Rh2DG184200

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017222
Physical Location & Seq
Reverse (-)
16344298 .. 16345662
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG184200.1

Sequence Viewer

Length: 579 bp
ATGTCACATTTCAGCTTTCTAGGGTTTCAGTATGGCTCATTCACACTGAGAAAGTCTCGCAATAAACCAGCGCCCAAATTGAGTACTAATGTCTCCAAAGAGAGACAGAGTTCTAGCTCGTCAAGGTCTTCATCCCAGCCAAAAATGGAGGAGATAAGATGGGTGTTTGATAAATTTGACACCAACAAAGACGGGAAGATAAGTCGGGAGGAGTACAAGTCGGCGTTGAAAACCCTCGACAAAGAAATGACAGAAGCTGAGATTGCCAAGACATTCAAGGCCCTTGATTCTGACGGAGACGGGTCCATAGGCTGGACGGAGTTTGTGGAGATGTTCAACATGGGAGCTGATGAGGCCAAGAAAGCTGACATTGAAGGCGCTTTTCGGGTGTTTGATTTGGATGGAAATGGGAAGATAAGCGCAGAGGAGTTGTCTCTTGTTCTCAAGAAGCTGGGAGAGAATTGCAGCCTTGGGGCTTGTAAGAAAATGGTGAAAGGGGTGGATGTTGATGGTGATGGTTTGATAGATATAAATGAGTTTACCAAAATGATGGGAAGCTTCAAGAAACCAAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

192

Amino Acids

21.33

Weight (kDa)

6.64

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARC_Ca_bdg PF10591 31 - 110 9.8e-07 Secreted protein acidic and rich in cysteine Ca binding region
EF-hand_7 PF13499 50 - 112 4.7e-18 EF-hand domain pair
EF-hand_1 PF00036 51 - 79 1.2e-08 EF hand domain
EF-hand_5 PF13202 55 - 74 2.1e-07 EF hand
EF-hand_6 PF13405 55 - 79 2.8e-06 EF-hand domain
EF-hand_8 PF13833 74 - 114 4.6e-07 EF-hand domain pair
EF-hand_7 PF13499 122 - 184 1.2e-11 EF-hand domain pair
EF-hand_1 PF00036 124 - 151 4.1e-07 EF hand domain
EF-hand_6 PF13405 124 - 152 1e-06 EF-hand domain
EF-hand_5 PF13202 127 - 146 9e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15930
malus_domestica MD02G1171100.v1.1 MD15G1282600.v1.1
prunus_persica Prupe.7G134500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105321
rosa_multiflora Rmu_sc0001407.1_g000015
rosa_roxburghii Rroxscaffold_2G00138290
rosa_rugosa Rorug02G0127600
rosa_samantha Rh2AG178200 Rh2BG186600 Rh2CG182800 Rh2DG184200
rosa_wichuraiana Rw2G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 312
AcsI RAATTY 1 cut(s) 173
AfaI GTAC 3 cut(s) 85, 215, 574
AfiI CCNNNNNNNGG 1 cut(s) 312
AgsI TTSAA 5 cut(s) 229, 277, 337, 374, 562
AluBI AGCT 7 cut(s) 15, 117, 257, 347, 365, 451, 558
AluI AGCT 7 cut(s) 15, 117, 257, 347, 365, 451, 558
Alw26I GTCTC 5 cut(s) 60, 97, 97, 291, 438
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 2 cut(s) 279, 354
ApeKI GCWGC 1 cut(s) 465
ApoI RAATTY 1 cut(s) 173
AspLEI GCGC 3 cut(s) 73, 380, 422
AspS9I GGNCC 2 cut(s) 280, 303
AsuHPI GGTGA 2 cut(s) 502, 524
AvaII GGWCC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 477
BccI CCATC 5 cut(s) 153, 395, 503, 509, 544
BcoDI GTCTC 5 cut(s) 60, 97, 97, 291, 438
BfaI CTAG 2 cut(s) 20, 114
BfoI RGCGCY 2 cut(s) 74, 381
BisI GCNGC 1 cut(s) 466
BlsI GCNGC 1 cut(s) 467
BmcAI AGTACT 2 cut(s) 85, 574
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 280, 303
BmiI GGNNCC 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 40, 72
BpuEI CTTGAG 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 3 cut(s) 131, 406, 508
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 38, 249
BseRI GAGGAG 3 cut(s) 164, 224, 440
BseXI GCAGC 1 cut(s) 477
BseYI CCCAGC 2 cut(s) 135, 451
BshFI GGCC 2 cut(s) 281, 356
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 5 cut(s) 60, 97, 97, 291, 438
BsmBI CGTCTC 1 cut(s) 291
BsnI GGCC 2 cut(s) 281, 356
BspANI GGCC 2 cut(s) 281, 356
BspCNI CTCAG 2 cut(s) 39, 250
BspLI GGNNCC 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 469
BstDEI CTNAG 2 cut(s) 47, 258
BstF5I GGATG 3 cut(s) 131, 406, 508
BstH2I RGCGCY 2 cut(s) 74, 381
BstHHI GCGC 3 cut(s) 73, 380, 422
BstMAI GTCTC 5 cut(s) 60, 97, 97, 291, 438
BstMWI GCNNNNNNNGC 3 cut(s) 263, 353, 362
BstV1I GCAGC 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 550
BsuRI GGCC 2 cut(s) 281, 356
BtsCI GGATG 3 cut(s) 131, 406, 508
BtsIMutI CAGTG 1 cut(s) 44
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 3 cut(s) 73, 380, 422
Cfr13I GGNCC 2 cut(s) 280, 303
Csp6I GTAC 3 cut(s) 84, 214, 573
CviAII CATG 1 cut(s) 340
CviQI GTAC 3 cut(s) 84, 214, 573
DdeI CTNAG 2 cut(s) 47, 258
Eco130I CCWWGG 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 469
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 1 cut(s) 343
FaiI YATR 4 cut(s) 33, 308, 341, 530
FatI CATG 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 466
FokI GGATG 3 cut(s) 118, 413, 515
Fsp4HI GCNGC 1 cut(s) 466
FspBI CTAG 2 cut(s) 20, 114
GlaI GCGC 3 cut(s) 72, 379, 421
GluI GCNGC 1 cut(s) 466
GsaI CCCAGC 2 cut(s) 139, 455
HaeII RGCGCY 2 cut(s) 74, 381
HaeIII GGCC 2 cut(s) 281, 356
HhaI GCGC 3 cut(s) 73, 380, 422
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 3 cut(s) 71, 378, 420
HinP1I GCGC 3 cut(s) 71, 378, 420
HindIII AAGCTT 1 cut(s) 556
HinfI GANTC 1 cut(s) 287
HphI GGTGA 2 cut(s) 502, 524
Hpy166II GTNNAC 1 cut(s) 540
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 3 cut(s) 206, 445, 562
Hpy8I GTNNAC 1 cut(s) 540
HpyAV CCTTC 1 cut(s) 368
HpyCH4V TGCA 1 cut(s) 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 353, 362
HpyF3I CTNAG 2 cut(s) 47, 258
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 3 cut(s) 71, 378, 420
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 4 cut(s) 81, 149, 298, 437
Lsp1109I GCAGC 1 cut(s) 477
MaeI CTAG 2 cut(s) 20, 114
MaeIII GTNAC 1 cut(s) 3
MboII GAAGA 3 cut(s) 120, 208, 424
MluCI AATT 3 cut(s) 77, 173, 460
MnlI CCTC 5 cut(s) 142, 202, 245, 346, 418
MwoI GCNNNNNNNGC 3 cut(s) 263, 353, 362
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 3
PfeI GAWTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 312
PkrI GCNGC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 135, 451
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 2 cut(s) 280, 303
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 85, 215, 574
RsaNI GTAC 3 cut(s) 84, 214, 573
SatI GCNGC 1 cut(s) 466
Sau96I GGNCC 2 cut(s) 280, 303
ScaI AGTACT 2 cut(s) 85, 574
SetI ASST 8 cut(s) 17, 119, 128, 259, 349, 367, 453, 560
SinI GGWCC 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 3 cut(s) 77, 173, 460
SspMI CTAG 2 cut(s) 20, 114
StyI CCWWGG 1 cut(s) 469
TaqI TCGA 1 cut(s) 237
TasI AATT 3 cut(s) 77, 173, 460
TatI WGTACW 3 cut(s) 83, 213, 572
TfiI GAWTC 1 cut(s) 287
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 465
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 2 cut(s) 309, 332
TspRI CASTG 1 cut(s) 51
Van91I CCANNNNNTGG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 1 cut(s) 173
XspI CTAG 2 cut(s) 20, 114
ZrmI AGTACT 2 cut(s) 85, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.