Rh2BG068300

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012782
Physical Location & Seq
Reverse (-)
5175973 .. 5179469
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG068300.1

Sequence Viewer

Length: 1218 bp
ATGAAGACGAAGACACCATTGAATCTGAAGAAGCAGCTCAAGCTCTCTGTTCCTGCTCAAGAAACCAACATCACCTCATTCTTGACTGCCAGTGGCACATTTCATGATGGGGGTTTGCTTCTGAACCAGAAAGGGTTGCGGCTTATCTCCGAAGAAAAGGAGGTCCGACCTTCTGATAGCAAGGAGCTCAATGTTGAATTTTCCTTGGAAGATCTCGAGACTATCAAAGTGATTGGAAAGGGAAGTGGTGGTGTAGTTCAACTTGTTCGCCATAAATGGGTGGGAAAACTATTTGCTTTGAAGGTTATTCAGATGAACATACAAGAGGAAATTCGTAAACAGATTGTGCAGGAGCTAAAAATAAACCAAGCAGCACAATGTCCACATGTGGTAGTTTGCCACCACTCCTTTTATCACAATGGAGCAATTTCTCTTGTGTTAGAATACATGGATCGTGGATCTCTTGCAGATGTGATCAGACAAGTTAAGACAATTCTTGAACCATATCTTGCAGTCGTTTGTAAACAGGTTTTACAAGGTCTTGTCTACTTGCACAATGAAAGGCATGTAATACATAGAGACATAAAACCATCCAATCTGCTAGTAAACCACAAGGGGGAGGTCAAGATTACTGATTTTGGTGTGAGTGCTTCACTTGCTAGCTCCATGGGTCAGAGGGACACATTCGTTGGTACTTACAATTATATGTCGGTATGTAATGATCCTTTTCATTTTCGCTTCCTTTCACTGCTAGCTCCCAAGCTTAAAATTACAGTCTTTCTGTTCAAGCTTGGCACATACCAGAGATACAATGCAGAATTTGAGTTACTAAACAATGTGATTGATTTCTTTCAGCCAGAGAGAATTAAAGGGGGTACTTATGACTACAGCAGTGACATTTGGAGTTTAGGCATGGTGATACTTGAGTGTGCTATTGGACGGTTTCCTTACATGCAATCTGAAGATGAACAAAGCTGGCCAAGCTTTTATGAGCTTTTGGAGGCAATTGTTGGAAGTCCACCACCTACTGCTCCATCGGATCAGTTCTCTCCCGAGTTCTGTTCTTTTGTGTCTGCCTGCATACAGAAAGACCCTCAACACAGATCATCATCTTTGGATCTTTTGAGTCACCCATTCATCAAAAAGTTTGAAGACAAGGACATTGATCTTGAGATTCTGGTGGGTAGCTTGGAATCCCCTGTAAATTTTCCCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000187 GO:0000280 GO:0000910 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007346 GO:0008150 GO:0008152 GO:0009524 GO:0009653 GO:0009791 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010200 GO:0010243 GO:0010311 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0033206 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045859 GO:0045860 GO:0045937 GO:0048232 GO:0048285 GO:0048364 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051321 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061640 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1902531 GO:1902533 GO:1903046 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04010 ko04012 ko04013 ko04014 ko04015 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04140 ko04150 ko04151 ko04210 ko04218 ko04270 ko04320 ko04370 ko04371 ko04380 ko04510 ko04540 ko04550 ko04620 ko04626 ko04650 ko04660 ko04662 ko04664 ko04666 ko04668 ko04720 ko04722 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04934 ko05020 ko05034 ko05161 ko05164 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04010 map04012 map04013 map04014 map04015 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04140 map04150 map04151 map04210 map04218 map04270 map04320 map04370 map04371 map04380 map04510 map04540 map04550 map04620 map04626 map04650 map04660 map04662 map04664 map04666 map04668 map04720 map04722 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04934 map05020 map05034 map05161 map05164 map05165 map05167 map05200 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
Pkinase

Protein Analysis

405

Amino Acids

45.84

Weight (kDa)

6.51

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 73 - 237 4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 73 - 221 1.3e-23 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 279 - 373 4.7e-06 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 282 - 380 1.5e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56580
fragaria_vesca FvH4_1g06420 FvH4_1g06420
malus_domestica MD02G1067500.v1.1 MD15G1199300.v1.1
prunus_persica Prupe.7G217900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0092161
rosa_laevigata RLG00000016280
rosa_multiflora Rmu_co8378983.1_g000001 Rmu_sc0000524.1_g000012
rosa_roxburghii Rroxscaffold_2G00149420
rosa_rugosa Rorug02G0023200 Rorug02G0023300
rosa_samantha Rh2AG069000 Rh2BG068300 Rh2CG069500 Rh2DG067500
rosa_wichuraiana Rw2G006330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 546
AciI CCGC 1 cut(s) 139
AclWI GGATC 5 cut(s) 459, 466, 716, 1047, 1125
AcoI YGGCCR 1 cut(s) 977
AcsI RAATTY 4 cut(s) 197, 330, 818, 1204
AcuI CTGAAG 2 cut(s) 47, 981
AfaI GTAC 2 cut(s) 694, 877
AfiI CCNNNNNNNGG 2 cut(s) 277, 616
AflIII ACRYGT 1 cut(s) 385
AgsI TTSAA 7 cut(s) 22, 197, 260, 301, 500, 787, 1151
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 212, 573
AlwI GGATC 5 cut(s) 459, 466, 716, 1047, 1125
Ama87I CYCGRG 2 cut(s) 215, 1052
AoxI GGCC 1 cut(s) 977
ApeKI GCWGC 2 cut(s) 34, 371
ApoI RAATTY 4 cut(s) 197, 330, 818, 1204
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 64, 928, 1121
AsuNHI GCTAGC 2 cut(s) 659, 751
AvaI CYCGRG 2 cut(s) 215, 1052
AvaII GGWCC 1 cut(s) 163
BaeI ACNNNNGTAYC 2 cut(s) 911, 944
BalI TGGCCA 1 cut(s) 979
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 3 cut(s) 11, 17, 1158
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 46, 383
BccI CCATC 3 cut(s) 101, 598, 1042
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 212, 573
BfaI CTAG 3 cut(s) 602, 660, 752
BfmI CTRYAG 1 cut(s) 886
BglII AGATCT 1 cut(s) 211
BisI GCNGC 3 cut(s) 35, 140, 372
BlsI GCNGC 3 cut(s) 36, 141, 373
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 2 cut(s) 215, 1052
BmgT120I GGNCC 1 cut(s) 163
BmtI GCTAGC 2 cut(s) 663, 755
BpiI GAAGAC 3 cut(s) 11, 17, 1158
BpuEI CTTGAG 4 cut(s) 23, 42, 944, 1190
BsaBI GATNNNNATC 1 cut(s) 1108
BsaJI CCNNGG 2 cut(s) 204, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 616
Bse1I ACTGG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 1108
BseDI CCNNGG 2 cut(s) 204, 666
BseGI GGATG 1 cut(s) 590
BseJI GATNNNNATC 1 cut(s) 1108
BseLI CCNNNNNNNGG 2 cut(s) 277, 616
BseNI ACTGG 1 cut(s) 90
BseXI GCAGC 2 cut(s) 46, 383
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 2 cut(s) 215, 1052
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 2 cut(s) 277, 616
BsmAI GTCTC 2 cut(s) 212, 573
BsmFI GGGAC 1 cut(s) 692
BsnI GGCC 1 cut(s) 979
BsoBI CYCGRG 2 cut(s) 215, 1052
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 1 cut(s) 139
BspANI GGCC 1 cut(s) 979
BspHI TCATGA 1 cut(s) 103
BspOI GCTAGC 2 cut(s) 663, 755
BspPI GGATC 5 cut(s) 459, 466, 716, 1047, 1125
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 2 cut(s) 204, 666
BssMI GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
BssT1I CCWWGG 2 cut(s) 204, 666
Bst4CI ACNGT 2 cut(s) 775, 942
BstC8I GCNNGC 4 cut(s) 661, 753, 977, 1078
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 1 cut(s) 590
BstKTI GATC 9 cut(s) 214, 454, 461, 477, 724, 1042, 1106, 1120, 1168
BstMAI GTCTC 2 cut(s) 212, 573
BstMBI GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
BstMWI GCNNNNNNNGC 3 cut(s) 40, 656, 981
BstNSI RCATGY 3 cut(s) 389, 569, 955
BstSFI CTRYAG 1 cut(s) 886
BstV1I GCAGC 2 cut(s) 46, 383
BstV2I GAAGAC 3 cut(s) 11, 17, 1158
BstX2I RGATCY 3 cut(s) 211, 458, 1117
BstYI RGATCY 3 cut(s) 211, 458, 1117
BsuRI GGCC 1 cut(s) 979
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 590
BtsI GCAGTG 2 cut(s) 746, 898
BtsIMutI CAGTG 3 cut(s) 97, 746, 898
Cac8I GCNNGC 4 cut(s) 661, 753, 977, 1078
CciI TCATGA 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 693, 876
CviAII CATG 7 cut(s) 104, 386, 448, 566, 667, 913, 952
CviQI GTAC 2 cut(s) 693, 876
DpnI GATC 9 cut(s) 213, 453, 460, 476, 723, 1041, 1105, 1119, 1167
DpnII GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
EaeI YGGCCR 1 cut(s) 977
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 204, 666
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 47, 981
Eco88I CYCGRG 2 cut(s) 215, 1052
EcoICRI GAGCTC 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 204, 666
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 2 cut(s) 204, 666
FaeI CATG 7 cut(s) 107, 389, 451, 569, 670, 916, 955
FaqI GGGAC 1 cut(s) 692
FatI CATG 7 cut(s) 103, 385, 447, 565, 666, 912, 951
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 3 cut(s) 35, 140, 372
FokI GGATG 1 cut(s) 577
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 3 cut(s) 35, 140, 372
FspBI CTAG 3 cut(s) 602, 660, 752
GluI GCNGC 3 cut(s) 35, 140, 372
HaeIII GGCC 1 cut(s) 979
Hin1II CATG 7 cut(s) 107, 389, 451, 569, 670, 916, 955
HindIII AAGCTT 3 cut(s) 761, 788, 982
HinfI GANTC 4 cut(s) 22, 1126, 1174, 1193
HphI GGTGA 3 cut(s) 64, 928, 1121
Hpy166II GTNNAC 6 cut(s) 338, 383, 524, 547, 607, 1019
Hpy188III TCNNGA 9 cut(s) 59, 82, 104, 215, 217, 497, 625, 1052, 1169
Hpy8I GTNNAC 6 cut(s) 338, 383, 524, 547, 607, 1019
HpyAV CCTTC 2 cut(s) 180, 295
HpyCH4III ACNGT 2 cut(s) 775, 942
HpyCH4V TGCA 7 cut(s) 349, 467, 512, 553, 815, 955, 1080
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 656, 981
Hsp92II CATG 7 cut(s) 107, 389, 451, 569, 670, 916, 955
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
LmnI GCTCC 6 cut(s) 184, 352, 422, 668, 760, 1036
Lsp1109I GCAGC 2 cut(s) 46, 383
MaeI CTAG 3 cut(s) 602, 660, 752
MaeIII GTNAC 3 cut(s) 825, 893, 1127
MalI GATC 9 cut(s) 213, 453, 460, 476, 723, 1041, 1105, 1119, 1167
MboI GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
MboII GAAGA 7 cut(s) 16, 22, 40, 164, 221, 974, 1163
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 3 cut(s) 211, 458, 1117
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 979
MluNI TGGCCA 1 cut(s) 979
MlyI GAGTC 1 cut(s) 1135
MmeI TCCRAC 2 cut(s) 190, 991
MnlI CCTC 7 cut(s) 85, 154, 319, 613, 669, 994, 1104
Mox20I TGGCCA 1 cut(s) 979
MscI TGGCCA 1 cut(s) 979
MseI TTAA 3 cut(s) 486, 765, 867
Msp20I TGGCCA 1 cut(s) 979
MunI CAATTG 1 cut(s) 1005
MwoI GCNNNNNNNGC 3 cut(s) 40, 656, 981
NcoI CCATGG 1 cut(s) 666
NdeII GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
NheI GCTAGC 2 cut(s) 659, 751
NlaIII CATG 7 cut(s) 107, 389, 451, 569, 670, 916, 955
NmuCI GTSAC 2 cut(s) 893, 1127
NspI RCATGY 3 cut(s) 389, 569, 955
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 103
PciI ACATGT 1 cut(s) 385
PfeI GAWTC 3 cut(s) 22, 1174, 1193
PkrI GCNGC 3 cut(s) 36, 141, 373
PleI GAGTC 1 cut(s) 1134
PpsI GAGTC 1 cut(s) 1134
PscI ACATGT 1 cut(s) 385
Psp124BI GAGCTC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 163
PsuI RGATCY 3 cut(s) 211, 458, 1117
RsaI GTAC 2 cut(s) 694, 877
RsaNI GTAC 2 cut(s) 693, 876
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 3 cut(s) 486, 765, 867
SatI GCNGC 3 cut(s) 35, 140, 372
Sau3AI GATC 9 cut(s) 211, 451, 458, 474, 721, 1039, 1103, 1117, 1165
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 1135
SduI GDGCHC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 886
Sfr274I CTCGAG 1 cut(s) 215
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 5 cut(s) 38, 57, 215, 923, 1169
SmoI CTYRAG 5 cut(s) 38, 57, 215, 923, 1169
SsiI CCGC 1 cut(s) 139
SspMI CTAG 3 cut(s) 602, 660, 752
SstI GAGCTC 1 cut(s) 189
StyI CCWWGG 2 cut(s) 204, 666
TaaI ACNGT 2 cut(s) 775, 942
TaqI TCGA 1 cut(s) 216
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 3 cut(s) 22, 1174, 1193
Tru1I TTAA 3 cut(s) 486, 765, 867
Tru9I TTAA 3 cut(s) 486, 765, 867
TscAI CASTG 3 cut(s) 97, 753, 898
TseFI GTSAC 2 cut(s) 893, 1127
TseI GCWGC 2 cut(s) 34, 371
Tsp45I GTSAC 2 cut(s) 893, 1127
TspDTI ATGAA 7 cut(s) 17, 92, 329, 573, 719, 981, 1126
TspRI CASTG 3 cut(s) 97, 753, 898
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 4 cut(s) 197, 330, 818, 1204
XceI RCATGY 3 cut(s) 389, 569, 955
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 546
XspI CTAG 3 cut(s) 602, 660, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.