Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005885

Physical Location & Seq

Genomic Coordinates Reverse (-)

10771789 .. 10775261

Gene Structure 3473 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1133100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGACCGACAACACGGCCGATAACAACCGGGTCGAACCGGAGGACCCGTTCCTCGTAGCGTATCAGCCATCGGAGCTCCGAATCGCCTCGGAGTTTCTCTCAACCTGGCTCTCCTTTCTCACCAGAGATCTTTGTCCACACTGCTCCGCCAAACTCGCCGATCGCGTCCGCTCTCTCGGCCCAGAACTTGGTGGGGATTCGAAACCCGTTCACCCGGATGAGAATTCGGGAGATTTGAATACGGAGAGCAAGGAATTGCAGGTGGGTAGTGATTATGATAATGGTGAGGAGCATCATGATGATGACACAAAGTCACTAGGTAATTGGGACGACGGAGCTCAGGGGATGTCGGAACCCGACCCAGAAACTTCTAACAGTGGATTGGCCCCCTTATTAGAGACTCCAAGCCCTCGAATGTCGTGGGCCGATATGGCACAGGAAGATGAGCTGGAAGAAGAGGAAGAGCATGAGTTGAATAAGCGGGTGGTCAATGTGAACGAGGCGACTGGAAAGTTGAGGATTACGAAGTCTACATTGTCTAGGGAGCAGAGGGAACACTTTAGGATCACGAATGTTAGGAGGAAGAAAGATTATATTTGTTTGGAGAGGATTAATGGGAAATTGGTTAACATTGTCGATGGGCTTGAGCTCCACACTGGTATCTTCAGTGCGGCGGAACAGAATAGGATTGTTGATTATGTTTATAAGCTTCAAGAGATGGGCAGGAATGGTTTATTAAAAGCACGTACGTATACAGCACCTCAAAAGTGGATTAGGGGCAAGCGACGAGTAACCATTCAGTTCGGCTGTTGCTACAATTATGCAGTGGATAAGTATGGTAATCCACCTGGTATCCTACGCGATGATGTTGTCGATCCCATACCTCAACTTTTCAAGGTGATCATTAGGAGGCTGGTAAAATGGCATGTGCTTCCCCCGACTTGTGTACCTGATAGTTGCATCGTCAATGTATATGATGAAGGGGACTGTATACCTCCACATATTGACAACCATGATTTTGTTCGACCTTTCTGTACCGTGTCCTTTCTTGGTGAGTGCAACATAGTTTTTGGTTCAAACTTAAAGATCATTGGTCCTGGCGAGTTTGAAGGTTCATTTGCCATTCCCTTGCCAGTTGGGTCTGTTCTGGTTTTAAACGGAAGGGGGGCTGATGTAGCTAAGCATTGTGTGCCTCCAGTACCTACAAAACGGATATCAATCACATTTAGAAGAATGGATGAAACCAAACGGCCGAATGGTTATGTTCCAGAACCTGATCTACAGGATCTTCAACCACTATCCTTTGAAGAGGACAAGAAAACTAGATTAAATTCACCTAGGAGCCAACCTCAGATGAGGAGGCAGCCAATTAGGGATCAGATGAGGAGGCAGCCAATTAGGGGATCTGCTGACAGACGTGAGTTCCACTCAGAAATTAGGGGATCTGCCGACAGACATGAGTTCTACTCAGAGCCCCGAGAGTCAAGTTGGAGTCGACGTGGATCTGGAAATAGGTGGAACCGGGGAAGGGTCAACGTGAATTTTGAGAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0007276 GO:0007283 GO:0008104 GO:0008150 GO:0008152 GO:0009451 GO:0009628 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016491 GO:0016604 GO:0016607 GO:0016705 GO:0016706 GO:0019953 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032451 GO:0032501 GO:0032504 GO:0033036 GO:0034613 GO:0034641 GO:0035513 GO:0035515 GO:0035553 GO:0036293 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044703 GO:0045184 GO:0046483 GO:0046907 GO:0048232 GO:0048609 GO:0050657 GO:0050658 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051213 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0055114 GO:0070013 GO:0070482 GO:0070727 GO:0070988 GO:0070989 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy_2

Protein Analysis

518

Amino Acids

58.85

Weight (kDa)

5.82

Isoelectric Point (pI)

52.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2OG-FeII_Oxy_2	PF13532	216 - 410	2.3e-12	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005885)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17970 AT2G17970 AT2G17970 AT2G17970 AT2G17970 AT2G17970 AT2G17970 AT2G17970 AT2G17970 AT2G17970
fragaria_vesca	FvH4_1g11920 FvH4_1g11920 FvH4_1g11920
malus_domestica	MD02G1133100.v1.1 MD15G1245500.v1.1
prunus_persica	Prupe.7G167600_v2.0.a1
pyrus_communis	pycom02g10460 pycom15g21660
rosa_chinensis	RchiOBHm_Chr2g0099521
rosa_laevigata	RLG00000016889
rosa_multiflora	Rmu_sc0002542.1_g000018
rosa_roxburghii	Rroxscaffold_2G00143000
rosa_rugosa	Rorug02G0080300
rosa_samantha	Rh2AG130200 Rh2BG133300 Rh2CG133900 Rh2DG134200
rosa_wichuraiana	Rw2G010040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	705
AarI	CACCTGC	1 cut(s)	250
Acc36I	ACCTGC	1 cut(s)	250
AccB7I	CCANNNNNTGG	1 cut(s)	188
AccBSI	CCGCTC	1 cut(s)	171
AccI	GTMKAC	4 cut(s)	530, 752, 991, 1495
AccII	CGCG	2 cut(s)	165, 861
AciI	CCGC	5 cut(s)	147, 169, 481, 671, 674
AclWI	GGATC	7 cut(s)	572, 869, 1293, 1383, 1411, 1450, 1510
AcoI	YGGCCR	2 cut(s)	15, 1250
AcsI	RAATTY	3 cut(s)	223, 1330, 1540
AcuI	CTGAAG	1 cut(s)	649
AfaI	GTAC	4 cut(s)	748, 948, 1036, 1200
AfiI	CCNNNNNNNGG	3 cut(s)	188, 1400, 1528
AgsI	TTSAA	8 cut(s)	238, 475, 713, 895, 1077, 1109, 1292, 1307
AhdI	GACNNNNNGTC	1 cut(s)	310
AjiI	CACGTC	2 cut(s)	1418, 1499
AjnI	CCWGG	3 cut(s)	104, 847, 1096
AloI	GAACNNNNNNTCC	2 cut(s)	32, 64
AluBI	AGCT	7 cut(s)	76, 338, 448, 649, 709, 1178, 1551
AluI	AGCT	7 cut(s)	76, 338, 448, 649, 709, 1178, 1551
Alw21I	GWGCWC	3 cut(s)	78, 340, 651
Alw26I	GTCTC	1 cut(s)	392
AlwI	GGATC	7 cut(s)	572, 869, 1293, 1383, 1411, 1450, 1510
AlwNI	CAGNNNCTG	1 cut(s)	1274
Ama87I	CYCGRG	1 cut(s)	1476
AoxI	GGCC	5 cut(s)	15, 178, 384, 423, 1250
ApeKI	GCWGC	2 cut(s)	1363, 1390
ApoI	RAATTY	3 cut(s)	223, 1330, 1540
AseI	ATTAAT	1 cut(s)	612
AspA2I	CCTAGG	1 cut(s)	1337
AspS9I	GGNCC	5 cut(s)	43, 179, 385, 423, 1094
AsuC2I	CCSGG	3 cut(s)	29, 215, 1523
AsuHPI	GGTGA	6 cut(s)	112, 203, 296, 910, 1064, 1326
AsuII	TTCGAA	1 cut(s)	200
AvaI	CYCGRG	1 cut(s)	1476
AvaII	GGWCC	2 cut(s)	43, 1094
AvrII	CCTAGG	1 cut(s)	1337
BaeI	ACNNNNGTAYC	4 cut(s)	643, 676, 930, 963
BanII	GRGCYC	4 cut(s)	78, 340, 651, 1476
Bbv12I	GWGCWC	3 cut(s)	78, 340, 651
BbvI	GCAGC	2 cut(s)	1375, 1402
BccI	CCATC	3 cut(s)	76, 632, 712
BceAI	ACGGC	2 cut(s)	30, 1265
BciT130I	CCWGG	3 cut(s)	106, 849, 1098
BciVI	GTATCC	1 cut(s)	863
BclI	TGATCA	1 cut(s)	900
BcnI	CCSGG	3 cut(s)	29, 215, 1523
BcoDI	GTCTC	1 cut(s)	392
BfaI	CTAG	5 cut(s)	317, 540, 1323, 1338, 1552
BfmI	CTRYAG	1 cut(s)	1280
BfuAI	ACCTGC	1 cut(s)	250
BfuI	GTATCC	1 cut(s)	863
BglI	GCCNNNNNGGC	1 cut(s)	431
BglII	AGATCT	1 cut(s)	127
BisI	GCNGC	3 cut(s)	672, 1364, 1391
BlnI	CCTAGG	1 cut(s)	1337
BlpI	GCTNAGC	1 cut(s)	1179
BlsI	GCNGC	3 cut(s)	673, 1365, 1392
Bme1390I	CCNGG	6 cut(s)	29, 106, 215, 849, 1098, 1523
Bme18I	GGWCC	2 cut(s)	43, 1094
BmeRI	GACNNNNNGTC	1 cut(s)	310
BmeT110I	CYCGRG	1 cut(s)	1476
BmgBI	CACGTC	2 cut(s)	1418, 1499
BmgT120I	GGNCC	5 cut(s)	43, 179, 385, 423, 1094
BmiI	GGNNCC	5 cut(s)	45, 354, 387, 1343, 1520
BmrFI	CCNGG	6 cut(s)	29, 106, 215, 849, 1098, 1523
BmsI	GCATC	2 cut(s)	301, 969
BplI	GAGNNNNNCTC	8 cut(s)	83, 115, 1333, 1365, 1412, 1444, 1451, 1483
BpmI	CTGGAG	1 cut(s)	1179
Bpu10I	CCTNAGC	1 cut(s)	339
Bpu1102I	GCTNAGC	1 cut(s)	1179
Bpu14I	TTCGAA	1 cut(s)	200
BpuEI	CTTGAG	1 cut(s)	665
BpuMI	CCSGG	3 cut(s)	29, 215, 1523
BsaAI	YACGTR	2 cut(s)	746, 750
BsaBI	GATNNNNATC	1 cut(s)	1217
BsaJI	CCNNGG	3 cut(s)	87, 1337, 1522
BsaWI	WCCGGW	1 cut(s)	37
BsaXI	ACNNNNNCTCC	4 cut(s)	32, 62, 1483, 1513
Bsc4I	CCNNNNNNNGG	3 cut(s)	188, 1400, 1528
Bse1I	ACTGG	4 cut(s)	511, 661, 1133, 1196
Bse8I	GATNNNNATC	1 cut(s)	1217
BseBI	CCWGG	3 cut(s)	106, 849, 1098
BseDI	CCNNGG	3 cut(s)	87, 1337, 1522
BseGI	GGATG	3 cut(s)	223, 351, 1243
BseJI	GATNNNNATC	1 cut(s)	1217
BseLI	CCNNNNNNNGG	3 cut(s)	188, 1400, 1528
BseMII	CTCAG	4 cut(s)	353, 1364, 1443, 1482
BseNI	ACTGG	4 cut(s)	511, 661, 1133, 1196
BseRI	GAGGAG	3 cut(s)	302, 1372, 1399
BseX3I	CGGCCG	2 cut(s)	15, 1250
BseXI	GCAGC	2 cut(s)	1375, 1402
Bsh1236I	CGCG	2 cut(s)	165, 861
Bsh1285I	CGRYCG	3 cut(s)	18, 163, 1253
BshFI	GGCC	5 cut(s)	17, 180, 386, 425, 1252
BsiEI	CGRYCG	3 cut(s)	18, 163, 1253
BsiHKAI	GWGCWC	3 cut(s)	78, 340, 651
BsiHKCI	CYCGRG	1 cut(s)	1476
BsiSI	CCGG	4 cut(s)	28, 38, 215, 1522
BsiWI	CGTACG	1 cut(s)	746
BslFI	GGGAC	2 cut(s)	341, 998
BslI	CCNNNNNNNGG	3 cut(s)	188, 1400, 1528
BsmAI	GTCTC	1 cut(s)	392
BsmFI	GGGAC	2 cut(s)	341, 998
BsnI	GGCC	5 cut(s)	17, 180, 386, 425, 1252
BsoBI	CYCGRG	1 cut(s)	1476
Bsp119I	TTCGAA	1 cut(s)	200
Bsp1286I	GDGCHC	4 cut(s)	78, 340, 651, 1476
Bsp1720I	GCTNAGC	1 cut(s)	1179
BspACI	CCGC	5 cut(s)	147, 169, 481, 671, 674
BspANI	GGCC	5 cut(s)	17, 180, 386, 425, 1252
BspCNI	CTCAG	4 cut(s)	352, 1363, 1442, 1481
BspFNI	CGCG	2 cut(s)	165, 861
BspHI	TCATGA	1 cut(s)	295
BspLI	GGNNCC	5 cut(s)	45, 354, 387, 1343, 1520
BspMI	ACCTGC	1 cut(s)	250
BspPI	GGATC	7 cut(s)	572, 869, 1293, 1383, 1411, 1450, 1510
BspQI	GCTCTTC	1 cut(s)	456
BspT104I	TTCGAA	1 cut(s)	200
BsrBI	CCGCTC	1 cut(s)	171
BsrI	ACTGG	4 cut(s)	511, 661, 1133, 1196
BssECI	CCNNGG	3 cut(s)	87, 1337, 1522
BssNAI	GTATAC	2 cut(s)	753, 992
BssT1I	CCWWGG	1 cut(s)	1337
Bst1107I	GTATAC	2 cut(s)	753, 992
Bst2UI	CCWGG	3 cut(s)	106, 849, 1098
Bst4CI	ACNGT	3 cut(s)	377, 989, 1039
Bst6I	CTCTTC	3 cut(s)	450, 456, 1302
BstAPI	GCANNNNNTGC	1 cut(s)	1189
BstBAI	YACGTR	2 cut(s)	746, 750
BstBI	TTCGAA	1 cut(s)	200
BstC8I	GCNNGC	1 cut(s)	782
BstDEI	CTNAG	5 cut(s)	339, 1179, 1350, 1429, 1468
BstF5I	GGATG	3 cut(s)	223, 351, 1243
BstFNI	CGCG	2 cut(s)	165, 861
BstMAI	GTCTC	1 cut(s)	392
BstMCI	CGRYCG	3 cut(s)	18, 163, 1253
BstMWI	GCNNNNNNNGC	6 cut(s)	73, 155, 177, 431, 1175, 1189
BstNI	CCWGG	3 cut(s)	106, 849, 1098
BstNSI	RCATGY	1 cut(s)	929
BstSCI	CCNGG	6 cut(s)	27, 104, 213, 847, 1096, 1521
BstSFI	CTRYAG	1 cut(s)	1280
BstSNI	TACGTA	1 cut(s)	750
BstUI	CGCG	2 cut(s)	165, 861
BstV1I	GCAGC	2 cut(s)	1375, 1402
BstX2I	RGATCY	5 cut(s)	127, 1285, 1403, 1442, 1502
BstYI	RGATCY	5 cut(s)	127, 1285, 1403, 1442, 1502
BstZ17I	GTATAC	2 cut(s)	753, 992
BstZI	CGGCCG	2 cut(s)	15, 1250
BsuI	GTATCC	1 cut(s)	863
BsuRI	GGCC	5 cut(s)	17, 180, 386, 425, 1252
BtgZI	GCGATG	1 cut(s)	876
BtrI	CACGTC	2 cut(s)	1418, 1499
BtsCI	GGATG	3 cut(s)	223, 351, 1243
BtsI	GCAGTG	2 cut(s)	139, 831
BtsIMutI	CAGTG	5 cut(s)	139, 382, 654, 673, 831
BveI	ACCTGC	1 cut(s)	250
Cac8I	GCNNGC	1 cut(s)	782
CaiI	CAGNNNCTG	1 cut(s)	1274
CciI	TCATGA	1 cut(s)	295
Cfr13I	GGNCC	5 cut(s)	43, 179, 385, 423, 1094
CseI	GACGC	1 cut(s)	154
CsiI	ACCWGGT	1 cut(s)	847
Csp6I	GTAC	4 cut(s)	747, 947, 1035, 1199
CviAII	CATG	5 cut(s)	296, 467, 926, 1013, 1457
CviQI	GTAC	4 cut(s)	747, 947, 1035, 1199
DdeI	CTNAG	5 cut(s)	339, 1179, 1350, 1429, 1468
DraI	TTTAAA	1 cut(s)	1155
DriI	GACNNNNNGTC	1 cut(s)	310
EaeI	YGGCCR	2 cut(s)	15, 1250
EagI	CGGCCG	2 cut(s)	15, 1250
Eam1104I	CTCTTC	3 cut(s)	450, 456, 1302
Eam1105I	GACNNNNNGTC	1 cut(s)	310
EarI	CTCTTC	3 cut(s)	450, 456, 1302
EciI	GGCGGA	2 cut(s)	136, 689
Ecl136II	GAGCTC	3 cut(s)	76, 338, 649
EclXI	CGGCCG	2 cut(s)	15, 1250
Eco105I	TACGTA	1 cut(s)	750
Eco130I	CCWWGG	1 cut(s)	1337
Eco24I	GRGCYC	4 cut(s)	78, 340, 651, 1476
Eco32I	GATATC	1 cut(s)	1215
Eco47I	GGWCC	2 cut(s)	43, 1094
Eco52I	CGGCCG	2 cut(s)	15, 1250
Eco53kI	GAGCTC	3 cut(s)	76, 338, 649
Eco57I	CTGAAG	1 cut(s)	649
Eco88I	CYCGRG	1 cut(s)	1476
EcoICRI	GAGCTC	3 cut(s)	76, 338, 649
EcoO109I	RGGNCCY	1 cut(s)	43
EcoRI	GAATTC	1 cut(s)	223
EcoRII	CCWGG	3 cut(s)	104, 847, 1096
EcoRV	GATATC	1 cut(s)	1215
EcoT14I	CCWWGG	1 cut(s)	1337
EcoT38I	GRGCYC	4 cut(s)	78, 340, 651, 1476
ErhI	CCWWGG	1 cut(s)	1337
FaeI	CATG	5 cut(s)	299, 470, 929, 1016, 1460
FaqI	GGGAC	2 cut(s)	341, 998
FatI	CATG	5 cut(s)	295, 466, 925, 1012, 1456
FauI	CCCGC	1 cut(s)	474
FbaI	TGATCA	1 cut(s)	900
FblI	GTMKAC	4 cut(s)	530, 752, 991, 1495
Fnu4HI	GCNGC	3 cut(s)	672, 1364, 1391
FokI	GGATG	3 cut(s)	230, 358, 1250
FriOI	GRGCYC	4 cut(s)	78, 340, 651, 1476
Fsp4HI	GCNGC	3 cut(s)	672, 1364, 1391
FspBI	CTAG	5 cut(s)	317, 540, 1323, 1338, 1552
GluI	GCNGC	3 cut(s)	672, 1364, 1391
GsuI	CTGGAG	1 cut(s)	1179
HaeIII	GGCC	5 cut(s)	17, 180, 386, 425, 1252
HapII	CCGG	4 cut(s)	28, 38, 215, 1522
HgaI	GACGC	1 cut(s)	154
Hin1II	CATG	5 cut(s)	299, 470, 929, 1016, 1460
HincII	GTYRAC	3 cut(s)	628, 1496, 1534
HindII	GTYRAC	3 cut(s)	628, 1496, 1534
HindIII	AAGCTT	1 cut(s)	707
HinfI	GANTC	5 cut(s)	81, 197, 400, 1481, 1492
HpaI	GTTAAC	1 cut(s)	628
HpaII	CCGG	4 cut(s)	28, 38, 215, 1522
HphI	GGTGA	6 cut(s)	112, 203, 296, 910, 1064, 1326
Hpy188I	TCNGA	8 cut(s)	73, 80, 91, 352, 1353, 1380, 1432, 1471
Hpy188III	TCNNGA	6 cut(s)	228, 296, 568, 713, 1268, 1506
Hpy99I	CGWCG	3 cut(s)	335, 789, 1500
HpyAV	CCTTC	4 cut(s)	974, 1103, 1155, 1521
HpyCH4III	ACNGT	3 cut(s)	377, 989, 1039
HpyCH4IV	ACGT	5 cut(s)	745, 749, 1417, 1498, 1536
HpyCH4V	TGCA	4 cut(s)	259, 824, 960, 1059
HpyF10VI	GCNNNNNNNGC	6 cut(s)	73, 155, 177, 431, 1175, 1189
HpyF3I	CTNAG	5 cut(s)	339, 1179, 1350, 1429, 1468
HpySE526I	ACGT	5 cut(s)	745, 749, 1417, 1498, 1536
Hsp92II	CATG	5 cut(s)	299, 470, 929, 1016, 1460
Ksp22I	TGATCA	1 cut(s)	900
KspAI	GTTAAC	1 cut(s)	628
LguI	GCTCTTC	1 cut(s)	456
LmnI	GCTCC	8 cut(s)	73, 81, 149, 289, 335, 544, 654, 1341
Lsp1109I	GCAGC	2 cut(s)	1375, 1402
LweI	GCATC	2 cut(s)	301, 969
MabI	ACCWGGT	1 cut(s)	847
MaeI	CTAG	5 cut(s)	317, 540, 1323, 1338, 1552
MaeII	ACGT	5 cut(s)	745, 749, 1417, 1498, 1536
MaeIII	GTNAC	2 cut(s)	312, 790
MbiI	CCGCTC	1 cut(s)	171
MboII	GAAGA	9 cut(s)	452, 464, 467, 473, 595, 655, 1242, 1280, 1319
MflI	RGATCY	5 cut(s)	127, 1285, 1403, 1442, 1502
MhlI	GDGCHC	4 cut(s)	78, 340, 651, 1476
MlyI	GAGTC	3 cut(s)	394, 1490, 1501
MmeI	TCCRAC	2 cut(s)	330, 1469
MseI	TTAA	6 cut(s)	612, 627, 737, 1082, 1154, 1328
MslI	CAYNNNNRTG	3 cut(s)	216, 297, 300
MspI	CCGG	4 cut(s)	28, 38, 215, 1522
MspR9I	CCNGG	6 cut(s)	29, 106, 215, 849, 1098, 1523
MvaI	CCWGG	3 cut(s)	106, 849, 1098
MvnI	CGCG	2 cut(s)	165, 861
MwoI	GCNNNNNNNGC	6 cut(s)	73, 155, 177, 431, 1175, 1189
NciI	CCSGG	3 cut(s)	29, 215, 1523
NlaIII	CATG	5 cut(s)	299, 470, 929, 1016, 1460
NlaIV	GGNNCC	5 cut(s)	45, 354, 387, 1343, 1520
NmeAIII	GCCGAG	1 cut(s)	156
NmuCI	GTSAC	1 cut(s)	312
NspI	RCATGY	1 cut(s)	929
NspV	TTCGAA	1 cut(s)	200
PagI	TCATGA	1 cut(s)	295
PaqCI	CACCTGC	1 cut(s)	250
PciSI	GCTCTTC	1 cut(s)	456
PcsI	WCGNNNNNNNCGW	1 cut(s)	162
PfeI	GAWTC	2 cut(s)	81, 197
Pfl23II	CGTACG	1 cut(s)	746
PflMI	CCANNNNNTGG	1 cut(s)	188
PkrI	GCNGC	3 cut(s)	673, 1365, 1392
Ple19I	CGATCG	1 cut(s)	163
PleI	GAGTC	3 cut(s)	394, 1489, 1500
PpsI	GAGTC	3 cut(s)	394, 1489, 1500
Ppu21I	YACGTR	2 cut(s)	746, 750
PpuMI	RGGWCCY	1 cut(s)	43
PshBI	ATTAAT	1 cut(s)	612
PsiI	TTATAA	1 cut(s)	705
Psp124BI	GAGCTC	3 cut(s)	78, 340, 651
Psp5II	RGGWCCY	1 cut(s)	43
Psp6I	CCWGG	3 cut(s)	104, 847, 1096
PspGI	CCWGG	3 cut(s)	104, 847, 1096
PspLI	CGTACG	1 cut(s)	746
PspN4I	GGNNCC	5 cut(s)	45, 354, 387, 1343, 1520
PspPI	GGNCC	5 cut(s)	43, 179, 385, 423, 1094
PspPPI	RGGWCCY	1 cut(s)	43
PsrI	GAACNNNNNNTAC	2 cut(s)	1263, 1295
PstNI	CAGNNNCTG	1 cut(s)	1274
PsuI	RGATCY	5 cut(s)	127, 1285, 1403, 1442, 1502
PvuI	CGATCG	1 cut(s)	163
RsaI	GTAC	4 cut(s)	748, 948, 1036, 1200
RsaNI	GTAC	4 cut(s)	747, 947, 1035, 1199
RseI	CAYNNNNRTG	3 cut(s)	216, 297, 300
SacI	GAGCTC	3 cut(s)	78, 340, 651
SalI	GTCGAC	1 cut(s)	1494
SapI	GCTCTTC	1 cut(s)	456
SaqAI	TTAA	6 cut(s)	612, 627, 737, 1082, 1154, 1328
SatI	GCNGC	3 cut(s)	672, 1364, 1391
Sau96I	GGNCC	5 cut(s)	43, 179, 385, 423, 1094
SchI	GAGTC	3 cut(s)	394, 1490, 1501
ScrFI	CCNGG	6 cut(s)	29, 106, 215, 849, 1098, 1523
SduI	GDGCHC	4 cut(s)	78, 340, 651, 1476
SexAI	ACCWGGT	1 cut(s)	847
SfaNI	GCATC	2 cut(s)	301, 969
SfcI	CTRYAG	1 cut(s)	1280
SfuI	TTCGAA	1 cut(s)	200
SinI	GGWCC	2 cut(s)	43, 1094
SmiMI	CAYNNNNRTG	3 cut(s)	216, 297, 300
SmlI	CTYRAG	1 cut(s)	644
SmoI	CTYRAG	1 cut(s)	644
SnaBI	TACGTA	1 cut(s)	750
SsiI	CCGC	5 cut(s)	147, 169, 481, 671, 674
SspMI	CTAG	5 cut(s)	317, 540, 1323, 1338, 1552
SstI	GAGCTC	3 cut(s)	78, 340, 651
StyD4I	CCNGG	6 cut(s)	27, 104, 213, 847, 1096, 1521
StyI	CCWWGG	1 cut(s)	1337
TaaI	ACNGT	3 cut(s)	377, 989, 1039
TaiI	ACGT	5 cut(s)	748, 752, 1420, 1501, 1539
TaqI	TCGA	7 cut(s)	33, 200, 412, 636, 873, 1024, 1495
TaqII	GACCGA	1 cut(s)	20
TauI	GCSGC	1 cut(s)	674
TfiI	GAWTC	2 cut(s)	81, 197
Tru1I	TTAA	6 cut(s)	612, 627, 737, 1082, 1154, 1328
Tru9I	TTAA	6 cut(s)	612, 627, 737, 1082, 1154, 1328
TscAI	CASTG	5 cut(s)	146, 382, 661, 673, 831
TseFI	GTSAC	1 cut(s)	312
TseI	GCWGC	2 cut(s)	1363, 1390
Tsp45I	GTSAC	1 cut(s)	312
TspDTI	ATGAA	3 cut(s)	993, 1104, 1254
TspGWI	ACGGA	4 cut(s)	257, 348, 1173, 1225
TspRI	CASTG	5 cut(s)	146, 382, 661, 673, 831
Van91I	CCANNNNNTGG	1 cut(s)	188
VpaK11BI	GGWCC	2 cut(s)	43, 1094
VspI	ATTAAT	1 cut(s)	612
XapI	RAATTY	3 cut(s)	223, 1330, 1540
XceI	RCATGY	1 cut(s)	929
XmaJI	CCTAGG	1 cut(s)	1337
XmiI	GTMKAC	4 cut(s)	530, 752, 991, 1495
XspI	CTAG	5 cut(s)	317, 540, 1323, 1338, 1552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.