MD02G1197100.v1.1

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0018503
Physical Location & Seq
Forward (+)
19014867 .. 19015181
315 bp
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UTR
Exon/CDS
Intron
MD02G1197100.v1.1.491

Sequence Viewer

Length: 315 bp
ATGATTAACAAGAAAGTCTCATTGGCATTGGGAAGAGATGGGGTGACTGTTAATGTAAGGTGTGGAGCTATTATCGATGAGGAGGAAATGGTGCAGTGTTTGGTGCAAGGAGAGATCAAGGGTGCTGGCTTAGATGTATTTGAGAATGAGTCTGAAGTTCTGAAAGCGCTCTTTGGACTCGATAATGTTGTCTTGTCACCGCATCAAGCTGATACGATAGAAGACTATTTCAAGGATTTGAGTGATCTAGCAGTAGCGAATTTGGAGGCATTCTTCTCAAATAAACCATTGCTTTCTGAAGGGGTGAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

105

Amino Acids

11.28

Weight (kDa)

4.27

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 1 - 70 2.3e-13 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018503)

Species Orthologous Gene IDs
malus_domestica MD02G1197100.v1.1
prunus_persica Prupe.2G150600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0351541 RchiOBHm_Chr5g0049221
rosa_multiflora Rmu_co8043636.1_g000001 Rmu_co8344733.1_g000001 Rmu_sc0003804.1_g000015
rosa_roxburghii Rroxscaffold_5G00377260
rosa_samantha Rh2BG413500
rosa_wichuraiana Rw0G020780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 200
AcsI RAATTY 1 cut(s) 259
AcuI CTGAAG 1 cut(s) 174
AfeI AGCGCT 1 cut(s) 168
AgsI TTSAA 1 cut(s) 232
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 2 cut(s) 68, 209
AluI AGCT 2 cut(s) 68, 209
Alw26I GTCTC 1 cut(s) 22
Aor51HI AGCGCT 1 cut(s) 168
ApoI RAATTY 1 cut(s) 259
AspLEI GCGC 1 cut(s) 169
AsuHPI GGTGA 2 cut(s) 55, 189
BbsI GAAGAC 1 cut(s) 228
BccI CCATC 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 1 cut(s) 248
BfoI RGCGCY 1 cut(s) 170
BmsI GCATC 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 228
Bsa29I ATCGAT 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 287
BseCI ATCGAT 1 cut(s) 75
BseMI GCAATG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 269
Bsp143I GATC 2 cut(s) 114, 244
BspACI CCGC 1 cut(s) 200
BspDI ATCGAT 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 287
BssMI GATC 2 cut(s) 114, 244
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 1 cut(s) 130
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 2 cut(s) 117, 247
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 2 cut(s) 114, 244
BstV2I GAAGAC 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 75
BsuTUI ATCGAT 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 127
CfoI GCGC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 75
CviJI RGCY 3 cut(s) 68, 129, 209
CviKI_1 RGCY 3 cut(s) 68, 129, 209
DdeI CTNAG 1 cut(s) 130
DpnI GATC 2 cut(s) 116, 246
DpnII GATC 2 cut(s) 114, 244
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco47III AGCGCT 1 cut(s) 168
Eco57I CTGAAG 1 cut(s) 174
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 170
HhaI GCGC 1 cut(s) 169
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 2 cut(s) 149, 177
HphI GGTGA 2 cut(s) 55, 189
Hpy188I TCNGA 3 cut(s) 154, 162, 298
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 2 cut(s) 94, 106
HpyF3I CTNAG 1 cut(s) 130
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 2 cut(s) 114, 244
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 1 cut(s) 111
LweI GCATC 1 cut(s) 211
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 43, 195
MalI GATC 2 cut(s) 116, 246
MboI GATC 2 cut(s) 114, 244
MboII GAAGA 3 cut(s) 45, 233, 265
MluCI AATT 1 cut(s) 259
MlyI GAGTC 2 cut(s) 158, 171
MnlI CCTC 3 cut(s) 73, 76, 259
MseI TTAA 2 cut(s) 6, 51
Mva1269I GAATGC 1 cut(s) 269
NdeII GATC 2 cut(s) 114, 244
NmuCI GTSAC 2 cut(s) 43, 195
PctI GAATGC 1 cut(s) 269
PleI GAGTC 2 cut(s) 157, 171
PpsI GAGTC 2 cut(s) 157, 171
SaqAI TTAA 2 cut(s) 6, 51
Sau3AI GATC 2 cut(s) 114, 244
SchI GAGTC 2 cut(s) 158, 171
SetI ASST 3 cut(s) 62, 70, 211
SfaNI GCATC 1 cut(s) 211
SgeI CNNG 9 cut(s) 22, 119, 130, 138, 191, 205, 218, 244, 260
Sse9I AATT 1 cut(s) 259
SsiI CCGC 1 cut(s) 200
SspMI CTAG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 2 cut(s) 75, 180
TasI AATT 1 cut(s) 259
Tru1I TTAA 2 cut(s) 6, 51
Tru9I TTAA 2 cut(s) 6, 51
TscAI CASTG 1 cut(s) 101
TseFI GTSAC 2 cut(s) 43, 195
Tsp45I GTSAC 2 cut(s) 43, 195
TspRI CASTG 1 cut(s) 101
XapI RAATTY 1 cut(s) 259
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.