Rroxscaffold_4G00318150

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000873
Physical Location & Seq
Forward (+)
44048528 .. 44050531
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318150.1

Sequence Viewer

Length: 930 bp
ATGGGCTTATCAGGCCACCCAACCGCCCCTCCGAAGATTTGCGGTTCCCCCGGCGGCCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCCTACAAGGAGCACGGTGTGTCGAAACACGTGGCCAGATATAAGATTATCTTTGTTCATGGCTTTAGTTCTTCCAGACATGATGCAGAAGTTATTGAAGAGTTAGGGGTACACATTGTGTCTTTTGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGTTCCAAATTTTATGTAGTTGGATATTCTATGGGTGGACAGGTTATCTGGAGCTGTCTTCAGTACATCCCTCATAGGCTAGCTGGAGCTACACTGTATGCTCCTGTTGTCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCAACAGATGCCTACTATAAACAACCCCCCGGGACCAATGGGCACTTCGAGACTTGGAAATACTTGGGAATATGCTGGCTTCTGGTATACAAACGAACAGGTGAAAGAGTAGGTCTCTTGCAACCAGACTGTGACGATATTATTAATTTGTTTTTGCCGCAGCCACATGGAAAGCAGCAAGGAGTATACGAGTCGAACCATCGTGACTTGATGATTGGATTTGGGAGATGGGAGTTTGACCCTATAGAAATCAAGAACCCATTTCCCAACAATGAAGGGTCCGTCCATCTGTGGCAGGGTGATGAAGATAGGCTTGTCCCAGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTGACTACCATGAGTTACCAGGTGCTGGGCATTTGTTTGCATATAACGATGGTATAAATGAAGCTATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

309

Amino Acids

34.76

Weight (kDa)

6.22

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 48 - 151 5.6e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 51 - 166 2.9e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 51 - 291 7.3e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 245, 857
AccI GTMKAC 2 cut(s) 558, 657
AciI CCGC 4 cut(s) 24, 42, 54, 629
AclWI GGATC 2 cut(s) 251, 257
AcoI YGGCCR 2 cut(s) 138, 449
AcsI RAATTY 1 cut(s) 335
AcuI CTGAAG 1 cut(s) 371
AcvI CACGTG 1 cut(s) 136
AdeI CACNNNGTG 2 cut(s) 124, 223
AfaI GTAC 2 cut(s) 216, 392
AfiI CCNNNNNNNGG 2 cut(s) 245, 857
AflIII ACRYGT 1 cut(s) 133
AgsI TTSAA 3 cut(s) 203, 797, 803
AjnI CCWGG 3 cut(s) 238, 451, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 9 cut(s) 71, 85, 307, 319, 381, 410, 416, 823, 896
AluI AGCT 9 cut(s) 71, 85, 307, 319, 381, 410, 416, 823, 896
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 515, 590
AlwI GGATC 2 cut(s) 251, 257
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 500
AoxI GGCC 5 cut(s) 13, 55, 105, 138, 449
ApeKI GCWGC 2 cut(s) 631, 646
ApoI RAATTY 1 cut(s) 335
AseI ATTAAT 1 cut(s) 615
AspS9I GGNCC 3 cut(s) 56, 504, 750
AsuC2I CCSGG 3 cut(s) 51, 501, 502
AsuHPI GGTGA 3 cut(s) 260, 584, 782
AsuNHI GCTAGC 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 500
AvaII GGWCC 2 cut(s) 504, 750
BaeGI GKGCMC 1 cut(s) 516
BalI TGGCCA 2 cut(s) 140, 451
BbrPI CACGTG 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 643, 658
BccI CCATC 6 cut(s) 71, 86, 678, 693, 765, 875
BciT130I CCWGG 3 cut(s) 240, 453, 852
BcnI CCSGG 3 cut(s) 51, 501, 502
BcoDI GTCTC 2 cut(s) 515, 590
BfaI CTAG 2 cut(s) 75, 407
BfmI CTRYAG 1 cut(s) 714
BisI GCNGC 4 cut(s) 55, 629, 632, 647
BlsI GCNGC 4 cut(s) 56, 630, 633, 648
Bme1390I CCNGG 6 cut(s) 51, 240, 453, 501, 502, 852
Bme18I GGWCC 2 cut(s) 504, 750
BmeT110I CYCGRG 1 cut(s) 500
BmgT120I GGNCC 3 cut(s) 56, 504, 750
BmiI GGNNCC 4 cut(s) 46, 331, 505, 751
BmrFI CCNGG 6 cut(s) 51, 240, 453, 501, 502, 852
BmrI ACTGGG 2 cut(s) 785, 927
BmsI GCATC 2 cut(s) 178, 469
BmtI GCTAGC 1 cut(s) 410
BmuI ACTGGG 2 cut(s) 785, 927
BpiI GAAGAC 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 570, 602
BpmI CTGGAG 2 cut(s) 397, 432
Bpu10I CCTNAGC 1 cut(s) 267
BpuMI CCSGG 3 cut(s) 51, 501, 502
BsaAI YACGTR 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 4 cut(s) 49, 499, 500, 827
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 857
Bse1I ACTGG 3 cut(s) 449, 791, 922
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 3 cut(s) 240, 453, 852
BseDI CCNNGG 4 cut(s) 49, 499, 500, 827
BseGI GGATG 1 cut(s) 393
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 2 cut(s) 245, 857
BseNI ACTGG 3 cut(s) 449, 791, 922
BseSI GKGCMC 1 cut(s) 516
BseXI GCAGC 2 cut(s) 643, 658
BseYI CCCAGC 1 cut(s) 857
BshFI GGCC 5 cut(s) 15, 57, 107, 140, 451
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 500
BsiSI CCGG 2 cut(s) 51, 501
BslFI GGGAC 2 cut(s) 517, 773
BslI CCNNNNNNNGG 2 cut(s) 245, 857
BsmAI GTCTC 2 cut(s) 515, 590
BsmFI GGGAC 2 cut(s) 517, 773
BsnI GGCC 5 cut(s) 15, 57, 107, 140, 451
Bso31I GGTCTC 1 cut(s) 590
BsoBI CYCGRG 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 120, 516
Bsp143I GATC 2 cut(s) 256, 262
Bsp19I CCATGG 1 cut(s) 827
BspACI CCGC 4 cut(s) 24, 42, 54, 629
BspANI GGCC 5 cut(s) 15, 57, 107, 140, 451
BspLI GGNNCC 4 cut(s) 46, 331, 505, 751
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 2 cut(s) 251, 257
BspQI GCTCTTC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 590
BsrI ACTGG 3 cut(s) 449, 791, 922
BssECI CCNNGG 4 cut(s) 49, 499, 500, 827
BssMI GATC 2 cut(s) 256, 262
BssNAI GTATAC 2 cut(s) 559, 658
BssT1I CCWWGG 1 cut(s) 827
Bst1107I GTATAC 2 cut(s) 559, 658
Bst2UI CCWGG 3 cut(s) 240, 453, 852
Bst4CI ACNGT 4 cut(s) 122, 277, 423, 602
Bst6I CTCTTC 3 cut(s) 198, 275, 297
BstBAI YACGTR 1 cut(s) 136
BstC8I GCNNGC 3 cut(s) 309, 408, 548
BstDEI CTNAG 2 cut(s) 86, 267
BstDSI CCRYGG 1 cut(s) 827
BstF5I GGATG 1 cut(s) 393
BstKTI GATC 2 cut(s) 259, 265
BstMAI GTCTC 2 cut(s) 515, 590
BstMBI GATC 2 cut(s) 256, 262
BstMWI GCNNNNNNNGC 1 cut(s) 12
BstNI CCWGG 3 cut(s) 240, 453, 852
BstSCI CCNGG 6 cut(s) 49, 238, 451, 499, 500, 850
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 516
BstV1I GCAGC 2 cut(s) 643, 658
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BstZ17I GTATAC 2 cut(s) 559, 658
BsuRI GGCC 5 cut(s) 15, 57, 107, 140, 451
BtgI CCRYGG 1 cut(s) 827
BtsCI GGATG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 282, 419
Cac8I GCNNGC 3 cut(s) 309, 408, 548
CaiI CAGNNNCTG 1 cut(s) 857
Cfr13I GGNCC 3 cut(s) 56, 504, 750
Cfr9I CCCGGG 1 cut(s) 500
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 2 cut(s) 215, 391
CviAII CATG 5 cut(s) 164, 185, 638, 828, 842
CviQI GTAC 2 cut(s) 215, 391
DdeI CTNAG 2 cut(s) 86, 267
DpnI GATC 2 cut(s) 258, 264
DpnII GATC 2 cut(s) 256, 262
DraIII CACNNNGTG 2 cut(s) 124, 223
EaeI YGGCCR 2 cut(s) 138, 449
Eam1104I CTCTTC 3 cut(s) 198, 275, 297
EarI CTCTTC 3 cut(s) 198, 275, 297
Eco130I CCWWGG 1 cut(s) 827
Eco31I GGTCTC 1 cut(s) 590
Eco47I GGWCC 2 cut(s) 504, 750
Eco57I CTGAAG 1 cut(s) 371
Eco72I CACGTG 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 238, 451, 850
EcoT14I CCWWGG 1 cut(s) 827
ErhI CCWWGG 1 cut(s) 827
FaeI CATG 5 cut(s) 167, 188, 641, 831, 845
FalI AAGNNNNNCTT 4 cut(s) 140, 172, 768, 800
FaqI GGGAC 2 cut(s) 517, 773
FatI CATG 5 cut(s) 163, 184, 637, 827, 841
FblI GTMKAC 2 cut(s) 558, 657
Fnu4HI GCNGC 4 cut(s) 55, 629, 632, 647
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 4 cut(s) 55, 629, 632, 647
FspBI CTAG 2 cut(s) 75, 407
GluI GCNGC 4 cut(s) 55, 629, 632, 647
GsaI CCCAGC 1 cut(s) 861
GsuI CTGGAG 2 cut(s) 397, 432
HaeIII GGCC 5 cut(s) 15, 57, 107, 140, 451
HapII CCGG 2 cut(s) 51, 501
Hin1II CATG 5 cut(s) 167, 188, 641, 831, 845
HincII GTYRAC 2 cut(s) 439, 474
HindII GTYRAC 2 cut(s) 439, 474
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 2 cut(s) 324, 662
HpaII CCGG 2 cut(s) 51, 501
HphI GGTGA 3 cut(s) 260, 584, 782
Hpy166II GTNNAC 6 cut(s) 217, 365, 439, 474, 559, 658
Hpy188I TCNGA 2 cut(s) 33, 323
Hpy188III TCNNGA 6 cut(s) 180, 260, 376, 520, 674, 724
Hpy8I GTNNAC 6 cut(s) 217, 365, 439, 474, 559, 658
HpyAV CCTTC 1 cut(s) 740
HpyCH4III ACNGT 4 cut(s) 122, 277, 423, 602
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 3 cut(s) 191, 592, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 12
HpyF3I CTNAG 2 cut(s) 86, 267
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 5 cut(s) 167, 188, 641, 831, 845
Kzo9I GATC 2 cut(s) 256, 262
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 5 cut(s) 76, 115, 378, 413, 433
Lsp1109I GCAGC 2 cut(s) 643, 658
LweI GCATC 2 cut(s) 178, 469
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 2 cut(s) 75, 407
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 602, 674, 846
MalI GATC 2 cut(s) 258, 264
MboI GATC 2 cut(s) 256, 262
MboII GAAGA 8 cut(s) 46, 108, 168, 215, 292, 314, 377, 788
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 120, 516
MlsI TGGCCA 2 cut(s) 140, 451
MluCI AATT 2 cut(s) 335, 616
MluNI TGGCCA 2 cut(s) 140, 451
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 2 cut(s) 39, 408
Mox20I TGGCCA 2 cut(s) 140, 451
MscI TGGCCA 2 cut(s) 140, 451
MseI TTAA 2 cut(s) 615, 902
Msp20I TGGCCA 2 cut(s) 140, 451
MspI CCGG 2 cut(s) 51, 501
MspR9I CCNGG 6 cut(s) 51, 240, 453, 501, 502, 852
MvaI CCWGG 3 cut(s) 240, 453, 852
MwoI GCNNNNNNNGC 1 cut(s) 12
NciI CCSGG 3 cut(s) 51, 501, 502
NcoI CCATGG 1 cut(s) 827
NdeII GATC 2 cut(s) 256, 262
NheI GCTAGC 1 cut(s) 406
NlaIII CATG 5 cut(s) 167, 188, 641, 831, 845
NlaIV GGNNCC 4 cut(s) 46, 331, 505, 751
NmuCI GTSAC 2 cut(s) 602, 674
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 324
PflMI CCANNNNNTGG 2 cut(s) 245, 857
PkrI GCNGC 4 cut(s) 56, 630, 633, 648
PleI GAGTC 1 cut(s) 670
PmaCI CACGTG 1 cut(s) 136
PmlI CACGTG 1 cut(s) 136
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 136
PshBI ATTAAT 1 cut(s) 615
Psp6I CCWGG 3 cut(s) 238, 451, 850
PspCI CACGTG 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 3 cut(s) 238, 451, 850
PspN4I GGNNCC 4 cut(s) 46, 331, 505, 751
PspPI GGNCC 3 cut(s) 56, 504, 750
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 2 cut(s) 216, 392
RsaNI GTAC 2 cut(s) 215, 391
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 615, 902
SatI GCNGC 4 cut(s) 55, 629, 632, 647
Sau3AI GATC 2 cut(s) 256, 262
Sau96I GGNCC 3 cut(s) 56, 504, 750
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 6 cut(s) 51, 240, 453, 501, 502, 852
SduI GDGCHC 2 cut(s) 120, 516
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 2 cut(s) 178, 469
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 2 cut(s) 504, 750
SmaI CCCGGG 1 cut(s) 502
Sse9I AATT 2 cut(s) 335, 616
SsiI CCGC 4 cut(s) 24, 42, 54, 629
SspMI CTAG 2 cut(s) 75, 407
StyD4I CCNGG 6 cut(s) 49, 238, 451, 499, 500, 850
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 4 cut(s) 122, 277, 423, 602
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 128, 519, 665
TasI AATT 2 cut(s) 335, 616
TatI WGTACW 1 cut(s) 390
TauI GCSGC 2 cut(s) 57, 631
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 615, 902
Tru9I TTAA 2 cut(s) 615, 902
TscAI CASTG 2 cut(s) 282, 426
TseFI GTSAC 2 cut(s) 602, 674
TseI GCWGC 2 cut(s) 631, 646
Tsp45I GTSAC 2 cut(s) 602, 674
TspDTI ATGAA 4 cut(s) 152, 759, 789, 906
TspGWI ACGGA 1 cut(s) 742
TspMI CCCGGG 1 cut(s) 500
TspRI CASTG 2 cut(s) 282, 426
Van91I CCANNNNNTGG 2 cut(s) 245, 857
VpaK11BI GGWCC 2 cut(s) 504, 750
VspI ATTAAT 1 cut(s) 615
XapI RAATTY 1 cut(s) 335
XmaI CCCGGG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 558, 657
XspI CTAG 2 cut(s) 75, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.