Alpha/beta hydrolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 964 |
| AccB1I | GGYRCC | 1 cut(s) | 964 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 974 |
| AccIII | TCCGGA | 3 cut(s) | 262, 331, 668 |
| AciI | CCGC | 4 cut(s) | 63, 102, 572, 694 |
| AclWI | GGATC | 9 cut(s) | 23, 230, 323, 329, 342, 396, 409, 585, 843 |
| AcoI | YGGCCR | 2 cut(s) | 186, 521 |
| AcsI | RAATTY | 3 cut(s) | 407, 640, 821 |
| AcuI | CTGAAG | 2 cut(s) | 443, 860 |
| AfaI | GTAC | 5 cut(s) | 223, 290, 317, 464, 966 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 317, 627, 703, 974 |
| AflIII | ACRYGT | 1 cut(s) | 754 |
| AgsI | TTSAA | 1 cut(s) | 275 |
| AjnI | CCWGG | 4 cut(s) | 97, 310, 523, 967 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 855, 887 |
| Alw26I | GTCTC | 1 cut(s) | 262 |
| AlwI | GGATC | 9 cut(s) | 23, 230, 323, 329, 342, 396, 409, 585, 843 |
| Aor13HI | TCCGGA | 3 cut(s) | 262, 331, 668 |
| AoxI | GGCC | 4 cut(s) | 60, 153, 186, 521 |
| ApeKI | GCWGC | 4 cut(s) | 596, 763, 916, 992 |
| ApoI | RAATTY | 3 cut(s) | 407, 640, 821 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 727, 939 |
| Asp718I | GGTACC | 1 cut(s) | 964 |
| AspLEI | GCGC | 2 cut(s) | 121, 589 |
| AspS9I | GGNCC | 3 cut(s) | 104, 827, 871 |
| AsuHPI | GGTGA | 4 cut(s) | 332, 395, 517, 899 |
| AsuNHI | GCTAGC | 1 cut(s) | 478 |
| AvaII | GGWCC | 3 cut(s) | 104, 827, 871 |
| BaeGI | GKGCMC | 1 cut(s) | 55 |
| BalI | TGGCCA | 2 cut(s) | 188, 523 |
| BamHI | GGATCC | 2 cut(s) | 334, 401 |
| BanI | GGYRCC | 1 cut(s) | 964 |
| BbsI | GAAGAC | 1 cut(s) | 449 |
| BbvI | GCAGC | 4 cut(s) | 583, 775, 903, 979 |
| BccI | CCATC | 4 cut(s) | 134, 791, 882, 992 |
| BciT130I | CCWGG | 4 cut(s) | 99, 312, 525, 969 |
| BciVI | GTATCC | 2 cut(s) | 416, 676 |
| BclI | TGATCA | 2 cut(s) | 385, 507 |
| BcoDI | GTCTC | 1 cut(s) | 262 |
| BfaI | CTAG | 2 cut(s) | 123, 479 |
| BfmI | CTRYAG | 1 cut(s) | 993 |
| BfoI | RGCGCY | 2 cut(s) | 122, 590 |
| BfuI | GTATCC | 2 cut(s) | 416, 676 |
| BisI | GCNGC | 5 cut(s) | 63, 597, 764, 917, 993 |
| BlsI | GCNGC | 5 cut(s) | 64, 598, 765, 918, 994 |
| Bme1390I | CCNGG | 4 cut(s) | 99, 312, 525, 969 |
| Bme18I | GGWCC | 3 cut(s) | 104, 827, 871 |
| BmgT120I | GGNCC | 3 cut(s) | 104, 827, 871 |
| BmiI | GGNNCC | 3 cut(s) | 336, 403, 966 |
| BmrFI | CCNGG | 4 cut(s) | 99, 312, 525, 969 |
| BmrI | ACTGGG | 1 cut(s) | 902 |
| BmsI | GCATC | 1 cut(s) | 453 |
| BmtI | GCTAGC | 1 cut(s) | 482 |
| BmuI | ACTGGG | 1 cut(s) | 902 |
| BpiI | GAAGAC | 1 cut(s) | 449 |
| Bpu10I | CCTNAGC | 2 cut(s) | 134, 339 |
| BsaBI | GATNNNNATC | 1 cut(s) | 333 |
| BsaJI | CCNNGG | 3 cut(s) | 311, 611, 944 |
| BsaWI | WCCGGW | 4 cut(s) | 262, 331, 502, 668 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 317, 627, 703, 974 |
| Bse1I | ACTGG | 4 cut(s) | 239, 521, 629, 908 |
| Bse3DI | GCAATG | 2 cut(s) | 243, 865 |
| Bse8I | GATNNNNATC | 1 cut(s) | 333 |
| BseAI | TCCGGA | 3 cut(s) | 262, 331, 668 |
| BseBI | CCWGG | 4 cut(s) | 99, 312, 525, 969 |
| BseDI | CCNNGG | 3 cut(s) | 311, 611, 944 |
| BseGI | GGATG | 3 cut(s) | 145, 211, 465 |
| BseJI | GATNNNNATC | 1 cut(s) | 333 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 317, 627, 703, 974 |
| BseMI | GCAATG | 2 cut(s) | 243, 865 |
| BseMII | CTCAG | 2 cut(s) | 650, 768 |
| BseNI | ACTGG | 4 cut(s) | 239, 521, 629, 908 |
| BseRI | GAGGAG | 1 cut(s) | 389 |
| BseSI | GKGCMC | 1 cut(s) | 55 |
| BseXI | GCAGC | 4 cut(s) | 583, 775, 903, 979 |
| BseYI | CCCAGC | 1 cut(s) | 816 |
| BshFI | GGCC | 4 cut(s) | 62, 155, 188, 523 |
| BshNI | GGYRCC | 1 cut(s) | 964 |
| BsiSI | CCGG | 4 cut(s) | 263, 332, 503, 669 |
| BslFI | GGGAC | 1 cut(s) | 890 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 317, 627, 703, 974 |
| BsmAI | GTCTC | 1 cut(s) | 262 |
| BsmFI | GGGAC | 1 cut(s) | 890 |
| BsmI | GAATGC | 1 cut(s) | 20 |
| BsnI | GGCC | 4 cut(s) | 62, 155, 188, 523 |
| Bsp1286I | GDGCHC | 1 cut(s) | 55 |
| Bsp13I | TCCGGA | 3 cut(s) | 262, 331, 668 |
| Bsp1407I | TGTACA | 1 cut(s) | 288 |
| Bsp19I | CCATGG | 1 cut(s) | 944 |
| BspACI | CCGC | 4 cut(s) | 63, 102, 572, 694 |
| BspANI | GGCC | 4 cut(s) | 62, 155, 188, 523 |
| BspCNI | CTCAG | 2 cut(s) | 649, 769 |
| BspEI | TCCGGA | 3 cut(s) | 262, 331, 668 |
| BspLI | GGNNCC | 3 cut(s) | 336, 403, 966 |
| BspMAI | CTGCAG | 1 cut(s) | 997 |
| BspOI | GCTAGC | 1 cut(s) | 482 |
| BspPI | GGATC | 9 cut(s) | 23, 230, 323, 329, 342, 396, 409, 585, 843 |
| BspQI | GCTCTTC | 1 cut(s) | 347 |
| BspT107I | GGYRCC | 1 cut(s) | 964 |
| BsrDI | GCAATG | 2 cut(s) | 243, 865 |
| BsrGI | TGTACA | 1 cut(s) | 288 |
| BsrI | ACTGG | 4 cut(s) | 239, 521, 629, 908 |
| BssECI | CCNNGG | 3 cut(s) | 311, 611, 944 |
| BssT1I | CCWWGG | 2 cut(s) | 611, 944 |
| Bst2UI | CCWGG | 4 cut(s) | 99, 312, 525, 969 |
| Bst4CI | ACNGT | 2 cut(s) | 320, 441 |
| Bst6I | CTCTTC | 1 cut(s) | 347 |
| BstAUI | TGTACA | 1 cut(s) | 288 |
| BstC8I | GCNNGC | 4 cut(s) | 45, 480, 856, 990 |
| BstDEI | CTNAG | 4 cut(s) | 134, 339, 636, 777 |
| BstDSI | CCRYGG | 1 cut(s) | 944 |
| BstF5I | GGATG | 3 cut(s) | 145, 211, 465 |
| BstH2I | RGCGCY | 2 cut(s) | 122, 590 |
| BstHHI | GCGC | 2 cut(s) | 121, 589 |
| BstMAI | GTCTC | 1 cut(s) | 262 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 59, 571, 593, 864 |
| BstNI | CCWGG | 4 cut(s) | 99, 312, 525, 969 |
| BstNSI | RCATGY | 1 cut(s) | 758 |
| BstSCI | CCNGG | 4 cut(s) | 97, 310, 523, 967 |
| BstSFI | CTRYAG | 1 cut(s) | 993 |
| BstSLI | GKGCMC | 1 cut(s) | 55 |
| BstV1I | GCAGC | 4 cut(s) | 583, 775, 903, 979 |
| BstV2I | GAAGAC | 1 cut(s) | 449 |
| BstX2I | RGATCY | 3 cut(s) | 334, 401, 835 |
| BstYI | RGATCY | 3 cut(s) | 334, 401, 835 |
| BsuI | GTATCC | 2 cut(s) | 416, 676 |
| BsuRI | GGCC | 4 cut(s) | 62, 155, 188, 523 |
| BtgI | CCRYGG | 1 cut(s) | 944 |
| BtsCI | GGATG | 3 cut(s) | 145, 211, 465 |
| BtsI | GCAGTG | 1 cut(s) | 495 |
| BtsIMutI | CAGTG | 2 cut(s) | 446, 495 |
| Cac8I | GCNNGC | 4 cut(s) | 45, 480, 856, 990 |
| CfoI | GCGC | 2 cut(s) | 121, 589 |
| Cfr13I | GGNCC | 3 cut(s) | 104, 827, 871 |
| CsiI | ACCWGGT | 1 cut(s) | 967 |
| Csp6I | GTAC | 5 cut(s) | 222, 289, 316, 463, 965 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 165, 200 |
| CviAII | CATG | 6 cut(s) | 167, 233, 755, 796, 945, 959 |
| CviQI | GTAC | 5 cut(s) | 222, 289, 316, 463, 965 |
| DdeI | CTNAG | 4 cut(s) | 134, 339, 636, 777 |
| EaeI | YGGCCR | 2 cut(s) | 186, 521 |
| Eam1104I | CTCTTC | 1 cut(s) | 347 |
| EarI | CTCTTC | 1 cut(s) | 347 |
| Eco130I | CCWWGG | 2 cut(s) | 611, 944 |
| Eco47I | GGWCC | 3 cut(s) | 104, 827, 871 |
| Eco57I | CTGAAG | 2 cut(s) | 443, 860 |
| EcoRII | CCWGG | 4 cut(s) | 97, 310, 523, 967 |
| EcoT14I | CCWWGG | 2 cut(s) | 611, 944 |
| ErhI | CCWWGG | 2 cut(s) | 611, 944 |
| FaeI | CATG | 6 cut(s) | 170, 236, 758, 799, 948, 962 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 188, 220, 885, 917 |
| FaqI | GGGAC | 1 cut(s) | 890 |
| FatI | CATG | 6 cut(s) | 166, 232, 754, 795, 944, 958 |
| FauI | CCCGC | 1 cut(s) | 701 |
| FbaI | TGATCA | 2 cut(s) | 385, 507 |
| Fnu4HI | GCNGC | 5 cut(s) | 63, 597, 764, 917, 993 |
| FokI | GGATG | 3 cut(s) | 152, 198, 452 |
| Fsp4HI | GCNGC | 5 cut(s) | 63, 597, 764, 917, 993 |
| FspBI | CTAG | 2 cut(s) | 123, 479 |
| GlaI | GCGC | 2 cut(s) | 120, 588 |
| GluI | GCNGC | 5 cut(s) | 63, 597, 764, 917, 993 |
| GsaI | CCCAGC | 1 cut(s) | 820 |
| HaeII | RGCGCY | 2 cut(s) | 122, 590 |
| HaeIII | GGCC | 4 cut(s) | 62, 155, 188, 523 |
| HapII | CCGG | 4 cut(s) | 263, 332, 503, 669 |
| HhaI | GCGC | 2 cut(s) | 121, 589 |
| Hin1II | CATG | 6 cut(s) | 170, 236, 758, 799, 948, 962 |
| Hin6I | GCGC | 2 cut(s) | 119, 587 |
| HinP1I | GCGC | 2 cut(s) | 119, 587 |
| HincII | GTYRAC | 1 cut(s) | 618 |
| HindII | GTYRAC | 1 cut(s) | 618 |
| HindIII | AAGCTT | 2 cut(s) | 899, 938 |
| HinfI | GANTC | 2 cut(s) | 779, 949 |
| HpaI | GTTAAC | 1 cut(s) | 618 |
| HpaII | CCGG | 4 cut(s) | 263, 332, 503, 669 |
| HphI | GGTGA | 4 cut(s) | 332, 395, 517, 899 |
| Hpy166II | GTNNAC | 4 cut(s) | 107, 289, 618, 977 |
| Hpy188I | TCNGA | 4 cut(s) | 81, 401, 639, 840 |
| Hpy188III | TCNNGA | 7 cut(s) | 228, 263, 332, 448, 669, 732, 791 |
| Hpy8I | GTNNAC | 4 cut(s) | 107, 289, 618, 977 |
| HpyAV | CCTTC | 1 cut(s) | 226 |
| HpyCH4III | ACNGT | 2 cut(s) | 320, 441 |
| HpyCH4V | TGCA | 4 cut(s) | 248, 444, 919, 995 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 59, 571, 593, 864 |
| HpyF3I | CTNAG | 4 cut(s) | 134, 339, 636, 777 |
| Hsp92II | CATG | 6 cut(s) | 170, 236, 758, 799, 948, 962 |
| HspAI | GCGC | 2 cut(s) | 119, 587 |
| Kpn2I | TCCGGA | 3 cut(s) | 262, 331, 668 |
| KpnI | GGTACC | 1 cut(s) | 968 |
| Ksp22I | TGATCA | 2 cut(s) | 385, 507 |
| KspAI | GTTAAC | 1 cut(s) | 618 |
| LguI | GCTCTTC | 1 cut(s) | 347 |
| LmnI | GCTCC | 4 cut(s) | 376, 485, 505, 813 |
| Lsp1109I | GCAGC | 4 cut(s) | 583, 775, 903, 979 |
| LweI | GCATC | 1 cut(s) | 453 |
| MabI | ACCWGGT | 1 cut(s) | 967 |
| MaeI | CTAG | 2 cut(s) | 123, 479 |
| MaeIII | GTNAC | 4 cut(s) | 112, 757, 791, 887 |
| MboII | GAAGA | 6 cut(s) | 156, 287, 364, 449, 853, 905 |
| MflI | RGATCY | 3 cut(s) | 334, 401, 835 |
| MhlI | GDGCHC | 1 cut(s) | 55 |
| MlsI | TGGCCA | 2 cut(s) | 188, 523 |
| MluCI | AATT | 4 cut(s) | 407, 640, 821, 1032 |
| MluNI | TGGCCA | 2 cut(s) | 188, 523 |
| MlyI | GAGTC | 1 cut(s) | 788 |
| MmeI | TCCRAC | 2 cut(s) | 16, 784 |
| MnlI | CCTC | 7 cut(s) | 87, 367, 428, 480, 579, 955, 1003 |
| Mox20I | TGGCCA | 2 cut(s) | 188, 523 |
| MroI | TCCGGA | 3 cut(s) | 262, 331, 668 |
| MroXI | GAANNNNTTC | 2 cut(s) | 727, 939 |
| MscI | TGGCCA | 2 cut(s) | 188, 523 |
| MseI | TTAA | 3 cut(s) | 9, 617, 1019 |
| MslI | CAYNNNNRTG | 1 cut(s) | 957 |
| Msp20I | TGGCCA | 2 cut(s) | 188, 523 |
| MspA1I | CMGCKG | 2 cut(s) | 391, 992 |
| MspI | CCGG | 4 cut(s) | 263, 332, 503, 669 |
| MspR9I | CCNGG | 4 cut(s) | 99, 312, 525, 969 |
| Mva1269I | GAATGC | 1 cut(s) | 20 |
| MvaI | CCWGG | 4 cut(s) | 99, 312, 525, 969 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 59, 571, 593, 864 |
| NcoI | CCATGG | 1 cut(s) | 944 |
| NheI | GCTAGC | 1 cut(s) | 478 |
| NlaIII | CATG | 6 cut(s) | 170, 236, 758, 799, 948, 962 |
| NlaIV | GGNNCC | 3 cut(s) | 336, 403, 966 |
| NmuCI | GTSAC | 4 cut(s) | 112, 757, 791, 887 |
| NspI | RCATGY | 1 cut(s) | 758 |
| PciI | ACATGT | 1 cut(s) | 754 |
| PciSI | GCTCTTC | 1 cut(s) | 347 |
| PctI | GAATGC | 1 cut(s) | 20 |
| PdmI | GAANNNNTTC | 2 cut(s) | 727, 939 |
| PfeI | GAWTC | 1 cut(s) | 949 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 974 |
| PkrI | GCNGC | 5 cut(s) | 64, 598, 765, 918, 994 |
| PleI | GAGTC | 1 cut(s) | 787 |
| PpsI | GAGTC | 1 cut(s) | 787 |
| PscI | ACATGT | 1 cut(s) | 754 |
| Psp6I | CCWGG | 4 cut(s) | 97, 310, 523, 967 |
| PspFI | CCCAGC | 1 cut(s) | 816 |
| PspGI | CCWGG | 4 cut(s) | 97, 310, 523, 967 |
| PspN4I | GGNNCC | 3 cut(s) | 336, 403, 966 |
| PspPI | GGNCC | 3 cut(s) | 104, 827, 871 |
| PstI | CTGCAG | 1 cut(s) | 997 |
| PsuI | RGATCY | 3 cut(s) | 334, 401, 835 |
| PvuII | CAGCTG | 2 cut(s) | 391, 992 |
| RsaI | GTAC | 5 cut(s) | 223, 290, 317, 464, 966 |
| RsaNI | GTAC | 5 cut(s) | 222, 289, 316, 463, 965 |
| RseI | CAYNNNNRTG | 1 cut(s) | 957 |
| SapI | GCTCTTC | 1 cut(s) | 347 |
| SaqAI | TTAA | 3 cut(s) | 9, 617, 1019 |
| SatI | GCNGC | 5 cut(s) | 63, 597, 764, 917, 993 |
| Sau96I | GGNCC | 3 cut(s) | 104, 827, 871 |
| SchI | GAGTC | 1 cut(s) | 788 |
| ScrFI | CCNGG | 4 cut(s) | 99, 312, 525, 969 |
| SduI | GDGCHC | 1 cut(s) | 55 |
| SexAI | ACCWGGT | 1 cut(s) | 967 |
| SfaNI | GCATC | 1 cut(s) | 453 |
| SfcI | CTRYAG | 1 cut(s) | 993 |
| SinI | GGWCC | 3 cut(s) | 104, 827, 871 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 957 |
| Sse9I | AATT | 4 cut(s) | 407, 640, 821, 1032 |
| SsiI | CCGC | 4 cut(s) | 63, 102, 572, 694 |
| SspMI | CTAG | 2 cut(s) | 123, 479 |
| StyD4I | CCNGG | 4 cut(s) | 97, 310, 523, 967 |
| StyI | CCWWGG | 2 cut(s) | 611, 944 |
| TaaI | ACNGT | 2 cut(s) | 320, 441 |
| TaqI | TCGA | 2 cut(s) | 303, 592 |
| TaqII | GACCGA | 1 cut(s) | 859 |
| TasI | AATT | 4 cut(s) | 407, 640, 821, 1032 |
| TatI | WGTACW | 2 cut(s) | 288, 462 |
| TauI | GCSGC | 1 cut(s) | 65 |
| TfiI | GAWTC | 1 cut(s) | 949 |
| Tru1I | TTAA | 3 cut(s) | 9, 617, 1019 |
| Tru9I | TTAA | 3 cut(s) | 9, 617, 1019 |
| TscAI | CASTG | 2 cut(s) | 446, 495 |
| TseFI | GTSAC | 4 cut(s) | 112, 757, 791, 887 |
| TseI | GCWGC | 4 cut(s) | 596, 763, 916, 992 |
| Tsp45I | GTSAC | 4 cut(s) | 112, 757, 791, 887 |
| TspDTI | ATGAA | 3 cut(s) | 13, 200, 876 |
| TspRI | CASTG | 2 cut(s) | 446, 495 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 974 |
| VpaK11BI | GGWCC | 3 cut(s) | 104, 827, 871 |
| XapI | RAATTY | 3 cut(s) | 407, 640, 821 |
| XceI | RCATGY | 1 cut(s) | 758 |
| XmnI | GAANNNNTTC | 2 cut(s) | 727, 939 |
| XspI | CTAG | 2 cut(s) | 123, 479 |