Rroxscaffold_4G00318180

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000873
Physical Location & Seq
Forward (+)
44198701 .. 44202143
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318180.1

Sequence Viewer

Length: 1041 bp
ATGTTCAGGAGAGTCGCATTTTTGTTGTTGGTTGGATTGCTGGCATGGGCTTATCAGGCCACCCAACCGCCCCCTCCGAAGATTTGCGGTTCCCCCGGCGGCCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCCTACAAGGAGCACGGTGTGTCGAAACACGTGGCCAGATATAAGATTATCTTTGTTCATGGCTTTGGTTCTTCCAGACATGATGCAGTGATTGCGACCAAAACTTCACCGGAAGTTATTGAAGAGTTAGGGGTACACATTGTGTCTTTTGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGTTCCAAATTTTATGTAGTTGGATATTCTATGGGTGGACAGGTTATCTGGAGCTGTCTTCAGTACATCCCTCATAGGCTAGCTGGAGCTACACTGTATGCTCCTGTTATCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCGACAGATGCCTACTATAAACAACCCCTCGGGGATCAATGGGCACTTCGAGTTGCTCACCATATACCATGGCTAACCTACTGGTGGAACACTCAGAAATTATTTCCTTCATCAAGCGTTATAGTTGGTAAACCCGAGATTTTCTCCAGTCAGGACTTGGAAATACGTAGGAAGATGCAGGCTTCTGGCGTACAAACTAACAGGCCACACGGAAAGCAGCAAGGAGTGTACGAGTCGAACCATCGTGACATGATGATTGGATTTGGGAGATGGGAGTTTGACCCTATAGAAATCAAGAACCCATTTCCCAACAATGAAGGGTCCGTCCATCTGTGGCAGGGTGATGAAGATAGGCTTGTCCCAGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTGACTACCATGAGTTACCAGGTGCTGGGCACTTGTTTGCACATAACGATGGTATAAATGAAGCTATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

39.12

Weight (kDa)

8.35

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 63 - 325 1.8e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 327 4.7e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 328 1.8e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 314, 968
AccI GTMKAC 1 cut(s) 542
AciI CCGC 3 cut(s) 68, 87, 99
AclWI GGATC 3 cut(s) 320, 326, 582
AcoI YGGCCR 2 cut(s) 183, 518
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 440
AcvI CACGTG 1 cut(s) 181
AdeI CACNNNGTG 2 cut(s) 169, 292
AfaI GTAC 4 cut(s) 285, 461, 732, 770
AfiI CCNNNNNNNGG 3 cut(s) 314, 624, 968
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 3 cut(s) 272, 908, 914
AjnI CCWGG 3 cut(s) 307, 520, 961
AluBI AGCT 9 cut(s) 116, 130, 376, 388, 450, 479, 485, 934, 1007
AluI AGCT 9 cut(s) 116, 130, 376, 388, 450, 479, 485, 934, 1007
Alw21I GWGCWC 1 cut(s) 165
AlwI GGATC 3 cut(s) 320, 326, 582
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 2 cut(s) 569, 674
AoxI GGCC 6 cut(s) 57, 100, 150, 183, 518, 743
ApeKI GCWGC 1 cut(s) 757
ApoI RAATTY 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 101, 861
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 4 cut(s) 249, 329, 590, 893
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 2 cut(s) 569, 674
AvaII GGWCC 1 cut(s) 861
BaeGI GKGCMC 2 cut(s) 586, 975
BalI TGGCCA 2 cut(s) 185, 520
BbrPI CACGTG 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 769
BccI CCATC 6 cut(s) 116, 131, 789, 804, 876, 986
BciT130I CCWGG 3 cut(s) 309, 522, 963
BcnI CCSGG 1 cut(s) 96
BfaI CTAG 2 cut(s) 120, 476
BfmI CTRYAG 1 cut(s) 825
BisI GCNGC 2 cut(s) 100, 758
BlsI GCNGC 2 cut(s) 101, 759
Bme1390I CCNGG 4 cut(s) 96, 309, 522, 963
Bme18I GGWCC 1 cut(s) 861
BmeT110I CYCGRG 2 cut(s) 569, 674
BmgT120I GGNCC 2 cut(s) 101, 861
BmiI GGNNCC 3 cut(s) 91, 400, 862
BmrFI CCNGG 4 cut(s) 96, 309, 522, 963
BmrI ACTGGG 2 cut(s) 896, 1038
BmsI GCATC 3 cut(s) 223, 538, 705
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 2 cut(s) 896, 1038
BpiI GAAGAC 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 3 cut(s) 466, 501, 670
Bpu10I CCTNAGC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 96
BsaAI YACGTR 2 cut(s) 181, 707
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 4 cut(s) 94, 568, 608, 938
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 624, 968
Bse1I ACTGG 5 cut(s) 518, 626, 687, 902, 1033
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 3 cut(s) 309, 522, 963
BseDI CCNNGG 4 cut(s) 94, 568, 608, 938
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 314, 624, 968
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BseSI GKGCMC 2 cut(s) 586, 975
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 968
BshFI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 2 cut(s) 569, 674
BsiSI CCGG 2 cut(s) 96, 260
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 3 cut(s) 314, 624, 968
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BsoBI CYCGRG 2 cut(s) 569, 674
Bsp1286I GDGCHC 3 cut(s) 165, 586, 975
Bsp143I GATC 3 cut(s) 325, 331, 574
Bsp19I CCATGG 2 cut(s) 608, 938
BspACI CCGC 3 cut(s) 68, 87, 99
BspANI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BspCNI CTCAG 1 cut(s) 646
BspLI GGNNCC 3 cut(s) 91, 400, 862
BspOI GCTAGC 1 cut(s) 479
BspPI GGATC 3 cut(s) 320, 326, 582
BspQI GCTCTTC 1 cut(s) 366
BsrI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BssECI CCNNGG 4 cut(s) 94, 568, 608, 938
BssMI GATC 3 cut(s) 325, 331, 574
BssT1I CCWWGG 2 cut(s) 608, 938
Bst2UI CCWGG 3 cut(s) 309, 522, 963
Bst4CI ACNGT 3 cut(s) 167, 346, 492
Bst6I CTCTTC 3 cut(s) 267, 344, 366
BstAPI GCANNNNNTGC 1 cut(s) 242
BstBAI YACGTR 2 cut(s) 181, 707
BstC8I GCNNGC 4 cut(s) 42, 378, 477, 720
BstDEI CTNAG 3 cut(s) 131, 336, 633
BstDSI CCRYGG 2 cut(s) 608, 938
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 3 cut(s) 328, 334, 577
BstMBI GATC 3 cut(s) 325, 331, 574
BstMWI GCNNNNNNNGC 2 cut(s) 56, 242
BstNI CCWGG 3 cut(s) 309, 522, 963
BstSCI CCNGG 4 cut(s) 94, 307, 520, 961
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 2 cut(s) 586, 975
BstSNI TACGTA 1 cut(s) 707
BstV1I GCAGC 1 cut(s) 769
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BtgI CCRYGG 2 cut(s) 608, 938
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 351, 488
Cac8I GCNNGC 4 cut(s) 42, 378, 477, 720
CaiI CAGNNNCTG 1 cut(s) 968
Cfr13I GGNCC 2 cut(s) 101, 861
CsiI ACCWGGT 1 cut(s) 961
Csp6I GTAC 4 cut(s) 284, 460, 731, 769
CviAII CATG 7 cut(s) 45, 209, 230, 609, 790, 939, 953
CviQI GTAC 4 cut(s) 284, 460, 731, 769
DdeI CTNAG 3 cut(s) 131, 336, 633
DpnI GATC 3 cut(s) 327, 333, 576
DpnII GATC 3 cut(s) 325, 331, 574
DraIII CACNNNGTG 2 cut(s) 169, 292
EaeI YGGCCR 2 cut(s) 183, 518
Eam1104I CTCTTC 3 cut(s) 267, 344, 366
EarI CTCTTC 3 cut(s) 267, 344, 366
Eco105I TACGTA 1 cut(s) 707
Eco130I CCWWGG 2 cut(s) 608, 938
Eco47I GGWCC 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 440
Eco72I CACGTG 1 cut(s) 181
Eco88I CYCGRG 2 cut(s) 569, 674
EcoRII CCWGG 3 cut(s) 307, 520, 961
EcoT14I CCWWGG 2 cut(s) 608, 938
ErhI CCWWGG 2 cut(s) 608, 938
FaeI CATG 7 cut(s) 48, 212, 233, 612, 793, 942, 956
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 879, 911
FaqI GGGAC 1 cut(s) 884
FatI CATG 7 cut(s) 44, 208, 229, 608, 789, 938, 952
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 100, 758
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 100, 758
FspBI CTAG 2 cut(s) 120, 476
GluI GCNGC 2 cut(s) 100, 758
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 3 cut(s) 466, 501, 670
HaeIII GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
HapII CCGG 2 cut(s) 96, 260
Hin1II CATG 7 cut(s) 48, 212, 233, 612, 793, 942, 956
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 3 cut(s) 12, 393, 773
HpaII CCGG 2 cut(s) 96, 260
HphI GGTGA 4 cut(s) 249, 329, 590, 893
Hpy166II GTNNAC 5 cut(s) 286, 434, 543, 671, 769
Hpy188I TCNGA 3 cut(s) 78, 392, 636
Hpy188III TCNNGA 7 cut(s) 7, 225, 329, 445, 692, 785, 835
Hpy8I GTNNAC 5 cut(s) 286, 434, 543, 671, 769
HpyAV CCTTC 2 cut(s) 657, 851
HpyCH4III ACNGT 3 cut(s) 167, 346, 492
HpyCH4IV ACGT 2 cut(s) 180, 706
HpyCH4V TGCA 3 cut(s) 236, 718, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 242
HpyF3I CTNAG 3 cut(s) 131, 336, 633
HpySE526I ACGT 2 cut(s) 180, 706
Hsp92II CATG 7 cut(s) 48, 212, 233, 612, 793, 942, 956
Kzo9I GATC 3 cut(s) 325, 331, 574
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 5 cut(s) 121, 160, 447, 482, 502
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 3 cut(s) 223, 538, 705
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 2 cut(s) 120, 476
MaeII ACGT 2 cut(s) 180, 706
MaeIII GTNAC 2 cut(s) 785, 957
MalI GATC 3 cut(s) 327, 333, 576
MboI GATC 3 cut(s) 325, 331, 574
MboII GAAGA 9 cut(s) 91, 153, 213, 284, 361, 383, 446, 724, 899
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 3 cut(s) 165, 586, 975
MlsI TGGCCA 2 cut(s) 185, 520
MluCI AATT 2 cut(s) 404, 638
MluNI TGGCCA 2 cut(s) 185, 520
MlyI GAGTC 2 cut(s) 21, 782
MmeI TCCRAC 2 cut(s) 13, 397
MnlI CCTC 3 cut(s) 84, 477, 578
Mox20I TGGCCA 2 cut(s) 185, 520
MscI TGGCCA 2 cut(s) 185, 520
MseI TTAA 1 cut(s) 1013
MslI CAYNNNNRTG 1 cut(s) 990
Msp20I TGGCCA 2 cut(s) 185, 520
MspI CCGG 2 cut(s) 96, 260
MspR9I CCNGG 4 cut(s) 96, 309, 522, 963
MvaI CCWGG 3 cut(s) 309, 522, 963
MwoI GCNNNNNNNGC 2 cut(s) 56, 242
NciI CCSGG 1 cut(s) 96
NcoI CCATGG 2 cut(s) 608, 938
NdeII GATC 3 cut(s) 325, 331, 574
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 7 cut(s) 48, 212, 233, 612, 793, 942, 956
NlaIV GGNNCC 3 cut(s) 91, 400, 862
NmuCI GTSAC 1 cut(s) 785
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 393
PflMI CCANNNNNTGG 2 cut(s) 314, 968
PkrI GCNGC 2 cut(s) 101, 759
PleI GAGTC 2 cut(s) 20, 781
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 2 cut(s) 20, 781
Ppu21I YACGTR 2 cut(s) 181, 707
Psp6I CCWGG 3 cut(s) 307, 520, 961
PspCI CACGTG 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 3 cut(s) 307, 520, 961
PspN4I GGNNCC 3 cut(s) 91, 400, 862
PspPI GGNCC 2 cut(s) 101, 861
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 4 cut(s) 285, 461, 732, 770
RsaNI GTAC 4 cut(s) 284, 460, 731, 769
RseI CAYNNNNRTG 1 cut(s) 990
SalI GTCGAC 1 cut(s) 541
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 1013
SatI GCNGC 2 cut(s) 100, 758
Sau3AI GATC 3 cut(s) 325, 331, 574
Sau96I GGNCC 2 cut(s) 101, 861
SchI GAGTC 2 cut(s) 21, 782
ScrFI CCNGG 4 cut(s) 96, 309, 522, 963
SduI GDGCHC 3 cut(s) 165, 586, 975
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 3 cut(s) 223, 538, 705
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 990
SnaBI TACGTA 1 cut(s) 707
Sse9I AATT 2 cut(s) 404, 638
SsiI CCGC 3 cut(s) 68, 87, 99
SspMI CTAG 2 cut(s) 120, 476
StyD4I CCNGG 4 cut(s) 94, 307, 520, 961
StyI CCWWGG 2 cut(s) 608, 938
TaaI ACNGT 3 cut(s) 167, 346, 492
TaiI ACGT 2 cut(s) 183, 709
TaqI TCGA 4 cut(s) 173, 542, 589, 776
TasI AATT 2 cut(s) 404, 638
TatI WGTACW 1 cut(s) 459
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 1 cut(s) 1013
Tru9I TTAA 1 cut(s) 1013
TscAI CASTG 3 cut(s) 243, 351, 495
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 1 cut(s) 757
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 5 cut(s) 197, 639, 870, 900, 1017
TspGWI ACGGA 2 cut(s) 765, 853
TspRI CASTG 3 cut(s) 243, 351, 495
Van91I CCANNNNNTGG 2 cut(s) 314, 968
VpaK11BI GGWCC 1 cut(s) 861
XapI RAATTY 1 cut(s) 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 2 cut(s) 120, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.