AT1G74300
ERF Family

BEST Arabidopsis thaliana protein match is alpha beta-Hydrolases superfamily protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000873
Physical Location & Seq
Forward (+)
27935156 .. 27936608
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74300.1

Sequence Viewer

Length: 1041 bp
ATGGCGATTGGACCATCAAGTGTGATCATTATTGTTGGGATAATCTTGGCGTTGACTTATCAGTCAATCTTGAAACCTCCTCCACCGAATCTATGTGGCTCCCCCGGTGGTCCACCGATCACAGCACCGCGTATAAAGCTGCGAGACGGGAGACATTTGGCATACAAGGAGTATGGACTTCCAAGAGAGAAAGCTAAACACAAAATAGTCTTCATCCATGGATCCGATAGTTGTAGACACGACGCCGTTTTCGCCACTCTTCTTTCTCCGGATTTAGTTCAAGAACGAGGCGTGTATATGGTTTCGTTTGACAAACCGGGTTATGGAGAGAGTGATCCGGATCCAATCCGTACACCAAAAAGCTTGGCTTTGGATATTGAAGAGCTAGCTGATCAACTGAGTCTAGGATCAAAGTTCTACGTGATTGGGAAATCAATGGGAGGACAAGCGGCGTGGGGATGCCTTAAGTACACCCCTCACCGGTTAGCCGGAGTAACACTAGTTGCTCCTGTGGTTAACTACTATTGGAGGAACTTACCTTTGAACATTTCCACTGAAGGATTTAACTTGCAGCAAAAGAGAGATCAATGGGCGGTTCGTGTTGCTCACTACGCTCCTTGGCTTATTTACTGGTGGAACACACAAAACTGGTTCCCGGGTTCGAGTGTTGTGAACCGAGATGGCGGTGTCTTGTCACAGCCCGATAAAGATATCATTTTGAAGCTGGGATCTTCGAGGAAACCGCATTTGGCAGAAGTAAGGCAGCAAGGAATACATGAGAGTATTAACCGTGATATGATTGTGGGTTTTGGAAATTGGGAATTTGATCCTTTAGAACTCGAGAATCCGTTTTTAAACAGAGAAGGCTCTGTTCATTTGTGGCAAGGAGACGAGGACATGTTAGTGCCTGTGACGCTGCAACGTTACATTGCAGATAAGCTTCCTTGGCTTCACTATCATGAGGTTGCCGGAGGTGGCCACTTCTTCCCCCTCGCTAAAGGTGTCGTTGATGAGATTGTTAAGACAGCTCTTAACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

346

Amino Acids

38.71

Weight (kDa)

7.23

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 64 - 178 3.3e-08 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 69 - 331 8.5e-11 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 88 - 329 4.4e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 235
AccII CGCG 1 cut(s) 130
AccIII TCCGGA 2 cut(s) 268, 337
AciI CCGC 5 cut(s) 128, 449, 593, 684, 743
AclI AACGTT 1 cut(s) 922
AclWI GGATC 8 cut(s) 216, 229, 329, 335, 348, 415, 736, 821
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 352, 470
AfiI CCNNNNNNNGG 2 cut(s) 323, 480
AflII CTTAAG 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 897
AgeI ACCGGT 1 cut(s) 480
AgsI TTSAA 5 cut(s) 73, 281, 380, 544, 721
AhlI ACTAGT 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AluBI AGCT 8 cut(s) 139, 194, 363, 385, 389, 724, 940, 1028
AluI AGCT 8 cut(s) 139, 194, 363, 385, 389, 724, 940, 1028
Alw26I GTCTC 3 cut(s) 138, 145, 882
AlwI GGATC 8 cut(s) 216, 229, 329, 335, 348, 415, 736, 821
Ama87I CYCGRG 2 cut(s) 655, 839
Aor13HI TCCGGA 2 cut(s) 268, 337
AoxI GGCC 1 cut(s) 976
ApeKI GCWGC 4 cut(s) 139, 571, 763, 916
ApoI RAATTY 1 cut(s) 821
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
AsiGI ACCGGT 1 cut(s) 480
AspS9I GGNCC 2 cut(s) 11, 110
AsuC2I CCSGG 4 cut(s) 105, 318, 656, 657
AsuHPI GGTGA 1 cut(s) 470
AsuNHI GCTAGC 1 cut(s) 385
AvaI CYCGRG 2 cut(s) 655, 839
AvaII GGWCC 2 cut(s) 11, 110
BalI TGGCCA 1 cut(s) 978
BamHI GGATCC 2 cut(s) 221, 340
BbsI GAAGAC 1 cut(s) 202
BbvI GCAGC 4 cut(s) 126, 583, 775, 903
BccI CCATC 2 cut(s) 22, 674
BceAI ACGGC 1 cut(s) 230
BclI TGATCA 2 cut(s) 24, 391
BcnI CCSGG 4 cut(s) 105, 318, 656, 657
BcoDI GTCTC 3 cut(s) 138, 145, 882
BcuI ACTAGT 1 cut(s) 499
BfaI CTAG 3 cut(s) 386, 404, 500
BfrI CTTAAG 1 cut(s) 464
BisI GCNGC 5 cut(s) 140, 450, 572, 764, 917
BlsI GCNGC 5 cut(s) 141, 451, 573, 765, 918
Bme1390I CCNGG 4 cut(s) 105, 318, 656, 657
Bme18I GGWCC 2 cut(s) 11, 110
BmeT110I CYCGRG 2 cut(s) 655, 839
BmgT120I GGNCC 2 cut(s) 11, 110
BmiI GGNNCC 4 cut(s) 100, 223, 342, 653
BmrFI CCNGG 4 cut(s) 105, 318, 656, 657
BmsI GCATC 1 cut(s) 449
BmtI GCTAGC 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 202
BpuMI CCSGG 4 cut(s) 105, 318, 656, 657
BsaAI YACGTR 1 cut(s) 421
BsaBI GATNNNNATC 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 5 cut(s) 103, 217, 617, 655, 944
BsaWI WCCGGW 3 cut(s) 268, 337, 480
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 480
Bse118I RCCGGY 1 cut(s) 480
Bse1I ACTGG 2 cut(s) 635, 653
Bse3DI GCAATG 1 cut(s) 927
Bse8I GATNNNNATC 1 cut(s) 339
BseAI TCCGGA 2 cut(s) 268, 337
BseDI CCNNGG 5 cut(s) 103, 217, 617, 655, 944
BseGI GGATG 2 cut(s) 213, 464
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 2 cut(s) 323, 480
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 1 cut(s) 389
BseNI ACTGG 2 cut(s) 635, 653
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 4 cut(s) 126, 583, 775, 903
BseYI CCCAGC 1 cut(s) 724
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 1 cut(s) 978
BshTI ACCGGT 1 cut(s) 480
BsiHKCI CYCGRG 2 cut(s) 655, 839
BsiSI CCGG 8 cut(s) 105, 269, 317, 338, 481, 489, 656, 969
BslI CCNNNNNNNGG 2 cut(s) 323, 480
BsmAI GTCTC 3 cut(s) 138, 145, 882
BsmBI CGTCTC 2 cut(s) 138, 882
BsnI GGCC 1 cut(s) 978
BsoBI CYCGRG 2 cut(s) 655, 839
Bsp13I TCCGGA 2 cut(s) 268, 337
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 5 cut(s) 128, 449, 593, 684, 743
BspANI GGCC 1 cut(s) 978
BspCNI CTCAG 1 cut(s) 390
BspEI TCCGGA 2 cut(s) 268, 337
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 4 cut(s) 100, 223, 342, 653
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 8 cut(s) 216, 229, 329, 335, 348, 415, 736, 821
BspQI GCTCTTC 1 cut(s) 375
BspTI CTTAAG 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 1 cut(s) 480
BsrI ACTGG 2 cut(s) 635, 653
BssAI RCCGGY 1 cut(s) 480
BssECI CCNNGG 5 cut(s) 103, 217, 617, 655, 944
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 3 cut(s) 217, 617, 944
Bst4CI ACNGT 1 cut(s) 791
Bst6I CTCTTC 2 cut(s) 264, 375
BstACI GRCGYC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 398
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 2 cut(s) 213, 464
BstFNI CGCG 1 cut(s) 130
BstMAI GTCTC 3 cut(s) 138, 145, 882
BstMWI GCNNNNNNNGC 5 cut(s) 136, 251, 611, 913, 946
BstNSI RCATGY 1 cut(s) 901
BstSCI CCNGG 4 cut(s) 103, 316, 654, 655
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 4 cut(s) 126, 583, 775, 903
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 3 cut(s) 221, 340, 728
BstYI RGATCY 3 cut(s) 221, 340, 728
BsuRI GGCC 1 cut(s) 978
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 2 cut(s) 213, 464
BtsIMutI CAGTG 2 cut(s) 552, 1036
Cac8I GCNNGC 1 cut(s) 387
CciI TCATGA 1 cut(s) 958
Cfr10I RCCGGY 1 cut(s) 480
Cfr13I GGNCC 2 cut(s) 11, 110
Cfr9I CCCGGG 1 cut(s) 655
CseI GACGC 2 cut(s) 251, 922
Csp6I GTAC 2 cut(s) 351, 469
CspAI ACCGGT 1 cut(s) 480
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviAII CATG 4 cut(s) 218, 776, 898, 959
CviQI GTAC 2 cut(s) 351, 469
DdeI CTNAG 1 cut(s) 398
DraI TTTAAA 1 cut(s) 855
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 2 cut(s) 264, 375
EarI CTCTTC 2 cut(s) 264, 375
Eco130I CCWWGG 3 cut(s) 217, 617, 944
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 2 cut(s) 11, 110
Eco57I CTGAAG 1 cut(s) 576
Eco88I CYCGRG 2 cut(s) 655, 839
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 3 cut(s) 217, 617, 944
ErhI CCWWGG 3 cut(s) 217, 617, 944
Esp3I CGTCTC 2 cut(s) 138, 882
FaeI CATG 4 cut(s) 221, 779, 901, 962
FalI AAGNNNNNCTT 2 cut(s) 352, 384
FatI CATG 4 cut(s) 217, 775, 897, 958
FbaI TGATCA 2 cut(s) 24, 391
FblI GTMKAC 1 cut(s) 235
Fnu4HI GCNGC 5 cut(s) 140, 450, 572, 764, 917
FokI GGATG 2 cut(s) 200, 471
Fsp4HI GCNGC 5 cut(s) 140, 450, 572, 764, 917
FspBI CTAG 3 cut(s) 386, 404, 500
GluI GCNGC 5 cut(s) 140, 450, 572, 764, 917
GsaI CCCAGC 1 cut(s) 728
HaeIII GGCC 1 cut(s) 978
HapII CCGG 8 cut(s) 105, 269, 317, 338, 481, 489, 656, 969
HgaI GACGC 2 cut(s) 251, 922
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 4 cut(s) 221, 779, 901, 962
HincII GTYRAC 2 cut(s) 54, 517
HindII GTYRAC 2 cut(s) 54, 517
HindIII AAGCTT 2 cut(s) 361, 938
HinfI GANTC 3 cut(s) 88, 400, 844
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 8 cut(s) 105, 269, 317, 338, 481, 489, 656, 969
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 7 cut(s) 54, 113, 236, 353, 471, 517, 673
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 6 cut(s) 70, 269, 281, 338, 841, 959
Hpy8I GTNNAC 7 cut(s) 54, 113, 236, 353, 471, 517, 673
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 551, 857
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4IV ACGT 2 cut(s) 420, 922
HpyCH4V TGCA 3 cut(s) 571, 919, 932
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 251, 611, 913, 946
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 2 cut(s) 420, 922
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 4 cut(s) 221, 779, 901, 962
Kpn2I TCCGGA 2 cut(s) 268, 337
Ksp22I TGATCA 2 cut(s) 24, 391
KspAI GTTAAC 1 cut(s) 517
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 3 cut(s) 104, 511, 619
Lsp1109I GCAGC 4 cut(s) 126, 583, 775, 903
LweI GCATC 1 cut(s) 449
MaeI CTAG 3 cut(s) 386, 404, 500
MaeII ACGT 2 cut(s) 420, 922
MaeIII GTNAC 4 cut(s) 493, 693, 910, 923
MboII GAAGA 5 cut(s) 202, 251, 392, 723, 976
MflI RGATCY 3 cut(s) 221, 340, 728
MlsI TGGCCA 1 cut(s) 978
MluCI AATT 2 cut(s) 814, 821
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 1 cut(s) 409
Mox20I TGGCCA 1 cut(s) 978
MroI TCCGGA 2 cut(s) 268, 337
MscI TGGCCA 1 cut(s) 978
MseI TTAA 7 cut(s) 465, 516, 564, 786, 854, 1020, 1032
MslI CAYNNNNRTG 2 cut(s) 902, 957
Msp20I TGGCCA 1 cut(s) 978
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 8 cut(s) 105, 269, 317, 338, 481, 489, 656, 969
MspR9I CCNGG 4 cut(s) 105, 318, 656, 657
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 5 cut(s) 136, 251, 611, 913, 946
NciI CCSGG 4 cut(s) 105, 318, 656, 657
NcoI CCATGG 1 cut(s) 217
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 4 cut(s) 221, 779, 901, 962
NlaIV GGNNCC 4 cut(s) 100, 223, 342, 653
NmuCI GTSAC 2 cut(s) 693, 910
NspI RCATGY 1 cut(s) 901
PaeR7I CTCGAG 1 cut(s) 839
PagI TCATGA 1 cut(s) 958
PciI ACATGT 1 cut(s) 897
PciSI GCTCTTC 1 cut(s) 375
PfeI GAWTC 2 cut(s) 88, 844
PinAI ACCGGT 1 cut(s) 480
PkrI GCNGC 5 cut(s) 141, 451, 573, 765, 918
PleI GAGTC 1 cut(s) 408
PpsI GAGTC 1 cut(s) 408
Ppu21I YACGTR 1 cut(s) 421
PscI ACATGT 1 cut(s) 897
Psp1406I AACGTT 1 cut(s) 922
PspFI CCCAGC 1 cut(s) 724
PspN4I GGNNCC 4 cut(s) 100, 223, 342, 653
PspPI GGNCC 2 cut(s) 11, 110
PsuI RGATCY 3 cut(s) 221, 340, 728
RsaI GTAC 2 cut(s) 352, 470
RsaNI GTAC 2 cut(s) 351, 469
RseI CAYNNNNRTG 2 cut(s) 902, 957
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 7 cut(s) 465, 516, 564, 786, 854, 1020, 1032
SatI GCNGC 5 cut(s) 140, 450, 572, 764, 917
Sau96I GGNCC 2 cut(s) 11, 110
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 4 cut(s) 105, 318, 656, 657
SfaNI GCATC 1 cut(s) 449
Sfr274I CTCGAG 1 cut(s) 839
SinI GGWCC 2 cut(s) 11, 110
SlaI CTCGAG 1 cut(s) 839
SmaI CCCGGG 1 cut(s) 657
SmiMI CAYNNNNRTG 2 cut(s) 902, 957
SmlI CTYRAG 2 cut(s) 464, 839
SmoI CTYRAG 2 cut(s) 464, 839
SpeI ACTAGT 1 cut(s) 499
Sse9I AATT 2 cut(s) 814, 821
SsiI CCGC 5 cut(s) 128, 449, 593, 684, 743
SspMI CTAG 3 cut(s) 386, 404, 500
StyD4I CCNGG 4 cut(s) 103, 316, 654, 655
StyI CCWWGG 3 cut(s) 217, 617, 944
TaaI ACNGT 1 cut(s) 791
TaiI ACGT 2 cut(s) 423, 925
TaqI TCGA 3 cut(s) 662, 734, 840
TasI AATT 2 cut(s) 814, 821
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 2 cut(s) 88, 844
Tru1I TTAA 7 cut(s) 465, 516, 564, 786, 854, 1020, 1032
Tru9I TTAA 7 cut(s) 465, 516, 564, 786, 854, 1020, 1032
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 2 cut(s) 693, 910
TseI GCWGC 4 cut(s) 139, 571, 763, 916
Tsp45I GTSAC 2 cut(s) 693, 910
TspDTI ATGAA 2 cut(s) 202, 863
TspGWI ACGGA 2 cut(s) 338, 837
TspMI CCCGGG 1 cut(s) 655
TspRI CASTG 1 cut(s) 559
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 11, 110
XapI RAATTY 1 cut(s) 821
XceI RCATGY 1 cut(s) 901
XhoI CTCGAG 1 cut(s) 839
XmaI CCCGGG 1 cut(s) 655
XmiI GTMKAC 1 cut(s) 235
XspI CTAG 3 cut(s) 386, 404, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.