016134
rosa_multiflora Rmu_sc0016852.1_g000008
rosa_roxburghii Rroxscaffold_2G00150900
rosa_rugosa Rorug02G0009900 Rorug02G0009900
rosa_samantha Rh2AG055800 Rh2BG054200 Rh2CG056500 Rh2DG055100
rosa_wichuraiana Rw2G004700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 841
Acc36I ACCTGC 3 cut(s) 191, 1718, 1754
AccB7I CCANNNNNTGG 2 cut(s) 618, 1314
AccI GTMKAC 1 cut(s) 516
AciI CCGC 2 cut(s) 233, 674
AclWI GGATC 3 cut(s) 47, 1325, 1878
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 2 cut(s) 157, 1121
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 838
AfaI GTAC 5 cut(s) 324, 347, 900, 1626, 1646
AfiI CCNNNNNNNGG 7 cut(s) 595, 618, 808, 995, 1237, 1314, 1811
AgsI TTSAA 4 cut(s) 816, 1091, 1256, 1981
AhdI GACNNNNNGTC 1 cut(s) 943
AjiI CACGTC 1 cut(s) 1378
AjnI CCWGG 6 cut(s) 51, 588, 763, 989, 1277, 1602
AjuI GAANNNNNNNTTGG 4 cut(s) 188, 220, 1106, 1138
Alw21I GWGCWC 2 cut(s) 268, 428
Alw26I GTCTC 1 cut(s) 723
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 3 cut(s) 47, 1325, 1878
AlwNI CAGNNNCTG 1 cut(s) 646
AoxI GGCC 6 cut(s) 187, 431, 438, 493, 607, 1228
ApaI GGGCCC 1 cut(s) 611
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 6 cut(s) 80, 700, 1187, 1217, 1859, 1877
ApoI RAATTY 2 cut(s) 157, 1121
AseI ATTAAT 2 cut(s) 1176, 1680
AspLEI GCGC 1 cut(s) 1586
AspS9I GGNCC 6 cut(s) 431, 494, 607, 608, 1228, 1387
AsuHPI GGTGA 3 cut(s) 418, 1120, 1887
AvaII GGWCC 1 cut(s) 1387
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 3 cut(s) 268, 611, 1603
BalI TGGCCA 1 cut(s) 189
BanII GRGCYC 1 cut(s) 611
Bbv12I GWGCWC 2 cut(s) 268, 428
BbvI GCAGC 6 cut(s) 67, 712, 1174, 1204, 1846, 1864
BccI CCATC 8 cut(s) 652, 813, 1036, 1205, 1409, 1731, 1821, 2009
BciT130I CCWGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
BclI TGATCA 1 cut(s) 853
BcoDI GTCTC 1 cut(s) 723
BfaI CTAG 3 cut(s) 135, 695, 1295
BfmI CTRYAG 2 cut(s) 1461, 1725
BfuAI ACCTGC 3 cut(s) 191, 1718, 1754
BisI GCNGC 6 cut(s) 81, 701, 1188, 1218, 1860, 1878
BlsI GCNGC 6 cut(s) 82, 702, 1189, 1219, 1861, 1879
Bme1390I CCNGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
Bme18I GGWCC 1 cut(s) 1387
BmeRI GACNNNNNGTC 1 cut(s) 943
BmgBI CACGTC 1 cut(s) 1378
BmgT120I GGNCC 6 cut(s) 431, 494, 607, 608, 1228, 1387
BmiI GGNNCC 3 cut(s) 609, 610, 926
BmrFI CCNGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 4 cut(s) 1050, 1462, 1824, 2006
BmuI ACTGGG 1 cut(s) 606
BplI GAGNNNNNCTC 2 cut(s) 671, 703
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 1923
BpuEI CTTGAG 3 cut(s) 482, 962, 1121
BsaBI GATNNNNATC 1 cut(s) 852
BsaHI GRCGYC 1 cut(s) 838
BsaJI CCNNGG 6 cut(s) 190, 802, 989, 1277, 1602, 1618
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 7 cut(s) 595, 618, 808, 995, 1237, 1314, 1811
Bse1I ACTGG 3 cut(s) 94, 115, 612
Bse21I CCTNAGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 1597
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
BseDI CCNNGG 6 cut(s) 190, 802, 989, 1277, 1602, 1618
BseGI GGATG 8 cut(s) 295, 539, 628, 940, 1477, 1490, 1815, 1997
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 7 cut(s) 595, 618, 808, 995, 1237, 1314, 1811
BseMI GCAATG 1 cut(s) 1597
BseMII CTCAG 5 cut(s) 426, 1047, 1335, 1649, 1937
BseNI ACTGG 3 cut(s) 94, 115, 612
BseRI GAGGAG 1 cut(s) 1071
BseSI GKGCMC 3 cut(s) 268, 611, 1603
BseXI GCAGC 6 cut(s) 67, 712, 1174, 1204, 1846, 1864
BseYI CCCAGC 3 cut(s) 364, 604, 1303
BsgI GTGCAG 3 cut(s) 66, 114, 505
BshFI GGCC 6 cut(s) 189, 433, 440, 495, 609, 1230
BsiHKAI GWGCWC 2 cut(s) 268, 428
BslFI GGGAC 3 cut(s) 1492, 1565, 1600
BslI CCNNNNNNNGG 7 cut(s) 595, 618, 808, 995, 1237, 1314, 1811
BsmAI GTCTC 1 cut(s) 723
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 3 cut(s) 1492, 1565, 1600
BsmI GAATGC 3 cut(s) 202, 1189, 1880
BsnI GGCC 6 cut(s) 189, 433, 440, 495, 609, 1230
Bsp120I GGGCCC 1 cut(s) 607
Bsp1286I GDGCHC 4 cut(s) 268, 428, 611, 1603
Bsp1407I TGTACA 2 cut(s) 345, 898
Bsp143I GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
BspACI CCGC 2 cut(s) 233, 674
BspANI GGCC 6 cut(s) 189, 433, 440, 495, 609, 1230
BspCNI CTCAG 5 cut(s) 427, 1048, 1334, 1648, 1936
BspHI TCATGA 1 cut(s) 2024
BspLI GGNNCC 3 cut(s) 609, 610, 926
BspMAI CTGCAG 1 cut(s) 1729
BspMI ACCTGC 3 cut(s) 191, 1718, 1754
BspPI GGATC 3 cut(s) 47, 1325, 1878
BspQI GCTCTTC 1 cut(s) 1197
BsrDI GCAATG 1 cut(s) 1597
BsrGI TGTACA 2 cut(s) 345, 898
BsrI ACTGG 3 cut(s) 94, 115, 612
BssECI CCNNGG 6 cut(s) 190, 802, 989, 1277, 1602, 1618
BssMI GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
BssNAI GTATAC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 838
BssT1I CCWWGG 3 cut(s) 190, 802, 1618
Bst1107I GTATAC 1 cut(s) 517
Bst2UI CCWGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
Bst4CI ACNGT 6 cut(s) 476, 883, 1095, 1341, 1548, 2020
Bst6I CTCTTC 2 cut(s) 648, 1197
BstACI GRCGYC 1 cut(s) 838
BstAUI TGTACA 2 cut(s) 345, 898
BstC8I GCNNGC 2 cut(s) 1582, 1588
BstDEI CTNAG 7 cut(s) 435, 974, 1056, 1321, 1635, 1889, 1923
BstEII GGTNACC 1 cut(s) 1108
BstF5I GGATG 8 cut(s) 295, 539, 628, 940, 1477, 1490, 1815, 1997
BstHHI GCGC 1 cut(s) 1586
BstKTI GATC 6 cut(s) 32, 42, 256, 856, 1320, 1873
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
BstMWI GCNNNNNNNGC 5 cut(s) 492, 671, 680, 1445, 1740
BstNI CCWGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
BstNSI RCATGY 2 cut(s) 905, 1964
BstPI GGTNACC 1 cut(s) 1108
BstSCI CCNGG 6 cut(s) 51, 588, 763, 989, 1277, 1602
BstSFI CTRYAG 2 cut(s) 1461, 1725
BstSLI GKGCMC 3 cut(s) 268, 611, 1603
BstV1I GCAGC 6 cut(s) 67, 712, 1174, 1204, 1846, 1864
BstX2I RGATCY 1 cut(s) 1317
BstYI RGATCY 1 cut(s) 1317
BstZ17I GTATAC 1 cut(s) 517
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 6 cut(s) 189, 433, 440, 495, 609, 1230
BtrI CACGTC 1 cut(s) 1378
BtsCI GGATG 8 cut(s) 295, 539, 628, 940, 1477, 1490, 1815, 1997
BtsI GCAGTG 1 cut(s) 1603
BtsIMutI CAGTG 4 cut(s) 1319, 1553, 1603, 1660
BveI ACCTGC 3 cut(s) 191, 1718, 1754
Cac8I GCNNGC 2 cut(s) 1582, 1588
CaiI CAGNNNCTG 1 cut(s) 646
CciI TCATGA 1 cut(s) 2024
CfoI GCGC 1 cut(s) 1586
Cfr13I GGNCC 6 cut(s) 431, 494, 607, 608, 1228, 1387
Csp6I GTAC 5 cut(s) 323, 346, 899, 1625, 1645
CviQI GTAC 5 cut(s) 323, 346, 899, 1625, 1645
DdeI CTNAG 7 cut(s) 435, 974, 1056, 1321, 1635, 1889, 1923
DpnI GATC 6 cut(s) 31, 41, 255, 855, 1319, 1872
DpnII GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
DraI TTTAAA 1 cut(s) 1672
DriI GACNNNNNGTC 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 648, 1197
Eam1105I GACNNNNNGTC 1 cut(s) 943
EarI CTCTTC 2 cut(s) 648, 1197
Eco130I CCWWGG 3 cut(s) 190, 802, 1618
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 611
Eco32I GATATC 1 cut(s) 1841
Eco47I GGWCC 1 cut(s) 1387
Eco57I CTGAAG 1 cut(s) 672
Eco81I CCTNAGG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 1108
EcoO109I RGGNCCY 1 cut(s) 608
EcoO65I GGTNACC 1 cut(s) 1108
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 6 cut(s) 51, 588, 763, 989, 1277, 1602
EcoRV GATATC 1 cut(s) 1841
EcoT14I CCWWGG 3 cut(s) 190, 802, 1618
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 3 cut(s) 190, 802, 1618
Esp3I CGTCTC 1 cut(s) 723
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FaqI GGGAC 3 cut(s) 1492, 1565, 1600
FbaI TGATCA 1 cut(s) 853
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 6 cut(s) 81, 701, 1188, 1218, 1860, 1878
FokI GGATG 8 cut(s) 302, 546, 635, 947, 1477, 1484, 1802, 1984
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 6 cut(s) 81, 701, 1188, 1218, 1860, 1878
FspBI CTAG 3 cut(s) 135, 695, 1295
GlaI GCGC 1 cut(s) 1585
GluI GCNGC 6 cut(s) 81, 701, 1188, 1218, 1860, 1878
GsaI CCCAGC 3 cut(s) 368, 608, 1307
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 6 cut(s) 189, 433, 440, 495, 609, 1230
HhaI GCGC 1 cut(s) 1586
Hin1I GRCGYC 1 cut(s) 838
Hin6I GCGC 1 cut(s) 1584
HinP1I GCGC 1 cut(s) 1584
HincII GTYRAC 4 cut(s) 108, 569, 761, 799
HindII GTYRAC 4 cut(s) 108, 569, 761, 799
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 5 cut(s) 145, 631, 944, 1256, 1984
HphI GGTGA 3 cut(s) 418, 1120, 1887
Hpy188I TCNGA 4 cut(s) 258, 652, 1261, 1806
Hpy99I CGWCG 1 cut(s) 1345
HpyAV CCTTC 8 cut(s) 287, 396, 817, 924, 1236, 1402, 1691, 1805
HpyCH4III ACNGT 6 cut(s) 476, 883, 1095, 1341, 1548, 2020
HpyCH4IV ACGT 5 cut(s) 552, 732, 838, 1377, 1659
HpyF10VI GCNNNNNNNGC 5 cut(s) 492, 671, 680, 1445, 1740
HpyF3I CTNAG 7 cut(s) 435, 974, 1056, 1321, 1635, 1889, 1923
HpySE526I ACGT 5 cut(s) 552, 732, 838, 1377, 1659
Hsp92I GRCGYC 1 cut(s) 838
HspAI GCGC 1 cut(s) 1584
Ksp22I TGATCA 1 cut(s) 853
Kzo9I GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
LguI GCTCTTC 1 cut(s) 1197
LmnI GCTCC 4 cut(s) 297, 527, 924, 1214
Lsp1109I GCAGC 6 cut(s) 67, 712, 1174, 1204, 1846, 1864
LweI GCATC 4 cut(s) 1050, 1462, 1824, 2006
MaeI CTAG 3 cut(s) 135, 695, 1295
MaeII ACGT 5 cut(s) 552, 732, 838, 1377, 1659
MaeIII GTNAC 5 cut(s) 406, 998, 1108, 1655, 1708
MalI GATC 6 cut(s) 31, 41, 255, 855, 1319, 1872
MboI GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
MflI RGATCY 1 cut(s) 1317
MhlI GDGCHC 4 cut(s) 268, 428, 611, 1603
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 8 cut(s) 157, 245, 272, 690, 1121, 1138, 1152, 1505
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 1 cut(s) 953
MmeI TCCRAC 2 cut(s) 333, 1976
Mox20I TGGCCA 1 cut(s) 189
MscI TGGCCA 1 cut(s) 189
MseI TTAA 8 cut(s) 11, 455, 528, 735, 1145, 1176, 1671, 1680
MslI CAYNNNNRTG 4 cut(s) 599, 1178, 1365, 1748
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 80
MspR9I CCNGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
Mva1269I GAATGC 3 cut(s) 202, 1189, 1880
MvaI CCWGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
MwoI GCNNNNNNNGC 5 cut(s) 492, 671, 680, 1445, 1740
NdeII GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
NlaIV GGNNCC 3 cut(s) 609, 610, 926
NmuCI GTSAC 3 cut(s) 406, 1108, 1655
NspI RCATGY 2 cut(s) 905, 1964
PagI TCATGA 1 cut(s) 2024
PceI AGGCCT 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 1197
PctI GAATGC 3 cut(s) 202, 1189, 1880
PfeI GAWTC 4 cut(s) 145, 631, 1256, 1984
PflMI CCANNNNNTGG 2 cut(s) 618, 1314
PfoI TCCNGGA 1 cut(s) 51
PkrI GCNGC 6 cut(s) 82, 702, 1189, 1219, 1861, 1879
PleI GAGTC 1 cut(s) 952
PpsI GAGTC 1 cut(s) 952
PshBI ATTAAT 2 cut(s) 1176, 1680
Psp6I CCWGG 6 cut(s) 51, 588, 763, 989, 1277, 1602
PspEI GGTNACC 1 cut(s) 1108
PspFI CCCAGC 3 cut(s) 364, 604, 1303
PspGI CCWGG 6 cut(s) 51, 588, 763, 989, 1277, 1602
PspN4I GGNNCC 3 cut(s) 609, 610, 926
PspOMI GGGCCC 1 cut(s) 607
PspPI GGNCC 6 cut(s) 431, 494, 607, 608, 1228, 1387
PstI CTGCAG 1 cut(s) 1729
PstNI CAGNNNCTG 1 cut(s) 646
PsuI RGATCY 1 cut(s) 1317
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 5 cut(s) 324, 347, 900, 1626, 1646
RsaNI GTAC 5 cut(s) 323, 346, 899, 1625, 1645
RseI CAYNNNNRTG 4 cut(s) 599, 1178, 1365, 1748
SapI GCTCTTC 1 cut(s) 1197
SaqAI TTAA 8 cut(s) 11, 455, 528, 735, 1145, 1176, 1671, 1680
SatI GCNGC 6 cut(s) 81, 701, 1188, 1218, 1860, 1878
Sau3AI GATC 6 cut(s) 29, 39, 253, 853, 1317, 1870
Sau96I GGNCC 6 cut(s) 431, 494, 607, 608, 1228, 1387
SchI GAGTC 1 cut(s) 953
ScrFI CCNGG 6 cut(s) 53, 590, 765, 991, 1279, 1604
SduI GDGCHC 4 cut(s) 268, 428, 611, 1603
SfaNI GCATC 4 cut(s) 1050, 1462, 1824, 2006
SfcI CTRYAG 2 cut(s) 1461, 1725
SinI GGWCC 1 cut(s) 1387
SmiMI CAYNNNNRTG 4 cut(s) 599, 1178, 1365, 1748
SmlI CTYRAG 3 cut(s) 461, 941, 1100
SmoI CTYRAG 3 cut(s) 461, 941, 1100
Sse9I AATT 8 cut(s) 157, 245, 272, 690, 1121, 1138, 1152, 1505
SseBI AGGCCT 1 cut(s) 440
SsiI CCGC 2 cut(s) 233, 674
SspMI CTAG 3 cut(s) 135, 695, 1295
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 6 cut(s) 51, 588, 763, 989, 1277, 1602
StyI CCWWGG 3 cut(s) 190, 802, 1618
TaaI ACNGT 6 cut(s) 476, 883, 1095, 1341, 1548, 2020
TaiI ACGT 5 cut(s) 555, 735, 841, 1380, 1662
TaqI TCGA 4 cut(s) 469, 1125, 1195, 1343
TasI AATT 8 cut(s) 157, 245, 272, 690, 1121, 1138, 1152, 1505
TatI WGTACW 3 cut(s) 322, 345, 898
TfiI GAWTC 4 cut(s) 145, 631, 1256, 1984
Tru1I TTAA 8 cut(s) 11, 455, 528, 735, 1145, 1176, 1671, 1680
Tru9I TTAA 8 cut(s) 11, 455, 528, 735, 1145, 1176, 1671, 1680
TscAI CASTG 4 cut(s) 1319, 1553, 1603, 1660
TseFI GTSAC 3 cut(s) 406, 1108, 1655
TseI GCWGC 6 cut(s) 80, 700, 1187, 1217, 1859, 1877
Tsp45I GTSAC 3 cut(s) 406, 1108, 1655
TspGWI ACGGA 2 cut(s) 100, 1951
TspRI CASTG 4 cut(s) 1319, 1553, 1603, 1660
Van91I CCANNNNNTGG 2 cut(s) 618, 1314
VneI GTGCAC 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 1387
VspI ATTAAT 2 cut(s) 1176, 1680
XapI RAATTY 2 cut(s) 157, 1121
XbaI TCTAGA 2 cut(s) 134, 694
XceI RCATGY 2 cut(s) 905, 1964
XcmI CCANNNNNNNNNTGG 2 cut(s) 349, 1311
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 3 cut(s) 135, 695, 1295
ZraI GACGTC 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.