MD02G1138400.v1.1

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0020379
Physical Location & Seq
Forward (+)
11585861 .. 11586187
327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1138400.v1.1.491

Sequence Viewer

Length: 327 bp
ATGTTTCTAAAGATGGCAAAATTCGCGGCCGCCAGAATAGTCCCCATTCACTTTCAACGAGTGCCATGCTTTAAGAGGGGAGGGATCAAGTTCTATCTCGGTGTGCGCCCTAATTTCCTGCTTGTGTTGGTAGACAACGTTGGAGGGGCTGGTGAAGTGGTAGATGTAAAAGTGAAAGGCTTGAGCACTGGTTGGATACAAATGACGCGTAACTGGGGACAACAGACTGGAGGAGGTTTGGAAGGAAAAAGTTTGTCTTTCCAAGTAACTACTAGTGATAGAAGGGTGGTGGAGTCTCTCAACGTTGTGCCTGCCAATTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

109

Amino Acids

11.92

Weight (kDa)

10.43

Isoelectric Point (pI)

22.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Expansin_C PF01357 29 - 104 1.6e-20 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020379)

Species Orthologous Gene IDs
malus_domestica MD02G1138400.v1.1
pyrus_communis pycom02g10970
rosa_chinensis RchiOBHm_Chr5g0061561
rosa_multiflora Rmu_co8111106.1_g000001 Rmu_co8391917.1_g000001 Rmu_sc0038331.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 26, 208
AciI CCGC 2 cut(s) 26, 30
AclI AACGTT 2 cut(s) 138, 303
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 20
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 1 cut(s) 56
AhlI ACTAGT 1 cut(s) 272
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 27
ApoI RAATTY 1 cut(s) 20
AspLEI GCGC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 188
BccI CCATC 1 cut(s) 7
BcgI CGANNNNNNTGC 2 cut(s) 48, 82
BciVI GTATCC 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 300
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 1 cut(s) 273
BfuI GTATCC 1 cut(s) 189
BisI GCNGC 2 cut(s) 27, 30
BlsI GCNGC 2 cut(s) 28, 31
BmrI ACTGGG 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 202
Bse1I ACTGG 3 cut(s) 193, 218, 232
BseNI ACTGG 3 cut(s) 193, 218, 232
BseRI GAGGAG 1 cut(s) 246
BseX3I CGGCCG 1 cut(s) 27
Bsh1236I CGCG 2 cut(s) 26, 208
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 188
BslFI GGGAC 2 cut(s) 26, 231
BsmAI GTCTC 1 cut(s) 300
BsmFI GGGAC 2 cut(s) 26, 231
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 84
BspACI CCGC 2 cut(s) 26, 30
BspANI GGCC 1 cut(s) 29
BspFNI CGCG 2 cut(s) 26, 208
BspPI GGATC 1 cut(s) 92
BsrI ACTGG 3 cut(s) 193, 218, 232
BssMI GATC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 312
BstFNI CGCG 2 cut(s) 26, 208
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 1 cut(s) 84
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstUI CGCG 2 cut(s) 26, 208
BstZI CGGCCG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 189
BsuRI GGCC 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 312
CciNI GCGGCCGC 1 cut(s) 27
CfoI GCGC 1 cut(s) 108
CseI GACGC 1 cut(s) 214
CviAII CATG 1 cut(s) 66
CviJI RGCY 3 cut(s) 29, 149, 180
CviKI_1 RGCY 3 cut(s) 29, 149, 180
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 27
Eco52I CGGCCG 1 cut(s) 27
FaeI CATG 1 cut(s) 69
FaiI YATR 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 241, 273
FaqI GGGAC 2 cut(s) 26, 231
FatI CATG 1 cut(s) 65
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 27, 30
Fsp4HI GCNGC 2 cut(s) 27, 30
FspBI CTAG 1 cut(s) 273
GlaI GCGC 1 cut(s) 107
GluI GCNGC 2 cut(s) 27, 30
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 1 cut(s) 29
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 108
Hin1II CATG 1 cut(s) 69
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HinfI GANTC 1 cut(s) 293
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 2 cut(s) 236, 276
HpyCH4IV ACGT 2 cut(s) 138, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 138, 303
Hsp92II CATG 1 cut(s) 69
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 1 cut(s) 84
LpnPI CCDG 6 cut(s) 46, 131, 135, 174, 199, 213
MaeI CTAG 1 cut(s) 273
MaeII ACGT 2 cut(s) 138, 303
MaeIII GTNAC 2 cut(s) 209, 265
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MfeI CAATTG 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 3 cut(s) 20, 112, 316
MluI ACGCGT 1 cut(s) 206
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 2 cut(s) 121, 173
MnlI CCTC 5 cut(s) 69, 74, 137, 224, 227
MseI TTAA 1 cut(s) 72
MunI CAATTG 1 cut(s) 316
MvnI CGCG 2 cut(s) 26, 208
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 1 cut(s) 84
NlaIII CATG 1 cut(s) 69
NotI GCGGCCGC 1 cut(s) 27
PkrI GCNGC 2 cut(s) 28, 31
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Psp1406I AACGTT 2 cut(s) 138, 303
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 2 cut(s) 27, 30
Sau3AI GATC 1 cut(s) 84
SchI GAGTC 1 cut(s) 302
SduI GDGCHC 1 cut(s) 188
SetI ASST 3 cut(s) 141, 238, 306
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 3 cut(s) 20, 112, 316
SsiI CCGC 2 cut(s) 26, 30
SspMI CTAG 1 cut(s) 273
TaiI ACGT 2 cut(s) 141, 306
TasI AATT 3 cut(s) 20, 112, 316
TauI GCSGC 2 cut(s) 29, 32
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 193
TspRI CASTG 1 cut(s) 193
XapI RAATTY 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.