Rorug01G0100700
ERF Family

Performs the first committed step in the biosynthesis of isoprene-containing compounds such as sterols and terpenoids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000873
Physical Location & Seq
Reverse (-)
16772300 .. 16774808
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0100700.1

Sequence Viewer

Length: 2178 bp
ATGTCTAGGACCATAAAGAAAATATTTTTGCTGCGATTGGTGCTGGTTGCAATAGAGGTGCTACTAGTATCATCCCAAACGGTGGATATTACAAGAGAGGGTTGCCAAGACAAATGTGGCAATGTTAGCATTCCCTATCCGTTTGGAACAAGAGAGGGTTGTTACTATGATGAGGATTTCCTCATTACTTGTAATAGCACAAGTTATAACCCTCCGAAGTCATTTCTTCGAGTGAGTGACATTGAGGTCACAAACATATCCATTGATGGGAAACTCTATATTACTCAAGACATAGCCAGGGCTTGTTTTACCGAATCAGGTGCGCCCTATTATGGCAACTACAACCCTTATCTCTCTTTGAAAAAGTTCTACATCTCAGACACCGACAACAAATTCACAGGTGTTGGTTGTGACACCTATGCCGAAATTCAGGGCTACCAGGGTTGTTGCCAAACACCTATTGCTAAAGGAATGACTTACTTTACCGTAAGCGTAAGCAGCTACAATAATCAAACGGAAGTGTGGAATTTCAATCCCTGCAGCTATGCTTTTCTTGTCCAAGAAGGTAAGTTCAACTTCACCTCCGACATGTTGAGCGATTTAAGAAATGTCTCTCAGTTTCCAGTGGTTCTCGATTGGTCGATTGGAAATGAGACATGCAACCAGATCAAGAACAATGAATGTAAGGGGAACAGCAAGTGTTTGGATGTCAAGAATGGGCTCGGGTATCGCTGCAAGTGCAACGAAGGTTACGATGGGAATCCATACCTAAATGGATGCACTGACATTAATGAATGCGAGGATCCGGTGATTGCCAATAAGTGCAAACAAAGATGCATAAATACAGAGGGGAACTATACGTGTGCTTGCCACAAGGGGTACCATAACAATGGCACAGCAGACTCGGTAGAATGCATCGCTGACCACCAAACAATATTTGTTCAAATTATACTTGGGCTCGGTGTTGGCCTCATATCCTTATTAATAGGCATCTCTTGGTTGTACTTGGTCTACAAAAGATGGAAGCTCATGAAGCTCAAGGAGAAGTTCTTCATCCAAAATGGAGGATTGATGTTAAAGCAACAGCTTTCGGAAAGACAAGGTGGTGCCACTAACCAAACGGCCAAAATCTTCACCGAAGACCAACTTAAGAAGGCTACCAACCATTTCAGTGAAGCCAGAATCGTTGGCAAAGGAGGTTTCGGTACAGTCTACAAAGGGATTATAGCAGATGAAAGAGCTAAGGAAACAGTGGTTGCAATCAAGAAATCCAAACTTGTGGATCGAAGCCAAATAGAGCAATTCATTAATGAAGTAGTGGTGCTTTCCCAAATTAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCACTACTGGTTTATGAGTTTGTCACCAATGGTACGCTATTCGACTACATCCACAACAAAAGCAAGGAATCCAACTTTGCTTGGGAAATTCGTTTAAGGATAGCTGCAGAAACTGCTGGAGTGCTATCACACTTGCACTCTGAAGCTTCTATTCCCGTCATTCACAGAGATGTCAAGTCCACAAACATACTCTTGGATGATAATCTTACAGCAAAAGTGTCTGATTTCGGTGCCTCCAGATTGGTTCCGTCAGATCAAGCTCAGCTCTCTACAGTGGTGCAAGGAACGGTAGGGTATTTGGACCCTGAATACTTACAGACGAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTTCTTGTGGAACTACTAACAGGAAAGAAGGCGCTTTCGGTCGACAAGCCGGAAAAGGAGAGAAATCTAGCCATGTATTTTCTTTCTGCCCTGAGAGAGGACCGATTGGTAGAGGTGATTGATGGTAGTGTCATTAATGGAGCAAACGTTGAGCAGCTTAAGGAAGTATCAAACCTTGCAAAGAGGTGTTTGAAAGTAGAAGGAAAAGAAAGACCAACCATGAAGGAAGTGGCAATGGAGCTTGAGGGACTGAGAAGGATGGTAATGCATCCATGGGTTAGTAATGATGAATCCAATGCAGAGGAGAATGAACACTTGCTTGGTGAAATATCACTGGAAACTTTTAGTCATGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACCATGAGGAACCCGATCATATTACCAGTTGATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004163 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006066 GO:0006084 GO:0006139 GO:0006163 GO:0006464 GO:0006486 GO:0006487 GO:0006488 GO:0006489 GO:0006490 GO:0006629 GO:0006637 GO:0006644 GO:0006694 GO:0006695 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008202 GO:0008203 GO:0008284 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009889 GO:0009987 GO:0016125 GO:0016126 GO:0016829 GO:0016830 GO:0016831 GO:0019216 GO:0019218 GO:0019222 GO:0019287 GO:0019538 GO:0019637 GO:0019693 GO:0030544 GO:0031072 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0034645 GO:0035383 GO:0036211 GO:0042127 GO:0042579 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045540 GO:0046165 GO:0046465 GO:0046483 GO:0046490 GO:0046890 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050810 GO:0051186 GO:0055086 GO:0062012 GO:0065007 GO:0070085 GO:0071704 GO:0072521 GO:0080090 GO:0090181 GO:0090407 GO:0106118 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902652 GO:1902653 GO:1902930
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
GHMP_kinases_N

Protein Analysis

725

Amino Acids

80.96

Weight (kDa)

5.59

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 90 3.4e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 262 - 299 1.8e-06 Calcium-binding EGF domain
Pkinase PF00069 391 - 660 2.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 394 - 664 1.1e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AasI GACNNNNNNGTC 1 cut(s) 245
Acc65I GGTACC 1 cut(s) 879
AccB1I GGYRCC 3 cut(s) 879, 1106, 1616
AccB7I CCANNNNNTGG 1 cut(s) 82
AccI GTMKAC 3 cut(s) 1011, 1212, 1791
AclI AACGTT 1 cut(s) 1896
AclWI GGATC 3 cut(s) 797, 810, 1290
AcoI YGGCCR 1 cut(s) 1122
AcsI RAATTY 4 cut(s) 392, 426, 526, 1473
AcuI CTGAAG 1 cut(s) 1548
AfaI GTAC 4 cut(s) 881, 1004, 1207, 1420
AfiI CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1393, 1846
AflII CTTAAG 2 cut(s) 1148, 1907
AflIII ACRYGT 2 cut(s) 588, 860
AgsI TTSAA 5 cut(s) 361, 532, 574, 944, 1942
AhlI ACTAGT 1 cut(s) 64
AjnI CCWGG 2 cut(s) 296, 438
AjuI GAANNNNNNNTTGG 2 cut(s) 2052, 2084
Alw26I GTCTC 3 cut(s) 616, 647, 1370
AlwI GGATC 3 cut(s) 797, 810, 1290
AlwNI CAGNNNCTG 1 cut(s) 1499
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 2 cut(s) 967, 1122
ApeKI GCWGC 6 cut(s) 31, 498, 540, 732, 1490, 1903
ApoI RAATTY 4 cut(s) 392, 426, 526, 1473
AseI ATTAAT 5 cut(s) 789, 983, 1308, 1335, 1884
Asp700I GAANNNNTTC 2 cut(s) 365, 1049
Asp718I GGTACC 1 cut(s) 879
AspLEI GCGC 2 cut(s) 325, 1783
AspS9I GGNCC 3 cut(s) 9, 1687, 1849
AsuHPI GGTGA 7 cut(s) 571, 820, 1126, 1363, 1402, 1876, 2084
AvaI CYCGRG 1 cut(s) 722
AvaII GGWCC 3 cut(s) 9, 1687, 1849
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 3 cut(s) 879, 1106, 1616
BanII GRGCYC 2 cut(s) 723, 960
BarI GAAGNNNNNNTAC 2 cut(s) 1374, 1406
BbsI GAAGAC 1 cut(s) 1146
BbvI GCAGC 6 cut(s) 18, 510, 552, 719, 1477, 1915
BccI CCATC 6 cut(s) 260, 749, 1014, 1865, 2002, 2162
BceAI ACGGC 1 cut(s) 1137
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 2 cut(s) 298, 440
BcoDI GTCTC 3 cut(s) 616, 647, 1370
BcuI ACTAGT 1 cut(s) 64
BfaI CTAG 3 cut(s) 6, 65, 1817
BfmI CTRYAG 4 cut(s) 538, 1491, 1656, 1735
BfoI RGCGCY 1 cut(s) 1784
BfrI CTTAAG 2 cut(s) 1148, 1907
BisI GCNGC 6 cut(s) 32, 499, 541, 733, 1491, 1904
BlpI GCTNAGC 1 cut(s) 1647
BlsI GCNGC 6 cut(s) 33, 500, 542, 734, 1492, 1905
Bme1390I CCNGG 2 cut(s) 298, 440
Bme18I GGWCC 3 cut(s) 9, 1687, 1849
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 3 cut(s) 9, 1687, 1849
BmiI GGNNCC 7 cut(s) 804, 881, 1108, 1618, 1632, 1689, 2144
BmrFI CCNGG 2 cut(s) 298, 440
BmsI GCATC 5 cut(s) 767, 824, 924, 999, 2026
BoxI GACNNNNGTC 1 cut(s) 1382
BpiI GAAGAC 1 cut(s) 1146
BpmI CTGGAG 2 cut(s) 1524, 1606
Bpu10I CCTNAGC 1 cut(s) 1242
Bpu1102I GCTNAGC 1 cut(s) 1647
BpuEI CTTGAG 3 cut(s) 270, 1022, 2012
BsaAI YACGTR 1 cut(s) 861
BsaBI GATNNNNATC 2 cut(s) 759, 1587
BsaJI CCNNGG 3 cut(s) 297, 439, 2021
BsaWI WCCGGW 1 cut(s) 805
BsaXI ACNNNNNCTCC 4 cut(s) 566, 596, 1881, 1911
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1393, 1846
Bse1I ACTGG 5 cut(s) 623, 1398, 1711, 2088, 2159
Bse3DI GCAATG 2 cut(s) 127, 1989
Bse8I GATNNNNATC 2 cut(s) 759, 1587
BseBI CCWGG 2 cut(s) 298, 440
BseDI CCNNGG 3 cut(s) 297, 439, 2021
BseGI GGATG 8 cut(s) 71, 712, 782, 1053, 1434, 1588, 2013, 2017
BseJI GATNNNNATC 2 cut(s) 759, 1587
BseLI CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1393, 1846
BseMI GCAATG 2 cut(s) 127, 1989
BseMII CTCAG 5 cut(s) 390, 629, 1661, 1832, 1991
BseNI ACTGG 5 cut(s) 623, 1398, 1711, 2088, 2159
BseRI GAGGAG 1 cut(s) 2066
BseXI GCAGC 6 cut(s) 18, 510, 552, 719, 1477, 1915
Bsh1285I CGRYCG 1 cut(s) 1791
BshFI GGCC 2 cut(s) 969, 1124
BshNI GGYRCC 3 cut(s) 879, 1106, 1616
BsiEI CGRYCG 1 cut(s) 1791
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 2 cut(s) 806, 1799
BslFI GGGAC 2 cut(s) 1370, 2010
BslI CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1393, 1846
BsmAI GTCTC 3 cut(s) 616, 647, 1370
BsmFI GGGAC 2 cut(s) 1370, 2010
BsmI GAATGC 3 cut(s) 129, 800, 917
BsnI GGCC 2 cut(s) 969, 1124
BsoBI CYCGRG 1 cut(s) 722
Bsp1286I GDGCHC 2 cut(s) 723, 960
Bsp143I GATC 5 cut(s) 666, 802, 1282, 1639, 2148
Bsp1720I GCTNAGC 1 cut(s) 1647
Bsp19I CCATGG 1 cut(s) 2021
BspANI GGCC 2 cut(s) 969, 1124
BspCNI CTCAG 5 cut(s) 389, 628, 1660, 1833, 1992
BspHI TCATGA 1 cut(s) 1029
BspLI GGNNCC 7 cut(s) 804, 881, 1108, 1618, 1632, 1689, 2144
BspMAI CTGCAG 2 cut(s) 542, 1495
BspPI GGATC 3 cut(s) 797, 810, 1290
BspT107I GGYRCC 3 cut(s) 879, 1106, 1616
BspTI CTTAAG 2 cut(s) 1148, 1907
BsrDI GCAATG 2 cut(s) 127, 1989
BsrI ACTGG 5 cut(s) 623, 1398, 1711, 2088, 2159
BssECI CCNNGG 3 cut(s) 297, 439, 2021
BssMI GATC 5 cut(s) 666, 802, 1282, 1639, 2148
BssT1I CCWWGG 1 cut(s) 2021
Bst2UI CCWGG 2 cut(s) 298, 440
Bst4CI ACNGT 6 cut(s) 82, 487, 1210, 1252, 1660, 1675
BstAFI CTTAAG 2 cut(s) 1148, 1907
BstAPI GCANNNNNTGC 1 cut(s) 1499
BstBAI YACGTR 1 cut(s) 861
BstC8I GCNNGC 1 cut(s) 868
BstDEI CTNAG 6 cut(s) 376, 615, 1242, 1647, 1841, 2000
BstDSI CCRYGG 1 cut(s) 2021
BstENI CCTNNNNNAGG 1 cut(s) 1844
BstF5I GGATG 8 cut(s) 71, 712, 782, 1053, 1434, 1588, 2013, 2017
BstH2I RGCGCY 1 cut(s) 1784
BstHHI GCGC 2 cut(s) 325, 1783
BstKTI GATC 5 cut(s) 669, 805, 1285, 1642, 2151
BstMAI GTCTC 3 cut(s) 616, 647, 1370
BstMBI GATC 5 cut(s) 666, 802, 1282, 1639, 2148
BstMCI CGRYCG 1 cut(s) 1791
BstMWI GCNNNNNNNGC 6 cut(s) 40, 126, 498, 738, 1033, 1499
BstNI CCWGG 2 cut(s) 298, 440
BstNSI RCATGY 2 cut(s) 592, 660
BstPAI GACNNNNGTC 1 cut(s) 1382
BstSCI CCNGG 2 cut(s) 296, 438
BstSFI CTRYAG 4 cut(s) 538, 1491, 1656, 1735
BstV1I GCAGC 6 cut(s) 18, 510, 552, 719, 1477, 1915
BstV2I GAAGAC 1 cut(s) 1146
BstX2I RGATCY 1 cut(s) 802
BstXI CCANNNNNNTGG 2 cut(s) 890, 1279
BstYI RGATCY 1 cut(s) 802
BsuRI GGCC 2 cut(s) 969, 1124
BtgI CCRYGG 1 cut(s) 2021
BtgZI GCGATG 1 cut(s) 901
BtsCI GGATG 8 cut(s) 71, 712, 782, 1053, 1434, 1588, 2013, 2017
BtsIMutI CAGTG 6 cut(s) 630, 780, 1177, 1257, 1665, 2081
Cac8I GCNNGC 1 cut(s) 868
CaiI CAGNNNCTG 1 cut(s) 1499
CciI TCATGA 1 cut(s) 1029
CfoI GCGC 2 cut(s) 325, 1783
Cfr13I GGNCC 3 cut(s) 9, 1687, 1849
Csp6I GTAC 4 cut(s) 880, 1003, 1206, 1419
CviAII CATG 8 cut(s) 589, 657, 1030, 1822, 1969, 2022, 2099, 2137
CviQI GTAC 4 cut(s) 880, 1003, 1206, 1419
DdeI CTNAG 6 cut(s) 376, 615, 1242, 1647, 1841, 2000
DpnI GATC 5 cut(s) 668, 804, 1284, 1641, 2150
DpnII GATC 5 cut(s) 666, 802, 1282, 1639, 2148
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 1122
Eco130I CCWWGG 1 cut(s) 2021
Eco24I GRGCYC 2 cut(s) 723, 960
Eco47I GGWCC 3 cut(s) 9, 1687, 1849
Eco57I CTGAAG 1 cut(s) 1548
Eco88I CYCGRG 1 cut(s) 722
EcoNI CCTNNNNNAGG 1 cut(s) 1844
EcoRII CCWGG 2 cut(s) 296, 438
EcoT14I CCWWGG 1 cut(s) 2021
EcoT22I ATGCAT 3 cut(s) 839, 917, 2019
EcoT38I GRGCYC 2 cut(s) 723, 960
ErhI CCWWGG 1 cut(s) 2021
FaeI CATG 8 cut(s) 592, 660, 1033, 1825, 1972, 2025, 2102, 2140
FalI AAGNNNNNCTT 4 cut(s) 560, 592, 1131, 1163
FaqI GGGAC 2 cut(s) 1370, 2010
FatI CATG 8 cut(s) 588, 656, 1029, 1821, 1968, 2021, 2098, 2136
FblI GTMKAC 3 cut(s) 1011, 1212, 1791
Fnu4HI GCNGC 6 cut(s) 32, 499, 541, 733, 1491, 1904
FokI GGATG 8 cut(s) 58, 719, 789, 1040, 1421, 1595, 2004, 2020
FriOI GRGCYC 2 cut(s) 723, 960
Fsp4HI GCNGC 6 cut(s) 32, 499, 541, 733, 1491, 1904
FspBI CTAG 3 cut(s) 6, 65, 1817
GlaI GCGC 2 cut(s) 324, 1782
GluI GCNGC 6 cut(s) 32, 499, 541, 733, 1491, 1904
GsuI CTGGAG 2 cut(s) 1524, 1606
HaeII RGCGCY 1 cut(s) 1784
HaeIII GGCC 2 cut(s) 969, 1124
HapII CCGG 2 cut(s) 806, 1799
HhaI GCGC 2 cut(s) 325, 1783
Hin1II CATG 8 cut(s) 592, 660, 1033, 1825, 1972, 2025, 2102, 2140
Hin6I GCGC 2 cut(s) 323, 1781
HinP1I GCGC 2 cut(s) 323, 1781
HincII GTYRAC 2 cut(s) 1716, 1792
HindII GTYRAC 2 cut(s) 1716, 1792
HindIII AAGCTT 2 cut(s) 1355, 1530
HinfI GANTC 6 cut(s) 314, 760, 902, 1182, 1454, 2039
HpaII CCGG 2 cut(s) 806, 1799
HphI GGTGA 7 cut(s) 571, 820, 1126, 1363, 1402, 1876, 2084
Hpy166II GTNNAC 5 cut(s) 1012, 1213, 1566, 1716, 1792
Hpy188I TCNGA 7 cut(s) 216, 379, 586, 1093, 1528, 1609, 1639
Hpy188III TCNNGA 8 cut(s) 287, 632, 670, 712, 1030, 1264, 1375, 1623
Hpy8I GTNNAC 5 cut(s) 1012, 1213, 1566, 1716, 1792
HpyAV CCTTC 7 cut(s) 557, 740, 1147, 1771, 1943, 1966, 1998
HpyCH4III ACNGT 6 cut(s) 82, 487, 1210, 1252, 1660, 1675
HpyCH4IV ACGT 2 cut(s) 860, 1896
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 126, 498, 738, 1033, 1499
HpyF3I CTNAG 6 cut(s) 376, 615, 1242, 1647, 1841, 2000
HpySE526I ACGT 2 cut(s) 860, 1896
Hsp92II CATG 8 cut(s) 592, 660, 1033, 1825, 1972, 2025, 2102, 2140
HspAI GCGC 2 cut(s) 323, 1781
KpnI GGTACC 1 cut(s) 883
Kzo9I GATC 5 cut(s) 666, 802, 1282, 1639, 2148
LmnI GCTCC 2 cut(s) 1889, 1987
Lsp1109I GCAGC 6 cut(s) 18, 510, 552, 719, 1477, 1915
LweI GCATC 5 cut(s) 767, 824, 924, 999, 2026
MaeI CTAG 3 cut(s) 6, 65, 1817
MaeII ACGT 2 cut(s) 860, 1896
MaeIII GTNAC 6 cut(s) 161, 236, 247, 410, 749, 1408
MalI GATC 5 cut(s) 668, 804, 1284, 1641, 2150
MboI GATC 5 cut(s) 666, 802, 1282, 1639, 2148
MboII GAAGA 4 cut(s) 218, 1042, 1123, 1151
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 2 cut(s) 723, 960
MluCI AATT 7 cut(s) 392, 426, 526, 945, 1301, 1332, 1473
MlyI GAGTC 1 cut(s) 896
MmeI TCCRAC 2 cut(s) 609, 1482
Mph1103I ATGCAT 3 cut(s) 839, 917, 2019
MroXI GAANNNNTTC 2 cut(s) 365, 1049
MslI CAYNNNNRTG 5 cut(s) 888, 1170, 1344, 1554, 2103
MspCI CTTAAG 2 cut(s) 1148, 1907
MspI CCGG 2 cut(s) 806, 1799
MspR9I CCNGG 2 cut(s) 298, 440
Mva1269I GAATGC 3 cut(s) 129, 800, 917
MvaI CCWGG 2 cut(s) 298, 440
MwoI GCNNNNNNNGC 6 cut(s) 40, 126, 498, 738, 1033, 1499
NcoI CCATGG 1 cut(s) 2021
NdeII GATC 5 cut(s) 666, 802, 1282, 1639, 2148
NlaIII CATG 8 cut(s) 592, 660, 1033, 1825, 1972, 2025, 2102, 2140
NlaIV GGNNCC 7 cut(s) 804, 881, 1108, 1618, 1632, 1689, 2144
NmuCI GTSAC 4 cut(s) 236, 247, 410, 1408
NsiI ATGCAT 3 cut(s) 839, 917, 2019
NspI RCATGY 2 cut(s) 592, 660
PagI TCATGA 1 cut(s) 1029
PciI ACATGT 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 750
PctI GAATGC 3 cut(s) 129, 800, 917
PdmI GAANNNNTTC 2 cut(s) 365, 1049
PfeI GAWTC 5 cut(s) 314, 760, 1182, 1454, 2039
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 6 cut(s) 33, 500, 542, 734, 1492, 1905
PleI GAGTC 1 cut(s) 896
PpsI GAGTC 1 cut(s) 896
Ppu21I YACGTR 1 cut(s) 861
PscI ACATGT 1 cut(s) 588
PshAI GACNNNNGTC 1 cut(s) 1382
PshBI ATTAAT 5 cut(s) 789, 983, 1308, 1335, 1884
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 1896
Psp6I CCWGG 2 cut(s) 296, 438
PspGI CCWGG 2 cut(s) 296, 438
PspN4I GGNNCC 7 cut(s) 804, 881, 1108, 1618, 1632, 1689, 2144
PspPI GGNCC 3 cut(s) 9, 1687, 1849
PstI CTGCAG 2 cut(s) 542, 1495
PstNI CAGNNNCTG 1 cut(s) 1499
PsuI RGATCY 1 cut(s) 802
RsaI GTAC 4 cut(s) 881, 1004, 1207, 1420
RsaNI GTAC 4 cut(s) 880, 1003, 1206, 1419
RseI CAYNNNNRTG 5 cut(s) 888, 1170, 1344, 1554, 2103
SalI GTCGAC 1 cut(s) 1790
SatI GCNGC 6 cut(s) 32, 499, 541, 733, 1491, 1904
Sau3AI GATC 5 cut(s) 666, 802, 1282, 1639, 2148
Sau96I GGNCC 3 cut(s) 9, 1687, 1849
SchI GAGTC 1 cut(s) 896
ScrFI CCNGG 2 cut(s) 298, 440
SduI GDGCHC 2 cut(s) 723, 960
SfaNI GCATC 5 cut(s) 767, 824, 924, 999, 2026
SfcI CTRYAG 4 cut(s) 538, 1491, 1656, 1735
SinI GGWCC 3 cut(s) 9, 1687, 1849
SmiMI CAYNNNNRTG 5 cut(s) 888, 1170, 1344, 1554, 2103
SmlI CTYRAG 5 cut(s) 285, 1037, 1148, 1907, 1991
SmoI CTYRAG 5 cut(s) 285, 1037, 1148, 1907, 1991
SpeI ACTAGT 1 cut(s) 64
Sse9I AATT 7 cut(s) 392, 426, 526, 945, 1301, 1332, 1473
SspI AATATT 2 cut(s) 24, 936
SspMI CTAG 3 cut(s) 6, 65, 1817
StyD4I CCNGG 2 cut(s) 296, 438
StyI CCWWGG 1 cut(s) 2021
TaaI ACNGT 6 cut(s) 82, 487, 1210, 1252, 1660, 1675
TaiI ACGT 2 cut(s) 863, 1899
TaqI TCGA 7 cut(s) 229, 633, 641, 1285, 1374, 1428, 1791
TaqII GACCGA 2 cut(s) 1777, 1866
TasI AATT 7 cut(s) 392, 426, 526, 945, 1301, 1332, 1473
TatI WGTACW 1 cut(s) 1002
TfiI GAWTC 5 cut(s) 314, 760, 1182, 1454, 2039
TscAI CASTG 6 cut(s) 630, 787, 1177, 1257, 1665, 2088
TseFI GTSAC 4 cut(s) 236, 247, 410, 1408
TseI GCWGC 6 cut(s) 31, 498, 540, 732, 1490, 1903
Tsp45I GTSAC 4 cut(s) 236, 247, 410, 1408
TspGWI ACGGA 3 cut(s) 129, 530, 1623
TspRI CASTG 6 cut(s) 630, 787, 1177, 1257, 1665, 2088
Van91I CCANNNNNTGG 1 cut(s) 82
Vha464I CTTAAG 2 cut(s) 1148, 1907
VpaK11BI GGWCC 3 cut(s) 9, 1687, 1849
VspI ATTAAT 5 cut(s) 789, 983, 1308, 1335, 1884
XagI CCTNNNNNAGG 1 cut(s) 1844
XapI RAATTY 4 cut(s) 392, 426, 526, 1473
XceI RCATGY 2 cut(s) 592, 660
XcmI CCANNNNNNNNNTGG 2 cut(s) 113, 1975
XmiI GTMKAC 3 cut(s) 1011, 1212, 1791
XmnI GAANNNNTTC 2 cut(s) 365, 1049
XspI CTAG 3 cut(s) 6, 65, 1817
Zsp2I ATGCAT 3 cut(s) 839, 917, 2019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.