Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0018279

Physical Location & Seq

Genomic Coordinates Reverse (-)

18929017 .. 18931876

Gene Structure 2860 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1196800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGCTACAACAATAACCGCTTTAATGGAAGAACAGACATACATAGTTCACATGGACAAGGCGAAAATCACGGACTCAGATCACCAACGGTACTACAAATCTGTGATCGATTCTATCACCGCACTCTCATCTCAAGCAGAAGAGGCAAACAAACCACCAACTCCTCAGCTCCTTTACACTTATGAAACTGCTGTTTCAGGTTTTGCAGCAAAGCTATCAGCCTCTCAACTCCGATCCTTGAACCGAATAGATGGTTTCCTCTCTGCGACACGTGATGAACTGCTAACTCTCCACACCACACACACTCCTCAGTTTCTTGGCTTACAAAACGGCAAAGGACTTTGGAGTGCTTCTAACTTGGCCTCGGACGTGATCATTGGCTTTGTCGATACAGGGATCTGGCCAGAACATGTTAGCTTCCATGACACAGGCATTTCCCCAGTGCCCTTTCGTTGGAAAGGCACTTGTGAGGAAGGCACAAAGTTCTCAGTTTCAAATTGCAACAAAAAGCTTGTTGGTGCAAGAGCCTTTTTCAAAGGGTATGAGGGCATTGTTGGCAGAATCAACGAAACAGAAGAGTATCGATCCCCTCGCGATTCAAGAGGGCACGGAACTCACACTGCTTCCACTTCAGCTGGAAACCTTGTAAGTCATGCTAGCTTATTTGGTTTGGCCAAGGGCTCAGCCAGCGGTATGAAGCGTACTGCAAGAATCGCAGCCTATAAGGCCTGCTGGCAATTCGGATGTGCCAACTCTGATGTAATGGCTGCAATTGACAGTGCTGTTGCAGATGGGGTAGACATTTTGTCACTCTCTTTGGGAGGCACTTCTAGGCCTTTTTACAGAGACAACATTGCTATTACTACATTTGGAGCAGTTCGAAATGGAGTTTCAGTTTCATGCTCTGCAGGCAATTCAGGCCCTTCTGCTTCAAGTGTTAGCAATGGTGCACCTTGGATTATGACTGTTGCAGCAAGCTACACTGACAGGCGTTTCCCAACAGTTGTGAAGCTCGGGAATGGGCAGATATTCAAAGGGACGTCTCTGTATTCAGGTGAAAAAACTAAGCAATTGCCACTAGTGTATAACAGAACCGCAGGTAATAGCCTAGGAGCTGAGTTTTGCTTTGAGGGTGCACTGGTCAAGAAGCTTGTAAAAGGAAAGATTGTGGTTTGTGAGAGCGGATTGTACACGCGAGCAGGAACTGGAGAGAAGGTGAAGAAGGCAGGGGGAGCCGGAATGCTATTACTCAACAGTGACACCGAAGGAGAGGAACTTGTTGCTGATCCTCACATTTTGCCTGCCGCTTCTCTTGGAGCTTCTGCTGCTAAAGCCATCAGACAATATGTGATGTCATCAAAGAAACCATCGGCTTCGCTTGTGTTCCAAGGCACAGTCTATGGCAACACTGCGCCTGTGATGGCAGCATTTTCATCGAGAGGGCCAAGTTCAGCTGGACCAGACGTGATCAAACCTGATGTGACTGCGCCGGGTGTGAACATTTTGGCAGCCTGGCCACCCAAGCTTAGCCCAAGCATGCTCAAAAGCAATAAGAGAAGCGTGCCGTTTAATATAATCTCAGGCACTTCAATGTCCTGCCCTCATGTTAGTGGCTTAGCCTCTCTTCTCAAGTCGGAACATAATAATTGGTCACCTGCAGAAATCAAATCGGCACTAATGACCACAGCTTATACCATAAACAACAAGCATTCTCCGATTTCTGATGTTGGCTCGAATAGTTCTCAACCAGCTAGCCCTTTTGCATTTGGTTCGGGGCACGTTGATCCAGAAAGTGCTGCTGATCCAGGCCTAGTTTATGACATCACGACTGAAGACTATCTGCTTTACCTCTGCAGCTTAAACTACAACTCTTCCCAAATAGCTCTTTTCTCCAATGGGGTTAATTTCACTTGTCCTGGTACTTCAGTTGTTCAGTCTGGCGACTTGAATTACCCTTCGTTCTCTGTGATTTTCGACCAAGATAGAAGGAAGACGAGTGTGACATACAAGAGGACAGCGAAAAATGTCGGTAGCATTCCTAGTACTTATGCAGTGCAAGTGAAAGAACCAGCTGGAGTTTCAGTGAGTGTTGAGCCTAAAACCTTGAGTTTCAAAAAGATGGGTGAAAAAATGAGCTACAAAGTGAGATTTGTTGCCTTGGGTGGAGCAACTTCAACCAATTCTTCCTTTGGATCTTTAACTTTGGTGGCTGAGAAATATAGAGTTAGAAGTCCCATAGCAGTACTCTGGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0030312 GO:0031012 GO:0043170 GO:0044238 GO:0044421 GO:0044464 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

752

Amino Acids

79.78

Weight (kDa)

9.17

Isoelectric Point (pI)

38.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	13 - 98	6.3e-12	Peptidase inhibitor I9
Peptidase_S8	PF00082	122 - 592	6.4e-48	Subtilase family
PA	PF02225	358 - 446	5.4e-10	PA domain
fn3_6	PF17766	648 - 748	3.2e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0018279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01900
malus_domestica	MD02G1196800.v1.1
prunus_persica	Prupe.2G152000_v2.0.a1
pyrus_communis	pycom02g16010
rosa_chinensis	RchiOBHm_Chr1g0351771
rosa_roxburghii	Rroxscaffold_4G00303560
rosa_rugosa	Rorug01G0221300
rosa_samantha	Rh1AG235300 Rh1DG232800
rosa_wichuraiana	Rw1G020480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1663
AatII	GACGTC	1 cut(s)	1043
Acc36I	ACCTGC	2 cut(s)	1088, 1663
AccBSI	CCGCTC	1 cut(s)	1182
AccI	GTMKAC	1 cut(s)	798
AccII	CGCG	2 cut(s)	594, 1195
AciI	CCGC	6 cut(s)	18, 120, 690, 1095, 1182, 1305
AclWI	GGATC	7 cut(s)	228, 404, 579, 1280, 1778, 1796, 2200
AcoI	YGGCCR	3 cut(s)	401, 672, 1514
AcuI	CTGAAG	3 cut(s)	615, 1851, 1908
AcvI	CACGTG	1 cut(s)	272
AcyI	GRCGYC	1 cut(s)	1040
AfaI	GTAC	6 cut(s)	92, 703, 1190, 1921, 2044, 2244
AfiI	CCNNNNNNNGG	1 cut(s)	453
AflIII	ACRYGT	2 cut(s)	269, 409
AhdI	GACNNNNNGTC	1 cut(s)	805
AhlI	ACTAGT	1 cut(s)	1078
AjiI	CACGTC	2 cut(s)	370, 1465
AjnI	CCWGG	3 cut(s)	1511, 1804, 1915
Alw21I	GWGCWC	2 cut(s)	952, 1138
Alw26I	GTCTC	2 cut(s)	840, 1047
Alw44I	GTGCAC	2 cut(s)	948, 1134
AlwI	GGATC	7 cut(s)	228, 404, 579, 1280, 1778, 1796, 2200
AlwNI	CAGNNNCTG	1 cut(s)	1205
Ama87I	CYCGRG	1 cut(s)	1013
AoxI	GGCC	9 cut(s)	360, 401, 672, 726, 833, 919, 1442, 1514, 1807
ApaLI	GTGCAC	2 cut(s)	948, 1134
ApeKI	GCWGC	9 cut(s)	206, 716, 767, 971, 1325, 1424, 1508, 1796, 1854
AspA2I	CCTAGG	1 cut(s)	1108
AspLEI	GCGC	2 cut(s)	1414, 1489
AspS9I	GGNCC	3 cut(s)	920, 1442, 1457
AsuC2I	CCSGG	1 cut(s)	1491
AsuHPI	GGTGA	6 cut(s)	74, 109, 1067, 1228, 1644, 2135
AsuII	TTCGAA	1 cut(s)	880
AsuNHI	GCTAGC	2 cut(s)	656, 1751
AvaI	CYCGRG	1 cut(s)	1013
AvaII	GGWCC	1 cut(s)	1457
AvrII	CCTAGG	1 cut(s)	1108
BaeGI	GKGCMC	5 cut(s)	447, 609, 952, 1138, 1779
BaeI	ACNNNNGTAYC	2 cut(s)	1911, 1944
BalI	TGGCCA	3 cut(s)	403, 674, 1516
BanII	GRGCYC	1 cut(s)	683
BbrPI	CACGTG	1 cut(s)	272
BbsI	GAAGAC	2 cut(s)	1839, 1997
Bbv12I	GWGCWC	2 cut(s)	952, 1138
BbvCI	CCTCAGC	1 cut(s)	165
BbvI	GCAGC	9 cut(s)	218, 728, 754, 983, 1312, 1436, 1520, 1783, 1866
BccI	CCATC	6 cut(s)	245, 785, 1343, 1375, 1414, 2113
BceAI	ACGGC	2 cut(s)	346, 1549
BcgI	CGANNNNNNTGC	4 cut(s)	1416, 1450, 1752, 1786
BciT130I	CCWGG	3 cut(s)	1513, 1806, 1917
BclI	TGATCA	2 cut(s)	372, 1467
BcnI	CCSGG	1 cut(s)	1491
BcoDI	GTCTC	2 cut(s)	840, 1047
BcuI	ACTAGT	1 cut(s)	1078
BfaI	CTAG	7 cut(s)	657, 831, 1079, 1109, 1752, 1811, 2040
BfmI	CTRYAG	3 cut(s)	906, 1656, 1852
BfuAI	ACCTGC	2 cut(s)	1088, 1663
BglI	GCCNNNNNGGC	1 cut(s)	725
BlnI	CCTAGG	1 cut(s)	1108
BlpI	GCTNAGC	3 cut(s)	682, 1526, 1615
BmcAI	AGTACT	2 cut(s)	2044, 2244
Bme1390I	CCNGG	4 cut(s)	1491, 1513, 1806, 1917
Bme18I	GGWCC	1 cut(s)	1457
BmeRI	GACNNNNNGTC	1 cut(s)	805
BmeT110I	CYCGRG	1 cut(s)	1013
BmgBI	CACGTC	2 cut(s)	370, 1465
BmgT120I	GGNCC	3 cut(s)	920, 1442, 1457
BmiI	GGNNCC	1 cut(s)	1234
BmrFI	CCNGG	4 cut(s)	1491, 1513, 1806, 1917
BmrI	ACTGGG	1 cut(s)	434
BmtI	GCTAGC	2 cut(s)	660, 1755
BmuI	ACTGGG	1 cut(s)	434
BpiI	GAAGAC	2 cut(s)	1839, 1997
BpmI	CTGGAG	2 cut(s)	1227, 2094
Bpu10I	CCTNAGC	1 cut(s)	165
Bpu1102I	GCTNAGC	3 cut(s)	682, 1526, 1615
Bpu14I	TTCGAA	1 cut(s)	880
BpuEI	CTTGAG	3 cut(s)	117, 1613, 2125
BpuMI	CCSGG	1 cut(s)	1491
Bsa29I	ATCGAT	2 cut(s)	108, 583
BsaAI	YACGTR	1 cut(s)	272
BsaHI	GRCGYC	1 cut(s)	1040
BsaJI	CCNNGG	6 cut(s)	363, 675, 953, 1108, 1387, 2157
BsaXI	ACNNNNNCTCC	8 cut(s)	289, 319, 879, 909, 1200, 1230, 2067, 2097
Bsc4I	CCNNNNNNNGG	1 cut(s)	453
Bse1I	ACTGG	3 cut(s)	440, 1143, 1210
Bse3DI	GCAATG	2 cut(s)	852, 949
BseBI	CCWGG	3 cut(s)	1513, 1806, 1917
BseCI	ATCGAT	2 cut(s)	108, 583
BseDI	CCNNGG	6 cut(s)	363, 675, 953, 1108, 1387, 2157
BseGI	GGATG	1 cut(s)	749
BseLI	CCNNNNNNNGG	1 cut(s)	453
BseMI	GCAATG	2 cut(s)	852, 949
BseMII	CTCAG	8 cut(s)	90, 179, 323, 501, 696, 1107, 1593, 2202
BseNI	ACTGG	3 cut(s)	440, 1143, 1210
BseRI	GAGGAG	2 cut(s)	153, 297
BseSI	GKGCMC	5 cut(s)	447, 609, 952, 1138, 1779
BseXI	GCAGC	9 cut(s)	218, 728, 754, 983, 1312, 1436, 1520, 1783, 1866
Bsh1236I	CGCG	2 cut(s)	594, 1195
BshFI	GGCC	9 cut(s)	362, 403, 674, 728, 835, 921, 1444, 1516, 1809
BshVI	ATCGAT	2 cut(s)	108, 583
BsiHKAI	GWGCWC	2 cut(s)	952, 1138
BsiHKCI	CYCGRG	1 cut(s)	1013
BsiSI	CCGG	2 cut(s)	1236, 1490
BslFI	GGGAC	2 cut(s)	1051, 2217
BslI	CCNNNNNNNGG	1 cut(s)	453
BsmAI	GTCTC	2 cut(s)	840, 1047
BsmBI	CGTCTC	1 cut(s)	1047
BsmFI	GGGAC	2 cut(s)	1051, 2217
BsmI	GAATGC	3 cut(s)	1245, 1708, 2034
BsnI	GGCC	9 cut(s)	362, 403, 674, 728, 835, 921, 1444, 1516, 1809
BsoBI	CYCGRG	1 cut(s)	1013
Bsp119I	TTCGAA	1 cut(s)	880
Bsp1286I	GDGCHC	6 cut(s)	447, 609, 683, 952, 1138, 1779
Bsp1407I	TGTACA	1 cut(s)	1188
Bsp1720I	GCTNAGC	3 cut(s)	682, 1526, 1615
Bsp68I	TCGCGA	1 cut(s)	594
BspACI	CCGC	6 cut(s)	18, 120, 690, 1095, 1182, 1305
BspANI	GGCC	9 cut(s)	362, 403, 674, 728, 835, 921, 1444, 1516, 1809
BspCNI	CTCAG	8 cut(s)	89, 178, 322, 500, 695, 1108, 1592, 2203
BspDI	ATCGAT	2 cut(s)	108, 583
BspFNI	CGCG	2 cut(s)	594, 1195
BspLI	GGNNCC	1 cut(s)	1234
BspMAI	CTGCAG	3 cut(s)	910, 1660, 1856
BspMI	ACCTGC	2 cut(s)	1088, 1663
BspOI	GCTAGC	2 cut(s)	660, 1755
BspPI	GGATC	7 cut(s)	228, 404, 579, 1280, 1778, 1796, 2200
BspT104I	TTCGAA	1 cut(s)	880
BsrBI	CCGCTC	1 cut(s)	1182
BsrDI	GCAATG	2 cut(s)	852, 949
BsrGI	TGTACA	1 cut(s)	1188
BsrI	ACTGG	3 cut(s)	440, 1143, 1210
BssECI	CCNNGG	6 cut(s)	363, 675, 953, 1108, 1387, 2157
BssNI	GRCGYC	1 cut(s)	1040
BssT1I	CCWWGG	5 cut(s)	675, 953, 1108, 1387, 2157
Bst2UI	CCWGG	3 cut(s)	1513, 1806, 1917
Bst4CI	ACNGT	6 cut(s)	90, 779, 967, 1003, 1256, 1396
Bst6I	CTCTTC	4 cut(s)	135, 570, 1629, 1876
BstACI	GRCGYC	1 cut(s)	1040
BstAUI	TGTACA	1 cut(s)	1188
BstBAI	YACGTR	1 cut(s)	272
BstBI	TTCGAA	1 cut(s)	880
BstEII	GGTNACC	1 cut(s)	1650
BstF5I	GGATG	1 cut(s)	749
BstFNI	CGCG	2 cut(s)	594, 1195
BstHHI	GCGC	2 cut(s)	1414, 1489
BstMAI	GTCTC	2 cut(s)	840, 1047
BstNI	CCWGG	3 cut(s)	1513, 1806, 1917
BstNSI	RCATGY	2 cut(s)	413, 1540
BstPI	GGTNACC	1 cut(s)	1650
BstSCI	CCNGG	4 cut(s)	1489, 1511, 1804, 1915
BstSFI	CTRYAG	3 cut(s)	906, 1656, 1852
BstSLI	GKGCMC	5 cut(s)	447, 609, 952, 1138, 1779
BstUI	CGCG	2 cut(s)	594, 1195
BstV1I	GCAGC	9 cut(s)	218, 728, 754, 983, 1312, 1436, 1520, 1783, 1866
BstV2I	GAAGAC	2 cut(s)	1839, 1997
BstX2I	RGATCY	2 cut(s)	396, 2192
BstYI	RGATCY	2 cut(s)	396, 2192
Bsu15I	ATCGAT	2 cut(s)	108, 583
BsuRI	GGCC	9 cut(s)	362, 403, 674, 728, 835, 921, 1444, 1516, 1809
BsuTUI	ATCGAT	2 cut(s)	108, 583
BtrI	CACGTC	2 cut(s)	370, 1465
BtsCI	GGATG	1 cut(s)	749
BtsI	GCAGTG	3 cut(s)	618, 1407, 2058
BtsIMutI	CAGTG	9 cut(s)	447, 618, 784, 981, 1136, 1261, 1407, 2058, 2088
BtuMI	TCGCGA	1 cut(s)	594
BveI	ACCTGC	2 cut(s)	1088, 1663
CaiI	CAGNNNCTG	1 cut(s)	1205
CfoI	GCGC	2 cut(s)	1414, 1489
Cfr13I	GGNCC	3 cut(s)	920, 1442, 1457
ClaI	ATCGAT	2 cut(s)	108, 583
Csp6I	GTAC	6 cut(s)	91, 702, 1189, 1920, 2043, 2243
CviAII	CATG	7 cut(s)	52, 410, 422, 653, 900, 1537, 1604
CviQI	GTAC	6 cut(s)	91, 702, 1189, 1920, 2043, 2243
DriI	GACNNNNNGTC	1 cut(s)	805
EaeI	YGGCCR	3 cut(s)	401, 672, 1514
Eam1104I	CTCTTC	4 cut(s)	135, 570, 1629, 1876
Eam1105I	GACNNNNNGTC	1 cut(s)	805
EarI	CTCTTC	4 cut(s)	135, 570, 1629, 1876
Eco130I	CCWWGG	5 cut(s)	675, 953, 1108, 1387, 2157
Eco147I	AGGCCT	3 cut(s)	728, 835, 1809
Eco24I	GRGCYC	1 cut(s)	683
Eco47I	GGWCC	1 cut(s)	1457
Eco57I	CTGAAG	3 cut(s)	615, 1851, 1908
Eco72I	CACGTG	1 cut(s)	272
Eco88I	CYCGRG	1 cut(s)	1013
Eco91I	GGTNACC	1 cut(s)	1650
EcoO109I	RGGNCCY	1 cut(s)	920
EcoO65I	GGTNACC	1 cut(s)	1650
EcoRII	CCWGG	3 cut(s)	1511, 1804, 1915
EcoT14I	CCWWGG	5 cut(s)	675, 953, 1108, 1387, 2157
EcoT38I	GRGCYC	1 cut(s)	683
ErhI	CCWWGG	5 cut(s)	675, 953, 1108, 1387, 2157
Esp3I	CGTCTC	1 cut(s)	1047
FaeI	CATG	7 cut(s)	55, 413, 425, 656, 903, 1540, 1607
FaqI	GGGAC	2 cut(s)	1051, 2217
FatI	CATG	7 cut(s)	51, 409, 421, 652, 899, 1536, 1603
FbaI	TGATCA	2 cut(s)	372, 1467
FblI	GTMKAC	1 cut(s)	798
FokI	GGATG	1 cut(s)	756
FriOI	GRGCYC	1 cut(s)	683
FspBI	CTAG	7 cut(s)	657, 831, 1079, 1109, 1752, 1811, 2040
GlaI	GCGC	2 cut(s)	1413, 1488
GsuI	CTGGAG	2 cut(s)	1227, 2094
HaeIII	GGCC	9 cut(s)	362, 403, 674, 728, 835, 921, 1444, 1516, 1809
HapII	CCGG	2 cut(s)	1236, 1490
HhaI	GCGC	2 cut(s)	1414, 1489
Hin1I	GRCGYC	1 cut(s)	1040
Hin1II	CATG	7 cut(s)	55, 413, 425, 656, 903, 1540, 1607
Hin6I	GCGC	2 cut(s)	1412, 1487
HinP1I	GCGC	2 cut(s)	1412, 1487
HindIII	AAGCTT	3 cut(s)	509, 1148, 1523
HinfI	GANTC	5 cut(s)	74, 110, 561, 596, 711
HpaII	CCGG	2 cut(s)	1236, 1490
HphI	GGTGA	6 cut(s)	74, 109, 1067, 1228, 1644, 2135
Hpy166II	GTNNAC	6 cut(s)	49, 799, 950, 1136, 1191, 1498
Hpy188I	TCNGA	9 cut(s)	79, 233, 367, 743, 757, 1340, 1636, 1716, 1723
Hpy188III	TCNNGA	7 cut(s)	593, 600, 1015, 1144, 1437, 1787, 1825
Hpy8I	GTNNAC	6 cut(s)	49, 799, 950, 1136, 1191, 1498
HpyAV	CCTTC	7 cut(s)	467, 933, 1207, 1216, 1259, 1965, 1980
HpyCH4III	ACNGT	6 cut(s)	90, 779, 967, 1003, 1256, 1396
HpyCH4IV	ACGT	5 cut(s)	271, 369, 1040, 1464, 1779
HpySE526I	ACGT	5 cut(s)	271, 369, 1040, 1464, 1779
Hsp92I	GRCGYC	1 cut(s)	1040
Hsp92II	CATG	7 cut(s)	55, 413, 425, 656, 903, 1540, 1607
HspAI	GCGC	2 cut(s)	1412, 1487
Ksp22I	TGATCA	2 cut(s)	372, 1467
LmnI	GCTCC	6 cut(s)	174, 872, 1112, 1232, 1316, 2165
Lsp1109I	GCAGC	9 cut(s)	218, 728, 754, 983, 1312, 1436, 1520, 1783, 1866
MaeI	CTAG	7 cut(s)	657, 831, 1079, 1109, 1752, 1811, 2040
MaeII	ACGT	5 cut(s)	271, 369, 1040, 1464, 1779
MaeIII	GTNAC	5 cut(s)	807, 1256, 1480, 1650, 1999
MbiI	CCGCTC	1 cut(s)	1182
MboII	GAAGA	9 cut(s)	41, 152, 587, 1231, 1616, 1844, 1863, 2002, 2175
MfeI	CAATTG	2 cut(s)	771, 1070
MflI	RGATCY	2 cut(s)	396, 2192
MhlI	GDGCHC	6 cut(s)	447, 609, 683, 952, 1138, 1779
MlsI	TGGCCA	3 cut(s)	403, 674, 1516
MluCI	AATT	9 cut(s)	496, 737, 771, 913, 1070, 1645, 1903, 1948, 2179
MluNI	TGGCCA	3 cut(s)	403, 674, 1516
MlyI	GAGTC	1 cut(s)	68
MmeI	TCCRAC	2 cut(s)	434, 1614
Mox20I	TGGCCA	3 cut(s)	403, 674, 1516
MscI	TGGCCA	3 cut(s)	403, 674, 1516
MseI	TTAA	5 cut(s)	23, 1569, 1859, 1902, 2198
MslI	CAYNNNNRTG	2 cut(s)	1589, 1608
Msp20I	TGGCCA	3 cut(s)	403, 674, 1516
MspA1I	CMGCKG	4 cut(s)	635, 690, 1454, 2072
MspI	CCGG	2 cut(s)	1236, 1490
MspR9I	CCNGG	4 cut(s)	1491, 1513, 1806, 1917
MunI	CAATTG	2 cut(s)	771, 1070
Mva1269I	GAATGC	3 cut(s)	1245, 1708, 2034
MvaI	CCWGG	3 cut(s)	1513, 1806, 1917
MvnI	CGCG	2 cut(s)	594, 1195
NciI	CCSGG	1 cut(s)	1491
NheI	GCTAGC	2 cut(s)	656, 1751
NlaIII	CATG	7 cut(s)	55, 413, 425, 656, 903, 1540, 1607
NlaIV	GGNNCC	1 cut(s)	1234
NmuCI	GTSAC	5 cut(s)	807, 1256, 1480, 1650, 1999
NruI	TCGCGA	1 cut(s)	594
NspI	RCATGY	2 cut(s)	413, 1540
NspV	TTCGAA	1 cut(s)	880
PaeI	GCATGC	1 cut(s)	1540
PaqCI	CACCTGC	1 cut(s)	1663
PceI	AGGCCT	3 cut(s)	728, 835, 1809
PciI	ACATGT	1 cut(s)	409
PctI	GAATGC	3 cut(s)	1245, 1708, 2034
PfeI	GAWTC	4 cut(s)	110, 561, 596, 711
PleI	GAGTC	1 cut(s)	68
PmaCI	CACGTG	1 cut(s)	272
PmlI	CACGTG	1 cut(s)	272
PpsI	GAGTC	1 cut(s)	68
Ppu21I	YACGTR	1 cut(s)	272
PscI	ACATGT	1 cut(s)	409
Psp6I	CCWGG	3 cut(s)	1511, 1804, 1915
PspCI	CACGTG	1 cut(s)	272
PspEI	GGTNACC	1 cut(s)	1650
PspGI	CCWGG	3 cut(s)	1511, 1804, 1915
PspN4I	GGNNCC	1 cut(s)	1234
PspPI	GGNCC	3 cut(s)	920, 1442, 1457
PstI	CTGCAG	3 cut(s)	910, 1660, 1856
PstNI	CAGNNNCTG	1 cut(s)	1205
PsuI	RGATCY	2 cut(s)	396, 2192
PvuII	CAGCTG	3 cut(s)	635, 1454, 2072
RruI	TCGCGA	1 cut(s)	594
RsaI	GTAC	6 cut(s)	92, 703, 1190, 1921, 2044, 2244
RsaNI	GTAC	6 cut(s)	91, 702, 1189, 1920, 2043, 2243
RseI	CAYNNNNRTG	2 cut(s)	1589, 1608
SaqAI	TTAA	5 cut(s)	23, 1569, 1859, 1902, 2198
Sau96I	GGNCC	3 cut(s)	920, 1442, 1457
ScaI	AGTACT	2 cut(s)	2044, 2244
SchI	GAGTC	1 cut(s)	68
ScrFI	CCNGG	4 cut(s)	1491, 1513, 1806, 1917
SduI	GDGCHC	6 cut(s)	447, 609, 683, 952, 1138, 1779
SfcI	CTRYAG	3 cut(s)	906, 1656, 1852
SfuI	TTCGAA	1 cut(s)	880
SinI	GGWCC	1 cut(s)	1457
SmiMI	CAYNNNNRTG	2 cut(s)	1589, 1608
SmlI	CTYRAG	3 cut(s)	132, 1628, 2104
SmoI	CTYRAG	3 cut(s)	132, 1628, 2104
SpeI	ACTAGT	1 cut(s)	1078
SphI	GCATGC	1 cut(s)	1540
Sse9I	AATT	9 cut(s)	496, 737, 771, 913, 1070, 1645, 1903, 1948, 2179
SseBI	AGGCCT	3 cut(s)	728, 835, 1809
SsiI	CCGC	6 cut(s)	18, 120, 690, 1095, 1182, 1305
SspMI	CTAG	7 cut(s)	657, 831, 1079, 1109, 1752, 1811, 2040
StuI	AGGCCT	3 cut(s)	728, 835, 1809
StyD4I	CCNGG	4 cut(s)	1489, 1511, 1804, 1915
StyI	CCWWGG	5 cut(s)	675, 953, 1108, 1387, 2157
TaaI	ACNGT	6 cut(s)	90, 779, 967, 1003, 1256, 1396
TaiI	ACGT	5 cut(s)	274, 372, 1043, 1467, 1782
TaqI	TCGA	7 cut(s)	108, 387, 583, 880, 1436, 1733, 1974
TasI	AATT	9 cut(s)	496, 737, 771, 913, 1070, 1645, 1903, 1948, 2179
TatI	WGTACW	3 cut(s)	1188, 2042, 2242
TauI	GCSGC	1 cut(s)	1307
TfiI	GAWTC	4 cut(s)	110, 561, 596, 711
Tru1I	TTAA	5 cut(s)	23, 1569, 1859, 1902, 2198
Tru9I	TTAA	5 cut(s)	23, 1569, 1859, 1902, 2198
TscAI	CASTG	9 cut(s)	447, 625, 784, 988, 1143, 1261, 1414, 2058, 2088
TseFI	GTSAC	5 cut(s)	807, 1256, 1480, 1650, 1999
TseI	GCWGC	9 cut(s)	206, 716, 767, 971, 1325, 1424, 1508, 1796, 1854
Tsp45I	GTSAC	5 cut(s)	807, 1256, 1480, 1650, 1999
TspDTI	ATGAA	5 cut(s)	198, 291, 710, 888, 1422
TspGWI	ACGGA	2 cut(s)	86, 624
TspRI	CASTG	9 cut(s)	447, 625, 784, 988, 1143, 1261, 1414, 2058, 2088
VneI	GTGCAC	2 cut(s)	948, 1134
VpaK11BI	GGWCC	1 cut(s)	1457
XceI	RCATGY	2 cut(s)	413, 1540
XmaJI	CCTAGG	1 cut(s)	1108
XmiI	GTMKAC	1 cut(s)	798
XspI	CTAG	7 cut(s)	657, 831, 1079, 1109, 1752, 1811, 2040
ZraI	GACGTC	1 cut(s)	1041
ZrmI	AGTACT	2 cut(s)	2044, 2244

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.