Rh1BG095300

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000873
Physical Location & Seq
Reverse (-)
16626168 .. 16629515
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG095300.1

Sequence Viewer

Length: 1041 bp
ATGTTTAGGAGAGTCGCATTTTTGTTGTTGGTTGGATTGCTGGCATGGGCTTATCAGGCCGCCCAACCGCCCCCTCCAAAGGTTTGCGGTTCCCCCGGCAGTCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCCTACAAGGAGCATGGCGTGTCAAAACAAGTGGCCAGATATAAGATTATCTTTGTTCATGGGTTCGGTTCTTCCAGACATGATGCAGTGATTGCGACCAAACCTTCACCAGAACTTATTGAAGAGTTAGGGGTACACATTGTGTCTTTTGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGATCCAAATTTTATGTAGTTGGATATTCTATGGGTGGACAGGTTATCTGGAGTTGTCTTCAGTACATCCCTCACAGGCTAGCCGGAGCTACACTGTATGCTCCTGTTGTCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCAACAGCTGCCTACTATAAACAACCCCCTGGGGATCAATGGGCACTTCGAGTTGCTCACCATATACCATGGCTAACATACTGGTGGAACACTCAGAAATTATTTCCTTCATCAAGCGTTATAGTTCATAAACCAGAGATTTTCTCCAGTCAGGACTTGGAAATACTTAGGAAGATGCAGGCTTCTGGCATACAAACTAACAGACCACACGGAAAGCAGCAAGGAGTATACGAGTCGAACCATCGTGACATGATGATTGGATTTGGGAGTTGGGAGTTTGACCCTATGGAAATCAAGAACCCATTTCCCAGCAATGAAGGGTCCGTCCATCTGTGGCAGGGTGATGAAGATAGGCTTGTTCCAGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTAACTACCATGAGTTACCAGGTGCTGGGCACTTGTTTGCAAATAATGATGGTATAAATGAAGCTATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

38.98

Weight (kDa)

8.35

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 63 - 326 1.1e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 326 5.6e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 327 2.9e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 314, 968
AccI GTMKAC 1 cut(s) 768
AciI CCGC 3 cut(s) 60, 68, 87
AclWI GGATC 5 cut(s) 320, 326, 393, 406, 582
AcoI YGGCCR 2 cut(s) 183, 518
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 440
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 2 cut(s) 285, 461
AfiI CCNNNNNNNGG 3 cut(s) 79, 314, 968
AgsI TTSAA 3 cut(s) 272, 908, 914
AjnI CCWGG 4 cut(s) 307, 520, 568, 961
AluBI AGCT 8 cut(s) 116, 130, 376, 388, 485, 548, 934, 1007
AluI AGCT 8 cut(s) 116, 130, 376, 388, 485, 548, 934, 1007
AlwI GGATC 5 cut(s) 320, 326, 393, 406, 582
AlwNI CAGNNNCTG 1 cut(s) 968
AoxI GGCC 4 cut(s) 57, 150, 183, 518
ApeKI GCWGC 2 cut(s) 548, 757
ApoI RAATTY 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 861
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 4 cut(s) 249, 329, 590, 893
AsuNHI GCTAGC 1 cut(s) 475
AvaII GGWCC 1 cut(s) 861
BaeGI GKGCMC 2 cut(s) 586, 975
BalI TGGCCA 2 cut(s) 185, 520
BamHI GGATCC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 535, 769
BccI CCATC 5 cut(s) 116, 131, 789, 876, 986
BciT130I CCWGG 4 cut(s) 309, 522, 570, 963
BcnI CCSGG 1 cut(s) 96
BfaI CTAG 2 cut(s) 120, 476
BisI GCNGC 3 cut(s) 60, 549, 758
BlsI GCNGC 3 cut(s) 61, 550, 759
Bme1390I CCNGG 5 cut(s) 96, 309, 522, 570, 963
Bme18I GGWCC 1 cut(s) 861
BmgT120I GGNCC 1 cut(s) 861
BmiI GGNNCC 3 cut(s) 91, 400, 862
BmrFI CCNGG 5 cut(s) 96, 309, 522, 570, 963
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 2 cut(s) 223, 705
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 2 cut(s) 466, 670
Bpu10I CCTNAGC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 96
BsaBI GATNNNNATC 2 cut(s) 330, 397
BsaJI CCNNGG 5 cut(s) 94, 568, 569, 608, 938
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 314, 968
Bse1I ACTGG 5 cut(s) 518, 626, 687, 902, 1033
Bse3DI GCAATG 1 cut(s) 859
Bse8I GATNNNNATC 2 cut(s) 330, 397
BseBI CCWGG 4 cut(s) 309, 522, 570, 963
BseDI CCNNGG 5 cut(s) 94, 568, 569, 608, 938
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 2 cut(s) 330, 397
BseLI CCNNNNNNNGG 3 cut(s) 79, 314, 968
BseMI GCAATG 1 cut(s) 859
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BseSI GKGCMC 2 cut(s) 586, 975
BseXI GCAGC 2 cut(s) 535, 769
BseYI CCCAGC 2 cut(s) 848, 968
BshFI GGCC 4 cut(s) 59, 152, 185, 520
BsiSI CCGG 2 cut(s) 96, 480
BslI CCNNNNNNNGG 3 cut(s) 79, 314, 968
BsnI GGCC 4 cut(s) 59, 152, 185, 520
Bsp1286I GDGCHC 2 cut(s) 586, 975
Bsp143I GATC 4 cut(s) 325, 331, 398, 574
Bsp19I CCATGG 2 cut(s) 608, 938
BspACI CCGC 3 cut(s) 60, 68, 87
BspANI GGCC 4 cut(s) 59, 152, 185, 520
BspCNI CTCAG 1 cut(s) 646
BspLI GGNNCC 3 cut(s) 91, 400, 862
BspOI GCTAGC 1 cut(s) 479
BspPI GGATC 5 cut(s) 320, 326, 393, 406, 582
BspQI GCTCTTC 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 859
BsrI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BssECI CCNNGG 5 cut(s) 94, 568, 569, 608, 938
BssMI GATC 4 cut(s) 325, 331, 398, 574
BssNAI GTATAC 1 cut(s) 769
BssT1I CCWWGG 2 cut(s) 608, 938
Bst1107I GTATAC 1 cut(s) 769
Bst2UI CCWGG 4 cut(s) 309, 522, 570, 963
Bst4CI ACNGT 2 cut(s) 346, 492
Bst6I CTCTTC 3 cut(s) 267, 344, 366
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 4 cut(s) 42, 378, 477, 720
BstDEI CTNAG 4 cut(s) 131, 336, 633, 707
BstDSI CCRYGG 2 cut(s) 608, 938
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 4 cut(s) 328, 334, 401, 577
BstMBI GATC 4 cut(s) 325, 331, 398, 574
BstMWI GCNNNNNNNGC 2 cut(s) 56, 242
BstNI CCWGG 4 cut(s) 309, 522, 570, 963
BstSCI CCNGG 5 cut(s) 94, 307, 520, 568, 961
BstSLI GKGCMC 2 cut(s) 586, 975
BstV1I GCAGC 2 cut(s) 535, 769
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 2 cut(s) 331, 398
BstYI RGATCY 2 cut(s) 331, 398
BstZ17I GTATAC 1 cut(s) 769
BsuRI GGCC 4 cut(s) 59, 152, 185, 520
BtgI CCRYGG 2 cut(s) 608, 938
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 351, 488
Cac8I GCNNGC 4 cut(s) 42, 378, 477, 720
CaiI CAGNNNCTG 1 cut(s) 968
Cfr13I GGNCC 1 cut(s) 861
CsiI ACCWGGT 1 cut(s) 961
Csp6I GTAC 2 cut(s) 284, 460
CspCI CAANNNNNGTGG 2 cut(s) 162, 197
CviAII CATG 8 cut(s) 45, 164, 209, 230, 609, 790, 939, 953
CviQI GTAC 2 cut(s) 284, 460
DdeI CTNAG 4 cut(s) 131, 336, 633, 707
DpnI GATC 4 cut(s) 327, 333, 400, 576
DpnII GATC 4 cut(s) 325, 331, 398, 574
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 2 cut(s) 183, 518
Eam1104I CTCTTC 3 cut(s) 267, 344, 366
EarI CTCTTC 3 cut(s) 267, 344, 366
Eco130I CCWWGG 2 cut(s) 608, 938
Eco47I GGWCC 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 440
EcoRII CCWGG 4 cut(s) 307, 520, 568, 961
EcoT14I CCWWGG 2 cut(s) 608, 938
ErhI CCWWGG 2 cut(s) 608, 938
FaeI CATG 8 cut(s) 48, 167, 212, 233, 612, 793, 942, 956
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 879, 911
FatI CATG 8 cut(s) 44, 163, 208, 229, 608, 789, 938, 952
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 3 cut(s) 60, 549, 758
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 3 cut(s) 60, 549, 758
FspBI CTAG 2 cut(s) 120, 476
GluI GCNGC 3 cut(s) 60, 549, 758
GsaI CCCAGC 2 cut(s) 852, 972
GsuI CTGGAG 2 cut(s) 466, 670
HaeIII GGCC 4 cut(s) 59, 152, 185, 520
HapII CCGG 2 cut(s) 96, 480
Hin1II CATG 8 cut(s) 48, 167, 212, 233, 612, 793, 942, 956
HincII GTYRAC 2 cut(s) 508, 543
HindII GTYRAC 2 cut(s) 508, 543
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 3 cut(s) 12, 393, 773
HpaII CCGG 2 cut(s) 96, 480
HphI GGTGA 4 cut(s) 249, 329, 590, 893
Hpy166II GTNNAC 6 cut(s) 104, 286, 434, 508, 543, 769
Hpy188I TCNGA 3 cut(s) 392, 398, 636
Hpy188III TCNNGA 6 cut(s) 225, 329, 445, 692, 785, 835
Hpy8I GTNNAC 6 cut(s) 104, 286, 434, 508, 543, 769
HpyAV CCTTC 3 cut(s) 264, 657, 851
HpyCH4III ACNGT 2 cut(s) 346, 492
HpyCH4V TGCA 3 cut(s) 236, 718, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 242
HpyF3I CTNAG 4 cut(s) 131, 336, 633, 707
Hsp92II CATG 8 cut(s) 48, 167, 212, 233, 612, 793, 942, 956
Kzo9I GATC 4 cut(s) 325, 331, 398, 574
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 4 cut(s) 121, 160, 482, 502
Lsp1109I GCAGC 2 cut(s) 535, 769
LweI GCATC 2 cut(s) 223, 705
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 2 cut(s) 120, 476
MaeIII GTNAC 2 cut(s) 785, 957
MalI GATC 4 cut(s) 327, 333, 400, 576
MboI GATC 4 cut(s) 325, 331, 398, 574
MboII GAAGA 8 cut(s) 153, 213, 284, 361, 383, 446, 724, 899
MflI RGATCY 2 cut(s) 331, 398
MhlI GDGCHC 2 cut(s) 586, 975
MlsI TGGCCA 2 cut(s) 185, 520
MluCI AATT 2 cut(s) 404, 638
MluNI TGGCCA 2 cut(s) 185, 520
MlyI GAGTC 2 cut(s) 21, 782
MmeI TCCRAC 2 cut(s) 13, 397
MnlI CCTC 2 cut(s) 84, 477
Mox20I TGGCCA 2 cut(s) 185, 520
MscI TGGCCA 2 cut(s) 185, 520
MseI TTAA 2 cut(s) 945, 1013
MslI CAYNNNNRTG 1 cut(s) 622
Msp20I TGGCCA 2 cut(s) 185, 520
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 2 cut(s) 96, 480
MspR9I CCNGG 5 cut(s) 96, 309, 522, 570, 963
MvaI CCWGG 4 cut(s) 309, 522, 570, 963
MwoI GCNNNNNNNGC 2 cut(s) 56, 242
NciI CCSGG 1 cut(s) 96
NcoI CCATGG 2 cut(s) 608, 938
NdeII GATC 4 cut(s) 325, 331, 398, 574
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 8 cut(s) 48, 167, 212, 233, 612, 793, 942, 956
NlaIV GGNNCC 3 cut(s) 91, 400, 862
NmuCI GTSAC 1 cut(s) 785
PasI CCCWGGG 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 393
PflMI CCANNNNNTGG 2 cut(s) 314, 968
PkrI GCNGC 3 cut(s) 61, 550, 759
PleI GAGTC 2 cut(s) 20, 781
PpsI GAGTC 2 cut(s) 20, 781
Psp6I CCWGG 4 cut(s) 307, 520, 568, 961
PspFI CCCAGC 2 cut(s) 848, 968
PspGI CCWGG 4 cut(s) 307, 520, 568, 961
PspN4I GGNNCC 3 cut(s) 91, 400, 862
PspPI GGNCC 1 cut(s) 861
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 2 cut(s) 331, 398
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 2 cut(s) 285, 461
RsaNI GTAC 2 cut(s) 284, 460
RseI CAYNNNNRTG 1 cut(s) 622
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 2 cut(s) 945, 1013
SatI GCNGC 3 cut(s) 60, 549, 758
Sau3AI GATC 4 cut(s) 325, 331, 398, 574
Sau96I GGNCC 1 cut(s) 861
SchI GAGTC 2 cut(s) 21, 782
ScrFI CCNGG 5 cut(s) 96, 309, 522, 570, 963
SduI GDGCHC 2 cut(s) 586, 975
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 2 cut(s) 223, 705
SinI GGWCC 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 622
Sse9I AATT 2 cut(s) 404, 638
SsiI CCGC 3 cut(s) 60, 68, 87
SspMI CTAG 2 cut(s) 120, 476
StyD4I CCNGG 5 cut(s) 94, 307, 520, 568, 961
StyI CCWWGG 2 cut(s) 608, 938
TaaI ACNGT 2 cut(s) 346, 492
TaqI TCGA 2 cut(s) 589, 776
TasI AATT 2 cut(s) 404, 638
TatI WGTACW 1 cut(s) 459
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 2 cut(s) 945, 1013
Tru9I TTAA 2 cut(s) 945, 1013
TscAI CASTG 3 cut(s) 243, 351, 495
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 2 cut(s) 548, 757
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 6 cut(s) 197, 639, 656, 870, 900, 1017
TspGWI ACGGA 2 cut(s) 765, 853
TspRI CASTG 3 cut(s) 243, 351, 495
Van91I CCANNNNNTGG 2 cut(s) 314, 968
VpaK11BI GGWCC 1 cut(s) 861
XapI RAATTY 1 cut(s) 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 2 cut(s) 120, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.