pycom07g04990

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000873
Physical Location & Seq
Reverse (-)
4280649 .. 4286109
5461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04990.5

Sequence Viewer

Length: 1497 bp
ATGTTAAACTGTAAAATATCAAAAACCCTAGCGTCACATCCACTCGGGCTCTCGGGCTGGCAATTCTTCACTGGCCCGAAGCTCGGGCCTCCACCTCCACTCAGGCTCCTTCACGCTGGAGCTAGTCCACAGAGCCTTATGCCCCTTTTCTTTGGTCTGTCCCCCGAGCATCCACCTACGGTGGAGTTGCTCTTATTGAACCTTGTTTGGTCAAGTCTGATTAAAGCACTCCAACCAACCCAACAAATACGAGACAGGTGGCACCAAAGCACCATGGAAGCATTCTCCTCTTTTATTTCTTGTTTTCTTTTTAATCAACTCATTTTATTGTATCTTTGTGTTTTTGTTCCTCACGTTTCAAACTTCAAACATCTTCGATCTTTCTTTTCTCTCACAAATCATCATCTCCTTTCTCAAAATGTGAGCAAAGTTTCGTACTTTCCATCTTCTCATAACATGTTTAAGAGGATCACATTCATCTTGTTGGTTGGATTGCTGGCGTGGGCATATCAGGCCGCCCGACCACCACCTCCGAACATCTGTGGTTCGCCTGGCGGTCCACCCGTCACAGCGCCTAGAATAAGGCTAAGGGATGGAAGATTTTTGGCCTACAAGGAACATGGTGTTCCAAAACAAGTGGCCAAGTACAAGATTATCTTTGTTCATCCCTTCTTTAGTTCCCGACATGATCCAATCATTGCAACAGCCGTCTCTCCGGACGTTATTGAAGATTTAGGTGTGTACATTGTGTCTTTCGACAGACCAGGGTACGGTGAAAGTGATCCGGATCCTAAGCGGACAACGAAGAGCTTGGCGTTAGATATAGAGGAGCTTGGTGATCAGCTGGAACTCGGATCCAAATTTTATGTGATTGGATACTCAATGGGAGGACAGGTCATCTGGAAGTGTCTTCGGTACATCCCTCATAGGCTAGCTGGAGCAGCACTGATCGCTCCGTCGATCAACTACTGGTGGCCAGGACTTCCTGCCAACCTGTCATCTTATGCCTATAAGAAGCAAGCTCCACAGGATCAATGGGCGCTTCGAGCTGCCCACTATATACCTTGGTTAACCTACTGGTGGAACACTCAGAATTTCTTTCCTAAACACCGCGTTTTATCCGGGAAAATAGAGAATATGTTGTCCCGCCAAGATTGGGAAAACATTAGGAATGCAAAGGTTTCTGGAAGAGAAAAGCACAAGGAACATGCCACGCAGCAAGGAGAAGCTGAGTCCATCTTTCGCGACATGGTAGTTGGATTTGGGAGCTGGGAATTTGATCCTACGGATCTGCAGAACCCTTTTGTCAACAACGAAGGCTCGGTCCATCTATGGCAGGGTGACGATGATAAGTTTGTCTCAGTTGAGGTGCAACGCTATATTGCCGAAAAGCTTCCATGGATTCACTACCATGAGTTACCAGGTGGTGGACATTTGTTGCCAGCTGCAGATGGTATGAGTGAAATCATTTTAAAGGCACTTCTAATTAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

499

Amino Acids

56.48

Weight (kDa)

8.96

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AccB7I CCANNNNNTGG 1 cut(s) 1427
AccII CGCG 2 cut(s) 1113, 1245
AccIII TCCGGA 2 cut(s) 715, 784
AciI CCGC 5 cut(s) 516, 555, 796, 1111, 1147
AcoI YGGCCR 2 cut(s) 639, 974
AcsI RAATTY 3 cut(s) 860, 1093, 1274
AfaI GTAC 5 cut(s) 437, 647, 743, 770, 917
AfiI CCNNNNNNNGG 5 cut(s) 83, 770, 1080, 1156, 1427
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 4 cut(s) 199, 360, 367, 728
AjnI CCWGG 4 cut(s) 550, 763, 976, 1420
Alw26I GTCTC 3 cut(s) 246, 715, 1363
Ama87I CYCGRG 4 cut(s) 44, 52, 83, 164
Aor13HI TCCGGA 2 cut(s) 715, 784
AoxI GGCC 6 cut(s) 73, 86, 513, 606, 639, 974
ApeKI GCWGC 4 cut(s) 941, 1049, 1216, 1445
ApoI RAATTY 3 cut(s) 860, 1093, 1274
ArsI GACNNNNNNTTYG 2 cut(s) 1308, 1340
Asp700I GAANNNNTTC 1 cut(s) 1392
AspLEI GCGC 2 cut(s) 574, 1042
AspS9I GGNCC 4 cut(s) 74, 86, 557, 1324
AsuC2I CCSGG 1 cut(s) 1123
AsuHPI GGTGA 3 cut(s) 785, 848, 1352
AsuNHI GCTAGC 1 cut(s) 931
AvaI CYCGRG 4 cut(s) 44, 52, 83, 164
AvaII GGWCC 2 cut(s) 557, 1324
BalI TGGCCA 2 cut(s) 641, 976
BamHI GGATCC 2 cut(s) 787, 854
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 902
BbvI GCAGC 4 cut(s) 953, 1036, 1228, 1432
BccI CCATC 5 cut(s) 451, 587, 1244, 1335, 1445
BceAI ACGGC 1 cut(s) 692
BciT130I CCWGG 4 cut(s) 552, 765, 978, 1422
BciVI GTATCC 1 cut(s) 869
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 1 cut(s) 1123
BcoDI GTCTC 3 cut(s) 246, 715, 1363
BfaI CTAG 4 cut(s) 29, 123, 576, 932
BfmI CTRYAG 2 cut(s) 1292, 1446
BfoI RGCGCY 2 cut(s) 575, 1043
BfuI GTATCC 1 cut(s) 869
BisI GCNGC 5 cut(s) 516, 942, 1050, 1217, 1446
BlsI GCNGC 5 cut(s) 517, 943, 1051, 1218, 1447
Bme1390I CCNGG 5 cut(s) 552, 765, 978, 1123, 1422
Bme18I GGWCC 2 cut(s) 557, 1324
BmeT110I CYCGRG 4 cut(s) 44, 52, 83, 164
BmgT120I GGNCC 4 cut(s) 74, 86, 557, 1324
BmiI GGNNCC 4 cut(s) 107, 263, 789, 856
BmrFI CCNGG 5 cut(s) 552, 765, 978, 1123, 1422
BmsI GCATC 1 cut(s) 178
BmtI GCTAGC 1 cut(s) 935
BpiI GAAGAC 1 cut(s) 902
BpmI CTGGAG 2 cut(s) 138, 957
Bpu10I CCTNAGC 2 cut(s) 587, 792
BpuMI CCSGG 1 cut(s) 1123
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 4 cut(s) 273, 764, 1064, 1397
BsaWI WCCGGW 2 cut(s) 715, 784
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 879, 909
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 770, 1080, 1156, 1427
Bse1I ACTGG 3 cut(s) 76, 974, 1082
Bse3DI GCAATG 1 cut(s) 696
Bse8I GATNNNNATC 1 cut(s) 786
BseAI TCCGGA 2 cut(s) 715, 784
BseBI CCWGG 4 cut(s) 552, 765, 978, 1422
BseDI CCNNGG 4 cut(s) 273, 764, 1064, 1397
BseGI GGATG 5 cut(s) 37, 169, 598, 664, 918
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 5 cut(s) 83, 770, 1080, 1156, 1427
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 4 cut(s) 115, 1103, 1221, 1374
BseNI ACTGG 3 cut(s) 76, 974, 1082
BseRI GAGGAG 2 cut(s) 277, 842
BseXI GCAGC 4 cut(s) 953, 1036, 1228, 1432
BseYI CCCAGC 1 cut(s) 1269
Bsh1236I CGCG 2 cut(s) 1113, 1245
BshFI GGCC 6 cut(s) 75, 88, 515, 608, 641, 976
BshNI GGYRCC 1 cut(s) 261
BsiHKCI CYCGRG 4 cut(s) 44, 52, 83, 164
BsiSI CCGG 3 cut(s) 716, 785, 1122
BslFI GGGAC 2 cut(s) 145, 1129
BslI CCNNNNNNNGG 5 cut(s) 83, 770, 1080, 1156, 1427
BsmAI GTCTC 3 cut(s) 246, 715, 1363
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 2 cut(s) 145, 1129
BsmI GAATGC 2 cut(s) 281, 1177
BsnI GGCC 6 cut(s) 75, 88, 515, 608, 641, 976
BsoBI CYCGRG 4 cut(s) 44, 52, 83, 164
Bsp1286I GDGCHC 1 cut(s) 51
Bsp13I TCCGGA 2 cut(s) 715, 784
Bsp1407I TGTACA 1 cut(s) 741
Bsp19I CCATGG 2 cut(s) 273, 1397
Bsp68I TCGCGA 1 cut(s) 1245
BspACI CCGC 5 cut(s) 516, 555, 796, 1111, 1147
BspANI GGCC 6 cut(s) 75, 88, 515, 608, 641, 976
BspCNI CTCAG 4 cut(s) 114, 1102, 1222, 1373
BspEI TCCGGA 2 cut(s) 715, 784
BspFNI CGCG 2 cut(s) 1113, 1245
BspLI GGNNCC 4 cut(s) 107, 263, 789, 856
BspMAI CTGCAG 2 cut(s) 1296, 1450
BspOI GCTAGC 1 cut(s) 935
BspQI GCTCTTC 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 696
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 3 cut(s) 76, 974, 1082
BssECI CCNNGG 4 cut(s) 273, 764, 1064, 1397
BssT1I CCWWGG 3 cut(s) 273, 1064, 1397
Bst2UI CCWGG 4 cut(s) 552, 765, 978, 1422
Bst4CI ACNGT 3 cut(s) 11, 181, 773
Bst6I CTCTTC 2 cut(s) 800, 1183
BstAUI TGTACA 1 cut(s) 741
BstC8I GCNNGC 5 cut(s) 59, 498, 933, 1020, 1443
BstDEI CTNAG 6 cut(s) 101, 587, 792, 1089, 1230, 1360
BstDSI CCRYGG 2 cut(s) 273, 1397
BstF5I GGATG 5 cut(s) 37, 169, 598, 664, 918
BstFNI CGCG 2 cut(s) 1113, 1245
BstH2I RGCGCY 2 cut(s) 575, 1043
BstHHI GCGC 2 cut(s) 574, 1042
BstMAI GTCTC 3 cut(s) 246, 715, 1363
BstMWI GCNNNNNNNGC 4 cut(s) 512, 941, 950, 1046
BstNI CCWGG 4 cut(s) 552, 765, 978, 1422
BstNSI RCATGY 2 cut(s) 460, 1211
BstSCI CCNGG 5 cut(s) 550, 763, 976, 1121, 1420
BstSFI CTRYAG 2 cut(s) 1292, 1446
BstUI CGCG 2 cut(s) 1113, 1245
BstV1I GCAGC 4 cut(s) 953, 1036, 1228, 1432
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 3 cut(s) 787, 854, 1288
BstYI RGATCY 3 cut(s) 787, 854, 1288
BsuI GTATCC 1 cut(s) 869
BsuRI GGCC 6 cut(s) 75, 88, 515, 608, 641, 976
BtgI CCRYGG 2 cut(s) 273, 1397
BtsCI GGATG 5 cut(s) 37, 169, 598, 664, 918
BtsIMutI CAGTG 2 cut(s) 69, 944
BtuMI TCGCGA 1 cut(s) 1245
Cac8I GCNNGC 5 cut(s) 59, 498, 933, 1020, 1443
CfoI GCGC 2 cut(s) 574, 1042
Cfr13I GGNCC 4 cut(s) 74, 86, 557, 1324
CseI GACGC 1 cut(s) 21
CsiI ACCWGGT 1 cut(s) 1420
Csp6I GTAC 5 cut(s) 436, 646, 742, 769, 916
CspCI CAANNNNNGTGG 2 cut(s) 618, 653
CviAII CATG 8 cut(s) 274, 457, 620, 686, 1208, 1249, 1398, 1412
CviQI GTAC 5 cut(s) 436, 646, 742, 769, 916
DdeI CTNAG 6 cut(s) 101, 587, 792, 1089, 1230, 1360
DraI TTTAAA 1 cut(s) 1473
EaeI YGGCCR 2 cut(s) 639, 974
Eam1104I CTCTTC 2 cut(s) 800, 1183
EarI CTCTTC 2 cut(s) 800, 1183
Eco130I CCWWGG 3 cut(s) 273, 1064, 1397
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 557, 1324
Eco88I CYCGRG 4 cut(s) 44, 52, 83, 164
EcoRII CCWGG 4 cut(s) 550, 763, 976, 1420
EcoT14I CCWWGG 3 cut(s) 273, 1064, 1397
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 3 cut(s) 273, 1064, 1397
Esp3I CGTCTC 1 cut(s) 715
FaeI CATG 8 cut(s) 277, 460, 623, 689, 1211, 1252, 1401, 1415
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 2 cut(s) 145, 1129
FatI CATG 8 cut(s) 273, 456, 619, 685, 1207, 1248, 1397, 1411
FauI CCCGC 1 cut(s) 1154
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 5 cut(s) 516, 942, 1050, 1217, 1446
FokI GGATG 5 cut(s) 24, 156, 605, 651, 905
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 5 cut(s) 516, 942, 1050, 1217, 1446
FspBI CTAG 4 cut(s) 29, 123, 576, 932
GlaI GCGC 2 cut(s) 573, 1041
GluI GCNGC 5 cut(s) 516, 942, 1050, 1217, 1446
GsaI CCCAGC 1 cut(s) 1273
GsuI CTGGAG 2 cut(s) 138, 957
HaeII RGCGCY 2 cut(s) 575, 1043
HaeIII GGCC 6 cut(s) 75, 88, 515, 608, 641, 976
HapII CCGG 3 cut(s) 716, 785, 1122
HgaI GACGC 1 cut(s) 21
HhaI GCGC 2 cut(s) 574, 1042
Hin1II CATG 8 cut(s) 277, 460, 623, 689, 1211, 1252, 1401, 1415
Hin6I GCGC 2 cut(s) 572, 1040
HinP1I GCGC 2 cut(s) 572, 1040
HincII GTYRAC 2 cut(s) 1071, 1309
HindII GTYRAC 2 cut(s) 1071, 1309
HindIII AAGCTT 1 cut(s) 1391
HinfI GANTC 2 cut(s) 1232, 1402
HpaI GTTAAC 1 cut(s) 1071
HpaII CCGG 3 cut(s) 716, 785, 1122
HphI GGTGA 3 cut(s) 785, 848, 1352
Hpy166II GTNNAC 6 cut(s) 128, 560, 742, 1071, 1309, 1430
Hpy188I TCNGA 4 cut(s) 219, 534, 854, 1092
Hpy188III TCNNGA 6 cut(s) 681, 716, 785, 901, 1185, 1244
Hpy8I GTNNAC 6 cut(s) 128, 560, 742, 1071, 1309, 1430
Hpy99I CGWCG 1 cut(s) 961
HpyAV CCTTC 3 cut(s) 119, 679, 1310
HpyCH4III ACNGT 3 cut(s) 11, 181, 773
HpyCH4IV ACGT 2 cut(s) 354, 720
HpyCH4V TGCA 5 cut(s) 701, 1175, 1294, 1372, 1448
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 941, 950, 1046
HpyF3I CTNAG 6 cut(s) 101, 587, 792, 1089, 1230, 1360
HpySE526I ACGT 2 cut(s) 354, 720
Hsp92II CATG 8 cut(s) 277, 460, 623, 689, 1211, 1252, 1401, 1415
HspAI GCGC 2 cut(s) 572, 1040
Kpn2I TCCGGA 2 cut(s) 715, 784
Ksp22I TGATCA 1 cut(s) 838
KspAI GTTAAC 1 cut(s) 1071
LguI GCTCTTC 1 cut(s) 800
LmnI GCTCC 7 cut(s) 111, 119, 829, 938, 958, 1027, 1266
Lsp1109I GCAGC 4 cut(s) 953, 1036, 1228, 1432
LweI GCATC 1 cut(s) 178
MabI ACCWGGT 1 cut(s) 1420
MaeI CTAG 4 cut(s) 29, 123, 576, 932
MaeII ACGT 2 cut(s) 354, 720
MaeIII GTNAC 4 cut(s) 33, 565, 1340, 1416
MboII GAAGA 8 cut(s) 58, 365, 438, 609, 740, 817, 902, 1200
MflI RGATCY 3 cut(s) 787, 854, 1288
MhlI GDGCHC 1 cut(s) 51
MlsI TGGCCA 2 cut(s) 641, 976
MluCI AATT 5 cut(s) 62, 860, 1093, 1274, 1485
MluNI TGGCCA 2 cut(s) 641, 976
MlyI GAGTC 1 cut(s) 1241
MmeI TCCRAC 3 cut(s) 256, 469, 1237
Mox20I TGGCCA 2 cut(s) 641, 976
MroI TCCGGA 2 cut(s) 715, 784
MroXI GAANNNNTTC 1 cut(s) 1392
MscI TGGCCA 2 cut(s) 641, 976
MseI TTAA 7 cut(s) 5, 222, 312, 462, 1070, 1472, 1488
MslI CAYNNNNRTG 1 cut(s) 1410
Msp20I TGGCCA 2 cut(s) 641, 976
MspA1I CMGCKG 2 cut(s) 844, 1445
MspI CCGG 3 cut(s) 716, 785, 1122
MspR9I CCNGG 5 cut(s) 552, 765, 978, 1123, 1422
Mva1269I GAATGC 2 cut(s) 281, 1177
MvaI CCWGG 4 cut(s) 552, 765, 978, 1422
MvnI CGCG 2 cut(s) 1113, 1245
MwoI GCNNNNNNNGC 4 cut(s) 512, 941, 950, 1046
NciI CCSGG 1 cut(s) 1123
NcoI CCATGG 2 cut(s) 273, 1397
NheI GCTAGC 1 cut(s) 931
NlaIII CATG 8 cut(s) 277, 460, 623, 689, 1211, 1252, 1401, 1415
NlaIV GGNNCC 4 cut(s) 107, 263, 789, 856
NmuCI GTSAC 3 cut(s) 33, 565, 1340
NruI TCGCGA 1 cut(s) 1245
NspI RCATGY 2 cut(s) 460, 1211
PciI ACATGT 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 800
PctI GAATGC 2 cut(s) 281, 1177
PdmI GAANNNNTTC 1 cut(s) 1392
PfeI GAWTC 1 cut(s) 1402
PflMI CCANNNNNTGG 1 cut(s) 1427
PfoI TCCNGGA 1 cut(s) 1121
PkrI GCNGC 5 cut(s) 517, 943, 1051, 1218, 1447
PleI GAGTC 1 cut(s) 1240
PpsI GAGTC 1 cut(s) 1240
PscI ACATGT 1 cut(s) 456
Psp6I CCWGG 4 cut(s) 550, 763, 976, 1420
PspFI CCCAGC 1 cut(s) 1269
PspGI CCWGG 4 cut(s) 550, 763, 976, 1420
PspN4I GGNNCC 4 cut(s) 107, 263, 789, 856
PspPI GGNCC 4 cut(s) 74, 86, 557, 1324
PstI CTGCAG 2 cut(s) 1296, 1450
PsuI RGATCY 3 cut(s) 787, 854, 1288
PvuII CAGCTG 2 cut(s) 844, 1445
RruI TCGCGA 1 cut(s) 1245
RsaI GTAC 5 cut(s) 437, 647, 743, 770, 917
RsaNI GTAC 5 cut(s) 436, 646, 742, 769, 916
RseI CAYNNNNRTG 1 cut(s) 1410
SapI GCTCTTC 1 cut(s) 800
SaqAI TTAA 7 cut(s) 5, 222, 312, 462, 1070, 1472, 1488
SatI GCNGC 5 cut(s) 516, 942, 1050, 1217, 1446
Sau96I GGNCC 4 cut(s) 74, 86, 557, 1324
SchI GAGTC 1 cut(s) 1241
ScrFI CCNGG 5 cut(s) 552, 765, 978, 1123, 1422
SduI GDGCHC 1 cut(s) 51
SexAI ACCWGGT 1 cut(s) 1420
SfaNI GCATC 1 cut(s) 178
SfcI CTRYAG 2 cut(s) 1292, 1446
SinI GGWCC 2 cut(s) 557, 1324
SmiMI CAYNNNNRTG 1 cut(s) 1410
Sse9I AATT 5 cut(s) 62, 860, 1093, 1274, 1485
SsiI CCGC 5 cut(s) 516, 555, 796, 1111, 1147
SspMI CTAG 4 cut(s) 29, 123, 576, 932
StyD4I CCNGG 5 cut(s) 550, 763, 976, 1121, 1420
StyI CCWWGG 3 cut(s) 273, 1064, 1397
TaaI ACNGT 3 cut(s) 11, 181, 773
TaiI ACGT 2 cut(s) 357, 723
TaqI TCGA 4 cut(s) 376, 756, 959, 1045
TaqII GACCGA 1 cut(s) 1312
TasI AATT 5 cut(s) 62, 860, 1093, 1274, 1485
TatI WGTACW 2 cut(s) 645, 741
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 1 cut(s) 1402
Tru1I TTAA 7 cut(s) 5, 222, 312, 462, 1070, 1472, 1488
Tru9I TTAA 7 cut(s) 5, 222, 312, 462, 1070, 1472, 1488
TscAI CASTG 2 cut(s) 76, 951
TseFI GTSAC 3 cut(s) 33, 565, 1340
TseI GCWGC 4 cut(s) 941, 1049, 1216, 1445
Tsp45I GTSAC 3 cut(s) 33, 565, 1340
TspDTI ATGAA 2 cut(s) 466, 653
TspGWI ACGGA 2 cut(s) 945, 1301
TspRI CASTG 2 cut(s) 76, 951
Van91I CCANNNNNTGG 1 cut(s) 1427
VpaK11BI GGWCC 2 cut(s) 557, 1324
XapI RAATTY 3 cut(s) 860, 1093, 1274
XceI RCATGY 2 cut(s) 460, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 1032
XmnI GAANNNNTTC 1 cut(s) 1392
XspI CTAG 4 cut(s) 29, 123, 576, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.