Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0012373

Physical Location & Seq

Genomic Coordinates Forward (+)

4558745 .. 4562969

Gene Structure 4225 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1056300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGCAAATTCTTCACGTTCCTTCTTCGCTATATCTCTCATTTCAAAACGTAAAATCTCATGAAGATGCAGGATTAATATCTTCGTATGAGAAGCCTCTTCAATCATCAACGGCTGTATTTTGTTCGCTTCAGCAACAAAGACATGAAAGGTCCCAATGGGTTCATCGCAGAGGCTCTGTATTGAGCTCCCTTACAACTAAAGCTGCTTCTTTGGAAGATGCAGTTGGTGCTGATGAAATTTTGAAGGCGGAAGCTGAGAAAGATGTACTCAAAGCATTGTCTCAGATCATCGATCCAGACTTTGGGACGGACATTGTCTCGTGTGGATTTGTGAAAGATCTGGACATCAATGATGCTTCGGGTGAGGTTTCATTTCGGTTAGAGCTCACTACACCAGCATGTCCAATAAAGGACATGTTTGAACAACAGGCAAATGAGGTGGTGAACGCTCTTCCCTGGGTCAAGAATGTCAATGTGACTATGTCAGCACAGCCAGAGAAACTGATTTATGCCGGGCAACTTCCGTTCTTTTCTGGAGGTGTGGGAAAATCAACTGTAGCGGTAGACTTAGCATACACTTTGGCTGGCATGGGTGCTAGAGTTGGTATATTTGATGCTGACGTCTATGGTCCAAGTTTACCGACAATGGTCTCCCCTGAGAACCGAATACTAGTGATGGTCGTCCAGCAGTTCGGTATTCCGAATCTGTTTGATCTTCCCATTAGACCAACTCTATCTGCTTCCGGAGATAGTGGAACGCCCGAAGTGGGAGCTGATCCTCTTGGTGAAGTTTCGAAGACATTTCAGGGTCTCGGAATATGTGTTGTGCAACAGTGTGCTAAGATTCGCCAACAAGTGTCAACGGCTGTGATGTATGATAAATCTATCAGGGCTACACAAGTGAAGGTACCGGATTCAGATGAAGAATTTCTTCTTCACTCTGCCCAAAGTGTGGATGAGTGGACAGGGGAGCAAAAGTTGCAGTTCGCTGATGTTCCGGAAGATATAGAACCTGAAGAGATTCGTCCCATGGGAAACTATGCAGTGTCAATAACGTGGTCAGACGGATTTAGTCAGATTGCTCCTTACGATCAGTTGCAGACAATAGAGCGGCTAGTTGATGTTCCTCGACTAGTTCCTTATCAGGCAAGTTACCATTCTTACCATAGTTTTTCACTGCATCAGCCTCAAATGGTAATAATAACAATGCTGTTTTTCCCTTCCCTCATACAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048037 GO:0051186 GO:0051536 GO:0051540 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF971 FeS_assembly_P ParA

Protein Analysis

413

Amino Acids

45.26

Weight (kDa)

4.7

Isoelectric Point (pI)

42.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FeS_assembly_P	PF01883	86 - 159	4.1e-20	Iron-sulfur cluster assembly protein
CbiA	PF01656	177 - 214	4e-07	CobQ/CobB/MinD/ParA nucleotide binding domain
ParA	PF10609	179 - 221	1.8e-14	NUBPL iron-transfer P-loop NTPase
GBBH-like_N	PF06155	314 - 359	9.3e-07	Gamma-butyrobetaine hydroxylase-like, N-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24430
fragaria_vesca	FvH4_1g05310 FvH4_1g05310 FvH4_1g05310 FvH4_1g05310
malus_domestica	MD02G1056300.v1.1 MD15G1189200.v1.1
prunus_persica	Prupe.7G227600_v2.0.a1
pyrus_communis	pycom15g16970
rosa_chinensis	RchiOBHm_Chr2g0091011
rosa_laevigata	RLG00000016166
rosa_multiflora	Rmu_sc0003305.1_g000005
rosa_roxburghii	Rroxscaffold_2G00150690
rosa_rugosa	Rorug02G0011400
rosa_samantha	Rh2AG057800 Rh2BG056100 Rh2CG058400 Rh2DG057500
rosa_wichuraiana	Rw2G005150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	624
Acc65I	GGTACC	1 cut(s)	907
AccB1I	GGYRCC	1 cut(s)	907
AccB7I	CCANNNNNTGG	2 cut(s)	302, 952
AccBSI	CCGCTC	1 cut(s)	1111
AccI	GTMKAC	1 cut(s)	564
AccIII	TCCGGA	2 cut(s)	743, 997
AciI	CCGC	3 cut(s)	248, 560, 1111
AclWI	GGATC	2 cut(s)	287, 770
AcsI	RAATTY	3 cut(s)	6, 237, 926
AcuI	CTGAAG	2 cut(s)	113, 1035
AcyI	GRCGYC	1 cut(s)	621
AfaI	GTAC	2 cut(s)	267, 909
AfiI	CCNNNNNNNGG	4 cut(s)	302, 767, 952, 1231
AflIII	ACRYGT	1 cut(s)	414
AgsI	TTSAA	4 cut(s)	44, 101, 244, 422
AhlI	ACTAGT	2 cut(s)	670, 1132
AjnI	CCWGG	1 cut(s)	455
AjuI	GAANNNNNNNTTGG	2 cut(s)	207, 239
AluBI	AGCT	5 cut(s)	186, 203, 254, 385, 773
AluI	AGCT	5 cut(s)	186, 203, 254, 385, 773
Alw21I	GWGCWC	2 cut(s)	188, 387
Alw26I	GTCTC	4 cut(s)	285, 322, 655, 815
AlwI	GGATC	2 cut(s)	287, 770
Aor13HI	TCCGGA	2 cut(s)	743, 997
ApeKI	GCWGC	1 cut(s)	203
ApoI	RAATTY	3 cut(s)	6, 237, 926
AseI	ATTAAT	1 cut(s)	74
Asp700I	GAANNNNTTC	3 cut(s)	927, 930, 1020
Asp718I	GGTACC	1 cut(s)	907
AspS9I	GGNCC	2 cut(s)	150, 629
AsuC2I	CCSGG	1 cut(s)	514
AsuHPI	GGTGA	3 cut(s)	374, 454, 797
AsuII	TTCGAA	1 cut(s)	794
AvaII	GGWCC	2 cut(s)	150, 629
BanI	GGYRCC	1 cut(s)	907
BanII	GRGCYC	2 cut(s)	188, 387
BauI	CACGAG	1 cut(s)	319
BbsI	GAAGAC	1 cut(s)	803
Bbv12I	GWGCWC	2 cut(s)	188, 387
BbvI	GCAGC	1 cut(s)	190
BccI	CCATC	1 cut(s)	670
BceAI	ACGGC	2 cut(s)	126, 879
BciT130I	CCWGG	1 cut(s)	457
BcnI	CCSGG	1 cut(s)	514
BcoDI	GTCTC	4 cut(s)	285, 322, 655, 815
BcuI	ACTAGT	2 cut(s)	670, 1132
BfaI	CTAG	4 cut(s)	597, 671, 1115, 1133
BfmI	CTRYAG	1 cut(s)	555
BglII	AGATCT	1 cut(s)	337
BisI	GCNGC	2 cut(s)	204, 1112
BlsI	GCNGC	2 cut(s)	205, 1113
Bme1390I	CCNGG	2 cut(s)	457, 514
Bme18I	GGWCC	2 cut(s)	150, 629
BmgT120I	GGNCC	2 cut(s)	150, 629
BmiI	GGNNCC	2 cut(s)	152, 909
BmrFI	CCNGG	2 cut(s)	457, 514
BmsI	GCATC	5 cut(s)	55, 208, 343, 604, 1189
BoxI	GACNNNNGTC	1 cut(s)	647
BpiI	GAAGAC	1 cut(s)	803
BpmI	CTGGAG	1 cut(s)	555
Bpu14I	TTCGAA	1 cut(s)	794
BpuMI	CCSGG	1 cut(s)	514
Bsa29I	ATCGAT	1 cut(s)	291
BsaBI	GATNNNNATC	1 cut(s)	76
BsaHI	GRCGYC	1 cut(s)	621
BsaI	GGTCTC	2 cut(s)	655, 815
BsaJI	CCNNGG	3 cut(s)	455, 456, 1029
BsaWI	WCCGGW	3 cut(s)	743, 910, 997
BsaXI	ACNNNNNCTCC	2 cut(s)	962, 992
Bsc4I	CCNNNNNNNGG	4 cut(s)	302, 767, 952, 1231
Bse8I	GATNNNNATC	1 cut(s)	76
BseAI	TCCGGA	2 cut(s)	743, 997
BseBI	CCWGG	1 cut(s)	457
BseCI	ATCGAT	1 cut(s)	291
BseDI	CCNNGG	3 cut(s)	455, 456, 1029
BseGI	GGATG	1 cut(s)	961
BseJI	GATNNNNATC	1 cut(s)	76
BseLI	CCNNNNNNNGG	4 cut(s)	302, 767, 952, 1231
BseMII	CTCAG	3 cut(s)	246, 296, 648
BseXI	GCAGC	1 cut(s)	190
BshNI	GGYRCC	1 cut(s)	907
BshVI	ATCGAT	1 cut(s)	291
BsiHKAI	GWGCWC	2 cut(s)	188, 387
BsiSI	CCGG	4 cut(s)	513, 744, 911, 998
BslFI	GGGAC	3 cut(s)	136, 319, 1011
BslI	CCNNNNNNNGG	4 cut(s)	302, 767, 952, 1231
BsmAI	GTCTC	4 cut(s)	285, 322, 655, 815
BsmFI	GGGAC	3 cut(s)	136, 319, 1011
Bso31I	GGTCTC	2 cut(s)	655, 815
Bsp119I	TTCGAA	1 cut(s)	794
Bsp1286I	GDGCHC	2 cut(s)	188, 387
Bsp13I	TCCGGA	2 cut(s)	743, 997
Bsp143I	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
Bsp19I	CCATGG	1 cut(s)	1029
BspACI	CCGC	3 cut(s)	248, 560, 1111
BspCNI	CTCAG	3 cut(s)	247, 295, 649
BspDI	ATCGAT	1 cut(s)	291
BspEI	TCCGGA	2 cut(s)	743, 997
BspHI	TCATGA	1 cut(s)	58
BspLI	GGNNCC	2 cut(s)	152, 909
BspPI	GGATC	2 cut(s)	287, 770
BspQI	GCTCTTC	1 cut(s)	456
BspT104I	TTCGAA	1 cut(s)	794
BspT107I	GGYRCC	1 cut(s)	907
BspTNI	GGTCTC	2 cut(s)	655, 815
BsrBI	CCGCTC	1 cut(s)	1111
BssECI	CCNNGG	3 cut(s)	455, 456, 1029
BssMI	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
BssNI	GRCGYC	1 cut(s)	621
BssSI	CACGAG	1 cut(s)	319
BssT1I	CCWWGG	1 cut(s)	1029
Bst2BI	CACGAG	1 cut(s)	319
Bst2UI	CCWGG	1 cut(s)	457
Bst4CI	ACNGT	2 cut(s)	556, 834
Bst6I	CTCTTC	3 cut(s)	102, 456, 1011
BstACI	GRCGYC	1 cut(s)	621
BstAPI	GCANNNNNTGC	2 cut(s)	227, 979
BstBI	TTCGAA	1 cut(s)	794
BstC8I	GCNNGC	1 cut(s)	586
BstDEI	CTNAG	5 cut(s)	255, 282, 568, 657, 840
BstDSI	CCRYGG	1 cut(s)	1029
BstENI	CCTNNNNNAGG	1 cut(s)	1229
BstF5I	GGATG	1 cut(s)	961
BstKTI	GATC	6 cut(s)	288, 295, 340, 715, 778, 1093
BstMAI	GTCTC	4 cut(s)	285, 322, 655, 815
BstMBI	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
BstMWI	GCNNNNNNNGC	2 cut(s)	227, 979
BstNI	CCWGG	1 cut(s)	457
BstNSI	RCATGY	2 cut(s)	402, 418
BstPAI	GACNNNNGTC	1 cut(s)	647
BstSCI	CCNGG	2 cut(s)	455, 512
BstSFI	CTRYAG	1 cut(s)	555
BstV1I	GCAGC	1 cut(s)	190
BstV2I	GAAGAC	1 cut(s)	803
BstX2I	RGATCY	1 cut(s)	337
BstYI	RGATCY	1 cut(s)	337
Bsu15I	ATCGAT	1 cut(s)	291
BsuTUI	ATCGAT	1 cut(s)	291
BtgI	CCRYGG	1 cut(s)	1029
BtgZI	GCGATG	1 cut(s)	149
BtsCI	GGATG	1 cut(s)	961
BtsI	GCAGTG	2 cut(s)	1050, 1175
BtsIMutI	CAGTG	3 cut(s)	839, 1050, 1175
Cac8I	GCNNGC	1 cut(s)	586
CciI	TCATGA	1 cut(s)	58
Cfr13I	GGNCC	2 cut(s)	150, 629
ClaI	ATCGAT	1 cut(s)	291
Csp6I	GTAC	2 cut(s)	266, 908
CspCI	CAANNNNNGTGG	2 cut(s)	420, 455
CviAII	CATG	6 cut(s)	59, 143, 399, 415, 589, 1030
CviQI	GTAC	2 cut(s)	266, 908
DdeI	CTNAG	5 cut(s)	255, 282, 568, 657, 840
DpnI	GATC	6 cut(s)	287, 294, 339, 714, 777, 1092
DpnII	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
Eam1104I	CTCTTC	3 cut(s)	102, 456, 1011
EarI	CTCTTC	3 cut(s)	102, 456, 1011
EciI	GGCGGA	1 cut(s)	263
Ecl136II	GAGCTC	2 cut(s)	186, 385
Eco130I	CCWWGG	1 cut(s)	1029
Eco24I	GRGCYC	2 cut(s)	188, 387
Eco31I	GGTCTC	2 cut(s)	655, 815
Eco47I	GGWCC	2 cut(s)	150, 629
Eco53kI	GAGCTC	2 cut(s)	186, 385
Eco57I	CTGAAG	2 cut(s)	113, 1035
EcoICRI	GAGCTC	2 cut(s)	186, 385
EcoNI	CCTNNNNNAGG	1 cut(s)	1229
EcoO109I	RGGNCCY	1 cut(s)	150
EcoRII	CCWGG	1 cut(s)	455
EcoT14I	CCWWGG	1 cut(s)	1029
EcoT38I	GRGCYC	2 cut(s)	188, 387
ErhI	CCWWGG	1 cut(s)	1029
FaeI	CATG	6 cut(s)	62, 146, 402, 418, 592, 1033
FalI	AAGNNNNNCTT	2 cut(s)	915, 947
FaqI	GGGAC	3 cut(s)	136, 319, 1011
FatI	CATG	6 cut(s)	58, 142, 398, 414, 588, 1029
FblI	GTMKAC	1 cut(s)	564
Fnu4HI	GCNGC	2 cut(s)	204, 1112
FokI	GGATG	1 cut(s)	968
FriOI	GRGCYC	2 cut(s)	188, 387
Fsp4HI	GCNGC	2 cut(s)	204, 1112
FspBI	CTAG	4 cut(s)	597, 671, 1115, 1133
GluI	GCNGC	2 cut(s)	204, 1112
GsuI	CTGGAG	1 cut(s)	555
HapII	CCGG	4 cut(s)	513, 744, 911, 998
Hin1I	GRCGYC	1 cut(s)	621
Hin1II	CATG	6 cut(s)	62, 146, 402, 418, 592, 1033
HincII	GTYRAC	1 cut(s)	861
HindII	GTYRAC	1 cut(s)	861
HinfI	GANTC	4 cut(s)	703, 844, 914, 1021
HpaII	CCGG	4 cut(s)	513, 744, 911, 998
HphI	GGTGA	3 cut(s)	374, 454, 797
Hpy166II	GTNNAC	5 cut(s)	445, 565, 638, 861, 963
Hpy188I	TCNGA	6 cut(s)	285, 702, 815, 919, 1063, 1077
Hpy188III	TCNNGA	7 cut(s)	59, 296, 341, 463, 534, 744, 998
Hpy8I	GTNNAC	5 cut(s)	445, 565, 638, 861, 963
HpyAV	CCTTC	4 cut(s)	30, 238, 898, 1230
HpyCH4III	ACNGT	2 cut(s)	556, 834
HpyCH4IV	ACGT	4 cut(s)	15, 48, 621, 1055
HpyCH4V	TGCA	8 cut(s)	4, 68, 221, 829, 982, 1043, 1099, 1180
HpyF10VI	GCNNNNNNNGC	2 cut(s)	227, 979
HpyF3I	CTNAG	5 cut(s)	255, 282, 568, 657, 840
HpySE526I	ACGT	4 cut(s)	15, 48, 621, 1055
Hsp92I	GRCGYC	1 cut(s)	621
Hsp92II	CATG	6 cut(s)	62, 146, 402, 418, 592, 1033
Kpn2I	TCCGGA	2 cut(s)	743, 997
KpnI	GGTACC	1 cut(s)	911
Kzo9I	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
LguI	GCTCTTC	1 cut(s)	456
LmnI	GCTCC	4 cut(s)	191, 770, 970, 1087
Lsp1109I	GCAGC	1 cut(s)	190
LweI	GCATC	5 cut(s)	55, 208, 343, 604, 1189
MaeI	CTAG	4 cut(s)	597, 671, 1115, 1133
MaeII	ACGT	4 cut(s)	15, 48, 621, 1055
MaeIII	GTNAC	2 cut(s)	475, 1151
MalI	GATC	6 cut(s)	287, 294, 339, 714, 777, 1092
MbiI	CCGCTC	1 cut(s)	1111
MboI	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
MflI	RGATCY	1 cut(s)	337
MhlI	GDGCHC	2 cut(s)	188, 387
MluCI	AATT	3 cut(s)	6, 237, 926
MnlI	CCTC	9 cut(s)	105, 164, 358, 430, 530, 789, 1137, 1197, 1235
MroI	TCCGGA	2 cut(s)	743, 997
MroXI	GAANNNNTTC	3 cut(s)	927, 930, 1020
MseI	TTAA	1 cut(s)	74
MslI	CAYNNNNRTG	2 cut(s)	63, 397
MspI	CCGG	4 cut(s)	513, 744, 911, 998
MspR9I	CCNGG	2 cut(s)	457, 514
MvaI	CCWGG	1 cut(s)	457
MwoI	GCNNNNNNNGC	2 cut(s)	227, 979
NciI	CCSGG	1 cut(s)	514
NcoI	CCATGG	1 cut(s)	1029
NdeII	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
NlaIII	CATG	6 cut(s)	62, 146, 402, 418, 592, 1033
NlaIV	GGNNCC	2 cut(s)	152, 909
NmuCI	GTSAC	1 cut(s)	475
NspI	RCATGY	2 cut(s)	402, 418
NspV	TTCGAA	1 cut(s)	794
PagI	TCATGA	1 cut(s)	58
PasI	CCCWGGG	1 cut(s)	456
PciI	ACATGT	1 cut(s)	414
PciSI	GCTCTTC	1 cut(s)	456
PdmI	GAANNNNTTC	3 cut(s)	927, 930, 1020
PfeI	GAWTC	4 cut(s)	703, 844, 914, 1021
PflFI	GACNNNGTC	2 cut(s)	314, 481
PflMI	CCANNNNNTGG	2 cut(s)	302, 952
PkrI	GCNGC	2 cut(s)	205, 1113
PpuMI	RGGWCCY	1 cut(s)	150
PscI	ACATGT	1 cut(s)	414
PshAI	GACNNNNGTC	1 cut(s)	647
PshBI	ATTAAT	1 cut(s)	74
Psp124BI	GAGCTC	2 cut(s)	188, 387
Psp5II	RGGWCCY	1 cut(s)	150
Psp6I	CCWGG	1 cut(s)	455
PspGI	CCWGG	1 cut(s)	455
PspN4I	GGNNCC	2 cut(s)	152, 909
PspPI	GGNCC	2 cut(s)	150, 629
PspPPI	RGGWCCY	1 cut(s)	150
PsuI	RGATCY	1 cut(s)	337
PsyI	GACNNNGTC	2 cut(s)	314, 481
RsaI	GTAC	2 cut(s)	267, 909
RsaNI	GTAC	2 cut(s)	266, 908
RseI	CAYNNNNRTG	2 cut(s)	63, 397
SacI	GAGCTC	2 cut(s)	188, 387
SapI	GCTCTTC	1 cut(s)	456
SaqAI	TTAA	1 cut(s)	74
SatI	GCNGC	2 cut(s)	204, 1112
Sau3AI	GATC	6 cut(s)	285, 292, 337, 712, 775, 1090
Sau96I	GGNCC	2 cut(s)	150, 629
ScrFI	CCNGG	2 cut(s)	457, 514
SduI	GDGCHC	2 cut(s)	188, 387
SfaNI	GCATC	5 cut(s)	55, 208, 343, 604, 1189
SfcI	CTRYAG	1 cut(s)	555
SfuI	TTCGAA	1 cut(s)	794
SinI	GGWCC	2 cut(s)	150, 629
SmiMI	CAYNNNNRTG	2 cut(s)	63, 397
SpeI	ACTAGT	2 cut(s)	670, 1132
Sse9I	AATT	3 cut(s)	6, 237, 926
SsiI	CCGC	3 cut(s)	248, 560, 1111
SspMI	CTAG	4 cut(s)	597, 671, 1115, 1133
SstI	GAGCTC	2 cut(s)	188, 387
StyD4I	CCNGG	2 cut(s)	455, 512
StyI	CCWWGG	1 cut(s)	1029
TaaI	ACNGT	2 cut(s)	556, 834
TaiI	ACGT	4 cut(s)	18, 51, 624, 1058
TaqI	TCGA	3 cut(s)	291, 794, 1129
TasI	AATT	3 cut(s)	6, 237, 926
TatI	WGTACW	1 cut(s)	265
TauI	GCSGC	1 cut(s)	1114
TfiI	GAWTC	4 cut(s)	703, 844, 914, 1021
Tru1I	TTAA	1 cut(s)	74
Tru9I	TTAA	1 cut(s)	74
TscAI	CASTG	3 cut(s)	839, 1050, 1182
TseFI	GTSAC	1 cut(s)	475
TseI	GCWGC	1 cut(s)	203
Tsp45I	GTSAC	1 cut(s)	475
TspDTI	ATGAA	6 cut(s)	75, 152, 159, 249, 360, 936
TspGWI	ACGGA	3 cut(s)	323, 513, 1080
TspRI	CASTG	3 cut(s)	839, 1050, 1182
Tth111I	GACNNNGTC	2 cut(s)	314, 481
Van91I	CCANNNNNTGG	2 cut(s)	302, 952
VpaK11BI	GGWCC	2 cut(s)	150, 629
VspI	ATTAAT	1 cut(s)	74
XagI	CCTNNNNNAGG	1 cut(s)	1229
XapI	RAATTY	3 cut(s)	6, 237, 926
XceI	RCATGY	2 cut(s)	402, 418
XmiI	GTMKAC	1 cut(s)	564
XmnI	GAANNNNTTC	3 cut(s)	927, 930, 1020
XspI	CTAG	4 cut(s)	597, 671, 1115, 1133
ZraI	GACGTC	1 cut(s)	622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.