pycom12g02260

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000873
Physical Location & Seq
Forward (+)
1974144 .. 1977773
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02260.2

Sequence Viewer

Length: 1128 bp
ATGGCGGGAGGAGTGAACAGGAAGATATCAGCTGCGTCGGCGAGAGCTCACACCCGAAGAGCCAAGCAAAACAACTCCTTCCAGCTTCCGGCGGGGATTTTTAAGACAGCACTAGTAGCGTTGGTTATTGGACTTTCGGCATGGGCATATCAGGCAATCCAACCTCCTCCATCAAAGATATGTGGCTCTCCTGATGGGCCTCCTGTTACAGGGCCAAGAATAAAACTTAGGGATGGAAGGCATTTAGCTTACCAAGAGCATGGAGTCCCAAAAGATAATGCGCAGCATAAAATAGTTTATCTCCATGGTATTGATAGTTGCAGGCATGATGCTGTCATTCCCAACTTCCTGTCTCCGGAAACCGTGAAAGACTTGGGGATCTACGTTGTGTCTTTTGACAGACCTGGTTATGGAGAAAGTGATCCTAATCCAAAGCGAACGGTGAAGAGTATGGCTACAGATATAGAGGAGCTGGCTGATCAATTGGGTTTAGGACACAGATTTTATGTAATTGGTTTCTCCATGGGTGGACAGGTGATTTGGAGCTGCCTCAAATATATCCCTCACCGGATTGCAGGAGCAGCACTAGTAGCTCCAGTGGTAAACTACTGGTGGACTGGTATTCCTTCAAATCTATCTAGTCAAGCTTACTCCCAACAGCTTCAGCCAGACCAGTGGGCAGTTCGTGTGTCTCACTACACCCCTTGGCTTACGTACTTCTGGAACACTCAAAAATGGTTTCCTGGTTCAAGTGTTCTGGCTCAAAGTATAGATTGTCTTTCTGACCAAGACAAAGAAATCCTGCCTAAGCTTCAAAAAAGAGAACCATATGCGGGACAGGTACGACAGCAAGGAGAATTTGAGTCCATTTACCGTGACATGAATGTTGGATTTGGGACCTGGGAATTCAGTCCTCTGGAACTGGAAAACCCATTCCCTAACAATGAAGCTTCTGTCCACGTGTGGCATGGAGATGAAGATCGCATGGTACCCGTTTTACTGCAACGCTACATTGCTGAACAGCTTCCATGGATCCACTATCATGAGATACCAGGTGCTGGACTTTTGTTGCCACTTGCTGATGGGATGTGCGAGACCATTGTCAAGGCACTTTTAACCGACGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

376

Amino Acids

41.86

Weight (kDa)

6.67

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 282
Acc65I GGTACC 1 cut(s) 988
AccB1I GGYRCC 1 cut(s) 988
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 3 cut(s) 5, 92, 833
AclWI GGATC 4 cut(s) 386, 416, 1027, 1040
AcsI RAATTY 2 cut(s) 857, 905
AcuI CTGAAG 1 cut(s) 647
AcvI CACGTG 1 cut(s) 961
AfaI GTAC 3 cut(s) 716, 843, 990
AfiI CCNNNNNNNGG 6 cut(s) 88, 209, 355, 410, 833, 1058
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 3 cut(s) 630, 750, 815
AhlI ACTAGT 2 cut(s) 112, 586
AjnI CCWGG 4 cut(s) 403, 742, 899, 1051
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 3 cut(s) 357, 696, 1088
AlwI GGATC 4 cut(s) 386, 416, 1027, 1040
AlwNI CAGNNNCTG 1 cut(s) 1058
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 2 cut(s) 197, 212
ApeKI GCWGC 4 cut(s) 32, 283, 546, 581
ApoI RAATTY 2 cut(s) 857, 905
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
Asp700I GAANNNNTTC 1 cut(s) 1023
Asp718I GGTACC 1 cut(s) 988
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 3 cut(s) 197, 212, 897
AsuHPI GGTGA 3 cut(s) 454, 547, 557
AvaII GGWCC 1 cut(s) 897
BamHI GGATCC 1 cut(s) 1032
BanI GGYRCC 1 cut(s) 988
BanII GRGCYC 1 cut(s) 49
BbrPI CACGTG 1 cut(s) 961
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 4 cut(s) 19, 295, 533, 593
BccI CCATC 4 cut(s) 178, 188, 227, 1076
BciT130I CCWGG 4 cut(s) 405, 744, 901, 1053
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 3 cut(s) 357, 696, 1088
BcuI ACTAGT 2 cut(s) 112, 586
BfaI CTAG 3 cut(s) 113, 587, 639
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 4 cut(s) 33, 284, 547, 582
BlsI GCNGC 4 cut(s) 34, 285, 548, 583
Bme1390I CCNGG 4 cut(s) 405, 744, 901, 1053
Bme18I GGWCC 1 cut(s) 897
BmgT120I GGNCC 3 cut(s) 197, 212, 897
BmiI GGNNCC 3 cut(s) 898, 990, 1034
BmrFI CCNGG 4 cut(s) 405, 744, 901, 1053
BmsI GCATC 1 cut(s) 319
BoxI GACNNNNGTC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 807
BsaAI YACGTR 2 cut(s) 714, 961
BsaBI GATNNNNATC 2 cut(s) 426, 978
BsaI GGTCTC 1 cut(s) 1088
BsaJI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BsaWI WCCGGW 2 cut(s) 355, 567
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 209, 355, 410, 833, 1058
Bse1I ACTGG 5 cut(s) 596, 614, 622, 673, 927
Bse3DI GCAATG 1 cut(s) 1011
Bse8I GATNNNNATC 2 cut(s) 426, 978
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 4 cut(s) 405, 744, 901, 1053
BseDI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BseGI GGATG 2 cut(s) 238, 1092
BseJI GATNNNNATC 2 cut(s) 426, 978
BseLI CCNNNNNNNGG 6 cut(s) 88, 209, 355, 410, 833, 1058
BseMI GCAATG 1 cut(s) 1011
BseNI ACTGG 5 cut(s) 596, 614, 622, 673, 927
BseRI GAGGAG 3 cut(s) 24, 156, 482
BseXI GCAGC 4 cut(s) 19, 295, 533, 593
BshFI GGCC 2 cut(s) 199, 214
BshNI GGYRCC 1 cut(s) 988
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 3 cut(s) 89, 356, 568
BslFI GGGAC 3 cut(s) 251, 849, 910
BslI CCNNNNNNNGG 6 cut(s) 88, 209, 355, 410, 833, 1058
BsmAI GTCTC 3 cut(s) 357, 696, 1088
BsmFI GGGAC 3 cut(s) 251, 849, 910
BsnI GGCC 2 cut(s) 199, 214
Bso31I GGTCTC 1 cut(s) 1088
Bsp1286I GDGCHC 1 cut(s) 49
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 5 cut(s) 378, 421, 478, 979, 1032
Bsp19I CCATGG 3 cut(s) 304, 522, 1028
BspACI CCGC 3 cut(s) 5, 92, 833
BspANI GGCC 2 cut(s) 199, 214
BspEI TCCGGA 1 cut(s) 355
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 3 cut(s) 898, 990, 1034
BspPI GGATC 4 cut(s) 386, 416, 1027, 1040
BspQI GCTCTTC 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 988
BspTNI GGTCTC 1 cut(s) 1088
BsrDI GCAATG 1 cut(s) 1011
BsrI ACTGG 5 cut(s) 596, 614, 622, 673, 927
BssECI CCNNGG 5 cut(s) 304, 522, 704, 900, 1028
BssMI GATC 5 cut(s) 378, 421, 478, 979, 1032
BssT1I CCWWGG 4 cut(s) 304, 522, 704, 1028
Bst2UI CCWGG 4 cut(s) 405, 744, 901, 1053
Bst4CI ACNGT 3 cut(s) 364, 442, 875
Bst6I CTCTTC 2 cut(s) 52, 440
BstBAI YACGTR 2 cut(s) 714, 961
BstC8I GCNNGC 2 cut(s) 323, 474
BstDEI CTNAG 2 cut(s) 227, 807
BstDSI CCRYGG 3 cut(s) 304, 522, 1028
BstENI CCTNNNNNAGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 238, 1092
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 5 cut(s) 381, 424, 481, 982, 1035
BstMAI GTCTC 3 cut(s) 357, 696, 1088
BstMBI GATC 5 cut(s) 378, 421, 478, 979, 1032
BstMWI GCNNNNNNNGC 5 cut(s) 38, 116, 152, 581, 590
BstNI CCWGG 4 cut(s) 405, 744, 901, 1053
BstPAI GACNNNNGTC 1 cut(s) 1100
BstSCI CCNGG 4 cut(s) 403, 742, 899, 1051
BstSFI CTRYAG 1 cut(s) 456
BstSNI TACGTA 1 cut(s) 714
BstV1I GCAGC 4 cut(s) 19, 295, 533, 593
BstX2I RGATCY 2 cut(s) 378, 1032
BstXI CCANNNNNNTGG 2 cut(s) 260, 675
BstYI RGATCY 2 cut(s) 378, 1032
BsuRI GGCC 2 cut(s) 199, 214
BtgI CCRYGG 3 cut(s) 304, 522, 1028
BtsCI GGATG 2 cut(s) 238, 1092
BtsIMutI CAGTG 2 cut(s) 603, 680
Cac8I GCNNGC 2 cut(s) 323, 474
CaiI CAGNNNCTG 1 cut(s) 1058
CciI TCATGA 1 cut(s) 1042
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 3 cut(s) 197, 212, 897
CseI GACGC 1 cut(s) 24
CsiI ACCWGGT 2 cut(s) 403, 1051
Csp6I GTAC 3 cut(s) 715, 842, 989
CviQI GTAC 3 cut(s) 715, 842, 989
DdeI CTNAG 2 cut(s) 227, 807
DpnI GATC 5 cut(s) 380, 423, 480, 981, 1034
DpnII GATC 5 cut(s) 378, 421, 478, 979, 1032
Eam1104I CTCTTC 2 cut(s) 52, 440
EarI CTCTTC 2 cut(s) 52, 440
Ecl136II GAGCTC 1 cut(s) 47
Eco105I TACGTA 1 cut(s) 714
Eco130I CCWWGG 4 cut(s) 304, 522, 704, 1028
Eco24I GRGCYC 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 1088
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 897
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 647
Eco72I CACGTG 1 cut(s) 961
EcoICRI GAGCTC 1 cut(s) 47
EcoNI CCTNNNNNAGG 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 897
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 4 cut(s) 403, 742, 899, 1051
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 4 cut(s) 304, 522, 704, 1028
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 4 cut(s) 304, 522, 704, 1028
FaqI GGGAC 3 cut(s) 251, 849, 910
FauI CCCGC 2 cut(s) 85, 826
FauNDI CATATG 1 cut(s) 829
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 4 cut(s) 33, 284, 547, 582
FokI GGATG 2 cut(s) 245, 1099
FriOI GRGCYC 1 cut(s) 49
Fsp4HI GCNGC 4 cut(s) 33, 284, 547, 582
FspBI CTAG 3 cut(s) 113, 587, 639
FspI TGCGCA 1 cut(s) 282
GlaI GCGC 1 cut(s) 282
GluI GCNGC 4 cut(s) 33, 284, 547, 582
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 2 cut(s) 199, 214
HapII CCGG 3 cut(s) 89, 356, 568
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 283
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HindIII AAGCTT 3 cut(s) 645, 809, 948
HinfI GANTC 2 cut(s) 264, 863
HpaII CCGG 3 cut(s) 89, 356, 568
HphI GGTGA 3 cut(s) 454, 547, 557
Hpy166II GTNNAC 5 cut(s) 16, 530, 604, 615, 958
Hpy188I TCNGA 1 cut(s) 784
Hpy188III TCNNGA 5 cut(s) 191, 356, 721, 917, 1043
Hpy8I GTNNAC 5 cut(s) 16, 530, 604, 615, 958
Hpy99I CGWCG 2 cut(s) 40, 1124
HpyAV CCTTC 3 cut(s) 88, 231, 636
HpyCH4III ACNGT 3 cut(s) 364, 442, 875
HpyCH4IV ACGT 3 cut(s) 384, 713, 960
HpyCH4V TGCA 3 cut(s) 321, 575, 1003
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 116, 152, 581, 590
HpyF3I CTNAG 2 cut(s) 227, 807
HpySE526I ACGT 3 cut(s) 384, 713, 960
HspAI GCGC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 355
KpnI GGTACC 1 cut(s) 992
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 5 cut(s) 378, 421, 478, 979, 1032
LguI GCTCTTC 1 cut(s) 52
LmnI GCTCC 4 cut(s) 469, 543, 578, 598
Lsp1109I GCAGC 4 cut(s) 19, 295, 533, 593
LweI GCATC 1 cut(s) 319
MabI ACCWGGT 2 cut(s) 403, 1051
MaeI CTAG 3 cut(s) 113, 587, 639
MaeII ACGT 3 cut(s) 384, 713, 960
MaeIII GTNAC 2 cut(s) 205, 875
MalI GATC 5 cut(s) 380, 423, 480, 981, 1034
MboI GATC 5 cut(s) 378, 421, 478, 979, 1032
MboII GAAGA 4 cut(s) 34, 69, 457, 989
MfeI CAATTG 1 cut(s) 482
MflI RGATCY 2 cut(s) 378, 1032
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 4 cut(s) 482, 510, 857, 905
MlyI GAGTC 2 cut(s) 273, 872
MmeI TCCRAC 2 cut(s) 184, 868
MnlI CCTC 7 cut(s) 174, 177, 210, 460, 560, 573, 924
MroI TCCGGA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 1023
MseI TTAA 2 cut(s) 102, 1115
MslI CAYNNNNRTG 2 cut(s) 972, 1041
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 89, 356, 568
MspR9I CCNGG 4 cut(s) 405, 744, 901, 1053
MunI CAATTG 1 cut(s) 482
MvaI CCWGG 4 cut(s) 405, 744, 901, 1053
MwoI GCNNNNNNNGC 5 cut(s) 38, 116, 152, 581, 590
NcoI CCATGG 3 cut(s) 304, 522, 1028
NdeI CATATG 1 cut(s) 829
NdeII GATC 5 cut(s) 378, 421, 478, 979, 1032
NlaIV GGNNCC 3 cut(s) 898, 990, 1034
NmuCI GTSAC 1 cut(s) 875
NsbI TGCGCA 1 cut(s) 282
PagI TCATGA 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 1023
PflMI CCANNNNNTGG 1 cut(s) 1058
PkrI GCNGC 4 cut(s) 34, 285, 548, 583
PleI GAGTC 2 cut(s) 272, 871
PmaCI CACGTG 1 cut(s) 961
PmlI CACGTG 1 cut(s) 961
PpsI GAGTC 2 cut(s) 272, 871
Ppu21I YACGTR 2 cut(s) 714, 961
PpuMI RGGWCCY 1 cut(s) 897
PshAI GACNNNNGTC 1 cut(s) 1100
Psp124BI GAGCTC 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 897
Psp6I CCWGG 4 cut(s) 403, 742, 899, 1051
PspCI CACGTG 1 cut(s) 961
PspGI CCWGG 4 cut(s) 403, 742, 899, 1051
PspN4I GGNNCC 3 cut(s) 898, 990, 1034
PspPI GGNCC 3 cut(s) 197, 212, 897
PspPPI RGGWCCY 1 cut(s) 897
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 2 cut(s) 378, 1032
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 716, 843, 990
RsaNI GTAC 3 cut(s) 715, 842, 989
RseI CAYNNNNRTG 2 cut(s) 972, 1041
SacI GAGCTC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 2 cut(s) 102, 1115
SatI GCNGC 4 cut(s) 33, 284, 547, 582
Sau3AI GATC 5 cut(s) 378, 421, 478, 979, 1032
Sau96I GGNCC 3 cut(s) 197, 212, 897
SchI GAGTC 2 cut(s) 273, 872
ScrFI CCNGG 4 cut(s) 405, 744, 901, 1053
SduI GDGCHC 1 cut(s) 49
SexAI ACCWGGT 2 cut(s) 403, 1051
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 897
SmiMI CAYNNNNRTG 2 cut(s) 972, 1041
SnaBI TACGTA 1 cut(s) 714
SpeI ACTAGT 2 cut(s) 112, 586
Sse9I AATT 4 cut(s) 482, 510, 857, 905
SsiI CCGC 3 cut(s) 5, 92, 833
SspMI CTAG 3 cut(s) 113, 587, 639
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 4 cut(s) 403, 742, 899, 1051
StyI CCWWGG 4 cut(s) 304, 522, 704, 1028
TaaI ACNGT 3 cut(s) 364, 442, 875
TaiI ACGT 3 cut(s) 387, 716, 963
TasI AATT 4 cut(s) 482, 510, 857, 905
Tru1I TTAA 2 cut(s) 102, 1115
Tru9I TTAA 2 cut(s) 102, 1115
TscAI CASTG 2 cut(s) 603, 680
TseFI GTSAC 1 cut(s) 875
TseI GCWGC 4 cut(s) 32, 283, 546, 581
Tsp45I GTSAC 1 cut(s) 875
TspDTI ATGAA 3 cut(s) 896, 960, 990
TspRI CASTG 2 cut(s) 603, 680
Van91I CCANNNNNTGG 1 cut(s) 1058
VpaK11BI GGWCC 1 cut(s) 897
XagI CCTNNNNNAGG 1 cut(s) 207
XapI RAATTY 2 cut(s) 857, 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 965
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 3 cut(s) 113, 587, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.