Rorug03G0130800

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000873
Physical Location & Seq
Reverse (-)
10618101 .. 10620069
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0130800.1

Sequence Viewer

Length: 1737 bp
ATGGAAGAAGGTTGGGGGAGAGGAGAGAACGCTATGGAGCATGCGATAATGCATTCTTCATCCCCTCCCAAACAACCAGTCGGAGGGTGGAAAGCCGTCAGATACATCCTTGGGAATGAGACCTTTGAGAAATTGGCTTCCATGAGTTTGGTGGCAAATCTGGTGCTGTATCTGCATACCAAGTATAACTTGGATAATGTAGTTTCAGCTAATGTATATAATATCTGGTCTGGTTCATGCAATGTTGCACCCTTAATTGGTGCTTACCTTGCTGATACTTTTTTGGGAAAGTACTCTACTCTTCTTTATAGCTCAATAGCATCCCTCATGGGTATGGTGGCCTTGACATTAACTGCAAGTTTACAGGAACTAACTCCTCCTGCCTGCACTGGACAAGCGCAGTGCGAACAACCCAATGCATGGCAGAAGTTTGTCCTCTTTTTGGGTCTGGGACTGCTCGTTATTGGATCTGGAGGCCTCAGACCATGCAACATTGCCTTTGGAGCTGATCAATTCGACACCACAACAGAGAAGGGAAGAGCACAGCTAGATAGCTTTTGCAATTGGTGGTACTTTTTGTTCACCATTGCTCTCATTGTAGCTCTGACCGGAGTGGTCTACATACAAACTCATATCAGCTGGGTTTTGGGTTTTGCTATACCAGCTGGATGCTTTGCCCTCTCCATACTGATTTTCTTGCTCGGCCGAAAATTGTACATTCGTGTTAAGCCCCAAGGAAGCACCTTTGGGGACATAATGAGGGTAATTGTAGCTGCTTTTCGTAAGTTTAGAACAAAGACTTCAGGGCAGTCCCTTTATGTTGCTCCTTCAATTGGACAAGAGTTGGAGCAAACACTTGCTCACACAGAGAGGTTCAAGTTTCTTGATAAGGCTGCTGTAATTGTAGATCCAAATGAATTGGATAGCAATGGCAACCCTAAAAGCGGTTGGAGACTATGCAGTGTGCAACAAGTTGAGCAACTCAAATGCGTCATAGCTTTGTTACCTGTTTGTATAAGTGGAATTGGTTGTTTCATAGGAATGCAGCAAATGCCATCATTTGGGACCTTTCAAGCTATTCAAACAAACAAAAAGATTGGAAACAATTTTGAAATCCCACCAGCCTGGATGGGTCTCACACAAATGATTGCACTTTCAATATGGATCATCATCTACGAATGCATTTACATCCCGTGGATTCAGAAAAGGAACAATGATGAATCTGGAAGATTGACAATGGAGACGAGATTCAAGGTTGGCATTGTGATGTCAATTCTGTGCATGGTGGTGGCTGGACTCACCGAAATGAAGCGCAGAAACTCTGCTTTGGAAAACAGATCTTTCGAGTCACCTATAACTGTTGCATTGCTTGTGCCACAATTTGCCTTGTCAGGTTTGATCGAGGCATTTGCAGCCATTGCCTTAATGGAGTTGCTTACAACACAGTGGCCAAAGAGCATGAGGACTTTTGCCGGGGCGGTCTTCTTTCTCAGCTTATCCATTGCAAGCTACTTCACCTCCATTCTTGTTAGCATTGTCAAGAAAGTGACTAGTTTGAATGGCAATACATCATGGCTGGGTGGTAATGACCTAAACAAGAACAGGCTTGATTACTACTATTACACCATTGCTGGTCTCGGGGTCTTGAACTTGTTGTATTTCCAATTCATTGCACGCCATTTTCTGTCCGATGCCGATGGTCACGACCGTTCTAGAAGTGATGAAGAAAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

578

Amino Acids

64.04

Weight (kDa)

8.59

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 50 - 503 3.9e-06 Major Facilitator Superfamily
PTR2 PF00854 98 - 526 5.3e-72 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 420, 1061
AccI GTMKAC 1 cut(s) 618
AciI CCGC 2 cut(s) 945, 1478
AclWI GGATC 3 cut(s) 475, 902, 1172
AcoI YGGCCR 2 cut(s) 703, 1448
AcuI CTGAAG 1 cut(s) 786
AfaI GTAC 3 cut(s) 293, 572, 716
AfiI CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
AgsI TTSAA 9 cut(s) 831, 877, 1073, 1082, 1112, 1158, 1252, 1558, 1648
AhlI ACTAGT 1 cut(s) 1550
AjnI CCWGG 1 cut(s) 1124
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 5 cut(s) 113, 946, 1139, 1235, 1640
AlwI GGATC 3 cut(s) 475, 902, 1172
Ama87I CYCGRG 1 cut(s) 1637
AoxI GGCC 4 cut(s) 339, 475, 703, 1448
ApeKI GCWGC 4 cut(s) 773, 893, 1045, 1412
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AspLEI GCGC 2 cut(s) 400, 1314
AspS9I GGNCC 1 cut(s) 1065
AsuC2I CCSGG 1 cut(s) 1474
AsuHPI GGTGA 4 cut(s) 574, 1291, 1341, 1507
AvaI CYCGRG 1 cut(s) 1637
AvaII GGWCC 1 cut(s) 1065
BalI TGGCCA 1 cut(s) 1450
BbsI GAAGAC 1 cut(s) 1474
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 4 cut(s) 760, 880, 1057, 1424
BccI CCATC 3 cut(s) 1063, 1123, 1691
BceAI ACGGC 1 cut(s) 80
BcgI CGANNNNNNTGC 2 cut(s) 1391, 1425
BciT130I CCWGG 1 cut(s) 1126
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 1474
BcoDI GTCTC 5 cut(s) 113, 946, 1139, 1235, 1640
BcuI ACTAGT 1 cut(s) 1550
BfaI CTAG 3 cut(s) 548, 1551, 1713
BglII AGATCT 1 cut(s) 1337
BisI GCNGC 4 cut(s) 774, 894, 1046, 1413
BlsI GCNGC 4 cut(s) 775, 895, 1047, 1414
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 1126, 1474
Bme18I GGWCC 1 cut(s) 1065
BmeT110I CYCGRG 1 cut(s) 1637
BmgT120I GGNCC 1 cut(s) 1065
BmiI GGNNCC 1 cut(s) 1066
BmrFI CCNGG 2 cut(s) 1126, 1474
BmsI GCATC 3 cut(s) 329, 659, 1681
BpiI GAAGAC 1 cut(s) 1474
BpmI CTGGAG 1 cut(s) 492
BpuMI CCSGG 1 cut(s) 1474
BsaBI GATNNNNATC 1 cut(s) 1169
BsaI GGTCTC 3 cut(s) 113, 1139, 1640
BsaJI CCNNGG 4 cut(s) 109, 733, 1193, 1473
BsaWI WCCGGW 1 cut(s) 608
BsaXI ACNNNNNCTCC 2 cut(s) 1502, 1532
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
Bse1I ACTGG 2 cut(s) 77, 394
Bse3DI GCAATG 9 cut(s) 247, 492, 585, 934, 1364, 1416, 1500, 1626, 1668
Bse8I GATNNNNATC 1 cut(s) 1169
BseBI CCWGG 1 cut(s) 1126
BseDI CCNNGG 4 cut(s) 109, 733, 1193, 1473
BseGI GGATG 6 cut(s) 59, 105, 320, 674, 1134, 1188
BseJI GATNNNNATC 1 cut(s) 1169
BseLI CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
BseMI GCAATG 9 cut(s) 247, 492, 585, 934, 1364, 1416, 1500, 1626, 1668
BseMII CTCAG 2 cut(s) 493, 1504
BseNI ACTGG 2 cut(s) 77, 394
BseRI GAGGAG 2 cut(s) 36, 366
BseX3I CGGCCG 1 cut(s) 703
BseXI GCAGC 4 cut(s) 760, 880, 1057, 1424
BseYI CCCAGC 2 cut(s) 639, 1576
BsgI GTGCAG 1 cut(s) 370
Bsh1285I CGRYCG 2 cut(s) 706, 1708
BshFI GGCC 4 cut(s) 341, 477, 705, 1450
BsiEI CGRYCG 2 cut(s) 706, 1708
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 1637
BsiSI CCGG 2 cut(s) 609, 1473
BslFI GGGAC 4 cut(s) 465, 764, 796, 1078
BslI CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
BsmAI GTCTC 5 cut(s) 113, 946, 1139, 1235, 1640
BsmBI CGTCTC 1 cut(s) 1235
BsmFI GGGAC 4 cut(s) 465, 764, 796, 1078
BsmI GAATGC 3 cut(s) 52, 1047, 1184
BsnI GGCC 4 cut(s) 341, 477, 705, 1450
Bso31I GGTCTC 3 cut(s) 113, 1139, 1640
BsoBI CYCGRG 1 cut(s) 1637
Bsp1286I GDGCHC 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
BspACI CCGC 2 cut(s) 945, 1478
BspANI GGCC 4 cut(s) 341, 477, 705, 1450
BspCNI CTCAG 2 cut(s) 492, 1503
BspLI GGNNCC 1 cut(s) 1066
BspPI GGATC 3 cut(s) 475, 902, 1172
BspQI GCTCTTC 1 cut(s) 532
BspTNI GGTCTC 3 cut(s) 113, 1139, 1640
BsrDI GCAATG 9 cut(s) 247, 492, 585, 934, 1364, 1416, 1500, 1626, 1668
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 77, 394
BssECI CCNNGG 4 cut(s) 109, 733, 1193, 1473
BssMI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
BssT1I CCWWGG 2 cut(s) 109, 733
Bst2UI CCWGG 1 cut(s) 1126
Bst4CI ACNGT 3 cut(s) 1360, 1446, 1709
Bst6I CTCTTC 2 cut(s) 306, 532
BstAPI GCANNNNNTGC 2 cut(s) 1051, 1418
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 4 cut(s) 42, 385, 1507, 1675
BstDEI CTNAG 2 cut(s) 479, 1490
BstDSI CCRYGG 1 cut(s) 1193
BstF5I GGATG 6 cut(s) 59, 105, 320, 674, 1134, 1188
BstHHI GCGC 2 cut(s) 400, 1314
BstKTI GATC 6 cut(s) 470, 511, 910, 1167, 1340, 1401
BstMAI GTCTC 5 cut(s) 113, 946, 1139, 1235, 1640
BstMBI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
BstMCI CGRYCG 2 cut(s) 706, 1708
BstMWI GCNNNNNNNGC 7 cut(s) 172, 269, 503, 662, 1051, 1412, 1418
BstNI CCWGG 1 cut(s) 1126
BstNSI RCATGY 1 cut(s) 44
BstSCI CCNGG 2 cut(s) 1124, 1472
BstV1I GCAGC 4 cut(s) 760, 880, 1057, 1424
BstV2I GAAGAC 1 cut(s) 1474
BstX2I RGATCY 3 cut(s) 467, 907, 1337
BstXI CCANNNNNNTGG 2 cut(s) 148, 1125
BstYI RGATCY 3 cut(s) 467, 907, 1337
BstZI CGGCCG 1 cut(s) 703
BsuRI GGCC 4 cut(s) 341, 477, 705, 1450
BtgI CCRYGG 1 cut(s) 1193
BtsCI GGATG 6 cut(s) 59, 105, 320, 674, 1134, 1188
BtsI GCAGTG 2 cut(s) 407, 967
BtsIMutI CAGTG 4 cut(s) 387, 407, 967, 1451
Cac8I GCNNGC 4 cut(s) 42, 385, 1507, 1675
CfoI GCGC 2 cut(s) 400, 1314
Cfr13I GGNCC 1 cut(s) 1065
CseI GACGC 1 cut(s) 979
Csp6I GTAC 3 cut(s) 292, 571, 715
CviAII CATG 9 cut(s) 41, 142, 237, 328, 420, 486, 1282, 1459, 1572
CviQI GTAC 3 cut(s) 292, 571, 715
DdeI CTNAG 2 cut(s) 479, 1490
DpnI GATC 6 cut(s) 469, 510, 909, 1166, 1339, 1400
DpnII GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
EaeI YGGCCR 2 cut(s) 703, 1448
EagI CGGCCG 1 cut(s) 703
Eam1104I CTCTTC 2 cut(s) 306, 532
EarI CTCTTC 2 cut(s) 306, 532
EclXI CGGCCG 1 cut(s) 703
Eco130I CCWWGG 2 cut(s) 109, 733
Eco147I AGGCCT 1 cut(s) 477
Eco31I GGTCTC 3 cut(s) 113, 1139, 1640
Eco47I GGWCC 1 cut(s) 1065
Eco52I CGGCCG 1 cut(s) 703
Eco57I CTGAAG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 1637
EcoO109I RGGNCCY 1 cut(s) 1065
EcoRII CCWGG 1 cut(s) 1124
EcoT14I CCWWGG 2 cut(s) 109, 733
EcoT22I ATGCAT 3 cut(s) 54, 421, 1184
ErhI CCWWGG 2 cut(s) 109, 733
Esp3I CGTCTC 1 cut(s) 1235
FaeI CATG 9 cut(s) 44, 145, 240, 331, 423, 489, 1285, 1462, 1575
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 4 cut(s) 465, 764, 796, 1078
FatI CATG 9 cut(s) 40, 141, 236, 327, 419, 485, 1281, 1458, 1571
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 4 cut(s) 774, 894, 1046, 1413
FokI GGATG 6 cut(s) 46, 92, 307, 681, 1141, 1175
Fsp4HI GCNGC 4 cut(s) 774, 894, 1046, 1413
FspBI CTAG 3 cut(s) 548, 1551, 1713
GlaI GCGC 2 cut(s) 399, 1313
GluI GCNGC 4 cut(s) 774, 894, 1046, 1413
GsaI CCCAGC 2 cut(s) 643, 1580
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 4 cut(s) 341, 477, 705, 1450
HapII CCGG 2 cut(s) 609, 1473
HgaI GACGC 1 cut(s) 979
HhaI GCGC 2 cut(s) 400, 1314
Hin1II CATG 9 cut(s) 44, 145, 240, 331, 423, 489, 1285, 1462, 1575
Hin6I GCGC 2 cut(s) 398, 1312
HinP1I GCGC 2 cut(s) 398, 1312
HinfI GANTC 5 cut(s) 1198, 1220, 1248, 1296, 1346
HpaII CCGG 2 cut(s) 609, 1473
HphI GGTGA 4 cut(s) 574, 1291, 1341, 1507
Hpy166II GTNNAC 3 cut(s) 362, 582, 619
Hpy188I TCNGA 6 cut(s) 83, 101, 482, 606, 1203, 1690
Hpy188III TCNNGA 7 cut(s) 471, 884, 1224, 1540, 1645, 1703, 1713
Hpy8I GTNNAC 3 cut(s) 362, 582, 619
HpyAV CCTTC 2 cut(s) 526, 837
HpyCH4III ACNGT 3 cut(s) 1360, 1446, 1709
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 269, 503, 662, 1051, 1412, 1418
HpyF3I CTNAG 2 cut(s) 479, 1490
Hsp92II CATG 9 cut(s) 44, 145, 240, 331, 423, 489, 1285, 1462, 1575
HspAI GCGC 2 cut(s) 398, 1312
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
LguI GCTCTTC 1 cut(s) 532
LmnI GCTCC 4 cut(s) 37, 503, 829, 847
Lsp1109I GCAGC 4 cut(s) 760, 880, 1057, 1424
LweI GCATC 3 cut(s) 329, 659, 1681
MaeI CTAG 3 cut(s) 548, 1551, 1713
MaeIII GTNAC 4 cut(s) 1002, 1347, 1546, 1700
MalI GATC 6 cut(s) 469, 510, 909, 1166, 1339, 1400
MboI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
MboII GAAGA 7 cut(s) 17, 48, 293, 549, 1239, 1474, 1736
MfeI CAATTG 2 cut(s) 562, 831
MflI RGATCY 3 cut(s) 467, 907, 1337
MhlI GDGCHC 1 cut(s) 544
MlsI TGGCCA 1 cut(s) 1450
MluNI TGGCCA 1 cut(s) 1450
MlyI GAGTC 2 cut(s) 1290, 1355
MmeI TCCRAC 3 cut(s) 61, 825, 929
Mox20I TGGCCA 1 cut(s) 1450
Mph1103I ATGCAT 3 cut(s) 54, 421, 1184
MscI TGGCCA 1 cut(s) 1450
MseI TTAA 4 cut(s) 254, 350, 726, 1424
MslI CAYNNNNRTG 6 cut(s) 332, 1040, 1142, 1265, 1286, 1304
Msp20I TGGCCA 1 cut(s) 1450
MspA1I CMGCKG 2 cut(s) 639, 665
MspI CCGG 2 cut(s) 609, 1473
MspR9I CCNGG 2 cut(s) 1126, 1474
MunI CAATTG 2 cut(s) 562, 831
Mva1269I GAATGC 3 cut(s) 52, 1047, 1184
MvaI CCWGG 1 cut(s) 1126
MwoI GCNNNNNNNGC 7 cut(s) 172, 269, 503, 662, 1051, 1412, 1418
NciI CCSGG 1 cut(s) 1474
NdeII GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
NlaIII CATG 9 cut(s) 44, 145, 240, 331, 423, 489, 1285, 1462, 1575
NlaIV GGNNCC 1 cut(s) 1066
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 3 cut(s) 1347, 1546, 1700
NsiI ATGCAT 3 cut(s) 54, 421, 1184
NspI RCATGY 1 cut(s) 44
PaeI GCATGC 1 cut(s) 44
PceI AGGCCT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 532
PctI GAATGC 3 cut(s) 52, 1047, 1184
PfeI GAWTC 3 cut(s) 1198, 1220, 1248
PflMI CCANNNNNTGG 2 cut(s) 420, 1061
PkrI GCNGC 4 cut(s) 775, 895, 1047, 1414
PleI GAGTC 2 cut(s) 1290, 1354
PpsI GAGTC 2 cut(s) 1290, 1354
PpuMI RGGWCCY 1 cut(s) 1065
Psp5II RGGWCCY 1 cut(s) 1065
Psp6I CCWGG 1 cut(s) 1124
PspFI CCCAGC 2 cut(s) 639, 1576
PspGI CCWGG 1 cut(s) 1124
PspN4I GGNNCC 1 cut(s) 1066
PspPI GGNCC 1 cut(s) 1065
PspPPI RGGWCCY 1 cut(s) 1065
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PsuI RGATCY 3 cut(s) 467, 907, 1337
PvuII CAGCTG 2 cut(s) 639, 665
RsaI GTAC 3 cut(s) 293, 572, 716
RsaNI GTAC 3 cut(s) 292, 571, 715
RseI CAYNNNNRTG 6 cut(s) 332, 1040, 1142, 1265, 1286, 1304
SapI GCTCTTC 1 cut(s) 532
SaqAI TTAA 4 cut(s) 254, 350, 726, 1424
SatI GCNGC 4 cut(s) 774, 894, 1046, 1413
Sau3AI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
Sau96I GGNCC 1 cut(s) 1065
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 2 cut(s) 1290, 1355
ScrFI CCNGG 2 cut(s) 1126, 1474
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 3 cut(s) 329, 659, 1681
SinI GGWCC 1 cut(s) 1065
SmiMI CAYNNNNRTG 6 cut(s) 332, 1040, 1142, 1265, 1286, 1304
SpeI ACTAGT 1 cut(s) 1550
SphI GCATGC 1 cut(s) 44
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 2 cut(s) 945, 1478
SspMI CTAG 3 cut(s) 548, 1551, 1713
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 2 cut(s) 1124, 1472
StyI CCWWGG 2 cut(s) 109, 733
TaaI ACNGT 3 cut(s) 1360, 1446, 1709
TaqI TCGA 3 cut(s) 516, 1344, 1401
TatI WGTACW 2 cut(s) 291, 714
TfiI GAWTC 3 cut(s) 1198, 1220, 1248
Tru1I TTAA 4 cut(s) 254, 350, 726, 1424
Tru9I TTAA 4 cut(s) 254, 350, 726, 1424
TscAI CASTG 4 cut(s) 394, 407, 967, 1451
TseFI GTSAC 3 cut(s) 1347, 1546, 1700
TseI GCWGC 4 cut(s) 773, 893, 1045, 1412
Tsp45I GTSAC 3 cut(s) 1347, 1546, 1700
TspDTI ATGAA 8 cut(s) 48, 225, 930, 1024, 1233, 1322, 1657, 1737
TspRI CASTG 4 cut(s) 394, 407, 967, 1451
Van91I CCANNNNNTGG 2 cut(s) 420, 1061
VpaK11BI GGWCC 1 cut(s) 1065
XbaI TCTAGA 1 cut(s) 1712
XceI RCATGY 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 4 cut(s) 84, 148, 187, 1423
XmiI GTMKAC 1 cut(s) 618
XspI CTAG 3 cut(s) 548, 1551, 1713
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 3 cut(s) 54, 421, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.