MD07G1064600.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000873
Physical Location & Seq
Reverse (-)
6056233 .. 6062979
6747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1064600.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGAATTTTCTTCGTTACTTCTCTATCTTCTTCGTTTGTCAATACGAAAACAAGCAGCCCACCGACACCGGCAGCTTCAGCACCACACAAAAGCTAAAAGCAGCTGCTCACTCAGCCCGTTTCTGCCATTTTCTTCTGCATAAACATATAATTATCACAGAGGGAGGAGCGTTGGTGGATCACATTCATCTTGTTGGTTGGATTGCTGGTGTGGGCGTATCAGCCGCCCGAACACCCCCTCCGAACATCTGTGGTTCGCCTGGCAGTCCACCCGTCACATCGCCTAGAATAAGGCTAAGGGATGGAAGATTTTTGGCCTACAAGGAACATGGTGTTCCAAAACAAGTGGCCAAGTATAACATTATCTTTGTTCATCCCTTCGGTAGTTCCCGACATGGTCCAGTCATTGCAACATCTGTCTCTCCGGAAGTTATTGAAGATTTAGGTGTGTACATTGTGTCTTTCGACAGACCAGGGTACGGTGAAAGTGATCCAGATCCTAAGCGAACAGCGAAGAGATTGGCGTTAGATATAGAGGAGCTTGGTGATCAGCTGGAACTCGGATCCAAATTTTATGTAATTGGATACTCAATGGGAGGACAGGCCATTTGGAAGTGTCTCCAGTACATCCCTCATAGGATGTGGCCAGGACTTCCTGCAAACTTGTCATCCTATGCCTATAAGAAGCAACTTCCACAAGATCAATGGGCGGTTCGAGTTGCTCACTATATACCTTGGTTAACCTACTGGTGGAACACTCAGAAATTCTTTCCTTCATCCAGCGTTGTATCCGGTAAACCAGAGAATGTGTTTTCCCGCCAAGATTGGGAAATCATTAGGAATGCAAAGGTTTCTGGAAGAGAAAAGCACAAGGCATATGTCACGCAGCAAGGAGAAGCTGAGTCCATCTTACGCGACATGGTAGTTGGATTTGGGAGCTGGGAATTTGATCCTATGGATCTGCAGAACCCTTTTGCCAGCAACCAAGGCTCAGTCCATCTGTGGCAGGGTGATGAAGATAAGCTTGTCCAAGTTGAGCTGCAACGCTATATTGCCGAAACGCTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

357

Amino Acids

40.22

Weight (kDa)

8.51

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 121 - 205 4.3e-07 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 121 - 213 1.5e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 915
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 3 cut(s) 225, 710, 817
AclWI GGATC 7 cut(s) 186, 485, 491, 558, 571, 944, 966
AcoI YGGCCR 2 cut(s) 348, 644
AcsI RAATTY 4 cut(s) 4, 569, 764, 944
AcuI CTGAAG 1 cut(s) 61
AfaI GTAC 3 cut(s) 452, 479, 626
AfiI CCNNNNNNNGG 3 cut(s) 479, 750, 826
AgsI TTSAA 1 cut(s) 437
AjnI CCWGG 3 cut(s) 259, 472, 646
AloI GAACNNNNNNTCC 2 cut(s) 223, 255
AluBI AGCT 9 cut(s) 75, 94, 104, 541, 553, 899, 939, 1024, 1039
AluI AGCT 9 cut(s) 75, 94, 104, 541, 553, 899, 939, 1024, 1039
Alw26I GTCTC 2 cut(s) 424, 623
AlwI GGATC 7 cut(s) 186, 485, 491, 558, 571, 944, 966
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 4 cut(s) 315, 348, 603, 644
ApeKI GCWGC 6 cut(s) 55, 72, 101, 104, 886, 1039
ApoI RAATTY 4 cut(s) 4, 569, 764, 944
ArsI GACNNNNNNTTYG 2 cut(s) 591, 623
Asp700I GAANNNNTTC 1 cut(s) 1062
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 3 cut(s) 494, 557, 1022
AvaII GGWCC 1 cut(s) 398
BalI TGGCCA 2 cut(s) 350, 646
BamHI GGATCC 1 cut(s) 563
BbvI GCAGC 6 cut(s) 67, 84, 91, 113, 898, 1026
BccI CCATC 3 cut(s) 296, 914, 1005
BciT130I CCWGG 3 cut(s) 261, 474, 648
BciVI GTATCC 2 cut(s) 578, 799
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 424, 623
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 962
BfuI GTATCC 2 cut(s) 578, 799
BisI GCNGC 7 cut(s) 56, 73, 102, 105, 225, 887, 1040
BlsI GCNGC 7 cut(s) 57, 74, 103, 106, 226, 888, 1041
Bme1390I CCNGG 3 cut(s) 261, 474, 648
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 3 cut(s) 261, 474, 648
BpmI CTGGAG 1 cut(s) 605
Bpu10I CCTNAGC 2 cut(s) 296, 501
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 3 cut(s) 473, 734, 985
BsaWI WCCGGW 2 cut(s) 424, 791
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 223, 253
Bsc4I CCNNNNNNNGG 3 cut(s) 479, 750, 826
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 3 cut(s) 401, 622, 752
Bse3DI GCAATG 1 cut(s) 405
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 3 cut(s) 261, 474, 648
BseDI CCNNGG 3 cut(s) 473, 734, 985
BseGI GGATG 6 cut(s) 307, 373, 627, 645, 668, 776
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 3 cut(s) 479, 750, 826
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 4 cut(s) 126, 773, 891, 1005
BseNI ACTGG 3 cut(s) 401, 622, 752
BseRI GAGGAG 2 cut(s) 180, 551
BseXI GCAGC 6 cut(s) 67, 84, 91, 113, 898, 1026
BseYI CCCAGC 1 cut(s) 939
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 4 cut(s) 317, 350, 605, 646
BsiSI CCGG 3 cut(s) 69, 425, 792
BslI CCNNNNNNNGG 3 cut(s) 479, 750, 826
BsmAI GTCTC 2 cut(s) 424, 623
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 4 cut(s) 317, 350, 605, 646
Bsp13I TCCGGA 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
BspACI CCGC 3 cut(s) 225, 710, 817
BspANI GGCC 4 cut(s) 317, 350, 605, 646
BspCNI CTCAG 4 cut(s) 125, 772, 892, 1004
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 1 cut(s) 915
BspLI GGNNCC 1 cut(s) 565
BspMAI CTGCAG 1 cut(s) 966
BspPI GGATC 7 cut(s) 186, 485, 491, 558, 571, 944, 966
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 3 cut(s) 401, 622, 752
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 3 cut(s) 473, 734, 985
BssMI GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
BssT1I CCWWGG 2 cut(s) 734, 985
Bst2UI CCWGG 3 cut(s) 261, 474, 648
Bst4CI ACNGT 1 cut(s) 482
Bst6I CTCTTC 2 cut(s) 509, 853
BstAUI TGTACA 1 cut(s) 450
BstC8I GCNNGC 1 cut(s) 979
BstDEI CTNAG 6 cut(s) 112, 296, 501, 759, 900, 991
BstF5I GGATG 6 cut(s) 307, 373, 627, 645, 668, 776
BstFNI CGCG 1 cut(s) 915
BstKTI GATC 8 cut(s) 181, 493, 499, 550, 566, 703, 952, 961
BstMAI GTCTC 2 cut(s) 424, 623
BstMBI GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
BstMWI GCNNNNNNNGC 3 cut(s) 78, 113, 987
BstNI CCWGG 3 cut(s) 261, 474, 648
BstSCI CCNGG 3 cut(s) 259, 472, 646
BstSFI CTRYAG 1 cut(s) 962
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 6 cut(s) 67, 84, 91, 113, 898, 1026
BstX2I RGATCY 3 cut(s) 496, 563, 958
BstYI RGATCY 3 cut(s) 496, 563, 958
BsuI GTATCC 2 cut(s) 578, 799
BsuRI GGCC 4 cut(s) 317, 350, 605, 646
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 6 cut(s) 307, 373, 627, 645, 668, 776
Cac8I GCNNGC 1 cut(s) 979
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 398
Csp6I GTAC 3 cut(s) 451, 478, 625
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 3 cut(s) 329, 395, 919
CviQI GTAC 3 cut(s) 451, 478, 625
DdeI CTNAG 6 cut(s) 112, 296, 501, 759, 900, 991
DpnI GATC 8 cut(s) 180, 492, 498, 549, 565, 702, 951, 960
DpnII GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
EaeI YGGCCR 2 cut(s) 348, 644
Eam1104I CTCTTC 2 cut(s) 509, 853
EarI CTCTTC 2 cut(s) 509, 853
Eco130I CCWWGG 2 cut(s) 734, 985
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 1 cut(s) 61
EcoRII CCWGG 3 cut(s) 259, 472, 646
EcoT14I CCWWGG 2 cut(s) 734, 985
ErhI CCWWGG 2 cut(s) 734, 985
FaeI CATG 3 cut(s) 332, 398, 922
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FatI CATG 3 cut(s) 328, 394, 918
FauI CCCGC 1 cut(s) 824
FauNDI CATATG 1 cut(s) 877
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 7 cut(s) 56, 73, 102, 105, 225, 887, 1040
FokI GGATG 6 cut(s) 314, 360, 614, 652, 655, 763
Fsp4HI GCNGC 7 cut(s) 56, 73, 102, 105, 225, 887, 1040
FspBI CTAG 1 cut(s) 285
GluI GCNGC 7 cut(s) 56, 73, 102, 105, 225, 887, 1040
GsaI CCCAGC 1 cut(s) 943
GsuI CTGGAG 1 cut(s) 605
HaeIII GGCC 4 cut(s) 317, 350, 605, 646
HapII CCGG 3 cut(s) 69, 425, 792
Hin1II CATG 3 cut(s) 332, 398, 922
HincII GTYRAC 1 cut(s) 741
HindII GTYRAC 1 cut(s) 741
HindIII AAGCTT 1 cut(s) 1022
HinfI GANTC 1 cut(s) 902
HpaI GTTAAC 1 cut(s) 741
HpaII CCGG 3 cut(s) 69, 425, 792
HphI GGTGA 3 cut(s) 494, 557, 1022
Hpy166II GTNNAC 4 cut(s) 269, 451, 741, 797
Hpy188I TCNGA 3 cut(s) 243, 563, 762
Hpy188III TCNNGA 4 cut(s) 390, 425, 494, 855
Hpy8I GTNNAC 4 cut(s) 269, 451, 741, 797
HpyAV CCTTC 2 cut(s) 388, 783
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4V TGCA 6 cut(s) 139, 410, 659, 845, 964, 1042
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 113, 987
HpyF3I CTNAG 6 cut(s) 112, 296, 501, 759, 900, 991
Hsp92II CATG 3 cut(s) 332, 398, 922
Kpn2I TCCGGA 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 741
Kzo9I GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
LmnI GCTCC 3 cut(s) 167, 538, 936
Lsp1109I GCAGC 6 cut(s) 67, 84, 91, 113, 898, 1026
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 3 cut(s) 14, 274, 880
MalI GATC 8 cut(s) 180, 492, 498, 549, 565, 702, 951, 960
MboI GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
MboII GAAGA 8 cut(s) 19, 22, 125, 318, 449, 526, 870, 1028
MflI RGATCY 3 cut(s) 496, 563, 958
MlsI TGGCCA 2 cut(s) 350, 646
MluCI AATT 6 cut(s) 4, 150, 569, 579, 764, 944
MluNI TGGCCA 2 cut(s) 350, 646
MlyI GAGTC 1 cut(s) 911
MmeI TCCRAC 2 cut(s) 179, 907
MnlI CCTC 6 cut(s) 154, 158, 249, 529, 590, 642
Mox20I TGGCCA 2 cut(s) 350, 646
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 2 cut(s) 350, 646
MseI TTAA 1 cut(s) 740
Msp20I TGGCCA 2 cut(s) 350, 646
MspA1I CMGCKG 2 cut(s) 104, 553
MspI CCGG 3 cut(s) 69, 425, 792
MspR9I CCNGG 3 cut(s) 261, 474, 648
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 3 cut(s) 261, 474, 648
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 3 cut(s) 78, 113, 987
NdeI CATATG 1 cut(s) 877
NdeII GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
NlaIII CATG 3 cut(s) 332, 398, 922
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 2 cut(s) 274, 880
PctI GAATGC 1 cut(s) 847
PdmI GAANNNNTTC 1 cut(s) 1062
PflFI GACNNNGTC 1 cut(s) 396
PkrI GCNGC 7 cut(s) 57, 74, 103, 106, 226, 888, 1041
PleI GAGTC 1 cut(s) 910
PpsI GAGTC 1 cut(s) 910
Psp6I CCWGG 3 cut(s) 259, 472, 646
PspFI CCCAGC 1 cut(s) 939
PspGI CCWGG 3 cut(s) 259, 472, 646
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 1 cut(s) 398
PstI CTGCAG 1 cut(s) 966
PsuI RGATCY 3 cut(s) 496, 563, 958
PsyI GACNNNGTC 1 cut(s) 396
PvuII CAGCTG 2 cut(s) 104, 553
RsaI GTAC 3 cut(s) 452, 479, 626
RsaNI GTAC 3 cut(s) 451, 478, 625
SaqAI TTAA 1 cut(s) 740
SatI GCNGC 7 cut(s) 56, 73, 102, 105, 225, 887, 1040
Sau3AI GATC 8 cut(s) 178, 490, 496, 547, 563, 700, 949, 958
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 1 cut(s) 911
ScrFI CCNGG 3 cut(s) 261, 474, 648
SfcI CTRYAG 1 cut(s) 962
SinI GGWCC 1 cut(s) 398
Sse9I AATT 6 cut(s) 4, 150, 569, 579, 764, 944
SsiI CCGC 3 cut(s) 225, 710, 817
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 259, 472, 646
StyI CCWWGG 2 cut(s) 734, 985
TaaI ACNGT 1 cut(s) 482
TaqI TCGA 2 cut(s) 465, 715
TasI AATT 6 cut(s) 4, 150, 569, 579, 764, 944
TatI WGTACW 2 cut(s) 450, 624
TauI GCSGC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 740
Tru9I TTAA 1 cut(s) 740
TseFI GTSAC 2 cut(s) 274, 880
TseI GCWGC 6 cut(s) 55, 72, 101, 104, 886, 1039
Tsp45I GTSAC 2 cut(s) 274, 880
TspDTI ATGAA 5 cut(s) 17, 176, 362, 765, 1029
Tth111I GACNNNGTC 1 cut(s) 396
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 4 cut(s) 4, 569, 764, 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 702
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.