MD02G1059600.v1.1

Glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013819
Physical Location & Seq
Forward (+)
4782798 .. 4785295
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1059600.v1.1.491

Sequence Viewer

Length: 1788 bp
ATGTCAAAGTCTGGCAAGTTTAGTGTTGGTGGTGTAGAGGGCCTGGTGGGTAATGACACTGAAGTTTCCGATTCGGAGGAAGAAACTAGGCATGGGAATGATACGAGACAACAGACCTGGGTGTATGGATTAGCACTAGGTAATGACAGCAGTTCGACCGGGGAGTCCATGTTAGAGAATAATGTAACACTTGGAAAGAGTTATCCGGTGGGAACGGGTGGAAATGAAGATGGTATCTTTATCCGGGGAAAGACAGATTTTGAAAATGGTTACTTACAGACAGCTAATGAATCAGTTGACGGTTACAGGGAAGACAACGACCCTGGAATCAGCTCTGGTTTGAAGGTTAATGAAGTTAGAAATGAAGTTGGTCTTGTACCAGTTGTATCACAAGAAAGCTCTACAAAGAGACCGGAAAACTTGAATGTAGATTCAAAGGGCAATGTCGAACAAAATACAGAAACCCAAATTGATCACCAGAAGATTGAGCTACAGCAACCTGTTTCAGTCACCTTGGACGATAATTCTACAATGACTGTAGATTCTGTACTAAGGAAGTGGAATCGACGACCAACATCAATATCACAGATAAACTCATTATTGCTTCAAAGTGCTGTTTCTTCTCGTTCAATGAAACTGCGGAGGTACTCAGCACGTGATCGTGAACTACAATCTGCAAAACTGGAGATTGAGAATGCTCCCATCATAAGGGACAATGCAGGACTTAGTGCTTCTGTTTTCCGAAATCTCTCCAAATTTATAAGGAGTTATGACTTGATGGGGCACATGCTCAAAGTTTACATCTACAGTGAAGGTGAAAAGCCGGTATTCCATCAGCCTTTGATGAGAGGCATCTATGCCTCAGAAGGCTGGTTTATGAAACTAATTGAAGGAAATAAAAAATTTGTTGTGAGGGACCCCAAGAAAGCTCATTTATTCTATTTACCTTTCGATTCGCATATGCTAAGGTTGACTATTCCTAGACACTCTATCCATGGTAAGAGTAAGACAGTCCTAGAAGAAGTTTTGCAGAGCTACGTTGGTTTAATTTCTAGAAAGTATCCTTTCTGGAATAGAACGAAAGGGGCAGATCATTTTCTTGTTGCTTGTCATGACTGGGCCCCCAAGCTCACAAAACAGTGCATGAAGAATTGCATCAGGTCTCTTTGCAACGCACATGTCGATAGAGAATTCGAAATTGGCAAGGACACCAGTCTACCTGTGACATATATACGTTCCGTGGAGAATCCTCTTCAGGATCTTGGAGGAAAACCTGCTTCAGAAAGATCTATCCTGGCCTTCTTTGCCGGGGGCTTGCATGGCTATCTCCGACCAATCCTGCTACACTACTGGGCAAACAAAGAACCCGACATGAAAATCTTTGGCCCAATGCCGCATGATATTGAAGGCAAAAGAATGTATAGGGAATACATGAAGAGCAGCAAGTTTTGCATATGTGCCAGGGGTTACGAGGTCCACACGCCTCGAATAGTTGAGGCCATATTCTACGAGTGTGTGCCAGTCATCATATCAGACAGTTACATGCCTCCTTTCTTTGAAGTATTTAATTGGGAGGCATTTGCAGTTTTCATACAAGAGAGGGACATCCCCAATTTGAGAAACATTCTGCTCTCAATCCCGGAAGACAAGTACCTTACAATGATGTCGAGAATAAAAATGGTGCAACAACATTTCTTCTGGCACAAGAAACCCAAGAAGTACGACTTATTTCATATGGTACTTCATTCAGTCTGGTACAACAGAATTCTTCAGTTAAAAACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

596

Amino Acids

68.19

Weight (kDa)

8.67

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 262 - 544 7.6e-63 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AasI GACNNNNNNGTC 1 cut(s) 163
Acc36I ACCTGC 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 1216
AciI CCGC 2 cut(s) 640, 1394
AclWI GGATC 1 cut(s) 1266
AcsI RAATTY 4 cut(s) 755, 902, 1190, 1764
AcuI CTGAAG 4 cut(s) 81, 1238, 1263, 1754
AcvI CACGTG 1 cut(s) 656
AfaI GTAC 7 cut(s) 378, 549, 647, 1652, 1721, 1740, 1757
AfiI CCNNNNNNNGG 1 cut(s) 708
AflIII ACRYGT 1 cut(s) 1177
AgsI TTSAA 9 cut(s) 263, 343, 424, 435, 608, 630, 890, 1406, 1559
AjnI CCWGG 5 cut(s) 42, 116, 322, 1293, 1460
AjuI GAANNNNNNNTTGG 2 cut(s) 1182, 1214
AluBI AGCT 7 cut(s) 284, 333, 399, 490, 929, 1035, 1129
AluI AGCT 7 cut(s) 284, 333, 399, 490, 929, 1035, 1129
Alw26I GTCTC 3 cut(s) 100, 403, 1167
AlwI GGATC 1 cut(s) 1266
AoxI GGCC 5 cut(s) 40, 1119, 1296, 1384, 1497
ApaI GGGCCC 1 cut(s) 1123
ApeKI GCWGC 1 cut(s) 1440
ApoI RAATTY 4 cut(s) 755, 902, 1190, 1764
AspS9I GGNCC 6 cut(s) 40, 916, 1119, 1120, 1385, 1474
AsuC2I CCSGG 4 cut(s) 160, 245, 1309, 1640
AsuHPI GGTGA 3 cut(s) 467, 502, 827
AsuII TTCGAA 1 cut(s) 1194
AvaII GGWCC 2 cut(s) 916, 1474
BaeGI GKGCMC 2 cut(s) 786, 1123
BanII GRGCYC 1 cut(s) 1123
BbrPI CACGTG 1 cut(s) 656
BbsI GAAGAC 2 cut(s) 318, 1650
BbvI GCAGC 1 cut(s) 1452
BccI CCATC 4 cut(s) 224, 710, 772, 840
BciT130I CCWGG 5 cut(s) 44, 118, 324, 1295, 1462
BciVI GTATCC 1 cut(s) 1071
BclI TGATCA 1 cut(s) 472
BcnI CCSGG 4 cut(s) 160, 245, 1309, 1640
BcoDI GTCTC 3 cut(s) 100, 403, 1167
BfaI CTAG 5 cut(s) 87, 137, 981, 1016, 1053
BfmI CTRYAG 3 cut(s) 491, 537, 805
BfuAI ACCTGC 1 cut(s) 1282
BfuI GTATCC 1 cut(s) 1071
BglII AGATCT 1 cut(s) 1286
BisI GCNGC 2 cut(s) 1394, 1441
BlsI GCNGC 2 cut(s) 1395, 1442
Bme1390I CCNGG 9 cut(s) 44, 118, 160, 245, 324, 1295, 1309, 1462, 1640
Bme18I GGWCC 2 cut(s) 916, 1474
BmgT120I GGNCC 6 cut(s) 40, 916, 1119, 1120, 1385, 1474
BmiI GGNNCC 4 cut(s) 917, 918, 1121, 1122
BmrFI CCNGG 9 cut(s) 44, 118, 160, 245, 324, 1295, 1309, 1462, 1640
BmrI ACTGGG 2 cut(s) 1126, 1360
BmsI GCATC 2 cut(s) 861, 1164
BmuI ACTGGG 2 cut(s) 1126, 1360
BoxI GACNNNNGTC 1 cut(s) 1212
BpiI GAAGAC 2 cut(s) 318, 1650
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 965
Bpu14I TTCGAA 1 cut(s) 1194
BpuMI CCSGG 4 cut(s) 160, 245, 1309, 1640
BsaAI YACGTR 1 cut(s) 656
BsaI GGTCTC 2 cut(s) 403, 1167
BsaJI CCNNGG 9 cut(s) 117, 159, 244, 322, 513, 994, 1239, 1308, 1461
BsaWI WCCGGW 2 cut(s) 205, 412
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 708
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 6 cut(s) 380, 687, 1121, 1212, 1355, 1520
Bse3DI GCAATG 1 cut(s) 448
BseBI CCWGG 5 cut(s) 44, 118, 324, 1295, 1462
BseDI CCNNGG 9 cut(s) 117, 159, 244, 322, 513, 994, 1239, 1308, 1461
BseGI GGATG 1 cut(s) 1605
BseLI CCNNNNNNNGG 1 cut(s) 708
BseMI GCAATG 1 cut(s) 448
BseMII CTCAG 2 cut(s) 663, 876
BseNI ACTGG 6 cut(s) 380, 687, 1121, 1212, 1355, 1520
BseSI GKGCMC 2 cut(s) 786, 1123
BseXI GCAGC 1 cut(s) 1452
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 5 cut(s) 42, 1121, 1298, 1386, 1499
BsiEI CGRYCG 1 cut(s) 159
BsiSI CCGG 7 cut(s) 159, 206, 244, 413, 824, 1308, 1640
BslFI GGGAC 3 cut(s) 725, 929, 1616
BslI CCNNNNNNNGG 1 cut(s) 708
BsmAI GTCTC 3 cut(s) 100, 403, 1167
BsmFI GGGAC 3 cut(s) 725, 929, 1616
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 5 cut(s) 42, 1121, 1298, 1386, 1499
Bso31I GGTCTC 2 cut(s) 403, 1167
Bsp119I TTCGAA 1 cut(s) 1194
Bsp120I GGGCCC 1 cut(s) 1119
Bsp1286I GDGCHC 2 cut(s) 786, 1123
Bsp143I GATC 5 cut(s) 472, 658, 1090, 1258, 1286
Bsp19I CCATGG 1 cut(s) 994
BspACI CCGC 2 cut(s) 640, 1394
BspANI GGCC 5 cut(s) 42, 1121, 1298, 1386, 1499
BspCNI CTCAG 2 cut(s) 662, 875
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 4 cut(s) 917, 918, 1121, 1122
BspMI ACCTGC 1 cut(s) 1282
BspPI GGATC 1 cut(s) 1266
BspQI GCTCTTC 1 cut(s) 1430
BspT104I TTCGAA 1 cut(s) 1194
BspTNI GGTCTC 2 cut(s) 403, 1167
BsrDI GCAATG 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 6 cut(s) 380, 687, 1121, 1212, 1355, 1520
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 9 cut(s) 117, 159, 244, 322, 513, 994, 1239, 1308, 1461
BssMI GATC 5 cut(s) 472, 658, 1090, 1258, 1286
BssT1I CCWWGG 2 cut(s) 513, 994
Bst2UI CCWGG 5 cut(s) 44, 118, 324, 1295, 1462
Bst4CI ACNGT 6 cut(s) 302, 538, 809, 1012, 1140, 1538
Bst6I CTCTTC 2 cut(s) 1257, 1430
BstAPI GCANNNNNTGC 1 cut(s) 1449
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 1194
BstC8I GCNNGC 1 cut(s) 1316
BstDEI CTNAG 5 cut(s) 551, 649, 725, 862, 965
BstDSI CCRYGG 2 cut(s) 994, 1239
BstF5I GGATG 1 cut(s) 1605
BstKTI GATC 5 cut(s) 475, 661, 1093, 1261, 1289
BstMAI GTCTC 3 cut(s) 100, 403, 1167
BstMBI GATC 5 cut(s) 472, 658, 1090, 1258, 1286
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 3 cut(s) 1304, 1320, 1449
BstNI CCWGG 5 cut(s) 44, 118, 324, 1295, 1462
BstNSI RCATGY 3 cut(s) 790, 1181, 1546
BstPAI GACNNNNGTC 1 cut(s) 1212
BstSCI CCNGG 9 cut(s) 42, 116, 158, 243, 322, 1293, 1307, 1460, 1638
BstSFI CTRYAG 3 cut(s) 491, 537, 805
BstSLI GKGCMC 2 cut(s) 786, 1123
BstV1I GCAGC 1 cut(s) 1452
BstV2I GAAGAC 2 cut(s) 318, 1650
BstX2I RGATCY 2 cut(s) 1258, 1286
BstYI RGATCY 2 cut(s) 1258, 1286
BsuI GTATCC 1 cut(s) 1071
BsuRI GGCC 5 cut(s) 42, 1121, 1298, 1386, 1499
BtgI CCRYGG 2 cut(s) 994, 1239
BtsCI GGATG 1 cut(s) 1605
BtsIMutI CAGTG 3 cut(s) 57, 814, 1145
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 1 cut(s) 1316
CciI TCATGA 1 cut(s) 1111
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 6 cut(s) 40, 916, 1119, 1120, 1385, 1474
Csp6I GTAC 7 cut(s) 377, 548, 646, 1651, 1720, 1739, 1756
CviQI GTAC 7 cut(s) 377, 548, 646, 1651, 1720, 1739, 1756
DdeI CTNAG 5 cut(s) 551, 649, 725, 862, 965
DpnI GATC 5 cut(s) 474, 660, 1092, 1260, 1288
DpnII GATC 5 cut(s) 472, 658, 1090, 1258, 1286
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 1257, 1430
EarI CTCTTC 2 cut(s) 1257, 1430
Eco130I CCWWGG 2 cut(s) 513, 994
Eco24I GRGCYC 1 cut(s) 1123
Eco31I GGTCTC 2 cut(s) 403, 1167
Eco47I GGWCC 2 cut(s) 916, 1474
Eco57I CTGAAG 4 cut(s) 81, 1238, 1263, 1754
Eco72I CACGTG 1 cut(s) 656
EcoO109I RGGNCCY 3 cut(s) 40, 916, 1120
EcoRI GAATTC 2 cut(s) 1190, 1764
EcoRII CCWGG 5 cut(s) 42, 116, 322, 1293, 1460
EcoT14I CCWWGG 2 cut(s) 513, 994
EcoT38I GRGCYC 1 cut(s) 1123
ErhI CCWWGG 2 cut(s) 513, 994
FalI AAGNNNNNCTT 4 cut(s) 357, 389, 1709, 1741
FaqI GGGAC 3 cut(s) 725, 929, 1616
FauNDI CATATG 3 cut(s) 960, 1454, 1734
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 1216
Fnu4HI GCNGC 2 cut(s) 1394, 1441
FokI GGATG 1 cut(s) 1592
FriOI GRGCYC 1 cut(s) 1123
Fsp4HI GCNGC 2 cut(s) 1394, 1441
FspBI CTAG 5 cut(s) 87, 137, 981, 1016, 1053
GluI GCNGC 2 cut(s) 1394, 1441
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 5 cut(s) 42, 1121, 1298, 1386, 1499
HapII CCGG 7 cut(s) 159, 206, 244, 413, 824, 1308, 1640
HincII GTYRAC 2 cut(s) 298, 972
HindII GTYRAC 2 cut(s) 298, 972
HinfI GANTC 9 cut(s) 71, 164, 290, 327, 431, 542, 562, 953, 1246
HpaII CCGG 7 cut(s) 159, 206, 244, 413, 824, 1308, 1640
HphI GGTGA 3 cut(s) 467, 502, 827
Hpy166II GTNNAC 6 cut(s) 298, 665, 799, 972, 1217, 1477
Hpy188I TCNGA 7 cut(s) 70, 76, 743, 865, 1282, 1331, 1534
Hpy188III TCNNGA 6 cut(s) 662, 1053, 1069, 1112, 1256, 1668
Hpy8I GTNNAC 6 cut(s) 298, 665, 799, 972, 1217, 1477
Hpy99I CGWCG 1 cut(s) 570
HpyAV CCTTC 6 cut(s) 337, 806, 860, 884, 1309, 1400
HpyCH4III ACNGT 6 cut(s) 302, 538, 809, 1012, 1140, 1538
HpyCH4IV ACGT 3 cut(s) 655, 1038, 1234
HpyF10VI GCNNNNNNNGC 3 cut(s) 1304, 1320, 1449
HpyF3I CTNAG 5 cut(s) 551, 649, 725, 862, 965
HpySE526I ACGT 3 cut(s) 655, 1038, 1234
KflI GGGWCCC 1 cut(s) 916
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 5 cut(s) 472, 658, 1090, 1258, 1286
LguI GCTCTTC 1 cut(s) 1430
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 1 cut(s) 1452
LweI GCATC 2 cut(s) 861, 1164
MaeI CTAG 5 cut(s) 87, 137, 981, 1016, 1053
MaeII ACGT 3 cut(s) 655, 1038, 1234
MaeIII GTNAC 7 cut(s) 184, 269, 302, 508, 1222, 1466, 1538
MalI GATC 5 cut(s) 474, 660, 1092, 1260, 1288
MboI GATC 5 cut(s) 472, 658, 1090, 1258, 1286
MflI RGATCY 2 cut(s) 1258, 1286
MhlI GDGCHC 2 cut(s) 786, 1123
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 1354
MseI TTAA 4 cut(s) 348, 1046, 1566, 1775
MslI CAYNNNNRTG 1 cut(s) 96
MspI CCGG 7 cut(s) 159, 206, 244, 413, 824, 1308, 1640
MspR9I CCNGG 9 cut(s) 44, 118, 160, 245, 324, 1295, 1309, 1462, 1640
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 5 cut(s) 44, 118, 324, 1295, 1462
MwoI GCNNNNNNNGC 3 cut(s) 1304, 1320, 1449
NciI CCSGG 4 cut(s) 160, 245, 1309, 1640
NcoI CCATGG 1 cut(s) 994
NdeI CATATG 3 cut(s) 960, 1454, 1734
NdeII GATC 5 cut(s) 472, 658, 1090, 1258, 1286
NlaIV GGNNCC 4 cut(s) 917, 918, 1121, 1122
NmuCI GTSAC 2 cut(s) 508, 1222
NspI RCATGY 3 cut(s) 790, 1181, 1546
NspV TTCGAA 1 cut(s) 1194
PagI TCATGA 1 cut(s) 1111
PciI ACATGT 1 cut(s) 1177
PciSI GCTCTTC 1 cut(s) 1430
PcsI WCGNNNNNNNCGW 1 cut(s) 1179
PctI GAATGC 1 cut(s) 700
PfeI GAWTC 8 cut(s) 71, 290, 327, 431, 542, 562, 953, 1246
PfoI TCCNGGA 1 cut(s) 1638
PkrI GCNGC 2 cut(s) 1395, 1442
PleI GAGTC 1 cut(s) 172
PmaCI CACGTG 1 cut(s) 656
PmlI CACGTG 1 cut(s) 656
PpsI GAGTC 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 656
PpuMI RGGWCCY 1 cut(s) 916
PscI ACATGT 1 cut(s) 1177
PshAI GACNNNNGTC 1 cut(s) 1212
PsiI TTATAA 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 916
Psp6I CCWGG 5 cut(s) 42, 116, 322, 1293, 1460
PspCI CACGTG 1 cut(s) 656
PspGI CCWGG 5 cut(s) 42, 116, 322, 1293, 1460
PspN4I GGNNCC 4 cut(s) 917, 918, 1121, 1122
PspOMI GGGCCC 1 cut(s) 1119
PspPI GGNCC 6 cut(s) 40, 916, 1119, 1120, 1385, 1474
PspPPI RGGWCCY 1 cut(s) 916
PsuI RGATCY 2 cut(s) 1258, 1286
RsaI GTAC 7 cut(s) 378, 549, 647, 1652, 1721, 1740, 1757
RsaNI GTAC 7 cut(s) 377, 548, 646, 1651, 1720, 1739, 1756
RseI CAYNNNNRTG 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 1430
SaqAI TTAA 4 cut(s) 348, 1046, 1566, 1775
SatI GCNGC 2 cut(s) 1394, 1441
Sau3AI GATC 5 cut(s) 472, 658, 1090, 1258, 1286
Sau96I GGNCC 6 cut(s) 40, 916, 1119, 1120, 1385, 1474
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 9 cut(s) 44, 118, 160, 245, 324, 1295, 1309, 1462, 1640
SduI GDGCHC 2 cut(s) 786, 1123
SfaNI GCATC 2 cut(s) 861, 1164
SfcI CTRYAG 3 cut(s) 491, 537, 805
SfuI TTCGAA 1 cut(s) 1194
SinI GGWCC 2 cut(s) 916, 1474
SmiMI CAYNNNNRTG 1 cut(s) 96
SsiI CCGC 2 cut(s) 640, 1394
SspMI CTAG 5 cut(s) 87, 137, 981, 1016, 1053
StyD4I CCNGG 9 cut(s) 42, 116, 158, 243, 322, 1293, 1307, 1460, 1638
StyI CCWWGG 2 cut(s) 513, 994
TaaI ACNGT 6 cut(s) 302, 538, 809, 1012, 1140, 1538
TaiI ACGT 3 cut(s) 658, 1041, 1237
TaqI TCGA 8 cut(s) 155, 447, 565, 951, 1182, 1194, 1486, 1667
TatI WGTACW 1 cut(s) 547
TauI GCSGC 1 cut(s) 1396
TfiI GAWTC 8 cut(s) 71, 290, 327, 431, 542, 562, 953, 1246
Tru1I TTAA 4 cut(s) 348, 1046, 1566, 1775
Tru9I TTAA 4 cut(s) 348, 1046, 1566, 1775
TscAI CASTG 3 cut(s) 64, 814, 1145
TseFI GTSAC 2 cut(s) 508, 1222
TseI GCWGC 1 cut(s) 1440
Tsp45I GTSAC 2 cut(s) 508, 1222
TspGWI ACGGA 1 cut(s) 1228
TspRI CASTG 3 cut(s) 64, 814, 1145
VpaK11BI GGWCC 2 cut(s) 916, 1474
XapI RAATTY 4 cut(s) 755, 902, 1190, 1764
XbaI TCTAGA 1 cut(s) 1052
XceI RCATGY 3 cut(s) 790, 1181, 1546
XmiI GTMKAC 1 cut(s) 1216
XspI CTAG 5 cut(s) 87, 137, 981, 1016, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.