Rmu_ssc0000161.1_g000006

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000161.1
OG0000873
Physical Location & Seq
Forward (+)
35578 .. 39485
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000161.1_g000006.1.cds

Sequence Viewer

Length: 1158 bp
atgacgaggaggaggaatataggaggattgggggctgaggaattgggagaggagggggggcaggactttctggggaacgctgtggatgaagaggaagatgacgaggaggaggaggacgtggttttggagagagagggagagacagagaatctgtcgagggccacccaaccgccccctccgaagatttgcggttcccccgggggcccacccatcacagctcctagaataaagctaagagatggaagatatttggcctacaaggagcatggtgtgtcgaaacaagtggccagatataagattatctttgttcatgggttcggttcttccagacatgatgcagtgattgcgaccaaaccttcagcagaatttatcgaagagttaggggtacacattgtgtctttggacagaccaggctatggtgaaagtgatccagatcctaagcggacagtgaagagtttggctttagatatagaagagcttgctgaccagctcggattcggacccaaattttatgtagttggatattctatgggtggacaggttatctggagctgtcttcagtacatccctcataggctagctggagctacactgtatgctcctgttgtcaactactggtggccaggctttcctgccaacttgtcgacagatgcctactataaacaacccctcggggatcaatgggcacttcgagttgctcaccatataccatggctaacctactggtggaacactcagaaattatttccttcatcaagcgttatagttggtaaaccagagatattctccagtcaggacttggaaatacgtaggaagatgcaggcttctggcgtacaaactaacaggccacacggaaagcagcaaggagtatacgagtcgaaccatcgtgacatgatgattggatttgggagatgggagtttgaccctatagaaatcaagaacccatttcccaacaatgaagggtccgtccatctgtggcagggtgatgaggataggcttgtcccagttgaacttcaacgctttattgtgggaaagcttccatggattgactaccatgagttaacaggtgctgggcacttgtttgcatataatgatggtataaatgaagccatgttaaagtcaatgctgaccaccagtagagggagtgtgattttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

385

Amino Acids

43.27

Weight (kDa)

5.37

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 416
AccI GTMKAC 2 cut(s) 644, 870
AciI CCGC 3 cut(s) 170, 189, 442
AclWI GGATC 3 cut(s) 422, 428, 684
AcoI YGGCCR 2 cut(s) 285, 620
AcsI RAATTY 2 cut(s) 365, 506
AcuI CTGAAG 2 cut(s) 342, 542
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 3 cut(s) 387, 563, 834
AfiI CCNNNNNNNGG 3 cut(s) 416, 726, 1140
AgsI TTSAA 2 cut(s) 1010, 1016
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 2 cut(s) 409, 622
AluBI AGCT 8 cut(s) 218, 232, 478, 490, 552, 581, 587, 1036
AluI AGCT 8 cut(s) 218, 232, 478, 490, 552, 581, 587, 1036
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 3 cut(s) 422, 428, 684
AlwNI CAGNNNCTG 1 cut(s) 1070
Ama87I CYCGRG 2 cut(s) 197, 671
AoxI GGCC 6 cut(s) 159, 202, 252, 285, 620, 845
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 1 cut(s) 859
ApoI RAATTY 2 cut(s) 365, 506
AspS9I GGNCC 5 cut(s) 159, 202, 203, 500, 963
AsuC2I CCSGG 2 cut(s) 198, 199
AsuHPI GGTGA 3 cut(s) 431, 692, 995
AsuNHI GCTAGC 1 cut(s) 577
AvaI CYCGRG 2 cut(s) 197, 671
AvaII GGWCC 2 cut(s) 500, 963
BaeGI GKGCMC 3 cut(s) 206, 688, 1077
BalI TGGCCA 2 cut(s) 287, 622
BanII GRGCYC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 548
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 871
BccI CCATC 6 cut(s) 218, 233, 891, 906, 978, 1088
BciT130I CCWGG 2 cut(s) 411, 624
BcnI CCSGG 2 cut(s) 198, 199
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 2 cut(s) 222, 578
BfmI CTRYAG 1 cut(s) 927
BisI GCNGC 1 cut(s) 860
BlsI GCNGC 1 cut(s) 861
Bme1390I CCNGG 4 cut(s) 198, 199, 411, 624
Bme18I GGWCC 2 cut(s) 500, 963
BmeT110I CYCGRG 2 cut(s) 197, 671
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 5 cut(s) 159, 202, 203, 500, 963
BmiI GGNNCC 5 cut(s) 193, 203, 204, 502, 964
BmrFI CCNGG 4 cut(s) 198, 199, 411, 624
BmrI ACTGGG 1 cut(s) 998
BmsI GCATC 3 cut(s) 325, 640, 807
BmtI GCTAGC 1 cut(s) 581
BmuI ACTGGG 1 cut(s) 998
BpiI GAAGAC 1 cut(s) 548
BplI GAGNNNNNCTC 2 cut(s) 770, 802
BpmI CTGGAG 3 cut(s) 568, 603, 772
Bpu10I CCTNAGC 2 cut(s) 36, 438
BpuMI CCSGG 2 cut(s) 198, 199
BsaAI YACGTR 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 432
BsaJI CCNNGG 6 cut(s) 196, 197, 198, 670, 710, 1040
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 726, 1140
Bse1I ACTGG 5 cut(s) 620, 728, 789, 1004, 1134
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 2 cut(s) 411, 624
BseDI CCNNGG 6 cut(s) 196, 197, 198, 670, 710, 1040
BseGI GGATG 2 cut(s) 91, 564
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 3 cut(s) 416, 726, 1140
BseMII CTCAG 2 cut(s) 27, 749
BseNI ACTGG 5 cut(s) 620, 728, 789, 1004, 1134
BseRI GAGGAG 6 cut(s) 22, 25, 65, 119, 122, 125
BseSI GKGCMC 3 cut(s) 206, 688, 1077
BseXI GCAGC 1 cut(s) 871
BseYI CCCAGC 1 cut(s) 1070
BshFI GGCC 6 cut(s) 161, 204, 254, 287, 622, 847
BsiHKCI CYCGRG 2 cut(s) 197, 671
BsiSI CCGG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 3 cut(s) 416, 726, 1140
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 986
BsnI GGCC 6 cut(s) 161, 204, 254, 287, 622, 847
BsoBI CYCGRG 2 cut(s) 197, 671
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 3 cut(s) 206, 688, 1077
Bsp143I GATC 3 cut(s) 427, 433, 676
Bsp19I CCATGG 2 cut(s) 710, 1040
BspACI CCGC 3 cut(s) 170, 189, 442
BspANI GGCC 6 cut(s) 161, 204, 254, 287, 622, 847
BspCNI CTCAG 2 cut(s) 28, 748
BspLI GGNNCC 5 cut(s) 193, 203, 204, 502, 964
BspOI GCTAGC 1 cut(s) 581
BspPI GGATC 3 cut(s) 422, 428, 684
BspQI GCTCTTC 1 cut(s) 468
BsrI ACTGG 5 cut(s) 620, 728, 789, 1004, 1134
BssECI CCNNGG 6 cut(s) 196, 197, 198, 670, 710, 1040
BssMI GATC 3 cut(s) 427, 433, 676
BssNAI GTATAC 1 cut(s) 871
BssT1I CCWWGG 2 cut(s) 710, 1040
Bst1107I GTATAC 1 cut(s) 871
Bst2UI CCWGG 2 cut(s) 411, 624
Bst4CI ACNGT 2 cut(s) 448, 594
Bst6I CTCTTC 4 cut(s) 84, 369, 446, 468
BstAPI GCANNNNNTGC 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 809
BstC8I GCNNGC 3 cut(s) 480, 579, 822
BstDEI CTNAG 4 cut(s) 36, 233, 438, 735
BstDSI CCRYGG 2 cut(s) 710, 1040
BstF5I GGATG 2 cut(s) 91, 564
BstKTI GATC 3 cut(s) 430, 436, 679
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 427, 433, 676
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 2 cut(s) 411, 624
BstSCI CCNGG 4 cut(s) 196, 197, 409, 622
BstSFI CTRYAG 1 cut(s) 927
BstSLI GKGCMC 3 cut(s) 206, 688, 1077
BstSNI TACGTA 1 cut(s) 809
BstV1I GCAGC 1 cut(s) 871
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BstZ17I GTATAC 1 cut(s) 871
BsuRI GGCC 6 cut(s) 161, 204, 254, 287, 622, 847
BtgI CCRYGG 2 cut(s) 710, 1040
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 2 cut(s) 91, 564
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 345, 453, 590
Cac8I GCNNGC 3 cut(s) 480, 579, 822
CaiI CAGNNNCTG 1 cut(s) 1070
Cfr13I GGNCC 5 cut(s) 159, 202, 203, 500, 963
Cfr9I CCCGGG 1 cut(s) 197
Csp6I GTAC 3 cut(s) 386, 562, 833
CviAII CATG 8 cut(s) 266, 311, 332, 711, 892, 1041, 1055, 1111
CviQI GTAC 3 cut(s) 386, 562, 833
DdeI CTNAG 4 cut(s) 36, 233, 438, 735
DpnI GATC 3 cut(s) 429, 435, 678
DpnII GATC 3 cut(s) 427, 433, 676
DraIII CACNNNGTG 1 cut(s) 394
EaeI YGGCCR 2 cut(s) 285, 620
Eam1104I CTCTTC 4 cut(s) 84, 369, 446, 468
EarI CTCTTC 4 cut(s) 84, 369, 446, 468
Eco105I TACGTA 1 cut(s) 809
Eco130I CCWWGG 2 cut(s) 710, 1040
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 2 cut(s) 500, 963
Eco57I CTGAAG 2 cut(s) 342, 542
Eco88I CYCGRG 2 cut(s) 197, 671
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 409, 622
EcoT14I CCWWGG 2 cut(s) 710, 1040
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 2 cut(s) 710, 1040
FaeI CATG 8 cut(s) 269, 314, 335, 714, 895, 1044, 1058, 1114
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 986
FatI CATG 8 cut(s) 265, 310, 331, 710, 891, 1040, 1054, 1110
FblI GTMKAC 2 cut(s) 644, 870
Fnu4HI GCNGC 1 cut(s) 860
FokI GGATG 2 cut(s) 98, 551
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 860
FspBI CTAG 2 cut(s) 222, 578
GluI GCNGC 1 cut(s) 860
GsaI CCCAGC 1 cut(s) 1074
GsuI CTGGAG 3 cut(s) 568, 603, 772
HaeIII GGCC 6 cut(s) 161, 204, 254, 287, 622, 847
HapII CCGG 1 cut(s) 198
Hin1II CATG 8 cut(s) 269, 314, 335, 714, 895, 1044, 1058, 1114
HincII GTYRAC 3 cut(s) 610, 645, 1062
HindII GTYRAC 3 cut(s) 610, 645, 1062
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 3 cut(s) 148, 495, 875
HpaI GTTAAC 1 cut(s) 1062
HpaII CCGG 1 cut(s) 198
HphI GGTGA 3 cut(s) 431, 692, 995
Hpy166II GTNNAC 7 cut(s) 388, 536, 610, 645, 773, 871, 1062
Hpy188I TCNGA 4 cut(s) 180, 494, 500, 738
Hpy188III TCNNGA 6 cut(s) 327, 431, 547, 794, 887, 937
Hpy8I GTNNAC 7 cut(s) 388, 536, 610, 645, 773, 871, 1062
HpyAV CCTTC 3 cut(s) 366, 759, 953
HpyCH4III ACNGT 2 cut(s) 448, 594
HpyCH4IV ACGT 2 cut(s) 117, 808
HpyCH4V TGCA 3 cut(s) 338, 820, 1085
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 4 cut(s) 36, 233, 438, 735
HpySE526I ACGT 2 cut(s) 117, 808
Hsp92II CATG 8 cut(s) 269, 314, 335, 714, 895, 1044, 1058, 1114
KspAI GTTAAC 1 cut(s) 1062
Kzo9I GATC 3 cut(s) 427, 433, 676
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 5 cut(s) 223, 262, 549, 584, 604
Lsp1109I GCAGC 1 cut(s) 871
LweI GCATC 3 cut(s) 325, 640, 807
MaeI CTAG 2 cut(s) 222, 578
MaeII ACGT 2 cut(s) 117, 808
MaeIII GTNAC 1 cut(s) 887
MalI GATC 3 cut(s) 429, 435, 678
MboI GATC 3 cut(s) 427, 433, 676
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 3 cut(s) 206, 688, 1077
MlsI TGGCCA 2 cut(s) 287, 622
MluCI AATT 4 cut(s) 41, 365, 506, 740
MluNI TGGCCA 2 cut(s) 287, 622
MlyI GAGTC 1 cut(s) 884
MmeI TCCRAC 1 cut(s) 499
Mox20I TGGCCA 2 cut(s) 287, 622
MscI TGGCCA 2 cut(s) 287, 622
MseI TTAA 2 cut(s) 1061, 1115
Msp20I TGGCCA 2 cut(s) 287, 622
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 4 cut(s) 198, 199, 411, 624
MvaI CCWGG 2 cut(s) 411, 624
MwoI GCNNNNNNNGC 1 cut(s) 344
NciI CCSGG 2 cut(s) 198, 199
NcoI CCATGG 2 cut(s) 710, 1040
NdeII GATC 3 cut(s) 427, 433, 676
NheI GCTAGC 1 cut(s) 577
NlaIII CATG 8 cut(s) 269, 314, 335, 714, 895, 1044, 1058, 1114
NlaIV GGNNCC 5 cut(s) 193, 203, 204, 502, 964
NmuCI GTSAC 1 cut(s) 887
PciSI GCTCTTC 1 cut(s) 468
PfeI GAWTC 2 cut(s) 148, 495
PflMI CCANNNNNTGG 1 cut(s) 416
PkrI GCNGC 1 cut(s) 861
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
Ppu21I YACGTR 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 409, 622
PspFI CCCAGC 1 cut(s) 1070
PspGI CCWGG 2 cut(s) 409, 622
PspN4I GGNNCC 5 cut(s) 193, 203, 204, 502, 964
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 5 cut(s) 159, 202, 203, 500, 963
PstNI CAGNNNCTG 1 cut(s) 1070
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 3 cut(s) 387, 563, 834
RsaNI GTAC 3 cut(s) 386, 562, 833
SalI GTCGAC 1 cut(s) 643
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 2 cut(s) 1061, 1115
SatI GCNGC 1 cut(s) 860
Sau3AI GATC 3 cut(s) 427, 433, 676
Sau96I GGNCC 5 cut(s) 159, 202, 203, 500, 963
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 4 cut(s) 198, 199, 411, 624
SduI GDGCHC 3 cut(s) 206, 688, 1077
SfaNI GCATC 3 cut(s) 325, 640, 807
SfcI CTRYAG 1 cut(s) 927
SinI GGWCC 2 cut(s) 500, 963
SmaI CCCGGG 1 cut(s) 199
SnaBI TACGTA 1 cut(s) 809
Sse9I AATT 4 cut(s) 41, 365, 506, 740
SsiI CCGC 3 cut(s) 170, 189, 442
SspMI CTAG 2 cut(s) 222, 578
StyD4I CCNGG 4 cut(s) 196, 197, 409, 622
StyI CCWWGG 2 cut(s) 710, 1040
TaaI ACNGT 2 cut(s) 448, 594
TaiI ACGT 2 cut(s) 120, 811
TaqI TCGA 6 cut(s) 155, 275, 372, 644, 691, 878
TasI AATT 4 cut(s) 41, 365, 506, 740
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 2 cut(s) 148, 495
Tru1I TTAA 2 cut(s) 1061, 1115
Tru9I TTAA 2 cut(s) 1061, 1115
TscAI CASTG 3 cut(s) 345, 453, 597
TseFI GTSAC 1 cut(s) 887
TseI GCWGC 1 cut(s) 859
Tsp45I GTSAC 1 cut(s) 887
TspDTI ATGAA 5 cut(s) 102, 299, 741, 972, 1119
TspGWI ACGGA 2 cut(s) 867, 955
TspMI CCCGGG 1 cut(s) 197
TspRI CASTG 3 cut(s) 345, 453, 597
Van91I CCANNNNNTGG 1 cut(s) 416
VpaK11BI GGWCC 2 cut(s) 500, 963
XapI RAATTY 2 cut(s) 365, 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 796
XmaI CCCGGG 1 cut(s) 197
XmiI GTMKAC 2 cut(s) 644, 870
XspI CTAG 2 cut(s) 222, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.