RchiOBHm_Chr1g0351771

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018279
Physical Location & Seq
Forward (+)
45006970 .. 45009724
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57752

Sequence Viewer

Length: 2310 bp
ATGATGGTTAGAGCATTCTTGTTGTTCTTGGCTTTCATGGCTACAACTAGAACAGCTCTGATGGAAACGCAGACGTACATTGTTCACATGGACAAGGCGAAAATCCCTGCCTCGGGTCACAAAGATCAACACTACTACTACAAAGCAGTGATGGATTCCATTACCGAACTCTCATCTCAAGAAGAAGAGGAAGAAGAAGGTGGCAAAGCATCAGCTCCTCAGCTTCTTTACATCTACAACACTGCTATTTCGGGTTTTGCAGCAAAGCTCTCAAGCAATCAACTCAAATCCTTGAGTCAAATCCATGGTTTCTTAGATGCCACACCTGATGAGCTTCTAACTCTCCACACCACACACACTCCTCAGTTTCTTGGCTTACAAGAAGGGAAGGGACTTTGGAGTGCTCCCAACTTGGCCACGGATGTGATCATTGGGGTTGTTGACACGGGAGTCTGGCCAGAACATATTAGCTTCAAGGACAAAGGAATGTCCTCTGTACCCTCTCACTGGAATGGCACTTGTGAACCAGGCACAAGGTTCTCACACTCAAATTGTAACAAAAAGCTTATTGGTGCAAGAGCCTTTTTCCAAGGGTATGAGGCCATTGTCGGAAGAGTCAATGAAACAGTGGATTATAGATCCCCTCGTGACTCCGAAGGACATGGAACTCACACTGCTTCAACTGCAGGCGGTAATGTTGTCAAACAAGCAAGCTTATTTGGCTTAGCAAAGGGATCGGCCAGTGGAATGAGGCATTCTTCAAGAATTGCAGCCTATAAGGCCTGTTGGCCATTGGGGTGTGCTAACTCTGATGTAATGGCTGCAATTGACAGTGCTATTTCTGATGGGGTAGACATTTTGTCACTCTCATTGGGAGGTCTTCCGAGACCTTATTTTCGTGACAATATTGCTATAGCTTCATTTGGTGCAGTCCAACACGGAGTTTCCGTGTCATCTTCAGCTGGAAATTCAGGTCCCTTTCGCTCCAGTGTTAGCAATACCGCACCTTGGATCATGACTGTTGCTGCTAGCTACACTGACCGAAACTTCCCAGTGATAGTGAAGCTTGGGAATGGACAAACTTATGAAGGGTCATCACTATATTCAGGTAAGAAAACTAAGCAGTTGCCACTAGTGTATAACAGAACAGCAGGTGGCCAAGGAGCTGAGCATTGTGTTGATGGCTCGCTTGACACAAAGCTTGTGAAGGGAAAGATTGTAGCATGTGAGCGAGGAACACACAGCCGGACTGAAATTGGAGAGGAAGTGAAGAAAGCAGGAGGAGCCGGAATGCTTCTACTCAATAATGAAGCTCAAGGAGAGGAACTACTTGCTGATGCTCACATTTTGCCAGCCACTTCTCTTGGAGCTTCGGCTTCTAAAGCCGTGAGAAAATACGCAGGTGCAGCAAAGAAACCTACAGCTTTGCTTCTGTTCCAAGGCACGGTCTTTGGTAAAACTGCTCCGGTGATGGCAGCATTTTCATCTAGGGGGCCAAGCTCAGTTGGACCGGACTTGATCAAGCCAGATGTGACTGCTCCTGGTGTCAACATTTTGGCTGCTTGGCCAGCCAAGACTAGCCCAACCAGGCTTAAAAGTGACAAGAGAAAGGTGTTGTTTAACATGGTCTCAGGCACTTCAATGTCTTGCCCTCATGTTAGTGGCTTAGCTGCTCTTATCAAATCAGTGCACAAGGATTGGTCACCTGCAGCAATCAAATCTGCACTTATGACTACAGCTTATACACTAAATAACAAAGGCTCTCCGATTTCTGATTTTGGCTCAAAAAATCAATCAGCTAACCCTTTTGCATTTGGTTCGGGGCATGTCGACCCAGAAAGTGCTGCCGATCCAGGACTAATTTATGATATCACCAGCAAAGATTACTTGCTCTATTTATGTAGCTTGAATTACACATCTTCCCAAATAGCTCTTTTCTCCGGTGGGATTAGTAACTTCACTTGTCCTAGCAACAGTGCAGTTCTTCTTCAGCCCGGCGACCTGAACTACCCTTCATTCTCAGTGGTTTTCAAAAAAGGCGGAATGAAAATGAGTGTGACATACAAGAGGACAGTGACAAATGTTGGTGCCATTAACCCTAGTACTTATGCAGTGCAAGTGGAAGCACCCATTGGAGCATCAGTTACTGTTGAACCTAGGAAGTTGGTTTTTAAGAAGATGGGTGAGAAATTGAGCTACAAGGTGAGTTTTGTTGGAGTGAGTACTAAAATCAATTCCTCATTTGGATCCTTAGTTTGGGTGAGTGAGAAATATAGGGTTAGAAGTCCCATTGCAGTGATTTGGGTGTAA

Protein Analysis

769

Amino Acids

81.8

Weight (kDa)

9.2

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 25 - 116 2e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 594 7.4e-49 Subtilase family
PA PF02225 375 - 461 1.2e-10 PA domain
fn3_6 PF17766 667 - 766 3.7e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01900
malus_domestica MD02G1196800.v1.1
prunus_persica Prupe.2G152000_v2.0.a1
pyrus_communis pycom02g16010
rosa_chinensis RchiOBHm_Chr1g0351771
rosa_roxburghii Rroxscaffold_4G00303560
rosa_rugosa Rorug01G0221300
rosa_samantha Rh1AG235300 Rh1DG232800
rosa_wichuraiana Rw1G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1142, 1391, 1714
AasI GACNNNNNNGTC 1 cut(s) 449
Acc36I ACCTGC 3 cut(s) 1142, 1391, 1714
AccB1I GGYRCC 1 cut(s) 2087
AccI GTMKAC 2 cut(s) 852, 1830
AciI CCGC 3 cut(s) 690, 1002, 2040
AclWI GGATC 6 cut(s) 633, 742, 1019, 1844, 2241, 2254
AcoI YGGCCR 6 cut(s) 414, 455, 738, 788, 1156, 1565
AcsI RAATTY 1 cut(s) 967
AcuI CTGAAG 2 cut(s) 942, 1973
AfaI GTAC 4 cut(s) 77, 498, 2104, 2224
AfiI CCNNNNNNNGG 6 cut(s) 112, 113, 507, 1008, 1444, 2256
AgsI TTSAA 7 cut(s) 475, 681, 762, 1641, 1909, 2032, 2153
AhdI GACNNNNNGTC 1 cut(s) 859
AhlI ACTAGT 1 cut(s) 1132
AjnI CCWGG 4 cut(s) 526, 1540, 1586, 1852
AjuI GAANNNNNNNTTGG 2 cut(s) 2115, 2147
AleI CACNNNNGTG 1 cut(s) 422
Alw21I GWGCWC 2 cut(s) 406, 1692
Alw26I GTCTC 2 cut(s) 880, 1633
Alw44I GTGCAC 1 cut(s) 1688
AlwI GGATC 6 cut(s) 633, 742, 1019, 1844, 2241, 2254
AlwNI CAGNNNCTG 1 cut(s) 2147
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 9 cut(s) 414, 455, 600, 738, 780, 788, 1156, 1493, 1565
ApaLI GTGCAC 1 cut(s) 1688
ApoI RAATTY 1 cut(s) 967
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
AspA2I CCTAGG 1 cut(s) 2156
AspS9I GGNCC 3 cut(s) 974, 1493, 1508
AsuC2I CCSGG 1 cut(s) 1995
AsuHPI GGTGA 6 cut(s) 1480, 1695, 1864, 2195, 2215, 2272
AsuNHI GCTAGC 1 cut(s) 1028
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 2 cut(s) 974, 1508
AvrII CCTAGG 1 cut(s) 2156
BaeGI GKGCMC 1 cut(s) 1692
BalI TGGCCA 5 cut(s) 416, 457, 790, 1158, 1567
BamHI GGATCC 1 cut(s) 2246
BanI GGYRCC 1 cut(s) 2087
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 1 cut(s) 872
Bbv12I GWGCWC 2 cut(s) 406, 1692
BbvCI CCTCAGC 1 cut(s) 219
BccI CCATC 6 cut(s) 55, 145, 839, 1175, 1465, 2173
BceAI ACGGC 1 cut(s) 1370
BcgI CGANNNNNNTGC 4 cut(s) 717, 751, 1800, 1834
BciT130I CCWGG 4 cut(s) 528, 1542, 1588, 1854
BclI TGATCA 2 cut(s) 426, 1518
BcnI CCSGG 1 cut(s) 1995
BcoDI GTCTC 2 cut(s) 880, 1633
BcuI ACTAGT 1 cut(s) 1132
BfaI CTAG 8 cut(s) 48, 1029, 1133, 1488, 1578, 1968, 2100, 2157
BfmI CTRYAG 5 cut(s) 684, 912, 1419, 1707, 1734
BfuAI ACCTGC 3 cut(s) 1142, 1391, 1714
BglI GCCNNNNNGGC 1 cut(s) 779
BlnI CCTAGG 1 cut(s) 2156
BlpI GCTNAGC 3 cut(s) 724, 1167, 1666
BmcAI AGTACT 2 cut(s) 2104, 2224
Bme1390I CCNGG 5 cut(s) 528, 1542, 1588, 1854, 1995
Bme18I GGWCC 2 cut(s) 974, 1508
BmeRI GACNNNNNGTC 1 cut(s) 859
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 3 cut(s) 974, 1493, 1508
BmiI GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
BmrFI CCNGG 5 cut(s) 528, 1542, 1588, 1854, 1995
BmrI ACTGGG 1 cut(s) 1046
BmsI GCATC 4 cut(s) 218, 307, 1327, 2147
BmtI GCTAGC 1 cut(s) 1032
BmuI ACTGGG 1 cut(s) 1046
BpiI GAAGAC 1 cut(s) 872
BpmI CTGGAG 1 cut(s) 970
Bpu10I CCTNAGC 1 cut(s) 219
Bpu1102I GCTNAGC 3 cut(s) 724, 1167, 1666
BpuEI CTTGAG 4 cut(s) 162, 256, 313, 1299
BpuMI CCSGG 1 cut(s) 1995
BsaI GGTCTC 2 cut(s) 880, 1633
BsaJI CCNNGG 8 cut(s) 111, 304, 417, 589, 1007, 1159, 1438, 2156
BsaWI WCCGGW 3 cut(s) 1465, 1510, 1940
BsaXI ACNNNNNCTCC 8 cut(s) 343, 373, 933, 963, 1251, 1281, 2127, 2157
Bsc4I CCNNNNNNNGG 6 cut(s) 112, 113, 507, 1008, 1444, 2256
Bse1I ACTGG 4 cut(s) 512, 741, 987, 1052
Bse3DI GCAATG 1 cut(s) 2289
BseBI CCWGG 4 cut(s) 528, 1542, 1588, 1854
BseDI CCNNGG 8 cut(s) 111, 304, 417, 589, 1007, 1159, 1438, 2156
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 6 cut(s) 112, 113, 507, 1008, 1444, 2256
BseMI GCAATG 1 cut(s) 2289
BseMII CTCAG 6 cut(s) 233, 377, 1158, 1515, 1644, 2034
BseNI ACTGG 4 cut(s) 512, 741, 987, 1052
BseRI GAGGAG 3 cut(s) 207, 351, 1296
BseSI GKGCMC 1 cut(s) 1692
BsgI GTGCAG 4 cut(s) 948, 1425, 1707, 1998
BshFI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
BshNI GGYRCC 1 cut(s) 2087
BsiHKAI GWGCWC 2 cut(s) 406, 1692
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 6 cut(s) 1246, 1287, 1466, 1511, 1941, 1995
BslFI GGGAC 3 cut(s) 405, 960, 2271
BslI CCNNNNNNNGG 6 cut(s) 112, 113, 507, 1008, 1444, 2256
BsmAI GTCTC 2 cut(s) 880, 1633
BsmFI GGGAC 3 cut(s) 405, 960, 2271
BsmI GAATGC 3 cut(s) 14, 754, 1296
BsnI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
Bso31I GGTCTC 2 cut(s) 880, 1633
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 2 cut(s) 406, 1692
Bsp143I GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
Bsp1720I GCTNAGC 3 cut(s) 724, 1167, 1666
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 3 cut(s) 690, 1002, 2040
BspANI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
BspCNI CTCAG 6 cut(s) 232, 376, 1159, 1514, 1643, 2033
BspHI TCATGA 1 cut(s) 1014
BspLI GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
BspMAI CTGCAG 2 cut(s) 688, 1711
BspMI ACCTGC 3 cut(s) 1142, 1391, 1714
BspOI GCTAGC 1 cut(s) 1032
BspPI GGATC 6 cut(s) 633, 742, 1019, 1844, 2241, 2254
BspT107I GGYRCC 1 cut(s) 2087
BspTNI GGTCTC 2 cut(s) 880, 1633
BsrDI GCAATG 1 cut(s) 2289
BsrI ACTGG 4 cut(s) 512, 741, 987, 1052
BssECI CCNNGG 8 cut(s) 111, 304, 417, 589, 1007, 1159, 1438, 2156
BssMI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 6 cut(s) 304, 589, 1007, 1159, 1438, 2156
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 4 cut(s) 528, 1542, 1588, 1854
Bst4CI ACNGT 7 cut(s) 628, 833, 1021, 1447, 1976, 2074, 2149
Bst6I CTCTTC 2 cut(s) 180, 607
BstC8I GCNNGC 6 cut(s) 688, 712, 1030, 1187, 1353, 1569
BstDSI CCRYGG 2 cut(s) 304, 417
BstEII GGTNACC 1 cut(s) 1701
BstF5I GGATG 1 cut(s) 427
BstKTI GATC 8 cut(s) 127, 429, 641, 737, 1014, 1521, 1852, 2249
BstMAI GTCTC 2 cut(s) 880, 1633
BstMBI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
BstMWI GCNNNNNNNGC 8 cut(s) 38, 683, 720, 779, 1283, 1382, 1406, 1568
BstNI CCWGG 4 cut(s) 528, 1542, 1588, 1854
BstNSI RCATGY 2 cut(s) 1227, 1829
BstPI GGTNACC 1 cut(s) 1701
BstSCI CCNGG 5 cut(s) 526, 1540, 1586, 1852, 1993
BstSFI CTRYAG 5 cut(s) 684, 912, 1419, 1707, 1734
BstSLI GKGCMC 1 cut(s) 1692
BstV2I GAAGAC 1 cut(s) 872
BstX2I RGATCY 2 cut(s) 638, 2246
BstYI RGATCY 2 cut(s) 638, 2246
BsuRI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
BtgI CCRYGG 2 cut(s) 304, 417
BtsCI GGATG 1 cut(s) 427
BtsI GCAGTG 5 cut(s) 153, 240, 672, 2118, 2301
BveI ACCTGC 3 cut(s) 1142, 1391, 1714
Cac8I GCNNGC 6 cut(s) 688, 712, 1030, 1187, 1353, 1569
CaiI CAGNNNCTG 1 cut(s) 2147
CciI TCATGA 1 cut(s) 1014
Cfr13I GGNCC 3 cut(s) 974, 1493, 1508
Csp6I GTAC 4 cut(s) 76, 497, 2103, 2223
CspCI CAANNNNNGTGG 2 cut(s) 1345, 1380
CviAII CATG 9 cut(s) 37, 88, 305, 662, 1015, 1224, 1624, 1655, 1826
CviQI GTAC 4 cut(s) 76, 497, 2103, 2223
DpnI GATC 8 cut(s) 126, 428, 640, 736, 1013, 1520, 1851, 2248
DpnII GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
DrdI GACNNNNNNGTC 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 859
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 6 cut(s) 414, 455, 738, 788, 1156, 1565
Eam1104I CTCTTC 2 cut(s) 180, 607
Eam1105I GACNNNNNGTC 1 cut(s) 859
EarI CTCTTC 2 cut(s) 180, 607
EciI GGCGGA 1 cut(s) 2055
Eco130I CCWWGG 6 cut(s) 304, 589, 1007, 1159, 1438, 2156
Eco147I AGGCCT 1 cut(s) 782
Eco31I GGTCTC 2 cut(s) 880, 1633
Eco32I GATATC 1 cut(s) 1870
Eco47I GGWCC 2 cut(s) 974, 1508
Eco57I CTGAAG 2 cut(s) 942, 1973
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 1701
EcoO109I RGGNCCY 1 cut(s) 974
EcoO65I GGTNACC 1 cut(s) 1701
EcoRII CCWGG 4 cut(s) 526, 1540, 1586, 1852
EcoRV GATATC 1 cut(s) 1870
EcoT14I CCWWGG 6 cut(s) 304, 589, 1007, 1159, 1438, 2156
ErhI CCWWGG 6 cut(s) 304, 589, 1007, 1159, 1438, 2156
FaeI CATG 9 cut(s) 40, 91, 308, 665, 1018, 1227, 1627, 1658, 1829
FaqI GGGAC 3 cut(s) 405, 960, 2271
FatI CATG 9 cut(s) 36, 87, 304, 661, 1014, 1223, 1623, 1654, 1825
FbaI TGATCA 2 cut(s) 426, 1518
FblI GTMKAC 2 cut(s) 852, 1830
FokI GGATG 1 cut(s) 434
FspBI CTAG 8 cut(s) 48, 1029, 1133, 1488, 1578, 1968, 2100, 2157
GsuI CTGGAG 1 cut(s) 970
HaeIII GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
HapII CCGG 6 cut(s) 1246, 1287, 1466, 1511, 1941, 1995
Hin1II CATG 9 cut(s) 40, 91, 308, 665, 1018, 1227, 1627, 1658, 1829
HincII GTYRAC 3 cut(s) 442, 1549, 1831
HindII GTYRAC 3 cut(s) 442, 1549, 1831
HindIII AAGCTT 4 cut(s) 563, 712, 1064, 1199
HinfI GANTC 5 cut(s) 155, 295, 450, 615, 650
HpaII CCGG 6 cut(s) 1246, 1287, 1466, 1511, 1941, 1995
HphI GGTGA 6 cut(s) 1480, 1695, 1864, 2195, 2215, 2272
Hpy166II GTNNAC 7 cut(s) 85, 442, 524, 853, 1549, 1690, 1831
Hpy188I TCNGA 8 cut(s) 60, 611, 655, 811, 844, 885, 1767, 1774
Hpy188III TCNNGA 5 cut(s) 179, 647, 762, 899, 1015
Hpy8I GTNNAC 7 cut(s) 85, 442, 524, 853, 1549, 1690, 1831
HpyAV CCTTC 7 cut(s) 191, 377, 382, 650, 1082, 1201, 2022
HpyCH4III ACNGT 7 cut(s) 628, 833, 1021, 1447, 1976, 2074, 2149
HpyCH4IV ACGT 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 683, 720, 779, 1283, 1382, 1406, 1568
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 9 cut(s) 40, 91, 308, 665, 1018, 1227, 1627, 1658, 1829
Ksp22I TGATCA 2 cut(s) 426, 1518
Kzo9I GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
LmnI GCTCC 9 cut(s) 220, 409, 989, 1163, 1283, 1367, 1468, 1543, 2135
LweI GCATC 4 cut(s) 218, 307, 1327, 2147
MaeI CTAG 8 cut(s) 48, 1029, 1133, 1488, 1578, 1968, 2100, 2157
MaeII ACGT 1 cut(s) 74
MalI GATC 8 cut(s) 126, 428, 640, 736, 1013, 1520, 1851, 2248
MboI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 2 cut(s) 638, 2246
MhlI GDGCHC 2 cut(s) 406, 1692
MlsI TGGCCA 5 cut(s) 416, 457, 790, 1158, 1567
MluCI AATT 9 cut(s) 550, 765, 825, 967, 1254, 1860, 1909, 2189, 2233
MluNI TGGCCA 5 cut(s) 416, 457, 790, 1158, 1567
MlyI GAGTC 4 cut(s) 304, 459, 624, 644
MmeI TCCRAC 4 cut(s) 589, 958, 1486, 2194
Mox20I TGGCCA 5 cut(s) 416, 457, 790, 1158, 1567
MscI TGGCCA 5 cut(s) 416, 457, 790, 1158, 1567
MseI TTAA 4 cut(s) 1593, 1620, 2094, 2172
MslI CAYNNNNRTG 6 cut(s) 422, 510, 796, 1640, 1659, 2294
Msp20I TGGCCA 5 cut(s) 416, 457, 790, 1158, 1567
MspA1I CMGCKG 1 cut(s) 962
MspI CCGG 6 cut(s) 1246, 1287, 1466, 1511, 1941, 1995
MspR9I CCNGG 5 cut(s) 528, 1542, 1588, 1854, 1995
MunI CAATTG 1 cut(s) 825
Mva1269I GAATGC 3 cut(s) 14, 754, 1296
MvaI CCWGG 4 cut(s) 528, 1542, 1588, 1854
MwoI GCNNNNNNNGC 8 cut(s) 38, 683, 720, 779, 1283, 1382, 1406, 1568
NciI CCSGG 1 cut(s) 1995
NcoI CCATGG 1 cut(s) 304
NdeII GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
NheI GCTAGC 1 cut(s) 1028
NlaIII CATG 9 cut(s) 40, 91, 308, 665, 1018, 1227, 1627, 1658, 1829
NlaIV GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
NmuCI GTSAC 9 cut(s) 116, 647, 861, 899, 1531, 1598, 1701, 2056, 2074
NspI RCATGY 2 cut(s) 1227, 1829
OliI CACNNNNGTG 1 cut(s) 422
PagI TCATGA 1 cut(s) 1014
PaqCI CACCTGC 3 cut(s) 1142, 1391, 1714
PceI AGGCCT 1 cut(s) 782
PcsI WCGNNNNNNNCGW 1 cut(s) 945
PctI GAATGC 3 cut(s) 14, 754, 1296
PfeI GAWTC 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 1852
PleI GAGTC 4 cut(s) 303, 458, 623, 644
PpsI GAGTC 4 cut(s) 303, 458, 623, 644
PpuMI RGGWCCY 1 cut(s) 974
Psp5II RGGWCCY 1 cut(s) 974
Psp6I CCWGG 4 cut(s) 526, 1540, 1586, 1852
PspEI GGTNACC 1 cut(s) 1701
PspGI CCWGG 4 cut(s) 526, 1540, 1586, 1852
PspN4I GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
PspPI GGNCC 3 cut(s) 974, 1493, 1508
PspPPI RGGWCCY 1 cut(s) 974
PstI CTGCAG 2 cut(s) 688, 1711
PstNI CAGNNNCTG 1 cut(s) 2147
PsuI RGATCY 2 cut(s) 638, 2246
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 4 cut(s) 77, 498, 2104, 2224
RsaNI GTAC 4 cut(s) 76, 497, 2103, 2223
RseI CAYNNNNRTG 6 cut(s) 422, 510, 796, 1640, 1659, 2294
SalI GTCGAC 1 cut(s) 1829
SaqAI TTAA 4 cut(s) 1593, 1620, 2094, 2172
Sau3AI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
Sau96I GGNCC 3 cut(s) 974, 1493, 1508
ScaI AGTACT 2 cut(s) 2104, 2224
SchI GAGTC 4 cut(s) 304, 459, 624, 644
ScrFI CCNGG 5 cut(s) 528, 1542, 1588, 1854, 1995
SduI GDGCHC 2 cut(s) 406, 1692
SfaNI GCATC 4 cut(s) 218, 307, 1327, 2147
SfcI CTRYAG 5 cut(s) 684, 912, 1419, 1707, 1734
SinI GGWCC 2 cut(s) 974, 1508
SmiMI CAYNNNNRTG 6 cut(s) 422, 510, 796, 1640, 1659, 2294
SmlI CTYRAG 4 cut(s) 177, 271, 292, 1314
SmoI CTYRAG 4 cut(s) 177, 271, 292, 1314
SpeI ACTAGT 1 cut(s) 1132
Sse9I AATT 9 cut(s) 550, 765, 825, 967, 1254, 1860, 1909, 2189, 2233
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 3 cut(s) 690, 1002, 2040
SspI AATATT 1 cut(s) 907
SspMI CTAG 8 cut(s) 48, 1029, 1133, 1488, 1578, 1968, 2100, 2157
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 5 cut(s) 526, 1540, 1586, 1852, 1993
StyI CCWWGG 6 cut(s) 304, 589, 1007, 1159, 1438, 2156
TaaI ACNGT 7 cut(s) 628, 833, 1021, 1447, 1976, 2074, 2149
TaiI ACGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 1830
TaqII GACCGA 1 cut(s) 1056
TasI AATT 9 cut(s) 550, 765, 825, 967, 1254, 1860, 1909, 2189, 2233
TatI WGTACW 2 cut(s) 2102, 2222
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 4 cut(s) 1593, 1620, 2094, 2172
Tru9I TTAA 4 cut(s) 1593, 1620, 2094, 2172
TseFI GTSAC 9 cut(s) 116, 647, 861, 899, 1531, 1598, 1701, 2056, 2074
Tsp45I GTSAC 9 cut(s) 116, 647, 861, 899, 1531, 1598, 1701, 2056, 2074
TspDTI ATGAA 8 cut(s) 25, 636, 909, 1101, 1323, 1473, 2004, 2060
TspGWI ACGGA 3 cut(s) 434, 937, 954
VneI GTGCAC 1 cut(s) 1688
VpaK11BI GGWCC 2 cut(s) 974, 1508
XapI RAATTY 1 cut(s) 967
XceI RCATGY 2 cut(s) 1227, 1829
XmaJI CCTAGG 1 cut(s) 2156
XmiI GTMKAC 2 cut(s) 852, 1830
XspI CTAG 8 cut(s) 48, 1029, 1133, 1488, 1578, 1968, 2100, 2157
ZrmI AGTACT 2 cut(s) 2104, 2224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.