RchiOBHm_Chr3g0473031

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000873
Physical Location & Seq
Forward (+)
19021787 .. 19026425
4639 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43872

Sequence Viewer

Length: 1131 bp
ATGGCCGGTGGAGTTAACCGGAAGATATCGGCCGCTTCGGCCAGAGCTCACACCCGAAGAACCAAGCAAAACAGCTCCTTCCGTCTTCCTTCAGGGATGTTTAGGACAACACTGGTGGTGTTGTTTATGGGGTTTCTGGCATGGGCATATCAGGCCATCCAACCTCCTCCACCAAAGATTTGTGGCTCTCCTGATGGCCCTCCTGTTACAGCACCAAGAATAAAACTTAGGGATGGAAGGCATTTAGCCTATCAAGAGCATGGGGTCCCAAAAGAAAATGCTCAGTATAAAATTGTTTATGTCCATGGTATTGATTCTTGCAGACATGATGCTGTTGCTGCACAAACCCTATCTCCAGAAATTGTTGAAGACTTGGGTGTCTACTTTGTGTCTTTTGACAGACCTGGTTATGGAGAGAGTGATCCTGATCCAAATAGAACAGCAAAGAGTATGGCTTTAGATATAGAGGAGCTGGCTGATGAATTAGGATTAGGATCCAGATTCTATGTAATTGGTTTTTCCATGGGTGGACAGGTTCTTTGGGGTTGCCTCAAGTACATCCCTCACAGGCTAGCAGGAGCGGCACTATTAGCTCCAGTTGTTAATTACTGGTGGACCGGTATTCCTGCAAATCTATCTAACGAAGCATACCACCAACAGTTTCAGCAAGACCAGTGGGCAGTTCGTGTTTCTCACTACACCCCTTGGCTTACCTATTTCTGGAACACTCAAAAATGGTTTCCCGCTTCAAGTGTTATGGCTCACAGCACAGATATTCTTTCTCAACAAGACAAAGAACTCCTCCCTAAGCTTTTCAAAAGAAAACCATATATGGACCAGGTAAGACAGCAAGGAGAATTCGAGTCCATTCACCGTGACATGAATCTTGGATTTGGGACCTGGGAATTCAGTCCTCTGGATCTGGAAAATCCTTTTCCAAACAATGAAGCTTCTGTTCATGTGTGGCATGGAGATGAAGATAACATGGTTCCTGTTACTCTGCAACGATACATTGCCCAACAACTTCCCTGGATTCAATATCATGAGATACCAGGTGCTGGACACTTGTTCCCACATGCTGATGGGATGTGCAATACCATTGTCAAGGCACTTTTAACTGATGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

376

Amino Acids

42.52

Weight (kDa)

6.43

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 98 - 360 6.8e-16 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 121 - 357 1.7e-13 alpha/beta hydrolase fold
Hydrolase_4 PF12146 123 - 356 2.3e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccBSI CCGCTC 1 cut(s) 581
AccI GTMKAC 1 cut(s) 381
AciI CCGC 3 cut(s) 33, 581, 744
AclWI GGATC 5 cut(s) 416, 422, 489, 502, 927
AcoI YGGCCR 3 cut(s) 3, 30, 39
AcsI RAATTY 2 cut(s) 857, 905
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 3 cut(s) 410, 720, 1058
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 4 cut(s) 368, 750, 817, 1037
AjnI CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
AluBI AGCT 6 cut(s) 47, 75, 472, 593, 811, 950
AluI AGCT 6 cut(s) 47, 75, 472, 593, 811, 950
Alw21I GWGCWC 1 cut(s) 49
AlwI GGATC 5 cut(s) 416, 422, 489, 502, 927
AlwNI CAGNNNCTG 1 cut(s) 1058
AoxI GGCC 5 cut(s) 3, 30, 39, 153, 196
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 2 cut(s) 857, 905
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
AsiGI ACCGGT 1 cut(s) 617
AspS9I GGNCC 5 cut(s) 197, 265, 615, 835, 897
AsuHPI GGTGA 1 cut(s) 863
AsuNHI GCTAGC 1 cut(s) 571
AvaII GGWCC 4 cut(s) 265, 615, 835, 897
BamHI GGATCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 49
BbsI GAAGAC 2 cut(s) 77, 375
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 325
BccI CCATC 4 cut(s) 164, 188, 227, 1076
BciT130I CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BfaI CTAG 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 3 cut(s) 33, 339, 582
BlsI GCNGC 3 cut(s) 34, 340, 583
Bme1390I CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
Bme18I GGWCC 4 cut(s) 265, 615, 835, 897
BmgT120I GGNCC 5 cut(s) 197, 265, 615, 835, 897
BmiI GGNNCC 5 cut(s) 266, 267, 496, 898, 990
BmrFI CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
BmsI GCATC 1 cut(s) 319
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 2 cut(s) 77, 375
BpmI CTGGAG 2 cut(s) 339, 579
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 536
BsaBI GATNNNNATC 2 cut(s) 426, 493
BsaJI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BsaWI WCCGGW 2 cut(s) 18, 617
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 720, 1058
Bse118I RCCGGY 2 cut(s) 5, 617
Bse1I ACTGG 4 cut(s) 117, 596, 614, 673
Bse3DI GCAATG 1 cut(s) 1011
Bse8I GATNNNNATC 2 cut(s) 426, 493
BseBI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BseDI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BseGI GGATG 5 cut(s) 102, 156, 238, 558, 1092
BseJI GATNNNNATC 2 cut(s) 426, 493
BseLI CCNNNNNNNGG 3 cut(s) 410, 720, 1058
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 4 cut(s) 117, 596, 614, 673
BseRI GAGGAG 3 cut(s) 156, 482, 791
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 325
BsgI GTGCAG 1 cut(s) 324
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 5, 32, 41, 155, 198
BshTI ACCGGT 1 cut(s) 617
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 3 cut(s) 6, 19, 618
BslFI GGGAC 2 cut(s) 251, 910
BslI CCNNNNNNNGG 3 cut(s) 410, 720, 1058
BsmFI GGGAC 2 cut(s) 251, 910
BsnI GGCC 5 cut(s) 5, 32, 41, 155, 198
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 4 cut(s) 421, 427, 494, 919
Bsp19I CCATGG 2 cut(s) 304, 522
BspACI CCGC 3 cut(s) 33, 581, 744
BspANI GGCC 5 cut(s) 5, 32, 41, 155, 198
BspCNI CTCAG 1 cut(s) 295
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 5 cut(s) 266, 267, 496, 898, 990
BspOI GCTAGC 1 cut(s) 575
BspPI GGATC 5 cut(s) 416, 422, 489, 502, 927
BsrBI CCGCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 1011
BsrFI RCCGGY 2 cut(s) 5, 617
BsrI ACTGG 4 cut(s) 117, 596, 614, 673
BssAI RCCGGY 2 cut(s) 5, 617
BssECI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BssMI GATC 4 cut(s) 421, 427, 494, 919
BssT1I CCWWGG 3 cut(s) 304, 522, 704
Bst2UI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
Bst4CI ACNGT 2 cut(s) 660, 875
BstC8I GCNNGC 2 cut(s) 474, 573
BstDEI CTNAG 3 cut(s) 227, 282, 807
BstDSI CCRYGG 2 cut(s) 304, 522
BstF5I GGATG 5 cut(s) 102, 156, 238, 558, 1092
BstKTI GATC 4 cut(s) 424, 430, 497, 922
BstMBI GATC 4 cut(s) 421, 427, 494, 919
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 5 cut(s) 38, 152, 338, 581, 590
BstNI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BstNSI RCATGY 1 cut(s) 1079
BstSCI CCNGG 5 cut(s) 403, 837, 899, 1028, 1051
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 2 cut(s) 77, 375
BstX2I RGATCY 2 cut(s) 494, 919
BstYI RGATCY 2 cut(s) 494, 919
BstZI CGGCCG 1 cut(s) 30
BsuRI GGCC 5 cut(s) 5, 32, 41, 155, 198
BtgI CCRYGG 2 cut(s) 304, 522
BtsCI GGATG 5 cut(s) 102, 156, 238, 558, 1092
BtsIMutI CAGTG 2 cut(s) 110, 680
Cac8I GCNNGC 2 cut(s) 474, 573
CaiI CAGNNNCTG 1 cut(s) 1058
CciI TCATGA 1 cut(s) 1042
Cfr10I RCCGGY 2 cut(s) 5, 617
Cfr13I GGNCC 5 cut(s) 197, 265, 615, 835, 897
CsiI ACCWGGT 3 cut(s) 403, 837, 1051
Csp6I GTAC 1 cut(s) 556
CspAI ACCGGT 1 cut(s) 617
CspCI CAANNNNNGTGG 4 cut(s) 96, 131, 656, 691
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 3 cut(s) 227, 282, 807
DpnI GATC 4 cut(s) 423, 429, 496, 921
DpnII GATC 4 cut(s) 421, 427, 494, 919
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 3 cut(s) 3, 30, 39
EagI CGGCCG 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 47
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 304, 522, 704
Eco24I GRGCYC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 4 cut(s) 265, 615, 835, 897
Eco52I CGGCCG 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 47
EcoO109I RGGNCCY 2 cut(s) 265, 897
EcoRI GAATTC 2 cut(s) 857, 905
EcoRII CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 3 cut(s) 304, 522, 704
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 3 cut(s) 304, 522, 704
FaqI GGGAC 2 cut(s) 251, 910
FauI CCCGC 1 cut(s) 751
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 33, 339, 582
FokI GGATG 5 cut(s) 109, 143, 245, 545, 1099
FriOI GRGCYC 1 cut(s) 49
Fsp4HI GCNGC 3 cut(s) 33, 339, 582
FspBI CTAG 1 cut(s) 572
GluI GCNGC 3 cut(s) 33, 339, 582
GsuI CTGGAG 2 cut(s) 339, 579
HaeIII GGCC 5 cut(s) 5, 32, 41, 155, 198
HapII CCGG 3 cut(s) 6, 19, 618
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 2 cut(s) 809, 948
HinfI GANTC 5 cut(s) 314, 501, 863, 883, 1033
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 3 cut(s) 6, 19, 618
HphI GGTGA 1 cut(s) 863
Hpy166II GTNNAC 4 cut(s) 16, 382, 530, 615
Hpy188III TCNNGA 9 cut(s) 191, 254, 356, 425, 498, 721, 917, 923, 1043
Hpy8I GTNNAC 4 cut(s) 16, 382, 530, 615
HpyAV CCTTC 3 cut(s) 88, 99, 231
HpyCH4III ACNGT 2 cut(s) 660, 875
HpyCH4V TGCA 5 cut(s) 321, 341, 629, 1003, 1092
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 152, 338, 581, 590
HpyF3I CTNAG 3 cut(s) 227, 282, 807
KflI GGGWCCC 1 cut(s) 265
KspAI GTTAAC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 421, 427, 494, 919
LmnI GCTCC 4 cut(s) 80, 469, 578, 598
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 319
MabI ACCWGGT 3 cut(s) 403, 837, 1051
MaeI CTAG 1 cut(s) 572
MaeIII GTNAC 3 cut(s) 205, 875, 994
MalI GATC 4 cut(s) 423, 429, 496, 921
MbiI CCGCTC 1 cut(s) 581
MboI GATC 4 cut(s) 421, 427, 494, 919
MboII GAAGA 5 cut(s) 34, 69, 77, 380, 989
MflI RGATCY 2 cut(s) 494, 919
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 7 cut(s) 291, 360, 482, 510, 604, 857, 905
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 8 cut(s) 174, 177, 210, 460, 560, 573, 812, 924
MseI TTAA 3 cut(s) 15, 603, 1115
MslI CAYNNNNRTG 2 cut(s) 972, 1080
MspI CCGG 3 cut(s) 6, 19, 618
MspR9I CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
MvaI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
MwoI GCNNNNNNNGC 5 cut(s) 38, 152, 338, 581, 590
NcoI CCATGG 2 cut(s) 304, 522
NdeII GATC 4 cut(s) 421, 427, 494, 919
NheI GCTAGC 1 cut(s) 571
NlaIV GGNNCC 5 cut(s) 266, 267, 496, 898, 990
NmuCI GTSAC 1 cut(s) 875
NspI RCATGY 1 cut(s) 1079
PagI TCATGA 1 cut(s) 1042
PfeI GAWTC 4 cut(s) 314, 501, 883, 1033
PflMI CCANNNNNTGG 1 cut(s) 1058
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 3 cut(s) 34, 340, 583
PleI GAGTC 1 cut(s) 871
PpsI GAGTC 1 cut(s) 871
PpuMI RGGWCCY 2 cut(s) 265, 897
Psp124BI GAGCTC 1 cut(s) 49
Psp5II RGGWCCY 2 cut(s) 265, 897
Psp6I CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
PspGI CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
PspN4I GGNNCC 5 cut(s) 266, 267, 496, 898, 990
PspPI GGNCC 5 cut(s) 197, 265, 615, 835, 897
PspPPI RGGWCCY 2 cut(s) 265, 897
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 2 cut(s) 494, 919
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
RseI CAYNNNNRTG 2 cut(s) 972, 1080
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 15, 603, 1115
SatI GCNGC 3 cut(s) 33, 339, 582
Sau3AI GATC 4 cut(s) 421, 427, 494, 919
Sau96I GGNCC 5 cut(s) 197, 265, 615, 835, 897
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
SduI GDGCHC 1 cut(s) 49
SexAI ACCWGGT 3 cut(s) 403, 837, 1051
SfaNI GCATC 1 cut(s) 319
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SinI GGWCC 4 cut(s) 265, 615, 835, 897
SmiMI CAYNNNNRTG 2 cut(s) 972, 1080
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 7 cut(s) 291, 360, 482, 510, 604, 857, 905
SsiI CCGC 3 cut(s) 33, 581, 744
SspMI CTAG 1 cut(s) 572
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 5 cut(s) 403, 837, 899, 1028, 1051
StyI CCWWGG 3 cut(s) 304, 522, 704
TaaI ACNGT 2 cut(s) 660, 875
TaqI TCGA 1 cut(s) 861
TasI AATT 7 cut(s) 291, 360, 482, 510, 604, 857, 905
TatI WGTACW 1 cut(s) 555
TauI GCSGC 2 cut(s) 35, 584
TfiI GAWTC 4 cut(s) 314, 501, 883, 1033
Tru1I TTAA 3 cut(s) 15, 603, 1115
Tru9I TTAA 3 cut(s) 15, 603, 1115
TscAI CASTG 2 cut(s) 117, 680
TseFI GTSAC 1 cut(s) 875
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 875
TspDTI ATGAA 5 cut(s) 495, 896, 947, 960, 990
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 2 cut(s) 117, 680
Van91I CCANNNNNTGG 1 cut(s) 1058
VpaK11BI GGWCC 4 cut(s) 265, 615, 835, 897
XapI RAATTY 2 cut(s) 857, 905
XceI RCATGY 1 cut(s) 1079
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.