Rh1DG232800

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018279
Physical Location & Seq
Forward (+)
44597253 .. 44600364
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG232800.1

Sequence Viewer

Length: 2310 bp
ATGATGGTTAGAGCATGCTTGTTGTTCTTGGCTTTCATGGCTACAACTAGAACAGCTCTGATGGAAACACAGACGTACATTGTTCACATGGACAAGGCGAAAATCCCTGCCTCGGGTCACAAAGATCAACACTACTATTACAAAGCAGTGATGGATTCCATTACCGAACTCTCATCTCAAGAAGAAGAGGAAGAAGAAGGTCGCAAAGCATCAGCTCCTCAGCTTCTTTACATCTACAACACTGCTATTTCGGGTTTTGCAGCAAAGCTCTCAAGCAATCAACTCAAATCCTTGAGCCAAATCCATGGTTTCCTATATGCCACACCCGATGAGCTTCTAACTCTCCACACCACACACACTCCTCAGTTTCTTGGCTTACAAAAAGGGAAAGGACTTTGGAGTGCTCCCAACTTGGCCTCGGATGTGATCATTGGAGTTGTTGACACCGGAGTCTGGCCAGAACATGTTAGCTTCAAGGACAAAGGAATGTCCCCTGTACCCTCTCGCTGGAACGGCACTTGTGAAGAAGGCAAAAGGTTCTCACACTCAAATTGTAACAAAAAGCTTATTGGTGCAAGAGCCTTTTTCCAAGGGTATGAGGCCATTGTCGGAAGAGTCAATGAAACAGTGGATTATAGATCCCCTCGTGACTCCGAAGGACATGGAACTCACACTGCTTCAACTGCAGGCGGTAATGTTGTCAAACAAGCAAGCTTATTTGGCTTAGCAAAGGGATCGGCCAGTGGAATGAGTCATTCTTCAAGAATTGCAGCCTATAAGGCCTGTTGGCCATCGGGGTGTGCTAACTCTGATGTAATGGCTGCTATTGACAGTGCTGTTTCTGATGGGGTAGACATTTTGTCACTCTCATTGGGAGGTCTTCCGAAACCTTATTTTCGTGACAATATTGCTATAGCTTCATTTGGTGCAGTCCAACATGGAGTTTCCGTGTCATCTTCAGCTGGAAATTCAGGTCCCTTTCGCTCAAGTGTTAGCAATACCGCACCTTGGATCATGACTGTTGCTGCTAGCTACACTGACCGAAGCTTCCCAGTGATAGTGAAGCTTGGGAATGGACAAACTTATGAAGGGTCATCACTATATTCAGGTAAGAAAACTAAGCAGTTGCCACTTGTGTATAACAGAACAGCAGGTGGCCAAGGAGCTGAGCATTGTGTCGATGGCTCGCTTGACACAAAGCTGGTGAAGGGAAAGATTGTAGCATGTGAGCGCGGAACACACAGCCGGACTGAAATTGGAGAGGAAGTGAAGAAAGCAGGAGGAGCCGGAATGCTTCTACTCAATAATGAAGCTCAAGGAGAGGAACTACTTGCTGATGCTCACATTTTGCCAGCCACTTCTCTTGGAGCTTCGGCTTCTAAAGCCGTGAGAAAATACGCAGGTGCAGCAAAGAAACCTACAGCTTTGCTTTTGTTCCAAGGCACGGTCTTTGGTAAAACTGCTCCGGTGATGGCAGCATTTTCATCTAGGGGGCCAAGCTCAGTTGGACCGGACGTGATCAAGCCCGATGTGACTGCTCCTGGTGTCAACATTTTGGCTGCTTGGCCAGCCGAGACTAGCCCAACCAGGCTTAAAAGTGACAAGAGAAAGGTGTTGTTTAACATGGTCTCAGGCACTTCAATGTCTTGCCCTCATGTTAGTGGCTTAGCTGCTCTTATCAAATCAGTGCACAAAGATTGGTCACCTGCAGCAATCAAATCTGCACTTATGACTACAGCTTATACACTGAACAACAAAGGCTCTCCGATTTCTGATTTTGGCTCAAAAAATCAATCAGCTAACCCTTTTGCATTTGGTTCGGGGCATGTCGACCCAGAAAGTGCTGCCGATCCAGGACTAATTTATGATATCACCAGCAAAGATTACTTGCTCTATTTATGTAGCTTGAATTACACATCTTCCCAAATAGCTCTTTTCTCCAGTGGGATTAGTAACTTCACTTGTCCTAGCAACAGTGCAGTTCTTCTTCAGCCCGGCGACTTGAACTACCCTTCATTCTCAGTGGTTTTCAAAAAAGGTGGAATGAAAATGAGTGTGACATACAAGAGGACAGTGACAAATGTTGGTGCCATTAACCCTAGTACTTATGCAGTGCAAGTGGAAGCACCCATTGGAGTATCAGTTACTGTTGAACCTAAGAAGTTGGTTTTTAAGAAGATGGGTGAGAAATTGAGCTACAAGGTGAGTTTTGTTGGAGTGAGTACTAAAACCAATTCCTCATTTGGATCCTTAGTTTGGGTGAGTGAGAAATATAGGGTTAGAAGTCCCATTGCAGTGATTTGGGTGTGA

Protein Analysis

769

Amino Acids

81.81

Weight (kDa)

9.23

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 25 - 116 3e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 140 - 594 3.3e-49 Subtilase family
PA PF02225 375 - 461 1.2e-10 PA domain
fn3_6 PF17766 667 - 766 2.3e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01900
malus_domestica MD02G1196800.v1.1
prunus_persica Prupe.2G152000_v2.0.a1
pyrus_communis pycom02g16010
rosa_chinensis RchiOBHm_Chr1g0351771
rosa_roxburghii Rroxscaffold_4G00303560
rosa_rugosa Rorug01G0221300
rosa_samantha Rh1AG235300 Rh1DG232800
rosa_wichuraiana Rw1G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1142, 1391, 1714
AasI GACNNNNNNGTC 1 cut(s) 449
Acc36I ACCTGC 3 cut(s) 1142, 1391, 1714
AccB1I GGYRCC 1 cut(s) 2087
AccI GTMKAC 2 cut(s) 852, 1830
AccII CGCG 1 cut(s) 1233
AciI CCGC 3 cut(s) 690, 1002, 1233
AclWI GGATC 6 cut(s) 633, 742, 1019, 1844, 2241, 2254
AcoI YGGCCR 5 cut(s) 455, 738, 788, 1156, 1565
AcsI RAATTY 1 cut(s) 967
AcuI CTGAAG 2 cut(s) 942, 1973
AfaI GTAC 4 cut(s) 77, 498, 2104, 2224
AfiI CCNNNNNNNGG 7 cut(s) 112, 113, 453, 507, 1008, 1444, 2256
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 8 cut(s) 475, 681, 762, 1641, 1909, 2005, 2032, 2153
AhdI GACNNNNNGTC 1 cut(s) 859
AjiI CACGTC 1 cut(s) 1516
AjnI CCWGG 3 cut(s) 1540, 1586, 1852
AjuI GAANNNNNNNTTGG 2 cut(s) 2115, 2147
Alw21I GWGCWC 2 cut(s) 406, 1692
Alw26I GTCTC 2 cut(s) 1568, 1633
Alw44I GTGCAC 1 cut(s) 1688
AlwI GGATC 6 cut(s) 633, 742, 1019, 1844, 2241, 2254
AlwNI CAGNNNCTG 1 cut(s) 2147
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 9 cut(s) 414, 455, 600, 738, 780, 788, 1156, 1493, 1565
ApaLI GTGCAC 1 cut(s) 1688
ApoI RAATTY 1 cut(s) 967
AspLEI GCGC 1 cut(s) 1233
AspS9I GGNCC 3 cut(s) 974, 1493, 1508
AsuC2I CCSGG 1 cut(s) 1995
AsuHPI GGTGA 7 cut(s) 1216, 1480, 1695, 1864, 2195, 2215, 2272
AsuNHI GCTAGC 1 cut(s) 1028
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 2 cut(s) 974, 1508
BaeGI GKGCMC 1 cut(s) 1692
BalI TGGCCA 4 cut(s) 457, 790, 1158, 1567
BamHI GGATCC 1 cut(s) 2246
BanI GGYRCC 1 cut(s) 2087
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 1 cut(s) 872
Bbv12I GWGCWC 2 cut(s) 406, 1692
BbvCI CCTCAGC 1 cut(s) 219
BccI CCATC 7 cut(s) 55, 145, 799, 839, 1175, 1465, 2173
BceAI ACGGC 2 cut(s) 529, 1370
BcgI CGANNNNNNTGC 6 cut(s) 717, 751, 1517, 1551, 1800, 1834
BciT130I CCWGG 3 cut(s) 1542, 1588, 1854
BclI TGATCA 2 cut(s) 426, 1518
BcnI CCSGG 1 cut(s) 1995
BcoDI GTCTC 2 cut(s) 1568, 1633
BfaI CTAG 6 cut(s) 48, 1029, 1488, 1578, 1968, 2100
BfmI CTRYAG 5 cut(s) 684, 912, 1419, 1707, 1734
BfuAI ACCTGC 3 cut(s) 1142, 1391, 1714
BglI GCCNNNNNGGC 1 cut(s) 779
BlpI GCTNAGC 3 cut(s) 724, 1167, 1666
BmcAI AGTACT 2 cut(s) 2104, 2224
Bme1390I CCNGG 4 cut(s) 1542, 1588, 1854, 1995
Bme18I GGWCC 2 cut(s) 974, 1508
BmeRI GACNNNNNGTC 1 cut(s) 859
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 1516
BmgT120I GGNCC 3 cut(s) 974, 1493, 1508
BmiI GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
BmrFI CCNGG 4 cut(s) 1542, 1588, 1854, 1995
BmrI ACTGGG 1 cut(s) 1046
BmsI GCATC 2 cut(s) 218, 1327
BmtI GCTAGC 1 cut(s) 1032
BmuI ACTGGG 1 cut(s) 1046
BpiI GAAGAC 1 cut(s) 872
BpmI CTGGAG 1 cut(s) 1924
Bpu10I CCTNAGC 1 cut(s) 219
Bpu1102I GCTNAGC 3 cut(s) 724, 1167, 1666
BpuEI CTTGAG 5 cut(s) 162, 256, 313, 970, 1299
BpuMI CCSGG 1 cut(s) 1995
BsaI GGTCTC 1 cut(s) 1633
BsaJI CCNNGG 7 cut(s) 111, 304, 417, 589, 1007, 1159, 1438
BsaWI WCCGGW 3 cut(s) 446, 1465, 1510
BsaXI ACNNNNNCTCC 8 cut(s) 343, 373, 933, 963, 1251, 1281, 2127, 2157
Bsc4I CCNNNNNNNGG 7 cut(s) 112, 113, 453, 507, 1008, 1444, 2256
Bse1I ACTGG 3 cut(s) 741, 1052, 1941
Bse3DI GCAATG 1 cut(s) 2289
BseBI CCWGG 3 cut(s) 1542, 1588, 1854
BseDI CCNNGG 7 cut(s) 111, 304, 417, 589, 1007, 1159, 1438
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 7 cut(s) 112, 113, 453, 507, 1008, 1444, 2256
BseMI GCAATG 1 cut(s) 2289
BseMII CTCAG 6 cut(s) 233, 377, 1158, 1515, 1644, 2034
BseNI ACTGG 3 cut(s) 741, 1052, 1941
BseRI GAGGAG 3 cut(s) 207, 351, 1296
BseSI GKGCMC 1 cut(s) 1692
BsgI GTGCAG 4 cut(s) 948, 1425, 1707, 1998
Bsh1236I CGCG 1 cut(s) 1233
BshFI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
BshNI GGYRCC 1 cut(s) 2087
BsiHKAI GWGCWC 2 cut(s) 406, 1692
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 6 cut(s) 447, 1246, 1287, 1466, 1511, 1995
BslFI GGGAC 3 cut(s) 475, 960, 2271
BslI CCNNNNNNNGG 7 cut(s) 112, 113, 453, 507, 1008, 1444, 2256
BsmAI GTCTC 2 cut(s) 1568, 1633
BsmFI GGGAC 3 cut(s) 475, 960, 2271
BsmI GAATGC 1 cut(s) 1296
BsnI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
Bso31I GGTCTC 1 cut(s) 1633
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 2 cut(s) 406, 1692
Bsp143I GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
Bsp1720I GCTNAGC 3 cut(s) 724, 1167, 1666
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 3 cut(s) 690, 1002, 1233
BspANI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
BspCNI CTCAG 6 cut(s) 232, 376, 1159, 1514, 1643, 2033
BspFNI CGCG 1 cut(s) 1233
BspHI TCATGA 1 cut(s) 1014
BspLI GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
BspMAI CTGCAG 2 cut(s) 688, 1711
BspMI ACCTGC 3 cut(s) 1142, 1391, 1714
BspOI GCTAGC 1 cut(s) 1032
BspPI GGATC 6 cut(s) 633, 742, 1019, 1844, 2241, 2254
BspT107I GGYRCC 1 cut(s) 2087
BspTNI GGTCTC 1 cut(s) 1633
BsrDI GCAATG 1 cut(s) 2289
BsrI ACTGG 3 cut(s) 741, 1052, 1941
BssECI CCNNGG 7 cut(s) 111, 304, 417, 589, 1007, 1159, 1438
BssMI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 5 cut(s) 304, 589, 1007, 1159, 1438
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 3 cut(s) 1542, 1588, 1854
Bst4CI ACNGT 7 cut(s) 628, 833, 1021, 1447, 1976, 2074, 2149
Bst6I CTCTTC 2 cut(s) 180, 607
BstC8I GCNNGC 7 cut(s) 16, 688, 712, 1030, 1187, 1353, 1569
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 1701
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 1233
BstHHI GCGC 1 cut(s) 1233
BstKTI GATC 8 cut(s) 127, 429, 641, 737, 1014, 1521, 1852, 2249
BstMAI GTCTC 2 cut(s) 1568, 1633
BstMBI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
BstMWI GCNNNNNNNGC 9 cut(s) 38, 513, 683, 720, 779, 1283, 1382, 1406, 1568
BstNI CCWGG 3 cut(s) 1542, 1588, 1854
BstNSI RCATGY 4 cut(s) 18, 467, 1227, 1829
BstPI GGTNACC 1 cut(s) 1701
BstSCI CCNGG 4 cut(s) 1540, 1586, 1852, 1993
BstSFI CTRYAG 5 cut(s) 684, 912, 1419, 1707, 1734
BstSLI GKGCMC 1 cut(s) 1692
BstUI CGCG 1 cut(s) 1233
BstV2I GAAGAC 1 cut(s) 872
BstX2I RGATCY 2 cut(s) 638, 2246
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 2 cut(s) 638, 2246
BsuRI GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
BtgI CCRYGG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 1516
BtsCI GGATG 1 cut(s) 427
BtsI GCAGTG 5 cut(s) 153, 240, 672, 2118, 2301
BveI ACCTGC 3 cut(s) 1142, 1391, 1714
Cac8I GCNNGC 7 cut(s) 16, 688, 712, 1030, 1187, 1353, 1569
CaiI CAGNNNCTG 1 cut(s) 2147
CciI TCATGA 1 cut(s) 1014
CfoI GCGC 1 cut(s) 1233
Cfr13I GGNCC 3 cut(s) 974, 1493, 1508
Csp6I GTAC 4 cut(s) 76, 497, 2103, 2223
CspCI CAANNNNNGTGG 4 cut(s) 1345, 1380, 2020, 2055
CviQI GTAC 4 cut(s) 76, 497, 2103, 2223
DpnI GATC 8 cut(s) 126, 428, 640, 736, 1013, 1520, 1851, 2248
DpnII GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
DrdI GACNNNNNNGTC 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 859
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 5 cut(s) 455, 738, 788, 1156, 1565
Eam1104I CTCTTC 2 cut(s) 180, 607
Eam1105I GACNNNNNGTC 1 cut(s) 859
EarI CTCTTC 2 cut(s) 180, 607
Eco130I CCWWGG 5 cut(s) 304, 589, 1007, 1159, 1438
Eco147I AGGCCT 1 cut(s) 782
Eco31I GGTCTC 1 cut(s) 1633
Eco32I GATATC 1 cut(s) 1870
Eco47I GGWCC 2 cut(s) 974, 1508
Eco57I CTGAAG 2 cut(s) 942, 1973
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 1701
EcoO109I RGGNCCY 1 cut(s) 974
EcoO65I GGTNACC 1 cut(s) 1701
EcoRII CCWGG 3 cut(s) 1540, 1586, 1852
EcoRV GATATC 1 cut(s) 1870
EcoT14I CCWWGG 5 cut(s) 304, 589, 1007, 1159, 1438
ErhI CCWWGG 5 cut(s) 304, 589, 1007, 1159, 1438
FaqI GGGAC 3 cut(s) 475, 960, 2271
FbaI TGATCA 2 cut(s) 426, 1518
FblI GTMKAC 2 cut(s) 852, 1830
FokI GGATG 1 cut(s) 434
FspBI CTAG 6 cut(s) 48, 1029, 1488, 1578, 1968, 2100
GlaI GCGC 1 cut(s) 1232
GsuI CTGGAG 1 cut(s) 1924
HaeIII GGCC 9 cut(s) 416, 457, 602, 740, 782, 790, 1158, 1495, 1567
HapII CCGG 6 cut(s) 447, 1246, 1287, 1466, 1511, 1995
HhaI GCGC 1 cut(s) 1233
Hin6I GCGC 1 cut(s) 1231
HinP1I GCGC 1 cut(s) 1231
HincII GTYRAC 3 cut(s) 442, 1549, 1831
HindII GTYRAC 3 cut(s) 442, 1549, 1831
HindIII AAGCTT 4 cut(s) 563, 712, 1045, 1064
HinfI GANTC 5 cut(s) 155, 450, 615, 650, 751
HpaII CCGG 6 cut(s) 447, 1246, 1287, 1466, 1511, 1995
HphI GGTGA 7 cut(s) 1216, 1480, 1695, 1864, 2195, 2215, 2272
Hpy166II GTNNAC 6 cut(s) 85, 442, 853, 1549, 1690, 1831
Hpy188I TCNGA 9 cut(s) 60, 421, 611, 655, 811, 844, 885, 1767, 1774
Hpy188III TCNNGA 5 cut(s) 179, 647, 762, 899, 1015
Hpy8I GTNNAC 6 cut(s) 85, 442, 853, 1549, 1690, 1831
HpyAV CCTTC 6 cut(s) 191, 521, 650, 1082, 1201, 2022
HpyCH4III ACNGT 7 cut(s) 628, 833, 1021, 1447, 1976, 2074, 2149
HpyCH4IV ACGT 2 cut(s) 74, 1515
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 513, 683, 720, 779, 1283, 1382, 1406, 1568
HpySE526I ACGT 2 cut(s) 74, 1515
HspAI GCGC 1 cut(s) 1231
Ksp22I TGATCA 2 cut(s) 426, 1518
Kzo9I GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
LmnI GCTCC 7 cut(s) 220, 409, 1163, 1283, 1367, 1468, 1543
LweI GCATC 2 cut(s) 218, 1327
MaeI CTAG 6 cut(s) 48, 1029, 1488, 1578, 1968, 2100
MaeII ACGT 2 cut(s) 74, 1515
MalI GATC 8 cut(s) 126, 428, 640, 736, 1013, 1520, 1851, 2248
MboI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
MflI RGATCY 2 cut(s) 638, 2246
MhlI GDGCHC 2 cut(s) 406, 1692
MlsI TGGCCA 4 cut(s) 457, 790, 1158, 1567
MluCI AATT 8 cut(s) 550, 765, 967, 1254, 1860, 1909, 2189, 2233
MluNI TGGCCA 4 cut(s) 457, 790, 1158, 1567
MlyI GAGTC 4 cut(s) 459, 624, 644, 760
MmeI TCCRAC 4 cut(s) 589, 958, 1486, 2194
Mox20I TGGCCA 4 cut(s) 457, 790, 1158, 1567
MscI TGGCCA 4 cut(s) 457, 790, 1158, 1567
MseI TTAA 4 cut(s) 1593, 1620, 2094, 2172
MslI CAYNNNNRTG 4 cut(s) 796, 1640, 1659, 2294
Msp20I TGGCCA 4 cut(s) 457, 790, 1158, 1567
MspA1I CMGCKG 1 cut(s) 962
MspI CCGG 6 cut(s) 447, 1246, 1287, 1466, 1511, 1995
MspR9I CCNGG 4 cut(s) 1542, 1588, 1854, 1995
Mva1269I GAATGC 1 cut(s) 1296
MvaI CCWGG 3 cut(s) 1542, 1588, 1854
MvnI CGCG 1 cut(s) 1233
MwoI GCNNNNNNNGC 9 cut(s) 38, 513, 683, 720, 779, 1283, 1382, 1406, 1568
NciI CCSGG 1 cut(s) 1995
NcoI CCATGG 1 cut(s) 304
NdeII GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
NheI GCTAGC 1 cut(s) 1028
NlaIV GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
NmeAIII GCCGAG 1 cut(s) 1597
NmuCI GTSAC 9 cut(s) 116, 647, 861, 899, 1531, 1598, 1701, 2056, 2074
NspI RCATGY 4 cut(s) 18, 467, 1227, 1829
PaeI GCATGC 1 cut(s) 18
PagI TCATGA 1 cut(s) 1014
PaqCI CACCTGC 3 cut(s) 1142, 1391, 1714
PceI AGGCCT 1 cut(s) 782
PciI ACATGT 1 cut(s) 463
PctI GAATGC 1 cut(s) 1296
PfeI GAWTC 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 1852
PleI GAGTC 4 cut(s) 458, 623, 644, 759
PpsI GAGTC 4 cut(s) 458, 623, 644, 759
PpuMI RGGWCCY 1 cut(s) 974
PscI ACATGT 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 974
Psp6I CCWGG 3 cut(s) 1540, 1586, 1852
PspEI GGTNACC 1 cut(s) 1701
PspGI CCWGG 3 cut(s) 1540, 1586, 1852
PspN4I GGNNCC 5 cut(s) 976, 1285, 1494, 2089, 2248
PspPI GGNCC 3 cut(s) 974, 1493, 1508
PspPPI RGGWCCY 1 cut(s) 974
PstI CTGCAG 2 cut(s) 688, 1711
PstNI CAGNNNCTG 1 cut(s) 2147
PsuI RGATCY 2 cut(s) 638, 2246
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 4 cut(s) 77, 498, 2104, 2224
RsaNI GTAC 4 cut(s) 76, 497, 2103, 2223
RseI CAYNNNNRTG 4 cut(s) 796, 1640, 1659, 2294
SalI GTCGAC 1 cut(s) 1829
SaqAI TTAA 4 cut(s) 1593, 1620, 2094, 2172
Sau3AI GATC 8 cut(s) 124, 426, 638, 734, 1011, 1518, 1849, 2246
Sau96I GGNCC 3 cut(s) 974, 1493, 1508
ScaI AGTACT 2 cut(s) 2104, 2224
SchI GAGTC 4 cut(s) 459, 624, 644, 760
ScrFI CCNGG 4 cut(s) 1542, 1588, 1854, 1995
SduI GDGCHC 2 cut(s) 406, 1692
SfaNI GCATC 2 cut(s) 218, 1327
SfcI CTRYAG 5 cut(s) 684, 912, 1419, 1707, 1734
SinI GGWCC 2 cut(s) 974, 1508
SmiMI CAYNNNNRTG 4 cut(s) 796, 1640, 1659, 2294
SmlI CTYRAG 5 cut(s) 177, 271, 292, 985, 1314
SmoI CTYRAG 5 cut(s) 177, 271, 292, 985, 1314
SphI GCATGC 1 cut(s) 18
Sse9I AATT 8 cut(s) 550, 765, 967, 1254, 1860, 1909, 2189, 2233
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 3 cut(s) 690, 1002, 1233
SspI AATATT 1 cut(s) 907
SspMI CTAG 6 cut(s) 48, 1029, 1488, 1578, 1968, 2100
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 4 cut(s) 1540, 1586, 1852, 1993
StyI CCWWGG 5 cut(s) 304, 589, 1007, 1159, 1438
TaaI ACNGT 7 cut(s) 628, 833, 1021, 1447, 1976, 2074, 2149
TaiI ACGT 2 cut(s) 77, 1518
TaqI TCGA 2 cut(s) 1179, 1830
TaqII GACCGA 1 cut(s) 1056
TasI AATT 8 cut(s) 550, 765, 967, 1254, 1860, 1909, 2189, 2233
TatI WGTACW 2 cut(s) 2102, 2222
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 4 cut(s) 1593, 1620, 2094, 2172
Tru9I TTAA 4 cut(s) 1593, 1620, 2094, 2172
TseFI GTSAC 9 cut(s) 116, 647, 861, 899, 1531, 1598, 1701, 2056, 2074
Tsp45I GTSAC 9 cut(s) 116, 647, 861, 899, 1531, 1598, 1701, 2056, 2074
TspDTI ATGAA 8 cut(s) 25, 636, 909, 1101, 1323, 1473, 2004, 2060
TspGWI ACGGA 1 cut(s) 937
VneI GTGCAC 1 cut(s) 1688
VpaK11BI GGWCC 2 cut(s) 974, 1508
XapI RAATTY 1 cut(s) 967
XceI RCATGY 4 cut(s) 18, 467, 1227, 1829
XmiI GTMKAC 2 cut(s) 852, 1830
XspI CTAG 6 cut(s) 48, 1029, 1488, 1578, 1968, 2100
ZrmI AGTACT 2 cut(s) 2104, 2224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.