FvH4_6g18490

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000873
Physical Location & Seq
Forward (+)
12253654 .. 12259437
5784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18490.t3

Sequence Viewer

Length: 1131 bp
ATGGCCGGTGGAGTTAACCGGAAGATATCGGCCGCTTCGGCCCGAGCTCACACCCGTAGAGCCAAGCAGAACAGCTCCTTCCGTCTTCCTTCAGGGATGTTTAGGACAACCCTAGTAGTGTTGTTTATGGGGTTTCTGGCATGGGCGTATCAGGCCATCCAACCTCCTCCACCGAAGATATGTGGTTCTCCTGATGGCCCTCCTGTTACAGCACCAAGAATAAAACTTAAGGATGGAAGGCATTTAGCCTATCAAGAGCATGGTGTCCCAAAAGAAAATGCTCAGTATAAAATTGTTTTTGTCCATGGTATCAATTCTTGCAGACATAATGCTGTTGTTGCGGAAAACTTGTCTCCAGAAATTGTTGAAGACTTGGGTATCTATATCGTATCTTTTGACAGACCAGGTTATGGAGAGAGTGATCCTGATCCAAAGAGAACAGCAAAGAGTTTGGCTCTGGATATAGAGGAGCTGGCTGATGAATTAGGATTAGGATCCAGATTCTATGTAATTGGGTTTTCCATGGGTGGACAGGCACTTTGGGGTTGCCTCAAGTACATCCCTCACAGGCTAGCAGGAGCGGCACTATTAGCGCCAGTTGTTAATTACTGGTGGACTGGTATTCCTGCAAATCTTTCTAATGAAGCATACTACAAACAGTTTCAGCAAGACCAATGGGCAGTTCGTGTTTCTCACTACACCCCTTGGCTTACCTATTTTTGGAACACTCAAAAATGGTTTCCCGCTTCAAGTGTTTTGGCTCAGAGCACAGATATTCTTTCTCAACAAGATAAAGAACTCCTCCCTAAGCTTTTCAAAGGAAAACCTAATATGGACCAGGTAAAACCGCAAGGAGATTTCGAGTCCATTCACCGTGACATCAATATTGGATTTGGGACCTGGGAGTTCGGTCCTCTGGATCTGGAAAATCCTTTTCCAAACAATGAAGCTTCTGTTCACATGTGGCAAGGTGACGACGATAACATGGTTCCTGTTACCCTGCAACGGTACATTGCCCAACAACTTCCCTGGATTCGATATCATGAGATACCAGGTGCTGGACACTTGTTCCCACATGCTGATGGATTGTGCGATACCATTGTCAAGGCACTTCTAATTGATGAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

377

Amino Acids

42.21

Weight (kDa)

6.64

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 63 - 175 2.4e-06 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 66 - 328 5e-16 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 66 - 325 5.3e-15 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 410, 1058
AccBSI CCGCTC 1 cut(s) 581
AciI CCGC 5 cut(s) 33, 341, 581, 744, 848
AclWI GGATC 5 cut(s) 416, 422, 489, 502, 927
AcoI YGGCCR 2 cut(s) 3, 30
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 2 cut(s) 557, 1010
AfiI CCNNNNNNNGG 4 cut(s) 410, 720, 1005, 1058
AflII CTTAAG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 3 cut(s) 368, 750, 817
AjnI CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 5 cut(s) 47, 75, 472, 811, 950
AluI AGCT 5 cut(s) 47, 75, 472, 811, 950
Alw21I GWGCWC 2 cut(s) 49, 770
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 5 cut(s) 416, 422, 489, 502, 927
AlwNI CAGNNNCTG 1 cut(s) 1058
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 5 cut(s) 3, 30, 39, 153, 196
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
AspLEI GCGC 1 cut(s) 595
AspS9I GGNCC 5 cut(s) 40, 197, 835, 897, 911
AsuHPI GGTGA 2 cut(s) 863, 983
AsuNHI GCTAGC 1 cut(s) 571
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 3 cut(s) 835, 897, 911
BaeI ACNNNNGTAYC 4 cut(s) 361, 394, 1086, 1119
BamHI GGATCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 49
BbsI GAAGAC 2 cut(s) 77, 375
Bbv12I GWGCWC 2 cut(s) 49, 770
BccI CCATC 4 cut(s) 164, 188, 227, 1076
BciT130I CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 2 cut(s) 113, 572
BfoI RGCGCY 1 cut(s) 596
BfrI CTTAAG 1 cut(s) 227
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 2 cut(s) 33, 582
BlsI GCNGC 2 cut(s) 34, 583
Bme1390I CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
Bme18I GGWCC 3 cut(s) 835, 897, 911
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 5 cut(s) 40, 197, 835, 897, 911
BmiI GGNNCC 3 cut(s) 496, 898, 990
BmrFI CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 2 cut(s) 77, 375
BplI GAGNNNNNCTC 2 cut(s) 439, 471
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 536
BsaBI GATNNNNATC 2 cut(s) 426, 493
BsaJI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BsaWI WCCGGW 1 cut(s) 18
Bsc4I CCNNNNNNNGG 4 cut(s) 410, 720, 1005, 1058
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 3 cut(s) 596, 614, 622
Bse3DI GCAATG 1 cut(s) 1011
Bse8I GATNNNNATC 2 cut(s) 426, 493
BseBI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BseDI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BseGI GGATG 4 cut(s) 102, 156, 238, 558
BseJI GATNNNNATC 2 cut(s) 426, 493
BseLI CCNNNNNNNGG 4 cut(s) 410, 720, 1005, 1058
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 2 cut(s) 296, 776
BseNI ACTGG 3 cut(s) 596, 614, 622
BseRI GAGGAG 3 cut(s) 156, 482, 791
BseX3I CGGCCG 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 5, 32, 41, 155, 198
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 2 cut(s) 49, 770
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 6, 19
BslFI GGGAC 2 cut(s) 251, 910
BslI CCNNNNNNNGG 4 cut(s) 410, 720, 1005, 1058
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 251, 910
BsnI GGCC 5 cut(s) 5, 32, 41, 155, 198
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 49, 770
Bsp143I GATC 4 cut(s) 421, 427, 494, 919
Bsp19I CCATGG 2 cut(s) 304, 522
BspACI CCGC 5 cut(s) 33, 341, 581, 744, 848
BspANI GGCC 5 cut(s) 5, 32, 41, 155, 198
BspCNI CTCAG 2 cut(s) 295, 775
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 3 cut(s) 496, 898, 990
BspOI GCTAGC 1 cut(s) 575
BspPI GGATC 5 cut(s) 416, 422, 489, 502, 927
BspTI CTTAAG 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 1011
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 3 cut(s) 596, 614, 622
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BssMI GATC 4 cut(s) 421, 427, 494, 919
BssT1I CCWWGG 3 cut(s) 304, 522, 704
Bst2UI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
Bst4CI ACNGT 3 cut(s) 660, 875, 1008
BstAFI CTTAAG 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 474, 573
BstDEI CTNAG 3 cut(s) 282, 762, 807
BstDSI CCRYGG 2 cut(s) 304, 522
BstF5I GGATG 4 cut(s) 102, 156, 238, 558
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 4 cut(s) 424, 430, 497, 922
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 4 cut(s) 421, 427, 494, 919
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 5 cut(s) 38, 152, 338, 581, 590
BstNI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
BstNSI RCATGY 2 cut(s) 964, 1079
BstSCI CCNGG 5 cut(s) 403, 837, 899, 1028, 1051
BstV2I GAAGAC 2 cut(s) 77, 375
BstX2I RGATCY 2 cut(s) 494, 919
BstYI RGATCY 2 cut(s) 494, 919
BstZI CGGCCG 1 cut(s) 30
BsuRI GGCC 5 cut(s) 5, 32, 41, 155, 198
BtgI CCRYGG 2 cut(s) 304, 522
BtsCI GGATG 4 cut(s) 102, 156, 238, 558
Cac8I GCNNGC 2 cut(s) 474, 573
CaiI CAGNNNCTG 1 cut(s) 1058
CciI TCATGA 1 cut(s) 1042
CfoI GCGC 1 cut(s) 595
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 5 cut(s) 40, 197, 835, 897, 911
CsiI ACCWGGT 3 cut(s) 403, 837, 1051
Csp6I GTAC 2 cut(s) 556, 1009
CviAII CATG 8 cut(s) 141, 260, 305, 523, 961, 985, 1043, 1076
CviQI GTAC 2 cut(s) 556, 1009
DdeI CTNAG 3 cut(s) 282, 762, 807
DpnI GATC 4 cut(s) 423, 429, 496, 921
DpnII GATC 4 cut(s) 421, 427, 494, 919
EaeI YGGCCR 2 cut(s) 3, 30
EagI CGGCCG 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 47
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 304, 522, 704
Eco24I GRGCYC 1 cut(s) 49
Eco32I GATATC 2 cut(s) 27, 1040
Eco47I GGWCC 3 cut(s) 835, 897, 911
Eco52I CGGCCG 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 897
EcoRII CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
EcoRV GATATC 2 cut(s) 27, 1040
EcoT14I CCWWGG 3 cut(s) 304, 522, 704
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 3 cut(s) 304, 522, 704
FaeI CATG 8 cut(s) 144, 263, 308, 526, 964, 988, 1046, 1079
FaqI GGGAC 2 cut(s) 251, 910
FatI CATG 8 cut(s) 140, 259, 304, 522, 960, 984, 1042, 1075
FauI CCCGC 1 cut(s) 751
Fnu4HI GCNGC 2 cut(s) 33, 582
FokI GGATG 4 cut(s) 109, 143, 245, 545
FriOI GRGCYC 1 cut(s) 49
Fsp4HI GCNGC 2 cut(s) 33, 582
FspBI CTAG 2 cut(s) 113, 572
GlaI GCGC 1 cut(s) 594
GluI GCNGC 2 cut(s) 33, 582
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 5 cut(s) 5, 32, 41, 155, 198
HapII CCGG 2 cut(s) 6, 19
HhaI GCGC 1 cut(s) 595
Hin1II CATG 8 cut(s) 144, 263, 308, 526, 964, 988, 1046, 1079
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 2 cut(s) 809, 948
HinfI GANTC 3 cut(s) 501, 863, 1033
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 2 cut(s) 6, 19
HphI GGTGA 2 cut(s) 863, 983
Hpy166II GTNNAC 4 cut(s) 16, 530, 615, 958
Hpy188I TCNGA 1 cut(s) 765
Hpy188III TCNNGA 9 cut(s) 191, 254, 356, 425, 458, 498, 917, 923, 1043
Hpy8I GTNNAC 4 cut(s) 16, 530, 615, 958
Hpy99I CGWCG 1 cut(s) 980
HpyAV CCTTC 3 cut(s) 88, 99, 231
HpyCH4III ACNGT 3 cut(s) 660, 875, 1008
HpyCH4V TGCA 3 cut(s) 321, 629, 1003
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 152, 338, 581, 590
HpyF3I CTNAG 3 cut(s) 282, 762, 807
Hsp92II CATG 8 cut(s) 144, 263, 308, 526, 964, 988, 1046, 1079
HspAI GCGC 1 cut(s) 593
KspAI GTTAAC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 421, 427, 494, 919
LmnI GCTCC 3 cut(s) 80, 469, 578
MabI ACCWGGT 3 cut(s) 403, 837, 1051
MaeI CTAG 2 cut(s) 113, 572
MaeIII GTNAC 4 cut(s) 205, 875, 971, 994
MalI GATC 4 cut(s) 423, 429, 496, 921
MbiI CCGCTC 1 cut(s) 581
MboI GATC 4 cut(s) 421, 427, 494, 919
MboII GAAGA 4 cut(s) 34, 77, 187, 380
MflI RGATCY 2 cut(s) 494, 919
MhlI GDGCHC 2 cut(s) 49, 770
MluCI AATT 7 cut(s) 291, 313, 360, 482, 510, 604, 1116
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 8 cut(s) 174, 177, 210, 460, 560, 573, 812, 924
MseI TTAA 3 cut(s) 15, 228, 603
MslI CAYNNNNRTG 1 cut(s) 1080
MspCI CTTAAG 1 cut(s) 227
MspI CCGG 2 cut(s) 6, 19
MspR9I CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
MvaI CCWGG 5 cut(s) 405, 839, 901, 1030, 1053
MwoI GCNNNNNNNGC 5 cut(s) 38, 152, 338, 581, 590
NcoI CCATGG 2 cut(s) 304, 522
NdeII GATC 4 cut(s) 421, 427, 494, 919
NheI GCTAGC 1 cut(s) 571
NlaIII CATG 8 cut(s) 144, 263, 308, 526, 964, 988, 1046, 1079
NlaIV GGNNCC 3 cut(s) 496, 898, 990
NmuCI GTSAC 2 cut(s) 875, 971
NspI RCATGY 2 cut(s) 964, 1079
PagI TCATGA 1 cut(s) 1042
PciI ACATGT 1 cut(s) 960
PfeI GAWTC 2 cut(s) 501, 1033
PflMI CCANNNNNTGG 2 cut(s) 410, 1058
PkrI GCNGC 2 cut(s) 34, 583
PleI GAGTC 1 cut(s) 871
PpsI GAGTC 1 cut(s) 871
PpuMI RGGWCCY 1 cut(s) 897
PscI ACATGT 1 cut(s) 960
Psp124BI GAGCTC 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 897
Psp6I CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
PspGI CCWGG 5 cut(s) 403, 837, 899, 1028, 1051
PspN4I GGNNCC 3 cut(s) 496, 898, 990
PspPI GGNCC 5 cut(s) 40, 197, 835, 897, 911
PspPPI RGGWCCY 1 cut(s) 897
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 2 cut(s) 494, 919
RsaI GTAC 2 cut(s) 557, 1010
RsaNI GTAC 2 cut(s) 556, 1009
RseI CAYNNNNRTG 1 cut(s) 1080
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 15, 228, 603
SatI GCNGC 2 cut(s) 33, 582
Sau3AI GATC 4 cut(s) 421, 427, 494, 919
Sau96I GGNCC 5 cut(s) 40, 197, 835, 897, 911
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 5 cut(s) 405, 839, 901, 1030, 1053
SduI GDGCHC 2 cut(s) 49, 770
SexAI ACCWGGT 3 cut(s) 403, 837, 1051
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SinI GGWCC 3 cut(s) 835, 897, 911
SmiMI CAYNNNNRTG 1 cut(s) 1080
SmlI CTYRAG 2 cut(s) 227, 551
SmoI CTYRAG 2 cut(s) 227, 551
Sse9I AATT 7 cut(s) 291, 313, 360, 482, 510, 604, 1116
SsiI CCGC 5 cut(s) 33, 341, 581, 744, 848
SspI AATATT 1 cut(s) 886
SspMI CTAG 2 cut(s) 113, 572
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 5 cut(s) 403, 837, 899, 1028, 1051
StyI CCWWGG 3 cut(s) 304, 522, 704
TaaI ACNGT 3 cut(s) 660, 875, 1008
TaqI TCGA 2 cut(s) 861, 1036
TaqII GACCGA 1 cut(s) 899
TasI AATT 7 cut(s) 291, 313, 360, 482, 510, 604, 1116
TatI WGTACW 1 cut(s) 555
TauI GCSGC 2 cut(s) 35, 584
TfiI GAWTC 2 cut(s) 501, 1033
Tru1I TTAA 3 cut(s) 15, 228, 603
Tru9I TTAA 3 cut(s) 15, 228, 603
TseFI GTSAC 2 cut(s) 875, 971
Tsp45I GTSAC 2 cut(s) 875, 971
TspDTI ATGAA 3 cut(s) 495, 657, 960
TspGWI ACGGA 1 cut(s) 71
Van91I CCANNNNNTGG 2 cut(s) 410, 1058
Vha464I CTTAAG 1 cut(s) 227
VpaK11BI GGWCC 3 cut(s) 835, 897, 911
XceI RCATGY 2 cut(s) 964, 1079
XspI CTAG 2 cut(s) 113, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.