MD02G1113200.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0021169
Physical Location & Seq
Forward (+)
9190859 .. 9191712
854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1113200.v1.1.491

Sequence Viewer

Length: 525 bp
ATGCATGACTTACTAGAAAAAATGGGTAAGCGTATAGTTAGTGAAGAATCACCCATTGAACCAGGCAAGCGGAGCAGATTGTGGCATCATGAAGATGTGTACCGTGTTCTAACTGAAAACAGAGGAACCAAGAAAATTAAAGGCATTGTGGTAAAGTGGCCCAAACCAGATTTGATACCTTTGAATTCCAAAAGCTTCTTCAAGATGGTAAATCTTGAAATTTTTATAAGCCGTAATGCACTTTTTTCTGGATGCGTTGATTATATGCCCAACTATTTAAGGTGGATTGATTTGGGTGGAGATAAGTTTCGAATTTGGGGATCCAATATTCTTCAAAAGCATACGGTTACTTTCAATTTGCAATATCTTCCAAGACATCTTGTGACATATAATATGTCATACAAATTTGGCAAATCTTACATATATGAATTTACGTGGGTGCAAATTTCTGGAAAAAATCCCCGACTTATCCGGAAGTCCAAACATAGAATTTTTGGATCTTCATGGCTGCACAAATTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

175

Amino Acids

20.69

Weight (kDa)

10.06

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021169)

Species Orthologous Gene IDs
malus_domestica MD02G1113200.v1.1
rosa_laevigata RLG00000029814
rosa_roxburghii Rroxscaffold_4G00319990 Rroxscaffold_4G00320110
rosa_samantha Rh1BG084700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AccIII TCCGGA 1 cut(s) 471
AciI CCGC 1 cut(s) 70
AclWI GGATC 3 cut(s) 315, 328, 505
AcsI RAATTY 8 cut(s) 184, 219, 312, 404, 428, 444, 489, 515
AfaI GTAC 1 cut(s) 101
AgsI TTSAA 6 cut(s) 59, 184, 202, 218, 335, 355
AjnI CCWGG 1 cut(s) 61
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 1 cut(s) 195
AluI AGCT 1 cut(s) 195
AlwI GGATC 3 cut(s) 315, 328, 505
Aor13HI TCCGGA 1 cut(s) 471
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 8 cut(s) 184, 219, 312, 404, 428, 444, 489, 515
AspS9I GGNCC 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 42
AsuII TTCGAA 1 cut(s) 310
BamHI GGATCC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 495
BccI CCATC 1 cut(s) 199
BceAI ACGGC 1 cut(s) 216
BciT130I CCWGG 1 cut(s) 63
BfaI CTAG 1 cut(s) 14
BisI GCNGC 1 cut(s) 509
BlsI GCNGC 1 cut(s) 510
Bme1390I CCNGG 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 127, 322
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 2 cut(s) 94, 242
Bpu14I TTCGAA 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 471
BseBI CCWGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 257
BseXI GCAGC 1 cut(s) 495
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 1 cut(s) 160
BsiSI CCGG 1 cut(s) 472
BsnI GGCC 1 cut(s) 160
Bsp119I TTCGAA 1 cut(s) 310
Bsp13I TCCGGA 1 cut(s) 471
Bsp143I GATC 2 cut(s) 320, 497
BspACI CCGC 1 cut(s) 70
BspANI GGCC 1 cut(s) 160
BspEI TCCGGA 1 cut(s) 471
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 127, 322
BspPI GGATC 3 cut(s) 315, 328, 505
BspT104I TTCGAA 1 cut(s) 310
BssMI GATC 2 cut(s) 320, 497
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 104, 346
BstBAI YACGTR 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 310
BstC8I GCNNGC 1 cut(s) 68
BstF5I GGATG 1 cut(s) 257
BstKTI GATC 2 cut(s) 323, 500
BstMBI GATC 2 cut(s) 320, 497
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 495
BstX2I RGATCY 2 cut(s) 320, 497
BstYI RGATCY 2 cut(s) 320, 497
BsuRI GGCC 1 cut(s) 160
BtsCI GGATG 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 68
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 5, 89, 504
CviJI RGCY 4 cut(s) 160, 195, 231, 508
CviKI_1 RGCY 4 cut(s) 160, 195, 231, 508
CviQI GTAC 1 cut(s) 100
DpnI GATC 2 cut(s) 322, 499
DpnII GATC 2 cut(s) 320, 497
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 61
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 8, 92, 507
FatI CATG 3 cut(s) 4, 88, 503
Fnu4HI GCNGC 1 cut(s) 509
FokI GGATG 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 509
FspBI CTAG 1 cut(s) 14
GluI GCNGC 1 cut(s) 509
HaeIII GGCC 1 cut(s) 160
HapII CCGG 1 cut(s) 472
Hin1II CATG 3 cut(s) 8, 92, 507
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 1 cut(s) 47
HpaII CCGG 1 cut(s) 472
HphI GGTGA 1 cut(s) 42
Hpy166II GTNNAC 1 cut(s) 100
Hpy188III TCNNGA 6 cut(s) 89, 202, 215, 249, 450, 472
Hpy8I GTNNAC 1 cut(s) 100
HpyCH4III ACNGT 2 cut(s) 104, 346
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 5 cut(s) 4, 239, 361, 442, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 3 cut(s) 8, 92, 507
Kpn2I TCCGGA 1 cut(s) 471
Kzo9I GATC 2 cut(s) 320, 497
LmnI GCTCC 1 cut(s) 72
LpnPI CCDG 6 cut(s) 48, 75, 180, 234, 435, 485
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 2 cut(s) 94, 242
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 2 cut(s) 346, 382
MalI GATC 2 cut(s) 322, 499
MboI GATC 2 cut(s) 320, 497
MboII GAAGA 6 cut(s) 56, 104, 190, 323, 359, 492
MflI RGATCY 2 cut(s) 320, 497
MnlI CCTC 1 cut(s) 116
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 471
MseI TTAA 2 cut(s) 138, 278
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 1 cut(s) 72
NdeII GATC 2 cut(s) 320, 497
NlaIII CATG 3 cut(s) 8, 92, 507
NlaIV GGNNCC 2 cut(s) 127, 322
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 310
PagI TCATGA 1 cut(s) 88
PfeI GAWTC 1 cut(s) 47
PkrI GCNGC 1 cut(s) 510
Ppu21I YACGTR 1 cut(s) 435
PsiI TTATAA 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 2 cut(s) 127, 322
PspPI GGNCC 1 cut(s) 159
PsuI RGATCY 2 cut(s) 320, 497
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 2 cut(s) 138, 278
SatI GCNGC 1 cut(s) 509
Sau3AI GATC 2 cut(s) 320, 497
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 1 cut(s) 63
SetI ASST 4 cut(s) 181, 197, 284, 437
SfaNI GCATC 2 cut(s) 94, 242
SfuI TTCGAA 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 93
SsiI CCGC 1 cut(s) 70
SspI AATATT 1 cut(s) 328
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 61
TaaI ACNGT 2 cut(s) 104, 346
TaiI ACGT 1 cut(s) 437
TaqI TCGA 1 cut(s) 310
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 2 cut(s) 138, 278
Tru9I TTAA 2 cut(s) 138, 278
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 1 cut(s) 508
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 3 cut(s) 105, 441, 492
XapI RAATTY 8 cut(s) 184, 219, 312, 404, 428, 444, 489, 515
XspI CTAG 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.