Rh1AG126200

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000873
Physical Location & Seq
Reverse (-)
23480934 .. 23483977
3044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG126200.1

Sequence Viewer

Length: 1041 bp
ATGTTTAGAAGAGTCGCATTTTTGTTGTTGGTTGGATTGCTAGCATGGGCTTATCAGGCCACCCAACCGCCCCCTCCGAAGATTTGCGGTTCCCCCGGAGGCCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCGTACAAGGAGCATGGTGTGTCAAAACAAGTGGCCAGATATAAGATTATCTTTGTTCATGGGTTCGGTTCTTCCAGACATGATGCAGTGATTGCGACCAAACCTTCACCAGAAGTTATTGAAGAGTTAGGGGTACACATTGTGTCTTTTGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGTTCCAAATTTTATGTAGTTGGATATTCTATGGGTGGACAGGTTATCTGGAGCTGTCTTCAGTACATCCCTCATAGGCTAGCTGGAGCTACACTGTATGCTCCTGTTGTCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCGACAGATGCCTACTATAAACAACCCCCCGGGGACCAATGGGCACTTCGAGTTGCTCACCATATACCATGGCTAACATACTGGTGGAACACTCAGAAATTATTTCCTTCATCAAGCGTTATAGTTGGTAAACCAGAGATTTTCTCCAGTCAGGACTTGGAAATACTTGGGAATATGCTGGCTTCTGGTATACAAACGAACAGGCCACACGGAAAGCAGCAAGGAGTATACGAGTCGAACCATCGTGACCTGATGATTGGATTTGGGAGTTGGGAGTTTGACCCTGTAGAAATCAAGAATCCATTTCCCAACAATGAAGGGTCCGTCCATCTGTGGCAGGGTAATGAAGATAGACTTGTCCCAGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTGACTACCATGAGTTAACAGGTGCTGGGCACTTGTTTGCATATAACGATGGTATAAATGAAGCCATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

38.84

Weight (kDa)

6.87

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 63 - 175 1.3e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 327 1.3e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 328 9.4e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 3 cut(s) 542, 730, 768
AciI CCGC 2 cut(s) 68, 87
AclWI GGATC 2 cut(s) 320, 326
AcoI YGGCCR 2 cut(s) 183, 518
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 440
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 3 cut(s) 155, 285, 461
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 3 cut(s) 272, 908, 914
AjnI CCWGG 2 cut(s) 307, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 8 cut(s) 116, 130, 376, 388, 450, 479, 485, 934
AluI AGCT 8 cut(s) 116, 130, 376, 388, 450, 479, 485, 934
AlwI GGATC 2 cut(s) 320, 326
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 5 cut(s) 57, 100, 183, 518, 743
ApeKI GCWGC 1 cut(s) 757
ApoI RAATTY 1 cut(s) 404
AspS9I GGNCC 3 cut(s) 101, 574, 861
AsuC2I CCSGG 3 cut(s) 96, 570, 571
AsuHPI GGTGA 3 cut(s) 249, 329, 590
AsuNHI GCTAGC 2 cut(s) 40, 475
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 2 cut(s) 574, 861
BaeGI GKGCMC 2 cut(s) 586, 975
BalI TGGCCA 2 cut(s) 185, 520
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 769
BccI CCATC 5 cut(s) 116, 131, 789, 876, 986
BciT130I CCWGG 2 cut(s) 309, 522
BcnI CCSGG 3 cut(s) 96, 570, 571
BfaI CTAG 3 cut(s) 41, 120, 476
BfmI CTRYAG 1 cut(s) 825
BisI GCNGC 1 cut(s) 758
BlsI GCNGC 1 cut(s) 759
Bme1390I CCNGG 5 cut(s) 96, 309, 522, 570, 571
Bme18I GGWCC 2 cut(s) 574, 861
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 101, 574, 861
BmiI GGNNCC 4 cut(s) 91, 400, 575, 862
BmrFI CCNGG 5 cut(s) 96, 309, 522, 570, 571
BmrI ACTGGG 2 cut(s) 896, 1038
BmsI GCATC 2 cut(s) 223, 538
BmtI GCTAGC 2 cut(s) 44, 479
BmuI ACTGGG 2 cut(s) 896, 1038
BpiI GAAGAC 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 3 cut(s) 466, 501, 670
Bpu10I CCTNAGC 1 cut(s) 336
BpuMI CCSGG 3 cut(s) 96, 570, 571
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 6 cut(s) 94, 568, 569, 570, 608, 938
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 5 cut(s) 518, 626, 687, 902, 1033
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 2 cut(s) 309, 522
BseDI CCNNGG 6 cut(s) 94, 568, 569, 570, 608, 938
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BseSI GKGCMC 2 cut(s) 586, 975
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 968
BshFI GGCC 5 cut(s) 59, 102, 185, 520, 745
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 2 cut(s) 96, 570
BslFI GGGAC 2 cut(s) 587, 884
BslI CCNNNNNNNGG 1 cut(s) 314
BsmFI GGGAC 2 cut(s) 587, 884
BsnI GGCC 5 cut(s) 59, 102, 185, 520, 745
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 586, 975
Bsp143I GATC 2 cut(s) 325, 331
Bsp19I CCATGG 2 cut(s) 608, 938
BspACI CCGC 2 cut(s) 68, 87
BspANI GGCC 5 cut(s) 59, 102, 185, 520, 745
BspCNI CTCAG 1 cut(s) 646
BspLI GGNNCC 4 cut(s) 91, 400, 575, 862
BspOI GCTAGC 2 cut(s) 44, 479
BspPI GGATC 2 cut(s) 320, 326
BspQI GCTCTTC 1 cut(s) 366
BsrI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BssECI CCNNGG 6 cut(s) 94, 568, 569, 570, 608, 938
BssMI GATC 2 cut(s) 325, 331
BssNAI GTATAC 2 cut(s) 731, 769
BssT1I CCWWGG 2 cut(s) 608, 938
Bst1107I GTATAC 2 cut(s) 731, 769
Bst2UI CCWGG 2 cut(s) 309, 522
Bst4CI ACNGT 2 cut(s) 346, 492
Bst6I CTCTTC 4 cut(s) 4, 267, 344, 366
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 4 cut(s) 42, 378, 477, 720
BstDEI CTNAG 3 cut(s) 131, 336, 633
BstDSI CCRYGG 2 cut(s) 608, 938
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 2 cut(s) 328, 334
BstMBI GATC 2 cut(s) 325, 331
BstMWI GCNNNNNNNGC 2 cut(s) 56, 242
BstNI CCWGG 2 cut(s) 309, 522
BstSCI CCNGG 5 cut(s) 94, 307, 520, 568, 569
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 2 cut(s) 586, 975
BstV1I GCAGC 1 cut(s) 769
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BstZ17I GTATAC 2 cut(s) 731, 769
BsuRI GGCC 5 cut(s) 59, 102, 185, 520, 745
BtgI CCRYGG 2 cut(s) 608, 938
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 351, 488
Cac8I GCNNGC 4 cut(s) 42, 378, 477, 720
CaiI CAGNNNCTG 1 cut(s) 968
Cfr13I GGNCC 3 cut(s) 101, 574, 861
Cfr9I CCCGGG 1 cut(s) 569
Csp6I GTAC 3 cut(s) 154, 284, 460
CspCI CAANNNNNGTGG 2 cut(s) 162, 197
CviAII CATG 8 cut(s) 45, 164, 209, 230, 609, 939, 953, 1009
CviQI GTAC 3 cut(s) 154, 284, 460
DdeI CTNAG 3 cut(s) 131, 336, 633
DpnI GATC 2 cut(s) 327, 333
DpnII GATC 2 cut(s) 325, 331
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 2 cut(s) 183, 518
Eam1104I CTCTTC 4 cut(s) 4, 267, 344, 366
EarI CTCTTC 4 cut(s) 4, 267, 344, 366
Eco130I CCWWGG 2 cut(s) 608, 938
Eco47I GGWCC 2 cut(s) 574, 861
Eco57I CTGAAG 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 307, 520
EcoT14I CCWWGG 2 cut(s) 608, 938
ErhI CCWWGG 2 cut(s) 608, 938
FaeI CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 879, 911
FaqI GGGAC 2 cut(s) 587, 884
FatI CATG 8 cut(s) 44, 163, 208, 229, 608, 938, 952, 1008
FblI GTMKAC 3 cut(s) 542, 730, 768
Fnu4HI GCNGC 1 cut(s) 758
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 1 cut(s) 758
FspBI CTAG 3 cut(s) 41, 120, 476
GluI GCNGC 1 cut(s) 758
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 3 cut(s) 466, 501, 670
HaeIII GGCC 5 cut(s) 59, 102, 185, 520, 745
HapII CCGG 2 cut(s) 96, 570
Hin1II CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
HincII GTYRAC 3 cut(s) 508, 543, 960
HindII GTYRAC 3 cut(s) 508, 543, 960
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 4 cut(s) 12, 393, 773, 838
HpaI GTTAAC 1 cut(s) 960
HpaII CCGG 2 cut(s) 96, 570
HphI GGTGA 3 cut(s) 249, 329, 590
Hpy166II GTNNAC 8 cut(s) 286, 434, 508, 543, 671, 731, 769, 960
Hpy188I TCNGA 3 cut(s) 78, 392, 636
Hpy188III TCNNGA 6 cut(s) 225, 329, 445, 692, 785, 835
Hpy8I GTNNAC 8 cut(s) 286, 434, 508, 543, 671, 731, 769, 960
HpyAV CCTTC 3 cut(s) 264, 657, 851
HpyCH4III ACNGT 2 cut(s) 346, 492
HpyCH4V TGCA 2 cut(s) 236, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 242
HpyF3I CTNAG 3 cut(s) 131, 336, 633
Hsp92II CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
KspAI GTTAAC 1 cut(s) 960
Kzo9I GATC 2 cut(s) 325, 331
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 5 cut(s) 121, 160, 447, 482, 502
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 2 cut(s) 223, 538
MaeI CTAG 3 cut(s) 41, 120, 476
MaeIII GTNAC 1 cut(s) 785
MalI GATC 2 cut(s) 327, 333
MboI GATC 2 cut(s) 325, 331
MboII GAAGA 9 cut(s) 21, 91, 153, 213, 284, 361, 383, 446, 899
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 2 cut(s) 586, 975
MlsI TGGCCA 2 cut(s) 185, 520
MluCI AATT 2 cut(s) 404, 638
MluNI TGGCCA 2 cut(s) 185, 520
MlyI GAGTC 2 cut(s) 21, 782
MmeI TCCRAC 2 cut(s) 13, 397
MnlI CCTC 3 cut(s) 84, 92, 477
Mox20I TGGCCA 2 cut(s) 185, 520
MscI TGGCCA 2 cut(s) 185, 520
MseI TTAA 2 cut(s) 959, 1013
MslI CAYNNNNRTG 1 cut(s) 622
Msp20I TGGCCA 2 cut(s) 185, 520
MspI CCGG 2 cut(s) 96, 570
MspR9I CCNGG 5 cut(s) 96, 309, 522, 570, 571
MvaI CCWGG 2 cut(s) 309, 522
MwoI GCNNNNNNNGC 2 cut(s) 56, 242
NciI CCSGG 3 cut(s) 96, 570, 571
NcoI CCATGG 2 cut(s) 608, 938
NdeII GATC 2 cut(s) 325, 331
NheI GCTAGC 2 cut(s) 40, 475
NlaIII CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
NlaIV GGNNCC 4 cut(s) 91, 400, 575, 862
NmuCI GTSAC 1 cut(s) 785
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 393, 838
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 1 cut(s) 759
PleI GAGTC 2 cut(s) 20, 781
PpsI GAGTC 2 cut(s) 20, 781
Psp6I CCWGG 2 cut(s) 307, 520
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 2 cut(s) 307, 520
PspN4I GGNNCC 4 cut(s) 91, 400, 575, 862
PspPI GGNCC 3 cut(s) 101, 574, 861
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 3 cut(s) 155, 285, 461
RsaNI GTAC 3 cut(s) 154, 284, 460
RseI CAYNNNNRTG 1 cut(s) 622
SalI GTCGAC 1 cut(s) 541
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 2 cut(s) 959, 1013
SatI GCNGC 1 cut(s) 758
Sau3AI GATC 2 cut(s) 325, 331
Sau96I GGNCC 3 cut(s) 101, 574, 861
SchI GAGTC 2 cut(s) 21, 782
ScrFI CCNGG 5 cut(s) 96, 309, 522, 570, 571
SduI GDGCHC 2 cut(s) 586, 975
SfaNI GCATC 2 cut(s) 223, 538
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 2 cut(s) 574, 861
SmaI CCCGGG 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 622
Sse9I AATT 2 cut(s) 404, 638
SsiI CCGC 2 cut(s) 68, 87
SspMI CTAG 3 cut(s) 41, 120, 476
StyD4I CCNGG 5 cut(s) 94, 307, 520, 568, 569
StyI CCWWGG 2 cut(s) 608, 938
TaaI ACNGT 2 cut(s) 346, 492
TaqI TCGA 3 cut(s) 542, 589, 776
TasI AATT 2 cut(s) 404, 638
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 2 cut(s) 393, 838
Tru1I TTAA 2 cut(s) 959, 1013
Tru9I TTAA 2 cut(s) 959, 1013
TscAI CASTG 3 cut(s) 243, 351, 495
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 1 cut(s) 757
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 5 cut(s) 197, 639, 870, 900, 1017
TspGWI ACGGA 2 cut(s) 765, 853
TspMI CCCGGG 1 cut(s) 569
TspRI CASTG 3 cut(s) 243, 351, 495
Van91I CCANNNNNTGG 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 574, 861
XapI RAATTY 1 cut(s) 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmaI CCCGGG 1 cut(s) 569
XmiI GTMKAC 3 cut(s) 542, 730, 768
XspI CTAG 3 cut(s) 41, 120, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.