Rorug01G0101600

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000873
Physical Location & Seq
Forward (+)
16899674 .. 16909808
10135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0101600.1

Sequence Viewer

Length: 1041 bp
ATGGCCGGATTCCGGTCACCGGAGTCCGCGTCCAAGGGAGAATTGGATGATGGGACAAAAATAGCAGTGAAAAGAATGGAGGCAGGTGTGATTAGCAACAAAGCCTTGGATGAATTCCAGTCCGAAATTGCAGTTCTTTCAAAGGTCCGACACCGTCATTTGGTATCACTGTTGGGTTATTCTGTTGCAGGAAATGAGAGAATGCTTGTGTATGAATATATGCCTCAAGGGGCTCTGAGCAGGCATCTTTTTCATTGGAAAACCTTTAAACTAGAACCTCTCTCTTGGATGAGGAGGCTAAATATTGCCTTGGACGTCGCTAGGGGGCTGGAGTATCTTCATAATCTTGCACACCAAAGCTTCATACACAGAGACCTCAAGTCATCAAACATCTTACTTGGTGATGATTTTAAAGCAAAAATATCAGATTTTGGATTGGTGAAACTAGCTCCTGATGGTGAAAAATCTGTTGTGACCAGGCTTGCTGGAACGTTTGGTTACTTAGCACCAGAGTATGCTGTGACAGGAAAAATTACAACCAAAGTAGATGTATTCAGTTTTGGGGTTGTGCTAATGGAGCTATTAACTGGAATGATGGCGCTGGATGATGATAGGCCTGAAGAAAGCCAGTACTTGGCTGCATGGTTCTGGCACATCAAATCAAATAAGGAGAAACTGTTGGCAGCCATTGACCCAACCCTTGATATAAAAGACATGAAAGAAGAAACATTTGAGAGCATCGCCACAATTGCGGAACTAGCTGGGCACTGCACTGCAAGGGAACCCAGCCAAAGACCAGATATGGGTCATGCTGTGAATGTATTGTCGACCCCACTGGTTGAAAAATGGAAACCATTTAATGATGAGGATGATGAATATTCTGGCATAGACTACAGCCTCCCGCTTAACCAGATGGTGAAAGGTTGGCAGGAAGCAGAAGGAAAGGATTCCAGTTACATGGACCTAGAAGACAGCAAGAGCAGTATACCAGCACGGCCAACTGGGTTTGCAGACTCTTTTACTTCGGCTGATGGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

38.67

Weight (kDa)

5.37

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 16 - 273 1e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 16 - 273 7.7e-41 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
AatII GACGTC 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 74
AccB7I CCANNNNNTGG 1 cut(s) 634
AccI GTMKAC 2 cut(s) 827, 985
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 27, 752, 902
AclI AACGTT 1 cut(s) 491
AcoI YGGCCR 2 cut(s) 3, 995
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 1 cut(s) 632
AfiI CCNNNNNNNGG 5 cut(s) 12, 19, 160, 634, 803
AgsI TTSAA 2 cut(s) 141, 842
AhdI GACNNNNNGTC 1 cut(s) 379
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 4 cut(s) 360, 449, 580, 761
AluI AGCT 4 cut(s) 360, 449, 580, 761
Alw26I GTCTC 1 cut(s) 366
AoxI GGCC 3 cut(s) 3, 614, 995
ApeKI GCWGC 2 cut(s) 638, 683
ApoI RAATTY 1 cut(s) 113
Asp700I GAANNNNTTC 1 cut(s) 946
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 2 cut(s) 145, 961
AsuHPI GGTGA 5 cut(s) 9, 413, 451, 470, 928
AvaII GGWCC 2 cut(s) 145, 961
BaeGI GKGCMC 1 cut(s) 768
BanII GRGCYC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 975
BbvI GCAGC 2 cut(s) 625, 695
BccI CCATC 5 cut(s) 44, 449, 589, 907, 1025
BceAI ACGGC 1 cut(s) 1010
BciT130I CCWGG 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 366
BfaI CTAG 5 cut(s) 272, 321, 446, 758, 965
BfmI CTRYAG 1 cut(s) 892
BfoI RGCGCY 1 cut(s) 602
BfuAI ACCTGC 1 cut(s) 74
BisI GCNGC 2 cut(s) 639, 684
BlsI GCNGC 2 cut(s) 640, 685
BmcAI AGTACT 1 cut(s) 632
Bme1390I CCNGG 1 cut(s) 478
Bme18I GGWCC 2 cut(s) 145, 961
BmeRI GACNNNNNGTC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 145, 961
BmiI GGNNCC 1 cut(s) 783
BmrFI CCNGG 1 cut(s) 478
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 2 cut(s) 253, 747
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 975
BpmI CTGGAG 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 210, 362
BsaHI GRCGYC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 366
BsaJI CCNNGG 3 cut(s) 33, 105, 309
BsaWI WCCGGW 2 cut(s) 12, 19
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 662, 692
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 19, 160, 634, 803
Bse1I ACTGG 6 cut(s) 118, 592, 628, 840, 951, 1006
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 3 cut(s) 33, 105, 309
BseGI GGATG 5 cut(s) 52, 115, 294, 610, 874
BseLI CCNNNNNNNGG 5 cut(s) 12, 19, 160, 634, 803
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 6 cut(s) 118, 592, 628, 840, 951, 1006
BseRI GAGGAG 1 cut(s) 307
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 2 cut(s) 625, 695
BseYI CCCAGC 2 cut(s) 761, 785
BsgI GTGCAG 1 cut(s) 754
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 3 cut(s) 5, 616, 997
BsiSI CCGG 3 cut(s) 6, 13, 20
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 5 cut(s) 12, 19, 160, 634, 803
BsmAI GTCTC 1 cut(s) 366
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 3 cut(s) 5, 616, 997
Bso31I GGTCTC 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 235, 768
BspACI CCGC 3 cut(s) 27, 752, 902
BspANI GGCC 3 cut(s) 5, 616, 997
BspCNI CTCAG 1 cut(s) 228
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 783
BspMI ACCTGC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 366
BsrI ACTGG 6 cut(s) 118, 592, 628, 840, 951, 1006
BssECI CCNNGG 3 cut(s) 33, 105, 309
BssNAI GTATAC 1 cut(s) 986
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 3 cut(s) 33, 105, 309
Bst1107I GTATAC 1 cut(s) 986
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 3 cut(s) 155, 171, 678
BstACI GRCGYC 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 242, 483
BstDEI CTNAG 2 cut(s) 236, 502
BstEII GGTNACC 1 cut(s) 15
BstF5I GGATG 5 cut(s) 52, 115, 294, 610, 874
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 1 cut(s) 601
BstMAI GTCTC 1 cut(s) 366
BstMWI GCNNNNNNNGC 3 cut(s) 577, 749, 758
BstNI CCWGG 1 cut(s) 478
BstPI GGTNACC 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 1 cut(s) 892
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 2 cut(s) 625, 695
BstV2I GAAGAC 1 cut(s) 975
BstXI CCANNNNNNTGG 1 cut(s) 958
BstZ17I GTATAC 1 cut(s) 986
BsuRI GGCC 3 cut(s) 5, 616, 997
BtgZI GCGATG 1 cut(s) 724
BtsCI GGATG 5 cut(s) 52, 115, 294, 610, 874
BtsI GCAGTG 3 cut(s) 72, 766, 771
BtsIMutI CAGTG 5 cut(s) 72, 167, 766, 771, 833
BveI ACCTGC 1 cut(s) 74
Cac8I GCNNGC 2 cut(s) 242, 483
CfoI GCGC 1 cut(s) 601
Cfr13I GGNCC 2 cut(s) 145, 961
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 631
CviAII CATG 4 cut(s) 642, 715, 809, 958
CviQI GTAC 1 cut(s) 631
DdeI CTNAG 2 cut(s) 236, 502
DraI TTTAAA 2 cut(s) 268, 412
DriI GACNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 2 cut(s) 3, 995
Eam1105I GACNNNNNGTC 1 cut(s) 379
Eco130I CCWWGG 3 cut(s) 33, 105, 309
Eco147I AGGCCT 1 cut(s) 616
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 366
Eco47I GGWCC 2 cut(s) 145, 961
Eco57I CTGAAG 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 15
EcoO65I GGTNACC 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 3 cut(s) 33, 105, 309
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 3 cut(s) 33, 105, 309
FaeI CATG 4 cut(s) 645, 718, 812, 961
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 641, 714, 808, 957
FauI CCCGC 1 cut(s) 909
FblI GTMKAC 2 cut(s) 827, 985
Fnu4HI GCNGC 2 cut(s) 639, 684
FokI GGATG 5 cut(s) 59, 122, 301, 617, 881
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 2 cut(s) 639, 684
FspBI CTAG 5 cut(s) 272, 321, 446, 758, 965
GlaI GCGC 1 cut(s) 600
GluI GCNGC 2 cut(s) 639, 684
GsaI CCCAGC 2 cut(s) 765, 789
GsuI CTGGAG 1 cut(s) 350
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 3 cut(s) 5, 616, 997
HapII CCGG 3 cut(s) 6, 13, 20
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 4 cut(s) 645, 718, 812, 961
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HincII GTYRAC 1 cut(s) 828
HindII GTYRAC 1 cut(s) 828
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 4 cut(s) 9, 23, 947, 1013
HpaII CCGG 3 cut(s) 6, 13, 20
HphI GGTGA 5 cut(s) 9, 413, 451, 470, 928
Hpy166II GTNNAC 2 cut(s) 828, 986
Hpy188I TCNGA 4 cut(s) 124, 149, 237, 427
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 2 cut(s) 828, 986
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 932
HpyCH4III ACNGT 3 cut(s) 155, 171, 678
HpyCH4IV ACGT 2 cut(s) 315, 491
HpyCH4V TGCA 7 cut(s) 131, 188, 350, 641, 771, 776, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 577, 749, 758
HpyF3I CTNAG 2 cut(s) 236, 502
HpySE526I ACGT 2 cut(s) 315, 491
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 4 cut(s) 645, 718, 812, 961
HspAI GCGC 1 cut(s) 599
LmnI GCTCC 2 cut(s) 454, 577
Lsp1109I GCAGC 2 cut(s) 625, 695
LweI GCATC 2 cut(s) 253, 747
MaeI CTAG 5 cut(s) 272, 321, 446, 758, 965
MaeII ACGT 2 cut(s) 315, 491
MaeIII GTNAC 5 cut(s) 15, 472, 497, 520, 953
MboII GAAGA 4 cut(s) 329, 632, 734, 980
MfeI CAATTG 1 cut(s) 747
MhlI GDGCHC 2 cut(s) 235, 768
MluCI AATT 5 cut(s) 41, 113, 126, 531, 747
MlyI GAGTC 2 cut(s) 32, 1007
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 8 cut(s) 73, 234, 285, 288, 288, 386, 859, 908
MroXI GAANNNNTTC 1 cut(s) 946
MseI TTAA 5 cut(s) 267, 411, 584, 858, 906
MspI CCGG 3 cut(s) 6, 13, 20
MspR9I CCNGG 1 cut(s) 478
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 207
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 577, 749, 758
NlaIII CATG 4 cut(s) 645, 718, 812, 961
NlaIV GGNNCC 1 cut(s) 783
NmuCI GTSAC 3 cut(s) 15, 472, 520
PaqCI CACCTGC 1 cut(s) 74
PceI AGGCCT 1 cut(s) 616
PctI GAATGC 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 946
PfeI GAWTC 2 cut(s) 9, 947
PflFI GACNNNGTC 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 634
PkrI GCNGC 2 cut(s) 640, 685
PleI GAGTC 2 cut(s) 31, 1007
PpsI GAGTC 2 cut(s) 31, 1007
Psp1406I AACGTT 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 476
PspEI GGTNACC 1 cut(s) 15
PspFI CCCAGC 2 cut(s) 761, 785
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 1 cut(s) 783
PspPI GGNCC 2 cut(s) 145, 961
PsyI GACNNNGTC 1 cut(s) 153
RsaI GTAC 1 cut(s) 632
RsaNI GTAC 1 cut(s) 631
SalI GTCGAC 1 cut(s) 826
SaqAI TTAA 5 cut(s) 267, 411, 584, 858, 906
SatI GCNGC 2 cut(s) 639, 684
Sau96I GGNCC 2 cut(s) 145, 961
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 2 cut(s) 32, 1007
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 2 cut(s) 235, 768
SfaNI GCATC 2 cut(s) 253, 747
SfcI CTRYAG 1 cut(s) 892
SinI GGWCC 2 cut(s) 145, 961
SmlI CTYRAG 2 cut(s) 225, 377
SmoI CTYRAG 2 cut(s) 225, 377
Sse9I AATT 5 cut(s) 41, 113, 126, 531, 747
SseBI AGGCCT 1 cut(s) 616
SsiI CCGC 3 cut(s) 27, 752, 902
SspI AATATT 2 cut(s) 304, 878
SspMI CTAG 5 cut(s) 272, 321, 446, 758, 965
StuI AGGCCT 1 cut(s) 616
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 3 cut(s) 33, 105, 309
TaaI ACNGT 3 cut(s) 155, 171, 678
TaiI ACGT 2 cut(s) 318, 494
TaqI TCGA 1 cut(s) 827
TasI AATT 5 cut(s) 41, 113, 126, 531, 747
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 2 cut(s) 9, 947
Tru1I TTAA 5 cut(s) 267, 411, 584, 858, 906
Tru9I TTAA 5 cut(s) 267, 411, 584, 858, 906
TscAI CASTG 5 cut(s) 72, 174, 773, 778, 840
TseFI GTSAC 3 cut(s) 15, 472, 520
TseI GCWGC 2 cut(s) 638, 683
Tsp45I GTSAC 3 cut(s) 15, 472, 520
TspDTI ATGAA 7 cut(s) 126, 228, 242, 329, 352, 731, 888
TspRI CASTG 5 cut(s) 72, 174, 773, 778, 840
Tth111I GACNNNGTC 1 cut(s) 153
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 145, 961
XapI RAATTY 1 cut(s) 113
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XmiI GTMKAC 2 cut(s) 827, 985
XmnI GAANNNNTTC 1 cut(s) 946
XspI CTAG 5 cut(s) 272, 321, 446, 758, 965
ZraI GACGTC 1 cut(s) 316
ZrmI AGTACT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.