Rroxscaffold_6G00408360

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000873
Physical Location & Seq
Reverse (-)
30786888 .. 30792407
5520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408360.1

Sequence Viewer

Length: 1131 bp
ATGGCCAGTGGAGTTAACCGGAAGATATCGGCCGCTTCGGCCAGAGCTCACACCCGAAGAACCAAGCAAAACAGCTCCTTCCGCCTTCCTTCAGGGATGTTTAGGACAACACTGGTGGTGTTGTTTATGGGGTTTCTGGCATGGGCATATCAGGCCATCCAACCTCCTCCACCAAAGATTTGTGGCTCTCCTGATGGCCCTCCTGTTACAGCACCAAGAATAAAACTTAGGGATGGAAGGCATTTAGCCTATCAAGAGCATGGGGTCCCAAAAGAAAATGCTCAGTATAAAATTGTTTATGTCCATGGTATCGATTCTTGCAGACATGATGCTGTTGCTGCACAAACCCTATCTCCAGAAATTGTTGAAGACTTGGGTGTCTACTTTGTGTCTTTTGACAGACCTGGTTATGGAGAGAGTGATCCTGATCCAAAGAGAACAGCGAAGAGTATGGCTTTAGATATAGAGGAGCTGGCTGATGAATTAGGATTAGGATCCAGATTCTATGTAATTGGTTTTTCCATGGGTGGACAGGTTCTTTGGGGTTGCCTCAAGTACATCCCTCACAGGCTAGCAGGAGCGGCACTATTAGCTCCAGTTGTTAATTACTGGTGGACTGGTATTCCTGCAAATCTATCTAACGAAGCATACCACCAACAGTTTCAGCAAGACCAATGGGCAGTTCGTGTTTCTCACTACACCCCTTGGCTTACCTATTTCTGGAACACTCAAAAATGGTTTCCCGCTTCAAGTGTTATGGCTCACAGCACAGATATTCTTTCTCAACAAGACAAAGAACTCCTCCCTAAGCTTTTCAAAAGAAAACCATATATGGACCAGGTAAGACAGCAAGGAGAATTTGAGTCCATTCACCGTGACATGAATATTGGATTTGCGACCTGGGAATTCAGTCCTCTGGATCTGGAAAATCCTTTTCCAAACAATGAAGCTTCTGTTCACGTGTGGCATGGAGATGAAGATAACATGGTTCCTGTTACTCTGCAACGATACATTGCCCAACAACTTCCCTGGATTCAATATCATGAGATACCAGGTGCTGGACACCTGTTCCCACATGCTGATGGGATGTGCAATACCATTGTCAAGGCACTTTTAACTGATGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

376

Amino Acids

42.58

Weight (kDa)

6.56

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 98 - 360 6.5e-16 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 121 - 357 1.9e-13 alpha/beta hydrolase fold
Hydrolase_4 PF12146 123 - 356 1.5e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccBSI CCGCTC 1 cut(s) 581
AccI GTMKAC 1 cut(s) 381
AciI CCGC 4 cut(s) 33, 82, 581, 744
AclWI GGATC 5 cut(s) 416, 422, 489, 502, 927
AcoI YGGCCR 3 cut(s) 3, 30, 39
AcsI RAATTY 2 cut(s) 857, 905
AcuI CTGAAG 1 cut(s) 75
AcvI CACGTG 1 cut(s) 961
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 3 cut(s) 410, 720, 1058
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 4 cut(s) 368, 750, 817, 1037
AjnI CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 6 cut(s) 47, 75, 472, 593, 811, 950
AluI AGCT 6 cut(s) 47, 75, 472, 593, 811, 950
Alw21I GWGCWC 1 cut(s) 49
AlwI GGATC 5 cut(s) 416, 422, 489, 502, 927
AlwNI CAGNNNCTG 1 cut(s) 1058
AoxI GGCC 5 cut(s) 3, 30, 39, 153, 196
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 2 cut(s) 857, 905
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
AspS9I GGNCC 3 cut(s) 197, 265, 835
AsuHPI GGTGA 1 cut(s) 863
AsuNHI GCTAGC 1 cut(s) 571
AvaII GGWCC 2 cut(s) 265, 835
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 49
BbrPI CACGTG 1 cut(s) 961
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 325
BccI CCATC 4 cut(s) 164, 188, 227, 1076
BciT130I CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BfaI CTAG 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 3 cut(s) 33, 339, 582
BlsI GCNGC 3 cut(s) 34, 340, 583
Bme1390I CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
Bme18I GGWCC 2 cut(s) 265, 835
BmgT120I GGNCC 3 cut(s) 197, 265, 835
BmiI GGNNCC 4 cut(s) 266, 267, 496, 990
BmrFI CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
BmsI GCATC 1 cut(s) 319
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 2 cut(s) 339, 579
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 536
Bsa29I ATCGAT 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 961
BsaBI GATNNNNATC 2 cut(s) 426, 493
BsaJI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BsaWI WCCGGW 1 cut(s) 18
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 720, 1058
Bse1I ACTGG 5 cut(s) 6, 117, 596, 614, 622
Bse3DI GCAATG 1 cut(s) 1011
Bse8I GATNNNNATC 2 cut(s) 426, 493
BseBI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BseGI GGATG 5 cut(s) 102, 156, 238, 558, 1092
BseJI GATNNNNATC 2 cut(s) 426, 493
BseLI CCNNNNNNNGG 3 cut(s) 410, 720, 1058
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 5 cut(s) 6, 117, 596, 614, 622
BseRI GAGGAG 3 cut(s) 156, 482, 791
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 325
BsgI GTGCAG 1 cut(s) 324
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 5, 32, 41, 155, 198
BshVI ATCGAT 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 19
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 3 cut(s) 410, 720, 1058
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 5 cut(s) 5, 32, 41, 155, 198
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 4 cut(s) 421, 427, 494, 919
Bsp19I CCATGG 2 cut(s) 304, 522
BspACI CCGC 4 cut(s) 33, 82, 581, 744
BspANI GGCC 5 cut(s) 5, 32, 41, 155, 198
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 312
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 4 cut(s) 266, 267, 496, 990
BspOI GCTAGC 1 cut(s) 575
BspPI GGATC 5 cut(s) 416, 422, 489, 502, 927
BsrBI CCGCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 1011
BsrI ACTGG 5 cut(s) 6, 117, 596, 614, 622
BssECI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BssMI GATC 4 cut(s) 421, 427, 494, 919
BssT1I CCWWGG 3 cut(s) 304, 522, 704
Bst2UI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
Bst4CI ACNGT 2 cut(s) 660, 875
Bst6I CTCTTC 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 961
BstC8I GCNNGC 2 cut(s) 474, 573
BstDEI CTNAG 3 cut(s) 227, 282, 807
BstDSI CCRYGG 2 cut(s) 304, 522
BstF5I GGATG 5 cut(s) 102, 156, 238, 558, 1092
BstKTI GATC 4 cut(s) 424, 430, 497, 922
BstMBI GATC 4 cut(s) 421, 427, 494, 919
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 6 cut(s) 38, 81, 152, 338, 581, 590
BstNI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BstNSI RCATGY 1 cut(s) 1079
BstSCI CCNGG 5 cut(s) 403, 837, 899, 1028, 1051
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 494, 919
BstYI RGATCY 2 cut(s) 494, 919
BstZI CGGCCG 1 cut(s) 30
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 5 cut(s) 5, 32, 41, 155, 198
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 2 cut(s) 304, 522
BtsCI GGATG 5 cut(s) 102, 156, 238, 558, 1092
BtsIMutI CAGTG 2 cut(s) 13, 110
Cac8I GCNNGC 2 cut(s) 474, 573
CaiI CAGNNNCTG 1 cut(s) 1058
CciI TCATGA 1 cut(s) 1042
Cfr13I GGNCC 3 cut(s) 197, 265, 835
ClaI ATCGAT 1 cut(s) 312
CsiI ACCWGGT 3 cut(s) 403, 837, 1051
Csp6I GTAC 1 cut(s) 556
CspCI CAANNNNNGTGG 2 cut(s) 96, 131
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 3 cut(s) 227, 282, 807
DpnI GATC 4 cut(s) 423, 429, 496, 921
DpnII GATC 4 cut(s) 421, 427, 494, 919
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 3 cut(s) 3, 30, 39
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 47
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 304, 522, 704
Eco24I GRGCYC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 265, 835
Eco52I CGGCCG 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 75
Eco72I CACGTG 1 cut(s) 961
EcoICRI GAGCTC 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 3 cut(s) 304, 522, 704
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 3 cut(s) 304, 522, 704
FaqI GGGAC 1 cut(s) 251
FauI CCCGC 1 cut(s) 751
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 33, 339, 582
FokI GGATG 5 cut(s) 109, 143, 245, 545, 1099
FriOI GRGCYC 1 cut(s) 49
Fsp4HI GCNGC 3 cut(s) 33, 339, 582
FspBI CTAG 1 cut(s) 572
GluI GCNGC 3 cut(s) 33, 339, 582
GsuI CTGGAG 2 cut(s) 339, 579
HaeIII GGCC 5 cut(s) 5, 32, 41, 155, 198
HapII CCGG 1 cut(s) 19
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 2 cut(s) 809, 948
HinfI GANTC 4 cut(s) 314, 501, 863, 1033
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 1 cut(s) 19
HphI GGTGA 1 cut(s) 863
Hpy166II GTNNAC 5 cut(s) 16, 382, 530, 615, 958
Hpy188III TCNNGA 9 cut(s) 191, 254, 356, 425, 498, 721, 917, 923, 1043
Hpy8I GTNNAC 5 cut(s) 16, 382, 530, 615, 958
HpyAV CCTTC 4 cut(s) 88, 95, 99, 231
HpyCH4III ACNGT 2 cut(s) 660, 875
HpyCH4IV ACGT 1 cut(s) 960
HpyCH4V TGCA 5 cut(s) 321, 341, 629, 1003, 1092
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 81, 152, 338, 581, 590
HpyF3I CTNAG 3 cut(s) 227, 282, 807
HpySE526I ACGT 1 cut(s) 960
KflI GGGWCCC 1 cut(s) 265
KspAI GTTAAC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 421, 427, 494, 919
LmnI GCTCC 4 cut(s) 80, 469, 578, 598
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 319
MabI ACCWGGT 3 cut(s) 403, 837, 1051
MaeI CTAG 1 cut(s) 572
MaeII ACGT 1 cut(s) 960
MaeIII GTNAC 3 cut(s) 205, 875, 994
MalI GATC 4 cut(s) 423, 429, 496, 921
MbiI CCGCTC 1 cut(s) 581
MboI GATC 4 cut(s) 421, 427, 494, 919
MboII GAAGA 5 cut(s) 34, 69, 380, 457, 989
MflI RGATCY 2 cut(s) 494, 919
MhlI GDGCHC 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 291, 360, 482, 510, 604, 857, 905
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 8 cut(s) 174, 177, 210, 460, 560, 573, 812, 924
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 15, 603, 1115
MslI CAYNNNNRTG 2 cut(s) 972, 1080
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 19
MspR9I CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
MvaI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
MwoI GCNNNNNNNGC 6 cut(s) 38, 81, 152, 338, 581, 590
NcoI CCATGG 2 cut(s) 304, 522
NdeII GATC 4 cut(s) 421, 427, 494, 919
NheI GCTAGC 1 cut(s) 571
NlaIV GGNNCC 4 cut(s) 266, 267, 496, 990
NmuCI GTSAC 1 cut(s) 875
NspI RCATGY 1 cut(s) 1079
PagI TCATGA 1 cut(s) 1042
PfeI GAWTC 3 cut(s) 314, 501, 1033
PflMI CCANNNNNTGG 1 cut(s) 1058
PkrI GCNGC 3 cut(s) 34, 340, 583
PleI GAGTC 1 cut(s) 871
PmaCI CACGTG 1 cut(s) 961
PmlI CACGTG 1 cut(s) 961
PpsI GAGTC 1 cut(s) 871
Ppu21I YACGTR 1 cut(s) 961
PpuMI RGGWCCY 1 cut(s) 265
Psp124BI GAGCTC 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
PspCI CACGTG 1 cut(s) 961
PspGI CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
PspN4I GGNNCC 4 cut(s) 266, 267, 496, 990
PspPI GGNCC 3 cut(s) 197, 265, 835
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 2 cut(s) 494, 919
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
RseI CAYNNNNRTG 2 cut(s) 972, 1080
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 15, 603, 1115
SatI GCNGC 3 cut(s) 33, 339, 582
Sau3AI GATC 4 cut(s) 421, 427, 494, 919
Sau96I GGNCC 3 cut(s) 197, 265, 835
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
SduI GDGCHC 1 cut(s) 49
SexAI ACCWGGT 3 cut(s) 403, 837, 1051
SfaNI GCATC 1 cut(s) 319
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SinI GGWCC 2 cut(s) 265, 835
SmiMI CAYNNNNRTG 2 cut(s) 972, 1080
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 7 cut(s) 291, 360, 482, 510, 604, 857, 905
SsiI CCGC 4 cut(s) 33, 82, 581, 744
SspI AATATT 1 cut(s) 886
SspMI CTAG 1 cut(s) 572
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 5 cut(s) 403, 837, 899, 1028, 1051
StyI CCWWGG 3 cut(s) 304, 522, 704
TaaI ACNGT 2 cut(s) 660, 875
TaiI ACGT 1 cut(s) 963
TaqI TCGA 1 cut(s) 312
TasI AATT 7 cut(s) 291, 360, 482, 510, 604, 857, 905
TatI WGTACW 1 cut(s) 555
TauI GCSGC 2 cut(s) 35, 584
TfiI GAWTC 3 cut(s) 314, 501, 1033
Tru1I TTAA 3 cut(s) 15, 603, 1115
Tru9I TTAA 3 cut(s) 15, 603, 1115
TscAI CASTG 2 cut(s) 13, 117
TseFI GTSAC 1 cut(s) 875
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 875
TspDTI ATGAA 4 cut(s) 495, 896, 960, 990
TspRI CASTG 2 cut(s) 13, 117
Van91I CCANNNNNTGG 1 cut(s) 1058
VpaK11BI GGWCC 2 cut(s) 265, 835
XapI RAATTY 2 cut(s) 857, 905
XceI RCATGY 1 cut(s) 1079
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.