Rorug02G0127600

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017222
Physical Location & Seq
Forward (+)
10979699 .. 10980342
644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0127600.1

Sequence Viewer

Length: 558 bp
ATGTCTGCCACCGAAGAAGTCGAAGCTCCGACCGTGGAGGCCCCGCCGGCGACGGAGGAGAAGCCGGTGAGGGAGAAAAAGCTCAGGGATCTGAAGGAGAAGAAGCCCAAACAGCCCAAAACCGCTGCTCATCCTCCCTATTTTCAGATGATCACAGAGGCACTGTTGGCTCTGAACGAGAAGAGCGGGTCGAGTCCTTACGCCATAGCCAAGTACATGGAGGAGAAGCACGAGGCGGTGCTTCCGGCCAATTTCAAGAAGACTCTGGCTCTGCAATTGAAGAACTCGGAGGCGAGAGGGAAACTGGTCAAGGTCAGGGCCTCTTACAAGTTGTCTGAGGCGGGGAAGGAGGACAAGAGCGCAAGCAAAAGAACAACTCGAGCTTCGAAGCCCAAGGAGGAGGAGAAGAAGAATACTAAGACTAAGGCGTCTGCTGCTCCAAAGGTGACGAAGAAGAAGCCGGCTAAGAAGAATAAGAAATCGAGCGCTGCAAAGCCGAAGCAGCCCAAGTCGATCAAGAGCCCTGCTGCTAAGAAACCCAAGAAGGCTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

185

Amino Acids

20.3

Weight (kDa)

10.11

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 44 - 110 1.1e-15 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15930
malus_domestica MD02G1171100.v1.1 MD15G1282600.v1.1
prunus_persica Prupe.7G134500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105321
rosa_multiflora Rmu_sc0001407.1_g000015
rosa_roxburghii Rroxscaffold_2G00138290
rosa_rugosa Rorug02G0127600
rosa_samantha Rh2AG178200 Rh2BG186600 Rh2CG182800 Rh2DG184200
rosa_wichuraiana Rw2G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 186
AciI CCGC 5 cut(s) 44, 123, 186, 236, 341
AclWI GGATC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 1 cut(s) 215
AfeI AGCGCT 1 cut(s) 487
AgsI TTSAA 2 cut(s) 256, 280
AluBI AGCT 3 cut(s) 26, 82, 383
AluI AGCT 3 cut(s) 26, 82, 383
AlwI GGATC 1 cut(s) 96
Ama87I CYCGRG 1 cut(s) 378
Aor51HI AGCGCT 1 cut(s) 487
AoxI GGCC 3 cut(s) 39, 246, 318
ApeKI GCWGC 7 cut(s) 125, 434, 488, 502, 527, 548, 551
AspLEI GCGC 2 cut(s) 362, 488
AspS9I GGNCC 2 cut(s) 40, 318
AsuHPI GGTGA 2 cut(s) 79, 457
AsuII TTCGAA 1 cut(s) 386
AvaI CYCGRG 1 cut(s) 378
BanII GRGCYC 1 cut(s) 524
BauI CACGAG 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 7 cut(s) 112, 421, 475, 514, 514, 535, 538
BclI TGATCA 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 489
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 7 cut(s) 126, 435, 489, 503, 528, 549, 552
BlsI GCNGC 7 cut(s) 127, 436, 490, 504, 529, 550, 553
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 2 cut(s) 40, 318
BmiI GGNNCC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 266
Bpu10I CCTNAGC 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 33, 393
Bse118I RCCGGY 3 cut(s) 46, 64, 460
Bse1I ACTGG 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 33, 393
BseGI GGATG 1 cut(s) 130
BseMII CTCAG 2 cut(s) 97, 327
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 4 cut(s) 71, 236, 413, 416
BseXI GCAGC 7 cut(s) 112, 421, 475, 514, 514, 535, 538
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 41, 248, 320
BsiEI CGRYCG 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 4 cut(s) 47, 65, 245, 461
BsnI GGCC 3 cut(s) 41, 248, 320
BsoBI CYCGRG 1 cut(s) 378
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 524
Bsp143I GATC 3 cut(s) 88, 150, 513
BspACI CCGC 5 cut(s) 44, 123, 186, 236, 341
BspANI GGCC 3 cut(s) 41, 248, 320
BspCNI CTCAG 2 cut(s) 96, 328
BspLI GGNNCC 1 cut(s) 42
BspPI GGATC 1 cut(s) 96
BspQI GCTCTTC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 3 cut(s) 46, 64, 460
BsrI ACTGG 1 cut(s) 309
BssAI RCCGGY 3 cut(s) 46, 64, 460
BssECI CCNNGG 2 cut(s) 33, 393
BssMI GATC 3 cut(s) 88, 150, 513
BssNI GRCGYC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 34, 165
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 1 cut(s) 428
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 48, 364, 462
BstDEI CTNAG 6 cut(s) 83, 336, 417, 423, 465, 531
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 130
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 2 cut(s) 362, 488
BstKTI GATC 3 cut(s) 91, 153, 516
BstMBI GATC 3 cut(s) 88, 150, 513
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 5 cut(s) 47, 112, 167, 434, 502
BstV1I GCAGC 7 cut(s) 112, 421, 475, 514, 514, 535, 538
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 3 cut(s) 41, 248, 320
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 130
BtsIMutI CAGTG 1 cut(s) 161
Cac8I GCNNGC 3 cut(s) 48, 364, 462
CfoI GCGC 2 cut(s) 362, 488
Cfr10I RCCGGY 3 cut(s) 46, 64, 460
Cfr13I GGNCC 2 cut(s) 40, 318
CseI GACGC 1 cut(s) 417
Csp6I GTAC 1 cut(s) 214
CviAII CATG 1 cut(s) 217
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 6 cut(s) 83, 336, 417, 423, 465, 531
DpnI GATC 3 cut(s) 90, 152, 515
DpnII GATC 3 cut(s) 88, 150, 513
DrdI GACNNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 524
Eco47III AGCGCT 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 378
EcoO109I RGGNCCY 2 cut(s) 40, 318
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 1 cut(s) 220
FaiI YATR 2 cut(s) 206, 218
FatI CATG 1 cut(s) 216
FauI CCCGC 3 cut(s) 51, 179, 334
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 7 cut(s) 126, 435, 489, 503, 528, 549, 552
FokI GGATG 1 cut(s) 117
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 7 cut(s) 126, 435, 489, 503, 528, 549, 552
GlaI GCGC 2 cut(s) 361, 487
GluI GCNGC 7 cut(s) 126, 435, 489, 503, 528, 549, 552
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 3 cut(s) 41, 248, 320
HapII CCGG 4 cut(s) 47, 65, 245, 461
HgaI GACGC 1 cut(s) 417
HhaI GCGC 2 cut(s) 362, 488
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 2 cut(s) 360, 486
HinP1I GCGC 2 cut(s) 360, 486
HinfI GANTC 2 cut(s) 193, 262
HpaII CCGG 4 cut(s) 47, 65, 245, 461
HphI GGTGA 2 cut(s) 79, 457
Hpy188I TCNGA 6 cut(s) 30, 93, 147, 174, 289, 337
Hpy188III TCNNGA 2 cut(s) 256, 517
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 3 cut(s) 88, 340, 538
HpyCH4III ACNGT 2 cut(s) 34, 165
HpyCH4V TGCA 2 cut(s) 274, 491
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 112, 167, 434, 502
HpyF3I CTNAG 6 cut(s) 83, 336, 417, 423, 465, 531
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 2 cut(s) 360, 486
KroI GCCGGC 2 cut(s) 46, 460
KroNI GCCGGC 2 cut(s) 48, 462
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 3 cut(s) 88, 150, 513
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 2 cut(s) 31, 442
LpnPI CCDG 9 cut(s) 60, 70, 78, 251, 258, 290, 301, 474, 537
Lsp1109I GCAGC 7 cut(s) 112, 421, 475, 514, 514, 535, 538
MaeIII GTNAC 1 cut(s) 445
MalI GATC 3 cut(s) 90, 152, 515
MbiI CCGCTC 1 cut(s) 186
MboI GATC 3 cut(s) 88, 150, 513
MfeI CAATTG 1 cut(s) 275
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 2 cut(s) 250, 275
MlyI GAGTC 2 cut(s) 202, 256
MmeI TCCRAC 1 cut(s) 53
MreI CGCCGGCG 1 cut(s) 46
MroNI GCCGGC 2 cut(s) 46, 460
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 4 cut(s) 47, 65, 245, 461
MunI CAATTG 1 cut(s) 275
MwoI GCNNNNNNNGC 5 cut(s) 47, 112, 167, 434, 502
NaeI GCCGGC 2 cut(s) 48, 462
NdeII GATC 3 cut(s) 88, 150, 513
NgoMIV GCCGGC 2 cut(s) 46, 460
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 445
NspV TTCGAA 1 cut(s) 386
PaeR7I CTCGAG 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 176
PdiI GCCGGC 2 cut(s) 48, 462
PkrI GCNGC 7 cut(s) 127, 436, 490, 504, 529, 550, 553
PleI GAGTC 2 cut(s) 201, 256
PpsI GAGTC 2 cut(s) 201, 256
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 2 cut(s) 40, 318
PspXI VCTCGAGB 1 cut(s) 378
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 176
SatI GCNGC 7 cut(s) 126, 435, 489, 503, 528, 549, 552
Sau3AI GATC 3 cut(s) 88, 150, 513
Sau96I GGNCC 2 cut(s) 40, 318
SchI GAGTC 2 cut(s) 202, 256
SduI GDGCHC 1 cut(s) 524
SetI ASST 5 cut(s) 28, 84, 315, 385, 447
Sfr274I CTCGAG 1 cut(s) 378
SfuI TTCGAA 1 cut(s) 386
SgrAI CRCCGGYG 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
Sse9I AATT 2 cut(s) 250, 275
SsiI CCGC 5 cut(s) 44, 123, 186, 236, 341
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 34, 165
TaqI TCGA 6 cut(s) 21, 191, 379, 386, 482, 512
TasI AATT 2 cut(s) 250, 275
TatI WGTACW 1 cut(s) 213
TscAI CASTG 1 cut(s) 168
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 7 cut(s) 125, 434, 488, 502, 527, 548, 551
Tsp45I GTSAC 1 cut(s) 445
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 1 cut(s) 168
XhoI CTCGAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.