MD02G1067500.v1.1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012782
Physical Location & Seq
Reverse (-)
5516193 .. 5519563
3371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1067500.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGAAGACGAAGACGCCATTGAATTTGAACTTGAAGCAGCTCAAGCTCAATGTCCCTGCTCAAGAAGCCAACATCAAGTCCTTTCTGACTGCCAGTGGCACATTTCATGACGGCGATCTGCGTCTGAACCAGAAAGGATTGCGGCTTATCTCTGAAGAAAAGGAGACCCCAGATCAAACTTCTGACAGTAAGGAGCTCAACTTTGAAATTACATTGGAAGATCTTGAGACTATCAAAGTGATCGGGAAGGGAAGTGGTGGTGTAGTACAACTTGTTCGTCATAAATGGGTCGGAAAACTATTTGCCTTGAAGGTGATTCAGATGAACATACAAGAGGAAATTCGTAAACAGATTGTGCAGGAGCTAAAAATAAACCAAGCAGCACAATGTCCACATGTGGTGGTTTGTCACCACTCATTCTATCACAACGGGGCCATTTCTCTTGTGTTAGAATATATGGACCGGGGGTCTCTTGCAGATGTGATCAGACAAGTTAACACAATTCTTGAACCATATCTTGCAGTCGTTTGTAAACAGGTTTTACAAGGTCTAGTGTACTTGCATAATGAAAGACATGTGATACATAGAGACATAAAACCATCCAATCTGCTAGTAAACCATCAAGGGCAGGTGAAGATCACCGATTTTGGCGTGAGTGCTTCGCTAGCTAGCTCTATGGGTCAAAGAGACACATTCGTTGGTACTTACAATTACATGTCGCCGGAGAGGATTAGCGGGAGTACTTATGACTATAGCAGTGATATTTGGAGTTTAGGCTTGGTGGTACTCGAGTGTGCCATCGGTAGGTTTCCTTACATGCAATCTGAAGATCAACAAAGCTGGCCAAGCTTTTATGAGCTTTTGGAGGCAATTGTGGAAAGTCCACCACCTTCAGCTCCTCCGGATCAGTTCTCCCCCGAGTTCTGTTCTTTCGTGTCTTCCTGCATACAGAAGGACCCTCAACGCAGATCATCATCTTTGGACCTTTTGGGTCACCCCTTCATCAAGAAATTCGAAAACAAAGACATTGATCTCGGGATTCTGGTTGGCAGCTTAGAACCTCCTGTAAATTTTCCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009653 GO:0009791 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010200 GO:0010243 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0033206 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04010 ko04012 ko04013 ko04014 ko04015 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04140 ko04150 ko04151 ko04210 ko04218 ko04270 ko04320 ko04370 ko04371 ko04380 ko04510 ko04540 ko04550 ko04620 ko04626 ko04650 ko04660 ko04662 ko04664 ko04666 ko04668 ko04720 ko04722 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04934 ko05020 ko05034 ko05161 ko05164 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04010 map04012 map04013 map04014 map04015 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04140 map04150 map04151 map04210 map04218 map04270 map04320 map04370 map04371 map04380 map04510 map04540 map04550 map04620 map04626 map04650 map04660 map04662 map04664 map04666 map04668 map04720 map04722 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04934 map05020 map05034 map05161 map05164 map05165 map05167 map05200 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
Pkinase

Protein Analysis

361

Amino Acids

40.48

Weight (kDa)

6.14

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 76 - 335 2.1e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 76 - 326 1.9e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56580
fragaria_vesca FvH4_1g06420 FvH4_1g06420
malus_domestica MD02G1067500.v1.1 MD15G1199300.v1.1
prunus_persica Prupe.7G217900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0092161
rosa_laevigata RLG00000016280
rosa_multiflora Rmu_co8378983.1_g000001 Rmu_sc0000524.1_g000012
rosa_roxburghii Rroxscaffold_2G00149420
rosa_rugosa Rorug02G0023200 Rorug02G0023300
rosa_samantha Rh2AG069000 Rh2BG068300 Rh2CG069500 Rh2DG067500
rosa_wichuraiana Rw2G006330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 619
Acc36I ACCTGC 1 cut(s) 619
AccIII TCCGGA 1 cut(s) 901
AciI CCGC 2 cut(s) 142, 735
AclWI GGATC 1 cut(s) 912
AcoI YGGCCR 1 cut(s) 842
AcsI RAATTY 4 cut(s) 22, 339, 1010, 1069
AcuI CTGAAG 3 cut(s) 174, 846, 876
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 5 cut(s) 267, 557, 703, 742, 786
AfiI CCNNNNNNNGG 1 cut(s) 804
AflIII ACRYGT 3 cut(s) 394, 574, 714
AgsI TTSAA 6 cut(s) 22, 28, 34, 206, 310, 509
AhdI GACNNNNNGTC 1 cut(s) 466
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 5 cut(s) 158, 221, 474, 582, 681
AlwI GGATC 1 cut(s) 912
Ama87I CYCGRG 3 cut(s) 788, 917, 1034
Aor13HI TCCGGA 1 cut(s) 901
AoxI GGCC 2 cut(s) 432, 842
ApeKI GCWGC 3 cut(s) 37, 380, 1050
ApoI RAATTY 4 cut(s) 22, 339, 1010, 1069
AspS9I GGNCC 4 cut(s) 432, 460, 955, 982
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 5 cut(s) 325, 401, 631, 643, 986
AsuII TTCGAA 1 cut(s) 1014
AsuNHI GCTAGC 2 cut(s) 664, 668
AvaI CYCGRG 3 cut(s) 788, 917, 1034
AvaII GGWCC 3 cut(s) 460, 955, 982
BaeI ACNNNNGTAYC 2 cut(s) 776, 809
BalI TGGCCA 1 cut(s) 844
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 3 cut(s) 11, 17, 930
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 49, 392, 1062
BccI CCATC 3 cut(s) 607, 627, 806
BceAI ACGGC 1 cut(s) 127
BclI TGATCA 1 cut(s) 483
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 5 cut(s) 158, 221, 474, 582, 681
BfaI CTAG 4 cut(s) 551, 611, 665, 669
BfmI CTRYAG 1 cut(s) 751
BfuAI ACCTGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 220
BisI GCNGC 4 cut(s) 38, 143, 381, 1051
BlsI GCNGC 4 cut(s) 39, 144, 382, 1052
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 3 cut(s) 460, 955, 982
BmeRI GACNNNNNGTC 1 cut(s) 466
BmeT110I CYCGRG 3 cut(s) 788, 917, 1034
BmgT120I GGNCC 4 cut(s) 432, 460, 955, 982
BmiI GGNNCC 2 cut(s) 433, 957
BmrFI CCNGG 1 cut(s) 464
BmtI GCTAGC 2 cut(s) 668, 672
BpiI GAAGAC 3 cut(s) 11, 17, 930
Bpu14I TTCGAA 1 cut(s) 1014
BpuEI CTTGAG 3 cut(s) 26, 45, 245
BpuMI CCSGG 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 973
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 158, 474
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 1 cut(s) 901
Bsc4I CCNNNNNNNGG 1 cut(s) 804
Bse1I ACTGG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 973
BseAI TCCGGA 1 cut(s) 901
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 1 cut(s) 599
BseJI GATNNNNATC 1 cut(s) 973
BseLI CCNNNNNNNGG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 888
BseXI GCAGC 3 cut(s) 49, 392, 1062
BsgI GTGCAG 1 cut(s) 377
BshFI GGCC 2 cut(s) 434, 844
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 3 cut(s) 788, 917, 1034
BsiSI CCGG 3 cut(s) 463, 722, 902
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 804
BsmAI GTCTC 5 cut(s) 158, 221, 474, 582, 681
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 434, 844
Bso31I GGTCTC 2 cut(s) 158, 474
BsoBI CYCGRG 3 cut(s) 788, 917, 1034
Bsp119I TTCGAA 1 cut(s) 1014
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 901
BspACI CCGC 2 cut(s) 142, 735
BspANI GGCC 2 cut(s) 434, 844
BspEI TCCGGA 1 cut(s) 901
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 433, 957
BspMI ACCTGC 1 cut(s) 619
BspOI GCTAGC 2 cut(s) 668, 672
BspPI GGATC 1 cut(s) 912
BspT104I TTCGAA 1 cut(s) 1014
BspTNI GGTCTC 2 cut(s) 158, 474
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 1014
BstC8I GCNNGC 3 cut(s) 666, 670, 842
BstDEI CTNAG 1 cut(s) 1054
BstEII GGTNACC 1 cut(s) 992
BstF5I GGATG 1 cut(s) 599
BstMAI GTCTC 5 cut(s) 158, 221, 474, 582, 681
BstMWI GCNNNNNNNGC 4 cut(s) 43, 65, 665, 846
BstNSI RCATGY 4 cut(s) 398, 578, 718, 820
BstPI GGTNACC 1 cut(s) 992
BstSCI CCNGG 1 cut(s) 462
BstSFI CTRYAG 1 cut(s) 751
BstV1I GCAGC 3 cut(s) 49, 392, 1062
BstV2I GAAGAC 3 cut(s) 11, 17, 930
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 2 cut(s) 434, 844
BtsCI GGATG 1 cut(s) 599
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 2 cut(s) 100, 763
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 3 cut(s) 666, 670, 842
CciI TCATGA 1 cut(s) 106
Cfr13I GGNCC 4 cut(s) 432, 460, 955, 982
CseI GACGC 2 cut(s) 22, 110
Csp6I GTAC 5 cut(s) 266, 556, 702, 741, 785
CviAII CATG 5 cut(s) 107, 395, 575, 715, 817
CviQI GTAC 5 cut(s) 266, 556, 702, 741, 785
DdeI CTNAG 1 cut(s) 1054
DriI GACNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 842
Eam1105I GACNNNNNGTC 1 cut(s) 466
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 2 cut(s) 158, 474
Eco47I GGWCC 3 cut(s) 460, 955, 982
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 3 cut(s) 174, 846, 876
Eco88I CYCGRG 3 cut(s) 788, 917, 1034
Eco91I GGTNACC 1 cut(s) 992
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 955
EcoO65I GGTNACC 1 cut(s) 992
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 5 cut(s) 110, 398, 578, 718, 820
FaqI GGGAC 1 cut(s) 38
FatI CATG 5 cut(s) 106, 394, 574, 714, 816
FauI CCCGC 1 cut(s) 728
FbaI TGATCA 1 cut(s) 483
Fnu4HI GCNGC 4 cut(s) 38, 143, 381, 1051
FokI GGATG 1 cut(s) 586
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 4 cut(s) 38, 143, 381, 1051
FspBI CTAG 4 cut(s) 551, 611, 665, 669
GluI GCNGC 4 cut(s) 38, 143, 381, 1051
HaeIII GGCC 2 cut(s) 434, 844
HapII CCGG 3 cut(s) 463, 722, 902
HgaI GACGC 2 cut(s) 22, 110
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 5 cut(s) 110, 398, 578, 718, 820
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 1 cut(s) 847
HinfI GANTC 2 cut(s) 316, 1039
HpaI GTTAAC 1 cut(s) 496
HpaII CCGG 3 cut(s) 463, 722, 902
HphI GGTGA 5 cut(s) 325, 401, 631, 643, 986
Hpy166II GTNNAC 7 cut(s) 347, 392, 496, 533, 556, 616, 884
Hpy188I TCNGA 8 cut(s) 87, 126, 154, 184, 293, 321, 488, 826
Hpy188III TCNNGA 8 cut(s) 62, 107, 224, 244, 506, 902, 1006, 1036
Hpy8I GTNNAC 7 cut(s) 347, 392, 496, 533, 556, 616, 884
HpyAV CCTTC 5 cut(s) 241, 304, 900, 946, 1009
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4V TGCA 6 cut(s) 358, 476, 521, 562, 820, 945
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 65, 665, 846
HpyF3I CTNAG 1 cut(s) 1054
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 5 cut(s) 110, 398, 578, 718, 820
Kpn2I TCCGGA 1 cut(s) 901
Ksp22I TGATCA 1 cut(s) 483
KspAI GTTAAC 1 cut(s) 496
LmnI GCTCC 3 cut(s) 193, 361, 901
Lsp1109I GCAGC 3 cut(s) 49, 392, 1062
MaeI CTAG 4 cut(s) 551, 611, 665, 669
MaeIII GTNAC 2 cut(s) 407, 992
MboII GAAGA 7 cut(s) 16, 22, 167, 230, 646, 839, 930
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 844
MluCI AATT 8 cut(s) 22, 207, 339, 501, 709, 870, 1010, 1069
MluNI TGGCCA 1 cut(s) 844
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 6 cut(s) 328, 720, 859, 909, 969, 1071
Mox20I TGGCCA 1 cut(s) 844
MroI TCCGGA 1 cut(s) 901
MscI TGGCCA 1 cut(s) 844
MseI TTAA 1 cut(s) 495
Msp20I TGGCCA 1 cut(s) 844
MspI CCGG 3 cut(s) 463, 722, 902
MspR9I CCNGG 1 cut(s) 464
MunI CAATTG 1 cut(s) 870
MwoI GCNNNNNNNGC 4 cut(s) 43, 65, 665, 846
NciI CCSGG 1 cut(s) 464
NheI GCTAGC 2 cut(s) 664, 668
NlaIII CATG 5 cut(s) 110, 398, 578, 718, 820
NlaIV GGNNCC 2 cut(s) 433, 957
NmuCI GTSAC 2 cut(s) 407, 992
NspI RCATGY 4 cut(s) 398, 578, 718, 820
NspV TTCGAA 1 cut(s) 1014
PaeR7I CTCGAG 1 cut(s) 788
PagI TCATGA 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 619
PciI ACATGT 3 cut(s) 394, 574, 714
PfeI GAWTC 2 cut(s) 316, 1039
PkrI GCNGC 4 cut(s) 39, 144, 382, 1052
PpuMI RGGWCCY 1 cut(s) 955
PscI ACATGT 3 cut(s) 394, 574, 714
Psp124BI GAGCTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 955
PspEI GGTNACC 1 cut(s) 992
PspN4I GGNNCC 2 cut(s) 433, 957
PspPI GGNCC 4 cut(s) 432, 460, 955, 982
PspPPI RGGWCCY 1 cut(s) 955
PspXI VCTCGAGB 1 cut(s) 788
PsrI GAACNNNNNNTAC 2 cut(s) 258, 290
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 5 cut(s) 267, 557, 703, 742, 786
RsaNI GTAC 5 cut(s) 266, 556, 702, 741, 785
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 4 cut(s) 38, 143, 381, 1051
Sau96I GGNCC 4 cut(s) 432, 460, 955, 982
ScaI AGTACT 1 cut(s) 742
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 198
SfcI CTRYAG 1 cut(s) 751
Sfr274I CTCGAG 1 cut(s) 788
SfuI TTCGAA 1 cut(s) 1014
SinI GGWCC 3 cut(s) 460, 955, 982
SlaI CTCGAG 1 cut(s) 788
SmlI CTYRAG 4 cut(s) 41, 60, 224, 788
SmoI CTYRAG 4 cut(s) 41, 60, 224, 788
Sse9I AATT 8 cut(s) 22, 207, 339, 501, 709, 870, 1010, 1069
SsiI CCGC 2 cut(s) 142, 735
SspMI CTAG 4 cut(s) 551, 611, 665, 669
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 462
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 2 cut(s) 789, 1014
TasI AATT 8 cut(s) 22, 207, 339, 501, 709, 870, 1010, 1069
TatI WGTACW 3 cut(s) 265, 555, 740
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 316, 1039
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 2 cut(s) 100, 763
TseFI GTSAC 2 cut(s) 407, 992
TseI GCWGC 3 cut(s) 37, 380, 1050
Tsp45I GTSAC 2 cut(s) 407, 992
TspDTI ATGAA 5 cut(s) 17, 95, 338, 582, 991
TspRI CASTG 2 cut(s) 100, 763
VpaK11BI GGWCC 3 cut(s) 460, 955, 982
XapI RAATTY 4 cut(s) 22, 339, 1010, 1069
XceI RCATGY 4 cut(s) 398, 578, 718, 820
XhoI CTCGAG 1 cut(s) 788
XspI CTAG 4 cut(s) 551, 611, 665, 669
ZrmI AGTACT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.