MD15G1282600.v1.1

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017222
Physical Location & Seq
Reverse (-)
25522723 .. 25523322
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1282600.v1.1.491

Sequence Viewer

Length: 600 bp
ATGTCAAATTTCAGCTTTTTCAGGTTTCAATATGGCACTCCAAGAAAGCCTCCAAACAAACCAGCTCCTCAAATGAGTAATATCTCCAAAGAAACAACCCAAGTGAGCTTCTCCAAAGAAAGGTTTGAAAGGTCTCGTTTTTCAAGGCCTTCATCCCAACCAAAAGTGGGGGAAGTAAGATATGTGTTCGATAAATTCGACATGAACAAAGATGGTAGAATCAGCAGGGAGGAGTACAAGTCGGCTCTCAAGACCCTGGACAAAGACATTGCAGAGGGTGAGATTGCCAAGACATTCAAGGCCCTTGACTCTGATGGGGATGGCTTCATTGGGTTCACCGAGTTTGTGGAAACGTTGAACATGGGAAATGATGAGGCGAGAAAGGCGGACATCGAAAGCGCGTTTCGAGTGTTCGATTTGGATGGGAATGGGAAAATAAGTGCAGAGGAGTTGTCTCTGGTGTTGAAGAAGTTGGGAGAGAATTGCAGCCTTGGGGCTTGTAGGAATATGGTGAAAGCTGTGGACTCTGATGGTGACGGTTTGATTGACATGAATGAGTTTACCAAAATGATGGGAAGCTTCAAAACACCAACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

200

Amino Acids

22.36

Weight (kDa)

5.76

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 58 - 86 3.9e-07 EF hand domain
EF-hand_6 PF13405 58 - 86 2.8e-06 EF-hand domain
EF-hand_7 PF13499 59 - 117 2.5e-13 EF-hand domain pair
EF-hand_5 PF13202 62 - 81 5.1e-07 EF hand
EF-hand_1 PF00036 94 - 120 8.9e-06 EF hand domain
EF-hand_7 PF13499 129 - 191 8.1e-12 EF-hand domain pair
EF-hand_1 PF00036 130 - 158 1.5e-07 EF hand domain
EF-hand_5 PF13202 131 - 153 4.4e-07 EF hand
EF-hand_6 PF13405 131 - 159 2.7e-07 EF-hand domain
EF-hand_8 PF13833 143 - 191 3.4e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15930
malus_domestica MD02G1171100.v1.1 MD15G1282600.v1.1
prunus_persica Prupe.7G134500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105321
rosa_multiflora Rmu_sc0001407.1_g000015
rosa_roxburghii Rroxscaffold_2G00138290
rosa_rugosa Rorug02G0127600
rosa_samantha Rh2AG178200 Rh2BG186600 Rh2CG182800 Rh2DG184200
rosa_wichuraiana Rw2G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 401
AciI CCGC 1 cut(s) 386
AclI AACGTT 1 cut(s) 353
AcsI RAATTY 2 cut(s) 7, 194
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 120, 167
AgsI TTSAA 7 cut(s) 29, 128, 144, 298, 358, 466, 583
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 5 cut(s) 15, 65, 108, 518, 579
AluI AGCT 5 cut(s) 15, 65, 108, 518, 579
Alw26I GTCTC 2 cut(s) 138, 459
AoxI GGCC 2 cut(s) 146, 300
ApeKI GCWGC 1 cut(s) 486
ApoI RAATTY 2 cut(s) 7, 194
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 4 cut(s) 290, 328, 523, 545
BbvI GCAGC 1 cut(s) 498
BccI CCATC 6 cut(s) 206, 308, 314, 416, 524, 565
BciT130I CCWGG 1 cut(s) 257
BcoDI GTCTC 2 cut(s) 138, 459
BisI GCNGC 1 cut(s) 487
BlsI GCNGC 1 cut(s) 488
Bme1390I CCNGG 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 2 cut(s) 255, 490
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 167
Bse3DI GCAATG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 2 cut(s) 255, 490
BseGI GGATG 3 cut(s) 152, 325, 427
BseLI CCNNNNNNNGG 2 cut(s) 120, 167
BseMI GCAATG 1 cut(s) 267
BseRI GAGGAG 3 cut(s) 57, 245, 461
BseXI GCAGC 1 cut(s) 498
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 2 cut(s) 148, 302
BslI CCNNNNNNNGG 2 cut(s) 120, 167
BsmAI GTCTC 2 cut(s) 138, 459
BsnI GGCC 2 cut(s) 148, 302
Bso31I GGTCTC 1 cut(s) 138
BspACI CCGC 1 cut(s) 386
BspANI GGCC 2 cut(s) 148, 302
BspFNI CGCG 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 255, 490
BssT1I CCWWGG 1 cut(s) 490
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 2 cut(s) 539, 595
BstF5I GGATG 3 cut(s) 152, 325, 427
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 1 cut(s) 401
BstMAI GTCTC 2 cut(s) 138, 459
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 255
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 498
BstXI CCANNNNNNTGG 1 cut(s) 571
BsuRI GGCC 2 cut(s) 148, 302
BtsCI GGATG 3 cut(s) 152, 325, 427
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 301
Csp6I GTAC 1 cut(s) 235
CviAII CATG 3 cut(s) 202, 361, 550
CviQI GTAC 1 cut(s) 235
EciI GGCGGA 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 490
Eco147I AGGCCT 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 3 cut(s) 205, 364, 553
FaiI YATR 6 cut(s) 33, 183, 203, 362, 509, 551
FatI CATG 3 cut(s) 201, 360, 549
Fnu4HI GCNGC 1 cut(s) 487
FokI GGATG 3 cut(s) 139, 332, 434
Fsp4HI GCNGC 1 cut(s) 487
GlaI GCGC 1 cut(s) 400
GluI GCNGC 1 cut(s) 487
HaeIII GGCC 2 cut(s) 148, 302
HhaI GCGC 1 cut(s) 401
Hin1II CATG 3 cut(s) 205, 364, 553
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 577
HinfI GANTC 3 cut(s) 219, 308, 524
HphI GGTGA 4 cut(s) 290, 328, 523, 545
Hpy166II GTNNAC 3 cut(s) 336, 523, 561
Hpy188I TCNGA 2 cut(s) 313, 529
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 3 cut(s) 336, 523, 561
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 2 cut(s) 539, 595
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 3 cut(s) 272, 443, 486
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 3 cut(s) 205, 364, 553
HspAI GCGC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 6 cut(s) 7, 75, 211, 242, 269, 443
Lsp1109I GCAGC 1 cut(s) 498
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 533
MboII GAAGA 1 cut(s) 478
MluCI AATT 3 cut(s) 7, 194, 481
MlyI GAGTC 2 cut(s) 302, 518
MnlI CCTC 6 cut(s) 60, 78, 223, 268, 367, 439
MseI TTAA 1 cut(s) 598
MspR9I CCNGG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 1 cut(s) 383
NlaIII CATG 3 cut(s) 205, 364, 553
NmuCI GTSAC 1 cut(s) 533
PceI AGGCCT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 219
PkrI GCNGC 1 cut(s) 488
PleI GAGTC 2 cut(s) 302, 518
PpsI GAGTC 2 cut(s) 302, 518
Psp1406I AACGTT 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspPI GGNCC 1 cut(s) 301
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 598
SatI GCNGC 1 cut(s) 487
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 2 cut(s) 302, 518
ScrFI CCNGG 1 cut(s) 257
SetI ASST 9 cut(s) 17, 26, 67, 110, 125, 134, 356, 520, 581
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 7, 194, 481
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 1 cut(s) 386
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 2 cut(s) 539, 595
TaiI ACGT 1 cut(s) 356
TaqI TCGA 5 cut(s) 189, 198, 393, 406, 414
TasI AATT 3 cut(s) 7, 194, 481
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 598
Tru9I TTAA 1 cut(s) 598
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 1 cut(s) 486
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 4 cut(s) 141, 218, 316, 566
XapI RAATTY 2 cut(s) 7, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.