Rw0G002590

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00079
OG0000873
Physical Location & Seq
Forward (+)
52803 .. 56881
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002590.1

Sequence Viewer

Length: 1050 bp
ATGAAGATGATGTTTAGGAGAGTCGCATTTTTGTTGTTGGTTGGATTGCTGGCATGGGCTTATCAGGCCACCCAACCGCCCCCTCCGAAGATTTGCGGTTCCCCCGGGGGCCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCCTACAAGGAGCATGGTGTGTCAAAACAAGTGGCCAGATATAAGATTATCTTTGTTCATGGGTTCGGCTCTTCCAGACATGATGCAGTGATTGCGACCAAACCTTCAGCAGAATTTATCGAAGAGTTAGGGGTACACATTGTGTCTTTTGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGATCCAAATTTTATATAGTTGGATATTCTATGGGCGGACAGGTTATCTGGAGCTGTCTTCAGTACATCCCTCATAGGCTAGCTGGAGCTACACTGTATGCTCCTGTTGTCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCGACAGATGCCTACAATAAACAACCCCCTGGGGATCAATGGGCACTTCGAGTTGCTCACCATATACCATGGCTAACATACTGGTGGAACAGTCAGAAATTATTTCCTTCATCAAGCGTTATAGTTGGTAAACCAGAGATTTTCTCCAGTCAGGACTTGGAAATACTTGGGAAGATGCTGGCTTCTGGCATACAAACTAACAGGCCACACGGAAAGCAGCAAGGAGTATACGAGTCGATCCATCGTGACCTGATGATTGGATTTGGGAGTTGGGAGTTTGACCCTGTAGAAATCAACAATCCATTTCCCAACAATGAAGGGTCCGTCCATCTGTGGCAGGGTGATGAAGATAGACTTGTCCCAGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTGACTACCATGAGTTAACAGGTGCTGGGCACTTGTTTGCGTATAACGATGGTATAAATGAAGCCATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

349

Amino Acids

39.21

Weight (kDa)

6.87

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 66 - 185 6.7e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 69 - 330 2.8e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 69 - 331 4.3e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 323
AccI GTMKAC 2 cut(s) 551, 777
AciI CCGC 3 cut(s) 77, 96, 441
AclWI GGATC 6 cut(s) 329, 335, 402, 415, 591, 781
AcoI YGGCCR 2 cut(s) 192, 527
AcsI RAATTY 2 cut(s) 272, 413
AcuI CTGAAG 2 cut(s) 249, 449
AdeI CACNNNGTG 1 cut(s) 301
AfaI GTAC 2 cut(s) 294, 470
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 2 cut(s) 917, 923
AjnI CCWGG 3 cut(s) 316, 529, 577
AluBI AGCT 8 cut(s) 125, 139, 385, 397, 459, 488, 494, 943
AluI AGCT 8 cut(s) 125, 139, 385, 397, 459, 488, 494, 943
AlwI GGATC 6 cut(s) 329, 335, 402, 415, 591, 781
AlwNI CAGNNNCTG 1 cut(s) 977
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 6 cut(s) 66, 109, 159, 192, 527, 752
ApaI GGGCCC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 766
ApoI RAATTY 2 cut(s) 272, 413
AspS9I GGNCC 3 cut(s) 109, 110, 870
AsuC2I CCSGG 2 cut(s) 105, 106
AsuHPI GGTGA 3 cut(s) 338, 599, 902
AsuNHI GCTAGC 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 870
BaeGI GKGCMC 3 cut(s) 113, 595, 984
BalI TGGCCA 2 cut(s) 194, 529
BamHI GGATCC 1 cut(s) 407
BanII GRGCYC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 455
BbvI GCAGC 1 cut(s) 778
BccI CCATC 5 cut(s) 125, 140, 798, 885, 995
BciT130I CCWGG 3 cut(s) 318, 531, 579
BcnI CCSGG 2 cut(s) 105, 106
BfaI CTAG 2 cut(s) 129, 485
BfmI CTRYAG 1 cut(s) 834
BisI GCNGC 1 cut(s) 767
BlsI GCNGC 1 cut(s) 768
Bme1390I CCNGG 5 cut(s) 105, 106, 318, 531, 579
Bme18I GGWCC 1 cut(s) 870
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 109, 110, 870
BmiI GGNNCC 5 cut(s) 100, 110, 111, 409, 871
BmrFI CCNGG 5 cut(s) 105, 106, 318, 531, 579
BmrI ACTGGG 2 cut(s) 905, 1047
BmsI GCATC 3 cut(s) 232, 547, 714
BmtI GCTAGC 1 cut(s) 488
BmuI ACTGGG 2 cut(s) 905, 1047
BpiI GAAGAC 1 cut(s) 455
BplI GAGNNNNNCTC 2 cut(s) 677, 709
BpmI CTGGAG 3 cut(s) 475, 510, 679
Bpu10I CCTNAGC 1 cut(s) 345
BpuMI CCSGG 2 cut(s) 105, 106
BsaBI GATNNNNATC 2 cut(s) 339, 406
BsaJI CCNNGG 7 cut(s) 103, 104, 105, 577, 578, 617, 947
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 5 cut(s) 527, 635, 696, 911, 1042
Bse8I GATNNNNATC 2 cut(s) 339, 406
BseBI CCWGG 3 cut(s) 318, 531, 579
BseDI CCNNGG 7 cut(s) 103, 104, 105, 577, 578, 617, 947
BseGI GGATG 1 cut(s) 471
BseJI GATNNNNATC 2 cut(s) 339, 406
BseLI CCNNNNNNNGG 1 cut(s) 323
BseNI ACTGG 5 cut(s) 527, 635, 696, 911, 1042
BseSI GKGCMC 3 cut(s) 113, 595, 984
BseXI GCAGC 1 cut(s) 778
BseYI CCCAGC 1 cut(s) 977
BshFI GGCC 6 cut(s) 68, 111, 161, 194, 529, 754
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 893
BslI CCNNNNNNNGG 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 893
BsnI GGCC 6 cut(s) 68, 111, 161, 194, 529, 754
BsoBI CYCGRG 1 cut(s) 104
Bsp120I GGGCCC 1 cut(s) 109
Bsp1286I GDGCHC 3 cut(s) 113, 595, 984
Bsp143I GATC 5 cut(s) 334, 340, 407, 583, 786
Bsp19I CCATGG 2 cut(s) 617, 947
BspACI CCGC 3 cut(s) 77, 96, 441
BspANI GGCC 6 cut(s) 68, 111, 161, 194, 529, 754
BspLI GGNNCC 5 cut(s) 100, 110, 111, 409, 871
BspOI GCTAGC 1 cut(s) 488
BspPI GGATC 6 cut(s) 329, 335, 402, 415, 591, 781
BspQI GCTCTTC 2 cut(s) 235, 375
BsrI ACTGG 5 cut(s) 527, 635, 696, 911, 1042
BssECI CCNNGG 7 cut(s) 103, 104, 105, 577, 578, 617, 947
BssMI GATC 5 cut(s) 334, 340, 407, 583, 786
BssNAI GTATAC 1 cut(s) 778
BssT1I CCWWGG 2 cut(s) 617, 947
Bst1107I GTATAC 1 cut(s) 778
Bst2UI CCWGG 3 cut(s) 318, 531, 579
Bst4CI ACNGT 3 cut(s) 355, 501, 641
Bst6I CTCTTC 4 cut(s) 235, 276, 353, 375
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 4 cut(s) 51, 387, 486, 729
BstDEI CTNAG 2 cut(s) 140, 345
BstDSI CCRYGG 2 cut(s) 617, 947
BstF5I GGATG 1 cut(s) 471
BstKTI GATC 5 cut(s) 337, 343, 410, 586, 789
BstMBI GATC 5 cut(s) 334, 340, 407, 583, 786
BstMWI GCNNNNNNNGC 2 cut(s) 65, 251
BstNI CCWGG 3 cut(s) 318, 531, 579
BstSCI CCNGG 5 cut(s) 103, 104, 316, 529, 577
BstSFI CTRYAG 1 cut(s) 834
BstSLI GKGCMC 3 cut(s) 113, 595, 984
BstV1I GCAGC 1 cut(s) 778
BstV2I GAAGAC 1 cut(s) 455
BstX2I RGATCY 2 cut(s) 340, 407
BstYI RGATCY 2 cut(s) 340, 407
BstZ17I GTATAC 1 cut(s) 778
BsuRI GGCC 6 cut(s) 68, 111, 161, 194, 529, 754
BtgI CCRYGG 2 cut(s) 617, 947
BtsCI GGATG 1 cut(s) 471
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 252, 360, 497
Cac8I GCNNGC 4 cut(s) 51, 387, 486, 729
CaiI CAGNNNCTG 1 cut(s) 977
Cfr13I GGNCC 3 cut(s) 109, 110, 870
Cfr9I CCCGGG 1 cut(s) 104
Csp6I GTAC 2 cut(s) 293, 469
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 8 cut(s) 54, 173, 218, 239, 618, 948, 962, 1018
CviQI GTAC 2 cut(s) 293, 469
DdeI CTNAG 2 cut(s) 140, 345
DpnI GATC 5 cut(s) 336, 342, 409, 585, 788
DpnII GATC 5 cut(s) 334, 340, 407, 583, 786
DraIII CACNNNGTG 1 cut(s) 301
EaeI YGGCCR 2 cut(s) 192, 527
Eam1104I CTCTTC 4 cut(s) 235, 276, 353, 375
EarI CTCTTC 4 cut(s) 235, 276, 353, 375
EciI GGCGGA 1 cut(s) 456
Eco130I CCWWGG 2 cut(s) 617, 947
Eco24I GRGCYC 1 cut(s) 113
Eco47I GGWCC 1 cut(s) 870
Eco57I CTGAAG 2 cut(s) 249, 449
Eco88I CYCGRG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 3 cut(s) 316, 529, 577
EcoT14I CCWWGG 2 cut(s) 617, 947
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 2 cut(s) 617, 947
FaeI CATG 8 cut(s) 57, 176, 221, 242, 621, 951, 965, 1021
FalI AAGNNNNNCTT 4 cut(s) 194, 226, 888, 920
FaqI GGGAC 1 cut(s) 893
FatI CATG 8 cut(s) 53, 172, 217, 238, 617, 947, 961, 1017
FblI GTMKAC 2 cut(s) 551, 777
Fnu4HI GCNGC 1 cut(s) 767
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 767
FspBI CTAG 2 cut(s) 129, 485
GluI GCNGC 1 cut(s) 767
GsaI CCCAGC 1 cut(s) 981
GsuI CTGGAG 3 cut(s) 475, 510, 679
HaeIII GGCC 6 cut(s) 68, 111, 161, 194, 529, 754
HapII CCGG 1 cut(s) 105
Hin1II CATG 8 cut(s) 57, 176, 221, 242, 621, 951, 965, 1021
HincII GTYRAC 3 cut(s) 517, 552, 969
HindII GTYRAC 3 cut(s) 517, 552, 969
HindIII AAGCTT 1 cut(s) 941
HinfI GANTC 3 cut(s) 21, 402, 782
HpaI GTTAAC 1 cut(s) 969
HpaII CCGG 1 cut(s) 105
HphI GGTGA 3 cut(s) 338, 599, 902
Hpy166II GTNNAC 6 cut(s) 295, 517, 552, 680, 778, 969
Hpy188I TCNGA 4 cut(s) 87, 401, 407, 645
Hpy188III TCNNGA 5 cut(s) 234, 338, 454, 701, 794
Hpy8I GTNNAC 6 cut(s) 295, 517, 552, 680, 778, 969
HpyAV CCTTC 3 cut(s) 273, 666, 860
HpyCH4III ACNGT 3 cut(s) 355, 501, 641
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 251
HpyF3I CTNAG 2 cut(s) 140, 345
Hsp92II CATG 8 cut(s) 57, 176, 221, 242, 621, 951, 965, 1021
KspAI GTTAAC 1 cut(s) 969
Kzo9I GATC 5 cut(s) 334, 340, 407, 583, 786
LguI GCTCTTC 2 cut(s) 235, 375
LmnI GCTCC 5 cut(s) 130, 169, 456, 491, 511
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 3 cut(s) 232, 547, 714
MaeI CTAG 2 cut(s) 129, 485
MaeIII GTNAC 1 cut(s) 794
MalI GATC 5 cut(s) 336, 342, 409, 585, 788
MboI GATC 5 cut(s) 334, 340, 407, 583, 786
MflI RGATCY 2 cut(s) 340, 407
MhlI GDGCHC 3 cut(s) 113, 595, 984
MlsI TGGCCA 2 cut(s) 194, 529
MluCI AATT 3 cut(s) 272, 413, 647
MluNI TGGCCA 2 cut(s) 194, 529
MlyI GAGTC 2 cut(s) 30, 791
MmeI TCCRAC 2 cut(s) 22, 406
MnlI CCTC 2 cut(s) 93, 486
Mox20I TGGCCA 2 cut(s) 194, 529
MscI TGGCCA 2 cut(s) 194, 529
MseI TTAA 2 cut(s) 968, 1022
MslI CAYNNNNRTG 1 cut(s) 631
Msp20I TGGCCA 2 cut(s) 194, 529
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 5 cut(s) 105, 106, 318, 531, 579
MvaI CCWGG 3 cut(s) 318, 531, 579
MwoI GCNNNNNNNGC 2 cut(s) 65, 251
NciI CCSGG 2 cut(s) 105, 106
NcoI CCATGG 2 cut(s) 617, 947
NdeII GATC 5 cut(s) 334, 340, 407, 583, 786
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 8 cut(s) 57, 176, 221, 242, 621, 951, 965, 1021
NlaIV GGNNCC 5 cut(s) 100, 110, 111, 409, 871
NmuCI GTSAC 1 cut(s) 794
PasI CCCWGGG 1 cut(s) 578
PciSI GCTCTTC 2 cut(s) 235, 375
PfeI GAWTC 1 cut(s) 402
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 1 cut(s) 768
PleI GAGTC 2 cut(s) 29, 790
PpsI GAGTC 2 cut(s) 29, 790
Psp6I CCWGG 3 cut(s) 316, 529, 577
PspFI CCCAGC 1 cut(s) 977
PspGI CCWGG 3 cut(s) 316, 529, 577
PspN4I GGNNCC 5 cut(s) 100, 110, 111, 409, 871
PspOMI GGGCCC 1 cut(s) 109
PspPI GGNCC 3 cut(s) 109, 110, 870
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 2 cut(s) 340, 407
RsaI GTAC 2 cut(s) 294, 470
RsaNI GTAC 2 cut(s) 293, 469
RseI CAYNNNNRTG 1 cut(s) 631
SalI GTCGAC 1 cut(s) 550
SapI GCTCTTC 2 cut(s) 235, 375
SaqAI TTAA 2 cut(s) 968, 1022
SatI GCNGC 1 cut(s) 767
Sau3AI GATC 5 cut(s) 334, 340, 407, 583, 786
Sau96I GGNCC 3 cut(s) 109, 110, 870
SchI GAGTC 2 cut(s) 30, 791
ScrFI CCNGG 5 cut(s) 105, 106, 318, 531, 579
SduI GDGCHC 3 cut(s) 113, 595, 984
SfaNI GCATC 3 cut(s) 232, 547, 714
SfcI CTRYAG 1 cut(s) 834
SinI GGWCC 1 cut(s) 870
SmaI CCCGGG 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 631
Sse9I AATT 3 cut(s) 272, 413, 647
SsiI CCGC 3 cut(s) 77, 96, 441
SspMI CTAG 2 cut(s) 129, 485
StyD4I CCNGG 5 cut(s) 103, 104, 316, 529, 577
StyI CCWWGG 2 cut(s) 617, 947
TaaI ACNGT 3 cut(s) 355, 501, 641
TaqI TCGA 4 cut(s) 279, 551, 598, 785
TasI AATT 3 cut(s) 272, 413, 647
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 1 cut(s) 402
Tru1I TTAA 2 cut(s) 968, 1022
Tru9I TTAA 2 cut(s) 968, 1022
TscAI CASTG 3 cut(s) 252, 360, 504
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 1 cut(s) 766
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 6 cut(s) 17, 206, 648, 879, 909, 1026
TspGWI ACGGA 2 cut(s) 774, 862
TspMI CCCGGG 1 cut(s) 104
TspRI CASTG 3 cut(s) 252, 360, 504
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 870
XapI RAATTY 2 cut(s) 272, 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmaI CCCGGG 1 cut(s) 104
XmiI GTMKAC 2 cut(s) 551, 777
XspI CTAG 2 cut(s) 129, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.