MD02G1171100.v1.1

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017222
Physical Location & Seq
Reverse (-)
14833590 .. 14834210
621 bp
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UTR
Exon/CDS
Intron
MD02G1171100.v1.1.491

Sequence Viewer

Length: 621 bp
ATGCAGTATTCATCGCATTATAATTTAATACCATCTGAAGAAAAAACAATGTCAAATTTCAGCTTTTTCGGGTTTCAATATGGCACTCCAAGAAAGCCTCCAAACAAACCAGCTCCCCAAATGAGTAATATCTCCAAAGAAAGATCGAAAAGGTATAGTTTTTCAAGGCCTTCATCCCAAACAAAAGTGGAGGAAGTAAGGTACGTGTTCGATAAATTCGACACGAACAAAGACGGTAGAATCAGCAGGGAGGAGTACAAGTCGGCTCTCAAGACCCTGGACAAAGACATTGCAGAAGGTGAGATTGCCAAGACATTCAAGGCCCTTGACTCTGATGGGGATGGCTTCGTTGGGTTCACGGAGTTTGTGGAAATGTTCAACATGGGAAATGATGAGGCGAGAAAGGCGGACATCGAAAGTGCGTTTCGAGTGTTCGATTTGGATGGGAATGGGAAAATAAGTGCAGAGGAGTTGTCCTTGGTGTTGAAGAAGTTGGGAGAGAATTGCAGCCTTGGGGCTTGTAGGAATATGGTGAAAGGTGTGGACTCTGATGGTGATGGTTTAATTGACATGAATGAGTTTACCAATATGATGGGAGGCTTCAAAACACCAACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

207

Amino Acids

23.11

Weight (kDa)

5.27

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 60 - 90 4e-07 EF-hand domain pair
EF-hand_7 PF13499 64 - 126 1.2e-14 EF-hand domain pair
EF-hand_1 PF00036 65 - 93 9.7e-08 EF hand domain
EF-hand_6 PF13405 65 - 93 2.4e-07 EF-hand domain
EF-hand_5 PF13202 69 - 88 4.2e-07 EF hand
EF-hand_1 PF00036 101 - 127 6.2e-06 EF hand domain
EF-hand_7 PF13499 136 - 198 9.2e-11 EF-hand domain pair
EF-hand_1 PF00036 137 - 165 1.6e-07 EF hand domain
EF-hand_6 PF13405 138 - 166 2.8e-07 EF-hand domain
EF-hand_5 PF13202 138 - 160 4.6e-07 EF hand
EF-hand_8 PF13833 151 - 198 1.3e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15930
malus_domestica MD02G1171100.v1.1 MD15G1282600.v1.1
prunus_persica Prupe.7G134500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105321
rosa_multiflora Rmu_sc0001407.1_g000015
rosa_roxburghii Rroxscaffold_2G00138290
rosa_rugosa Rorug02G0127600
rosa_samantha Rh2AG178200 Rh2BG186600 Rh2CG182800 Rh2DG184200
rosa_wichuraiana Rw2G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
AciI CCGC 1 cut(s) 407
AcsI RAATTY 2 cut(s) 55, 215
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 2 cut(s) 203, 257
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 6 cut(s) 77, 165, 319, 379, 487, 604
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 2 cut(s) 63, 113
AluI AGCT 2 cut(s) 63, 113
AoxI GGCC 2 cut(s) 167, 321
ApeKI GCWGC 1 cut(s) 507
ApoI RAATTY 2 cut(s) 55, 215
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 3 cut(s) 311, 544, 566
BbvI GCAGC 1 cut(s) 519
BccI CCATC 7 cut(s) 40, 329, 335, 437, 545, 551, 586
BciT130I CCWGG 1 cut(s) 278
BisI GCNGC 1 cut(s) 508
BlsI GCNGC 1 cut(s) 509
Bme1390I CCNGG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 205
BsaJI CCNNGG 3 cut(s) 276, 477, 511
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 276, 477, 511
BseGI GGATG 3 cut(s) 173, 346, 448
BseMI GCAATG 1 cut(s) 288
BseRI GAGGAG 2 cut(s) 266, 482
BseXI GCAGC 1 cut(s) 519
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 2 cut(s) 169, 323
BsnI GGCC 2 cut(s) 169, 323
Bsp143I GATC 1 cut(s) 143
BspACI CCGC 1 cut(s) 407
BspANI GGCC 2 cut(s) 169, 323
BsrDI GCAATG 1 cut(s) 288
BssECI CCNNGG 3 cut(s) 276, 477, 511
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 477, 511
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 236
BstBAI YACGTR 1 cut(s) 205
BstF5I GGATG 3 cut(s) 173, 346, 448
BstKTI GATC 1 cut(s) 146
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 592
BsuRI GGCC 2 cut(s) 169, 323
BtsCI GGATG 3 cut(s) 173, 346, 448
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 202, 256
CviAII CATG 2 cut(s) 382, 571
CviQI GTAC 2 cut(s) 202, 256
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 422
Eco130I CCWWGG 2 cut(s) 477, 511
Eco147I AGGCCT 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 2 cut(s) 477, 511
ErhI CCWWGG 2 cut(s) 477, 511
FaeI CATG 2 cut(s) 385, 574
FaiI YATR 7 cut(s) 21, 81, 156, 383, 530, 572, 590
FatI CATG 2 cut(s) 381, 570
Fnu4HI GCNGC 1 cut(s) 508
FokI GGATG 3 cut(s) 160, 353, 455
Fsp4HI GCNGC 1 cut(s) 508
GluI GCNGC 1 cut(s) 508
HaeIII GGCC 2 cut(s) 169, 323
Hin1II CATG 2 cut(s) 385, 574
HinfI GANTC 3 cut(s) 240, 329, 545
HphI GGTGA 3 cut(s) 311, 544, 566
Hpy166II GTNNAC 3 cut(s) 357, 544, 582
Hpy188I TCNGA 3 cut(s) 37, 334, 550
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 3 cut(s) 357, 544, 582
HpyAV CCTTC 2 cut(s) 180, 290
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 4 cut(s) 4, 293, 464, 507
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 2 cut(s) 385, 574
Kzo9I GATC 1 cut(s) 143
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 4 cut(s) 123, 232, 263, 290
Lsp1109I GCAGC 1 cut(s) 519
MaeII ACGT 1 cut(s) 204
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 2 cut(s) 50, 499
MluCI AATT 5 cut(s) 22, 55, 215, 502, 564
MlyI GAGTC 2 cut(s) 323, 539
MnlI CCTC 6 cut(s) 108, 184, 244, 388, 460, 590
MseI TTAA 3 cut(s) 26, 563, 619
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeII GATC 1 cut(s) 143
NlaIII CATG 2 cut(s) 385, 574
PceI AGGCCT 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 1 cut(s) 240
PkrI GCNGC 1 cut(s) 509
PleI GAGTC 2 cut(s) 323, 539
PpsI GAGTC 2 cut(s) 323, 539
Ppu21I YACGTR 1 cut(s) 205
PsiI TTATAA 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspPI GGNCC 1 cut(s) 322
RsaI GTAC 2 cut(s) 203, 257
RsaNI GTAC 2 cut(s) 202, 256
SaqAI TTAA 3 cut(s) 26, 563, 619
SatI GCNGC 1 cut(s) 508
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 2 cut(s) 323, 539
ScrFI CCNGG 1 cut(s) 278
SetI ASST 7 cut(s) 65, 115, 155, 203, 207, 301, 541
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 5 cut(s) 22, 55, 215, 502, 564
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 1 cut(s) 407
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 2 cut(s) 477, 511
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 1 cut(s) 207
TaqI TCGA 6 cut(s) 146, 210, 219, 414, 427, 435
TasI AATT 5 cut(s) 22, 55, 215, 502, 564
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 3 cut(s) 26, 563, 619
Tru9I TTAA 3 cut(s) 26, 563, 619
TseI GCWGC 1 cut(s) 507
TspDTI ATGAA 2 cut(s) 162, 587
TspGWI ACGGA 1 cut(s) 374
XapI RAATTY 2 cut(s) 55, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.