Alpha/beta hydrolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 961 |
| AccB1I | GGYRCC | 1 cut(s) | 961 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 971 |
| AccII | CGCG | 1 cut(s) | 789 |
| AccIII | TCCGGA | 2 cut(s) | 259, 328 |
| AciI | CCGC | 4 cut(s) | 60, 99, 569, 691 |
| AclWI | GGATC | 7 cut(s) | 20, 320, 339, 393, 406, 582, 840 |
| AcoI | YGGCCR | 1 cut(s) | 518 |
| AcsI | RAATTY | 2 cut(s) | 638, 818 |
| AcuI | CTGAAG | 2 cut(s) | 199, 440 |
| AfaI | GTAC | 5 cut(s) | 191, 287, 314, 461, 963 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 314, 624, 700, 971 |
| AflII | CTTAAG | 1 cut(s) | 335 |
| AflIII | ACRYGT | 1 cut(s) | 751 |
| AgsI | TTSAA | 1 cut(s) | 272 |
| AjnI | CCWGG | 4 cut(s) | 94, 307, 520, 964 |
| Alw26I | GTCTC | 1 cut(s) | 259 |
| AlwI | GGATC | 7 cut(s) | 20, 320, 339, 393, 406, 582, 840 |
| Aor13HI | TCCGGA | 2 cut(s) | 259, 328 |
| AoxI | GGCC | 3 cut(s) | 57, 150, 518 |
| ApeKI | GCWGC | 5 cut(s) | 485, 593, 760, 913, 989 |
| ApoI | RAATTY | 2 cut(s) | 638, 818 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 852, 884 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 936 |
| Asp718I | GGTACC | 1 cut(s) | 961 |
| AspLEI | GCGC | 2 cut(s) | 118, 586 |
| AspS9I | GGNCC | 2 cut(s) | 101, 868 |
| AsuC2I | CCSGG | 1 cut(s) | 667 |
| AsuHPI | GGTGA | 3 cut(s) | 392, 514, 896 |
| AsuNHI | GCTAGC | 1 cut(s) | 475 |
| AvaII | GGWCC | 2 cut(s) | 101, 868 |
| BalI | TGGCCA | 1 cut(s) | 520 |
| BamHI | GGATCC | 1 cut(s) | 398 |
| BanI | GGYRCC | 1 cut(s) | 961 |
| BbsI | GAAGAC | 1 cut(s) | 446 |
| BbvI | GCAGC | 5 cut(s) | 497, 580, 772, 900, 976 |
| BccI | CCATC | 5 cut(s) | 89, 131, 788, 879, 989 |
| BciT130I | CCWGG | 4 cut(s) | 96, 309, 522, 966 |
| BciVI | GTATCC | 2 cut(s) | 413, 673 |
| BclI | TGATCA | 2 cut(s) | 382, 504 |
| BcnI | CCSGG | 1 cut(s) | 667 |
| BcoDI | GTCTC | 1 cut(s) | 259 |
| BfaI | CTAG | 2 cut(s) | 120, 476 |
| BfmI | CTRYAG | 2 cut(s) | 836, 990 |
| BfoI | RGCGCY | 2 cut(s) | 119, 587 |
| BfrI | CTTAAG | 1 cut(s) | 335 |
| BfuI | GTATCC | 2 cut(s) | 413, 673 |
| BisI | GCNGC | 6 cut(s) | 60, 486, 594, 761, 914, 990 |
| BlsI | GCNGC | 6 cut(s) | 61, 487, 595, 762, 915, 991 |
| Bme1390I | CCNGG | 5 cut(s) | 96, 309, 522, 667, 966 |
| Bme18I | GGWCC | 2 cut(s) | 101, 868 |
| BmgT120I | GGNCC | 2 cut(s) | 101, 868 |
| BmiI | GGNNCC | 2 cut(s) | 400, 963 |
| BmrFI | CCNGG | 5 cut(s) | 96, 309, 522, 667, 966 |
| BmsI | GCATC | 1 cut(s) | 450 |
| BmtI | GCTAGC | 1 cut(s) | 479 |
| BpiI | GAAGAC | 1 cut(s) | 446 |
| Bpu10I | CCTNAGC | 1 cut(s) | 131 |
| BpuMI | CCSGG | 1 cut(s) | 667 |
| BsaBI | GATNNNNATC | 1 cut(s) | 330 |
| BsaJI | CCNNGG | 3 cut(s) | 308, 608, 941 |
| BsaWI | WCCGGW | 3 cut(s) | 259, 328, 499 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 314, 624, 700, 971 |
| Bse1I | ACTGG | 3 cut(s) | 518, 626, 905 |
| Bse8I | GATNNNNATC | 1 cut(s) | 330 |
| BseAI | TCCGGA | 2 cut(s) | 259, 328 |
| BseBI | CCWGG | 4 cut(s) | 96, 309, 522, 966 |
| BseDI | CCNNGG | 3 cut(s) | 308, 608, 941 |
| BseGI | GGATG | 3 cut(s) | 142, 208, 462 |
| BseJI | GATNNNNATC | 1 cut(s) | 330 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 314, 624, 700, 971 |
| BseMII | CTCAG | 2 cut(s) | 647, 765 |
| BseNI | ACTGG | 3 cut(s) | 518, 626, 905 |
| BseRI | GAGGAG | 1 cut(s) | 386 |
| BseXI | GCAGC | 5 cut(s) | 497, 580, 772, 900, 976 |
| BseYI | CCCAGC | 1 cut(s) | 813 |
| Bsh1236I | CGCG | 1 cut(s) | 789 |
| BshFI | GGCC | 3 cut(s) | 59, 152, 520 |
| BshNI | GGYRCC | 1 cut(s) | 961 |
| BsiSI | CCGG | 4 cut(s) | 260, 329, 500, 666 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 314, 624, 700, 971 |
| BsmAI | GTCTC | 1 cut(s) | 259 |
| BsmBI | CGTCTC | 1 cut(s) | 259 |
| BsmI | GAATGC | 1 cut(s) | 721 |
| BsnI | GGCC | 3 cut(s) | 59, 152, 520 |
| Bsp13I | TCCGGA | 2 cut(s) | 259, 328 |
| Bsp1407I | TGTACA | 1 cut(s) | 285 |
| Bsp19I | CCATGG | 1 cut(s) | 941 |
| Bsp68I | TCGCGA | 1 cut(s) | 789 |
| BspACI | CCGC | 4 cut(s) | 60, 99, 569, 691 |
| BspANI | GGCC | 3 cut(s) | 59, 152, 520 |
| BspCNI | CTCAG | 2 cut(s) | 646, 766 |
| BspEI | TCCGGA | 2 cut(s) | 259, 328 |
| BspFNI | CGCG | 1 cut(s) | 789 |
| BspLI | GGNNCC | 2 cut(s) | 400, 963 |
| BspMAI | CTGCAG | 2 cut(s) | 840, 994 |
| BspOI | GCTAGC | 1 cut(s) | 479 |
| BspPI | GGATC | 7 cut(s) | 20, 320, 339, 393, 406, 582, 840 |
| BspT107I | GGYRCC | 1 cut(s) | 961 |
| BspTI | CTTAAG | 1 cut(s) | 335 |
| BsrGI | TGTACA | 1 cut(s) | 285 |
| BsrI | ACTGG | 3 cut(s) | 518, 626, 905 |
| BssECI | CCNNGG | 3 cut(s) | 308, 608, 941 |
| BssT1I | CCWWGG | 2 cut(s) | 608, 941 |
| Bst2UI | CCWGG | 4 cut(s) | 96, 309, 522, 966 |
| Bst4CI | ACNGT | 1 cut(s) | 438 |
| Bst6I | CTCTTC | 1 cut(s) | 727 |
| BstAFI | CTTAAG | 1 cut(s) | 335 |
| BstAUI | TGTACA | 1 cut(s) | 285 |
| BstC8I | GCNNGC | 3 cut(s) | 42, 477, 853 |
| BstDEI | CTNAG | 3 cut(s) | 131, 633, 774 |
| BstDSI | CCRYGG | 1 cut(s) | 941 |
| BstF5I | GGATG | 3 cut(s) | 142, 208, 462 |
| BstFNI | CGCG | 1 cut(s) | 789 |
| BstH2I | RGCGCY | 2 cut(s) | 119, 587 |
| BstHHI | GCGC | 2 cut(s) | 118, 586 |
| BstMAI | GTCTC | 1 cut(s) | 259 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 56, 485, 494, 568, 590, 861, 910 |
| BstNI | CCWGG | 4 cut(s) | 96, 309, 522, 966 |
| BstNSI | RCATGY | 1 cut(s) | 755 |
| BstSCI | CCNGG | 5 cut(s) | 94, 307, 520, 665, 964 |
| BstSFI | CTRYAG | 2 cut(s) | 836, 990 |
| BstUI | CGCG | 1 cut(s) | 789 |
| BstV1I | GCAGC | 5 cut(s) | 497, 580, 772, 900, 976 |
| BstV2I | GAAGAC | 1 cut(s) | 446 |
| BstX2I | RGATCY | 3 cut(s) | 331, 398, 832 |
| BstYI | RGATCY | 3 cut(s) | 331, 398, 832 |
| BsuI | GTATCC | 2 cut(s) | 413, 673 |
| BsuRI | GGCC | 3 cut(s) | 59, 152, 520 |
| BtgI | CCRYGG | 1 cut(s) | 941 |
| BtsCI | GGATG | 3 cut(s) | 142, 208, 462 |
| BtsIMutI | CAGTG | 2 cut(s) | 443, 488 |
| BtuMI | TCGCGA | 1 cut(s) | 789 |
| Cac8I | GCNNGC | 3 cut(s) | 42, 477, 853 |
| CfoI | GCGC | 2 cut(s) | 118, 586 |
| Cfr13I | GGNCC | 2 cut(s) | 101, 868 |
| CsiI | ACCWGGT | 1 cut(s) | 964 |
| Csp6I | GTAC | 5 cut(s) | 190, 286, 313, 460, 962 |
| CviAII | CATG | 5 cut(s) | 164, 752, 793, 942, 956 |
| CviQI | GTAC | 5 cut(s) | 190, 286, 313, 460, 962 |
| DdeI | CTNAG | 3 cut(s) | 131, 633, 774 |
| EaeI | YGGCCR | 1 cut(s) | 518 |
| Eam1104I | CTCTTC | 1 cut(s) | 727 |
| EarI | CTCTTC | 1 cut(s) | 727 |
| Eco130I | CCWWGG | 2 cut(s) | 608, 941 |
| Eco47I | GGWCC | 2 cut(s) | 101, 868 |
| Eco57I | CTGAAG | 2 cut(s) | 199, 440 |
| EcoRII | CCWGG | 4 cut(s) | 94, 307, 520, 964 |
| EcoT14I | CCWWGG | 2 cut(s) | 608, 941 |
| ErhI | CCWWGG | 2 cut(s) | 608, 941 |
| Esp3I | CGTCTC | 1 cut(s) | 259 |
| FaeI | CATG | 5 cut(s) | 167, 755, 796, 945, 959 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 185, 217, 882, 914 |
| FatI | CATG | 5 cut(s) | 163, 751, 792, 941, 955 |
| FauI | CCCGC | 1 cut(s) | 698 |
| FbaI | TGATCA | 2 cut(s) | 382, 504 |
| Fnu4HI | GCNGC | 6 cut(s) | 60, 486, 594, 761, 914, 990 |
| FokI | GGATG | 3 cut(s) | 149, 195, 449 |
| Fsp4HI | GCNGC | 6 cut(s) | 60, 486, 594, 761, 914, 990 |
| FspBI | CTAG | 2 cut(s) | 120, 476 |
| GlaI | GCGC | 2 cut(s) | 117, 585 |
| GluI | GCNGC | 6 cut(s) | 60, 486, 594, 761, 914, 990 |
| GsaI | CCCAGC | 1 cut(s) | 817 |
| HaeII | RGCGCY | 2 cut(s) | 119, 587 |
| HaeIII | GGCC | 3 cut(s) | 59, 152, 520 |
| HapII | CCGG | 4 cut(s) | 260, 329, 500, 666 |
| HhaI | GCGC | 2 cut(s) | 118, 586 |
| Hin1II | CATG | 5 cut(s) | 167, 755, 796, 945, 959 |
| Hin6I | GCGC | 2 cut(s) | 116, 584 |
| HinP1I | GCGC | 2 cut(s) | 116, 584 |
| HincII | GTYRAC | 1 cut(s) | 615 |
| HindII | GTYRAC | 1 cut(s) | 615 |
| HindIII | AAGCTT | 2 cut(s) | 896, 935 |
| HinfI | GANTC | 2 cut(s) | 776, 946 |
| HpaI | GTTAAC | 1 cut(s) | 615 |
| HpaII | CCGG | 4 cut(s) | 260, 329, 500, 666 |
| HphI | GGTGA | 3 cut(s) | 392, 514, 896 |
| Hpy166II | GTNNAC | 4 cut(s) | 104, 286, 615, 974 |
| Hpy188I | TCNGA | 3 cut(s) | 78, 398, 636 |
| Hpy188III | TCNNGA | 6 cut(s) | 225, 260, 329, 445, 729, 788 |
| Hpy8I | GTNNAC | 4 cut(s) | 104, 286, 615, 974 |
| HpyAV | CCTTC | 2 cut(s) | 223, 854 |
| HpyCH4III | ACNGT | 1 cut(s) | 438 |
| HpyCH4V | TGCA | 6 cut(s) | 441, 719, 838, 916, 992, 1022 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 56, 485, 494, 568, 590, 861, 910 |
| HpyF3I | CTNAG | 3 cut(s) | 131, 633, 774 |
| Hsp92II | CATG | 5 cut(s) | 167, 755, 796, 945, 959 |
| HspAI | GCGC | 2 cut(s) | 116, 584 |
| Kpn2I | TCCGGA | 2 cut(s) | 259, 328 |
| KpnI | GGTACC | 1 cut(s) | 965 |
| Ksp22I | TGATCA | 2 cut(s) | 382, 504 |
| KspAI | GTTAAC | 1 cut(s) | 615 |
| LmnI | GCTCC | 4 cut(s) | 373, 482, 502, 810 |
| Lsp1109I | GCAGC | 5 cut(s) | 497, 580, 772, 900, 976 |
| LweI | GCATC | 1 cut(s) | 450 |
| MabI | ACCWGGT | 1 cut(s) | 964 |
| MaeI | CTAG | 2 cut(s) | 120, 476 |
| MaeIII | GTNAC | 2 cut(s) | 754, 884 |
| MboII | GAAGA | 5 cut(s) | 153, 284, 446, 744, 902 |
| MflI | RGATCY | 3 cut(s) | 331, 398, 832 |
| MlsI | TGGCCA | 1 cut(s) | 520 |
| MluCI | AATT | 5 cut(s) | 403, 414, 638, 818, 1029 |
| MluNI | TGGCCA | 1 cut(s) | 520 |
| MlyI | GAGTC | 1 cut(s) | 785 |
| MmeI | TCCRAC | 3 cut(s) | 13, 101, 781 |
| MnlI | CCTC | 7 cut(s) | 3, 84, 364, 425, 477, 576, 952 |
| Mox20I | TGGCCA | 1 cut(s) | 520 |
| MroI | TCCGGA | 2 cut(s) | 259, 328 |
| MroXI | GAANNNNTTC | 1 cut(s) | 936 |
| MscI | TGGCCA | 1 cut(s) | 520 |
| MseI | TTAA | 4 cut(s) | 6, 336, 614, 1016 |
| MslI | CAYNNNNRTG | 1 cut(s) | 954 |
| Msp20I | TGGCCA | 1 cut(s) | 520 |
| MspA1I | CMGCKG | 2 cut(s) | 388, 989 |
| MspCI | CTTAAG | 1 cut(s) | 335 |
| MspI | CCGG | 4 cut(s) | 260, 329, 500, 666 |
| MspR9I | CCNGG | 5 cut(s) | 96, 309, 522, 667, 966 |
| Mva1269I | GAATGC | 1 cut(s) | 721 |
| MvaI | CCWGG | 4 cut(s) | 96, 309, 522, 966 |
| MvnI | CGCG | 1 cut(s) | 789 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 56, 485, 494, 568, 590, 861, 910 |
| NciI | CCSGG | 1 cut(s) | 667 |
| NcoI | CCATGG | 1 cut(s) | 941 |
| NheI | GCTAGC | 1 cut(s) | 475 |
| NlaIII | CATG | 5 cut(s) | 167, 755, 796, 945, 959 |
| NlaIV | GGNNCC | 2 cut(s) | 400, 963 |
| NmuCI | GTSAC | 2 cut(s) | 754, 884 |
| NruI | TCGCGA | 1 cut(s) | 789 |
| NspI | RCATGY | 1 cut(s) | 755 |
| PciI | ACATGT | 1 cut(s) | 751 |
| PctI | GAATGC | 1 cut(s) | 721 |
| PdmI | GAANNNNTTC | 1 cut(s) | 936 |
| PfeI | GAWTC | 1 cut(s) | 946 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 971 |
| PfoI | TCCNGGA | 1 cut(s) | 665 |
| PkrI | GCNGC | 6 cut(s) | 61, 487, 595, 762, 915, 991 |
| PleI | GAGTC | 1 cut(s) | 784 |
| PpsI | GAGTC | 1 cut(s) | 784 |
| PscI | ACATGT | 1 cut(s) | 751 |
| Psp6I | CCWGG | 4 cut(s) | 94, 307, 520, 964 |
| PspFI | CCCAGC | 1 cut(s) | 813 |
| PspGI | CCWGG | 4 cut(s) | 94, 307, 520, 964 |
| PspN4I | GGNNCC | 2 cut(s) | 400, 963 |
| PspPI | GGNCC | 2 cut(s) | 101, 868 |
| PstI | CTGCAG | 2 cut(s) | 840, 994 |
| PsuI | RGATCY | 3 cut(s) | 331, 398, 832 |
| PvuII | CAGCTG | 2 cut(s) | 388, 989 |
| RruI | TCGCGA | 1 cut(s) | 789 |
| RsaI | GTAC | 5 cut(s) | 191, 287, 314, 461, 963 |
| RsaNI | GTAC | 5 cut(s) | 190, 286, 313, 460, 962 |
| RseI | CAYNNNNRTG | 1 cut(s) | 954 |
| SaqAI | TTAA | 4 cut(s) | 6, 336, 614, 1016 |
| SatI | GCNGC | 6 cut(s) | 60, 486, 594, 761, 914, 990 |
| Sau96I | GGNCC | 2 cut(s) | 101, 868 |
| SchI | GAGTC | 1 cut(s) | 785 |
| ScrFI | CCNGG | 5 cut(s) | 96, 309, 522, 667, 966 |
| SexAI | ACCWGGT | 1 cut(s) | 964 |
| SfaNI | GCATC | 1 cut(s) | 450 |
| SfcI | CTRYAG | 2 cut(s) | 836, 990 |
| SinI | GGWCC | 2 cut(s) | 101, 868 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 954 |
| SmlI | CTYRAG | 1 cut(s) | 335 |
| SmoI | CTYRAG | 1 cut(s) | 335 |
| Sse9I | AATT | 5 cut(s) | 403, 414, 638, 818, 1029 |
| SsiI | CCGC | 4 cut(s) | 60, 99, 569, 691 |
| SspMI | CTAG | 2 cut(s) | 120, 476 |
| StyD4I | CCNGG | 5 cut(s) | 94, 307, 520, 665, 964 |
| StyI | CCWWGG | 2 cut(s) | 608, 941 |
| TaaI | ACNGT | 1 cut(s) | 438 |
| TaqI | TCGA | 2 cut(s) | 300, 589 |
| TaqII | GACCGA | 1 cut(s) | 856 |
| TasI | AATT | 5 cut(s) | 403, 414, 638, 818, 1029 |
| TatI | WGTACW | 3 cut(s) | 189, 285, 459 |
| TauI | GCSGC | 1 cut(s) | 62 |
| TfiI | GAWTC | 1 cut(s) | 946 |
| Tru1I | TTAA | 4 cut(s) | 6, 336, 614, 1016 |
| Tru9I | TTAA | 4 cut(s) | 6, 336, 614, 1016 |
| TscAI | CASTG | 2 cut(s) | 443, 495 |
| TseFI | GTSAC | 2 cut(s) | 754, 884 |
| TseI | GCWGC | 5 cut(s) | 485, 593, 760, 913, 989 |
| Tsp45I | GTSAC | 2 cut(s) | 754, 884 |
| TspDTI | ATGAA | 2 cut(s) | 10, 197 |
| TspGWI | ACGGA | 1 cut(s) | 241 |
| TspRI | CASTG | 2 cut(s) | 443, 495 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 971 |
| Vha464I | CTTAAG | 1 cut(s) | 335 |
| VpaK11BI | GGWCC | 2 cut(s) | 101, 868 |
| XapI | RAATTY | 2 cut(s) | 638, 818 |
| XceI | RCATGY | 1 cut(s) | 755 |
| XmnI | GAANNNNTTC | 1 cut(s) | 936 |
| XspI | CTAG | 2 cut(s) | 120, 476 |