Alpha/beta hydrolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 389 |
| AccI | GTMKAC | 2 cut(s) | 617, 843 |
| AciI | CCGC | 5 cut(s) | 66, 143, 162, 174, 821 |
| AclWI | GGATC | 5 cut(s) | 395, 401, 468, 481, 657 |
| AcoI | YGGCCR | 2 cut(s) | 258, 593 |
| AcsI | RAATTY | 1 cut(s) | 479 |
| AcuI | CTGAAG | 1 cut(s) | 515 |
| AdeI | CACNNNGTG | 1 cut(s) | 367 |
| AfaI | GTAC | 3 cut(s) | 360, 536, 807 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 389, 699 |
| AgsI | TTSAA | 2 cut(s) | 983, 989 |
| AjnI | CCWGG | 3 cut(s) | 169, 382, 595 |
| AlwI | GGATC | 5 cut(s) | 395, 401, 468, 481, 657 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1043 |
| Ama87I | CYCGRG | 1 cut(s) | 644 |
| AoxI | GGCC | 6 cut(s) | 132, 175, 225, 258, 593, 818 |
| ApeKI | GCWGC | 2 cut(s) | 49, 832 |
| ApoI | RAATTY | 1 cut(s) | 479 |
| AspS9I | GGNCC | 2 cut(s) | 176, 936 |
| AsuHPI | GGTGA | 4 cut(s) | 324, 404, 665, 968 |
| AsuNHI | GCTAGC | 1 cut(s) | 550 |
| AvaI | CYCGRG | 1 cut(s) | 644 |
| AvaII | GGWCC | 1 cut(s) | 936 |
| BaeGI | GKGCMC | 2 cut(s) | 661, 1050 |
| BalI | TGGCCA | 2 cut(s) | 260, 595 |
| BamHI | GGATCC | 1 cut(s) | 473 |
| BbsI | GAAGAC | 1 cut(s) | 521 |
| BbvI | GCAGC | 2 cut(s) | 61, 844 |
| BccI | CCATC | 6 cut(s) | 191, 206, 864, 879, 951, 1061 |
| BciT130I | CCWGG | 3 cut(s) | 171, 384, 597 |
| BfaI | CTAG | 3 cut(s) | 195, 551, 1104 |
| BfmI | CTRYAG | 2 cut(s) | 47, 900 |
| BisI | GCNGC | 4 cut(s) | 50, 175, 821, 833 |
| BlsI | GCNGC | 4 cut(s) | 51, 176, 822, 834 |
| Bme1390I | CCNGG | 3 cut(s) | 171, 384, 597 |
| Bme18I | GGWCC | 1 cut(s) | 936 |
| BmeT110I | CYCGRG | 1 cut(s) | 644 |
| BmgT120I | GGNCC | 2 cut(s) | 176, 936 |
| BmiI | GGNNCC | 3 cut(s) | 166, 475, 937 |
| BmrFI | CCNGG | 3 cut(s) | 171, 384, 597 |
| BmrI | ACTGGG | 1 cut(s) | 971 |
| BmsI | GCATC | 3 cut(s) | 298, 613, 780 |
| BmtI | GCTAGC | 1 cut(s) | 554 |
| BmuI | ACTGGG | 1 cut(s) | 971 |
| BpiI | GAAGAC | 1 cut(s) | 521 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 549, 581, 743, 775 |
| BpmI | CTGGAG | 3 cut(s) | 541, 576, 745 |
| Bpu10I | CCTNAGC | 1 cut(s) | 411 |
| BsaBI | GATNNNNATC | 2 cut(s) | 405, 472 |
| BsaJI | CCNNGG | 4 cut(s) | 169, 643, 683, 1013 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 389, 699 |
| Bse1I | ACTGG | 4 cut(s) | 593, 701, 762, 977 |
| Bse8I | GATNNNNATC | 2 cut(s) | 405, 472 |
| BseBI | CCWGG | 3 cut(s) | 171, 384, 597 |
| BseDI | CCNNGG | 4 cut(s) | 169, 643, 683, 1013 |
| BseGI | GGATG | 2 cut(s) | 81, 537 |
| BseJI | GATNNNNATC | 2 cut(s) | 405, 472 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 389, 699 |
| BseMII | CTCAG | 1 cut(s) | 722 |
| BseNI | ACTGG | 4 cut(s) | 593, 701, 762, 977 |
| BseSI | GKGCMC | 2 cut(s) | 661, 1050 |
| BseXI | GCAGC | 2 cut(s) | 61, 844 |
| BseYI | CCCAGC | 1 cut(s) | 1043 |
| BshFI | GGCC | 6 cut(s) | 134, 177, 227, 260, 595, 820 |
| BsiHKCI | CYCGRG | 1 cut(s) | 644 |
| BslFI | GGGAC | 1 cut(s) | 959 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 389, 699 |
| BsmFI | GGGAC | 1 cut(s) | 959 |
| BsnI | GGCC | 6 cut(s) | 134, 177, 227, 260, 595, 820 |
| BsoBI | CYCGRG | 1 cut(s) | 644 |
| Bsp1286I | GDGCHC | 2 cut(s) | 661, 1050 |
| Bsp143I | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| Bsp19I | CCATGG | 2 cut(s) | 683, 1013 |
| BspACI | CCGC | 5 cut(s) | 66, 143, 162, 174, 821 |
| BspANI | GGCC | 6 cut(s) | 134, 177, 227, 260, 595, 820 |
| BspCNI | CTCAG | 1 cut(s) | 721 |
| BspLI | GGNNCC | 3 cut(s) | 166, 475, 937 |
| BspMAI | CTGCAG | 1 cut(s) | 51 |
| BspOI | GCTAGC | 1 cut(s) | 554 |
| BspPI | GGATC | 5 cut(s) | 395, 401, 468, 481, 657 |
| BspQI | GCTCTTC | 1 cut(s) | 441 |
| BsrI | ACTGG | 4 cut(s) | 593, 701, 762, 977 |
| BssECI | CCNNGG | 4 cut(s) | 169, 643, 683, 1013 |
| BssMI | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| BssNAI | GTATAC | 1 cut(s) | 844 |
| BssT1I | CCWWGG | 2 cut(s) | 683, 1013 |
| Bst1107I | GTATAC | 1 cut(s) | 844 |
| Bst2UI | CCWGG | 3 cut(s) | 171, 384, 597 |
| Bst4CI | ACNGT | 2 cut(s) | 421, 739 |
| Bst6I | CTCTTC | 3 cut(s) | 342, 419, 441 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 317 |
| BstC8I | GCNNGC | 4 cut(s) | 117, 453, 552, 795 |
| BstDEI | CTNAG | 4 cut(s) | 206, 411, 708, 782 |
| BstDSI | CCRYGG | 2 cut(s) | 683, 1013 |
| BstF5I | GGATG | 2 cut(s) | 81, 537 |
| BstKTI | GATC | 5 cut(s) | 6, 403, 409, 476, 652 |
| BstMBI | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 131, 317, 829 |
| BstNI | CCWGG | 3 cut(s) | 171, 384, 597 |
| BstSCI | CCNGG | 3 cut(s) | 169, 382, 595 |
| BstSFI | CTRYAG | 2 cut(s) | 47, 900 |
| BstSLI | GKGCMC | 2 cut(s) | 661, 1050 |
| BstV1I | GCAGC | 2 cut(s) | 61, 844 |
| BstV2I | GAAGAC | 1 cut(s) | 521 |
| BstX2I | RGATCY | 2 cut(s) | 406, 473 |
| BstYI | RGATCY | 2 cut(s) | 406, 473 |
| BstZ17I | GTATAC | 1 cut(s) | 844 |
| BsuRI | GGCC | 6 cut(s) | 134, 177, 227, 260, 595, 820 |
| BtgI | CCRYGG | 2 cut(s) | 683, 1013 |
| BtsCI | GGATG | 2 cut(s) | 81, 537 |
| BtsI | GCAGTG | 1 cut(s) | 318 |
| BtsIMutI | CAGTG | 2 cut(s) | 318, 426 |
| Cac8I | GCNNGC | 4 cut(s) | 117, 453, 552, 795 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1043 |
| Cfr13I | GGNCC | 2 cut(s) | 176, 936 |
| Csp6I | GTAC | 3 cut(s) | 359, 535, 806 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 237, 272 |
| CviAII | CATG | 9 cut(s) | 120, 239, 284, 305, 684, 865, 1014, 1028, 1084 |
| CviQI | GTAC | 3 cut(s) | 359, 535, 806 |
| DdeI | CTNAG | 4 cut(s) | 206, 411, 708, 782 |
| DpnI | GATC | 5 cut(s) | 5, 402, 408, 475, 651 |
| DpnII | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| DraIII | CACNNNGTG | 1 cut(s) | 367 |
| EaeI | YGGCCR | 2 cut(s) | 258, 593 |
| Eam1104I | CTCTTC | 3 cut(s) | 342, 419, 441 |
| EarI | CTCTTC | 3 cut(s) | 342, 419, 441 |
| EciI | GGCGGA | 1 cut(s) | 81 |
| Eco130I | CCWWGG | 2 cut(s) | 683, 1013 |
| Eco47I | GGWCC | 1 cut(s) | 936 |
| Eco57I | CTGAAG | 1 cut(s) | 515 |
| Eco88I | CYCGRG | 1 cut(s) | 644 |
| EcoRII | CCWGG | 3 cut(s) | 169, 382, 595 |
| EcoT14I | CCWWGG | 2 cut(s) | 683, 1013 |
| ErhI | CCWWGG | 2 cut(s) | 683, 1013 |
| FaeI | CATG | 9 cut(s) | 123, 242, 287, 308, 687, 868, 1017, 1031, 1087 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 260, 292, 954, 986 |
| FaqI | GGGAC | 1 cut(s) | 959 |
| FatI | CATG | 9 cut(s) | 119, 238, 283, 304, 683, 864, 1013, 1027, 1083 |
| FblI | GTMKAC | 2 cut(s) | 617, 843 |
| Fnu4HI | GCNGC | 4 cut(s) | 50, 175, 821, 833 |
| FokI | GGATG | 2 cut(s) | 88, 524 |
| Fsp4HI | GCNGC | 4 cut(s) | 50, 175, 821, 833 |
| FspBI | CTAG | 3 cut(s) | 195, 551, 1104 |
| GluI | GCNGC | 4 cut(s) | 50, 175, 821, 833 |
| GsaI | CCCAGC | 1 cut(s) | 1047 |
| GsuI | CTGGAG | 3 cut(s) | 541, 576, 745 |
| HaeIII | GGCC | 6 cut(s) | 134, 177, 227, 260, 595, 820 |
| Hin1II | CATG | 9 cut(s) | 123, 242, 287, 308, 687, 868, 1017, 1031, 1087 |
| HincII | GTYRAC | 3 cut(s) | 583, 618, 1035 |
| HindII | GTYRAC | 3 cut(s) | 583, 618, 1035 |
| HindIII | AAGCTT | 1 cut(s) | 1007 |
| HinfI | GANTC | 6 cut(s) | 27, 69, 87, 427, 468, 848 |
| HpaI | GTTAAC | 1 cut(s) | 1035 |
| HphI | GGTGA | 4 cut(s) | 324, 404, 665, 968 |
| Hpy166II | GTNNAC | 7 cut(s) | 361, 509, 583, 618, 746, 844, 1035 |
| Hpy188I | TCNGA | 4 cut(s) | 153, 467, 473, 711 |
| Hpy188III | TCNNGA | 9 cut(s) | 7, 34, 73, 300, 404, 520, 767, 860, 910 |
| Hpy8I | GTNNAC | 7 cut(s) | 361, 509, 583, 618, 746, 844, 1035 |
| HpyAV | CCTTC | 2 cut(s) | 732, 926 |
| HpyCH4III | ACNGT | 2 cut(s) | 421, 739 |
| HpyCH4V | TGCA | 4 cut(s) | 49, 311, 793, 1058 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 131, 317, 829 |
| HpyF3I | CTNAG | 4 cut(s) | 206, 411, 708, 782 |
| Hsp92II | CATG | 9 cut(s) | 123, 242, 287, 308, 687, 868, 1017, 1031, 1087 |
| KspAI | GTTAAC | 1 cut(s) | 1035 |
| Kzo9I | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| LguI | GCTCTTC | 1 cut(s) | 441 |
| LmnI | GCTCC | 5 cut(s) | 196, 235, 522, 557, 577 |
| Lsp1109I | GCAGC | 2 cut(s) | 61, 844 |
| LweI | GCATC | 3 cut(s) | 298, 613, 780 |
| MaeI | CTAG | 3 cut(s) | 195, 551, 1104 |
| MaeIII | GTNAC | 2 cut(s) | 733, 860 |
| MalI | GATC | 5 cut(s) | 5, 402, 408, 475, 651 |
| MboI | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| MboII | GAAGA | 9 cut(s) | 166, 228, 288, 359, 436, 458, 521, 799, 974 |
| MflI | RGATCY | 2 cut(s) | 406, 473 |
| MhlI | GDGCHC | 2 cut(s) | 661, 1050 |
| MlsI | TGGCCA | 2 cut(s) | 260, 595 |
| MluCI | AATT | 2 cut(s) | 479, 713 |
| MluNI | TGGCCA | 2 cut(s) | 260, 595 |
| MlyI | GAGTC | 4 cut(s) | 36, 96, 436, 857 |
| MmeI | TCCRAC | 2 cut(s) | 88, 472 |
| MnlI | CCTC | 3 cut(s) | 159, 552, 653 |
| Mox20I | TGGCCA | 2 cut(s) | 260, 595 |
| MscI | TGGCCA | 2 cut(s) | 260, 595 |
| MseI | TTAA | 2 cut(s) | 1034, 1088 |
| Msp20I | TGGCCA | 2 cut(s) | 260, 595 |
| MspR9I | CCNGG | 3 cut(s) | 171, 384, 597 |
| MvaI | CCWGG | 3 cut(s) | 171, 384, 597 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 131, 317, 829 |
| NcoI | CCATGG | 2 cut(s) | 683, 1013 |
| NdeII | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| NheI | GCTAGC | 1 cut(s) | 550 |
| NlaIII | CATG | 9 cut(s) | 123, 242, 287, 308, 687, 868, 1017, 1031, 1087 |
| NlaIV | GGNNCC | 3 cut(s) | 166, 475, 937 |
| NmuCI | GTSAC | 1 cut(s) | 860 |
| PciSI | GCTCTTC | 1 cut(s) | 441 |
| PfeI | GAWTC | 2 cut(s) | 69, 468 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 389 |
| PkrI | GCNGC | 4 cut(s) | 51, 176, 822, 834 |
| PleI | GAGTC | 4 cut(s) | 35, 95, 435, 856 |
| PpsI | GAGTC | 4 cut(s) | 35, 95, 435, 856 |
| Psp6I | CCWGG | 3 cut(s) | 169, 382, 595 |
| PspFI | CCCAGC | 1 cut(s) | 1043 |
| PspGI | CCWGG | 3 cut(s) | 169, 382, 595 |
| PspN4I | GGNNCC | 3 cut(s) | 166, 475, 937 |
| PspPI | GGNCC | 2 cut(s) | 176, 936 |
| PstI | CTGCAG | 1 cut(s) | 51 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1043 |
| PsuI | RGATCY | 2 cut(s) | 406, 473 |
| RsaI | GTAC | 3 cut(s) | 360, 536, 807 |
| RsaNI | GTAC | 3 cut(s) | 359, 535, 806 |
| SalI | GTCGAC | 1 cut(s) | 616 |
| SapI | GCTCTTC | 1 cut(s) | 441 |
| SaqAI | TTAA | 2 cut(s) | 1034, 1088 |
| SatI | GCNGC | 4 cut(s) | 50, 175, 821, 833 |
| Sau3AI | GATC | 5 cut(s) | 3, 400, 406, 473, 649 |
| Sau96I | GGNCC | 2 cut(s) | 176, 936 |
| SchI | GAGTC | 4 cut(s) | 36, 96, 436, 857 |
| ScrFI | CCNGG | 3 cut(s) | 171, 384, 597 |
| SduI | GDGCHC | 2 cut(s) | 661, 1050 |
| SfaNI | GCATC | 3 cut(s) | 298, 613, 780 |
| SfcI | CTRYAG | 2 cut(s) | 47, 900 |
| SinI | GGWCC | 1 cut(s) | 936 |
| Sse9I | AATT | 2 cut(s) | 479, 713 |
| SsiI | CCGC | 5 cut(s) | 66, 143, 162, 174, 821 |
| SspMI | CTAG | 3 cut(s) | 195, 551, 1104 |
| StyD4I | CCNGG | 3 cut(s) | 169, 382, 595 |
| StyI | CCWWGG | 2 cut(s) | 683, 1013 |
| TaaI | ACNGT | 2 cut(s) | 421, 739 |
| TaqI | TCGA | 5 cut(s) | 6, 345, 617, 664, 851 |
| TasI | AATT | 2 cut(s) | 479, 713 |
| TatI | WGTACW | 1 cut(s) | 534 |
| TauI | GCSGC | 2 cut(s) | 177, 823 |
| TfiI | GAWTC | 2 cut(s) | 69, 468 |
| Tru1I | TTAA | 2 cut(s) | 1034, 1088 |
| Tru9I | TTAA | 2 cut(s) | 1034, 1088 |
| TscAI | CASTG | 2 cut(s) | 318, 426 |
| TseFI | GTSAC | 1 cut(s) | 860 |
| TseI | GCWGC | 2 cut(s) | 49, 832 |
| Tsp45I | GTSAC | 1 cut(s) | 860 |
| TspDTI | ATGAA | 5 cut(s) | 272, 714, 945, 975, 1092 |
| TspGWI | ACGGA | 2 cut(s) | 840, 928 |
| TspRI | CASTG | 2 cut(s) | 318, 426 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 389 |
| VpaK11BI | GGWCC | 1 cut(s) | 936 |
| XapI | RAATTY | 1 cut(s) | 479 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 769 |
| XmiI | GTMKAC | 2 cut(s) | 617, 843 |
| XspI | CTAG | 3 cut(s) | 195, 551, 1104 |