Rroxscaffold_4G00319990

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0021169
Physical Location & Seq
Reverse (-)
48250063 .. 48252453
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319990.1

Sequence Viewer

Length: 1350 bp
ATGCATGACTTATTAGAACAGATGGGTAAGGAAATTGTTCATCAAGAATCTCCTATAGATCCCGGCGTACGTAGCCGATTGTGGTTTCACGACGATGTCTACCGGGTTTTAACAGAAAACACAGGAAGTGAAAAAGTTAAAGGCATCATCTTAAAATGGCTCCCCGAACCATATAAGATACAGTTGAATGCAAAATGCTTCTCAAAGATGAAGAATCTTGAACTTCTTATAAGCCGTAATGCATGCCTTTTCGGAGAAGTGGATTATCTCCCCAACAGATTGAGGTTGCTTGATTGGGATGAATGTGAGTTACAATCTTTGCCATCCAATTTTCATCCAAAGGAACTTGTTGTTTTCAAAATGCCTCGCGAGCCTAATCACTCAATTGGGAAAAGGATTTCAGATCTCTCGGGAAACAATTTTGAAAGTATTCCGGTATGCATTACCAAATTTGTCAACTTGCGTACCCTTAAATTGTCTAGTTGCAAGAAGCTCCGAGATATTCCAGAACTTCCACCAAATGTAGAAGTTGTAAACGTGTATGATTGCACAGCACTGAAAAGTTATCCAAATGTGTCAATTATATTGGAACAAAATGTCTTCCGGGGTTTAAGGGATCTGCAGTTGATGAATTGCCGAACACTATGTGGGAATCATGGCTTTGACGAGGCAAAGATGGAATGCTTTTTAATGAATCAGCCGGCTTGTTGTCGTGATGAGGACTTTGCAATTGTACTTCCAGGCAATCAAGTTCCAAAGTGGTTCAAGTATCGTGAGCATCCAAATTACATATATATACCTTCAACTTCAGAGGAAGATGAAGAGAAGCTTGATATATGTGAAGTTTCTATTGGAGTTCCTCCAAATTCGGTGAAGGTTAGATTGGCTCTCTGTGCTGTTTTTGAAGTTACCCAAAATAATGGTGGTCTTTTTTCTATGGATGTCGATATTATGCATAATGGGAGATTCTTGGGTTATAGGACATGTATTGACATGACTAATATAGAGACAGAGTCAACCCATGTGTCCCTGAATTATTTCAATGTGTCCTTAAATGGAGCCGAAAGCAATGGTACATGTCAATTGATATTATTTTGGTACCGTAATGGTGATGCCGCATTGTCGTTGAAAAGCTGCGGATTTCACATTGTCGATAATTGTGGAGTTATGACTCTTGATAATAATGATTTAGATCAAGAAAAGGAAGAGCAAGAGCAAGAGCAGTGGCTTTCTTCATCGCTAGCAAAGAGACCTCGCGGAGAAGCAGGGGAAGATAATCAAGATCTGCACGACGGTGTTGTACGTCTTCGACTTCATCGAATCAATTGCAAACAAGCGGAACCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

449

Amino Acids

51.38

Weight (kDa)

5.36

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021169)

Species Orthologous Gene IDs
malus_domestica MD02G1113200.v1.1
rosa_laevigata RLG00000029814
rosa_roxburghii Rroxscaffold_4G00319990 Rroxscaffold_4G00320110
rosa_samantha Rh1BG084700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 230
Acc65I GGTACC 1 cut(s) 1098
AccB1I GGYRCC 1 cut(s) 1098
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 369, 1257
AciI CCGC 4 cut(s) 1116, 1137, 1257, 1337
AclWI GGATC 2 cut(s) 53, 624
AcsI RAATTY 2 cut(s) 449, 865
AcuI CTGAAG 1 cut(s) 792
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 6 cut(s) 69, 466, 735, 1075, 1100, 1302
AflIII ACRYGT 3 cut(s) 537, 983, 1076
AgsI TTSAA 9 cut(s) 187, 221, 358, 425, 766, 804, 905, 1042, 1129
AjnI CCWGG 1 cut(s) 739
AjuI GAANNNNNNNTTGG 4 cut(s) 834, 866, 866, 898
AleI CACNNNNGTG 1 cut(s) 1293
AluBI AGCT 3 cut(s) 493, 829, 1134
AluI AGCT 3 cut(s) 493, 829, 1134
Alw26I GTCTC 2 cut(s) 1001, 1243
AlwI GGATC 2 cut(s) 53, 624
Ama87I CYCGRG 1 cut(s) 409
ApeKI GCWGC 1 cut(s) 1134
ApoI RAATTY 2 cut(s) 449, 865
Asp700I GAANNNNTTC 3 cut(s) 36, 429, 1037
Asp718I GGTACC 1 cut(s) 1098
AsuC2I CCSGG 3 cut(s) 63, 104, 605
AsuHPI GGTGA 2 cut(s) 883, 1121
AsuNHI GCTAGC 1 cut(s) 1240
AvaI CYCGRG 1 cut(s) 409
BaeI ACNNNNGTAYC 2 cut(s) 448, 481
BanI GGYRCC 1 cut(s) 1098
BbsI GAAGAC 2 cut(s) 592, 1298
BbvI GCAGC 1 cut(s) 1121
BccI CCATC 3 cut(s) 16, 331, 670
BceAI ACGGC 1 cut(s) 219
BcgI CGANNNNNNTGC 2 cut(s) 1308, 1342
BciT130I CCWGG 1 cut(s) 741
BcnI CCSGG 3 cut(s) 63, 104, 605
BcoDI GTCTC 2 cut(s) 1001, 1243
BfaI CTAG 2 cut(s) 480, 1241
BfmI CTRYAG 2 cut(s) 54, 620
BglII AGATCT 2 cut(s) 403, 1282
BisI GCNGC 2 cut(s) 1116, 1135
BlsI GCNGC 2 cut(s) 1117, 1136
Bme1390I CCNGG 4 cut(s) 63, 104, 605, 741
BmeT110I CYCGRG 1 cut(s) 409
BmiI GGNNCC 4 cut(s) 161, 1060, 1100, 1341
BmrFI CCNGG 4 cut(s) 63, 104, 605, 741
BmsI GCATC 3 cut(s) 153, 787, 1102
BmtI GCTAGC 1 cut(s) 1244
BpiI GAAGAC 2 cut(s) 592, 1298
BpuMI CCSGG 3 cut(s) 63, 104, 605
BsaAI YACGTR 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 1191
BsaI GGTCTC 1 cut(s) 1243
BsaJI CCNNGG 1 cut(s) 604
BsaWI WCCGGW 1 cut(s) 433
Bse118I RCCGGY 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 1075
Bse8I GATNNNNATC 1 cut(s) 1191
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 1 cut(s) 604
BseGI GGATG 5 cut(s) 304, 323, 334, 778, 946
BseJI GATNNNNATC 1 cut(s) 1191
BseMI GCAATG 1 cut(s) 1075
BseXI GCAGC 1 cut(s) 1121
BsgI GTGCAG 1 cut(s) 1271
Bsh1236I CGCG 2 cut(s) 369, 1257
BshNI GGYRCC 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 409
BsiSI CCGG 5 cut(s) 63, 103, 434, 604, 701
BsiWI CGTACG 1 cut(s) 67
BslFI GGGAC 1 cut(s) 1012
BsmAI GTCTC 2 cut(s) 1001, 1243
BsmFI GGGAC 1 cut(s) 1012
BsmI GAATGC 2 cut(s) 193, 686
Bso31I GGTCTC 1 cut(s) 1243
BsoBI CYCGRG 1 cut(s) 409
Bsp143I GATC 5 cut(s) 58, 403, 616, 1192, 1282
Bsp68I TCGCGA 1 cut(s) 369
BspACI CCGC 4 cut(s) 1116, 1137, 1257, 1337
BspFNI CGCG 2 cut(s) 369, 1257
BspLI GGNNCC 4 cut(s) 161, 1060, 1100, 1341
BspMAI CTGCAG 1 cut(s) 624
BspOI GCTAGC 1 cut(s) 1244
BspPI GGATC 2 cut(s) 53, 624
BspQI GCTCTTC 1 cut(s) 1200
BspT107I GGYRCC 1 cut(s) 1098
BspTNI GGTCTC 1 cut(s) 1243
BsrDI GCAATG 1 cut(s) 1075
BsrFI RCCGGY 1 cut(s) 700
BssAI RCCGGY 1 cut(s) 700
BssECI CCNNGG 1 cut(s) 604
BssMI GATC 5 cut(s) 58, 403, 616, 1192, 1282
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 4 cut(s) 183, 1103, 1295, 1344
Bst6I CTCTTC 2 cut(s) 816, 1200
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 244, 371, 702, 1242
BstF5I GGATG 5 cut(s) 304, 323, 334, 778, 946
BstFNI CGCG 2 cut(s) 369, 1257
BstKTI GATC 5 cut(s) 61, 406, 619, 1195, 1285
BstMAI GTCTC 2 cut(s) 1001, 1243
BstMBI GATC 5 cut(s) 58, 403, 616, 1192, 1282
BstMWI GCNNNNNNNGC 3 cut(s) 72, 370, 893
BstNI CCWGG 1 cut(s) 741
BstNSI RCATGY 3 cut(s) 246, 987, 1080
BstSCI CCNGG 4 cut(s) 61, 102, 603, 739
BstSFI CTRYAG 2 cut(s) 54, 620
BstSNI TACGTA 1 cut(s) 71
BstUI CGCG 2 cut(s) 369, 1257
BstV1I GCAGC 1 cut(s) 1121
BstV2I GAAGAC 2 cut(s) 592, 1298
BstX2I RGATCY 4 cut(s) 58, 403, 616, 1282
BstXI CCANNNNNNTGG 1 cut(s) 920
BstYI RGATCY 4 cut(s) 58, 403, 616, 1282
BtgZI GCGATG 1 cut(s) 1221
BtsCI GGATG 5 cut(s) 304, 323, 334, 778, 946
BtsI GCAGTG 1 cut(s) 1229
BtsIMutI CAGTG 2 cut(s) 554, 1229
BtuMI TCGCGA 1 cut(s) 369
Cac8I GCNNGC 4 cut(s) 244, 371, 702, 1242
Cfr10I RCCGGY 1 cut(s) 700
Csp6I GTAC 6 cut(s) 68, 465, 734, 1074, 1099, 1301
CspCI CAANNNNNGTGG 2 cut(s) 1205, 1240
CviAII CATG 7 cut(s) 5, 243, 656, 984, 994, 1022, 1077
CviQI GTAC 6 cut(s) 68, 465, 734, 1074, 1099, 1301
DpnI GATC 5 cut(s) 60, 405, 618, 1194, 1284
DpnII GATC 5 cut(s) 58, 403, 616, 1192, 1282
DraIII CACNNNGTG 1 cut(s) 647
Eam1104I CTCTTC 2 cut(s) 816, 1200
EarI CTCTTC 2 cut(s) 816, 1200
Eco105I TACGTA 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 1243
Eco57I CTGAAG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 739
EcoT22I ATGCAT 4 cut(s) 6, 244, 443, 957
FaeI CATG 7 cut(s) 8, 246, 659, 987, 997, 1025, 1080
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 1 cut(s) 1012
FatI CATG 7 cut(s) 4, 242, 655, 983, 993, 1021, 1076
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 2 cut(s) 1116, 1135
FokI GGATG 5 cut(s) 310, 311, 321, 765, 953
Fsp4HI GCNGC 2 cut(s) 1116, 1135
FspBI CTAG 2 cut(s) 480, 1241
GluI GCNGC 2 cut(s) 1116, 1135
HapII CCGG 5 cut(s) 63, 103, 434, 604, 701
Hin1II CATG 7 cut(s) 8, 246, 659, 987, 997, 1025, 1080
HincII GTYRAC 2 cut(s) 457, 1017
HindII GTYRAC 2 cut(s) 457, 1017
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 8 cut(s) 47, 214, 652, 694, 966, 1013, 1171, 1320
HpaII CCGG 5 cut(s) 63, 103, 434, 604, 701
HphI GGTGA 2 cut(s) 883, 1121
Hpy166II GTNNAC 4 cut(s) 100, 457, 535, 1017
Hpy188I TCNGA 4 cut(s) 254, 403, 497, 811
Hpy8I GTNNAC 4 cut(s) 100, 457, 535, 1017
Hpy99I CGWCG 2 cut(s) 95, 1295
HpyAV CCTTC 2 cut(s) 810, 868
HpyCH4III ACNGT 4 cut(s) 183, 1103, 1295, 1344
HpyCH4IV ACGT 3 cut(s) 70, 537, 1303
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 370, 893
HpySE526I ACGT 3 cut(s) 70, 537, 1303
Hsp92II CATG 7 cut(s) 8, 246, 659, 987, 997, 1025, 1080
KpnI GGTACC 1 cut(s) 1102
KroI GCCGGC 1 cut(s) 700
KroNI GCCGGC 1 cut(s) 702
Kzo9I GATC 5 cut(s) 58, 403, 616, 1192, 1282
LguI GCTCTTC 1 cut(s) 1200
LmnI GCTCC 3 cut(s) 165, 498, 1058
Lsp1109I GCAGC 1 cut(s) 1121
LweI GCATC 3 cut(s) 153, 787, 1102
MaeI CTAG 2 cut(s) 480, 1241
MaeII ACGT 3 cut(s) 70, 537, 1303
MaeIII GTNAC 2 cut(s) 309, 907
MalI GATC 5 cut(s) 60, 405, 618, 1194, 1284
MboI GATC 5 cut(s) 58, 403, 616, 1192, 1282
MboII GAAGA 8 cut(s) 223, 592, 827, 833, 1217, 1224, 1283, 1298
MfeI CAATTG 4 cut(s) 384, 729, 1082, 1324
MflI RGATCY 4 cut(s) 58, 403, 616, 1282
MlyI GAGTC 2 cut(s) 1022, 1165
MnlI CCTC 7 cut(s) 276, 375, 661, 712, 805, 870, 1263
Mph1103I ATGCAT 4 cut(s) 6, 244, 443, 957
MroNI GCCGGC 1 cut(s) 700
MroXI GAANNNNTTC 3 cut(s) 36, 429, 1037
MseI TTAA 7 cut(s) 110, 138, 152, 471, 611, 689, 1052
MslI CAYNNNNRTG 2 cut(s) 93, 1293
MspI CCGG 5 cut(s) 63, 103, 434, 604, 701
MspR9I CCNGG 4 cut(s) 63, 104, 605, 741
MunI CAATTG 4 cut(s) 384, 729, 1082, 1324
Mva1269I GAATGC 2 cut(s) 193, 686
MvaI CCWGG 1 cut(s) 741
MvnI CGCG 2 cut(s) 369, 1257
MwoI GCNNNNNNNGC 3 cut(s) 72, 370, 893
NaeI GCCGGC 1 cut(s) 702
NciI CCSGG 3 cut(s) 63, 104, 605
NdeII GATC 5 cut(s) 58, 403, 616, 1192, 1282
NgoMIV GCCGGC 1 cut(s) 700
NheI GCTAGC 1 cut(s) 1240
NlaIII CATG 7 cut(s) 8, 246, 659, 987, 997, 1025, 1080
NlaIV GGNNCC 4 cut(s) 161, 1060, 1100, 1341
NruI TCGCGA 1 cut(s) 369
NsiI ATGCAT 4 cut(s) 6, 244, 443, 957
NspI RCATGY 3 cut(s) 246, 987, 1080
OliI CACNNNNGTG 1 cut(s) 1293
PaeI GCATGC 1 cut(s) 246
PciI ACATGT 2 cut(s) 983, 1076
PciSI GCTCTTC 1 cut(s) 1200
PcsI WCGNNNNNNNCGW 1 cut(s) 1315
PctI GAATGC 2 cut(s) 193, 686
PdiI GCCGGC 1 cut(s) 702
PdmI GAANNNNTTC 3 cut(s) 36, 429, 1037
PfeI GAWTC 6 cut(s) 47, 214, 652, 694, 966, 1320
Pfl23II CGTACG 1 cut(s) 67
PflFI GACNNNGTC 2 cut(s) 95, 1012
PkrI GCNGC 2 cut(s) 1117, 1136
PleI GAGTC 2 cut(s) 1021, 1165
PpsI GAGTC 2 cut(s) 1021, 1165
Ppu21I YACGTR 1 cut(s) 71
PscI ACATGT 2 cut(s) 983, 1076
PsiI TTATAA 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspLI CGTACG 1 cut(s) 67
PspN4I GGNNCC 4 cut(s) 161, 1060, 1100, 1341
PstI CTGCAG 1 cut(s) 624
PsuI RGATCY 4 cut(s) 58, 403, 616, 1282
PsyI GACNNNGTC 2 cut(s) 95, 1012
RruI TCGCGA 1 cut(s) 369
RsaI GTAC 6 cut(s) 69, 466, 735, 1075, 1100, 1302
RsaNI GTAC 6 cut(s) 68, 465, 734, 1074, 1099, 1301
RseI CAYNNNNRTG 2 cut(s) 93, 1293
SapI GCTCTTC 1 cut(s) 1200
SaqAI TTAA 7 cut(s) 110, 138, 152, 471, 611, 689, 1052
SatI GCNGC 2 cut(s) 1116, 1135
Sau3AI GATC 5 cut(s) 58, 403, 616, 1192, 1282
SchI GAGTC 2 cut(s) 1022, 1165
ScrFI CCNGG 4 cut(s) 63, 104, 605, 741
SfaNI GCATC 3 cut(s) 153, 787, 1102
SfcI CTRYAG 2 cut(s) 54, 620
SmiMI CAYNNNNRTG 2 cut(s) 93, 1293
SnaBI TACGTA 1 cut(s) 71
SphI GCATGC 1 cut(s) 246
SsiI CCGC 4 cut(s) 1116, 1137, 1257, 1337
SspMI CTAG 2 cut(s) 480, 1241
StyD4I CCNGG 4 cut(s) 61, 102, 603, 739
TaaI ACNGT 4 cut(s) 183, 1103, 1295, 1344
TaiI ACGT 3 cut(s) 73, 540, 1306
TaqI TCGA 4 cut(s) 945, 1152, 1309, 1318
TatI WGTACW 1 cut(s) 733
TauI GCSGC 1 cut(s) 1118
TfiI GAWTC 6 cut(s) 47, 214, 652, 694, 966, 1320
Tru1I TTAA 7 cut(s) 110, 138, 152, 471, 611, 689, 1052
Tru9I TTAA 7 cut(s) 110, 138, 152, 471, 611, 689, 1052
TscAI CASTG 2 cut(s) 561, 1229
TseI GCWGC 1 cut(s) 1134
TspDTI ATGAA 9 cut(s) 29, 224, 315, 323, 644, 707, 834, 1224, 1304
TspRI CASTG 2 cut(s) 561, 1229
Tth111I GACNNNGTC 2 cut(s) 95, 1012
XapI RAATTY 2 cut(s) 449, 865
XceI RCATGY 3 cut(s) 246, 987, 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 3 cut(s) 36, 429, 1037
XspI CTAG 2 cut(s) 480, 1241
Zsp2I ATGCAT 4 cut(s) 6, 244, 443, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.