MD02G1256300.v1.1
ERF Family

Performs the first committed step in the biosynthesis of isoprene-containing compounds such as sterols and terpenoids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010940
Physical Location & Seq
Reverse (-)
30854526 .. 30859747
5222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1256300.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGGCCGACGAGTCGCGGAAATGGGTGCTGATGGTGACGGCTCAGACGCCCACCAACATCGCCGTGATCAAGTACTGGGGGAAGAGGGACGAGAAGCTCATCTTGCCAGTCAATGACAGCATCAGCGTGACGCTTGATCCTGCGCACCTTTGCACCACCACCACTGTCTCCGTCAGTCCCACCTTTGACCAAGATCGGATGTGGCTCAATGGCGAGGAGATTTCCATTTCTGGAGGCAGATTCCAAAGCTGTTTAAGAGAAATTCGCAGTCGAGCTACCGATGTTGATGATAAGGAAAAGGGAATCAAGATTACTAAGAAGGATTGGGAAAACTTGCATGTACATATTTCTTCATATAACAATTTTCCTACTGCTGCTGGATTGGCTTCCTCAGCAGCTGGTTTTGCTTGTCTAGTTTTTGCACTCGGAAAGTTAATGAATGTGAAAGAAGATCACAGCCAGCTTTCTGCTATTGCAAGGCAAGGGTCTGGTAGTGCTTGCCGCAGCTTATATGGGGGATTTGTCAAGTGGATTATGGGAAAAGATGAGAAGGGAACCGACAGTCTTGCAGTCCAAGTTGCAGATGAGAAGCACTGGGATGAGCTTGTTATTGTTATCGCTGTGGTAAGTTCACGGCAGAAGGAAACAAGCAGCACTACAGGAATGCGTGATACTGTTGAAACAAGTTTGCTCTTACAACATAGAGCAAAAGAAATAGTACCAAAACGCATATTAAAAATGGAAGAAGCCATAAAAAATCGTGATTTTGCTTCTTTTGCACAATTGACCTGTACTGACAGTAACCAGTTTCATGCTGTCTGCCTGGACACATGTCCACCCATTTTCTACATGAATGATACATCCCACAGGATAATAAGCCTTGTTGAAAAGTGGAACAAAGCAGAAGGAACACCTCAGGTGGCTTATACATTTGATGCGGGGCCAAATGCGGTTCTCATTGCACGTAACAGAAAAACAGCTGCCCTTTTGGTTCAGAAGTTGCTATTCTGCTTTCCTCCAAATTCTGATGCAGATTTGAACAGTTTCGTTCTTGGTGATAAGTCAATTCTAAAAGATGCCGGTGTTGAGAGCCCCGAGGATATTGAAACTTTACCCCCACCTCCAGAAATCAAGGATCCGTCCCAGAAATTTCGAGGAGATGTCAGTTACTTTATTTGCACAAGACCTGGGAGAGGTCCAGTTGTGCTGTCAGATGAAAGTCATCTTCTCAACCCTGAAACTGGATTGCCGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004163 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006066 GO:0006084 GO:0006139 GO:0006163 GO:0006464 GO:0006486 GO:0006487 GO:0006488 GO:0006489 GO:0006490 GO:0006629 GO:0006637 GO:0006644 GO:0006694 GO:0006695 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008202 GO:0008203 GO:0008284 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009889 GO:0009987 GO:0016125 GO:0016126 GO:0016829 GO:0016830 GO:0016831 GO:0019216 GO:0019218 GO:0019222 GO:0019287 GO:0019538 GO:0019637 GO:0019693 GO:0030544 GO:0031072 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0034645 GO:0035383 GO:0036211 GO:0042127 GO:0042579 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045540 GO:0046165 GO:0046465 GO:0046483 GO:0046490 GO:0046890 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050810 GO:0051186 GO:0055086 GO:0062012 GO:0065007 GO:0070085 GO:0071704 GO:0072521 GO:0080090 GO:0090181 GO:0090407 GO:0106118 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902652 GO:1902653 GO:1902930
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
GHMP_kinases_N

Protein Analysis

419

Amino Acids

46.2

Weight (kDa)

6.18

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MVD-like_N PF22700 17 - 190 1.2e-65 Diphosphomevalonate decarboxylase-like N-terminal domain
GHMP_kinases_N PF00288 105 - 166 2.6e-06 GHMP kinases N terminal domain
MDD_C PF18376 204 - 402 7.7e-66 Mevalonate 5-diphosphate decarboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38700 AT3G54250
fragaria_vesca FvH4_7g05610 FvH4_7g05610
malus_domestica MD02G1256300.v1.1 MD07G1064100.v1.1
prunus_persica Prupe.2G078900_v2.0.a1
pyrus_communis pycom02g21690 pycom07g04970
rosa_chinensis RchiOBHm_Chr1g0332421
rosa_laevigata RLG00000029671
rosa_multiflora Rmu_sc0016943.1_g000003 Rmu_ssc0000161.1_g000007
rosa_roxburghii Rroxscaffold_4G00318190
rosa_rugosa Rorug01G0100700
rosa_samantha Rh1AG124800 Rh1BG095200 Rh1CG118900 Rh1DG129700
rosa_wichuraiana Rw1G010140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 144
AccII CGCG 1 cut(s) 16
AciI CCGC 4 cut(s) 16, 502, 938, 950
AclWI GGATC 3 cut(s) 131, 1130, 1143
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 261, 1021, 1148
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 4 cut(s) 74, 342, 720, 793
AfiI CCNNNNNNNGG 2 cut(s) 1193, 1241
AflIII ACRYGT 1 cut(s) 830
AgsI TTSAA 4 cut(s) 680, 887, 1039, 1106
AjnI CCWGG 2 cut(s) 822, 1186
AluBI AGCT 8 cut(s) 97, 249, 275, 398, 463, 507, 604, 980
AluI AGCT 8 cut(s) 97, 249, 275, 398, 463, 507, 604, 980
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 3 cut(s) 131, 1130, 1143
AlwNI CAGNNNCTG 1 cut(s) 398
Ama87I CYCGRG 1 cut(s) 1094
AoxI GGCC 2 cut(s) 3, 941
ApeKI GCWGC 5 cut(s) 374, 395, 504, 651, 980
ApoI RAATTY 3 cut(s) 261, 1021, 1148
Asp700I GAANNNNTTC 1 cut(s) 1043
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 941, 1196
AsuHPI GGTGA 2 cut(s) 46, 1067
AvaI CYCGRG 1 cut(s) 1094
AvaII GGWCC 1 cut(s) 1196
AxyI CCTNAGG 1 cut(s) 915
BamHI GGATCC 1 cut(s) 1135
BanII GRGCYC 1 cut(s) 1094
BbvCI CCTCAGC 1 cut(s) 391
BbvI GCAGC 5 cut(s) 361, 407, 516, 663, 967
BccI CCATC 1 cut(s) 25
BceAI ACGGC 3 cut(s) 47, 54, 650
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 2 cut(s) 824, 1188
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 657
BisI GCNGC 6 cut(s) 375, 396, 502, 505, 652, 981
BlsI GCNGC 6 cut(s) 376, 397, 503, 506, 653, 982
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 2 cut(s) 824, 1188
Bme18I GGWCC 1 cut(s) 1196
BmeT110I CYCGRG 1 cut(s) 1094
BmgT120I GGNCC 2 cut(s) 941, 1196
BmiI GGNNCC 3 cut(s) 556, 942, 1137
BmrFI CCNGG 2 cut(s) 824, 1188
BmrI ACTGGG 2 cut(s) 85, 604
BmsI GCATC 4 cut(s) 129, 925, 1018, 1066
BmuI ACTGGG 2 cut(s) 85, 604
BoxI GACNNNNGTC 1 cut(s) 831
BpmI CTGGAG 2 cut(s) 252, 1107
Bpu10I CCTNAGC 1 cut(s) 391
BsaAI YACGTR 1 cut(s) 965
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 1095, 1187
Bsc4I CCNNNNNNNGG 2 cut(s) 1193, 1241
Bse118I RCCGGY 1 cut(s) 1079
Bse1I ACTGG 6 cut(s) 80, 107, 599, 805, 1199, 1246
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 957
BseBI CCWGG 2 cut(s) 824, 1188
BseDI CCNNGG 2 cut(s) 1095, 1187
BseGI GGATG 3 cut(s) 204, 604, 860
BseLI CCNNNNNNNGG 2 cut(s) 1193, 1241
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 3 cut(s) 56, 405, 929
BseNI ACTGG 6 cut(s) 80, 107, 599, 805, 1199, 1246
BseRI GAGGAG 2 cut(s) 230, 1170
BseXI GCAGC 5 cut(s) 361, 407, 516, 663, 967
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 2 cut(s) 5, 943
BsiHKCI CYCGRG 1 cut(s) 1094
BsiSI CCGG 1 cut(s) 1080
BslFI GGGAC 3 cut(s) 101, 162, 1126
BslI CCNNNNNNNGG 2 cut(s) 1193, 1241
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 3 cut(s) 101, 162, 1126
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 2 cut(s) 5, 943
BsoBI CYCGRG 1 cut(s) 1094
Bsp1286I GDGCHC 1 cut(s) 1094
Bsp1407I TGTACA 1 cut(s) 340
Bsp143I GATC 5 cut(s) 66, 136, 193, 451, 1135
BspACI CCGC 4 cut(s) 16, 502, 938, 950
BspANI GGCC 2 cut(s) 5, 943
BspCNI CTCAG 3 cut(s) 55, 404, 928
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 3 cut(s) 556, 942, 1137
BspPI GGATC 3 cut(s) 131, 1130, 1143
BsrDI GCAATG 1 cut(s) 957
BsrFI RCCGGY 1 cut(s) 1079
BsrGI TGTACA 1 cut(s) 340
BsrI ACTGG 6 cut(s) 80, 107, 599, 805, 1199, 1246
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 2 cut(s) 1095, 1187
BssMI GATC 5 cut(s) 66, 136, 193, 451, 1135
BssNI GRCGYC 1 cut(s) 47
Bst2UI CCWGG 2 cut(s) 824, 1188
Bst4CI ACNGT 5 cut(s) 166, 563, 676, 800, 1043
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 340
BstBAI YACGTR 1 cut(s) 965
BstC8I GCNNGC 2 cut(s) 461, 499
BstDEI CTNAG 4 cut(s) 42, 315, 391, 915
BstENI CCTNNNNNAGG 1 cut(s) 1191
BstF5I GGATG 3 cut(s) 204, 604, 860
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 5 cut(s) 69, 139, 196, 454, 1138
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 5 cut(s) 66, 136, 193, 451, 1135
BstMWI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 776
BstNI CCWGG 2 cut(s) 824, 1188
BstNSI RCATGY 2 cut(s) 341, 834
BstPAI GACNNNNGTC 1 cut(s) 831
BstSCI CCNGG 2 cut(s) 822, 1186
BstSFI CTRYAG 1 cut(s) 657
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 5 cut(s) 361, 407, 516, 663, 967
BstX2I RGATCY 1 cut(s) 1135
BstYI RGATCY 1 cut(s) 1135
Bsu36I CCTNAGG 1 cut(s) 915
BsuRI GGCC 2 cut(s) 5, 943
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 3 cut(s) 204, 604, 860
BtsIMutI CAGTG 2 cut(s) 162, 592
Cac8I GCNNGC 2 cut(s) 461, 499
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 2 cut(s) 941, 1196
CseI GACGC 2 cut(s) 55, 139
Csp6I GTAC 4 cut(s) 73, 341, 719, 792
CviAII CATG 4 cut(s) 338, 812, 831, 850
CviQI GTAC 4 cut(s) 73, 341, 719, 792
DdeI CTNAG 4 cut(s) 42, 315, 391, 915
DpnI GATC 5 cut(s) 68, 138, 195, 453, 1137
DpnII GATC 5 cut(s) 66, 136, 193, 451, 1135
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 1094
Eco47I GGWCC 1 cut(s) 1196
Eco81I CCTNAGG 1 cut(s) 915
Eco88I CYCGRG 1 cut(s) 1094
EcoNI CCTNNNNNAGG 1 cut(s) 1191
EcoRII CCWGG 2 cut(s) 822, 1186
EcoT38I GRGCYC 1 cut(s) 1094
FaeI CATG 4 cut(s) 341, 815, 834, 853
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 3 cut(s) 101, 162, 1126
FatI CATG 4 cut(s) 337, 811, 830, 849
FauI CCCGC 1 cut(s) 931
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 375, 396, 502, 505, 652, 981
FokI GGATG 3 cut(s) 211, 611, 847
FriOI GRGCYC 1 cut(s) 1094
Fsp4HI GCNGC 6 cut(s) 375, 396, 502, 505, 652, 981
FspBI CTAG 1 cut(s) 413
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 1 cut(s) 144
GluI GCNGC 6 cut(s) 375, 396, 502, 505, 652, 981
GsuI CTGGAG 2 cut(s) 252, 1107
HaeIII GGCC 2 cut(s) 5, 943
HapII CCGG 1 cut(s) 1080
HgaI GACGC 2 cut(s) 55, 139
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 4 cut(s) 341, 815, 834, 853
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HinfI GANTC 3 cut(s) 11, 240, 303
HpaII CCGG 1 cut(s) 1080
HphI GGTGA 2 cut(s) 46, 1067
Hpy166II GTNNAC 2 cut(s) 632, 836
Hpy188I TCNGA 6 cut(s) 45, 198, 428, 996, 1027, 1213
Hpy188III TCNNGA 4 cut(s) 231, 307, 761, 1124
Hpy8I GTNNAC 2 cut(s) 632, 836
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 313, 544, 634, 899
HpyCH4III ACNGT 5 cut(s) 166, 563, 676, 800, 1043
HpyCH4IV ACGT 1 cut(s) 964
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 776
HpyF3I CTNAG 4 cut(s) 42, 315, 391, 915
HpySE526I ACGT 1 cut(s) 964
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 4 cut(s) 341, 815, 834, 853
HspAI GCGC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 5 cut(s) 66, 136, 193, 451, 1135
Lsp1109I GCAGC 5 cut(s) 361, 407, 516, 663, 967
LweI GCATC 4 cut(s) 129, 925, 1018, 1066
MaeI CTAG 1 cut(s) 413
MaeII ACGT 1 cut(s) 964
MaeIII GTNAC 5 cut(s) 34, 127, 800, 965, 1166
MalI GATC 5 cut(s) 68, 138, 195, 453, 1137
MboI GATC 5 cut(s) 66, 136, 193, 451, 1135
MboII GAAGA 5 cut(s) 94, 342, 461, 755, 1217
MfeI CAATTG 1 cut(s) 782
MflI RGATCY 1 cut(s) 1135
MhlI GDGCHC 1 cut(s) 1094
MluCI AATT 6 cut(s) 261, 361, 782, 1021, 1065, 1148
MlyI GAGTC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 1043
MseI TTAA 3 cut(s) 254, 434, 734
MslI CAYNNNNRTG 3 cut(s) 62, 125, 597
MspA1I CMGCKG 2 cut(s) 398, 980
MspI CCGG 1 cut(s) 1080
MspR9I CCNGG 2 cut(s) 824, 1188
MunI CAATTG 1 cut(s) 782
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 2 cut(s) 824, 1188
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 776
NdeII GATC 5 cut(s) 66, 136, 193, 451, 1135
NlaIII CATG 4 cut(s) 341, 815, 834, 853
NlaIV GGNNCC 3 cut(s) 556, 942, 1137
NmuCI GTSAC 2 cut(s) 34, 127
NsbI TGCGCA 1 cut(s) 144
NspI RCATGY 2 cut(s) 341, 834
PciI ACATGT 1 cut(s) 830
PctI GAATGC 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 1043
PfeI GAWTC 2 cut(s) 240, 303
PkrI GCNGC 6 cut(s) 376, 397, 503, 506, 653, 982
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 965
PscI ACATGT 1 cut(s) 830
PshAI GACNNNNGTC 1 cut(s) 831
Psp6I CCWGG 2 cut(s) 822, 1186
PspGI CCWGG 2 cut(s) 822, 1186
PspN4I GGNNCC 3 cut(s) 556, 942, 1137
PspPI GGNCC 2 cut(s) 941, 1196
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 1 cut(s) 1135
PvuII CAGCTG 2 cut(s) 398, 980
RsaI GTAC 4 cut(s) 74, 342, 720, 793
RsaNI GTAC 4 cut(s) 73, 341, 719, 792
RseI CAYNNNNRTG 3 cut(s) 62, 125, 597
SaqAI TTAA 3 cut(s) 254, 434, 734
SatI GCNGC 6 cut(s) 375, 396, 502, 505, 652, 981
Sau3AI GATC 5 cut(s) 66, 136, 193, 451, 1135
Sau96I GGNCC 2 cut(s) 941, 1196
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 824, 1188
SduI GDGCHC 1 cut(s) 1094
SfaNI GCATC 4 cut(s) 129, 925, 1018, 1066
SfcI CTRYAG 1 cut(s) 657
SinI GGWCC 1 cut(s) 1196
SmiMI CAYNNNNRTG 3 cut(s) 62, 125, 597
Sse9I AATT 6 cut(s) 261, 361, 782, 1021, 1065, 1148
SsiI CCGC 4 cut(s) 16, 502, 938, 950
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 2 cut(s) 822, 1186
TaaI ACNGT 5 cut(s) 166, 563, 676, 800, 1043
TaiI ACGT 1 cut(s) 967
TaqI TCGA 2 cut(s) 271, 1153
TasI AATT 6 cut(s) 261, 361, 782, 1021, 1065, 1148
TatI WGTACW 3 cut(s) 72, 340, 791
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 240, 303
Tru1I TTAA 3 cut(s) 254, 434, 734
Tru9I TTAA 3 cut(s) 254, 434, 734
TscAI CASTG 2 cut(s) 169, 599
TseFI GTSAC 2 cut(s) 34, 127
TseI GCWGC 5 cut(s) 374, 395, 504, 651, 980
Tsp45I GTSAC 2 cut(s) 34, 127
TspDTI ATGAA 5 cut(s) 342, 452, 800, 866, 1230
TspGWI ACGGA 2 cut(s) 160, 1128
TspRI CASTG 2 cut(s) 169, 599
VpaK11BI GGWCC 1 cut(s) 1196
XagI CCTNNNNNAGG 1 cut(s) 1191
XapI RAATTY 3 cut(s) 261, 1021, 1148
XceI RCATGY 2 cut(s) 341, 834
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 1 cut(s) 413
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.