Rw1G010700

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000873
Physical Location & Seq
Forward (+)
24124884 .. 24127352
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010700.1

Sequence Viewer

Length: 1041 bp
ATGTTTAGGAGAGTCGCATTTTTGTTGTTGGTTGGATTGCTGGCATGGGCTTATCAGGCCACCCAACCGCCCCCTCCGAAGATTTGCGGTTCCCCCGGGGGCCCACCCATCACAGCTCCTAGAATAAAGCTAAGAGATGGAAGATATTTGGCCTACAAGGAGCATGGTGTGTCGAAACAAGTGGCCAGATATAAGATTATCTTTGTTCATGGCTTCGGTTCTTCCAGACATGATGCAGTGATTGCGACCAAACCTTCACCAGAATTTATCGAAGAGTTAGGGGTACACATTGTGTCTTTGGACAGACCAGGCTATGGTGAAAGTGATCCAGATCCTAAGCGAACAGTGAAGAGTTTGGCTTTAGATATAGAAGAGCTTGCTGACCAGCTCGGATTCGGATCCAAATTTTATATAGTTGGATATTCTATGGGCGGACAGGTTATCTGGAGCTGTCTTCAGTACATCCCTCATAGGCTAGCTGGAGCTACACTGTATGCTCCTGTTGTCAACTACTGGTGGCCAGGCTTTCCTGCCAACTTGTCGACAGATGCCTACAATAAACAACCCCCTGGGGATCAATGGGCACTTCGAGTTGCTCACCATATACCATGGCTAACATACTGGTGGAACAGTCAGAAATTATTTCCTTCATCAAGCGTTATAGTTGGTAAACCAGAGATTTTCTCCAGTCAGGACTTGGAAATACTTAGGAAGATGCAGGCTTCTGGCATACAAACTAACAGGCCACACGGAAAGCAGCAAGGAGTATACGAGTCGAACCATCGTGACCTGATGATTGGATTTGGGAGTTGGGAGTTTGACCCTGTAGAAATCAAGAATCCATTTCCCAACAATGAAGGGTCCGTCCATCTGTGGCAGGGTGATGAAGATAGACTTGTCCCAGTTGAACTTCAACGCTTTATTGTGGGAAAGCTTCCATGGATTGACTACCATGAGTTAACAGGTGCTGGGCACTTGTTTGCATATAACGATGGTATAAATGAAGCCATGTTAAAGTCAATGCTGACTGGGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

38.94

Weight (kDa)

7.21

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 63 - 175 1.4e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 327 1.5e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 328 5.3e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 2 cut(s) 542, 768
AciI CCGC 3 cut(s) 68, 87, 432
AclWI GGATC 5 cut(s) 320, 326, 393, 406, 582
AcoI YGGCCR 2 cut(s) 183, 518
AcsI RAATTY 2 cut(s) 263, 404
AcuI CTGAAG 1 cut(s) 440
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 2 cut(s) 285, 461
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 2 cut(s) 908, 914
AjnI CCWGG 3 cut(s) 307, 520, 568
AluBI AGCT 8 cut(s) 116, 130, 376, 388, 450, 479, 485, 934
AluI AGCT 8 cut(s) 116, 130, 376, 388, 450, 479, 485, 934
AlwI GGATC 5 cut(s) 320, 326, 393, 406, 582
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 6 cut(s) 57, 100, 150, 183, 518, 743
ApaI GGGCCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 757
ApoI RAATTY 2 cut(s) 263, 404
AspS9I GGNCC 3 cut(s) 100, 101, 861
AsuC2I CCSGG 2 cut(s) 96, 97
AsuHPI GGTGA 4 cut(s) 249, 329, 590, 893
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 861
BaeGI GKGCMC 3 cut(s) 104, 586, 975
BalI TGGCCA 2 cut(s) 185, 520
BamHI GGATCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 769
BccI CCATC 5 cut(s) 116, 131, 789, 876, 986
BciT130I CCWGG 3 cut(s) 309, 522, 570
BcnI CCSGG 2 cut(s) 96, 97
BfaI CTAG 2 cut(s) 120, 476
BfmI CTRYAG 1 cut(s) 825
BisI GCNGC 1 cut(s) 758
BlsI GCNGC 1 cut(s) 759
Bme1390I CCNGG 5 cut(s) 96, 97, 309, 522, 570
Bme18I GGWCC 1 cut(s) 861
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 3 cut(s) 100, 101, 861
BmiI GGNNCC 5 cut(s) 91, 101, 102, 400, 862
BmrFI CCNGG 5 cut(s) 96, 97, 309, 522, 570
BmrI ACTGGG 2 cut(s) 896, 1038
BmsI GCATC 3 cut(s) 223, 538, 705
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 2 cut(s) 896, 1038
BpiI GAAGAC 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 3 cut(s) 466, 501, 670
Bpu10I CCTNAGC 1 cut(s) 336
BpuMI CCSGG 2 cut(s) 96, 97
BsaBI GATNNNNATC 2 cut(s) 330, 397
BsaJI CCNNGG 7 cut(s) 94, 95, 96, 568, 569, 608, 938
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 5 cut(s) 518, 626, 687, 902, 1033
Bse8I GATNNNNATC 2 cut(s) 330, 397
BseBI CCWGG 3 cut(s) 309, 522, 570
BseDI CCNNGG 7 cut(s) 94, 95, 96, 568, 569, 608, 938
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 2 cut(s) 330, 397
BseLI CCNNNNNNNGG 1 cut(s) 314
BseNI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BseSI GKGCMC 3 cut(s) 104, 586, 975
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 968
BshFI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BsoBI CYCGRG 1 cut(s) 95
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 104, 586, 975
Bsp143I GATC 4 cut(s) 325, 331, 398, 574
Bsp19I CCATGG 2 cut(s) 608, 938
BspACI CCGC 3 cut(s) 68, 87, 432
BspANI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BspLI GGNNCC 5 cut(s) 91, 101, 102, 400, 862
BspOI GCTAGC 1 cut(s) 479
BspPI GGATC 5 cut(s) 320, 326, 393, 406, 582
BspQI GCTCTTC 1 cut(s) 366
BsrI ACTGG 5 cut(s) 518, 626, 687, 902, 1033
BssECI CCNNGG 7 cut(s) 94, 95, 96, 568, 569, 608, 938
BssMI GATC 4 cut(s) 325, 331, 398, 574
BssNAI GTATAC 1 cut(s) 769
BssT1I CCWWGG 2 cut(s) 608, 938
Bst1107I GTATAC 1 cut(s) 769
Bst2UI CCWGG 3 cut(s) 309, 522, 570
Bst4CI ACNGT 3 cut(s) 346, 492, 632
Bst6I CTCTTC 3 cut(s) 267, 344, 366
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 4 cut(s) 42, 378, 477, 720
BstDEI CTNAG 3 cut(s) 131, 336, 707
BstDSI CCRYGG 2 cut(s) 608, 938
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 4 cut(s) 328, 334, 401, 577
BstMBI GATC 4 cut(s) 325, 331, 398, 574
BstMWI GCNNNNNNNGC 2 cut(s) 56, 242
BstNI CCWGG 3 cut(s) 309, 522, 570
BstSCI CCNGG 5 cut(s) 94, 95, 307, 520, 568
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 3 cut(s) 104, 586, 975
BstV1I GCAGC 1 cut(s) 769
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 2 cut(s) 331, 398
BstYI RGATCY 2 cut(s) 331, 398
BstZ17I GTATAC 1 cut(s) 769
BsuRI GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
BtgI CCRYGG 2 cut(s) 608, 938
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 243, 351, 488
Cac8I GCNNGC 4 cut(s) 42, 378, 477, 720
CaiI CAGNNNCTG 1 cut(s) 968
Cfr13I GGNCC 3 cut(s) 100, 101, 861
Cfr9I CCCGGG 1 cut(s) 95
Csp6I GTAC 2 cut(s) 284, 460
CviAII CATG 8 cut(s) 45, 164, 209, 230, 609, 939, 953, 1009
CviQI GTAC 2 cut(s) 284, 460
DdeI CTNAG 3 cut(s) 131, 336, 707
DpnI GATC 4 cut(s) 327, 333, 400, 576
DpnII GATC 4 cut(s) 325, 331, 398, 574
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 2 cut(s) 183, 518
Eam1104I CTCTTC 3 cut(s) 267, 344, 366
EarI CTCTTC 3 cut(s) 267, 344, 366
EciI GGCGGA 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 608, 938
Eco24I GRGCYC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 307, 520, 568
EcoT14I CCWWGG 2 cut(s) 608, 938
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 608, 938
FaeI CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 879, 911
FaqI GGGAC 1 cut(s) 884
FatI CATG 8 cut(s) 44, 163, 208, 229, 608, 938, 952, 1008
FblI GTMKAC 2 cut(s) 542, 768
Fnu4HI GCNGC 1 cut(s) 758
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 1 cut(s) 758
FspBI CTAG 2 cut(s) 120, 476
GluI GCNGC 1 cut(s) 758
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 3 cut(s) 466, 501, 670
HaeIII GGCC 6 cut(s) 59, 102, 152, 185, 520, 745
HapII CCGG 1 cut(s) 96
Hin1II CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
HincII GTYRAC 3 cut(s) 508, 543, 960
HindII GTYRAC 3 cut(s) 508, 543, 960
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 4 cut(s) 12, 393, 773, 838
HpaI GTTAAC 1 cut(s) 960
HpaII CCGG 1 cut(s) 96
HphI GGTGA 4 cut(s) 249, 329, 590, 893
Hpy166II GTNNAC 6 cut(s) 286, 508, 543, 671, 769, 960
Hpy188I TCNGA 4 cut(s) 78, 392, 398, 636
Hpy188III TCNNGA 6 cut(s) 225, 329, 445, 692, 785, 835
Hpy8I GTNNAC 6 cut(s) 286, 508, 543, 671, 769, 960
HpyAV CCTTC 3 cut(s) 264, 657, 851
HpyCH4III ACNGT 3 cut(s) 346, 492, 632
HpyCH4V TGCA 3 cut(s) 236, 718, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 242
HpyF3I CTNAG 3 cut(s) 131, 336, 707
Hsp92II CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
KspAI GTTAAC 1 cut(s) 960
Kzo9I GATC 4 cut(s) 325, 331, 398, 574
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 5 cut(s) 121, 160, 447, 482, 502
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 3 cut(s) 223, 538, 705
MaeI CTAG 2 cut(s) 120, 476
MaeIII GTNAC 1 cut(s) 785
MalI GATC 4 cut(s) 327, 333, 400, 576
MboI GATC 4 cut(s) 325, 331, 398, 574
MboII GAAGA 9 cut(s) 91, 153, 213, 284, 361, 383, 446, 724, 899
MflI RGATCY 2 cut(s) 331, 398
MhlI GDGCHC 3 cut(s) 104, 586, 975
MlsI TGGCCA 2 cut(s) 185, 520
MluCI AATT 3 cut(s) 263, 404, 638
MluNI TGGCCA 2 cut(s) 185, 520
MlyI GAGTC 2 cut(s) 21, 782
MmeI TCCRAC 2 cut(s) 13, 397
MnlI CCTC 2 cut(s) 84, 477
Mox20I TGGCCA 2 cut(s) 185, 520
MscI TGGCCA 2 cut(s) 185, 520
MseI TTAA 2 cut(s) 959, 1013
MslI CAYNNNNRTG 1 cut(s) 622
Msp20I TGGCCA 2 cut(s) 185, 520
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 5 cut(s) 96, 97, 309, 522, 570
MvaI CCWGG 3 cut(s) 309, 522, 570
MwoI GCNNNNNNNGC 2 cut(s) 56, 242
NciI CCSGG 2 cut(s) 96, 97
NcoI CCATGG 2 cut(s) 608, 938
NdeII GATC 4 cut(s) 325, 331, 398, 574
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 8 cut(s) 48, 167, 212, 233, 612, 942, 956, 1012
NlaIV GGNNCC 5 cut(s) 91, 101, 102, 400, 862
NmuCI GTSAC 1 cut(s) 785
PasI CCCWGGG 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 393, 838
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 1 cut(s) 759
PleI GAGTC 2 cut(s) 20, 781
PpsI GAGTC 2 cut(s) 20, 781
Psp6I CCWGG 3 cut(s) 307, 520, 568
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 3 cut(s) 307, 520, 568
PspN4I GGNNCC 5 cut(s) 91, 101, 102, 400, 862
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 3 cut(s) 100, 101, 861
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 2 cut(s) 331, 398
RsaI GTAC 2 cut(s) 285, 461
RsaNI GTAC 2 cut(s) 284, 460
RseI CAYNNNNRTG 1 cut(s) 622
SalI GTCGAC 1 cut(s) 541
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 2 cut(s) 959, 1013
SatI GCNGC 1 cut(s) 758
Sau3AI GATC 4 cut(s) 325, 331, 398, 574
Sau96I GGNCC 3 cut(s) 100, 101, 861
SchI GAGTC 2 cut(s) 21, 782
ScrFI CCNGG 5 cut(s) 96, 97, 309, 522, 570
SduI GDGCHC 3 cut(s) 104, 586, 975
SfaNI GCATC 3 cut(s) 223, 538, 705
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 1 cut(s) 861
SmaI CCCGGG 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 622
Sse9I AATT 3 cut(s) 263, 404, 638
SsiI CCGC 3 cut(s) 68, 87, 432
SspMI CTAG 2 cut(s) 120, 476
StyD4I CCNGG 5 cut(s) 94, 95, 307, 520, 568
StyI CCWWGG 2 cut(s) 608, 938
TaaI ACNGT 3 cut(s) 346, 492, 632
TaqI TCGA 5 cut(s) 173, 270, 542, 589, 776
TasI AATT 3 cut(s) 263, 404, 638
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 2 cut(s) 393, 838
Tru1I TTAA 2 cut(s) 959, 1013
Tru9I TTAA 2 cut(s) 959, 1013
TscAI CASTG 3 cut(s) 243, 351, 495
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 1 cut(s) 757
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 5 cut(s) 197, 639, 870, 900, 1017
TspGWI ACGGA 2 cut(s) 765, 853
TspMI CCCGGG 1 cut(s) 95
TspRI CASTG 3 cut(s) 243, 351, 495
Van91I CCANNNNNTGG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 861
XapI RAATTY 2 cut(s) 263, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmaI CCCGGG 1 cut(s) 95
XmiI GTMKAC 2 cut(s) 542, 768
XspI CTAG 2 cut(s) 120, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.