RchiOBHm_Chr1g0332431

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000873
Physical Location & Seq
Reverse (-)
23596227 .. 23599875
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56133

Sequence Viewer

Length: 1041 bp
ATGTTTAGGAGAGTGGCACTTTTGTTGTTGGTTGGATTGCTGGCATGGGCTTATCAGGCCACCCAACCGCCCCCTCCGAAGATTTGCGGTTCCCCCGGGGGCCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCCTACAAGGAGCATGGTGTGTCGAAACAAGTGGCCAGATATAAGATTATCTTTGTTCATGGGTTCGGCTCTTCCAGACATGATGCAGTGATTGCGACCAAACCTTCAGCAGAATTTATCGAAGAGTTAGGGGTACACATTGTGTCTTTGGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGATCCAAATTTTATGTAGTTGGATATTCTATGGGTGGACAGGTTATCTGGAGCTGTCTTCAGTACATCCCTCATAGGCTAGCTGGAGCTACACTGTATGCTCCTGTTGTCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCGACAGATGCCTACTATAAACAACCCCCCGGGGATCAATGGGCACTTCGAGTTGCTCACCATATACCATGGCTAACATACTGGTGGAACACTCAGAAATTATTTCCTTCATCAAGCGTTATAGTTGGTAAACCAGAGATTTTCTCCAGTCAGGACTTGGAAATACGTAGGAAGATGCAGGCTTCTGGCATACAAACTAACAGGCCACATGGAAAGCAGCAAGGAGTATACGAGTCGAACCATCGTGACATGATGATTGGATTTGGGAGATGGGAGTTTGACCCTATAGAAATCAAGAACCCATTTCCCAACAATGAAGGGTCCGTCCATCTGTGGCAGGGTGATGAAGATAGGCTTGTCCCGGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTGACTACCATGAGTTACCAGGTGCTGGGCACTTGTTTGCATATAACGATGGTATAAATGAAGCCATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

39.07

Weight (kDa)

8.34

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 63 - 325 2e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 327 6e-18 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 328 4.7e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 314, 968
AccI GTMKAC 2 cut(s) 542, 768
AciI CCGC 2 cut(s) 68, 87
AclWI GGATC 5 cut(s) 320, 326, 393, 406, 582
AcoI YGGCCR 2 cut(s) 183, 518
AcsI RAATTY 2 cut(s) 263, 404
AcuI CTGAAG 2 cut(s) 240, 440
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 2 cut(s) 285, 461
AfiI CCNNNNNNNGG 2 cut(s) 314, 968
AgsI TTSAA 2 cut(s) 908, 914
AjnI CCWGG 3 cut(s) 307, 520, 961
AluBI AGCT 8 cut(s) 116, 130, 376, 388, 450, 479, 485, 934
AluI AGCT 8 cut(s) 116, 130, 376, 388, 450, 479, 485, 934
AlwI GGATC 5 cut(s) 320, 326, 393, 406, 582
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 2 cut(s) 95, 569
AoxI GGCC 6 cut(s) 57, 100, 150, 183, 518, 743
ApaI GGGCCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 757
ApoI RAATTY 2 cut(s) 263, 404
AspS9I GGNCC 3 cut(s) 100, 101, 861
AsuC2I CCSGG 5 cut(s) 96, 97, 570, 571, 902
AsuHPI GGTGA 3 cut(s) 329, 590, 893
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 2 cut(s) 95, 569
AvaII GGWCC 1 cut(s) 861
BaeGI GKGCMC 3 cut(s) 104, 586, 975
BalI TGGCCA 2 cut(s) 185, 520
BamHI GGATCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 769
BccI CCATC 6 cut(s) 116, 131, 789, 804, 876, 986
BciT130I CCWGG 3 cut(s) 309, 522, 963
BcnI CCSGG 5 cut(s) 96, 97, 570, 571, 902
BfaI CTAG 2 cut(s) 120, 476
BfmI CTRYAG 1 cut(s) 825
BisI GCNGC 1 cut(s) 758
BlsI GCNGC 1 cut(s) 759
Bme1390I CCNGG 8 cut(s) 96, 97, 309, 522, 570, 571, 902, 963
Bme18I GGWCC 1 cut(s) 861
BmeT110I CYCGRG 2 cut(s) 95, 569
BmgT120I GGNCC 3 cut(s) 100, 101, 861
BmiI GGNNCC 5 cut(s) 91, 101, 102, 400, 862
BmrFI CCNGG 8 cut(s) 96, 97, 309, 522, 570, 571, 902, 963
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 3 cut(s) 223, 538, 705
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 3 cut(s) 466, 501, 670
Bpu10I CCTNAGC 1 cut(s) 336
BpuMI CCSGG 5 cut(s) 96, 97, 570, 571, 902
BsaAI YACGTR 1 cut(s) 707
BsaBI GATNNNNATC 2 cut(s) 330, 397
BsaJI CCNNGG 8 cut(s) 94, 95, 96, 568, 569, 570, 608, 938
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 968
Bse1I ACTGG 4 cut(s) 518, 626, 687, 1033
Bse8I GATNNNNATC 2 cut(s) 330, 397
BseBI CCWGG 3 cut(s) 309, 522, 963
BseDI CCNNGG 8 cut(s) 94, 95, 96, 568, 569, 570, 608, 938
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 2 cut(s) 330, 397
BseLI CCNNNNNNNGG 2 cut(s) 314, 968
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 4 cut(s) 518, 626, 687, 1033
BseSI GKGCMC 3 cut(s) 104, 586, 975
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 968
BshFI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BsiHKCI CYCGRG 2 cut(s) 95, 569
BsiSI CCGG 3 cut(s) 96, 570, 902
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 2 cut(s) 314, 968
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BsoBI CYCGRG 2 cut(s) 95, 569
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 104, 586, 975
Bsp143I GATC 4 cut(s) 325, 331, 398, 574
Bsp19I CCATGG 2 cut(s) 608, 938
BspACI CCGC 2 cut(s) 68, 87
BspANI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BspCNI CTCAG 1 cut(s) 646
BspLI GGNNCC 5 cut(s) 91, 101, 102, 400, 862
BspOI GCTAGC 1 cut(s) 479
BspPI GGATC 5 cut(s) 320, 326, 393, 406, 582
BspQI GCTCTTC 2 cut(s) 226, 366
BsrI ACTGG 4 cut(s) 518, 626, 687, 1033
BssECI CCNNGG 8 cut(s) 94, 95, 96, 568, 569, 570, 608, 938
BssMI GATC 4 cut(s) 325, 331, 398, 574
BssNAI GTATAC 1 cut(s) 769
BssT1I CCWWGG 2 cut(s) 608, 938
Bst1107I GTATAC 1 cut(s) 769
Bst2UI CCWGG 3 cut(s) 309, 522, 963
Bst4CI ACNGT 2 cut(s) 346, 492
Bst6I CTCTTC 4 cut(s) 226, 267, 344, 366
BstAPI GCANNNNNTGC 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 4 cut(s) 42, 378, 477, 720
BstDEI CTNAG 3 cut(s) 131, 336, 633
BstDSI CCRYGG 2 cut(s) 608, 938
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 4 cut(s) 328, 334, 401, 577
BstMBI GATC 4 cut(s) 325, 331, 398, 574
BstMWI GCNNNNNNNGC 2 cut(s) 56, 242
BstNI CCWGG 3 cut(s) 309, 522, 963
BstSCI CCNGG 8 cut(s) 94, 95, 307, 520, 568, 569, 900, 961
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 3 cut(s) 104, 586, 975
BstSNI TACGTA 1 cut(s) 707
BstV1I GCAGC 1 cut(s) 769
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 2 cut(s) 331, 398
BstYI RGATCY 2 cut(s) 331, 398
BstZ17I GTATAC 1 cut(s) 769
BsuRI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BtgI CCRYGG 2 cut(s) 608, 938
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 351, 488
Cac8I GCNNGC 4 cut(s) 42, 378, 477, 720
CaiI CAGNNNCTG 1 cut(s) 968
Cfr13I GGNCC 3 cut(s) 100, 101, 861
Cfr9I CCCGGG 2 cut(s) 95, 569
CsiI ACCWGGT 1 cut(s) 961
Csp6I GTAC 2 cut(s) 284, 460
CviQI GTAC 2 cut(s) 284, 460
DdeI CTNAG 3 cut(s) 131, 336, 633
DpnI GATC 4 cut(s) 327, 333, 400, 576
DpnII GATC 4 cut(s) 325, 331, 398, 574
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 2 cut(s) 183, 518
Eam1104I CTCTTC 4 cut(s) 226, 267, 344, 366
EarI CTCTTC 4 cut(s) 226, 267, 344, 366
Eco105I TACGTA 1 cut(s) 707
Eco130I CCWWGG 2 cut(s) 608, 938
Eco24I GRGCYC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 861
Eco57I CTGAAG 2 cut(s) 240, 440
Eco88I CYCGRG 2 cut(s) 95, 569
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 307, 520, 961
EcoT14I CCWWGG 2 cut(s) 608, 938
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 608, 938
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 879, 911
FaqI GGGAC 1 cut(s) 884
FblI GTMKAC 2 cut(s) 542, 768
Fnu4HI GCNGC 1 cut(s) 758
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 1 cut(s) 758
FspBI CTAG 2 cut(s) 120, 476
GluI GCNGC 1 cut(s) 758
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 3 cut(s) 466, 501, 670
HaeIII GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
HapII CCGG 3 cut(s) 96, 570, 902
HincII GTYRAC 2 cut(s) 508, 543
HindII GTYRAC 2 cut(s) 508, 543
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 2 cut(s) 393, 773
HpaII CCGG 3 cut(s) 96, 570, 902
HphI GGTGA 3 cut(s) 329, 590, 893
Hpy166II GTNNAC 6 cut(s) 286, 434, 508, 543, 671, 769
Hpy188I TCNGA 4 cut(s) 78, 392, 398, 636
Hpy188III TCNNGA 6 cut(s) 225, 329, 445, 692, 785, 835
Hpy8I GTNNAC 6 cut(s) 286, 434, 508, 543, 671, 769
HpyAV CCTTC 3 cut(s) 264, 657, 851
HpyCH4III ACNGT 2 cut(s) 346, 492
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 3 cut(s) 236, 718, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 242
HpyF3I CTNAG 3 cut(s) 131, 336, 633
HpySE526I ACGT 1 cut(s) 706
Kzo9I GATC 4 cut(s) 325, 331, 398, 574
LguI GCTCTTC 2 cut(s) 226, 366
LmnI GCTCC 5 cut(s) 121, 160, 447, 482, 502
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 3 cut(s) 223, 538, 705
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 2 cut(s) 120, 476
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 785, 957
MalI GATC 4 cut(s) 327, 333, 400, 576
MboI GATC 4 cut(s) 325, 331, 398, 574
MboII GAAGA 9 cut(s) 91, 153, 213, 284, 361, 383, 446, 724, 899
MflI RGATCY 2 cut(s) 331, 398
MhlI GDGCHC 3 cut(s) 104, 586, 975
MlsI TGGCCA 2 cut(s) 185, 520
MluCI AATT 3 cut(s) 263, 404, 638
MluNI TGGCCA 2 cut(s) 185, 520
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 2 cut(s) 13, 397
MnlI CCTC 2 cut(s) 84, 477
Mox20I TGGCCA 2 cut(s) 185, 520
MscI TGGCCA 2 cut(s) 185, 520
MseI TTAA 1 cut(s) 1013
MslI CAYNNNNRTG 1 cut(s) 622
Msp20I TGGCCA 2 cut(s) 185, 520
MspI CCGG 3 cut(s) 96, 570, 902
MspR9I CCNGG 8 cut(s) 96, 97, 309, 522, 570, 571, 902, 963
MvaI CCWGG 3 cut(s) 309, 522, 963
MwoI GCNNNNNNNGC 2 cut(s) 56, 242
NciI CCSGG 5 cut(s) 96, 97, 570, 571, 902
NcoI CCATGG 2 cut(s) 608, 938
NdeII GATC 4 cut(s) 325, 331, 398, 574
NheI GCTAGC 1 cut(s) 475
NlaIV GGNNCC 5 cut(s) 91, 101, 102, 400, 862
NmuCI GTSAC 1 cut(s) 785
PciSI GCTCTTC 2 cut(s) 226, 366
PfeI GAWTC 1 cut(s) 393
PflMI CCANNNNNTGG 2 cut(s) 314, 968
PkrI GCNGC 1 cut(s) 759
PleI GAGTC 1 cut(s) 781
PpsI GAGTC 1 cut(s) 781
Ppu21I YACGTR 1 cut(s) 707
Psp6I CCWGG 3 cut(s) 307, 520, 961
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 3 cut(s) 307, 520, 961
PspN4I GGNNCC 5 cut(s) 91, 101, 102, 400, 862
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 3 cut(s) 100, 101, 861
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 2 cut(s) 331, 398
RsaI GTAC 2 cut(s) 285, 461
RsaNI GTAC 2 cut(s) 284, 460
RseI CAYNNNNRTG 1 cut(s) 622
SalI GTCGAC 1 cut(s) 541
SapI GCTCTTC 2 cut(s) 226, 366
SaqAI TTAA 1 cut(s) 1013
SatI GCNGC 1 cut(s) 758
Sau3AI GATC 4 cut(s) 325, 331, 398, 574
Sau96I GGNCC 3 cut(s) 100, 101, 861
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 8 cut(s) 96, 97, 309, 522, 570, 571, 902, 963
SduI GDGCHC 3 cut(s) 104, 586, 975
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 3 cut(s) 223, 538, 705
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 1 cut(s) 861
SmaI CCCGGG 2 cut(s) 97, 571
SmiMI CAYNNNNRTG 1 cut(s) 622
SnaBI TACGTA 1 cut(s) 707
Sse9I AATT 3 cut(s) 263, 404, 638
SsiI CCGC 2 cut(s) 68, 87
SspMI CTAG 2 cut(s) 120, 476
StyD4I CCNGG 8 cut(s) 94, 95, 307, 520, 568, 569, 900, 961
StyI CCWWGG 2 cut(s) 608, 938
TaaI ACNGT 2 cut(s) 346, 492
TaiI ACGT 1 cut(s) 709
TaqI TCGA 5 cut(s) 173, 270, 542, 589, 776
TasI AATT 3 cut(s) 263, 404, 638
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 1 cut(s) 1013
Tru9I TTAA 1 cut(s) 1013
TscAI CASTG 3 cut(s) 243, 351, 495
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 1 cut(s) 757
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 5 cut(s) 197, 639, 870, 900, 1017
TspGWI ACGGA 1 cut(s) 853
TspMI CCCGGG 2 cut(s) 95, 569
TspRI CASTG 3 cut(s) 243, 351, 495
Van91I CCANNNNNTGG 2 cut(s) 314, 968
VpaK11BI GGWCC 1 cut(s) 861
XapI RAATTY 2 cut(s) 263, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmaI CCCGGG 2 cut(s) 95, 569
XmiI GTMKAC 2 cut(s) 542, 768
XspI CTAG 2 cut(s) 120, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.