Rroxscaffold_4G00320110

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0021169
Physical Location & Seq
Forward (+)
48497935 .. 48500359
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320110.1

Sequence Viewer

Length: 1089 bp
ATGCATGACTTATTAGAACAGATGGGTAAGGATATTGTTCATCAAGAATCTCCTACGGATCCTGGGAGACGTAGCCGATTGTGGTTTCACGACGATGTCAACCGGGTTTTAACAGAAAACACAGGAAATGAAAAAGTTAAAGGCATAATCTTAAAATGGCTCCCCGGACCATATAAGATACAGTTGAATGCAAAATGCTTCTCAAAGATGAAGAATCTTGAACTTCTTATAAGCGGTAATGCATGCCTTTCTAGAGAAGTGGATTATCTCCCCAACAGATTGAGGTTGCTTGATTGGGATGAATGTGAGTTACAATCTTTGCCATCCAATTTTCATCCAAAGGAACTTGTTGTTTTCAAAATGCCTCGCGGTCTAATCACCCAATTAGGACAAGGATTTCAGCTGGCTTGTTGTCGTGATGAGGACTTTGCAATTGTACTTCCAGGCAATCAAGTTCCAAAGTGGTTCAAGTATCGTGAGCATCCAAATTACATATATATACCTTCAACTTTAGAGGAAGATGAAGAGAAGCTTGATATATGTGAAGTTTCTATTGGAGTTCCTCCAAATTCGGTGAAGGTTGGATTGGCTCTCTATGCTGTTTTTGAAGTTACCCAAAATAATGGTGGTCTTTTTTCTATGGATGTCGATATTATGCATAATGGGAGATTCTTGGGTTATAGGACATGTATTGACATGACTAATATAGAGACAGAGTCAACCCATGTGTCCCTGAATTATTTCAATGTGTCCTTAAATGGAGCCGAAAGCAATGGTACATGTCAATTGATATTATTTTGGCATCATAATGGTGATGCCGGATTGTCGTTGAAAAGCTGCGGATTTCACATTGTCGATAATTGTGGCGTTATGACTCTTGATAATAATGATTTAGATCAAGAAAAGGAAGAGCAAGAGCAAGAGCAGTGGCTTTCTTCATCGCTAGCAAAGAGACCTCGCGGAGTAGCAGGGGAAGATAATCAAGATCTGCACGATCGGTGTTGTACGTCTTCGACTTCATCGAATCAATTGCAAACAAGCGGAACCGTGTTGAGGAGGAGCAAACTCATCACTTCATTTGAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

362

Amino Acids

41.03

Weight (kDa)

5.2

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021169)

Species Orthologous Gene IDs
malus_domestica MD02G1113200.v1.1
rosa_laevigata RLG00000029814
rosa_roxburghii Rroxscaffold_4G00319990 Rroxscaffold_4G00320110
rosa_samantha Rh1BG084700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 230
AccII CGCG 2 cut(s) 369, 960
AciI CCGC 5 cut(s) 234, 369, 840, 960, 1041
AclWI GGATC 2 cut(s) 53, 66
AcsI RAATTY 1 cut(s) 568
AfaI GTAC 3 cut(s) 438, 778, 1006
AfiI CCNNNNNNNGG 1 cut(s) 1053
AflIII ACRYGT 2 cut(s) 686, 779
AgsI TTSAA 8 cut(s) 187, 221, 358, 469, 507, 608, 745, 832
AjnI CCWGG 2 cut(s) 61, 442
AjuI GAANNNNNNNTTGG 4 cut(s) 537, 569, 569, 601
AluBI AGCT 3 cut(s) 403, 532, 837
AluI AGCT 3 cut(s) 403, 532, 837
Alw26I GTCTC 3 cut(s) 61, 704, 946
AlwI GGATC 2 cut(s) 53, 66
ApeKI GCWGC 1 cut(s) 837
ApoI RAATTY 1 cut(s) 568
Asp700I GAANNNNTTC 1 cut(s) 740
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 2 cut(s) 104, 165
AsuHPI GGTGA 3 cut(s) 370, 586, 824
AsuNHI GCTAGC 1 cut(s) 943
AvaII GGWCC 1 cut(s) 167
BamHI GGATCC 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 1002
BbvI GCAGC 1 cut(s) 824
BccI CCATC 2 cut(s) 16, 331
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciT130I CCWGG 2 cut(s) 63, 444
BcnI CCSGG 2 cut(s) 104, 165
BcoDI GTCTC 3 cut(s) 61, 704, 946
BfaI CTAG 2 cut(s) 252, 944
BglII AGATCT 1 cut(s) 985
BisI GCNGC 1 cut(s) 838
BlsI GCNGC 1 cut(s) 839
Bme1390I CCNGG 4 cut(s) 63, 104, 165, 444
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 4 cut(s) 60, 161, 763, 1045
BmrFI CCNGG 4 cut(s) 63, 104, 165, 444
BmsI GCATC 3 cut(s) 490, 805, 811
BmtI GCTAGC 1 cut(s) 947
BpiI GAAGAC 1 cut(s) 1002
BpuMI CCSGG 2 cut(s) 104, 165
BsaBI GATNNNNATC 1 cut(s) 894
BsaI GGTCTC 1 cut(s) 946
BsaJI CCNNGG 2 cut(s) 62, 163
Bsc4I CCNNNNNNNGG 1 cut(s) 1053
Bse3DI GCAATG 1 cut(s) 778
Bse8I GATNNNNATC 1 cut(s) 894
BseBI CCWGG 2 cut(s) 63, 444
BseDI CCNNGG 2 cut(s) 62, 163
BseGI GGATG 5 cut(s) 304, 323, 334, 481, 649
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 1 cut(s) 1053
BseMI GCAATG 1 cut(s) 778
BseRI GAGGAG 2 cut(s) 1069, 1072
BseXI GCAGC 1 cut(s) 824
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 2 cut(s) 369, 960
Bsh1285I CGRYCG 1 cut(s) 997
BsiEI CGRYCG 1 cut(s) 997
BsiSI CCGG 3 cut(s) 103, 165, 819
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 1 cut(s) 1053
BsmAI GTCTC 3 cut(s) 61, 704, 946
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 715
BsmI GAATGC 1 cut(s) 193
Bso31I GGTCTC 1 cut(s) 946
Bsp143I GATC 4 cut(s) 58, 895, 985, 994
BspACI CCGC 5 cut(s) 234, 369, 840, 960, 1041
BspFNI CGCG 2 cut(s) 369, 960
BspLI GGNNCC 4 cut(s) 60, 161, 763, 1045
BspOI GCTAGC 1 cut(s) 947
BspPI GGATC 2 cut(s) 53, 66
BspQI GCTCTTC 1 cut(s) 903
BspTNI GGTCTC 1 cut(s) 946
BsrDI GCAATG 1 cut(s) 778
BssECI CCNNGG 2 cut(s) 62, 163
BssMI GATC 4 cut(s) 58, 895, 985, 994
Bst2UI CCWGG 2 cut(s) 63, 444
Bst4CI ACNGT 2 cut(s) 183, 1048
Bst6I CTCTTC 2 cut(s) 519, 903
BstC8I GCNNGC 3 cut(s) 244, 405, 945
BstF5I GGATG 5 cut(s) 304, 323, 334, 481, 649
BstFNI CGCG 2 cut(s) 369, 960
BstKTI GATC 4 cut(s) 61, 898, 988, 997
BstMAI GTCTC 3 cut(s) 61, 704, 946
BstMBI GATC 4 cut(s) 58, 895, 985, 994
BstMCI CGRYCG 1 cut(s) 997
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstNI CCWGG 2 cut(s) 63, 444
BstNSI RCATGY 3 cut(s) 246, 690, 783
BstSCI CCNGG 4 cut(s) 61, 102, 163, 442
BstUI CGCG 2 cut(s) 369, 960
BstV1I GCAGC 1 cut(s) 824
BstV2I GAAGAC 1 cut(s) 1002
BstX2I RGATCY 2 cut(s) 58, 985
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 2 cut(s) 58, 985
BtgZI GCGATG 1 cut(s) 924
BtsCI GGATG 5 cut(s) 304, 323, 334, 481, 649
BtsI GCAGTG 1 cut(s) 932
BtsIMutI CAGTG 1 cut(s) 932
Cac8I GCNNGC 3 cut(s) 244, 405, 945
Cfr13I GGNCC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 437, 777, 1005
CspCI CAANNNNNGTGG 2 cut(s) 908, 943
CviAII CATG 6 cut(s) 5, 243, 687, 697, 725, 780
CviJI RGCY 9 cut(s) 75, 160, 403, 407, 532, 590, 764, 837, 931
CviKI_1 RGCY 9 cut(s) 75, 160, 403, 407, 532, 590, 764, 837, 931
CviQI GTAC 3 cut(s) 437, 777, 1005
DpnI GATC 4 cut(s) 60, 897, 987, 996
DpnII GATC 4 cut(s) 58, 895, 985, 994
Eam1104I CTCTTC 2 cut(s) 519, 903
EarI CTCTTC 2 cut(s) 519, 903
Eco31I GGTCTC 1 cut(s) 946
Eco47I GGWCC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 61, 442
EcoT22I ATGCAT 3 cut(s) 6, 244, 660
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 6 cut(s) 8, 246, 690, 700, 728, 783
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 715
FatI CATG 6 cut(s) 4, 242, 686, 696, 724, 779
Fnu4HI GCNGC 1 cut(s) 838
FokI GGATG 5 cut(s) 310, 311, 321, 468, 656
Fsp4HI GCNGC 1 cut(s) 838
FspBI CTAG 2 cut(s) 252, 944
GluI GCNGC 1 cut(s) 838
HapII CCGG 3 cut(s) 103, 165, 819
Hin1II CATG 6 cut(s) 8, 246, 690, 700, 728, 783
HincII GTYRAC 2 cut(s) 100, 720
HindII GTYRAC 2 cut(s) 100, 720
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 6 cut(s) 47, 214, 669, 716, 874, 1024
HpaII CCGG 3 cut(s) 103, 165, 819
HphI GGTGA 3 cut(s) 370, 586, 824
Hpy166II GTNNAC 2 cut(s) 100, 720
Hpy188III TCNNGA 9 cut(s) 44, 89, 218, 252, 416, 476, 878, 899, 983
Hpy8I GTNNAC 2 cut(s) 100, 720
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 513, 571
HpyCH4III ACNGT 2 cut(s) 183, 1048
HpyCH4IV ACGT 2 cut(s) 70, 1007
HpyCH4V TGCA 7 cut(s) 4, 191, 242, 431, 658, 991, 1033
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpySE526I ACGT 2 cut(s) 70, 1007
Hsp92II CATG 6 cut(s) 8, 246, 690, 700, 728, 783
Kzo9I GATC 4 cut(s) 58, 895, 985, 994
LguI GCTCTTC 1 cut(s) 903
LmnI GCTCC 3 cut(s) 165, 761, 1059
Lsp1109I GCAGC 1 cut(s) 824
LweI GCATC 3 cut(s) 490, 805, 811
MaeI CTAG 2 cut(s) 252, 944
MaeII ACGT 2 cut(s) 70, 1007
MaeIII GTNAC 2 cut(s) 309, 610
MalI GATC 4 cut(s) 60, 897, 987, 996
MboI GATC 4 cut(s) 58, 895, 985, 994
MboII GAAGA 7 cut(s) 223, 530, 536, 920, 927, 986, 1002
MfeI CAATTG 3 cut(s) 432, 785, 1028
MflI RGATCY 2 cut(s) 58, 985
MluCI AATT 9 cut(s) 328, 383, 432, 487, 568, 736, 785, 859, 1028
MlyI GAGTC 2 cut(s) 725, 868
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 8 cut(s) 276, 375, 415, 508, 573, 966, 1047, 1050
Mph1103I ATGCAT 3 cut(s) 6, 244, 660
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 4 cut(s) 110, 138, 152, 755
MslI CAYNNNNRTG 3 cut(s) 93, 807, 810
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 3 cut(s) 103, 165, 819
MspR9I CCNGG 4 cut(s) 63, 104, 165, 444
MunI CAATTG 3 cut(s) 432, 785, 1028
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 2 cut(s) 63, 444
MvnI CGCG 2 cut(s) 369, 960
MwoI GCNNNNNNNGC 1 cut(s) 596
NciI CCSGG 2 cut(s) 104, 165
NdeII GATC 4 cut(s) 58, 895, 985, 994
NheI GCTAGC 1 cut(s) 943
NlaIII CATG 6 cut(s) 8, 246, 690, 700, 728, 783
NlaIV GGNNCC 4 cut(s) 60, 161, 763, 1045
NsiI ATGCAT 3 cut(s) 6, 244, 660
NspI RCATGY 3 cut(s) 246, 690, 783
PaeI GCATGC 1 cut(s) 246
PciI ACATGT 2 cut(s) 686, 779
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 1 cut(s) 1019
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 4 cut(s) 47, 214, 669, 1024
PflFI GACNNNGTC 2 cut(s) 95, 715
PkrI GCNGC 1 cut(s) 839
Ple19I CGATCG 1 cut(s) 997
PleI GAGTC 2 cut(s) 724, 868
PpsI GAGTC 2 cut(s) 724, 868
PscI ACATGT 2 cut(s) 686, 779
PsiI TTATAA 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 61, 442
PspGI CCWGG 2 cut(s) 61, 442
PspN4I GGNNCC 4 cut(s) 60, 161, 763, 1045
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 2 cut(s) 58, 985
PsyI GACNNNGTC 2 cut(s) 95, 715
PvuI CGATCG 1 cut(s) 997
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 3 cut(s) 438, 778, 1006
RsaNI GTAC 3 cut(s) 437, 777, 1005
RseI CAYNNNNRTG 3 cut(s) 93, 807, 810
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 4 cut(s) 110, 138, 152, 755
SatI GCNGC 1 cut(s) 838
Sau3AI GATC 4 cut(s) 58, 895, 985, 994
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 2 cut(s) 725, 868
ScrFI CCNGG 4 cut(s) 63, 104, 165, 444
SetI ASST 9 cut(s) 73, 287, 405, 505, 534, 582, 839, 958, 1010
SfaNI GCATC 3 cut(s) 490, 805, 811
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 3 cut(s) 93, 807, 810
SphI GCATGC 1 cut(s) 246
Sse9I AATT 9 cut(s) 328, 383, 432, 487, 568, 736, 785, 859, 1028
SsiI CCGC 5 cut(s) 234, 369, 840, 960, 1041
SspMI CTAG 2 cut(s) 252, 944
StyD4I CCNGG 4 cut(s) 61, 102, 163, 442
TaaI ACNGT 2 cut(s) 183, 1048
TaiI ACGT 2 cut(s) 73, 1010
TaqI TCGA 4 cut(s) 648, 855, 1013, 1022
TasI AATT 9 cut(s) 328, 383, 432, 487, 568, 736, 785, 859, 1028
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 4 cut(s) 47, 214, 669, 1024
Tru1I TTAA 4 cut(s) 110, 138, 152, 755
Tru9I TTAA 4 cut(s) 110, 138, 152, 755
TscAI CASTG 1 cut(s) 932
TseI GCWGC 1 cut(s) 837
TspDTI ATGAA 9 cut(s) 29, 144, 224, 315, 323, 537, 927, 1008, 1065
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 1 cut(s) 932
Tth111I GACNNNGTC 2 cut(s) 95, 715
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 1 cut(s) 568
XbaI TCTAGA 1 cut(s) 251
XceI RCATGY 3 cut(s) 246, 690, 783
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 2 cut(s) 252, 944
Zsp2I ATGCAT 3 cut(s) 6, 244, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.